742 results about "Reverse primer" patented technology

The invention relates to an STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of the STON1 gene molecular marker primer, and belongs to the technical field of biology. The STON1 gene molecular marker is a 7867409th basic group of a chicken reference genome GRCg7b version chromosome 3, the basic group is mutated into A or G, and the genotypes are A / A, A / G and G / G. The double-yolk egg ratio of the G / G genotype chicken is higher than that of the A / G and A / A genotype individuals, and the double-yolk egg ratio of the A / G genotype chicken is higher than that of the A / A genotype individuals. A forward and reverse primer pair is used for amplifying a fragment containing the molecular marker site, Sanger sequencing is carried out on an amplification product, the genotype of an individual is rapidly and accurately identified to serve as a genetic marker for chicken breeding, and the chicken with the higher proportion of double-yolk eggs is bred; the double-yolk egg traits can be efficiently and accurately detected, the breeding accuracy and breeding efficiency of the chicken double-yolk egg proportion are improved, and the method has important value for chicken breeding and production.

The invention relates to a composition for identifying bladder cancer based on urine sample detection and application thereof, and belongs to the technical field of molecular biology. According to the composition disclosed by the invention, the bladder cancer is identified by detecting the expression of one or more of hsa-miR-423-5p, hsa-miR-155-5p and hsa-miR-590-5p in a urine sample; the composition comprises a specific reverse transcription stem-loop primer, a specific detection forward primer, a specific detection probe and a universal reverse primer. According to the composition disclosed by the invention, the expression of hsa-miR-423-5p, hsa-miR-155-5p and hsa-miR-590-5p in a urine sample is detected, and a binomial logistic regression model is combined, so that auxiliary judgment is performed on the bladder cancer, the sensitivity and the specificity are relatively high, and the accuracy of identifying the bladder cancer can be improved. The invention further provides a kit containing the composition, the detection object of the kit is a urine sample, and the kit has the advantages of being small in sample dosage, noninvasive in sampling, convenient, rapid, small in risk and the like.

The invention discloses a kit for visually detecting an H5 subtype avian influenza virus based on an RPA-CRISPR / Cas12b system and application of the kit, and belongs to the technical field of molecular detection. The kit comprises an RT-RPA (Reverse Transcription-Recombinase Polymerase Amplification) primer pair and sgRNA1 The RT-RPA primer pair comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.2; and the nucleotide sequence of the sgRNA1 is as shown in SEQ ID NO. 7. According to the kit, nucleic acid amplification and detection are synchronously completed through a tubular reaction system, the detection sensitivity reaches 1 copy / mu L, the coincidence rate with fluorescent RT-PCR is 100%, and no cross reaction exists on H7, H9, NDV and IBV. The kit does not need a complex instrument, is suitable for rapid screening on a basic level site, and provides an efficient solution for prevention and control of avian influenza.

The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses, a detection method and application, and particularly belongs to the technical field of porcine pathogen detection products. The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses. The primer probe group comprises universal forward primers PRRSV1-F and PRRSV2-F for detection, reverse primers PRRSV1-R and PRRSV2-R for detection and a universal probe PRRSV-P. A target gene detected by the primer probe group is a 3'non-coding region gene (3 'UTR) of a porcine reproductive and respiratory syndrome virus genome, and the primer probe group can realize efficient detection of American strains and European strains of the porcine reproductive and respiratory syndrome virus, and is simple and convenient in detection, high in sensitivity, good in specificity, high in speed, low in false positive and capable of realizing high-throughput detection.

