466 results about "Marker-assisted selection" patented technology

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

Provided are an adenosine triphosphatase sarcoplasmic / endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) gene-based molecular marker for identifying a chicken feed efficiency trait, and an identification method and use thereof. A nucleotide sequence of the molecular marker is shown in SEQ ID NO: 1, and a base at locus 926 in the nucleotide sequence is T or G. In the present application, by identifying a genotype of the molecular marker in a chicken genome and then selecting the chicken feed efficiency trait according to the genotype, a breeding method for early selection of the poultry feed efficiency trait is established. The method is simple, rapid, and low-cost, does not require special instruments, and is suitable for the needs of marker-assisted selection experiments.

The invention discloses a fresh rice seed pre-harvest sprouting resistant OsRDR3 gene and an InDel marker and application thereof, the fresh rice seed pre-harvest sprouting resistant OsRDR3 gene is obtained through a map-based cloning method, and the rice pre-harvest sprouting rate is increased due to deletion of the gene. An inner primer ZIRDR3 of the gene OsRDR3 is designed to be used for rice pre-harvest sprouting resistant molecular marker-assisted selection, the pre-harvest sprouting resistant gene OsRDR3 is successfully introduced into a pre-harvest sprouting susceptible variety wild Xiangxiang B, and the pre-harvest sprouting rate of the variety is extremely remarkably reduced.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention relates to the technical field of molecular markers, in particular to application of an STRN gene in improving the immunity of Tibetan sheep. According to the STRN gene molecular marker related to the immune traits of the Tibetan sheep, the molecular marker is located at the 86920236th basic group on the No.3 chromosome of the international sheep genome Oarv4.0 version, and the mutant basic group is A or C. By detecting the basic group of the 86920236th nucleotide site on the No.3 chromosome of the Tibetan sheep, the content of immune globulins IgA and IgG of a Tibetan sheep individual can be judged, and a new SNP molecular marker resource is provided for non-diagnostic Tibetan sheep immune trait marker-assisted selection.

The invention discloses a primer combination for screening wheat precipitation value and application of the primer combination. The primer combination for screening the wheat precipitation value consists of an upstream primer F1 as shown in SEQ ID NO: 1, an upstream primer F2 as shown in SEQ ID NO: 2 and a downstream primer R as shown in SEQ ID NO: 3. Experiments prove that the primer combination provided by the invention can detect the genotype of to-be-detected wheat based on the C101GSNP site, the C101G SNP site is the 101th nucleotide from the 5'terminal of SEQ ID NO: 4 in a wheat genome, and then the wheat precipitation value character is rapidly and efficiently identified, so that the breeding process of a new variety of high-quality wheat is accelerated. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high precipitation value.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention relates to the technical field of molecular biological detection, in particular to an SNP molecular marker related to immune traits of yaks and application of the SNP molecular marker. According to the invention, the SNP molecular marker related to the immune traits of yaks is obtained through screening and is located at the 55577127th position on the 22 # chromosome of a yak reference genome LUBosgruv3.0 version, and a mutant basic group is G or A. The content of immune globulin A, immune globulin G and immune globulin M of a yak individual can be obtained by utilizing the SNP molecular marker, a new SNP molecular marker resource is provided for non-diagnostic yak immune character marker-assisted selection, and a basis is provided for high-immunity yak breeding.

The invention relates to a pigeon whole genome 5K liquid phase chip and a manufacturing method and application thereof, and belongs to the technical field of molecular biology. According to the pigeon whole genome 5K liquid phase chip disclosed by the invention, the pigeon whole genome 5K liquid phase chip is composed of 5483 SNP molecular markers, and the positions of the SNP molecular markers on a pigeon reference genome Cliv1.0 are as shown in a table 1. Whole genome re-sequencing is carried out on local pigeon varieties and introduced pigeon varieties, quality control screening is carried out through the SNP locus screening strategy to obtain a core SNP locus set, functional SNP loci related to growth and reproductive performance are added, and the method has the advantages of being wide in genetic range, high in pertinence, low in detection cost and high in detection efficiency and has good application prospects. The method has the beneficial effects that the method has important significance on accurate identification and evaluation of pigeon germplasm resources and molecular marker-assisted selective breeding.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a molecular marker related to immune indexes of yaks and application of the molecular marker in breeding. The SNP molecular marker related to the immune traits of the yak is obtained through screening and is located at the 26593056th basic group of chromosome 3 of a yak reference genome LUBosgruv3.0 version, and the mutant basic group is G or A. The content of immune globulin A, immune globulin G and immune globulin M of a yak individual can be obtained by utilizing the SNP molecular marker, a new SNP molecular marker resource is provided for non-diagnostic yak immune character marker-assisted selection, and a basis is provided for high-immunity yak breeding.

