109 results about "Homozygous genotype" patented technology

The invention discloses a breeding method for inducing parthenogenesis of corn by trifluralin. The method is characterized by comprising the following steps of: selecting 48 percent trifluralin missible oil as an inducer, and diluting the trifluralin missible oil into 200-1,000-fold solution by using water; inducing, namely spraying or coating trifluralin diluent on thrums after the thrums on clusters come out; harvesting to obtain diploid seeds with homozygous genotype when seeds are mature; seeding in the next season, identifying truth according to phenotype and weeding out inferior plants,namely seeding the harvested clusters for the next generation, selectively harvesting rows according with a breeding goal, and thus obtaining a homozygous self-bred line of the corn. The invention has the advantages that: the trifluralin has low toxicity, can be used in the field under open conditions, and is safe to people, livestock and the environment; the inductivity is about 1.0 to 3.0 percent, pollination is not required, and the method is simple, easy to operate, low in cost and high in efficiency and can be used in a large scale; the diploid seeds are directly formed, and the process of doubling chromosomes is not required; and moreover, compared with that of the conventional seed selection method and a Stock 6 genetic induction method, the seed selection period is shortened by more than six generations and is shortened by more than one generation.

The invention relates to a breeding method for introducing corn parthenogernesis by using amiprofos-methyl, and belongs to the field of planting breeding. The method comprises the following steps of: preparing an inductive agent of which the concentration is 5-200mumol/L from the amiprofos-methyl and water; performing introducing treatment: when filaments sprout to grow to about 2.5mm, spraying or daubing the inductive agent to the filaments, and harvesting to obtain homozygous genotype diploid seeds when seeds are mature; and during seeding in the next season, selecting and identifying by using the conventional method to select head progeny rows according with the breeding aim to obtain a homozygous corn selfing line. The breeding method for introducing the corn parthenogernesis by using the amiprofos-methyl has the positive effects of avoiding the artificial pollination, and being simple in method, easy to operate, low in cost, high in efficiency, and capable of being applied in large scale; in addition, the diploid seeds are directly formed and a chromosome doubling process is not required; and in the breeding method, more than 5 generations are shortened in comparison with a breeding method of the conventional corn selfing line.

The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.

Methods for the use of Fc gamma receptor (FcγR) polymorphisms as a diagnostic for intervention with interleukin-2 (IL-2) immunotherapy are provided. The methods comprise detecting the allelic pattern of an FcγRIIIA gene or FcγRIIA gene of an individual, and determining whether the allelic pattern is predictive of a positive therapeutic response to IL-2 immunotherapy. The presence of the FcγRIIIA 158F/F homozygous genotype, and/or the presence of one or both copies of the FcγRIIIA 48L allele, and/or the presence of one or both copies of the FcγRIIA 131R allele is predictive of a positive therapeutic response to IL-2 immunotherapy, and therefore indicative of medical intervention with IL-2 immunotherapy for treatment of an immune disorder. The diagnostic method finds use in identifying those individuals whose immune function can be improved by treatment with IL-2 immunotherapy, particularly for individuals with cancer.

The invention discloses a breeding method for a high-yield new variety line of recessive white-feather gooses and application thereof. The method comprises the following steps of: selecting the high-yield white-feather gooses without broodiness or with weak broodiness, and eliminating dominant white-feather individuals and keeping recessive white-feather individuals A by the test cross or the molecular marker method; self-reproducing the male individuals and female individuals of A to breed the recessive white-feather gooses B; and breeding B again, eliminating dominant white-feather homozygote and heterozygote, self-reproducing the recessive white-feather high-yield individuals with homozygous genotype to expand the group, building a family and breeding the family until the high-yield recessive white-feather goose new variety line C is bred, and performing dualistic or polyphyletic mating by taking the C as a female parent and a colour-feather local goose as a male parent to produce colour-feather gooses or simulated local gooses.

