467 results about "Litopenaeus" patented technology

The invention relates to aquaculture feed, in particular to litopenaeus vannamei feed without fish meal. The feed comprises an animal protein source, a plant protein source, a fat source raw material, a sugar source raw material, composite vitamin, composite mineral salt, Chinese medicine compound preparation, premix and additives. The feed has the advantages as follows: 1,palatability, ingestionspeed, growth speed and digestibility are high, the soluble loss of the feed in water is reduced, and mature time is short; 2, the amino acid is balanced, the nutritional requirement of the litopenaeus vannamei on the amino acid is met, the synthesis of the protein by the litopenaeus vannamei is effectively promoted, and discharge of the nitrogen is reduced; 3, disease resistance and survival rate are high and the culture benefit is obvious; 4, absorption and utilization rate is increased, discharge of phosphorous is effectively reduced and pollution to water is reduced; 5, the feed does not comprise artificial color and chemical additives and does not generate adverse influence on the nutritional value of the litopenaeus vannamei; and 6, dependence on the fish meal is reduced and the feeding cost is reduced.

The invention discloses a feed for litopenaeus vannamei, which comprises the following nutrition compositions by mass percent: 25-35% of proteins, 4-9% of fat, 7-12% of ash contents, 40-55% of carbohydrates and 3-11% of water. The feed can meet the nutrient requirement of litopenaeus vannamei for best growth, and effectively solves the traditional problems of high protein level of a compound feedand severe environmental pollution in the process of culturing litopenaeus vannamei; the industrial profit is increased, and the pollution and the damage to a water environment in the process of culturing are reduced; and the sustainable development of litopenaeus vannamei culturing industry is realized.

The invention relates to aquaculture feed, in particular to litopenaeus vannamei compound feed special for winter shed cultivation and a preparation method of the feed. The feed comprises a protein source raw material, a fat source raw material, a sugar source raw material, an antioxidant, a phagostimulant, composite vitamin and composite mineral salt. The invention provides the litopenaeus vannamei compound feed special for winter shed cultivation and the preparation method of the feed, with the advantages of high survival rate, high growth speed, high stability in water, high ingestion speed, convenience for use and low environmental pollution, so as to solve the problems of low growth speed, serious disease, low survival rate and the like of the litopenaeus vannamei during winter shed cultivation at low temperature and promote the healthy development of the litopenaeus vannamei cultivation industry.

The invention relates to an ecological breeding method for mixedly breeding tilapia mossambica and litopenaeus vannamei boon, which is used for breeding fish and prawn in a pond. The invention discloses an ecological breeding mode for mixedly breeding the tilapia mossambica and the litopenaeus vannamei boon. In every spring (from late March to early April), when water temperature stably recoveries at more than 20 DEG C, the pond can be sterilized by calcium oxide in a drying way with quantity being 75-100 kilogram for each acre, after 5-7 days when toxicity is unavailable, the litopenaeus vannamei boon larvaes and the tilapia mossambica seeds are stocked by means of mixed breeding, wherein the litopenaeus vannamei boon is stocked at least for one time. Feeding quantity in each day is depended on 3-5% of the weight of the tilapia mossambica twice a day, and can be properly increased or decreased according to weather, water quality and feeding condition without special feed for the litopenaeus vannamei boon, the pond is sterilized by calcium oxide with quantity being 15-20 kilogram for each acre, once to twice in each month, and the pH value of the pond is adjusted to be alkalescence. The method can naturally obtain the best ecological benefit of cultural environment, reduces disease of fish and prawn, increases physique, improves growth speed, enlarges the individual size, and increases average yield.

