66 results about "Litopenaeus" patented technology

The invention discloses a xanthine oxidase inhibitory peptide with uric acid reducing activity and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is obtained by conducting enzymolysis, separation and purification on processing byproducts such as litopenaeus vannamei heads and shells with xanthine oxidase inhibitory activity. The xanthine oxidase inhibitory peptide is screened by using a molecular docking technology, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Pro-Val-Pro-Pro-Pro. The xanthine oxidase inhibitory peptide has an XOD (X-Oxidase) half inhibitory concentration of 3.181 + / -0.1786 mM. The invention further proves that the xanthine oxidase inhibitory peptide has a certain improvement effect on hyperuricemia mice, and the xanthine oxidase inhibitory peptide mainly has the effects of reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and liver of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and the xanthine oxidase inhibitory peptide has a certain improvement effect on the hyperuricemia mice through Toxinpred prediction. The xanthine oxidase inhibitory peptide uric acid reducing peptide is free of toxic and side effects and has a wide market application prospect.

The invention provides a penaeus vannamei behavior state acoustic intelligent identification method and a model building method, and belongs to the technical field of aquaculture acoustic monitoring. Comprising the following steps: acquiring audio data of penaeus vannamei in different behavior states; filtering and framing, threshold detection and feature extraction are carried out on the audio, and a standardized audio feature data set is constructed; a deep learning model ASP-SEResNet fusing attention statistical pooling and a channel attention mechanism is built, the deep learning model comprises a feature channel optimization branch based on a compression-excitation module and a time sequence feature expression branch adopting attention weighted statistic aggregation, and joint representation of acoustic features is achieved through a double-path feature fusion mechanism; training and optimizing the model to obtain a final model; experimental results show that the method shows excellent classification performance on a prawn audio feature data set, and high-precision identification of five behavior states of the penaeus vannamei can be realized.

The invention provides an anti-white spot syndrome virus peptide derived from litopenaeus vannamei, and belongs to the technical field of biology. The amino acid sequence of the anti-white spot syndrome virus peptide provided by the invention is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.2. The invention further provides a recombinant expression vector and host bacteria containing the anti-white spot syndrome virus peptide gene, and application of the recombinant expression vector and host bacteria in anti-white spot syndrome virus drugs, vaccines, feeds and feed additives, and the recombinant expression vector and host bacteria have wide application prospects and economic benefits.

The application relates to a feed additive of herbal extracts for Litopenaeus vannamei and application thereof. The feed additive of herbal extracts is composed of dandelion extracts and houttuynia cordata extracts. The dandelion extracts and the houttuynia cordata extracts are mixed in a proportion of 1:1 and then added into the basic feed of Litopenaeus vannamei, so that the growth performance of the Litopenaeus vannamei is remarkably improved, the intestinal and hepatopancreas tissue structures are improved, and the immunity is enhanced. Meanwhile, the extracts effectively regulate the intestinal flora structure, increase the abundance and diversity of the intestinal flora, and promote the nutrient absorption and digestion functions. The extracts have a synergistic effect, are low in price, green in environmental protection, safe and free of residues.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The application discloses a SNP molecular marker of Litopenaeus vannamei growth traits, a detection primer, a kit and application thereof. The SNP molecular marker is shown as SEQ ID NO. 1, and the polymorphism of the base at the 497th position from the 5' end of SEQ ID NO. 1 is A / G; the SNP molecular marker can simultaneously screen body length and weight to carry out Litopenaeus vannamei breeding, and the SNP molecular marker can also be detected by using a primer designed according to the sequence of SEQ ID NO. 1 or prepared into a kit. Meanwhile, the AA genotype of the SNP molecular marker Chr25_11409188 provided in the application can be used to select Litopenaeus vannamei parents, further improve the growth speed of the body length and weight of a breeding population, provide an innovative tool for molecular breeding of Litopenaeus vannamei, shorten a breeding cycle, and help industrial development and efficiency improvement.

The application discloses a method for constructing a dynamic specialized line of Litopenaeus vannamei nitrite tolerance traits. The method comprises the following steps: collecting and cultivating a nitrite tolerance population, screening the nitrite tolerance population, screening three rounds of individuals in the nitrite tolerance population, cultivating zero-generation parents and breeding F1 generation, cultivating the F1 generation and breeding F2 generation, cultivating the F2 generation and breeding F3 generation, and dynamically maintaining the nitrite tolerance trait specialized line. The specialized line breeding method of the Litopenaeus vannamei has the characteristics of simplicity, high efficiency and low cost, and does not need to construct a family line, thereby laying a technical foundation for large-scale and extensive breeding of the Litopenaeus vannamei nitrite tolerance traits.

