261 results about "Reference genes" patented technology

The invention belongs to the field of pepper breeding and molecular biology, and discloses a KASP molecular marker closely linked with a pepper fruit color gene CaCHL1, the gene of pepper zunla-1 is used as a reference gene, and the single nucleotide polymorphism at the 13072545th basic group on a pepper chromosome 5 is subjected to basic group G-C replacement. The invention further discloses a primer and a kit for identifying the KASP molecular marker closely linked with the pepper fruit color gene CaCHL1 and application of the primer and the kit in identifying pepper fruit color types or molecular assisted breeding. The screened KASP molecular marker can be directly used for identifying the pepper fruit color, then auxiliary breeding is carried out by depending on the molecular marker, the problems that conventional breeding is long in period and prone to being influenced by the environment can be effectively solved, and the KASP molecular marker has important significance in pepper fruit color breeding practice and research on fruit color change and regulation and control mechanisms.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention belongs to the technical field of gene detection and the field of crop molecular genetic breeding, and particularly relates to a sweet potato gene copy number detection method and application thereof. Specifically, according to the technology, transgenic sweet potatoes or arabidopsis thaliana carrying target genes (exogenous genes such as HPT, 35S, IbMYB1-4 and the like which are cloned to expression vectors, namely external reference genes) are taken as control materials, the control materials and sweet potato cultivated species needing to be analyzed are mixed according to a preset mass ratio, the target genes HPT of the transgenic materials are taken as the external reference genes on the basis of detection of the qPCR technology, and the sweet potato cultivated species needing to be analyzed are obtained. Or IbTUA, IbActin and the like in the screened sweet potato cultivar genome are taken as reference genes, and the relative copy number of the target gene in the genome of a sample to be detected can be quickly obtained by calculating the relative copy number between the target gene to be detected and the reference gene, so that data reference is provided for subsequent genetic research.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The technology disclosed relates to detecting gene conservation and expression preservation. In particular, the technology disclosed relates to detecting gene conservation and epigenetic signals for a reference genetic sequence in comparison to a variant of the reference genetic sequence at base resolution through the generation of a plurality of alternative representations of the sequence in chromatin form which may represent evolutionary conservation, transcription initiation, or epigenetic signals, mapping the plurality of alternative chromatin sequences to a gene expression alterability classifier to generate a gene expression class prediction for the variant, and mapping the alternative chromatin sequence to a pathogenicity predictor to detect pathogenicity of variants.

The invention belongs to the field of pepper breeding, and discloses a KASP molecular marker closely linked with pepper fruit length, a primer, a kit and application, the molecular marker takes a Ca591.0 version gene as a reference gene, the single nucleotide polymorphism at the 70238018 position of the pepper No.3 chromosome is subjected to base A-T replacement, the flanking nucleotide sequence is shown as SEQ ID No: 1, the nucleotide sequence is shown as SEQ ID No: 2, and the nucleotide sequence is shown as SEQ ID No: 3. The sequences of the primers are shown as SEQ ID No: 2-4, and the kit comprises the primers. The invention also discloses an application of the primer or the kit in identification of different length types of pepper fruits or in molecular assisted breeding of pepper fruit length. A primer and a kit are developed aiming at the molecular marker, and can be used for identifying the length of the pepper fruit, so that assistant breeding is carried out by depending on the molecular marker, and the problems that the conventional breeding period is long and is easily influenced by the environment can be effectively solved.

The invention discloses a circulating tumor DNA (deoxyribonucleic acid) standard substance as well as a preparation method and a valuing method thereof. The preparation method comprises the following steps: (1) culturing cells; (2) cell nucleus extraction; (3) carrying out enzyme digestion on cell nucleuses; (4) purifying the fragmented DNA; (5) sampling a sample, and quantifying the sample by nanodrop measurement; (6) sampling the length of a verification fragment; and (7) mixing different ctDNAs according to the finally obtained theoretical mutation abundance of 1%, 5% and 10% to prepare the standard substance. The valuing method comprises the following steps: carrying out digital PCR amplification on a sample through a double-labeled probe to obtain the copy number concentration of a mutant gene and a wild gene or the copy number concentration of a target gene and a reference gene, and calculating the gene mutation frequency and the amplification multiple. According to the method disclosed by the invention, the fragmented DNA with the length similar to that of the ctDNA is obtained; 330bp and large fragments can be removed through two rounds of purification of a paramagnetic particle method, and a single-fragment standard substance is obtained.

