379 results about "Sericin" patented technology

There is provided a novel wound dressing material which has biocompatibility and infection controllability as essential properties required for such a material, especially excellent flexibility and water absorption properties, thereby accelerating smooth regeneration of a skin defect without stripping off the regenerating skin while removing the material from the skin. A healing agent is added to the wound dressing material which comprises an amorphous film of a crystallinity below 10% and contains fibroin and sericin as a main component.

The present invention relates to a membrane for guided bone tissue regeneration and, more particularly, to a membrane for guided bone tissue regeneration having a structure that silk fibroin nanofibers obtained by removing sericin from silk fibers are formed as a nonwoven, and a manufacturing method thereof. A membrane for guided bone tissue regeneration according to the present invention has a predetermined strength, biocompatibility, and biodegradability, and may maintain a sustained drug release system, when drugs are added in the manufacturing process. Additionally, a membrane for guided bone tissue regeneration according to the present invention may be modified corresponding to the condition of usage, because a thickness of the membrane may be adjusted by controlling fineness of nanofibers, compactness of nanofibers, and pore size of a multiporous structure may be adjusted, in a nonwoven manufacturing process. A nanofibrous membrane for guided bone tissue regeneration according to the present invention is manufactured by freezing rapidly, drying a silk fibroin solution obtained by removing sericin from silk fibers, and by electrospinning after dissolving the dried silk fibroin in an electrospinning solvent. The membrane according to the present invention has excellent adhesion and air permeability, and is thereby effective in regeneration of damaged periodontal tissues.

A process for producing non-woven silk fiber fabrics comprises the following steps: a) obtaining silk fibroin, for example either from silk cocoons, or silk textiles or waste silk; b) removing the sericin layer covering the silk fibroin fibers, when present; c) breaking the disulfide bonds between heavy (350 kDa) and light (27 kDa) chains of silk fibroin in order to obtain the production of chain fragments which serve as a specific cellular recognition sites promoting the attachment and growth of cells, d) homogenising of the material resulting from step c).

The invention relates to a silk producing method which comprises the steps of: high-temperature direct steaming: processing silkworm cocoons with saturated steam in an direct steaming chamber or a direct steaming device, wherein the direct steaming temperature is 100 DEG C, the direct steaming time is 8 to 10 minutes; standing: standing the directly-steamed silkworm cocoons at a normal temperature for 18 to 26 hours; vacuum infiltration: infiltrating the stood silkworm cocoons by utilizing a vacuum infiltration method to subject the silkworm cocoons to absorbing water, wherein the infiltration water temperature is 35 DEG C to 40 DEG C, the infiltration time is 5 to 10 minutes, and the vacuum degree is 0.05 to 0.1 Mpa (megapascal); cocoon cooking: cooking the silkworm cocoons subjected to vacuum infiltration in a cocoon cooking device; silk reeling: dissociating cocoon filaments of the cooked silkworm cocoons at a temperature of 40 DEG C, and utilizing sericin to glue and connect the cocoon filaments to form raw silk; and post-reeling which includes steps of roughness removal, small reel silk sheet balancing, small reel vacuum moisture absorption, rereeling, large reel silk sheet balancing, arranging and the like. The silk producing method enables the neatness of the raw silk to be relatively high by utilizing high-temperature direct steaming and vacuum infiltration.

The invention discloses a natural rubber liquid slurry containing chitosan/sericin and a preparation method thereof, and belongs to the field of high polymer chemistry. The invention is characterized in that the preparation method comprises the following steps: respectively preparing a chitosan water solution and a sericin water solution, mixing the two solution, and regulating the pH value of the mixture to 9-11 by using ammonia water and a citric acid solution; and adding the obtained chitosan/sericin mixed solution to a natural latex slurry, adding a laurate surfactant, and mechanically stirring the mixture uniformly to obtain the natural rubber liquid slurry containing chitosan/sericin. The invention has the advantages that the chitosan has the functions of adsorbing water soluble proteins in the latex, inhibiting bacteria and viruses from breeding, and the like; and the sericin has the functions of oxidation resistance, allergy resistance, hydrophilia, oil resistance and the like, thereby enhancing the emulsion phase stability and the preservation effect of the natural latex. In particular, the product obtained by using the method overcomes the defects of short service life, easy aging and sticking, poor oil resistance, initiation of protein allergy and the like in the traditional natural latex product.