The invention belongs to the technical field of genetic engineering, and particularly relates to a double-stranded RNA preparation for regulating and controlling shrimp molting as well as a synthesis method and application of the double-stranded RNA preparation. The prawn shelling is regulated and controlled by synthesizing specific double-stranded RNA, and the preparation method of the preparation comprises the following steps: firstly, cloning a target gene cDNA sequence related to the key physiological process of prawn shelling; secondly, constructing the sequence into an in-vitro transcription vector, and extracting and purifying recombinant plasmids; and finally, synthesizing the high-purity and high-stability double-stranded RNA preparation aiming at the target gene by taking the plasmid as a template and utilizing an in-vitro transcription system. In-vivo injection experiments prove that when the dsRNA preparation is injected into the litopenaeus vannamei body, the molting process of the litopenaeus vannamei can be obviously delayed.

The application discloses a method for constructing a fast-growing specialized line of Litopenaeus vannamei. The method comprises the following steps: collecting and cultivating a fast-growing population, screening the fast-growing population, screening individuals in the fast-growing population for three rounds, cultivating zero-generation parents and breeding F1 generation, cultivating the F1 generation and breeding F2 generation, cultivating the F2 generation and breeding F3 generation, and dynamically maintaining the fast-growing specialized line. The shrimp line cultivation method of the application does not need to construct a family line, and has the characteristics of simplicity, high efficiency and low cost, thereby laying a technical foundation for large-scale and extensive breeding of the fast-growing trait of Litopenaeus vannamei.

This invention discloses a SNP marker, detection primers, and applications for genes related to nitrite resistance in Litopenaeus vannamei. The SNP molecular marker is located at the 193bp site of the sequence shown in SEQ ID NO.2, with mutation types of A / A homozygous, G / G homozygous, and A / G heterozygous. Litopenaeus vannamei with the A / A homozygous SNP molecular marker exhibits significantly higher nitrite resistance than those with the G / G homozygous and A / G heterozygous genotypes. This invention accelerates the breeding process of superior nitrite-resistant Litopenaeus vannamei varieties by identifying SNP markers associated with nitrite resistance and applying these markers to establish a marker-assisted breeding method for nitrite resistance in Litopenaeus vannamei.

This invention provides a method for the artificial breeding of Litopenaeus vannamei, including steps such as scientifically selecting broodstock, optimizing the breeding environment, controlling the breeding process, fertilization, hatching of fertilized eggs, and intensive rearing. During the breeding process, specific photoperiods and staged feeding strategies are employed, with different ratios of formulated feed and nutritional preparations to meet the nutritional needs of the broodstock. After initial rearing, the hatched juvenile shrimp are graded and selected, followed by intensive rearing. During the intensive rearing stage, water quality parameters are adjusted, and necessary trace elements, vitamins, and specific feeds are administered to promote the healthy growth of the juvenile shrimp. This invention, through meticulous management, significantly improves the breeding success rate and larval survival rate of Litopenaeus vannamei, providing an effective breeding strategy for aquaculture.

This invention provides a method for preparing a visual sensor based on mulberry anthocyanins and its application in aquatic product monitoring. Using mulberry anthocyanins as an indicator, combined with citric acid, chitosan, and polyvinyl alcohol, a novel freshness sensor is constructed on filter paper via an impregnation method. The anthocyanins (MAs) exhibit a significant color change within the pH range of 3–12, and their main components are various cyanidin and pelargonidin glycosides. Testing showed that the sensor prepared by the membrane method exhibited the best stability, remaining viable for over 15 days at 4°C, and demonstrating high sensitivity to volatile ammonia. Specifically, the sensor with 1.25% MAs added showed a sensitivity of 11.79%–18.35% to 0.025%–0.1% NH3 within 30 minutes. When used for monitoring the freshness of Litopenaeus vannamei, the sensor's color difference value (ΔE) was highly correlated with storage time and TVB-N value, with the color gradually changing from purplish-red to grayish-blue, achieving visual and qualitative detection of aquatic product freshness.

