12 results about "Silk gland" patented technology

The invention belongs to the technical field of biology, particularly discloses a method for preparing spider silk protein-containing composite silk by using transgenic silkworms and application of the spider silk protein-containing composite silk, and particularly provides a method for synthesizing and secreting spider silk protein by using a silkworm silk gland bioreactor. The spider silk protein gene is introduced into a bombyx mori genome in a virus infection manner by utilizing a transgenic bombyx mori technology and is specifically expressed in bombyx mori silk gland cells, and the spider silk protein target gene can be introduced in batches by virtue of a lentivirus transfection method, so that bombyx mori capable of synthesizing and secreting complete spider silk protein can be developed on a large scale; the spider silk protein is synthesized and secreted through bombyx mori silk gland cells and enters silkworm cocoons along with the spinning and cocooning behaviors of bombyx mori, the silk is collected, the spider silk protein-containing composite silk with the bombyx mori silk as the background is obtained, a foundation is laid for mass production of black spider silk protein, and meanwhile a foundation is laid for improving the mechanical performance of the silk.

The application discloses a silk material with a cell proliferation function and application thereof, the functional material is expressed by a human type I collagen truncated fragment in a middle silk gland of a domestic silkworm, a sericin promoter is used as a tissue-specific promoter to drive specific expression of an exogenous gene in the middle silk gland, and finally the exogenous gene is combined with sericin protein and is secreted to outside of the body, the exogenous protein can be separated after a certain treatment of a cocoon, and the expression product can keep biological activity. The silk material is used to prepare a hydrogel, has a function of promoting wound healing, and can be applied to aspects of promoting skin regeneration and repair, bone graft substitutes, cartilage reconstruction, artificial skin and the like.

The invention discloses a viscous tussah silk gland protein gel dressing with microenvironment response and antibacterial property and a preparation method thereof, N-isopropylacrylamide is polymerized to form poly-N-isopropylacrylamide through in-situ free radical polymerization, and meanwhile, the poly-N-isopropylacrylamide, tussah silk gland protein and sodium alginate form gel in a stable form through intermolecular interaction; on one hand, the gel dressing has good temperature-sensitive response performance and realizes intelligent drug controlled release, and on the other hand, the hydrogel can be well adhered to the skin surface, can promote growth and reproduction of human skin fibroblasts, shows good biocompatibility and mechanical properties, has better adhesion stress than commercial wound dressings, and has good application prospects. An application basis is provided for the application of the sodium alginate group in the intelligent medicine controlled release medical wound dressing; finally, the gel dressing is loaded with a natural antibacterial agent and Chinese herbal medicines, and has the effects of resisting bacteria, diminishing inflammation and the like at the affected part; therefore, the gel dressing disclosed by the invention has great application potential in the medical field.

The invention discloses a construction method of bombyx mori for producing recombinant human III-type collagen and application of the bombyx mori, and belongs to the technical field of biology. On the basis of a bombyx mori silk gland bioreactor for efficiently synthesizing foreign proteins, specific expression of partial or complete full-length recombinant human III-type collagen in bombyx mori posterior silk glands is realized through a piggyBac mediated transgenic technology, and the recombinant human III-type collagen is successfully secreted into silkworm cocoons. The recombinant human III-type collagen which is single in component and high in safety is obtained through subsequent extraction and purification of posterior silk glands and silkworm cocoons, a novel biological manufacturing method of high-added-value protein is constructed, and meanwhile, the economic value of silkworms is greatly improved.

The application discloses application of a silkworm silk gland BmSPI45 protein in preparation of a product resisting red hair moss, and relates to the technical field of bioengineering, and has the technical scheme as follows: the application of the silkworm silk gland BmSPI45 protein in preparation of the product resisting red hair moss, and the amino acid sequence of the silkworm silk gland BmSPI45 protein is SEQ ID NO:1.The application uses an insect baculovirus expression system to perform in-vitro expression on a BmSPI45 recombinant protein, and obtains a recombinant pFastBac-BmSPI45 eukaryotic expression vector.The constructed pFastBac-BmSPI45 eukaryotic expression vector is transformed into a DH10Bac competent cell, and a recombinant Bacmid is extracted and obtained, the recombinant Bacmid is transfected, and the BmSPI45 protein is massively expressed.The BmSPI45 recombinant protein is subjected to antibacterial function research, and the result shows that the protein has the effect of resisting red hair moss, thereby providing a new protein alternative for development of an antibacterial preparation.