The invention relates to a composition for identifying oral squamous cell carcinoma based on saliva sample detection and application, and belongs to the technical field of molecular biology. The composition provided by the invention is used for identifying the oral squamous cell carcinoma by detecting the expression of SNORD52 and / or SNORD65 in a saliva sample; the composition comprises a specific reverse transcription stem-loop primer, a specific detection forward primer, a specific detection probe and a universal reverse primer. According to the composition disclosed by the invention, the expression of SNORD52 and / or SNORD65 in a saliva sample is detected, and a binomial logistic regression model is combined to perform auxiliary judgment on the oral squamous cell carcinoma, so that the composition has relatively high sensitivity and specificity, and the identification accuracy of the oral squamous cell carcinoma can be improved. The invention further provides a kit containing the composition, a detection object of the kit is a saliva sample, and the kit has the advantages of being small in sample dosage, noninvasive in sampling, convenient, rapid, small in risk and the like.

The invention relates to a primer group for detecting pangolin plague virus, a kit and application, and relates to the technical field of gene engineering. The primer group comprises a forward primer ORF1-F and a reverse primer ORF1-R. The forward primer ORF1-F and the reverse primer ORF1-R. On the basis of the primer group, the E gene in the conserved region of the pangolin plague virus can be specifically recognized, so that the primer group is combined with an RAA amplification system and a detection system, and detection is rapidly completed within a short time under the constant-temperature condition.

The invention relates to a CCDC171 gene molecular marker primer related to egg weight character and application of the CCDC171 gene molecular marker primer, and belongs to the technical field of biology. The CCDC171 gene molecular marker is located at the 32551904 base of a Z chromosome of a chicken reference genome GRCg7b version, the base is mutated into C or T, and the genotypes are C / C and T / T. And the egg weight of the C / C genotype chicken is higher than that of the T / T genotype individual. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper egg weight; the egg weight character can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for chicken breeding and production.

The invention discloses a primer probe group and a kit for detecting southern rust pathogenic bacteria, namely puccinia polypoda, based on MIRA-LFIA and application of the primer probe group and the kit. The primer probe group comprises a forward primer, a reverse primer and a probe combination, and the nucleotide sequence of the forward primer is as shown in SEQ ID NO.1; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 2; the nucleic acid sequence of the probe primer is as shown in SEQ ID NO. 3. The MIRA-LFIA detection method of the pathogenic bacteria is established on the basis of the primers, and the detection method has good specificity and sensitivity, short detection time and low cost, realizes visualization of interpretation results by combining lateral flow immunochromatography color development, and is especially suitable for field real-time monitoring. The detection method provided by the invention lays a foundation for early diagnosis of puccinia multiflora and development of a monitoring technology of pathogenic bacteria, and provides guarantee for high yield of corn.

The invention relates to a PPP3R1 gene molecular marker primer related to the size character of a cockscomb and application of the PPP3R1 gene molecular marker primer, and belongs to the technical field of biology. The molecular marker of the PPP3R1 gene is located at the 11347456 base of chromosome 3 of a chicken reference genome GRCg7b version, the base is mutated into T or G, and the genotypes are G / G, T / G and T / T. The cockscombs of the G / G genotype chickens are larger than those of the T / G and T / T genotype individuals, and the cockscombs of the T / G genotype chickens are larger than those of the T / T genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper cockscomb size; the method can efficiently and accurately detect the size of the cockscomb of the chicken, improves the uniformity of the cockscomb of a group, and has important value for breeding and production of the chicken.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention discloses an RT-RPA (reverse transcription recombinase polymerase amplification) and CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a (CRISPR / Cas12a) based rapid detection system and a kit and a use method of the kit. The virulent virus is a Congo fever virus, a Rift Valley fever virus, a Nipah virus or a Hendea virus; the detection system comprises an RT-RPA isothermal amplification system and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a trans-cleavage detection system. The RT-RPA isothermal amplification system comprises an RT-RPA forward primer and an RT-RPA reverse primer which are designed on the basis of viral nucleic acid; the CRISPR / Cas12a trans-cleavage detection system comprises a crRNA sequence which is designed on the basis of viral nucleic acid. The kit comprises the RT-RPA isothermal amplification system and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a (CRISPR / Cas12a) trans-cleavage detection system. The four virulent virus nucleic acids are detected on the basis of RT-RPA and CRISPR / Cas12a systems for the first time, and the kit has the advantages of being high in specificity, high in sensitivity, simple, rapid, independent of complex large instruments and the like, and can be widely applied to clinical sample detection.

