476 results about "Transcriptome Sequencing" patented technology

The invention belongs to the technical field of gene engineering, and relates to a cyperus esculentus CeMYB154 gene capable of improving salt tolerance and active oxygen scavenging capacity and application of the cyperus esculentus CeMYB154 gene. The Cyperus esculentus CeMYB154 gene belongs to an MYB transcription factor family, the Cyperus esculentus CeMYB154 gene is screened and transformed into Arabidopsis thaliana through transcriptome sequencing, bioinformatics analysis, gene cloning, overexpression vector construction and recombinant strain overexpression, and analysis finds that under salt stress, compared with a wild type, the root length of a plant with the overexpressed CeMYB154 gene is obviously increased, the growth vigor is better, and the growth rate of the plant with the overexpressed CeMYB154 gene is higher. According to the present invention, the content of MDA and H2O2 is significantly reduced, and the activity of CAT, POD and SOD antioxidant enzymes is significantly enhanced, such that the overexpressed CeMYB154 gene can enhance the salt tolerance and the active oxygen scavenging ability of the plant, and the overexpressed CeMYB154 gene is proved to be the salt tolerance positive regulation factor; the CeMYB154 gene can be used in the field of salt-tolerant stress-resistant breeding of cyperus esculentus, and provides an important gene resource for salt-tolerant molecular breeding.

The invention provides a clustering method and system for transcriptome sequencing data. The method is applied to the technical field of data processing, and comprises the following steps: collecting cervical adenocarcinoma and para-carcinoma tissue specimens of a plurality of patients, and carrying out data preprocessing to obtain a standardized gene expression matrix; performing clustering operation on the standardized gene expression matrix based on a clustering analysis method combining a potential category model and sub-alliance division to obtain a clustered gene set; performing gene function annotation on the clustered gene set, and performing correlation analysis on a clustering result after function annotation and clinical characteristics of cervical adenocarcinoma to obtain correlation between gene expression and the clinical characteristics; and on the basis of clustering analysis and correlation analysis results, constructing a deep clustering prediction model based on a variational auto-encoder and a Gamma hybrid model so as to predict the prognosis risk or chemotherapy sensitivity of the patient. The problems that high-dimensional transcriptome data is poor in clustering stability and low in prognosis prediction precision are solved.

The invention discloses a key gene identification method related to tobacco nitrogen response, which comprises the following steps: S1, setting four nitrogen fertilizer gradient treatments on the basis of same phosphorus and potassium fertilization by adopting a field experiment of a completely random block; s2, randomly taking the 6th to 8th leaves of the five plants in each area, and dividing a sample into two parts: quickly freezing one part with liquid nitrogen, and storing at-80 DEG C for RNA (Ribonucleic Acid) extraction and transcriptome sequencing; one part is used for measuring the nitrogen content, and after baking and drying treatment, a KjeltecTM8100 automatic nitrogen analyzer is used for measuring; s3, nitrogen content determination: determining the nitrogen content in the treated sample by using an automatic nitrogen analyzer KjeltecTM8100; according to the method, high-throughput transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) are combined, so that not only can gene expression maps of flue-cured tobacco leaves treated by different nitrogen fertilizers be comprehensively captured, but also gene modules with similar expression modes can be mined from a global perspective.

The invention discloses a flavonoid glycoside glycosyltransferase LbUGT71BX1 in laportea bulbifera as well as the coding gene and the use thereof. The amino acid sequence of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 2. The nucleotide sequence of the coding gene of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 1. According to the invention, on the basis of relevant results of the second-generation transcriptome and the third-generation full-length transcriptome sequencing of the laportea bulbifera, the last-step key enzyme LbUGT71BX1 for the synthesis of the flavonoid glycoside in the laportea bulbifera is screened and identified by using a reverse genetics method, filling the terminal blank of the biosynthesis pathway of the flavonoid glycoside in the laportea bulbifera.