The invention discloses a kit for detecting HLA-B27 and a detection method. The kit for detecting HLA-B27 comprises a PCR (Polymerase Chain Reaction) solution, a primer probe mixed solution, a positive reference substance and a negative reference substance, the primer probe mixed solution comprises an upstream primer 1F, a downstream primer 1R and a probe 1P for detecting the HLA-B27, and an upstream primer 2F, a downstream primer 2R and a probe 2P for detecting a reference gene. A fluorescent PCR-melting curve method is adopted, sequence alignment is carried out according to an NCBI database, primers and probes which are high in specificity and capable of simultaneously detecting main subtypes (HLA-B27: 02, HLA-B27: 04, HLA-B27: 05, HLA-B27: 06, HLA-B27: 07 and HLA-B27: 24) of the HLA-B27 are selected, and compatibility detection of different subtypes of the HLA-B27 can be achieved through a pair of primers and a probe. The Tm value in the melting curve instead of the Ct value of the amplification curve is adopted for result judgment, qualitative accuracy is achieved, and the result is visual and easy to interpret.

The invention discloses a method for evaluating the detection effect of a fusion gene detection method, and belongs to the technical field of evaluation based on the detection effect. According to the invention, a detection reference substance I and a detection reference substance II are constructed, and the detection reference substances are connected with a reference gene segment in equal molecular number proportion, so that the detection condition under the condition of equivalent reference gene expression level can be realized, and whether the sample quality reaches the detection standard or not can be deduced; the measurement of the lowest detection quantity of the fusion gene relative to the expression quantity of the reference gene is realized; the first detection reference is used for detecting sensitivity analysis of the fusion type by the detection method of the fusion gene to be detected, and the second detection reference is used for verifying whether a detection result of the detection method of the fusion gene to be detected is a false negative result or not. And the method has a very good application prospect.

The invention discloses a reference gene related to regulation and control of stress-resistant gene expression of arabidopsis thaliana and application of the reference gene, and relates to the technical field of genetic engineering, the reference gene is LHCB5 and / or LHCB4.1, the nucleotide sequence of the LHCB5 is shown as SEQ ID NO.1, and the nucleotide sequence of the LHCB4.1 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the reference gene related to the regulation and control of stress-resistant gene expression of arabidopsis thaliana and application of the reference gene related to the regulation and control of stress-resistant gene expression of arabidopsis thaliana. Various analysis methods are used for analyzing and finding that reference genes LHCB5 and LHCB4.1 show consistent stability under the high-temperature stress condition of arabidopsis thaliana, and when the LHCB5 and the LHCB4.1 are independently used as reference genes and the combination of the LHCB5 and the LHCB4.1 is used as the reference genes for relative quantification, the relative expression quantity of the HSFA2 gene under the high-temperature stress condition is increased and has a consistent trend; it is verified that both the LHCB5 and the LHCB4.1 and the combination of the LHCB5 and the LHCB4.1 can be used as reference genes for qPCR analysis of the arabidopsis thaliana under high temperature stress, a reliable reference standard is provided for gene expression research of the arabidopsis thaliana under high temperature stress in the future, and the accuracy and comparability of experimental data can be improved.

The invention provides a composition, a kit and a detection method for early screening of intestinal cancer. The composition comprises a specific primer and a probe of the KCNQ5 gene, and a reference gene primer and a probe. The detection method is based on sulfite treatment and fluorescent quantitative PCR amplification of cfDNA in plasma, the methylation level of the KCNQ5 gene is judged by calculating the Ct value difference (delta Ct) between the specific primer and the internal reference primer, and methylation is positive when the delta Ct value is smaller than 8.09. The method is easy to operate and non-invasive, healthy people and intestinal cancer patients can be efficiently distinguished, and convenience is provided for early screening of intestinal cancer.

The invention belongs to the field of biotechnology application, and particularly relates to a goose whole genome liquid phase chip and application thereof. The SNP sites on the liquid phase chip are located in a goose reference genome GCA013030995.1, and the number of the SNP sites is 30000. The chip can specifically identify the genetic relationship between commercial geese and local geese, and can also be applied to whole genome association analysis, whole genome selective breeding, target trait QTL positioning analysis, population genetics analysis and the like. According to the invention, by capturing the reference gene and loci in the real sequence thereof, the coverage of sequencing data is improved, and the application value of the chip is enhanced.