The invention discloses a specific detection target PsRrp8 of phytophthora sojae, a DNA (deoxyribonucleic acid) sequence of the specific detection target PsRrp8 is shown as SEQ ID NO.1. The invention further provides a primer combination for detecting the phytophthora sojae, the primer combination comprises a forward primer and a reverse primer, the sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO.2, and the sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO.3. The invention further provides a kit for detecting the phytophthora sojae. The lowest detectable DNA concentration of the detection primer combination provided by the invention on the phytophthora sojae (P.sojae) is 0.332 pg.L <-1 >, which indicates that the quantitative detection technology has high sensitivity. The method can be successfully applied to phytophthora detection of phytophthora root rot disease field plants, phytophthora sojae can be rapidly detected, and a new technical means is provided for early warning of diseases.

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention relates to the technical field of crop molecular breeding and detection, in particular to a sweet waxy corn molecular detection system based on an sh1 gene InDel marker and application of the sweet waxy corn molecular detection system, the sweet waxy corn molecular detection system is composed of a forward primer, a reverse primer and a transposon specific primer, and the sequences are shown as SEQ ID NO: 1-3. The sh1 gene InDel marker-based sweet waxy corn molecular detection system provided by the invention can be used for corn sh1 gene Mu insertion rapid detection and / or corn molecular marker-assisted sweet waxy corn breeding. By adopting a sweet waxy corn molecular detection system based on the sh1 gene InDel marker, the accuracy rate is 98.7%. And the detection cost of each sample is 0.15 dollars, and compared with the traditional SSR marking technology, the cost is reduced to 3% of that of the traditional SSR marking technology. From DNA extraction to final result interpretation, the whole detection process takes 3 hours.

The invention provides a primer group and a kit for detecting bovine mycobacterium paratuberculosis based on a recombinase isothermal amplification technology and application, and relates to the technical field of biological detection.The primer group comprises a forward primer MAP-F3, a reverse primer MAP-R3 and a specific fluorescent probe MAP-P3 and has good specificity; the sensitivity reaches 10fg / mu L, which is more sensitive than that of a colloidal gold test strip, the dependence on equipment is broken through, and the detection result is more accurate. The technical problem that in the prior art, it is difficult to distinguish bovine mycobacterium paratuberculosis from other pathogens through a detection method, or the detection process is tedious is solved. According to the prepared reagent or kit, the liquid preparation process is simple, the whole amplification process can be completed only in 30 min, the amplification yield can reach 109-1010 copies, the method is rapid and efficient, the amplification result can be directly judged according to fluorescence data, other auxiliary means are not needed, and the method is suitable for field detection.

The invention provides a foot and mouth disease virus detection primer probe and a microdroplet digital PCR absolute quantitative detection kit thereof, and belongs to the technical field of biological detection. The foot and mouth disease virus detection primer probe comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a probe with a nucleotide sequence as shown in SEQ ID NO: 3. The invention also provides a foot-and-mouth disease virus microdroplet digital PCR absolute quantitative detection kit. The foot-and-mouth disease virus microdroplet digital PCR absolute quantitative detection kit comprises the foot-and-mouth disease virus detection primer probe, a 5 * one-step probe RT-qPCR main mixed solution, RT-Polymerase Mix, a reference dye, sealing oil and a digital PCR chip. The kit provided by the invention has the characteristics of high sensitivity, strong specificity, high accuracy and high precision, and can realize accurate and quantitative chip digital PCR detection.