The invention discloses a litopenaeus vannamei egg membrane outer layer protein VMO1 and an application thereof in egg entering of a prawn foreign protein. The amino acid sequence of the egg membrane outer layer protein VMO1 of the litopenaeus vannamei is as shown in SEQ ID NO. 2. The litopenaeus vannamei vitelline membrane outer layer protein gene LvVMO1 is cloned from litopenaeus vannamei for the first time, and experiments prove that the litopenaeus vannamei vitelline membrane outer layer protein gene LvVMO1 is generated in the hepatopancreas tissue of the litopenaeus vannamei and enters eggs through a hemolymph way. A VMO1-EGFP in-vivo tracing test further shows that VMO1 is generated in hepatopancreas of the litopenaeus vannamei, enters the ovary through hemolymph, can carry foreign proteins such as EGFP, is transferred from an abdominal injection site and is enriched in the ovary. The invention provides an effective technical means for introducing a gene editing system into litopenaeus vannamei egg cells by using foreign proteins to realize genetic improvement and other applications of litopenaeus vannamei, and has wide application prospects and great economic values.

The invention discloses a litopenaeus vannamei Vg polyclonal antibody as well as a preparation method, a quantitative detection method and application thereof, and relates to the field of biotechnology and immunodetection. The polyclonal antibody is obtained by immunizing a polypeptide antigen containing an amino acid sequence SEQ ID NO: 1 (CPTKYEIETEGEKV), and the polyclonal antibody can specifically recognize the litopenaeus vannamei Vg. The invention provides and establishes a novel non-double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method for the first time, and the core of the novel non-double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method is that a Litopenaeus vannamei VgR ligand binding structural domain is used as a solid-phase capture molecule, and the polyclonal antibody is used as a detection antibody to form a receptor-ligand-antibody detection mode. The method effectively avoids the technical bottleneck that paired antibodies are difficult to prepare in a traditional method, and has the advantages of wide linear range and good repeatability.

The invention relates to the technical field of litopenaeus vannamei feeds, in particular to an artificial compound feed for improving the muscle quality of freshwater cultured litopenaeus vannamei and a preparation method thereof.The artificial compound feed is prepared from, by weight, 18%-22% of fish meal, 33%-37% of soybean meal, 20%-24% of wheat flour, 1%-5% of squid powder, 2%-3% of fish oil, 1%-2% of soybean lecithin, 0.2%-1% of choline chloride and 0.1%-0.2% of calcium propionate; the feed additive is prepared from the following raw materials in percentage by weight: 0.05%-0.1% of an antioxidant, 0.2%-0.5% of sodium alginate, 1%-2% of monocalcium phosphate, 1%-2% of a multi-vitamin multi-mineral premix, 0.8% of a hydroxyproline and glutamine mixture and 10%-13% of microcrystalline cellulose. 0.8% by weight of a mixture of hydroxyproline and glutamine is added, so that the collagen content, hardness, water holding capacity and muscle fiber density in the muscle of the freshwater cultured prawns as well as the content of flavor amino acids (such as glycine, alanine and glutamic acid) and important umami substances IMP are remarkably improved; therefore, the muscle taste and flavor of the low-salt or freshwater cultured litopenaeus vannamei are improved.

This invention provides a molecular marker 27W1 for breeding high-fertility Litopenaeus vannamei populations and its applications. The nucleotide sequence of the molecular marker 27W1 is shown in SEQ ID No. 1, wherein the allele frequency of G at base 301 in high-fertility Litopenaeus vannamei populations is ≥98.33%. This invention also provides primers for the molecular marker 27W1, including amplification primers with nucleotide sequences shown in SEQ ID No. 2 and SEQ ID No. 3, and extension primers with nucleotide sequences shown in SEQ ID No. 4. The high-fertility molecular marker 27W1 for Litopenaeus vannamei can be used for early breeding of Litopenaeus vannamei, regardless of growth stage, to rapidly select parent populations with excellent reproductive traits and promote the breeding process of new high-fertility Litopenaeus vannamei varieties.

The application discloses a SNP molecular marker related to the low-oxygen tolerance trait of Litopenaeus vannamei Lvpsma5 genes, wherein the SNP molecular marker is located at the 904th nucleotide from the 5' end of a nucleotide sequence shown in SEQ ID NO:1, and the base is T or C; the application also discloses primers for amplifying the SNP molecular marker, a kit, a detection method of the low-oxygen tolerance trait of Litopenaeus vannamei, and application of reagents for detecting the SNP molecular marker, the primers, the kit or the method in identifying or breeding the low-oxygen tolerance trait of Litopenaeus vannamei; the low-oxygen tolerance trait of an individual with a TC genotype of the SNP molecular marker is significantly higher than that of an individual with a TT genotype; the SNP molecular marker can be used for molecular marker assisted breeding and genetic improvement of Litopenaeus vannamei, so as to improve the low-oxygen tolerance of Litopenaeus vannamei and increase the breeding benefit.

