13 results about "Litopenaeus" patented technology

This invention discloses a zinc chelate peptide derived from Litopenaeus vannamei, its preparation method, and its application, belonging to the field of polypeptide biotechnology. The process includes: taking Litopenaeus vannamei meat pulp, enzymatically hydrolyzing it with a neutral protease, chelating the hydrolysate with a zinc source, separating and purifying it by gel chromatography, and collecting the highly chelating active component; identifying the highly chelating active component by LC-MS / MS, using water solubility, non-toxicity, low allergenicity, and high zinc chelation potential as screening criteria, and calculating the peptide and Zn content using molecular docking. 2+ By determining the binding energy, highly active zinc chelating peptides were obtained through screening. Five high-binding-energy peptides were identified, three of which can stably chelate zinc: TGPDPDPTEFL, GEIPDSDLPDRRG, and AFDGDGDRNM. The zinc chelating peptides obtained in this invention exhibit high solubility and high cellular safety, significantly promoting zinc transport within cells. They can be used to prepare nutritional fortifiers, oral zinc supplements, or special medical foods.

This invention provides a method for preparing a visual sensor based on mulberry anthocyanins and its application in aquatic product monitoring. Using mulberry anthocyanins as an indicator, combined with citric acid, chitosan, and polyvinyl alcohol, a novel freshness sensor is constructed on filter paper via an impregnation method. The anthocyanins (MAs) exhibit a significant color change within the pH range of 3–12, and their main components are various cyanidin and pelargonidin glycosides. Testing showed that the sensor prepared by the membrane method exhibited the best stability, remaining viable for over 15 days at 4°C, and demonstrating high sensitivity to volatile ammonia. Specifically, the sensor with 1.25% MAs added showed a sensitivity of 11.79%–18.35% to 0.025%–0.1% NH3 within 30 minutes. When used for monitoring the freshness of Litopenaeus vannamei, the sensor's color difference value (ΔE) was highly correlated with storage time and TVB-N value, with the color gradually changing from purplish-red to grayish-blue, achieving visual and qualitative detection of aquatic product freshness.

This invention relates to the field of aquaculture technology, and in particular to a biological attractant based on Schizochytrium fermentation broth and its preparation method; the biological attractant based on Schizochytrium fermentation broth is obtained by processing the fermentation broth after oil extraction from Schizochytrium; the fermentation broth after oil extraction from Schizochytrium is the residual fermentation broth after supercritical CO2 oil extraction or enzymatic hydrolysis oil extraction from Schizochytrium; the preparation method includes: pretreatment of fermentation broth, sterilization and stabilization treatment, concentration and regulation, and filling and storage; the three core components in the fermentation broth work synergistically, umami amino acids quickly trigger the taste perception of aquatic animals, algal polysaccharides slowly release odor molecules to prolong the feeding attraction time, and DHA enhances natural tropism, significantly improving the feeding induction rate of Litopenaeus vannamei, and maintaining a stable feeding attraction effect even in rainy and low-temperature environments; relying on the natural viscosity and structural characteristics of algal polysaccharides, there is no need to add stabilizers such as xanthan gum.

This invention provides a method for treating wastewater from Litopenaeus vannamei farming, comprising the following steps: S1, primary filtration of the farming wastewater, followed by aeration with the addition of compound bacteria to the filtered wastewater, controlling the aeration temperature at 30-35℃, dissolved oxygen at 5-10 mg / L, and treatment time at 10-16 h to obtain aerated wastewater; S2, mixing the aerated wastewater with composite activated carbon and treating it at 30-40℃ and ultrasonic power at 300-500 W for 2-3 h, then filtering to obtain filtrate A; S3, circulating filtrate A through a mixed macroporous adsorption resin at a loading flow rate of (3-5) BV / h to obtain filtrate B, followed by sterilization, thus completing the treatment of Litopenaeus vannamei farming wastewater. This invention effectively purifies Litopenaeus vannamei farming wastewater by adding compound bacteria for aeration treatment, combined with activated carbon and macroporous adsorption resin. This effectively reduces the content of pollutants such as total nitrogen, total phosphorus, and suspended solids in the wastewater, resulting in good treatment effect.

This invention discloses a method for preparing engineered outer membrane vesicles using enzymatic ultrasound technology. The method involves inoculating *E. coli* into LB medium and culturing for 12-16 hours, followed by centrifugation to collect the bacterial cells. The collected bacterial cells are resuspended in lysis buffer and then sonicated for 30-60 minutes to obtain a bacterial solution. After centrifugation, the supernatant is incubated for 30 minutes, followed by centrifugation to separate the precipitate, which is then resuspended in PBS buffer of the same volume as the lysis buffer to obtain a solution containing engineered bacterial outer membrane vesicles. The lysis buffer is prepared by dissolving sorbitol, sodium chloride, EDTA, and lysozyme in Tris-HCl buffer at pH 7.4. This method of preparing engineered outer membrane vesicles using non-genetically engineered strains solves the problems of complex preparation processes, high costs, and inconsistent product quality in existing methods. The obtained engineered outer membrane vesicles can be used as medical preparations for the development of agents to control sporozoites in the Litopenaeus vannamei aquaculture industry.