The invention discloses application of a cell surface adhesion molecule CD22 as a target spot in preparation of drugs for treating spinal cord injury related diseases. The change of gene expression in the glial scar formation process is represented by space transcriptome sequencing, and the specific expression of CD22 in the glial scar region is up-regulated. According to single cell sequencing, in-situ hybridization and immunohistochemistry, specific high expression CD22 of part of microglial cells in a glial scar area is found. CD22 is knocked out through a genetic means, and it is found that formation of glial scars after spinal cord injury is remarkably reduced through inhibition of CD22. Behavioral detection finds that the error rate of irregular horizontal ladders is remarkably reduced by inhibiting CD22, and fine movement recovery of hind limbs of mice after spinal cord injury is promoted. The siRNA specifically targeting CD22 is injected into the sheath, so that the expression of microglial cells CD22 is inhibited, the error rate of irregular horizontal ladders of hind limbs can be reduced, and the recovery of the fine movement function of the hind limbs of the mouse after spinal cord injury is promoted. The invention provides a new possibility for development of drugs for spinal cord injury and treatment of spinal cord injury.

The invention relates to the technical field of cotton alkali-resistant gene mining, and particularly discloses a method for jointly screening key genes responding to upland cotton alkali stress based on transcriptome and metabolome, which comprises the following steps: carrying out alkali stress treatment on upland cotton to obtain tissue samples at different time points; performing transcriptome sequencing and metabolome sequencing on the tissue sample to respectively obtain gene expression data and metabolite data; performing difference analysis on the gene expression data and the metabolite data to obtain DEGs and DAMs; carrying out conjoint analysis on the DEGs and the DAMs, and screening to obtain a key gene and a regulatory pathway which respond to alkali stress; analyzing the key gene and the regulatory pathway through a gene expression verification technology to obtain an expression mode; according to the invention, 15 candidate genes related to the alkali resistance of the upland cotton are screened through WGCNA, a theoretical basis is provided for deeply knowing the molecular mechanism of the alkali resistance of the upland cotton, and a new gene resource is provided for subsequent research.

The invention belongs to the field of spatial transcriptome sequencing data processing, and provides a spatial transcriptome spatial domain identification method and system based on a multi-scale neighborhood, and the technical scheme is that based on spatial transcriptome data, graphs of different-scale neighborhoods are constructed, and height variable genes corresponding to the graphs of the different-scale neighborhoods are calculated; generating a mask pattern based on the multi-scale height variable gene and the corresponding spatial position information, extracting to obtain a multi-scale spatial embedding feature based on the mask pattern, and performing adaptive weighting on the multi-scale spatial embedding feature to obtain a spatial embedding feature; extracting a multi-scale gene embedding feature based on the multi-scale highly variable gene, and performing adaptive weighting on the multi-scale gene embedding feature to obtain a gene embedding feature; and splicing the spatial embedding features and the gene embedding features to obtain embedding features, and performing iterative clustering on the embedding features to obtain a spatial domain recognition result. The problem of poor spatial continuity caused by insufficient feature capture is solved, and accurate recognition of the spatial domain is realized.

The invention discloses a preparation method of fresh taxus chinensis stem and leaf protoplasts. The preparation method comprises a preparation process and a purification process. The preparation method of the taxus chinensis stem and leaf protoplast is simple and easy to operate, raw materials are easy to obtain and low in price, reagent components have good biocompatibility, and the taxus chinensis stem and leaf protoplast is free of harmful components, safe and environmentally friendly. The invention provides a simple and rapid taxus chinensis stem and leaf protoplast enzymolysis preparation method, which adopts vacuum filtration, accelerates the permeation of enzymatic hydrolysate and improves the enzymolysis efficiency, so that the protoplast can be rapidly obtained. Meanwhile, high-purity separation of the protoplast is carried out in combination with density gradient sedimentation of the iodixanol solution, so that the protoplast with uniform size and complete form is obtained, and convenience is provided for scientific researches such as subsequent conversion and single cell transcriptome sequencing.

The invention provides a nucleotide mutation site prediction model construction and disease-related point mutation identification method. Specifically, the invention provides a deep learning model-fused nucleotide mutation site prediction model construction method and a disease-related point mutation identification method. According to the method, DNA point mutation and RNA point mutation can be recognized from transcriptome sequencing data in a high-sensitivity and high-specificity mode, and basic data is provided for explaining mutation generation mechanisms and functions on the whole transcriptome and genome level.

