4 results about "CagA" patented technology

The present application relates to a fusion protein and its application in detection of Helicobacter pylori CagA antibody, and belongs to the technical field of biology.The present application provides a fusion protein, wherein the fusion protein comprises a polypeptide with an amino acid sequence as shown in SEQ ID NO.1, or a derivative polypeptide with more than 80% homology with the amino acid sequence as shown in SEQ ID NO.1 and capable of being specifically combined with Helicobacter pylori CagA antibody; the fusion protein is capable of being specifically combined with Helicobacter pylori CagA antibody, and has a very high application prospect in detection of Helicobacter pylori CagA antibody.

: Title: Antibacterial Application of Pyrrolidin-Piperdine Derivatives Containing Composition to Inhibit Helicobacter Pylori Replication, Preparation Method Thereof The present disclosure proposes a method for identification, optimizing, and preparing a therapeutic molecule (MA01027) for inhibiting cytotoxin-associated gene A (CagA) protein of Helicobacter pylori. The therapeutic molecule specifically targets and binds to a particular protein of interest, thereby enhancing the efficacy and specificity of the treatment. The therapeutic molecule is optimized for its binding affinity and selectivity for the target protein. The therapeutic molecule involves combining specific reactants under controlled conditions to achieve high yields and purity of the therapeutic molecule. The therapeutic molecule preparation includes purification steps to isolate the therapeutic molecule from the reaction mixture using standard techniques, thereby ensuring a high-quality final product. The therapeutic molecule binds to the CagA protein of H. pylori, inhibiting its replication by more than 90% and eliminating H. pylori-associated hemolysis.

This invention provides a multiplex nucleic acid detection primer for Helicobacter pylori, including primers targeting Helicobacter pylori 16S rRNA, shit , cow Gene-designed 16S rRNA primer pairs, shit Primer pairs, cow Primer pairs. This invention also provides detection probes, including gold nanoparticle capture probes, 16S rRNA capture probes, and... shit Capture probe, cow Capture probes and quality control capture probes. This invention also provides the above primers and probes for the preparation of Helicobacter pylori 16S rRNA, shit , cow Applications of gene detection products, as well as detection kits and methods. This invention can detect Helicobacter pylori 16S rRNA. 、cagA , cow Simultaneous detection of three genes and virulence typing, with highly specific primers and probes, stable amplification, high sensitivity, simple operation, short time consumption, and low cost, is suitable for rapid screening in clinical settings and on-site.

The application relates to the field of biotechnology, in particular to a Helicobacter pylori antibody typing reagent combination and a preparation method and application thereof, a magnetic particle chemiluminescence reagent combination for Helicobacter pylori antibody typing, which comprises an acridinium ester mouse anti-human IgG complex solution, a biotin-Ure recombinant protein complex solution, a biotin-CVfp fusion protein complex solution and a streptavidin magnetic bead mother liquor; the biotin-Ure recombinant protein complex solution is obtained by marking Ure recombinant protein with biotin, the amino acid sequence of the Ure recombinant protein is shown as SEQ ID No. 1; the biotin-CVfp fusion protein complex solution is obtained by marking CVfp fusion protein with biotin, the CVfp fusion protein is fused and expressed to antigen epitopes of CagA and VacA, the amino acid sequence of the CVfp fusion protein is shown as SEQ ID No. 2, and the problems of complex detection indexes, high reagent cost and low detection flux for current Hp virulence factor typing are solved.