The invention discloses a primer and probe combination for detecting a bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR (Polymerase Chain Reaction). The invention further discloses a method for detecting the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR, the bulbus fritillariae cirrhosae specific primers and probes designed based on six bulbus fritillariae cirrhosae-based original plant conserved gene sequences and the 18sRNA universal reference gene primers and probes are adopted, a sample to be tested and a standard reference substance are amplified at the same time in a PCR reaction system, and the specific primers and probes are used for detecting the specific genes of the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR. The method comprises the following steps: qualitatively confirming that a test sample contains the bulbus fritillariae cirrhosae component according to amplification results of a bulbus fritillariae cirrhosae specific primer and a probe, then taking a standard reference substance as a template to obtain two standard curves of a bulbus fritillariae cirrhosae specific gene and a 18sRNA gene, and finally quantitatively analyzing the bulbus fritillariae cirrhosae content in the test sample by adopting a double-marking linear correction method. The method can be used for efficiently, specifically and accurately detecting the ingredients and content of the bulbus fritillariae cirrhosae through PCR.

The present invention relates to methods of treating hematological malignancies, such as acute myeloid leukemia (AML) or spinal dysplasia syndrome (MDS), including hematological malignancies that are refractory to chemotherapeutic agents and / or demethylating agents. Methods involve administering to a patient a CD123x CD3 bispecific binding molecule in an amount effective to stimulate cells that kill the hematological malignancy in the patient. The invention is further directed to embodiments of such methods in which a cell sample from a patient demonstrates the expression of one or more target genes relative to the baseline expression level of such genes, e.g., in a reference population of individuals suffering from hematological malignancies, such as, for example, the expression level of such genes, e.g., in the reference population of individuals suffering from hematological malignancies, or in the reference population of individuals suffering from hematological malignancies. Or the expression level relative to the reference gene is increased.

The invention provides a larimichthys crocea anti-flow character associated SNP marker combination and breeding application, the combination is prepared from a probe of 13 SNP molecular marker combinations for detecting larimichthys crocea muscle elasticity characters, and information of positions, alleles with changed sites and reference genes of all SNP molecular markers on chromosomes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal larimichthys crocea individuals with the anti-flow potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the larimichthys crocea individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. By utilizing the SNP marker combination, the problem that non-lethal detection of the muscle elasticity of the large yellow croaker cannot be realized in a traditional breeding mode can be solved, and smooth development of anti-flow character breeding work of the large yellow croaker can be ensured.

The invention provides a probiotic screening method based on a fine tuning DNABERT model and a convolutional neural network. The method comprises the following steps: acquiring a reference genome sequence and a target sequence sample; preprocessing the reference genome sequence and the target sequence sample; segmenting the preprocessed reference gene sequence, and inputting the segmented reference gene sequence into a DNABERT model for fine tuning training; expanding the number of samples of the preprocessed target sequence, segmenting the sequence by using a sliding window, and inputting the segmented sequence into the fine-tuned DNABERT model for decoding to obtain a representation vector; inputting the representation vector into a CNN model for training, sequencing a target sequence to obtain a to-be-detected sequence sample, encoding the to-be-detected sequence sample, inputting the encoded to-be-detected sequence sample into the CNN model, judging whether the strain is the probiotics or not, and completing screening of the probiotics; according to the method, the data enhancement technology of introducing the reverse complementary sequence and the randomly intercepted sub-sequence is adopted, so that the diversity and the quantity of training data are increased, and the overfitting problem is effectively relieved.

The invention belongs to the technical field of biological detection, and discloses a primer probe composition for detecting CMV virus, a product and application thereof, the primer probe composition for detecting CMV virus comprises a primer probe combination for detecting CMV virus and a primer probe combination for detecting reference gene; the detection technology can be used for detecting the CMV on the DNA level and also can be used for detecting on the RNA level, the detection operation is more flexible, and the detection technology is particularly suitable for rapid safety recheck of cell therapy products and process detection in the production process. The primer probe composition provided by the invention has good detection sensitivity and specificity, is simple to operate, and can be widely applied to CMV detection of cell therapy products.