The invention discloses an application of burkholderia in root system microorganisms in regulation and control of glycosylation of ginsenoside. The invention provides application of burkholderia or a composition containing the burkholderia in any one of the following applications: 1) regulating and controlling proportions of different glycosylated saponins in ginseng; 2) increasing the proportion of monoglycosylated saponin and / or the proportion of disglycosylated saponin in ginseng; 3) preparing ginseng with a high monoglycosylated saponin ratio and / or a high disglycosylated saponin ratio; and 4) improving ginseng cultivation. In a greenhouse environment, the roots of ginseng seedlings are inoculated with burkholderia, metabolome data shows that the burkholderia regulates glycosylation formation of ginsenoside, and the proportion of monoglycosylated saponin and / or the proportion of disglycosylated saponin can be increased. Further, transcriptome sequencing data analysis shows that the burkholderia regulates and controls the expression of different glycosyl transferases, so that the diversity of glycosylation of ginsenoside is influenced. The burkholderia provides a new thought for refined improvement of a ginseng cultivation technology.

The invention discloses a bisphenol F degradation gene cluster bpf and application thereof. The strain DN12 is separated and can degrade the BPF and grow by taking the BPF as the unique carbon source and energy. On the basis, a BPF degradation gene cluster bpf is obtained from the strain DN12 by adopting whole genome and comparative transcriptome sequencing analysis. Three different degrading enzymes (two-component oxidase BpfAB, monooxygenase BpfC and hydrolase BpfD) are found in the gene cluster and can catalyze gradual degradation of BPF, and a simple aromatic ring compound p-hydroxybenzoic acid is generated.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention belongs to the technical field of biological medicine and molecular biology, and particularly relates to application of circ0005704 and related biological elements thereof in diagnosis and treatment of recurrent spontaneous abortion with unknown reasons. Researches find that differential expression and autophagy level of ULK1 in trophoblast cells of URSA patients are increased. High-throughput transcriptome sequencing is combined with bioinformatics analysis, and it is found that miR-26a-5p is down-regulated in URSA patients, is responsible for up-regulation of ULK1 and promotes autophagy of trophoblast cells, so that occurrence of URSA is increased. Through a high-throughput transcriptional set screening strategy and dual luciferase reporter gene analysis, it is found that circ0005704 enhances the expression of ULK1 through miR-26a-5p. In a word, the research of the invention shows that the circ0005704 / miR-26a-5p / ULK1 signal axis participates in the pathogenesis of URSA by adjusting the migration of the trophoblast cells, and a new target and scientific evidence are provided for the clinical treatment of URSA.

The invention relates to a method for constructing a plasma or serum exosome RNA quality evaluation model suitable for transcriptome sequencing and application of the method, stably expressed RNA candidate factors are screened, a verified primer probe is used for conducting qPCR quantification on exosome RNA, and a Ct value is obtained; an RNACt value of the exosome is associated with RNA-seq data, a reasonable Ct value is screened and set as a reference threshold value, and the RNA quality of the exosome is graded as a measurement index for judging whether the RNA-seq data is qualified or not, so that the quality of miRNA or mRNA of the exosome can be independently evaluated, and the quality of the two RNAs can also be evaluated at the same time. The method can timely and effectively evaluate whether upstream sample collection, exosome separation, RNA extraction and other methods are appropriate or not, can reduce the link of transferring low-quality samples to a downstream high-throughput library construction experiment, and reduces the cost loss of manpower, samples, reagent consumables and the like.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention relates to a training method of a respiratory tract infection disease progress and prognosis prediction model. The training method comprises the following steps: extracting mRNA from peripheral blood of a target patient, and carrying out transcriptome sequencing to obtain a sequencing result; based on the ferroptosis related gene set, comparing ferroptosis score differences of two groups of patients with community-acquired pneumonia and sepsis, and screening corresponding ferroptosis related genes with statistical significance from a sequencing result; screening out genes meeting preset conditions from the ferroptosis related genes based on LASSO regression; and establishing an RTI clinical outcome prediction model through logistic regression by taking whether the patient is sepsis or not as an outcome dichotomy variable and taking the screened gene expression quantity as a prediction variable. According to the invention, after the prediction model is subjected to machine learning screening such as LASSO and the like, the core feature with the highest prediction value is reserved, so that the risk of over-fitting of the model on training data is reduced.