The invention discloses a transgenic method using wheat anthers as receptors. The method provided by the invention comprises the following steps: 1) using plant anthers as receptors to carry out gene transformation with a gene gun method to obtain transformed anthers; 2) culturing the transformed anthers obtained in step 1) to obtain embryoid bodies, The embryoid bodies are then cultured to obtain haploid regenerated shoots. The present invention establishes a wheat transgenic technology system with anthers as receptors, in vitro moisturizing of anthers, adhesion and fixation of anthers during gene gun bombardment, gold powder particle size for preparing bullets, bullet coating effect, bombardment distance and haploid Chromosome doubling and other processes have been systematically studied, and transgenic plants with homozygous genotypes can be obtained in contemporary times.

The invention relates to an animal cultivation method, in particular to a cultivation method for a green-shelled-egg chicken pure line. The method adopts Jiuyuan black chickens as materials, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity. The method comprises the following steps: 1) selection of parents; 2) production of homozygosis green shell gene individuals; 3) expanding propagation of colony. The method has the advantages that the construction of green-shelled-egg chicken homozygosis colony is realized, homozygosis genotype green-shelled-egg chickens can be bred by screening heterozygosis genotype at the natural conditions and adopting a scientific breeding scheme, quick expanding propagation can be realized, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity.

The invention relates to bioinformatics and provides a disease susceptibility risk prediction method and device. The disease susceptibility risk prediction method comprises the steps: providing a database including disease morbidity data, SNP locus genotype frequency data, and risk allele homozygous and heterozygous genotype OR value data aiming at each disease-associated SNP locus; receiving information of a to-be-predicted individual; calculating to obtain a comprehensive disease susceptibility risk array of diseases interested by the to-be-predicted individual; and generating a comprehensive disease susceptibility risk dynamic change curve of the individual in an appointed age range. According to the disease susceptibility risk prediction method and device, two factors , including individual inheritance and environment factors, are considered at the same time when the disease susceptibility risk of an individual is calculated, so that calculating results greatly conform to objective reality; according to the obtained disease susceptibility risk age change curve of the individual, the individual not only can learn more precise immediate disease susceptibility risk, but also can continuously understand the susceptibility change tendency of various diseases along with the growth of age.

The present invention discloses a method for breeding the commercial strains of red feather pink-shell laying hens. It provides a primer pair for identifying the red feather causative mutation homozygous genotype of white leghorn chickens, which is composed of the single-stranded DNA molecule shown in Sequence 2 of the Sequence List and the single-stranded DNA molecule shown in Sequence 3 of the list. After the primer was designed according to the upstream and downstream nucleotide sequences of the 18,288,303^rd deoxynucleotide in the positive-sense strand of the 11^th chromosome as shown in the sequence information of the chicken reference genome Gallus_gallus-4.0 version published in NCBI, the genotype (SNP) at this site is tested through the restriction fragment length polymorphism, the genotype of the site (SNP) was tested through the restriction fragment length polymorphism; the offspring hens obtained by cross breeding the homogenous female parent (the homogenous female parent was obtained through expanded propagation of the white leghorn chickens with the red feather causative mutation homozygous genotype) and the Rhode Island Red rooster as a male parent are all of red feather phenotype, meeting the market demands and enjoying a broad prospect for promotion.

The invention provides a breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content, comprising the following steps: taking exarate pupae breeds (with Nd-s genes) as stuff to be hybridized with the breeds with high amount of silk production, and breeding Japanese special original strains with sericin content being more than 99.0% and homozygous genotypes (Nd-s/Nd-s) by adopting the methods of selfing, backcrossing, marker gene selecting, sericin content tracking and detecting and the like; and hybridizing the Japanese special original strains with the Chinese conventional breeds (Gc/Gc) with green cocoon properties to breed the dedicated silkworm hybrid. The method can provide high quality raw materials to such fields as cosmetics, medicines, food, fiber modified materials and the like and improve the product added value.

The invention belongs to the technical field of animal husbandry or animal genetic breeding and discloses identification and application methods for an MC1R gene haplotype. The identification method for the MC1R gene haplotype comprises the steps that an MC1R gene is subjected to PCR amplification, and single-ended Sanger sequencing is adopted for obtaining a variation sequence; based on the established haplotype, the genotype and phenotype of the hair color of a breeding group are subjected to association analysis, and the haplotype for controlling a black feather and the black feather homozygous genotype are identified. According to the identification method, the haplotype for controlling the black feather and the black feather homozygous genotype can be accurately identified. According to the identification method, key SNPs loci are simultaneously identified in one sequencing reaction; but in the prior art, two sequencing primers are used for simultaneously identifying the SNPs loci. Compared with a PCR-RFLP identification method in the prior art, through Sanger sequencing, the identification accuracy is further improved.

