52 results about "Vibrio alginolyticus" patented technology

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention discloses a lytic bacteriophage vBVpaSR40Z and application of the lytic bacteriophage vBVpaSR40Z to prevention and control of vibrio parahaemolyticus in an aquaculture environment. The preservation number of the vibrio parahaemolyticus phage vBVpaSR40Z is as follows: GDMCC No: 67440-B1, and the vibrio parahaemolyticus phage vBVpaSR40Z is The R40Z can be used for cracking multiple strains of vibrio parahaemolyticus and vibrio alginolyticus, has the capability of adapting to an aquaculture environment, and keeps relatively high activity within the range of 4-25 DEG C and pH of 4-10; no virulence gene or drug-resistant gene is detected through genome analysis, and phylogenetic and whole genome comparison shows that the similarity between R40Z and a related phage is lower than a species level threshold value, so that R40Z is a new phage species; the R40Z can obviously inhibit the growth of a host under various infection complex numbers. The R40Z has the advantages of strong splitting capacity, good environmental stability, high genome safety, unique evolutionary characteristics and the like, and is an aquatic vibriosis biological prevention and control bacteriophage with application potential.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The invention provides a bacteriostatic agent containing an agilawood leaf extracting solution for inhibiting vibrio alginolyticus and application of the bacteriostatic agent, and relates to the field of plant extracts. The bacteriostatic agent disclosed by the invention is an agilawood leaf extracting solution; the agilawood leaf extracting solution is prepared by adding water into agilawood leaves, crushing and extracting, and the material-liquid ratio of the agilawood leaves to the water is 1g: (2-4mL); the agilawood leaf extracting solution is used for inhibiting vibrio alginolyticus. The preparation method of the agilawood leaf extracting solution comprises the following steps: taking agilawood leaves, adding water, crushing into homogenate, filtering to remove impurities, centrifugally separating, collecting supernate, and sterilizing to obtain the agilawood leaf extracting solution. The minimum inhibitory concentration of the agilawood leaf extracting solution prepared by the invention on vibrio alginolyticus is 15.63 mg / ml. The agilawood leaf extracting solution prepared by the invention can well inhibit vibrio alginolyticus, is used for preparing a bacteriostatic agent for inhibiting vibrio alginolyticus, avoids the risk of drug-resistant genes, has no risk of organic solvent residue, and has the advantages of high efficiency, risk reduction, cost reduction and the like.

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses a lytic bacteriophage vB_VpaS_R40Z and application thereof in preventing and controlling Vibrio parahaemolyticus in aquaculture environment. The Vibrio parahaemolyticus bacteriophage vB_VpaS_R40Z has a preservation number of GDMCC No: 67440-B1. The R40Z can lyse multiple strains of Vibrio parahaemolyticus and Vibrio alginolyticus, and has the ability to adapt to the aquaculture environment, and remains high activity within the range of 4-25 DEG C and pH 4-10; genome analysis does not detect virulence genes or drug resistance genes; phylogenetic comparison with the whole genome shows that the similarity of R40Z with the related bacteriophage is lower than the species level threshold, and R40Z is a new bacteriophage species; R40Z can significantly inhibit the growth of the host under multiple infection multiplicities. R40Z has the advantages of strong lytic ability, good environmental stability, high genome safety and unique evolutionary characteristics, and is a water product vibrio disease biological prevention and control bacteriophage with application potential.

The application discloses a broad-spectrum short tail phage PhLNJZ2 targeting multi-drug resistant Vibrio and an application thereof, and belongs to the technical field of microorganisms. The phage PhLNJZ2 is preserved in the China Center for Type Culture Collection on October 20, 2025, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 20252251, and the classification and naming is Vibrio phage PhLNJZ2. The phage is screened from a multi-drug resistant Vibrio alginolyticus clinical isolate which is resistant to more than 7 kinds of antibiotics, exhibits high efficient and broad-spectrum lytic activity on three main waterborne pathogenic Vibrio, i.e., Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi, and the total lytic rate reaches 81.25%. The application further provides a composition containing the phage, a feed additive and an application of the phage in preparation of a medicine for preventing and controlling waterborne multi-drug resistant and mixed Vibrio disease or an environmental disinfectant.