The invention provides a primer group and a kit for identifying corn varieties and application of the primer group and the kit. The primer group comprises a first primer pair to a 200th primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair to the forward primer of the 200th primer pair and the reverse primer of the 200th primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 400 in a sequence table. DNA fingerprint data of to-be-detected samples can be obtained through multiple PCR amplification and high-throughput sequencing by utilizing the primer group, then a variety identification conclusion is obtained by comparing the DNA fingerprint data of the to-be-detected samples, the accuracy and the digitization degree are high, hundreds of to-be-detected samples can be compared at a time through sequence analysis software, the variety identification conclusion is rapidly obtained, and the method is suitable for large-scale popularization and application. The accuracy and efficiency of corn variety identification can be obviously improved.

The invention discloses a kit for screening and diagnosing colorectal cancer, and belongs to the technical field of medical detection. The kit for screening and diagnosing the colorectal cancer comprises a forward primer and a reverse primer, wherein the forward primer and the reverse primer are used for detecting tsRNA-Gly-5-0007; the sequence of the forward primer is as shown in SEQ ID NO.2, and the sequence of the reverse primer is as shown in SEQ ID NO.3. Compared with healthy volunteers, the tsRNA-Gly-5-0007 expression level in serum exosomes of colorectal cancer patients is remarkably reduced for the first time, ROC curve analysis shows that AUC is 0.795, sensitivity is 72.7%, and specificity is 75%; compared with healthy volunteers, the expression level of tsRNA-Gly-5-0007 in serum exosomes of patients with early colorectal cancer is remarkably reduced; rOC curve analysis shows that AUC is 0.788, the sensitivity is 70%, and the specificity is 78.7%.

The invention discloses a mycoplasma hyorhinis detection primer and kit based on CRISPR-Cas13a and application of the mycoplasma hyorhinis detection primer and kit, and belongs to the technical field of mycoplasma hyorhinis detection. The invention discloses an RAA primer pair for detecting mycoplasma hyorhinis, the RAA primer pair comprises a forward primer and a reverse primer, the nucleotide sequence of the forward primer is as shown in SEQ ID NO.15, and the nucleotide sequence of the reverse primer is as shown in SEQ ID NO.16. The invention further discloses a kit for detecting mycoplasma hyorhinis. And an efficient, convenient and high-specificity in-situ rapid detection method is developed to meet the actual requirements of mycoplasma hyorhinis diagnosis.

The invention relates to a primer of a GAREM1 gene molecular marker related to the average daily gain character of pigs and application of the primer, and belongs to the technical field of biology. The GAREM1 gene molecular marker is located at the 116131455th basic group of chromosome 6 of a pig reference genome Sscrofa11.1 version, the basic group is mutated into A, and the genotypes are A / A and A / T. The average daily gain of A / T genotype pigs is higher than that of A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for pig breeding to breed pigs with proper weight; the method can be used for efficiently and accurately detecting the average daily gain character of the pigs, and has an important value for breeding and production of the pigs.

The invention relates to the technical field of sweet potato virus detection, and provides a sweet potato virus G detection method. The invention relates to a sweet potato virus G specific primer pair. The nucleotide sequence of a forward primer SPVG-F of the specific primer pair is as shown in SEQ ID NO: 1; and the nucleotide sequence of the reverse primer SPVG-R is as shown in SEQ ID NO: 2. According to the technical scheme, the problems of low detection sensitivity and low detection efficiency when a sweet potato G virus amplification primer and a sweet potato G virus same family virus compositely infect a sample in the prior art are solved.

The invention discloses a primer for rapidly detecting fish rhabdovirus CAPRV2023 on site based on a dual signal amplification technology and application of the primer, the primer comprises a DNA forward primer F2, a DNA reverse primer R2 and an RNA primer RNA4, the sequences of the DNA forward primer F2, the DNA reverse primer R2 and the RNA primer RNA4 are respectively as shown in SEQ ID NO: 2, SEQ ID NO: 14 and SEQ ID NO: 10, the 5'end of the RNA primer RNA4 is an FAM labeled reporter group, and the 3 'end of the RNA primer RNA4 is a BHQ1 labeled quenching group. The invention also discloses an application of the primer or the kit in on-site rapid detection of the fish rhabdovirus CAPRV2023 with a non-disease diagnosis purpose. The kit disclosed by the invention can be used for efficiently and quickly detecting a sample, is high in specificity and can be used for discovering the sample infected with CAPRV2023 as soon as possible.