The invention provides a method for detecting genotype of chicken beard and relates to the field of molecular biology. The method provided by the invention is based on a pyrosequencing technology, a pair of primers and a sequencing primer with the amino acid sequences as shown in SEQ ID No. 1, 2 and 3 are adopted to analyze single nucleotide polymorphism of a 1707859bp locus of a chicken No. 27 chromosome, and the results find that the genotype of the chicken beard is that, dominant homozygotes have T bases and C bases, which have the same peak height and account for one half respectively; in heterozygotes, the peak height of T bases is greater than that of the C bases, and T: C=2: 1; and recessive homozygotes only have peaks of the T bases and no peaks of the C bases. The method provided by the invention is simple to operate, high in sensitivity, strong in accuracy, high in throughput and low in detection cost, can fast detect beard individuals with the homozygous genotype and has importable application value in chicken conservation and breeding processes.

The invention discloses a simplified seed production method of small-grain hybrid rice and a small-grain hybrid seed. The simplified seed production method comprises the following steps: 1) cultivating strong seedlings by using Bing 4114 as a male parent; 2) directly sowing the small-grain sterile line Zhuo 201S serving as a female parent in a field, and throwing the male parent subjected to seedling strengthening in the step 1) into the field with the female parent; and 3) after maturity, harvesting the male parent and the female parent in a mixed manner, and screening and separating to obtain the small-grain hybrids. According to the simplified seed production method of the small-grain hybrid rice, disclosed by the invention, the Bing 4114 with high combining ability, strong disease resistance and homozygous genotype is selected as the male parent, and the small-grain sterile line Zhuo 201S with high stigma exsertion rate, good outcrossing characteristic and excellent agronomic traits is selected as the female parent for mixed sowing hybrid seed production; the high-yield, high-quality and disease-resistant rice small-grain hybrid seeds are obtained, male parents and female parents of the small-grain hybrid seeds are subjected to mixed sowing and mixed harvesting, male parent and female parent seed separation is achieved in combination with screening and separation, and therefore simplified seed production of the small-grain hybrid seeds is achieved.

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker closely related to the rape flower color traits and application of the molecular marker. The molecular marker comprises a forward primer and a reverse primer. With total DNA as a template, PCR amplification is performed on the molecular marker and electrophoretic separation is performed; a single plant only having a single stripe of 180 bp is a homozygous yellow-flower individual, a single plant only having a single stripe of 177 bp is a homozygous white-flower individual, anda single plant having the two stripes at the same time is a heterozygous individual. The molecular marker developed by the invention can be applied to identification and prediction of a white-flower variety and a yellow-flower variety of rape; the operation is simple and convenient; a method is easy to implement; the problem of identification between a heterozygous genotype and a homozygous genotype can be effectively solved; offspring selection is more pertinent; the selection efficiency is improved.

The invention discloses a wheat molecular marker and application thereof in identifying wheat yield related traits. According to the wheat molecular marker, a primer pair P is utilized to perform amplification by using wheat genome DNA (deoxyribonucleic acid) as a template to obtain DNA molecules. The primer pair P is composed of single-stranded DNAs p1 and p2, wherein the p1 is a single-stranded DNA which is specifically combined with the forward 70th site disclosed as Sequence 1 in the wheat genome DNA, and the p2 is a single-stranded DNA which is specifically combined with the reverse 241st site disclosed as Sequence 1 in the wheat genome DNA. The wheat is subjected to genotyping according to the wheat molecular marker to obtain the three homozygous genotypes A1A1, A2A2 and A3A3. The experiment proves that the genotypes A1A1, A2A2 and A3A3 of the wheat are related to wheat yield related traits-number of spikelets per spike, number of ears per plant and weight of thousand grains, and can be used for culturing high-yield wheat species.