The invention relates to a method for producing mulberry silk quilt which can be washed via water. Wherein, it comprises that degelatinizing the pod and using cotton machie to prepare cotton sheets, arranging cotton sheet in normal temperature to expand sericin; degelatinizing the cottom sheet; cooling, poaching, and neutralizing, adding flexible agent, filtering; adding water-resistant agent, drying at 100-150Deg. C for 30-60mins; tensioning the dried cotton sheet, to expand the cotton sheet, and packing it into coat.

The invention relates to silk property-variable non-woven cotton with sericin and relative application. Said silk property-variable non-woven cotton via selection-modifying-blanching, and washing-carding-needling processes can be made into the silk property-variable non-woven cotton which will not lose silk. Said non-woven cotton can recover water-solubility and biological activity via protein recover treatment to supply the health function of silk protein, and apply the medical, hairdressing and daily consumption areas. Since said invention can keep a lot of sericin protein, therefore, it has lower cost, better fiber flexibility and thermal insulation than traditional silk non-woven cotton, and it can avoid de-gumming process to reduce environment pollution with better economic, social and zoology benefits

The invention discloses a cleaning cocoon cooking method. The method comprises the processes of: external vacuum infiltration, cooking by an automatic cooker, cocoon steaming, and adjustment, wherein a reelability agent originally used for cocoon cooking replaces a penetrating agent for penetrating to be used in the external vacuum infiltration cocoon cooking process; in the condition with 30-50 DEG C water temperature, the reelability agent can only have infiltration function with water to be adsorbed between cocoon layers and on the surface of a cocoon filament; and in the cocoon cooking process and in the condition with 60-80 DEG C water temperature, the reelability agent can promote the cocoon filament sericin dissolution to reduce adhesion between cocoon filaments for the smooth dissociation of the cocoon filaments, so that the reelability agent has the reelability function in the cocoon cooking process. One chemical preparation achieves two functions, the chemical preparation is less and easily controlled in dose, process is simple, operation is convenient, and production cost is low.

Provided is an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.

A gel film of polyvinyl alcohol and sericin mixture with uniform microstructure, high mechanical performance and high bioactivity is prepared through respectively preparing polyvinyl alcohol solution and sericin solution, proportional mixing, stirring, pouring in a container with flat bottom, sealing, and freezing-thawing several times.

The invention relates to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and a preparation method thereof. The hydro-gel comprises a sericin polypeptide chain part, an inorganic nano-soapstone particle part and a polymer formed after an alkylacrylamide monomer is polymerized. In the hydro-gel, a semi-interpenetrating network technology is mainly adopted, and by combining the physical crosslinking effect of inorganic nano-soapstone particles, the sericin, the inorganic nano-soapstone particles and in-situ free radical of the alkylacrylamide monomer are polymerized. The preparation method comprises the following steps of: dispersing the inorganic nano-soapstone particles; preparing a reaction prepolymerization solution; preparing gel; and purifying the gel. The sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel has rich raw material sources, simple preparation method, low cost, high swelling degree and excellent cell compatibility, can be adjusted according to the content of sericin, and has a wide application prospect in artificial skin, artificial cartilage, cell culture stent, bioreactor and other biomedical aspects.

The invention discloses a tussah cocoon shell bio-enzyme degumming method which is composed of the steps that: clear water is added into a degumming tank body; the clear water in the degumming tank body is heated to 45-50 DEG C; soda ash is added into the degumming tank body, and the mixture is well mixed, such that the pH value of the water in a cocoon boiling tank reaches 9.0; 2709 alkaline protease is added into the degumming tank body, such that a degumming liquid is obtained; cocoon shells are added into the degumming tank body, and are completely immersed in the degumming liquid; the mixture is continuously stirred, and degumming temperature is maintained at 45-50 DEG C; soaking is carried out for 3-4h; when silk residual gum is lower than or equal to 3%, the silk is fetched, and degumming is finished. When cocoon shells are subjected to a degumming process by using the tussah cocoon shell bio-enzyme degumming method, production cost is low, degummed silk quality is good, and sericin in the degumming liquid can be recovered.

The invention relates to the technical field of photo-curing hydrogels, and in particular relates to a visible light initiating system in a medical photo-curing hydrogel and a photo-curing method thereof. The visible light initiating system in the medical photo-curing hydrogel contains the following components in part by weight: 0.5-1 part of the first component: photosensitizer, and 0.5-5 parts of the second component: auxiliary initiator; the auxiliary initiator is water-soluble sericin or fibroin powder, the photosensitive synergist is one of iodonium salt, sulfoniumsalt or phosonium salt. The system is good in solubleness in water, and excellent in biocompatibility or biosecurity; and the free radical produced after absorbing 400-700nm of visible light can efficiently initiate the polymerization and curing of the precursor aqueous solution containing unsaturated double bond, thereby preparing the multipurpose medical hydrogel.