The application discloses application of specific knockout of a P53 gene in improvement of silk yield and a method thereof, and promotes development of a silkworm silk gland, increases expression of a silk protein gene and improves silk yield by specifically knocking out the P53 gene in the rear part of the silkworm silk gland through a CRISPR / Cas9 gene editing system, so as to provide a new breakthrough point and target for silkworm variety improvement aiming at improving a silk protein synthesis level and silk yield, and has a good application prospect.

The present application discloses silk improved by a spider silk protein gene and a method for using a spider silk protein gene to improve silk. The silk improved by the spider silk protein gene comprises a silk protein light chain. The silk protein light chain is linked to a spider silk protein which is formed by fusion expression with the silk fibroin light chain. An amino acid sequence of the spider silk protein comprises a repeat region sequence having at least one spider silk protein repeat unit sequence. The present application achieves the expression of Nephila pilipes pyriform silk in Bombyx mori silk fibroin, implements formation of an intact spider silk protein on the silk light chain, and expands sites for Bombyx mori silk gland gene editing. Light-chain gene edited Bombyx mori and a heavy-chain gene edited silkworm can be hybridized, thereby providing technical support for further selective breeding and optimization of fiber performance.

The invention discloses a method for producing an active rhBMP9 bone repair material based on a double-transgene bombyx mori silk gland biosynthesis system as well as a product and application of the active rhBMP9 bone repair material, and realizes cooperative expression of complex precursor protein Pro-mhBMP9 and precursor protein invertase hFurin in bombyx mori posterior silk glands. An hBMP9 / hFurin double-gene biosynthesis system capable of completing precursor protein shearing, maturation and functionalized silk material synthesis in vivo is established, the rhBMP9 / CaP / Sericin composite scaffold is further constructed by extracting silk protein of double-gene transformation bombyx mori, and the rhBMP9 / CaP / Sericin composite scaffold shows excellent bone repair and angiogenesis capacities in both the cell level and the animal level, and can be used for preparing a bone repair material. And a solid experimental and theoretical basis is provided for a functional protein-material-tissue regeneration integrated strategy based on a silk gland biosynthesis system.

This invention discloses a cell marker gene for maintaining the ion environment in silk-secreting insects. KCNQ Identification and application of marker genes for maintaining ion environment in silk-secreting insects KCNQ The gene is as shown in a) or b) below: a) the nucleotide sequence is as shown in SEQ ID No. 1; b) a protein encoding the amino acid sequence shown in SEQ ID No. 2, or a protein encoding a potassium channel activity obtained by substitution, deletion, and / or addition of one or more amino acid residues. This invention has discovered... KCNQ The present invention maintains cell-specific high expression in an ionic environment. KCNQ The gene is homologously conserved in various silk-secreting insects and is specifically expressed in the anterior silk gland. It can be used for the precise identification of anterior silk gland cell types in silk-secreting insects, the molecular improvement of silk fiber properties, and the development of multifunctional silk protein materials, which has important application value.

Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.

The invention discloses a spider silk protein transgene production method and application based on lentiviral transfection and sperm mediation in the field of biotechnology and genetic engineering. The spider silk protein transgene production method comprises the following steps: firstly, constructing a vector pLC-[3xP3-DsRed]-MaSp2-Amyloids for synthesizing and secreting spider silk protein genes by bombyx mori; the plasmid is introduced into a silkworm fertilized egg by using a lentivirus transfection and sperm-mediated gene transfer method, the red fluorescent protein gene and the black widow spider dragline silk protein 2 and amyloid polypeptide composite gene are introduced into a silkworm genome by using the characteristics of the lentivirus, and stable inheritance and expression are obtained; therefore, a transgenic silkworm capable of specifically synthesizing and secreting spider silk protein in bombyx mori silk gland cells is created, then the spider silk protein is synthesized and secreted by the silkworm, and the spider silk protein-containing composite silk with bombyx mori silk as the background is obtained. The method lays a foundation for mass production of black spider silk protein and also lays a foundation for improvement of mechanical properties of silk fibers.