The invention provides an SV molecular marker related to cassava branched chain and amylose content and application, and belongs to the technical field of molecular biology, the SV molecular marker is Chr142634494926345058 and is used for predicting the cassava branched chain and / or amylose content; a primer group for amplifying the SV molecular marker comprises a forward primer, a reverse primer, a forward primer, a reverse primer and a reverse primer, wherein the sequence of the forward primer is 5 '-AGATCATACAGCCCATTTT-3'; the sequence of the reverse primer is 5 '-ATCCACCAAAGTTCGTAA-3', and the sequence of the reverse primer is 5 '- The primer group is used for preparing the kit; the primer group or the kit is used for carrying out PCR amplification on the DNA of the cassava to be detected so as to predict the branched chain and / or amylose content of the cassava. The SV molecular marker provided by the invention can accurately and efficiently predict the high or low content of branched chain and amylose of cassava without planting and waiting for cassava adult plants, and greatly improves the breeding selection efficiency.

The invention relates to the technical field of pathogenic microorganism detection, in particular to a method for quantitatively detecting chlamydia psittaci based on microdroplet type digital PCR (Polymerase Chain Reaction). The invention discloses a primer probe composition for detecting chlamydia psittaci. The primer probe composition consists of a forward primer, a reverse primer and a probe, the nucleotide sequence of the forward primer is as shown in SEQ ID NO: 1; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO: 2; the nucleotide sequence of the probe is as shown in SEQ ID NO: 3. The detection method provided by the invention is high in sensitivity, strong in specificity, rapid in detection and simple and convenient to operate, the used primer pair and probe are optimally designed based on sequence site difference, the detection background value is low, and the result is easier to judge.

The invention is suitable for the technical field of soybean breeding, and provides a KASP molecular marker for soybean plant height identification and application of the KASP molecular marker. The KASP molecular marker is based on an SNP variation site of a Glyma. 19G206100 gene between two parents of Chinese flat stem soybean and F02, the mutation position of the Glyma. 19G206100 gene is the 312th basic group on a CDS sequence, C of a reference genome is mutated into G, the 104th amino acid on a protein sequence is mutated into lysine from asparagine, and the 104th amino acid on the protein sequence is mutated into lysine from asparagine. The primer of the KASP molecular marker comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1-2 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.3. The invention further discloses a kit for detecting the KASP molecular marker. The KASP molecular marker is developed on the basis of the polymorphic site of Gm19G206100 in germplasm resources, the marker has the advantages of being high in resolution ratio and easy to detect, can be applied to plant height selection of early generations of soybean cross breeding, has the effects of reducing the breeding scale, reducing the workload and improving the breeding efficiency, and has important application value in production.

The invention provides a primer / probe composition for capillary electrophoresis analysis, a detection method and application. The primer / probe composition comprises a forward primer, a reverse primer and a nucleic acid probe, the 5'end of the nucleic acid probe carries a fluorescent label, and both the forward primer and the reverse primer do not carry the fluorescent label. Different targets are identified by detecting the nucleic acid probe, so that the length of an amplification product is not limited, only the nucleic acid probe carries a fluorescence signal without generating a non-specific fluorescence signal, the generation of a supersaturated fluorescence signal can be effectively prevented by using the nucleic acid probe with a specific concentration, the fluorescence signals among different targets do not interfere with each other, and the detection sensitivity is high. And multicolor fluorescence can be used at the same time.