The present invention discloses a Fenneropenaeus shrimp low fish meal feed, consisting of the components and the weight percentages that: 15 percent to 23 percent of fish meal, 0 percent to 6 percent of barmy beam pulp, 23 percent to 30 percent of beam pulp, 10 percent to 24 percent of peanut meal, 4 percent to 5percent of beer yeast, 6 percent to 7 percent of shrimp meal, 0 percent to 4 percent of squid meal, 0.2 percent to 0.5 percent of lysine sulphate, 0.09 percent to 0.4 percent of hydroxylv methionine calcium, 0 percent to 0.11 percent of L- threonine, 1 percent to 1.25 percent of fish oil, 0 percent to 1 percent of corn oil, 0 percent to 1 percent of bean oil, 1.5 percent to 2 percent of soyabean lecithin, 17 percent to 23 percent of flour, 0.1 percent of vitamin C phosphate ester, 0.5 percent of choline chloride, 0.2 percent to 0.5 percent of multidimensional premix, 1 percent of phosphate and 0.5 percent to 1 percent of polymineral premix. The feed can not only reduce the use of the fish meal, but also have no bad influence to the growth of the Fenneropenaeus shrimp and the feed efficiency under the comparatively low protein level, thus reducing the feed cost and decreasing the environment pollution in the process of breeding.

The invention relates to a lLitopenaeus vannamei compound feed and belongs to the field of shrimp feed and preparation methods thereof. According to weight percentage, the compound feed contains 10-15percent of fish meal, 18-22 percent of fermented soybean meal, 8-12 percent of peanut cake, 2-6 percent of corn protein powder, 8-10 percent of soya bean meal, 20-25 percent of high gluten flour, 2-6percent of shrimp shell powder, 1-3 percent of selenium-enriched yeast, 1-3 percent of bentonite, 2-4 percent of squid extract, 2-4 percent of kelp powder, 0.5-1.5 percent of monocalcium phosphate, 0.5-1.5 percent of fish oil and 2-4 percent of premix. The compound feed can substitute for the present prawn compound feed and be used for the artificial breeding of litopenaeus vannamei. The compoundfeed is good in palatability, easy to digest and absorb and nice in the stability in water, reduces feed coefficient and cost, nitrogen and phosphorus emission and pollution to the water body environment of culture zones, and can promote the growth of prawn, with balanced nutrition.

The invention belongs to the field of aquaculture or feed, relates to a aquaculture feed, and especially relates to an alga immunopotentiation compound feed for litopenaeus vannamei. The invention at first provides an immunity strengthening agent for litopenaeus vannamei feed, and the agent is obtained through enzymatic hydrolysis and fermentation of an alga composite. The alga composite is composed of following components in parts by dried weight: 40 to 50 parts of asparagus, 15 to 25 parts of enteromorpha, 10 to 20 parts of ulva lactuca, 5 to 15 parts of laver and 5 to 15 parts of undaria pinnatifida. The invention further prepares a compound feed for litopenaeus vannamei by utilizing the immunity strengthening agent. The alga immunity strengthening agent contains algae polysaccharide, which has the effect of strengthening animal immunity, acrylic acid, terpenes, brominated phenolic and some sulfocompounds, which have the antiseptic effect, and sulphated polysaccharide, which has the antivirus effect. The litopenaeus vannameis fed on the alga immunopotentiation compound feed have a strong anti-stress performance, the body's immunity ability is improved, survival rate is high and culture benefit is remarkable.

The invention discloses a low-protein low-fish meal feed for Litopenaeus vannamei. The content of proteins in a Litopenaeus vannamei feed is reduced by supplementing exogenous methionine, the content of fish meal in the feed is reduced by utilizing bean pulp with plant protein sources and peanut meal, and then the exogenous methionine, the bean pulp and plant protein sources and other raw materials are mixed according to a reasonable proportion to form the low-protein low-fish meal feed for the Litopenaeus vannamei. The using amount of the fish meal in the feed is reduced to be less than 10 percent to the maximum extent, and the problem that amino acid is unbalanced in a single protein source is solved by adding a plurality of kinds of plant protein sources. The content of the fish meal and the content of the proteins in the Litopenaeus vannamei feed are remarkably reduced, and the feed is favorable for reducing the cost, improves the utilization rate of the feed, and reduces the discharge of nitrogen and phosphorus in the aquaculture process so as to reduce environmental pollution. Through feeding experiments for a long time, the results show that when the Litopenaeus vannamei is fed by using the low-protein low-fish meal feed for the Litopenaeus vannamei, the feed has no adverse effect on the growth performance and feed coefficient of the Litopenaeus vannamei, and the discharge amount of nitrogen and phosphorus in water bodies can also be reduced.

