Bacillus amyloliquefaciens and application thereof in aquaculture

A technology for dissolving amyloid spores and bacilli is applied in the field of microbial engineering to achieve the effects of improving the ability to resist pathogenic bacteria infection, enhancing non-specific immune function and strong antagonistic effect

Active Publication Date: 2014-12-10
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the wide variety of Bacillus, the characteristics (such as the ability to secrete extracellular enzymes, t

Method used

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  • Bacillus amyloliquefaciens and application thereof in aquaculture
  • Bacillus amyloliquefaciens and application thereof in aquaculture

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Bacillus amyloliquefaciens SIP0902

[0024] Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) SIP0902 was deposited in China Center for Type Culture Collection (CCTCC) on July 4, 2014, the address is Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: M 2014323.

[0025] The strain information is as follows:

[0026] 1. Source: Bacillus amyloliquefaciens SIP0902 was isolated from the intestinal tract of healthy Litopenaeus vannamei, and it was proved to be non-pathogenic to Litopenaeus vannamei by intramuscular injection experiments.

[0027] 2. Morphological characteristics of Bacillus amyloliquefaciens SIP0902:

[0028] Colony characteristics: In TSA medium, the colonies are flat, round, with neat edges, off-white surface, dry, opaque, with a typical white circle in the middle. Cannot grow in TCBS medium.

[0029] Cell features: Gram-positive, short rod-shaped, non-capsulated, mesospore-forming, with a size of 0.6-0.7 μm×0.8-...

Embodiment 2

[0036] The preparation of embodiment 2 bacillus amyloliquefaciens SIP0902 bacterium powder

[0037] Proceed as follows:

[0038] (1) Streak the Bacillus amyloliquefaciens SIP0902 strain stored at -80°C on the seed solid medium;

[0039] (2) After culturing at a constant temperature of 37°C for 24 hours, pick a single colony from the plate and inoculate it into the fermentation broth of Bacillus amyloliquefaciens for fermentation, and the culture temperature is controlled at 28-37°C;

[0040] (3) When the OD600 value of the fermentation broth reaches 1.5-2.0, stop the fermentation; take the fermentation broth and centrifuge, discard the supernatant to obtain wet bacteria sludge, and add 5% trehalose to the bacteria sludge as a freeze-drying protective agent, mix well Freeze-drying to obtain Bacillus amyloliquefaciens SIP0902 powder, wherein the total number of spores of Bacillus amyloliquefaciens SIP0902 is greater than 10 11 CFU / g.

[0041] The mass percentages of the so...

Embodiment 3

[0043] Example 3 Bacillus amyloliquefaciens SIP0902 to the in vitro antibacterial effect of common pathogenic bacteria

[0044] The specific experimental method is as follows:

[0045] 1. Preparation of SIP0902 bacterial suspension and filtrate: Take 5g of SIP0902 sample, add 50ml of sterilized normal saline, shake fully and place in 80°C water bath for 10min; take 10ml of the upper liquid, inoculate into 50ml of sterilized TSB liquid medium, shake at constant temperature Bed shaking culture for 18 hours (cultivation temperature 30°C, rotation speed 140rpm); take 5ml of the above bacterial suspension and transfer it into 50ml of newly prepared TSB liquid medium for 18h at a constant temperature shaking table (culture temperature 30°C, rotation speed 140rpm); take the above A certain amount of bacterial suspension was centrifuged (5000rpm, 10min) to take the supernatant, and the supernatant was filtered with a 0.22μm filter membrane to obtain the SIP0902 filtrate, which was p...

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Abstract

The present invention discloses bacillus amyloliquefaciens and application thereof in aquaculture. The bacillus amyloliquefaciens SIP0902 strain is preserved in the China Center For Type Culture Collection (CCTCC), AND the preservation number is CCTCC NO:M2014323. The bacillus amyloliquefaciens is separated from intestinal tract of penaeus vanmamei, is an endogenous strain, and has strong antagonism to vibrio parahaemolyticus, vibrio anguillarum, vibrio alginolyticus and other 7 kinds of aquatic animal pathogenic vibrios. Bacillus amyloliquefaciens powder prepared from the bacillus amyloliquefaciens can significantly enhance non-characteristic immune function of the penaeus vanmamei, and can improve the pathogen infection resisting ability of prawn.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a strain of bacillus amyloliquefaciens and its application in aquaculture. Background technique [0002] Aquaculture plays a pivotal role in my country's agricultural economy. However, with the rapid development of high-density farming models such as intensification, scale, and factoryization, and the continuous deterioration of the entire farming environment, frequent outbreaks of aquaculture diseases have caused huge economic losses to aquaculture every year; It has played an important role in aquaculture production and is the first choice for people to prevent aquatic animal diseases; however, the increasing resistance of pathogenic microorganisms and people's concern about human health issues such as antibiotic abuse and aquatic product drug residues have forced antibiotics and some chemicals to The application of drugs in aquaculture will be strict...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C02F3/34C12R1/07
Inventor 王芸林黑着牛津王珺陈素文周传朋
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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