33 results about "Marker-assisted selection" patented technology

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flowering time trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flowering time trait, and to methods of producing plants having a flowering time trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flowering time trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flowering time trait of interest or for modulating the flowering time of cannabis plants.

This invention relates to the field of biological detection technology, and more particularly to a SNP molecular marker related to the immune characteristics of yaks and its application. The invention provides an SNP molecular marker for detecting the immunoglobulin content in yaks. The SNP molecular marker is located at position 107100737 on chromosome 2 of the yak reference genome LU_Bosgru_v3.0, with a mutated base of A or G. Based on the molecular marker alleles, yaks exhibit three genotypes: AA, AG, or GG. This invention provides a new SNP molecular marker resource for marker-assisted selection of yak immune traits for non-diagnostic purposes, and provides a basis for yak assisted breeding.

The present application relates to the technical field of molecular markers, and particularly relates to a SNP molecular marker related to the anti-oxidative stress capacity of Tibetan sheep and application thereof.A SNP molecular marker related to the anti-oxidative stress capacity of Tibetan sheep, the molecular marker is located at the 196714361th base on the 3rd chromosome of the international sheep genome Oar_v4.0 version, and the mutation base is A or C.Through detecting the base of the 196714361th nucleotide site on the 3rd chromosome of the Tibetan sheep, the anti-oxidative stress index T-AOC content of the Tibetan sheep individual can be judged, and the present application provides a new SNP molecular marker resource for the marker-assisted selection of the anti-oxidative stress trait of the Tibetan sheep for non-diagnostic purposes.

This invention relates to the field of plant molecular genetics breeding technology, and in particular to a SNP molecular marker tightly linked to the major QTL for malic acid in winter melon pulp and its application. The nucleotide sequence of this SNP molecular marker is shown in SEQ ID NO.4, with a G / A mutation at base 26. This molecular marker exhibits tight linkage with malic acid in winter melon pulp; therefore, this invention develops a KSAP primer pair based on this SNP molecular marker. Results from specific embodiments of this invention show that the KASP molecular marker provided by this invention can be used to identify the malic acid content in winter melon seeds or early seedlings with true leaves. This method is highly accurate, low-cost, and time-efficient. Therefore, the SNP molecular marker and KASP primer pair provided by this invention can be used for marker-assisted selection breeding of winter melon, accelerating the process of breeding winter melon flavor and taste quality.

This invention relates to the field of animal genetic engineering technology, providing a molecular marker for the ATP5PO gene associated with bovine sperm motility and its application. The ATP5PO gene molecular marker is located at position 203 of the bovine ATP5PO gene fragment, where an A-G base mutation occurs, resulting in a single nucleotide polymorphism (SNP). The nucleotide sequence of the bovine ATP5PO gene fragment is shown in SEQ ID NO:1. Based on this ATP5PO gene molecular marker, genotype can be determined through sequencing, and the genotype can be associated with normal and thawed bovine sperm motility. This invention utilizes a specific primer set to obtain the bovine ATP5PO gene fragment associated with normal and thawed bovine sperm motility, and uses specific SNP sites within this fragment as molecular markers, providing a theoretical basis and practical application for marker-assisted selection in cattle.

This invention belongs to the field of plant gene map-based cloning and molecular breeding, and relates to the application of the maize gene BRR1 and its functional site in regulating harvest index and yield traits. Through QTL mapping and fine mapping of a maize-Kesury grass population, this invention identified the major-effect QTL-HI1 regulating the harvest index and its key gene BRR1, and discovered that its upstream 9.1 kb insert is an enhancer with H3K27ac modification, which can physically interact with the BRR1 promoter and significantly upregulate gene expression. Knockout of BRR1 leads to a decrease in harvest index, biomass, and yield, and delayed flowering; overexpression of BRR1 increases harvest index and yield by 10–16%. This gene and its functional site are subject to strong selection during domestication and breeding, and can be used through transgenic technology or marker-assisted selection to achieve synergistic improvement of maize harvest index, yield, and flowering time, providing a new approach for high-yield molecular design breeding.