The invention discloses functional compound feed special for greenhouse cultivation of litopenaeus vannamei. The feed is prepared from import fishmeal, peanut meal, fermented peanut meal, bean pulp, fermented bean pulp, yeast extract, cuttlefish meal, shrimp shell meal, high protein flour, monocalcium phosphate, granulesten, fish oil, a vitaminized mineral mixture premix compound and a functional additive. The functional additive is prepared from vitamins C, bacillus subtilis, seaweed ultrafine smashing micro powder and natural astaxanthin according to proportion. The feed effectively solves the problems of stress, immunity reduction, variety quality losses and the like caused by greenhouse large-scale intensive cultivation of the litopenaeus vannamei and meanwhile has the advantages of being balanced in nutrition, low in cost, green, environmentally-friendly and the like. Main umami substances in muscles of the litopenaeus vannamei fed through the feed are obviously improved, the total amino acid, necessary total essential amino acid and the like are improved in a certain extent, indexes such as the muscle strength, the freshness and the taste are obviously improved, the shrimp body color is close to that of wild shrimps, the immunity of juvenile shrimps and adult shrimps is obviously improved, and the survival rate is high. The feed is suitable for being used in greenhouse high-density large-scale cultivation of the litopenaeus vannamei.

The present invention belongs to the field of aquaculture feed technology, in particular, it relates to an additive premixed material for low-salinity culture of Fanna prawn. Said additive premixed material is composed of vitamin complex and compound mineral matter. Said additive premixed material can be added into prawn feed, and said feed can raise immunity of prawn, and can promote quick growth of prawn, its conversion rate is high and its feed coefficient is 0.9-1.3.

The invention relates to an aquaculture system, in particular to a high-density litopenaeus vannamei ecological engineered healthy breading system. An ecological engineered litopenaeus vannamei breeding facility system is characterized in that: a plastic-covered tunnel (5) which takes a metal rod as a bracket and is covered by a plastic film is arranged above a breeding pool (7), a biological cleaning pool (1), and a biochemical treating pool (2); the breeding pool (7) is spaced by water guide walls (6) in parallel arrangement to form an S-shaped annular flow channel; an outlet of the annular flow channel is communicated with inlets of the biological cleaning pool (1) and the biochemical treating pool (2); the biological cleaning pool (1) and the biochemical treating pool (2) can be opened at the same time, so that water can flow through the biological cleaning pool (1) and the biochemical treating pool (2); or one of the two is opened, and the water just flows through one of the two, and the flow rate of the two is adjustable; the outlets of the biological cleaning pool (1) and the biochemical treating pool (2) are communicated with an air-floating purifying device (3); and an outlet of the air-floating purifying device (3) is communicated with an inlet of the annular flow channel of the breeding pool (7) through a flow device (4). The system provides a good environment and a good condition for the litopenaeus vannamei, saves water resource and energy resource, and is also environmentally-friendly.

The invention relates to an ecological circulation system and a breeding method, in particular to an ecological circulation system for litopenaeus vannamei systematic breeding and a breeding method. The ecological circulation system comprises a litopenaeus vannamei pond, a net frame aerating system is arranged in the litopenaeus vannamei pond, a sewage suction and energy-saving discharge system is arranged at the bottom of the litopenaeus vannamei pond and communicated with a sewage suction and filter system, the sewage suction and filter system is communicated with a water circulating and refluxing system, and the water circulating and refluxing system is communicated with the litopenaeus vannamei pond. The breeding method includes steps of pond cleaning and treating, litopenaeus vannamei and bait throwing, periodically cleaning, water circulating and sewage suction, aerating equipment starting, pond patrolling and observing and litopenaeus vannamei outputting. The ecological circulation system and the breeding method have the advantages of compact and reasonable structure, comprehensive circulation and utilization, breeding cost reduction and higher litopenaeus vannamei quality.

