Helicobacter pylori identification and virulence multiplex gene detection system, kit adopting detection system and application of detection system

A technology for Helicobacter pylori and gene detection, applied in the biological field, can solve the problems of low sensitivity, low throughput, and few detection sites, and achieve the effects of avoiding mutual interference, making up for low throughput, and reducing false positives.

Active Publication Date: 2016-04-06
HUADONG HOSPITAL +1
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that it does not reflect current infection
5) Nucleic acid analysis methods: including sequencing, PCR, oligonucleotide probe hybridization, etc., but these inspection methods have few detection sites, low specificity, low throughput, high cost, and cannot be used for quantitative analysis
However, the current conventional detection methods have shortcomings such as long time, low sensitivity, high cost, low throughput, and in particular, the inability to detect multiple related factors at the same time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Helicobacter pylori identification and virulence multiplex gene detection system, kit adopting detection system and application of detection system
  • Helicobacter pylori identification and virulence multiplex gene detection system, kit adopting detection system and application of detection system
  • Helicobacter pylori identification and virulence multiplex gene detection system, kit adopting detection system and application of detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. The composition of the kit

[0051] The Helicobacter pylori detection kit of this example includes: primer mixture, PCR buffer (10×PCRBuffer), MgCl 2 solution, dNTPs, fluorescent Universal Labeling Mix, hot-start DNA polymerase (TaqDNA Polymerase), positive and negative controls. 2mmol / L dNTPs and fluorescent universal labeling mixture are mixed together into a tube of reagents.

[0052] PCR buffer, dNTPs and hot-start DNA polymerase were all from Takara (Catalog No.: R007A).

[0053] The positive control solution is a plasmid mix that includes all gene targets of interest.

[0054] The negative control solution was nuclease-free ultrapure water.

[0055] The primer mixture includes a forward primer for the 16SrRNA gene, a reverse primer for the 16SrRNA gene, a forward primer for the cagA gene, a reverse primer for the cagA gene, a forward primer for the vacA-s1 or vacA-s2 gene, Reverse primer for vacA-s1 or vacA-s2 gene, forward primer for vacA-m1 gene, reverse ...

Embodiment 2

[0114] The Helicobacter pylori detection kit of this example is the same as the rest of Example 1, except that the primer mixture only includes the forward primer for the cagA gene, the reverse primer for the cagA gene, and the vacA-s1 or vacA -Forward primer for s2 gene, reverse primer for vacA-s1 or vacA-s2 gene, forward primer for vacA-m1 gene, reverse primer for vacA-m1 gene, forward primer for vacA-m2 gene Primers, reverse primer for vacA-m2 gene, forward primer for iceA1 gene, reverse primer for iceA1 gene, forward primer for iceA2 gene, reverse primer for iceA2 gene, forward primer for dupA gene Primers, reverse primer for dupA gene, forward primer for oipA gene, reverse primer for oipA gene, forward primer for luxS gene, reverse primer for luxS gene.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a helicobacter pylori identification and virulence multiplex gene detection system, a kit adopting the detection system and the application of the detection system. The detection system comprises a plurality of pairs of primers aiming at strain identification genes (16S rRNA) and virulence genes (cagA, vacA-s1, vacA-s2, vacA-m1, vacA-m2, iceA1, iceA2, dupA, oipA and luxS) respectively. According to the helicobacter pylori identification and virulence multiplex gene detection system and the kit adopting the detection system, routine culture and other steps are not needed, helicobacter pylori identification and multiplex virulence analysis can be directly conducted on a tissue sample in the same reaction system, the defects of conventional detection methods of low flux, high time consumption and low detection rate are overcome, comprehensive, accurate and low-cost etiology basis is provided for clinical application quickly, and important reference is provided for helicobacter pylori infection accurate diagnosis and differential diagnosis and disease prognosis.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic, campylobacter that mainly resides in the human stomach. Helicobacter pylori infection is closely related to the occurrence and development of chronic atrophic gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer, so it has attracted widespread clinical attention. In 1994, the International Agency for Research on Cancer (IARC) listed it as a human I carcinogen, and it is the only bacterial pathogenic microorganism listed as a clear carcinogen to humans so far. Many reports believe that Helicobacter pylori infection is related to coronary heart disease, rheumatoid, hepatobiliary disease, tuberculosis, pregnancy vomiting, colorectal cancer and various skin diseases, and its ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/06C12Q1/04C12R1/01
CPCC12Q1/6851C12Q1/6858C12Q1/689C12Q2600/106C12Q2600/16C12Q2537/143C12Q2545/113C12Q2565/125
Inventor 赵虎张艳梅杨长青边海鹏胡彬婕赵付菊吴勇王诗雯缪应新徐玲丽孔咪咪张景皓姜文荣陈飞
Owner HUADONG HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap