7 results about "Vibrio alginolyticus" patented technology

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

This invention discloses the application of L-lysine in improving the sensitivity of Vibrio alginolyticus to clindamycin. This invention discovers that the combined use of L-lysine and clindamycin can significantly increase reactive oxygen species within Vibrio alginolyticus, thereby increasing the amount of clindamycin entering the bacteria and ultimately leading to bacterial death. Therefore, L-lysine can improve bacterial sensitivity to clindamycin, thus overcoming the problem of bacterial resistance. The small molecule metabolite provided by this invention, when used in combination with clindamycin, can significantly enhance the bactericidal effect of clindamycin, exhibiting better efficacy, higher safety, and greater operability compared to existing methods that use clindamycin alone as an antibacterial drug.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.

This invention relates to the field of microbial technology, specifically to a highly efficient lysing Vibrio alginolyticus phage derived from abalone, its composition, and its applications. The phage, named RKP-VA24471, has been deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 46426. The phage has a titer of not less than 1 × 10⁻⁶. ^10 With a PFU / mL concentration and an optimal MOI of 0.01, this phage exhibits advantages such as a short latency period, high outbreak rate, good genetic stability, and a wide adaptability to temperature and pH. Lysis spectrum experiments show that this phage has broad-spectrum lytic activity against various Vibrio species, including *Vibrio alginolyticus* and *Vibrio parahaemolyticus*, with an overall lysis rate of 80.6%. Animal experiments have demonstrated that this phage significantly improves the survival rate of abalone infected with *Vibrio alginolyticus*, outperforming antibiotics and fish-derived phages. The phage and its composition provided by this invention can be used for the prevention and treatment of vibriosis in aquatic animals such as abalone, offering a highly efficient and environmentally friendly biological control solution as an alternative to antibiotics.

The application discloses a method for identifying pathogenic vibrio based on multiple LAMP technology and belongs to the technical field of pathogen detection. The application overcomes the problem of mutual interference of multiple amplification primers, and a group of primer probe combinations suitable for a five-fold LAMP amplification system are screened, which can simultaneously identify Vibrio mimicus, Vibrio fluvialis, Vibrio alginolyticus, Vibrio metschnikovii and Vibrio furnissii. Further, the core detection reagent is subjected to freeze-drying treatment, is easy to store and transport, has high commercialization degree, and is convenient for primary or on-site detection. The technical scheme provided by the application has important significance for preventing and controlling the outbreak of pathogenic vibrio infection, tracing the source of epidemic diseases and public health monitoring.

This invention discloses a broad-spectrum short-tailed bacteriophage, PhLNJZ2, targeting multidrug-resistant Vibrio and its applications, belonging to the field of microbial technology. The bacteriophage PhLNJZ2 was deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan, China, with accession number CCTCC NO:M 20252251, and classified as follows: Vibrio Phage PhLNJZ2. This bacteriophage was screened from a clinical isolate of a multidrug-resistant Vibrio alginolyticus resistant to more than seven antibiotics. It exhibits highly efficient and broad-spectrum lytic activity against three major aquatic pathogenic Vibrio species: Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio harveyi, with a total lysis rate of 81.25%. This invention also provides compositions containing this bacteriophage, feed additives, and their application in the preparation of drugs or environmental disinfectants for the prevention and control of multidrug-resistant and mixed vibrio infections in aquatic organisms.