The invention discloses a method for detecting Marek's disease resistance homozygous genotype chicken, which comprises the following steps: adopting the PCR-RFLP technology to perform PCR amplification on the second exon of BLB2 genes of 99 MDV-1 toxin eliminated Xiayan chickens and perform Alu I, Cai I, Cfr I, Hinl I, Hinf I and Rsa I restriction enzyme analysis on the PCR products, and initially obtaining seven BLB2 allelic genes genotype chickens by performing the restriction analysis on the six loci; finally obtaining six BLB2/BF2 allelic genes genotype chickens by sequencing the BLB2 and BF2 genes of the seven genotype chickens; and by combining the toxin-eliminating result, determining four MD resistance individuals. The established method can detect the MD resistance homozygous genotype chickens, and the MD resistance homozygous genotype chickens are taken as breeders to breed the MD resistance chickens. Therefore, the method has extremely great economic benefits to prevention and control of MD and related immune-suppressing diseases on genetic predisposition.

The invention discloses a breeding method for selecting high-production-performance A2A2 homozygous genotype dairy cattle based on pedigree information simplification. By performing dairy cattle production performance measurement, pedigree data and phenotype data of dairy cattle individuals are collected, on the basis of a father beta casein genotype result, genetic evaluation is performed, breeding values of the milk yield, the butterfat yield, the milk protein yield and the milk protein rate are obtained, and high-yield A2A2 homozygous individuals are obtained through analysis by calculatingan individual high production index HPI. According to the method, by applying daily DHI data for large group screening, the workloads of the actual cattle farm production process are greatly lowered;the A2A2 homozygous individuals in the cattle herd can be rapidly, conveniently and accurately screened out.

The invention discloses co-dominant SSR (simple sequence repeat) markers closely interlinked with a major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum and application ofthe co-dominant SSR markers. The serial numbers of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum are TM22038, TM23004 and TM22041, and nucleotide sequences of PCR (polymerase chain reaction) amplification products of the markers are respectively shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 and SEQ IDNo.4, SEQ ID No.5 and SEQ ID No.6. The application refers to application of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum in detecting whether nicotiana tabacum genome DNA (deoxyribonucleic acid) has a homozygous genotype nic1 or not and a nicotiana tabacum variety of a low nicotine content is available or not. The co-dominant SSR markers disclosed by the invention have the characteristics of being stable, reliable, simple and convenient, rapid and low in cost, so that the molecular markers can be applied to assistant selection of molecular markers of the low nicotine content gene nic1 in high-quality nicotiana tabacum breeding.

The invention relates to a four-primer molecular marker method for identifying ALS (acetolactate synthase) gene mutation generation of rice, and belongs to the field of biotechnology engineering. According to the variation of the rice ALS genes at 1642nd and 1643rd basic groups in a coding region from TG to AT, four specific molecular marker primers are designed and are added into a same PCR (polymerase chain reaction) system; different rice DNA (deoxyribonucleic acid) is subjected to amplification; electrophoresis detection is performed; DNA stripes with different sizes represent homozygotes and heterozygotes with different genotypes of the ALS. The method can be used for fast and accurately identifying the anti-ALS inhibitor type herbicide type gene variation occurring in the rice germplasm resources or breeding groups; in addition, the selection efficiency on the ALS-gene-mutation-containing homozygous genotypes can be improved in the breeding process; the breeding process of the anti-ALS inhibitor type herbicide rice varieties is accelerated.

The invention relates to the technical field of molecular identification of fish, in particular to an SNP marker associated with vibrio harveyi disease of large yellow croaker and primers and applications thereof, wherein the nucleotide sequence of the SNP marker is shown as SEQ ID NO. 1, and the nucleotide sequence starts from base G or T at position 343 from 5 'end. The invention overcomes the problem that there is no SNP marker which can be used for the selection and breeding of disease resistance related traits of large yellow croaker in the prior art, and at the SNP site of the invention,the probability of the large yellow croaker with the genotype of GT to be infected with Vibrio harveyi is significantly higher than that of the homozygous GG genotype individual. Therefore, by detecting the SNP of Pseudosciaena crocea, it can be effectively determined whether it is susceptible to Vibrio harveyi infection. Elimination of GT genotype individual in parent breeding is favorable for improve that ability of offspring to resist infection of Vibrio harveyi, and the marker of the invention is used for auxiliary breed, thereby accelerating the cultivation process of disease-resistant varieties of large yellow croaker.