The invention discloses a pond poly-culture method for tilapia mossambica and litopenaeus vannamei. The pond poly-culture method comprises the following steps of: adding quick lime into a dry pond in spring every year, injecting water to carry out water sterilization, adding 50 kg-100 kg of fermented chicken manure into each mu of the pond when the water temperature is stabilized to over 20 DEG C, injecting water until water depth is 40cm-60cm, waiting for 1-2 weeks, adding 8000-12000 litopenaeus vannamei shrimp seeds into each mu of the pond, and feeding special poly-culture feed; injecting water after 15-20 days until water depth is 100cm-120cm, adding 1500-2500 tilapia mossambica fries into each mu of the pond, feeding the poly-culture feed, injecting water after 15-20 days until water depth is 200cm-250cm, oxygenating for 3-7 hours every day, and mixing 20kg-30kg of the quick lime with water to spray for each mu of the pond once to twice every month; catching the tilapia mossambica and litopenaeus vannamei after 120-150 days. According to the pond poly-culture method disclosed by the invention, the special poly-culture feed is fed, prawn quality is high, water pollution is less, management is easy, cost is low and benefits are high.

The invention relates to a compound immunopotentiator for post larvae of litopenaeus vannamei, which comprises the following components in percentage by weight: 20-30 percent of beta-glucan, 20-30 percent of chitosan, 5-10 percent of ginsenoside, 10-20 percent of astragalus polysaccharides, 5-10 percent of phospholipid, 40-60 percent of vitamin C-2-polyphosphate ester, 10-15 percent of vitamin E, 5-10 percent of vitamin A, 10-20 percent of bacillus subtilis and 1-5 percent of clamworm powder. The invention has the following advantages that: (1) the problem of feedback inhibition of the post larvae of litopenaeus vannamei is overcome effectively; (2) the immunity of the post larvae of litopenaeus vannamei is enhanced obviously, and the survival rate is increased; (3) the activity and the growth speed of the post larvae of litopenaeus vannamei are increased obviously; (4) the compound immunopotentiator has the addition amount of only 0.2-0.3% when used as a feed additive for a particle feed of the post larvae of litopenaeus vannamei; (5) no adverse effect on the palatability of the feed exists; and (6) the source of the raw materials is stable, the cost is low, and the production process is simple.

Disclosed is a mixed feed for improving prawn quality, wherein the mixed feed is prepared from the following raw materials (by weight ratio): fish meal 25-33%, soya bean waste 18-22%, maize protein meal 8-10%, refined flour 20-25%, brewers' yeast 3-5%, selenium-rich yeast 2-3%, squid grease 3-4%, sea-tangle powder 2-3%, monobasic calcium phosphate 0.8-1%, fish oil 0.8-1.2%, vitamin premixed material 1-2%, mineral premixed material 1-2%, astaxanthin 0.2-0.5g, carnitine 0.2-0.3g, Saccharicter-penin 50-70mg, soybean isoflavones 20-30mg, VC 1-2g, and choline chloride 1-2g.

The invention relates to compound feed with immunity-enhancing Chinese herbal medicine for South America white shrimps. The compound feed is characterized by comprising the following raw materials by weight percentage: 30 to 35 percent of fish meal, 2 percent of beer yeast, 3 percent of corn protein powder, 3 percent of shrimp shell meal, 2 percent of blood meal, 3 percent of cuttlefish meal, 10 to 13 percent of bean pulp, 12 to 16 percent of peanut cake, 22 to 24 percent of wheat flour, 2 percent of kelp powder, 2 to 3 percent of soybean phosphatide, 1 to 2 percent of fish oil and 2 percent of premix compound for the South America white shrimps. The compound feed meets the industrial standards and requirements for growth of the shrimps, has a bait coefficient less than 1.0 for culture in a pond with high water level, and can improve immunity and disease resistance of the shrimps, prevent the shrimps from infecting kwashiorkor and achieve the survival rate of more than 80 percent in culture process. Based on a prevention-oriented culture, the compound feed can reduce diseases and use of chemical drugs and antibiotics for culture of the shrimps, and helps to provide customers with green drug residue-free shrimp products.