The present invention discloses preparation process of skin care silk protein gel cream. The preparation process includes the following steps: adding water soluble sericin or water soluble sericin and silk peptide as gel promoter into water solution of fimbrin through homogeneous mixing; stilling the solution at 0-90deg.c for gelatinizing the solution into translucent silk protein gel with water content of 70-98 %; and homogenizing and emulsifying to form silk protein gel cream. Into the silk protein gel cream, other cosmetics matrix component and supplementary material component may be added as required. The silk protein gel cream has the functions as nutrient additive, humectant and matrix, and may be used in producing high quality natural skin care cosmetics.

A process for removing sericin from silkworm cocoons includes such steps as adding silkworm cocoons into a rotary container, sealing the container, high-temp. and high-pressure treating for a certain, time to strip sericin from silk, suddenly releasing pressure to make the cocoon become puffed silk floss separated from silk and sericin, separating sericin from silk, and dewatering each of them. Its apparatus is composed of the container with exploding membrane on the cover and drier.

The invention relates to a process for producing a silk quilt by utilizing sericin, which comprises the following steps of: sequentially carrying out vacuum penetration and cocoon cooking on raw material cocoons; then carrying out one-particle hemming to form a yarn sheet; carrying out sericin fixation processing on the yarn sheet to enable the sericin to be fixed; and after carrying out post-treatment, drawing to form a silk quilt inner tube. The process is characterized in that in both the vacuum penetration and cocoon cooking processes, a raw silk softener is added; the yarn sheet is timely dried by infrared light or hot air after one-particle hemming is carried out to form the yarn sheet; before the sericin is fixed, the yarn sheet has sericin content of 15 percent to 26 percent; and the sericin is fixed by a cross-linking agent. According to the invention, the sericin can be reserved to the greatest degree; and denatured proteins of the silk quilt can be reduced, and silk fibers containing the sericin not only are soft, but also have stable performance.

The invention belongs to the technical field of biological medicine materials, and discloses a tumor targeted sericin micelle, and a preparation method and an application thereof. According to the method provided by the invention, with water soluble natural protein sericin having good biocompatibility and biodegradability as a raw material, an amphiphilic sericin derivative containing a hydrophobic cholesterol group is synthesized under a room temperature condition, folic acid is further coupled by a chemical reaction to prepare a targeted amphiphilic sericin derivative, and a drug-loaded nanometer micelle can be formed with a hydrophobic drug IR780 in an aqueous solution by means of the self assembly. The nanometer micelle system can effectively improve the stability of the drug in the blood circulation and reduce the toxic and side effects of the drug, thereby having a very good research value and application prospect in the preparation of tumor diagnostic agents or treatment drugs.

This invention discloses one kind of silk textile fiber and its fabric fining come unglued method. Front it uses the low temperature plasma technology carries on to the raw silk textile fiber and its the fabric processes, substitutes the conventional fining come unglued center's pretreatment, initially practices working procedure, then the direct process duplicate practices, achieves to the silk textile fiber and its the fabric concise come unglued's goal. Because the low temperature plasma physical ability sericin the macro-molecule carries on to the raw silk textile fiber and its fabric surface shells and splatters sculpture, causes its stopped chain or sculpture dehiscence, loose, duplicate causes to practice the fining medicinal preparation to be able fast to seep, bulked, degrades (hydrolisis or enzymolysis) sericin, therefore, has the craft simply, the energy conservation is highly effective, is advantageous to the clean production and the environmental protection merit.

The method for preparing water soluble sericin with different molecular weights by using cocoon as raw material includes the following steps: soaking cleaned cocoon crust and waste silk in water or alkalescency solution, regulating pH value of said solution to 8-12 and making high-temp. high-pressure degrading treatment, regulating temp. or pressure, treatment time and pH value of boiling solution and controlling requirement molecular weight of water soluble sericin so as to prepare water soluble sericin solution or powder. The product can be extensively used in the fields of biological material, textile finishing agent.

The invention provides a method for selecting and breeding a novel silkworm breed capable of producing a great number of all natural pure sericin proteins, which introduces a special germ plasm resource from Japan, adopts genetic thremmatology to be combined with bioscience, and utilizes a distant hybridization method to breed a silkworm breed with sturdy, disease prevention, high temperature resistance, short breeding period and excellent economic character. The method leads excellent genes of the silkworm breed in the germ plasm resource to breed into an all natural pure sericin silkworm breed with high purity, and provides a novel approach for non spun silk industries to produce all natural pure siricin materials.