The invention belongs to the technical field of gene engineering, and discloses a preparation method and application of small-fragment DNA and the small-fragment DNA. According to the preparation method of the small-fragment DNA, provided by the invention, a forward fragment group and a reverse primer group which are obtained by carrying out segmentation design on a positive-sense strand and an antisense strand of the small-fragment DNA are used for replacing primers and template DNA which are conventionally used in existing PCR amplification, and the capability of forming a dimer between a forward fragment fragment and a reverse fragment fragment is ingeniously utilized; a PCR amplification reaction system contains a complete nucleotide sequence required by amplification to obtain small-fragment DNA, and a dimer formed by a forward fragment fragment and a reverse fragment fragment has a free fragment and can extend in a PCR amplification cycle so as to gradually form the complete small-fragment DNA, so that the small-fragment DNA can be obtained. The method can effectively overcome the limitation on the design of the primer caused by the short DNA length of the small fragment in the existing PCR amplification method, can quickly, efficiently and accurately amplify the DNA of the small fragment, and has a good application prospect in the genetic engineering technology.

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker related to main inflorescence pod density (SDMI) and application of the molecular marker, the sequence of a forward primer for amplifying the molecular marker is 5 '-CCTAACCACCTACGCTGAG TTATATATAGATT-3', and the sequence of a reverse primer for amplifying the molecular marker is 5 '-TAG TCTGTAAATTTATTATGAGCTTCCTTGTCTT-3'. The total DNA (deoxyribonucleic acid) of a single rape plant is used as a template, and PCR (polymerase chain reaction) amplification is carried out by using the primer sequence, so that two bands of 279-bp and 254-bp can be obtained; the rape single plant only having a 279-bp single band is a sparse fruit individual, and the rape single plant only having a 254-bp specific band is a dense fruit individual. The molecular marker developed by the invention can be used for identifying and predicting the pod density, the operation is simple and convenient, the method is easy to implement, the offspring selection is more targeted, and the selection efficiency is improved.

The invention relates to a primer group, a kit and a method for detecting an ATXN3 gene (CAG) n trinucleotide repetition number, and belongs to the technical field of gene detection. The technical problem to be solved by the invention is to provide the primer group for detecting the n trinucleotide repetition number of the ATXN3 gene (CAG). The primer group comprises a first primer pair and a second primer pair, a forward primer sequence of the first primer pair is as shown in SEQ ID NO. 1, and a reverse primer sequence of the first primer pair is as shown in SEQ ID NO. 2; a forward primer sequence of the second primer pair is as shown in SEQ ID NO. 3, and a reverse primer sequence of the second primer pair is as shown in SEQ ID NO. 4. Through the design of the two pairs of primers, complementary verification is achieved, the detection result is comprehensive and reliable, the technical defects of first-generation sequencing and second-generation sequencing on long-fragment and high-GC-content repetitive sequence detection are overcome, the detection cost is low, the detection period is short, and the primer pair is suitable for clinical detection service.

The invention relates to the field of molecular biology, and discloses an SNP molecular marker set for identifying the variety of Chinese pears, the SNP molecular marker set is a specific SNP molecular marker set, the specific molecular marker set comprises 30 SNP markers, the invention also provides a KASP primer combination for amplifying the SNP molecular marker set, the KASP primer combination is composed of detection primers of the 30 SNP sites, and the KASP primer combination is used for amplifying the variety of Chinese pears. The specific primer for detecting each SNP site is composed of two forward primers and one reverse primer. The method has the following advantages and effects that compared with a variety identification technology based on field trials, the latest SNP marking technology and KASP genetic typing technology for international variety identification are not influenced by human subjectivity, climate environment and seasons; compared with an SSR molecular marker, the result is more accurate, the detection process is more convenient, and toxic chemical reagents such as acrylamide are not needed. The method makes up the blank of identifying the Chinese pear variety based on the SNP marker technology, and can be effectively used for Chinese pear variety DNA fingerprint database construction and rapid variety identification.