The invention relates to the technical field of molecular biology, in particular to a reference gene for Chinese olive functional gene expression analysis and application thereof. According to the invention, the reference gene RPN2B which is most stably expressed in fruits of different varieties (lines) of Chinese olives in different development and maturation periods is screened out, and in addition, two reference genes NIFS1 and RPS16 which are only second to the RPN2B are also screened out, so that the method can be used for expression analysis of nutritional quality or other functional genes of the fruits of the Chinese olives of different varieties (lines) in different development and maturation periods; the specificity, the stability and the accuracy of functional gene expression analysis data are remarkably improved, and powerful support is provided for Chinese olive functional gene expression analysis. The invention also provides a special detection primer for detecting the reference gene of the Chinese olive fruit, and the detection primer can quantify the expression quantity and function analysis of the auxiliary gene through fluorescence in the Chinese olive function research, and has high application value.

The present disclosure describes methods, non-transitory computer readable media, and systems that can utilize machine learning models to recalibrate nucleotide base calls (e.g., variant calls) of a call generation model. For example, the disclosed systems can train and utilize a call recalibration machine learning model to generate a set of predicted variant call classifications based on sequencing metrics associated with a sample nucleotide sequence. Leveraging the set of variant call classifications, the disclosed systems can further update or correct nucleotide base calls (e.g., variant calls) that correspond to genomic coordinates, such as multi-allelic genomic coordinates, haploid genomic coordinates, and genomic coordinates that are indicated (by the call generation model) to indicate a homozygous reference genotype.

The application provides a urine exosome-based bladder cancer gene detection system and application, and relates to the technical field of biological detection. Through the cooperative application of the internal reference gene and the diagnosis model, the application provides a reliable endogenous quality control standard for the bladder tissue-derived exosome in the urine exosome sample; meanwhile, the application realizes non-invasive, accurate, rapid screening, diagnosis, prognosis and recurrence monitoring of bladder cancer, lays a key technical foundation and detection tool for the urine exosome-based molecular diagnosis of bladder cancer, and has important clinical transformation value and application prospect.

Provided is an internal reference gene for the real-time quantitative PCR (RT-qPCR) of Yersinia enterocolitica, including one of or a combination of some of glnS, nuoB, glmS, gyrB, dnaK, and thrS genes. Further provided is a primer set for amplifying the internal reference gene for the RT-qPCR of Yersinia enterocolitica. Given that stable housekeeping genes of Yersinia enterocolitica remain unclear, internal reference genes with relatively stable expression at different culture temperatures are selected by screening for Yersinia enterocolitica. Specific detection primers and real and reliable data provide a reference basis for selecting housekeeping genes of Yersinia enterocolitica; moreover, the use thereof alone or in combination, as an internal reference gene, improves the data accuracy, stability, and reliability, thereby solving the problem that usually only empirically selecting 16s rRNA as a single internal reference gene in conventional RT-qPCR results in unstable research results.

The invention discloses a molecular marker combination for detecting adulterated bovine milk in buffalo milk and application of the molecular marker combination. The molecular marker combination comprises a molecular marker 1 and a molecular marker 2, the molecular marker 1 is located at the 2552nd base of a buffalo reference gene sequence NC049568 or the 2540th base of a bovine reference gene sequence AB074968, and the polymorphism of the molecular marker 1 is T or C; the molecular marker 2 is located at the 2555th basic group of a buffalo reference gene sequence NC049568 or the 2543th basic group of a bovine reference gene sequence AB074968, and the polymorphism is G or A. The molecular marker 1 is a molecular marker 1. The molecular marker combination provided by the invention can be effectively used for detecting adulterated bovine milk in buffalo milk.

The invention belongs to the technical field of biological medicines, and particularly discloses a primer probe combination and a kit for detecting genotypes of SMA carriers based on a chromosome level and application of the primer probe combination and the kit. Wherein the primer probe combination comprises a primer probe group for detecting an exon 7 of an SMN1 gene, a probe for detecting an exon 7 of an SMN2 gene, a primer probe group for detecting a short-arm reference gene of a chromosome 5, a primer probe group for detecting a long-arm reference gene of the chromosome 5 and a primer probe group for detecting a reference gene of a human genome. The primer probe combination can eliminate chromosome copy number variation interference, greatly improves the specificity of SMA carrier genotype detection, can simultaneously detect the type I and type II of the SMA carrier genotype, has the detection sensitivity of 99%, and is suitable for large-scale population screening.