The invention discloses a marker gene composition for identifying and diagnosing white feces syndrome of prawns and application of the marker gene composition. According to the marker gene composition, transcriptome high-throughput sequencing is carried out on intestinal tissues of collected prawns suffering from the white stool syndrome and healthy prawns, and after transcript expression level data is obtained, the marker gene composition capable of being used for identifying and diagnosing the prawn white stool syndrome is obtained by utilizing a random forest algorithm. According to the marker gene composition, transcript expression level data of each gene in the marker gene composition is utilized, a model and a system for identifying and diagnosing the prawn white feces syndrome are also constructed in combination with a random forest algorithm, prawns suffering from the white feces syndrome can be accurately predicted by utilizing the model and the system, and therefore identification and diagnosis of the prawn white feces syndrome are achieved. Based on the marker gene composition, the accuracy of constructing a model and identifying and diagnosing the prawn white feces syndrome by using the marker gene composition is high, the result is stable and reliable, the prevention and treatment of the prawn white feces syndrome are facilitated, and the healthy development of the prawn culture industry is guaranteed.

The invention relates to a molecular typing model construction and identification method of BRCA mutation breast cancer. The method comprises the following steps: performing unsupervised clustering analysis on a gene with most significant change in a common transcriptome expression profile of BRCA1 / 2 mutant breast cancer to obtain an optimal clustering number, and dividing the optimal clustering number into subtypes with different clinical prognosis and molecular characteristics of the optimal clustering number; based on the clustering result, a random forest algorithm is utilized to construct a molecular typing model, the model comprises a plurality of key classification genes, and the molecular typing model is verified through a verification set and an external data set so as to ensure the accuracy, the stability and the clinical applicability of the molecular typing model. Molecular typing can be rapidly and accurately carried out according to common transcriptome sequencing data of a BRCA1 / 2 mutant breast cancer patient tumor sample, and a scientific basis is provided for selection of an individualized treatment scheme.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention belongs to the technical field of frozen slicing, and particularly relates to an animal lung tissue frozen embedding and slicing and space transcriptome sequencing method. In the first aspect, the animal lung tissue freezing and embedding method is provided and comprises the following steps that blood in animal lungs is replaced with first perfusate, and the first perfusate is selected from a first isotonic solution; liquid in the lung is replaced with second perfusate, the perfused lung is obtained, and the second perfusate comprises OCT and a second isotonic solution; and embedding the perfusion lung by adopting OCT (optical coherence tomography), and then quickly freezing. The lung is perfused with the perfusate formed by mixing OCT and the buffer solution, damage to the space structure and form can be reduced, and the tissue slice with the complete internal structure is obtained. Through experimental tests of different thicknesses of perfused lung tissue slices, the slices with the thickness of 10 microns are successfully obtained, in a space transcriptome experiment, the state that most cells belong to monolayer cells is achieved, and the data quality is improved.

The invention relates to a method for screening drought-resistant tea germplasm and application. The method for screening the drought-resistant tea tree germplasm comprises the following steps: step 1, performing repeated drought treatment on a tea tree, and measuring phenotype, metabolome data and transcriptome data of a sample; 2, screening the differential metabolite from the metabolome data, wherein the expression mode of the differential metabolite is that the differential metabolite does not respond or has no significance in response when the differential metabolite is subjected to drought for the first time and has significant response after multiple times of repeated drought, and taking the differential metabolite as a memory metabolite; 3, screening and analyzing differential genes in the tea trees subjected to repeated drought treatment: screening memory key genes from the differential genes; and step 4, according to the transcriptional level of the memory key genes in the tea tree leaves, determining the key genes with high transcriptional level as the drought-resistant tea tree germplasm. The 9 memory key genes are verified, the result shows that the selected gene is consistent with the transcriptome sequencing result, and the gene has good sensitivity, specificity and accuracy in screening drought-resistant tea trees or improving the drought resistance of the tea trees.

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

The invention relates to the technical field of biomarkers, in particular to a biomarker composition for diabetic foot ulcer and application of the biomarker composition. The invention provides a biomarker combination for diabetic foot ulcer. The biomarker combination comprises a DDIT4 gene and a PKM gene. According to the invention, by integrating transcriptome sequencing data and single cell sequencing data, biomarkers DDIT4 and PKM closely related to diabetic foot ulcer (DFU) are successfully screened out. The expression of the two genes in a disease group is remarkably up-regulated, and the two genes are stably expressed in different data sets, so that a reliable molecular target is provided for early diagnosis of DFU.