The application discloses specific molecular targets of Vibrio alginolyticus and Vibrio fluvialis and a detection method thereof, wherein the nucleotide sequence of the specific molecular target of the Vibrio alginolyticus is as shown in SEQ ID NO. 1, and the nucleotide sequence of the specific molecular target of the Vibrio fluvialis is as shown in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The application not only discloses one specific molecular target for identifying the Vibrio alginolyticus and four specific molecular targets for identifying the Vibrio fluvialis, but also provides related specific primer sequences and corresponding PCR detection methods. The application has the advantages of short detection time, low cost, strong specificity and the like without physiological and biochemical identification.

The application provides a bacteriophage for controlling Vibrio infection, and belongs to the field of veterinary technology. The application designs a recombinant tail chain protein rTC and synthesizes an expression gene of the recombinant tail chain protein rTC through amino acid sequence alignment and structure analysis of tail chain proteins of Vibrio phages. The rTC gene is electroporated into Vibrio parahaemolyticus isolated from diseased prawns, and the host bacteria are infected with PHxp1 bacteriophage isolated from seawater; after the rTC gene is recombined with the PHxp1 bacteriophage, the collected bacteriophage is screened with Vibrio alginolyticus to obtain two bacteriophages PHxp1-1 and PHxp1-2 capable of lysing Vibrio alginolyticus; the bacteriophage is screened with Vibrio harveyi to obtain one bacteriophage PHxp1-3 capable of lysing Vibrio harveyi. The host spectrum of the three recombinant bacteriophages is widened. The recombinant tail chain protein bacteriophage greatly reduces the death and growth stagnation of prawns caused by Vibrio infection in prawn farms, and improves the breeding benefit.

This invention discloses the application of L-lysine in improving the sensitivity of Vibrio alginolyticus to clindamycin. This invention discovers that the combined use of L-lysine and clindamycin can significantly increase reactive oxygen species within Vibrio alginolyticus, thereby increasing the amount of clindamycin entering the bacteria and ultimately leading to bacterial death. Therefore, L-lysine can improve bacterial sensitivity to clindamycin, thus overcoming the problem of bacterial resistance. The small molecule metabolite provided by this invention, when used in combination with clindamycin, can significantly enhance the bactericidal effect of clindamycin, exhibiting better efficacy, higher safety, and greater operability compared to existing methods that use clindamycin alone as an antibacterial drug.

The invention discloses a TaqMan probe qPCR (quantitative polymerase chain reaction) detection primer group for detecting pathogenic vibrio alginolyticus, a kit and application, and belongs to the technical field of molecular biology and pathogenic microorganism detection. The method comprises the following steps: designing a pair of specific primers and probes by taking the vops gene of the vibrio alginolyticus as a new target, connecting the vops gene with plasmids by amplifying the vops gene, constructing a recombinant plasmid standard substance, carrying out qPCR detection on the copy number of the vops gene of the vibrio alginolyticus by utilizing the designed specific primers and probes, and establishing a corresponding relationship between the vibrio alginolyticus and the vops gene. And the vibrio alginolyticus can be quantified through the copy number of the vops gene. On the basis of novel molecular target detection, no amplification signal exists for a detection sample which does not contain vibrio alginolyticus, and the specificity is good; the method is rapid and accurate in detection and high in sensitivity, the lower detection limit is as low as 100-level copies, non-specific amplification can be avoided, and operation is simple and rapid.

A method of topically applying to skin a composition comprising a water soluble Phoenix dactylifera seed extract, an essential oil from the leaves of tea tree, an aqueous Myrothamnus flabellifolia leaf and stem extract, and a saccharide isomerate comprising an exopolysaccharide of Vibrio alginolyticus is disclosed.