The invention relates to a culture method for small and medium-sized litopenaeus vannamei and belongs to the technical field of aquaculture. The culture method specifically comprises the following eight steps of: pond clearing, water introduction, disinfection, bait organism culture, water quality detection, staged shrimp seed putting, staged culture, and small and medium-sized catching and harvesting. The method adopts strict treatment such as secondary sedimentation, disinfection and secondary filtration for water for culture at each stage, one-time water introduction and sealed culture to effectively isolate the invasion of external pathogens, and does not use drugs during the whole culture process, so that the product does not have drug residues and has good quality; the staged shrimp seed putting adopts a method of pure-oxygen aeration and dry transportation by putting shrimp seed into a netting cage, with lower shrimp seed damage rate which is generally controlled below 1.0%; and a three-stage culture mode is adopted for culture and can realize 5-generation culture throughout the year, with the average yield of 750-1,000kg / mu, the culture success rate higher than that of conventional culture by 20-30%, and the output value increased by 50% compared with a conventional mode.

The invention discloses a breeding method for fine breeds of the litopenaeus vannamei. The breeding method comprises the steps: (1), introduction of a basic population; (2), screening of the basic population; (3), intensifying of parent shrimps; (4), building of a family; (5), standardized breeding of young shrimps; (6), independent breeding of the family; (7), family testing; (8), determining of a target family; (9), expanding of propagation; (10), colony breeding of the target family; and (11), breed conservation. Compared with the prior art, the breeding method for the fine breeds of the litopenaeus vannamei has the advantages of being high and stable in yield, resistant to stress and suitable for the high-salinity and low-salinity breeding environment of China.

The invention provides a Litopenaeus vannamei base population establishing method based on genetic information and excellent properties. The method comprises the following detail steps that multiple Litopenaeus vannamei populations with different excellent properties are selected, the genetic relationship among introduced individuals is analyzed by using molecular markers, a hybridization scheme is designed based on the genetic relationship information and excellent property information, and a great numbers of families are established to form a Litopenaeus vannamei base population with relative abundant heritable variation and excellent properties. According to the method, in the establishing process of the Litopenaeus vannamei base population, the molecular markers are used for detecting the genetic relationship among different individuals and designing the hybridization scheme, inbreeding can be avoided, genetic linkages can be broken through, sufficient recombination and orientated mating can be acquired, continuity and scientificity of breeding work are guaranteed, thus the acquisition of Litopenaeus vannamei base populations with the relative abundant heritable variation and excellent properties can be facilitated, therefore, genetic improvement and production performance improvement can be further carried out on the later generations of the Litopenaeus vannamei to a large extent, and better economic benefit is brought.

The invention relates to an SNP marker related with low dissolved oxygen survivability of litopenaeus vannamei, screening and applications thereof, and belongs to the technical field of molecular marker assisted selection of aquatic animals. An SNP molecular marker is cloned from SOD gene. The SNP marker is that the R in the 118-th base from the 5' terminal of the nucleotide sequence represented by SEQ ID No.1 is C or T. The SNP marker is prominently related with the character of tolerating low dissolved oxygen of litopenaeus vannamei, and thus can be applied to the molecular marker assisted selection so as to screen litopenaeus vannamei with strong performance of tolerating low dissolved oxygen. The provided SNP marker can be used to carry out early stage screening according to the actual needs, thus the efficiency and accuracy of breeding are effectively improved, the genetic level of litopenaeus vannamei is improved, species with strong stress resistance can be accurately and efficiently bred, the SNP marker is practical and the application range is wide.

The invention discloses a feed for litopenaeus vannamei boone, in particular to a composite feed for feeding litopenaeus vannamei boone cultured at low salinity, which comprises the following components in part by weight: 20 to 30 parts of manhattan fish meal, 10 to 16 parts of bean pulp, 10 to 14 parts of peanut cake, 7 to 10 parts of corn protein powder, 18 to 20.5 parts of high gluten flour, 1to 3 parts of molasses yeast, 1 to 3 parts of squid extract, 0 to 1 part of table salt, 1 to 2 parts of calcium lactate, 0 to 0.5 part of monocalcium phosphate, 1 to 2 parts of fish oil, 1 to 2 partsof soyabean lecithin, 1 part of vitamin premix and 1 part of mineral premix. The feed is mixed simply and balance in nutrition, can obviously improve the growth of the litopenaeus vannamei boone cultured at low salinity and improve the meat quality and color of the litopenaeus vannamei boone cultured at low salinity.