The application belongs to the technical field of animal genetic engineering, and provides a FETUB gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the FETUB gene molecular marker is an SNP site on a FETUB gene fragment of the cow as shown in the sequence table SEQ ID NO:1, specifically, the 315th site of the FETUB gene fragment, the site has an A-G base mutation, resulting in the generation of a single nucleotide polymorphism of the gene. Based on the FETUB gene molecular marker, the genotype is determined by sequence determination, the genotype is associated with the normal and post-thawing vigor traits of the sperm of the cow, and the normal and post-thawing vigor of the sperm of individuals with different genotypes has a significant difference. The FETUB gene molecular marker provided by the application provides a theoretical basis and specific application for marker-assisted selection of the cow.

The application discloses application of an InDel molecular marker related to body height heterosis on chromosome 10 of a pig, a site of the InDel molecular marker is rs712433118, corresponding to an A>ATT mutation at 341376 bp on chromosome 10 of an international pig reference genome 11.1 version, and genotypes of the InDel molecular marker are A / A, A / ATT or ATT / ATT. By using the InDel molecular marker provided in the application for marker-assisted selection breeding, body height traits of the pig can be accurately selected and breeding process of a three-way crossbred pig is accelerated.

The application discloses an application of a SNP marker in LTF gene for selection of colostrum yield traits of sheep. The marker is located in the LTF gene on chromosome 19 of sheep, and the specific SNP marker is a T / C base mutation at the 480th bp in SEQ ID NO: 1 in the sequence listing; the colostrum yield of a sheep individual with a TT genotype is significantly higher than that of an individual with a CC genotype. The application takes sheep as a research object, amplifies a DNA sequence of the 4th exon of the LTF gene of sheep by PCR, analyzes the effect of a specific genotype of the variation site on the colostrum yield of sheep, and accordingly selects and keeps sheep individuals for breeding, so as to rapidly screen excellent individuals with high colostrum yield, accelerate the breeding process of a population with high colostrum yield, and provide a marker resource for marker-assisted selection breeding of colostrum yield of sheep.

The application relates to a method for detecting and / or evaluating milk quality traits of Chinese Holstein cows, and the present application takes IFI47 as a research object, detects genetic variations in a Chinese Holstein cow population, finds that the IFI47 gene exists a SNPs, namely g.40461076 C>G, and exists three genotypes of GG, CG and CC, wherein the dry matter content of the individual with the GG genotype is significantly lower than that of the individuals with the CG and CC genotypes. A solid foundation is laid for subsequent breeding of high-quality cow new strains based on the gene marker assisted selection technology.

This invention belongs to the field of plant molecular breeding technology, specifically relating to a SNP molecular marker related to grape terpenoid aroma substances, its detection method, and its application. The SNP molecular marker is located in the promoter region of the VvGGPPS1 gene, corresponding to a C or T base at position 103 from the 5′ end of SEQ ID NO.1. This invention also discloses primer pairs, detection methods, kits, and applications for detecting this SNP molecular marker. This SNP molecular marker is highly associated with the rose aroma trait in grapes, allowing for early screening during the seedling stage, achieving marker-assisted selection, and significantly shortening the breeding cycle.

The application discloses a biomarker and a detection method for evaluating quality of litchi, and relates to the technical field of biology. The biomarker is a SNP molecular marker, and the nucleotide sequence is shown as SEQ ID NO. 1-3. The molecular marker can be used for evaluating the quality of litchi, especially the evaluation of fructose content. Meanwhile, when the molecular marker is applied to marker assisted selection (MAS) and genomic selection (GS), the genetic improvement progress of litchi varieties can be accelerated.

This invention discloses a SNP marker for the MAP10 gene that affects the number of lambs born in goats and its application. The marker is located in the MAP10 gene on chromosome 28 of goats, specifically at the C / A base mutation at 277 bp of SEQ ID NO:1 in the sequence listing. At this site, goats with the AA and CA genotypes have significantly higher lambing numbers than those with the CC genotype. Using Macheng Black Goats as the research subject, this invention utilizes PCR to amplify the DNA sequence of exon 1 of the goat MAP10 gene. This sequence contains a SNP mutation site. The resulting amino acid changes are analyzed, and the effect of the specific genotype at this site on the number of lambs born in Macheng Black Goats is analyzed. Based on this analysis, Macheng Black Goat individuals can be selected for breeding, which can be used to increase the number of lambs born in Macheng Black Goats and provides marker resources for marker-assisted selection breeding of goat lambing traits.