This invention discloses a method for predicting the dosage of potassium persulfate compound salt in the factory farming of Litopenaeus vannamei, belonging to the field of intelligent disinfection control in aquaculture. The method includes: acquiring the biomechanical parameters of potassium persulfate compound salt's inactivation of pathogenic Vibrio, reactive oxygen species attenuation parameters, Vibrio growth kinetic parameters, and shrimp safety threshold; constructing a microbial growth sub-model, a disinfectant inactivation sub-model, and a disinfectant concentration attenuation sub-model, respectively; and dynamically coupling these three sub-models into a dosage prediction model in system dynamics software. Real-time physicochemical parameters of the aquaculture water are input into the model, and the initial dosage concentration of potassium persulfate compound salt, effective sterilization duration, pathogen regrowth warning, and dosing interval are dynamically output. This invention can dynamically and accurately predict the disinfectant dosage based on the water quality parameters at the aquaculture site, achieving efficient Vibrio control while ensuring shrimp safety, and is suitable for intelligent disinfection management in factory shrimp farming.

This utility model relates to the field of aquaculture technology, and provides an aquaculture transport box, which includes a box body, a partition net fixedly connected inside the box body, a scraping component installed inside the box body, a guide block fixedly connected to the bottom of the box body, and collection boxes inserted and fixedly attached to both sides of the box body. This technical solution solves the problem in the prior art where shrimp larvae produce feces during transportation, which accumulates at the bottom of the box body and pollutes the water after prolonged soaking, thus affecting the survival rate of the shrimp larvae. Furthermore, when transporting Litopenaeus vannamei shrimp, different shrimp larvae need to be classified according to size and packed into different transport boxes. However, because the transport boxes cannot be connected and fixed together, they cannot be stably stacked during transportation and are prone to shaking and impact when subjected to bumps.

This invention belongs to the field of shrimp farming technology, specifically relating to a method for raising Litopenaeus vannamei larvae using bioflocs. The method includes the following steps: (1) preparing raceway-shaped bioflocs; (2) preparing functional bioflocs; and (3) raising mysids and larvae; and raising adult larvae. This invention provides an effective method for raising Litopenaeus vannamei larvae without the use of antibiotics, solving the problem of antibiotic abuse during the larval stage, and is of great significance for promoting the green and healthy development of the shrimp larval industry.

The present invention relates to an intensive photoheterotrophic culture process in high salinity for crustaceans, specifically white shrimp Litopenaeus vannamei. The system allows for better production control by reducing water exchange and discharges into the environment, thus controlling the introduction of harmful agents (pathogens, toxins, predators) and enabling shrimp farming in extreme (desert) regions, where the salinity of the culture system increases significantly due to temperature, solar radiation, and winds, and where there is a lack of fresh water.

The present invention relates to a feed composition suitable for feeding crustaceans comprising mannose oligosaccharides. The invention also relates to the use of mannose oligosaccharide compositions for feeding crustaceans. The invention also relates to crustaceans as a food source, in particular shrimp of the species Litopenaeus vannamei or Penaeus monodon, fed with the feed composition comprising mannose oligosaccharides.

This invention provides a method for cultivating Litopenaeus vannamei suitable for saline-alkali land aquaculture, relating to the field of aquaculture technology. This method for cultivating Litopenaeus vannamei suitable for saline-alkali land aquaculture optimizes the water's calcium content through a customized composite ion-regulating solution. 2+ :Mg 2+ :K + The ratio is 2.8:1:0.3 to control SO4. 2‑ / Cl ‑ The pH was stabilized to 8.2–8.8 using a citrate-sodium citrate buffer system within the range of 0.14–0.8. A "slow-medium-fast" staged acclimatization strategy was employed to adapt to different developmental stages of the seedlings. A combination of Clostridium ethanolate protein (CAP) and compound probiotics was added to the feed to enhance immune metabolic regulation. Experimental results showed that this method improved the survival rate of Litopenaeus vannamei seedlings to 86.3% ± 2.1%, shortened the rearing cycle by 12–15 days, increased hepatopancreatic SOD activity by 42.6%, and achieved a Vibrio inhibition rate of 78.5%, significantly outperforming existing technologies. This invention requires no large-scale pond modification, is adaptable to different types of saline-alkali water bodies, and enables efficient and green aquaculture, possessing significant application value.

The application discloses a BHMT gene molecular marker related to Litopenaeus vannamei resistance to hepatopancreatic intestinal coccidiosis and a detection primer and application thereof, and has the characteristics that the molecular marker comprises three SNP sites on the BHMT gene; the nucleotide sequence of the molecular marker A is shown in SEQ ID NO. 1, the mutation type at the 252th base is T>A, the nucleotide sequence of the molecular marker B is shown in SEQ ID NO. 2, the mutation type at the 93th base is C>T, and the nucleotide sequence of the molecular marker C is shown in SEQ ID NO. 2, and the mutation type at the 636th base is G>A; the parent shrimp with the TA genotype of the BHMT-8159 site, the CT genotype of the BHMT-12167 site and the GG genotype of the BHMT-12710 site is selected as the parent shrimp resistant to hepatopancreatic intestinal coccidiosis; and the application has the advantages of good breeding efficiency and accuracy.