Bacillus amyloliquefaciens and application thereof in aquaculture
A technology for dissolving amyloid spores and bacilli is applied in the field of microbial engineering to achieve the effects of improving the ability to resist pathogenic bacteria infection, enhancing non-specific immune function and strong antagonistic effect
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Embodiment 1
[0023] Embodiment 1 Bacillus amyloliquefaciens SIP0902
[0024] Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) SIP0902 was deposited in China Center for Type Culture Collection (CCTCC) on July 4, 2014, the address is Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: M 2014323.
[0025] The strain information is as follows:
[0026] 1. Source: Bacillus amyloliquefaciens SIP0902 was isolated from the intestinal tract of healthy Litopenaeus vannamei, and it was proved to be non-pathogenic to Litopenaeus vannamei by intramuscular injection experiments.
[0027] 2. Morphological characteristics of Bacillus amyloliquefaciens SIP0902:
[0028] Colony characteristics: In TSA medium, the colonies are flat, round, with neat edges, off-white surface, dry, opaque, with a typical white circle in the middle. Cannot grow in TCBS medium.
[0029] Cell features: Gram-positive, short rod-shaped, non-capsulated, mesospore-forming, with a size of 0.6-0.7 μm×0.8-...
Embodiment 2
[0036] The preparation of embodiment 2 bacillus amyloliquefaciens SIP0902 bacterium powder
[0037] Proceed as follows:
[0038] (1) Streak the Bacillus amyloliquefaciens SIP0902 strain stored at -80°C on the seed solid medium;
[0039] (2) After culturing at a constant temperature of 37°C for 24 hours, pick a single colony from the plate and inoculate it into the fermentation broth of Bacillus amyloliquefaciens for fermentation, and the culture temperature is controlled at 28-37°C;
[0040] (3) When the OD600 value of the fermentation broth reaches 1.5-2.0, stop the fermentation; take the fermentation broth and centrifuge, discard the supernatant to obtain wet bacteria sludge, and add 5% trehalose to the bacteria sludge as a freeze-drying protective agent, mix well Freeze-drying to obtain Bacillus amyloliquefaciens SIP0902 powder, wherein the total number of spores of Bacillus amyloliquefaciens SIP0902 is greater than 10 11 CFU / g.
[0041] The mass percentages of the so...
Embodiment 3
[0043] Example 3 Bacillus amyloliquefaciens SIP0902 to the in vitro antibacterial effect of common pathogenic bacteria
[0044] The specific experimental method is as follows:
[0045] 1. Preparation of SIP0902 bacterial suspension and filtrate: Take 5g of SIP0902 sample, add 50ml of sterilized normal saline, shake fully and place in 80°C water bath for 10min; take 10ml of the upper liquid, inoculate into 50ml of sterilized TSB liquid medium, shake at constant temperature Bed shaking culture for 18 hours (cultivation temperature 30°C, rotation speed 140rpm); take 5ml of the above bacterial suspension and transfer it into 50ml of newly prepared TSB liquid medium for 18h at a constant temperature shaking table (culture temperature 30°C, rotation speed 140rpm); take the above A certain amount of bacterial suspension was centrifuged (5000rpm, 10min) to take the supernatant, and the supernatant was filtered with a 0.22μm filter membrane to obtain the SIP0902 filtrate, which was p...
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