The present application relates to SNP markers related to tobacco leaf width and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms are conventional SNP types such as A / T, A / G, C / T, C / G or G / T. These SNPs can be typed by conventional molecular detection means, and these sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, which can effectively reflect the genetic influence of leaf width, and can be used for rapid prediction of leaf width of tobacco materials, material screening and molecular marker assisted selection, so as to realize early identification of target leaf width materials, reduce breeding cost and accelerate new strain breeding.

The present application relates to SNP markers related to tobacco leaf length and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T, and these SNPs can be typed by conventional molecular detection means. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, can effectively reflect the genetic influence of leaf length, and can be used for rapid prediction of tobacco material leaf length, material screening and molecular marker assisted selection, so as to achieve early identification of target leaf length material, reduce breeding cost and accelerate new strain cultivation.

This invention discloses a type of Leymus chinensis 1H t This paper discusses S-chromosome-specific KASP molecular markers and their applications, relating to the fields of biotechnology and crop genetics and breeding. The KASP molecular marker is KASP-1H. t The primers for this marker include an upstream primer for the *Elymus styrax* allele, an upstream primer for the wheat allele, and a shared downstream primer, with nucleotide sequences shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively. This marker possesses advantages such as good genetic stability, high specificity, high resolution, and high detection efficiency. It enables high-throughput, automated genotyping without gel electrophoresis, and can accurately detect and track *Elymus styrax* 1H in the complex genetic background of wheat. t The S chromosome. This marker can be widely used in the identification of wheat-Leymus chinensis distant hybrids, chromosome engineering breeding, and molecular marker-assisted selection breeding, providing important technical support for the efficient transfer and utilization of superior genes in Leymus chinensis.

This invention belongs to the field of marker-assisted selection technology for beef cattle, specifically relating to the application of the molecular marker Chr1.S_40470433, which is associated with body height at 6 months of age in beef cattle. The molecular marker described in this invention is a base at locus 40470433 on chromosome 1 of the beef cattle genome GCF_002263795.1_ARS-UCD1.2_genomic.fna. This molecular marker's SNP locus exhibits G / A polymorphism, where G is a favorable allelic variant for body height at 6 months of age in beef cattle. Results from examples show that the body height at 6 months of age in GG and AG genotype populations is higher than that in the AA genotype population, and the GG genotype is also higher than the AG genotype. By detecting the base type at the SNP locus of this molecular marker, it can be used to detect or assist in the detection of the body height trait at 6 months of age in beef cattle, improving the genetic breeding of this trait, accelerating the beef cattle breed selection process, and ultimately improving the efficiency of cattle farms.

This invention belongs to the field of molecular biology technology, specifically relating to a set of SNP marker combinations, probe sets, and their applications for marker-assisted selection of apple single fruit weight. The SNP marker combination consists of 16 SNP markers, including SNP1 to SNP16; the nucleotide sequences of SNP1 to SNP16 are shown in SEQ ID NO.1 to SEQ ID NO.16, respectively. This invention focuses on major variable sites controlling single fruit weight in *Malus* plants, and has developed 16 major SNP markers linked to the apple single fruit weight trait, suitable for marker-assisted selection of apple single fruit weight during the seedling stage of breeding materials.

This invention uses wild-type tobacco *Nicotiana stocktonii*, which is highly resistant to black shank disease race 1, as the starting material. Two co-dominant SSR markers closely linked to the *Nicotiana stocktonii* resistance gene *wBS1_t* were obtained and used to detect the presence of the *Nicotiana stocktonii* resistance gene *wBS1_t* in the tobacco genomic DNA. The SSR markers provided by this invention are rapid, stable, accurate, and low-cost, and can be used as molecular marker-assisted selection for the *wBS1_t* gene in breeding for resistance to *Nicotiana stocktonii* race 1.