The invention discloses albumen powder richening in antimicrobial peptide fly maggot, a preparation method for the albumen powder and a feed additive of the albumen powder. The albumen powder richening in the antimicrobial peptide fly maggot is prepared by a method comprising the following steps of: (1) feeding flies, luring the flies to lay eggs and feeding larva by using feed after incubation; (2) conducting ultrasound induction on the larva; (3) continuously feeding, collecting the larva, washing, conducting disinfection treatment, and conducting tissue homogenate smashing; and (4) filtering, treating filtrate at 0-5 DEG C, removing surface lipid compositions, drying to obtain the albumen powder; adding auxiliary materials in the albumen powder, pelletizing to obtain the feed additive. A ultrasound stimulation mode under specified conditions is adopted to induce the larva to enable an antimicrobial peptide active ingredient content in a body of a housefly to obviously improve, and the feed additive has certain growth promotion and disease-resistant strengthening effects on litopenaeus vannamei, crucian and the like.

The invention relates to a small pool of clear water-breeding method of litopenaeus vannamei using in aquaculture industry, the method includes three key controlling progresses which are water conditioning control, fed diet controlling and synchronized quality control. The water conditioning progress is supported by that as follows: after the water is sterilized through sand filtration, ozone and ultraviolet, it must be filtered by turns through three different filter membrane the pore diameter of which are respectively 10,1.0,0.5 mum, and in the later period of nursery, 100 000 cell / ml chlorella must be invested into the water in order to control its quality; fed diet controlling is carried out through using bone of algae, algae and chaetoceros harvest worms far bait, investing and feeding artificial synthesized bait during the second stage of zoea larva; synchronized quality control will be synchronized through salinity tolerance test or qualified hunger tolerance that will be carried out after emergence.

The invention discloses a method for deeply refrigerating, quickly freezing and preserving litopenaeus vannamei. The complete process for deeply refrigerating, quickly freezing and preserving litopenaeus vannamei comprises the steps of cleaning, draining, positioning, quickly freezing, rinsing, draining to dryness, packaging and the like. Ice crystals formed by products processed by adopting the method are small, the cell completeness of the products is ensured, the original color, freshness and flavor of the products can be kept, the shelf life of the products is greatly prolonged, the preservation effect is good, the year-round supply requirement can be met, and the method has very good application values.

The invention discloses a litopenaeus vannamei larvae rearing and breeding method. The method comprises the steps of larvae rearing and breeding device assembly and sterilization, larvae rearing and breeding water sterilization, larvae putting and feeding, and larvae rearing water quality control. A breeding device adopted in the method is simple in structure, reasonable in arrangement and convenient and easy to operate and use. Automatic control can be achieved in multiple steps, live algae, probiotics and periodic blow down and water change are adopted for water quality control, ecological safety is guaranteed, the design is reasonable, the water environment for larvae rearing is easier to stabilize, and various diseases are prevented. The litopenaeus vannamei larvae rearing and breeding method is simple and efficient.

The invention relates to a method of cultivating biological flocs and an application thereof in breeding litopenaeus vannamei and relates to cultivation of biological flocs. The cultivated biological flocs comprise bacillus pumilus, lactobacillus acidophilus and pichia guilliermondii. The method comprises the following steps: preparing a bacillus pumilus inoculant, a lactobacillus acidophilus inoculant and pichia guilliermondii powder; adopting a way of splashing bacillus pumilus powder, saccharomycetes powder and a lactic acid bacteria inoculant for a water body per mu every day, and arranging an nano aerating tube in the lower layer around a cultivation pond to aerate to form the flocs; adjusting the C / N weight of a cultivating water body and controlling the C / N at (12-14): 1; and after the biological flocs are cultivated, splashing the bacillus pumilus inoculant, pichia guilliermondii powder and a lactobacillus acidophilus inoculant to the water body per mu every day. The cultivated biological flocs can be applied to breeding litopenaeus vannamei.