The invention belongs to the technical field of beef cattle molecular marker-assisted selection, and particularly relates to application of an SNP molecular marker related to the inclined length character of a beef cattle 6-month-old body. The SNP molecular marker is located at a basic group of a 71085551 site of a No.3 chromosome in a beef cattle genome GCF002263795.1 AR-UCD1.2 genomic.fna, the SNP site of the molecular marker has G / A polymorphism, and A is an unfavorable allelic variation of the oblique length character of the beef cattle at the age of 6 months. By detecting the base type of the SNP site of the molecular marker, the molecular marker is used for detecting or assisting in detecting the oblique length character of the beef cattle at the age of 6 months, genetic breeding of the oblique length character of the beef cattle at the age of 6 months is improved, and the breeding process of beef cattle varieties is accelerated.

The invention belongs to the technical field of molecular biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker combination for single apple remarking assisted selection, a probe group and application of the SNP marker combination and the probe group. The SNP marker combination is composed of 16 SNP markers; the 16 SNP markers comprise SNP1-SNP16, and the number of the SNP markers is 16; the nucleotide sequences of the SNP1-SNP16 are sequentially as shown in SEQ ID NO. 1 to SEQ ID NO. 16. The invention focuses on controlling major variation sites of apple plant single fruit weight, develops 16 major SNP markers linked with apple single fruit weight traits, and is suitable for marker-assisted selection of apple single fruit weight traits in the seedling stage of breeding materials.

The invention discloses a KASP molecular marker for identifying wheat precipitation values in multiple environments and application, and relates to the technical field of biology, in particular to a method for screening or assisting in screening wheat with different precipitation values, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qSV6A.1a or genotype qSV6A.1b, and detecting the precipitation value gt of the wheat with the genotype qSV6A.1b; the precipitation value of wheat of the genotype qSV6A.1a; the wheat of which the genotype is qSV6A.1b is wheat of which the genotype is GG homozygous based on a C101G SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qSV6A.1a is the wheat of which the genotype is CC homozygous based on a C101G SNP (Single Nucleotide Polymorphism) site; the C101G SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO.4 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high precipitation value.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a method for identifying duck palm weight and related molecular marker application thereof, and relates to the technical field of molecular marker-assisted selection, a primer pair is used for amplifying a DNA fragment containing a 63823029th base polymorphic site from the 5'terminal on a fourth chromosome of a duck reference genome IASAASPekinDuckT2T, and the primer pair is used for amplifying a DNA fragment containing the 63823029th base polymorphic site from the 5 '-terminal on the fourth chromosome of the duck reference genome IASAASPekinDuckT2T. The duck reference genome IAASAASPekinDuckT2T is a duck reference genome sequence in a GenBank database, and the duck reference genome sequence is a duck reference genome sequence in the GenBank database; the duck is a Chinese and new white feather meat duck, and the primer pair consists of a DNA (deoxyribonucleic acid) molecule as shown in SEQ ID No.2 and a DNA molecule as shown in SEQ ID No.3. According to the method for identifying the duck foot weight and the application of the related molecular marker, the genotype of the duck can be judged by detecting the genome DNA of the duck, early living screening of the duck foot weight character is achieved, phenotype determination after slaughtering is not needed, the breeding period is remarkably shortened, and the breeding cost is reduced.

The invention discloses a molecular marker for identifying or assisting in identifying soybean oil content and application, and relates to the technical field of biology and application of an SNP (Single Nucleotide Polymorphism) locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 41859817th SNP on a soybean chromosome 5, and the SNP is the 41859817th SNP on the soybean chromosome 5. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is TT homozygosis, the corresponding genotype is A; when the site is GG homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention relates to the technical field of molecular biological detection, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of yaks and application of the SNP molecular marker. According to the invention, the SNP molecular marker related to the immune traits of yaks is obtained through screening and is located at the 163684421th site on a chromosome 1 of a yak reference genome LUBosgruv3.0 version, and a mutant basic group is C or T. The content of immune globulin A, immune globulin G and immune globulin M of a yak individual can be obtained by utilizing the SNP molecular marker, a new SNP molecular marker resource is provided for non-diagnostic yak immune character marker-assisted selection, and a basis is provided for high-immunity yak breeding.

