3 results about "Silk gland" patented technology

The present application belongs to the field of silkworm genetic breeding and biotechnology, and particularly relates to a breeding method for breeding silk sericin cocoon producing silkworm varieties. The method first uses a piggyBac transposon mediated GAL4 / UAS transgenic system to create a homozygous promoter material carrying a silk gland specific promoter driven GAL4 and a homozygous functional gene material carrying a UAS regulated BmHR3 gene; then restores the economic traits of the materials through multiple generations of backcrossing, and obtains a homozygote through self-crossing and test-crossing; finally, the two homozygous materials are crossed to obtain a first generation hybrid. The silk sericin cocoon occurrence rate of the cocoon of the hybrid reaches 100%, the cocoon layer silk sericin content is greater than or equal to 99.5%, and the production traits such as healthiness and whole cocoon weight reach a practical level. The present application provides a complete, efficient and repeatable breeding scheme, and provides germplasm resources and technical support for the industrialized production of silk sericin cocoon.

The application discloses application of a silkworm silk gland BmSPI45 protein in preparation of a product resisting red hair moss, and relates to the technical field of bioengineering, and has the technical scheme as follows: the application of the silkworm silk gland BmSPI45 protein in preparation of the product resisting red hair moss, and the amino acid sequence of the silkworm silk gland BmSPI45 protein is SEQ ID NO:1.The application uses an insect baculovirus expression system to perform in-vitro expression on a BmSPI45 recombinant protein, and obtains a recombinant pFastBac-BmSPI45 eukaryotic expression vector.The constructed pFastBac-BmSPI45 eukaryotic expression vector is transformed into a DH10Bac competent cell, and a recombinant Bacmid is extracted and obtained, the recombinant Bacmid is transfected, and the BmSPI45 protein is massively expressed.The BmSPI45 recombinant protein is subjected to antibacterial function research, and the result shows that the protein has the effect of resisting red hair moss, thereby providing a new protein alternative for development of an antibacterial preparation.

This invention discloses a cell marker gene for maintaining the ion environment in silk-secreting insects. KCNQ Identification and application of marker genes for maintaining ion environment in silk-secreting insects KCNQ The gene is as shown in a) or b) below: a) the nucleotide sequence is as shown in SEQ ID No. 1; b) a protein encoding the amino acid sequence shown in SEQ ID No. 2, or a protein encoding a potassium channel activity obtained by substitution, deletion, and / or addition of one or more amino acid residues. This invention has discovered... KCNQ The present invention maintains cell-specific high expression in an ionic environment. KCNQ The gene is homologously conserved in various silk-secreting insects and is specifically expressed in the anterior silk gland. It can be used for the precise identification of anterior silk gland cell types in silk-secreting insects, the molecular improvement of silk fiber properties, and the development of multifunctional silk protein materials, which has important application value.