The invention provides a feed additive capable of improving the antibacterial ability of haliotis diversicolor and a preparation method and application thereof.The preparation method of the feed additive comprises the steps that an ulva lactuca raw material is properly treated, polysaccharide components in the ulva lactuca raw material are extracted, and the obtained ulva lactuca polysaccharide is scientifically proportioned and mixed with a proper feed carrier and other nutritional ingredients; after the feed additive is fed, the ulva polysaccharide can play a role in the abalone body, enhance the non-specific and specific immune response of the abalone and improve the removal capacity of vibrio alginolyticus, so that diseases caused by pathogenic bacteria are effectively prevented and controlled, and the morbidity and mortality in the abalone culture process are reduced. By using the feed additive disclosed by the invention, the health condition and the growth performance of abalones can be improved, the use amount of antibiotics is reduced, the culture risk and the environmental pollution are reduced, the quality and the safety of abalone products can also be improved, and the feed additive has important practical significance and popularization value for promoting the sustainable development of the abalone culture industry.

The invention relates to the technical field of microorganisms, and provides a plant lactobacillus strain which is separated from an intestinal tract sample of wild fish in a Danjiangkou reservoir. Experiments prove that the strain has relatively high acid resistance and bile salt tolerance, and has certain bacteriostatic ability to vibrio parahaemolyticus, vibrio alginolyticus, aeromonas veronii, aeromonas cainea and aeromonas hydrophila. The invention also provides an aquatic feed composition comprising such a strain. The aquatic feed composition containing the DY-JY3 strain can promote growth of largemouth black bass, improve immunity and digestive enzyme activity and adjust intestinal flora, so that the aquatic feed composition has remarkable potential in the aspect of being a probiotic candidate strain in aquaculture application, and a new microbial intervention strategy is provided for anti-infection treatment of fishes. The strain and the composition provided by the invention have wide application prospects in the field of aquatic products.

The invention discloses a bacteriophage vBValPR44J with the potential of preventing and controlling vibrio diseases. The preservation number of the vibrio alginolyticus bacteriophage vBValPR44J is GDMCC No: 67435-B1, and the preservation number of the vibrio alginolyticus bacteriophage The vBValPR44J belongs to schizoviridae, but has extremely low similarity with a genome of a known related strain, and represents a new candidate virus genus. A one-step growth curve reveals that the R44J has an extremely short incubation period and efficient host lysis activity. In addition, genomic safety assessment proves that R44J does not carry any lyogen-related genes, virulence factors or antibiotic resistance-related genes. In conclusion, due to the unique taxonomic status, efficient splitting capacity and excellent genetic safety of the R44J, the R44J has a wide application prospect as a biological agent for preventing and controlling the aquaculture vibriosis.

The invention discloses a metabonomics-based method for distinguishing a vibrio alginolyticus wild type virulent strain and a crp gene deletion type weak strain. According to the method, a vibrio alginolyticus virulent strain and a crp gene deletion type attenuated strain are distinguished according to the ratio of the content of arabinose to the content of myristic acid in vibrio alginolyticus measured by metabonomics, and the vibrio alginolyticus crp gene deletion type attenuated strain with the ratio of arabinose to myristic acid in a sample to be detected being 5.9 or above is the vibrio alginolyticus crp gene deletion type attenuated strain. The vibrio alginolyticus strain with the ratio of arabinose to myristic acid being less than 1.5 is a vibrio alginolyticus wild type The method is simple to operate and reliable in detection result, and can provide reference for vibrio alginolyticus crp metabolic characteristic analysis and related vibrio alginolyticus crp metabonomics research.

The invention relates to the technical field of microorganisms, and particularly discloses a streptomyces rust red strain JQ021 and application thereof. The strain is preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number of the strain is CCTCC NO: M 20252186. The strain JQ021, a culture of the strain JQ021 and a metabolic extract produced by the strain JQ021 show efficient and broad-spectrum inhibitory activity on various aquatic pathogenic vibrios, especially vibrio parahaemolyticus, vibrio alginolyticus and vibrio Ewana. The invention also relates to a microbial preparation and an antibacterial composition containing the strain or the metabolic extract, and an application of the microbial preparation and the antibacterial composition in preparation of a preparation for preventing and treating aquaculture vibriosis. The strain provides valuable microbial resources for developing safe and efficient antibiotic substitutes.