The invention discloses a molecular marker related to the oil content of brassica napus and application of the molecular marker, and belongs to the technical field of molecular marker assisted breeding. A transcription factor BnaA09.MYB52 for coding an MYB structural domain is proved to be capable of influencing the oil content of cabbage type rape seeds, molecular markers corresponding to different haplotypes of the gene and primers of the molecular markers are designed by utilizing re-sequencing data, and the DNA molecular markers can be used for distinguishing the functional haplotypes of the gene of a cabbage type rape strain to be detected; the gene molecular marker can be used for detecting and predicting the oil content of the brassica napus, can also be used for molecular marker-assisted selective breeding of the high-oil-content brassica napus, can effectively accelerate the process of high-oil-content breeding of the brassica napus, is suitable for large-scale popularization and application, and has a wide application prospect.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention belongs to the technical field of molecular biology, and particularly discloses a cotton 10K functional site breeding chip and application thereof. The breeding chip is named as' Cotton Core No.1 ', and comprises a 10K functional site targeting capture probe group designed based on a reference genome of disease-resistant, high-quality and high-yield modern upland cotton No.8, the probe group comprises 11159 SNP (Single Nucleotide Polymorphism) loci, 3981 of the SNP loci are functional loci associated with 13 important agronomic characters, and 7178 of the SNP loci are background loci reflecting genetic diversity; the SNP site information is shown in the specification table 1. The invention discloses a cotton 10K functional site breeding chip and application thereof, the breeding chip contains functional sites with more index function genetic variation, has higher detection accuracy among different cotton varieties, is applied to cotton molecular marker-assisted selection breeding, whole genome selection breeding and whole genome correlation analysis, and has higher detection accuracy. The breeding efficiency can be obviously improved, and the practical application is wider.

The invention discloses an SNP molecular marker related to a pork quality character gene NRG2, a primer pair and application of the SNP molecular marker and the primer pair, and relates to the technical field of pig molecular markers. Wherein the meat quality character is an intramuscular fat content character; the molecular marker is located in a first intron of an NRG2 gene of a second chromosome of a pig, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and g.86Cgt exists at the 86bp position of the sequence; the T basic group is mutated. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig NRG2 gene and the intramuscular fat content character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, namely a new application of the SNP molecular marker in the pig NRG2 gene in marker-assisted breeding of the pork quality character is provided, the early screening of the breeding character of the pig is realized, and the breeding efficiency of the pig is improved. The screening method is simple and rapid.

The invention relates to an InDel molecular marker related to female characters of bitter gourds and application of the InDel molecular marker. According to the invention, an InDel molecular marker related to the strong female character of the bitter gourd is developed, and an amplification primer of the molecular marker related to the strong female character of the bitter gourd is provided, so that the strong female bitter gourd can be quickly and accurately screened in a bitter gourd seed or seedling stage, the auxiliary selection of the molecular marker for female breeding of the bitter gourd is realized, and the breeding efficiency of a female line of the bitter gourd is improved; the method plays an important role in bitter gourd breeding.

The invention relates to the technical field of molecular markers, in particular to a method for identifying the oxidative stress resistance of Tibetan sheep based on SNP molecular markers. The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the oxidative stress resistance of Tibetan sheep, the molecular marker is positioned at the 136620499th basic group on the chromosome 2 of the international sheep genome Oarv4.0 version, and the mutant basic group is T or C. The content of the antioxidant stress index T-AOC of an individual Tibetan sheep can be judged by detecting the basic group of the 136620499th nucleotide site on the second chromosome of the Tibetan sheep, and a new SNP molecular marker resource is provided for non-diagnostic auxiliary selection of the antioxidant stress character marker of the Tibetan sheep.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention provides a Sichuan local chicken 11K SNP liquid phase chip and application thereof, the liquid phase chip is prepared from a Sichuan local chicken 11K SNP molecular marker combination, the Sichuan local chicken 11K SNP molecular marker combination is composed of 11747 SNP molecular markers, and chromosome positions and numbers of the 11747 SNP molecular markers on a chicken reference genome GRCg7b are shown in a specification table 1. The chip is high in detection flux, high in target site detection rate (99.91%) and accurate and reliable in typing result (the repetition consistency rate is 99.82%), and is a molecular marker chip with low density, high accuracy and low cost; the method can be used for chicken germplasm resource identification and improvement, genetic diversity evaluation, population structure analysis, genetic relationship identification, transmission map construction and QTL positioning, whole genome association analysis, molecular marker-assisted selection, whole genome selective breeding and the like.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention belongs to the field of rice genetic breeding, and discloses a breeding method of high-quality disease-resistant large-grain two-line sterile line rice, which ingeniously fuses two major technical fields of molecular biology and rice genetic breeding. A two-line sterile line A with an excellent genetic background is selected as a female parent and a restorer line B is selected as a male parent for hybridization, and then individuals carrying target disease-resistant and grain weight genes are accurately screened by using a molecular marker-assisted selection technology. By combining strict resistance inoculation test and appearance quality evaluation, the bred sterile line not only has excellent disease resistance (resistance to rice blast and bacterial blight), but also has large grain shape and high appearance quality. In addition, through multi-generation shuttling and generation-adding propagation, the hereditary character is further stabilized. The breeding method disclosed by the invention is efficient and accurate, provides a new thought and technical means for the field of rice genetic breeding, and is of great significance for improving the yield and quality of rice.