The invention discloses dsRNA (double-stranded ribonucleic acid) targeting a C3orf38 gene of litopenaeus vannamei and application of the dsRNA. According to the invention, dsRNA targeting the Litopenaeus vannamei C3orf38 gene (LvC3orf38) is designed and synthesized, and the dsRNA is used for inhibiting gene expression, so that it is found that inhibition of LvC3orf38 gene expression can inhibit duplication of the DIV1 in a prawn body, and the survival rate of the prawn infected with the DIV1 is improved. In addition, by inhibiting expression of the LvC3orf38 gene, weight gain of the prawns can be promoted, and growth of the prawns is promoted. Therefore, a preparation capable of inhibiting the expression of the LvC3orf38 gene can be used for improving the resistance of the prawns to the decapod iridovirus disease and promoting the growth of the prawns. A green and environment-friendly prevention and treatment strategy is provided for prevention and treatment of the full-eye iridovirus disease, the contradiction between disease resistance and growth can be solved, and the requirements of the modern breeding industry for high efficiency, safety and economy are met.

The invention relates to the technical field of genetic breeding of aquatic animals, in particular to a low-loss litopenaeus vannamei growth phenotype determination method. The preparation method comprises the following steps: performing anesthesia treatment on a prawn sample to be measured to enable the prawn to enter an anesthetic state, then transferring the prawn into a normal seawater environment, measuring the growth phenotype of the prawn in a window period, and then recovering the normal state of the prawn. According to the method disclosed by the invention, through short-time eugenol anesthesia, mechanical injury and stress reaction caused by jumping and struggling of the prawns in the phenotype determination process are effectively inhibited, and the loss rate of the prawns is obviously reduced. Compared with the prior art, the method has the advantages of simplicity and convenience in operation, extremely low prawn loss, high growth phenotype determination efficiency and the like, is suitable for growth character evaluation in a large-scale genetic breeding process of prawns, and has a wide application prospect.

The application provides a use of a compound BMS309403 in preparation of a medicament for preventing and treating acute hepatopancreas necrosis disease of prawns, and belongs to the technical field of prawn disease prevention and treatment. It is unexpectedly found that an inhibitor of a fatty acid binding protein LvFABP of Litopenaeus vannamei, i.e. the compound BMS309403, can be effectively applied in the prevention and treatment of AHPND, which can not only reduce the disease of hepatopancreas and intestinal tract of prawns, but also effectively reduce the Vibrio virulence gene copy number of hepatopancreas of diseased prawns, so that the survival rate of prawns infected with acute hepatopancreas necrosis disease can be significantly improved. In addition, as a small molecule compound, the BMS309403 is more superior to small molecule double-stranded RNA in synthesis, preservation and use, and therefore can have a better prospect in the actual production application of AHPND prevention and treatment.

The application provides a MNP marker site for identifying a fast-growing Litopenaeus vannamei population, a primer set and application thereof, and belongs to the technical field of molecular genetics of aquatic animals. The MNP marker site, the corresponding primer set and the kit thereof can screen the fast-growing Litopenaeus vannamei population, and it is proved that the MNP marker site can realize high-throughput analysis, avoid a large number of non-existent false genotypes, reduce detection cost and analysis complexity, and the accuracy of identifying the fast-growing Litopenaeus vannamei population is as high as 100%. The MNP marker site and the application thereof provided by the application have reference significance for the field related to molecular genetics and genotype analysis of aquatic animals.

This invention discloses a molecular marker for the Raptor gene of Litopenaeus vannamei, comprising molecular markers D.11926, D.11999, D.12025, D.12026, D.12039, D.12044, D.12063, D.12195, D.12212, D.12241, and D.12349, which are located sequentially at positions 69, 142, 168, 169, 182, 187, 206, 338, 355, 384, and 492 of the nucleotide sequence shown in Sequence 1 of the sequence listing. The molecular markers described above can be used as functional markers for the nitrite tolerance trait in Litopenaeus vannamei and applied in the breeding of Litopenaeus vannamei to cultivate Litopenaeus vannamei strains with good nitrite tolerance.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

The application discloses an antibacterial and antiviral polypeptide Litomusin for white shrimp 81‑100 and application thereof, the molecular formula of the polypeptide is C 106 H 183 N 35 O 25 S1, and the amino acid sequence of the polypeptide is shown as SEQ ID NO. 01. The polypeptide has good water solubility, has significant antibacterial activity on various bacteria and fungi, has good antibacterial effect and high efficiency, has a wide antibacterial spectrum, has strong activity on white spot syndrome virus, has no toxicity on hematopoietic tissue (Hpt) cells of Procambarus clarkii, and has a good application prospect in drug research and development.