The invention discloses a specific bacteriophage PV2-35-1 of vibrio alginolyticus and an application of the specific bacteriophage PV2-35- The Vibrio alginolyticus phage PV2-35-1 is preserved in Guangdong Province Microbial Culture Collection Center (GDMCC) on December 1, 2025, the address is the fifth floor of the building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, the postal code is 510070, and the preservation number is GDMCC No: 67384-B1. The Vibrio alginolyticus phage PV2-35-1 is named as Vibrio alginolyticus phage PV2-35-1. The head of the bacteriophage is of an icosahedron structure, the diameter is 57 + / -2 nm, the tail of the bacteriophage is a non-contractile long tail, the tail length is 154 + / -3 nm, and the bacteriophage belongs to the tailed bacteriophage and the long-tail bacteriophage family. The optimal infection complex number of the bacteriophage is 0.1, the incubation period is 40 min, the lysis period is 100 min, and the lysis amount is 248 PFU / mL.

The invention discloses a strong vibrio alginolyticus bacteriophage vBValSR43D and an application thereof. The invention relates to Vibrio alginolyticus phage vBValS R43D, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is No.100 courtyard of Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong province, the postcode is 510070, and the preservation number is GDMCC NO.67433-B1. The invention further discloses a preparation method of the Vibrio alginolyticus phage vBValS R43D. The bacteriophage R43D shows definite application potential in prevention and control of vibrio alginolyticus infection in aquaculture, and provides important strain resources and theoretical basis for subsequent development of bacteriophage preparations or cocktail therapy.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.

The invention provides an antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of the antibacterial peptide ATMP23 mutant in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M4, A23-M5 and A23-M6 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 mutant provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, and has a good bactericidal effect on clostridium welchii, staphylococcus aureus, streptococcus, vibrio parahaemolyticus, vibrio alginolyticus, vibrio splendidus, salmonella, escherichia coli and the like; when being applied to livestock and aquatic feed, the feed conversion ratio of livestock and aquatic products can be remarkably reduced, the growth performance and immune function indexes of bred animals can be improved, and the feed additive can be widely applied to the industries of feed, food, medicine and the like.

The present application relates to the field of microbiological technology, and specifically discloses a strain of Streptomyces rutilocolore JQ021 and its application. The strain has been preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number is CCTCC NO: M 20252186. The strain JQ021 and its culture, metabolic extract produced therefrom all exhibit high and broad-spectrum inhibitory activity against various aquatic pathogenic Vibrio, especially Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio owensii. The present application also relates to a microbial preparation and an antibacterial composition comprising the strain or the metabolic extract, and the application thereof in preparing a preparation for preventing and treating aquatic vibrio disease. The strain provides a valuable microbial resource for developing safe and efficient antibiotic substitutes.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

The application discloses a kind of pharmaceutical compositions. The pharmaceutical composition includes A agent and B agent;The A agent includes Vibrio parahaemolyticus bacteriophage, Vibrio harveyi bacteriophage, Vibrio alginolyticus bacteriophage and Bacteriophage of Achromobacter nematophilus;The B agent includes bacitracin, short bacitracin, polymyxin and nisin. The present application can effectively inhibit and solve the "red bottom" phenomenon that appears in the bottom of pool in the process of breeding of snail, improve the success rate of breeding of snail.

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

The application discloses a Clostridium butyricum granular preparation for pond bottom improvement, which comprises the following components: 15-20 parts of Clostridium butyricum (CCTCC M 2018426) fermentation liquor, 5-17 parts of gallnut extract liquor, 35-50 parts of montmorillonite and 5-15 parts of corn starch, wherein the viable bacterial count of Clostridium butyricum in the fermentation liquor is above 10 8 CFU / ml, and the spore rate reaches above 90%. In the scheme, Clostridium butyricum is matched with traditional Chinese medicine gallnut, and the granular preparation is formed by taking montmorillonite and corn starch as carriers. In the application of pond bottom improvement, it is found that the granular preparation can greatly inhibit the reproduction of Vibrio alginolyticus and Vibrio parahaemolyticus in a short period, and is helpful to improve the pond bottom environment.