The invention discloses a screening method of high-specificity variable splicing, which comprises the following steps: by taking transcriptome sequencing data (RNA-seq) of a target group and a control group as an analysis object, carrying out data processing, and then preliminarily detecting and identifying various types of candidate variable splicing events; carrying out multi-dimensional verification on the variable splicing event to obtain a high-credibility event; performing dual verification of multiple data sets on the screened high-credibility events to obtain high-credibility and high-stability events; and carrying out inter-group specific variable splicing screening to finally obtain a high-specificity event. By adopting the screening method disclosed by the invention, the variable splicing events with high accuracy, high inter-group specificity and high data reliability can be obtained, so that the workload of subsequent related research work is reduced.

The invention belongs to the field of biotechnology and medical technology, and discloses application of tumor-associated macrophages with high expression of SLC16A10 in prognosis diagnosis and treatment of colorectal cancer. According to the invention, colorectal cancer single-cell transcriptome sequencing data analysis before and after anti-PD-1 treatment is carried out in the earlier stage; the tumor-associated macrophage subgroup with high expression of the SLC16A10 is enriched in a response group after colorectal cancer anti-PD-1 treatment, and the prognosis of a colorectal cancer patient with high expression of the SLC16A10 is good. Knock-down of the SLC16A10 leads to reduction of expression of the macrophage M1 type marker, and activation and toxicity of co-cultured T cells are reduced. The SLC16A10 promotes T cell activation and weakens immunosuppression on T cells, so that colorectal cancer anti-PD-1 treatment response is caused. The research explains the influence and mechanism of the macrophage SLC16A10 on colorectal cancer anti-PD-1 treatment, and provides a new strategy and theoretical basis for immunotherapy of colorectal cancer.

The present application provides an application of inhibiting or knocking out tobacco polyphenol oxidase gene NtPPO12 in inhibiting tobacco browning. The present application screens an important gene NtPPO12 related to tobacco browning through transcriptome sequencing and molecular biology experiments, further verifies the promoter activity and gene expression site of NtPPO12, and deeply researches the function of the gene through gene editing and overexpression. The present application proves by molecular biology experiments that the NtPPO12 gene provided by the present application is a key gene related to browning in the mature curing process of tobacco, and by regulating the expression amount of the NtPPO12 gene, the browning degree of tobacco can be controlled, and the tobacco maturity and curing resistance can be improved, which is beneficial to reducing the picking frequency, improving the curing characteristics, and improving the appearance and internal quality of tobacco leaves, so it has a broad market application prospect.

The invention discloses a key gene AaMYB114 closely linked with the color character of an actinidia arguta fruit and application of the key gene AaMYB114. The AaMYB114 gene disclosed by the invention is positioned at 5237694-5239966bp of a chromosome 9 of an actinidia arguta genome, and the full length of the AaMYB114 gene is 2273bp. According to the application, all-red type and all-green type actinidia arguta are used as materials for transcriptome sequencing, and the gene AaMYB114 related to the formation of the peel color of the actinidia arguta is explored. Instantaneous conversion of the actinidia arguta fruit proves that the overexpression of the AaMYB114 increases the anthocyanin content in the actinidia arguta peel. Yeast one-hybridization and dual-luciferase report tests prove that the AaMYB114 not only interacts with a promoter of AaGST, activates the expression of the promoter and regulates anthocyanin transport of peel tissues of actinidia arguta, but also is combined with promoters of anthocyanin synthesis structural genes AaPAL, AaCHI and AaF3H and activates the expression of the promoters to promote anthocyanin synthesis. Therefore, the AaMYB114 gene plays a dual regulation and control role in the formation of peel color and luster, and can be used for cultivating red-peel actinidia arguta.

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

The invention relates to the technical field of sequencing of a Stereo-seq space transcriptome, in particular to a coloring agent and application of the coloring agent in sequencing of the Stereo-seq space transcriptome. The staining agent provided by the invention comprises two reagents which are used for staining a cell nucleus and a cell wall respectively, and when the staining agent is applied to tissue chip imaging and / or Stereo-seq space transcriptome sequencing, cellbin data can be quickly provided for plants, manual operation is reduced, the expression data zoning precision is improved, a clustering annotation result is more reliable, and the method has the advantages that the method is simple and convenient to operate, and the staining agent is suitable for large-scale popularization and application in the field of tissue chip imaging and / or Stereo-seq space transcriptome sequencing. A more accurate scientific result is mined; and a basis is established for a plant automation space transcriptome data analysis process.