The invention provides a litopenaeus vannamei head umami peptide and a preparation method thereof, and the umami peptide is characterized in that the amino acid sequence of the umami peptide is selected from any one of the sequences as shown in SEQ ID NO: 1-5; the delicate flavor threshold value of the delicate flavor peptide is 0.2 to 0.5 mmol / L. According to the method, high-value utilization of the penaeus vannamei head byproducts is achieved, and the problems of wasting of aquatic product processing resources and environmental protection pain are effectively solved; five brand-new umami peptide sequences are obtained for the first time, the umami threshold value is far lower than that of monosodium glutamate, the synergistic fresh-increasing effect is outstanding, and the blank of an aquatic product source umami peptide library is filled.

The invention discloses umami peptide RCNG from litopenaeus vannamei. The amino acid sequence of the umami peptide RCNG is as shown in SEQ ID NO. 2. The umami peptide RCNG from the litopenaeus vannamei is applied to serving as a flavor enhancer or preparing the flavor enhancer. According to the present invention, the new umami peptide is explored from the litopenaeus vannamei meat protein sequence through the virtual screening, the molecular simulation and other technologies, such that the marine umami peptide library is enriched, the key amino acid combined with the umami peptide receptor and the action mechanism are researched, and the reference is provided for the research of the umami presentation mechanism of the peptides with different structures.

The application belongs to the field of aquatic animal germplasm protection and molecular breeding, and particularly relates to a kind of molecular marker related to Litopenaeus vannamei red shell color and application. The molecular marker is located on a chromosome, i.e. one or several of LvRE1, LvRE2 and LvRE3; the base sequence of LvRE1 is shown in SEQ ID No. 1, the base T at the 192th position in the sequence is mutated to C; the base sequence of LvRE2 is shown in SEQ ID No. 2, the base A at the 56th position in the sequence is mutated to G; the base sequence of LvRE3 is shown in SEQ ID No. 3, the base C at the 185th position in the sequence is mutated to T. The molecular marker related to Litopenaeus vannamei red shell color provided by the application has universality in germplasm from different sources, is simple to operate, has high accuracy, and has a broad application prospect in the genetic breeding research of Litopenaeus vannamei.

This invention relates to the field of aquaculture technology, and in particular to a biological attractant based on Schizochytrium fermentation broth and its preparation method; the biological attractant based on Schizochytrium fermentation broth is obtained by processing the fermentation broth after oil extraction from Schizochytrium; the fermentation broth after oil extraction from Schizochytrium is the residual fermentation broth after supercritical CO2 oil extraction or enzymatic hydrolysis oil extraction from Schizochytrium; the preparation method includes: pretreatment of fermentation broth, sterilization and stabilization treatment, concentration and regulation, and filling and storage; the three core components in the fermentation broth work synergistically, umami amino acids quickly trigger the taste perception of aquatic animals, algal polysaccharides slowly release odor molecules to prolong the feeding attraction time, and DHA enhances natural tropism, significantly improving the feeding induction rate of Litopenaeus vannamei, and maintaining a stable feeding attraction effect even in rainy and low-temperature environments; relying on the natural viscosity and structural characteristics of algal polysaccharides, there is no need to add stabilizers such as xanthan gum.

This invention provides a method for treating wastewater from Litopenaeus vannamei farming, comprising the following steps: S1, primary filtration of the farming wastewater, followed by aeration with the addition of compound bacteria to the filtered wastewater, controlling the aeration temperature at 30-35℃, dissolved oxygen at 5-10 mg / L, and treatment time at 10-16 h to obtain aerated wastewater; S2, mixing the aerated wastewater with composite activated carbon and treating it at 30-40℃ and ultrasonic power at 300-500 W for 2-3 h, then filtering to obtain filtrate A; S3, circulating filtrate A through a mixed macroporous adsorption resin at a loading flow rate of (3-5) BV / h to obtain filtrate B, followed by sterilization, thus completing the treatment of Litopenaeus vannamei farming wastewater. This invention effectively purifies Litopenaeus vannamei farming wastewater by adding compound bacteria for aeration treatment, combined with activated carbon and macroporous adsorption resin. This effectively reduces the content of pollutants such as total nitrogen, total phosphorus, and suspended solids in the wastewater, resulting in good treatment effect.