This invention relates to the field of agricultural technology, particularly rice. OsSWEET1b Application in increasing anthocyanin content in black rice grains; rice OsSWEET1b The use of genes or vectors to increase the anthocyanin content of black rice grains. OsSWEET1b The gene CDS is shown in Sequence 1 of the sequence listing; a method to increase the anthocyanin content of black rice grains, by... OsSWEET1b Gene overexpression; utilizing the OsSWEET1b gene to regulate anthocyanin synthesis and OsSWEET1b The gene, functioning as a plasma membrane-localized sugar transporter, can simultaneously achieve high anthocyanin content and stable yield, providing a key breakthrough for resolving the "nutritional quality-yield" contradiction in black rice; through overexpression... OsSWEET1b Genes, or their use in marker-assisted selection breeding, can significantly increase the anthocyanin content and yield of black rice grains, thus cultivating new black rice varieties that are "high in nutrition and high in yield".

This invention discloses a method for breeding large-grained, disease-resistant rice. The method includes: hybridization using Yiyang cotton and large-grained rice as the female parent and japonica rice as the male parent; backcrossing the F1 generation with the female parent once; and planting for three consecutive generations to obtain BC1F4. Gs3 For gene mutation detection in the BC1F4 strain, a first primer pair was designed; a second or third primer pair was designed for amplification to determine the large-particle type. Xa3 The mutation site of the gene is identified, and a fourth or fifth pair of primers is designed for amplification to determine resistance; subsequent generations also contain large-particle types. Gs3 Mutant genes) and resistance ( Xa3 The phenotype of the mutant gene. This invention solves the problems of existing technologies not matching the needs of rice cake processing and neglecting the characteristics of local varieties. It applies a breeding method that combines RVA technology, gene marker-assisted selection, and phenotypic judgment, which improves the efficiency of targeted breeding and creates new local specialty rice varieties that are popular with consumers.

The present application relates to SNP markers related to tobacco reducing sugar content and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T, and these SNPs can be typed by conventional molecular detection means. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, and can effectively reflect the genetic influence of reducing sugar content, and can be used for rapid prediction of tobacco material reducing sugar content, material screening and molecular marker assisted selection, so as to achieve early identification of target reducing sugar content material, reduce breeding cost and accelerate new strain breeding.

The application discloses a CRHR1 gene molecular marker for detecting a superovulation trait of a cow and an application method, belongs to the technical field of animal gene engineering, and has the nucleotide sequence length of 666bp of the obtained CRHR1 gene fragment, wherein a c-t base mutation exists at the 455th bp of the segment; the mutation site is used as a molecular marker, genotypes are determined through sequence determination, and the genotypes can be associated with the excellent superovulation trait of the cow. Analysis results show that the superovulation performance of individuals with different genotypes has significant differences. The application uses specific primers to obtain a partial CRHR1 gene segment related to the excellent superovulation trait of the cow, and uses a specific SNP site in the segment as a molecular marker, so as to provide a theoretical basis and specific application for marker-assisted selection of the cow.

The application belongs to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the DHX36 gene molecular marker is a C-G base mutation at the 231st site of a bovine DHX36 gene fragment shown in the sequence table SEQ ID NO:1. Based on the DHX36 gene molecular marker, the genotype is determined by sequence determination, and the genotype is associated with the normal and post-thawing sperm vigor trait of the cow. The analysis result shows that the normal and post-thawing sperm vigor of individuals with different genotypes is significantly different. The application uses specific primers to obtain a bovine DHX36 gene fragment related to the normal and post-thawing sperm vigor trait of the cow, and uses a specific SNP site in the fragment as a molecular marker, thereby providing a theoretical basis and specific application for marker-assisted selection of the cow.

This invention discloses an INHA gene molecular marker related to chicken muscle pH and its application, belonging to the field of genetic engineering technology. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. The mutation site of the molecular marker is located at position 363 bp of the INHA gene CDS sequence, where a C / T mutation exists. Both CC and CT genotypes exist at this mutation site. Experiments have shown that chickens with the CT genotype at the mutation site have significantly higher muscle pH than those with the CC genotype. This invention can provide a theoretical basis for the breeding of meat quality traits in local chicken breeds and for marker-assisted selection.