The invention relates to a special compound feed for litopenaeus vannamei. The feed is composed of following components in percentage by weight: 18 to 24% of steam fish meal, 17 to 22% of soybean meal, 15 to 24% of peanut meal, 2 to 4% of spray-dried animal blood cells, 2 to 4% of squid paste, 2 to5% of yeast extract, 20 to 25% of flour, 2 to 3% of fish oil, 1 to 2% of soybean oil, 1.0% of shrimp multi-vitamin, 2.0% of shrimp multi-mineral and 1.0% of litopenaeus vannamei special feed addictive, and the sum of the components mentioned above is 100%. The nutrition composite of the compound feed is obtained from numerous tests according to the nutrition need of litopenaeus vannamei, can fully satisfy the nutrition need of litopenaeus vannamei, improves the immunity and disease preventing ability of litopenaeus vannamei, and promotes the healthy growth of litopenaeus vannamei.

The invention relates to an ecological bait for the low salinity ecological cultivation of Lito penaeus vannamei in autumn and winter, which is prepared from the following raw materials (by weight ratio): basic nourishing constituents 85-95%, pharmaceutical constituents 1-5%, enzyme constituents 3-10%. The invention also provides the process for preparing the bait.

The invention belongs to the field of molecular biology DNA marking technology and application, in particular to a detecting method for a Litopenaeus vannamei Boone LvE165 microsatellite DNA marker. The invention provides a Litopenaeus vannamei Boone LvE165 microsatellite specific DNA primer, a PCR (Polymerase Chain Reaction) system utilizing the primer and a detecting method for the Litopenaeus vannamei Boone LvE165 microsatellite DNA marker. The method comprises the following steps of: extracting a Litopenaeus vannamei Boone genome and diluting for later use; designing specific primers at both ends of a sequence by utilizing a Litopenaeus vannamei Boone LvE165 microsatellite DNA core sequence; and then carrying out PCR amplification on genome DNA of different groups of Litopenaeus vannamei Boone or individuals in the group by using the primer, analyzing a product and confirming the genotype of each individual so as to obtain a polymorphism genetic variation map. The invention is mainly applied to Litopenaeus vannamei Boone germplasm resource and genetic diversity analysis, molecular population genetics, construction of the genetic map, and the like.

The present invention provides compound feed for the polyculture of Litopenaeus vannamei and Trachinotus ovatus and a preparation method thereof. The compound feed is characterized by comprising the following ingredients in parts by weight: 15-25 parts of fish meal, 5-10 parts of meat and bone meal, 1-5 parts of maggot powder, 15-25 parts of soybean meal, 5-10 parts of peanut bran, 1-5 parts of soybean protein concentrate, 1-5 parts of cottonseed meal, 1-5 parts of corn gluten meal, 1-3 parts of molasses yeast, 1-3 parts of corn DDGS, 1-3 parts of squid paste, 1-3 parts of shrimp shell powder, 20-25 parts of high-gluten flour, 1-2 parts of sea fish oil, 1-2 parts of soybean oil, 1-2 parts of soy lecithin, 0.5-1 parts of calcium dihydrogen phosphate, 0.1-0.3 parts of choline chloride, 0.1-0.3 parts of cholesterol, 0.05-0.1 parts of L-ascorbate-2-monophosphate, 0.1-0.3 parts of coated lysine, 0.1-0.3 parts of coated methionine, 0.1-0.3 parts of Bacillus subtilis, 0.1-0.3 parts of mannan, 0.1-0.5 parts of complex vitamins, 0.1-0.5 parts of complex mineral salts. Experiments show that the compound feed for the polyculture of Litopenaeus vannamei and Trachinotus ovatus of the present invention can effectively meet the comprehensive nutrient needs of both Litopenaeus vannamei and Trachinotus ovatus, significantly improves the effective utilization of the feed, and has a relatively low food coefficient.