The present invention discloses lncRNA internal reference genes for osmanthus hormone treatment, primers, and applications thereof. The present invention obtains the optimal lncRNA internal reference genes for osmanthus under hormone treatment conditions: lnc00239991+18S under ABA treatment, lnc00265419+lnc00249739 under MeJA treatment, lnc00229717+lnc00044331 under ethephon treatment, and lnc00265419+lnc00239991 under multiple hormone treatments. qRT-PCR primers were designed using these genes. This invention fills the technical gap of the lack of internal reference lncRNAs under osmanthus hormone treatment, provides strong support for the quantification of lncRNAs under osmanthus hormone treatment, and provides a theoretical basis for the study of gene function in osmanthus.

The invention belongs to the technical field of forensic medicine, and particularly relates to an mRNA marker combination, a product and application thereof and a forensic human menstrual blood or human menstrual blood spot detection method. The mRNA marker combination comprises a reference gene, a human blood specific mRNA marker and a human menstrual blood specific mRNA marker; the human blood specific mRNA marker is composed of HBA, HBB, ALAS2 and GYPA; the human menstrual blood specific mRNA marker is composed of MMP7, MMP10, SFRP4 and STC1. Standardized identification suggestions are given according to mRNA marker combination expression conditions, the problems of insufficient specificity, flow disorder and the like in the prior art are solved, human menstrual blood or human menstrual blood spots can be accurately distinguished from peripheral blood and other body fluids, cross-platform compatible standardized detection is realized, evidence support is provided for missing cases, and the detection method is suitable for clinical application. The method has important practical significance for improving the forensic physical evidence identification efficiency.

According to the DUX4-IGH rearrangement detection primer probe set, the kit and the application, common rearrangement fracture sites of DUX4-IGH are confirmed, an upstream primer is designed on the upstream of a DUX4 transcript, 33 downstream primers are designed according to IGH fracture positions, a universal primer is designed on the upstream of DUX4, and a universal DUX4 probe is designed near the downstream of the primer; interference between the primer probes is small, influence of non-specific amplification is small, the primer probes are suitable for specific detection of multiple DUX4-IGH rearrangement sites, the problem that rearrangement detection is difficult in the prior art is solved, DUX4-IGH fusion transcripts and ABL1 reference genes can be quantitatively detected in a digital PCR instrument through RT-PCR, a basis is provided for auxiliary diagnosis, and the primer probes have remarkable application prospects.

The invention discloses a detection kit for detecting JAK2V617F mutation, which is characterized by comprising: a JAK2V617F detection reagent, which comprises an inner primer pair, an outer primer pair and a specific probe for specifically amplifying a JAK2V617F mutant gene; the internal reference detection reagent comprises a primer pair and a probe of an internal reference gene; the PCR reaction buffer solution comprises a reaction mixed solution and a HotstartTaq enzyme; the kit comprises a positive control, a known proportion of V617F mutant DNA, a negative control, a JAK2V617F wild type DNA, a positive control and a known proportion of V617F mutant DNA. Compared with the prior art, the invention has the advantage of providing the detection kit for detecting JAK2V617F mutation, which can be used for diagnosis, identification and clinical prognosis of PV.

The invention relates to the technical field of helicobacter pylori multi-drug-resistant gene detection, and discloses a paper sensor-based helicobacter pylori multi-drug-resistant gene detection and treatment quantitative monitoring kit, which comprises an isothermal amplification module, which comprises a loop-mediated isothermal amplification reaction system and is used for carrying out isothermal amplification on 23S rRNA gene and rdxA gene of helicobacter pylori; the paper sensor module comprises a nitrocellulose membrane, the nitrocellulose membrane comprises a test area and a control area, a biotin labeled probe aiming at 23S rRNA gene and rdxA gene mutant amplification products is fixed in the test area, and a biotin labeled probe aiming at reference gene cgt amplification products is fixed in the control area; and the color development module is used for performing color development and quantitative analysis on the detection result of the paper sensor module. The kit disclosed by the invention can be used for detecting various helicobacter pylori multi-drug-resistant genes, can be used for quantitatively monitoring, and is rapid and convenient to monitor.