The invention discloses a molecular marker related to growth and development characters of broilers and application thereof. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:1, and the mutation of the molecular marker is located at exon 1c.409G>A (p.Ala137Thr). The invention discovers that the growth of the broilers is further blocked due to the mitochondrial DNA depletion caused by the TWNK gene mutation, and the production character of the broilers is influenced. Therefore, the molecular marker can be applied to eliminating stiff chickens, and the application comprises the following steps of: S1, extracting DNA of a chicken to be detected; S2, acquiring and sequencing a target fragment: amplifying DNA of the chicken to be detected through PCR by using a forward and reverse primer pair, sequencing the obtained product, and analyzing sequence bases; and S3, according to the analysis result, selecting individuals which are determined to retain the GG genotype during breeding. The molecular marker is favorable for breeding dominant homozygous genotype broilers and improveing the production performance of the broilers.

The invention relates to the technical field of biology, and in particular to a method for cultivating a japonica rice variety resistant to herbicide imazethapyr assisted by molecular marker. The method comprises the steps of screening an anti-imazethapyr rice material Jinjing 818 from 7403 parts of rice varieties resource materials, taking Jinjing 818 of japonica rice variety resistant to herbicide imazethapyr as a donor and conventional japonica rice variety as an acceptor for crossbreeding, obtaining F2 generation group through F1 hybrid selfing, using SSR molecular marker RM7413 to choose an individual corresponding to a linked molecular marker band of the donor Jinjing 818 herbicide resistance gene, namely homozygous genotype F2 generation strain and obtaining the stable japonica rice new variety resistant to the herbicide imazethapyr through F2 generation strain continuous selfing until obtain high-generation groups. The SSR molecular marker RM7413 and the molecular marker assisted cultivation method obtained by the invention will play an important role in genetic improvement of the japonica rice resistant to the herbicide imazethapyr.

The invention relates to a PCR/LDR molecular marker and method for identifying a high-temperature-resistant TT1 genotype of rice, and belongs to the technical field of rice breeding. The molecular marker comprises a pair of PCR primers and three LDR probes, the forward primer sequence of the PCR primer pair is shown as SEQ ID NO. 1, and the reverse primer sequence is shown as SEQ ID NO. 2. The LDRprobes comprise a fluorescence labeling probe Probe-FAM of which the sequence is shown as SEQ ID NO.3, a non-high-temperature-resistant variety allele specific probe Probe-OsTT1 of which the sequenceis shown as SEQ ID NO.4, and a high-temperature-resistant allele specific probe Probe-OgTT1 of which the sequence is shown as SEQ ID NO.5. The method disclosed by the invention not only can accurately and quickly identify rice varieties and the genotype of the high-temperature-resistant gene TT1 in each strain in a breeding population, but also can realize high-throughput detection of more samples. Compared with a conventional molecular marker group, the molecular marker has the advantages of simplicity, convenience, quickness and high efficiency. The method provided by the invention can improve the selection efficiency of the high-temperature-resistant OgTT1 homozygous genotype rice strain, and accelerates the breeding process of the high-temperature-resistant rice variety.

The invention discloses a new soft rice waxy gene wx-C39 functional marker and application thereof, and belongs to the field of agricultural biological engineering. A molecular marker primer is WxC39-F:CGTCCCAGCTCGCCACCT and WxC39-R:CCACGCTGCACCGACCG, and if an amplification product is 173bp, it is indicated that wx-C39 homozygous genotype is carried, wherein the amylose content is about 5%; if 184bp is obtained through amplification, it is indicated that a homozygous genotype of Waxy is carried, wherein the amylose content is about 15%; if two bands are amplified simultaneously, it is indicated that a heterozygous genotype exists, wherein the amylose content is about 15%. The marker and a method can be used for filial generation genotype detection, and the soft rice breeding process can be accelerated conveniently. The method is high in accuracy, easy to operate, high in detection efficiency and low in cost, and has very high practical value.