211 results about "Marker-assisted selection" patented technology

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention discloses a method for identifying duck palm weight and related molecular marker application thereof, and relates to the technical field of molecular marker-assisted selection, a primer pair is used for amplifying a DNA fragment containing a 63823029th base polymorphic site from the 5'terminal on a fourth chromosome of a duck reference genome IASAASPekinDuckT2T, and the primer pair is used for amplifying a DNA fragment containing the 63823029th base polymorphic site from the 5 '-terminal on the fourth chromosome of the duck reference genome IASAASPekinDuckT2T. The duck reference genome IAASAASPekinDuckT2T is a duck reference genome sequence in a GenBank database, and the duck reference genome sequence is a duck reference genome sequence in the GenBank database; the duck is a Chinese and new white feather meat duck, and the primer pair consists of a DNA (deoxyribonucleic acid) molecule as shown in SEQ ID No.2 and a DNA molecule as shown in SEQ ID No.3. According to the method for identifying the duck foot weight and the application of the related molecular marker, the genotype of the duck can be judged by detecting the genome DNA of the duck, early living screening of the duck foot weight character is achieved, phenotype determination after slaughtering is not needed, the breeding period is remarkably shortened, and the breeding cost is reduced.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of male semi-fine wool sheep and application of the SNP molecular marker. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of elephant male semi-fine wool sheep. The SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of a Lanblet sheep reference genome ARS-UIRambv3.0, GCF016772045.2, and the SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of the Lanblet sheep reference genome ARS-UIRambv3.0; the mutant basic group is G or T. By detecting the basic group of the 24648039th nucleotide site on the fifth chromosome of the elephant male semi-fine wool sheep, the content of immune globulins IgA, IgG and IgM of an elephant male semi-fine wool sheep individual can be judged, and a new SNP molecular marker resource is provided for non-diagnostic elephant male semi-fine wool sheep immune character marker-assisted selection.

The invention discloses an application method of using an ERC1 gene as a cattle superovulation character molecular marker, and belongs to the technical field of animal character detection gene engineering. The length of the nucleotide sequence of the ERC1 gene segment is 520bp, and a c-t base mutation exists at the 293bp position. The mutation site is used as a molecular marker, the genotype is judged through sequence determination, the genotype can be associated with the excellent ovulation character of the cattle, and the ovulation performance of individuals with different genotypes is remarkably different. According to the invention, a specific primer is utilized to obtain an ERC1 gene part segment related to the excellent superovulation character of cattle, and a specific SNP site in the segment is taken as a molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention belongs to the field of biotechnology and livestock breeding, and relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep meat quality traits, a primer group, a kit and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and Ggt exists at the 46th bp position of the sequence as shown in SEQ ID NO.4; a is a mutation site. By rapidly and accurately detecting the SNP marker related to the meat quality traits of the'Auodurou Lake 'sheep genetic resource population, the'Auodurou Lake' sheep variety dominant genetic resource population can be rapidly established, so that the marker-assisted selection breeding process of the sheep meat quality traits is accelerated.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located at the 6293801th basic group on the No.15 chromosome of a goat, the specific SNP marker site is C / G basic group mutation at the 392bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of a CC genotype goat individual and a CG genotype goat individual at the site is remarkably higher than that of a GG genotype individual. The method comprises the following steps: by taking a Muncheng black goat as a research object, carrying out PCR (Polymerase Chain Reaction) amplification on a segment of ENSCHIG00000009743 gene, wherein the segment contains a goat 15 # chromosome g.6293801Cgt; according to the present invention, the DNA sequence of the G mutation site is analyzed, the influence of the specific genotype of the site on the lambing number of the Mancheng black goats is analyzed, and the individual breeding of the Mancheng black goats is performed according to the influence, such that the Mancheng black goat lambing number can be increased, and the breeding of the new variety (strain) using the Mancheng black goats as the breeding material can be accelerated; and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The application discloses a G101T SNP related to wheat wet gluten content, which is located at the physical position 80813538 bp of the 6A chromosome in the Chinese spring wheat genome IWGSC RefSeq v1.0, and the genotype is G or T. The application develops a KASP marker and a KASP primer group for identifying the wheat wet gluten content based on the SNP site, and provides a method for identifying or assisting in identifying the wheat wet gluten content, and it is found through the method that the wheat wet gluten content of the G101T SNP site genotype TT is higher than or higher than the wheat wet gluten content of the genotype GG. The application has important theoretical significance and economic value for utilizing molecular marker assisted selection to obtain wheat germplasm or breeding offspring materials with higher wet gluten content.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant comprising a quantitative trait locus (QTL) or a causal polymorphism associated with a flower density trait, and to Cannabis spp. plants having a flower density trait of interest comprising defined allelic states of polymorphisms defining the QTL or defined allelic states of causal polymorphisms provided herein. Also provided are Cannabis spp. plants with a flower density trait of interest comprising defined allelic states of polymorphisms and plants identified, characterized or produced by the methods described. Further provided are methods of marker assisted selection, genomic selection, marker assisted breeding, and genetic modification, for obtaining plants having a flower density trait of interest.

The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and / or Ant2 gene allowing color sorting of seeds.

The present disclosure provides methods and compositions for producing, detecting, and selecting soybean plants and seeds comprising at least one high protein CCT (CONSTANS, CO-like and TOC1) domain containing variant allele and introgressing the high protein CCT variant allele into soybean plants. The present disclosure also provides methods and compositions for producing, detecting, and selecting soybean plants producing seeds having a high protein content including breeding methods for introgressing high protein alleles into soybean plants using marker assisted selection using markers linked to or associated with high protein CCT in soybean.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

The invention provides a molecular marker used for Tibetan sheep marker-assisted selection and related to disease resistance characters and application of the molecular marker, and belongs to the technical field of molecular marker-assisted breeding, the molecular marker is located at the 29402282th basic group on the 10th chromosome of the international sheep reference genome Oarv4.0 version; the variation type is T / C, the molecular marker site has three genotypes of TT, TC and CC, the genotype of the molecular marker site has significant correlation with the content of cytokines in a Tibetan sheep blood sample, and the content of IL-1alpha, IL-1beta, IL-6, TNF-alpha, IL-1Ra and IL-10 of a Tibetan sheep individual can be judged by detecting the genotype of the SNP molecular marker. The SNP molecular marker can be used for non-diagnostic Tibetan sheep immune trait marker-assisted selection, and a new SNP molecular marker resource is provided for early molecular breeding of Tibetan sheep.

The invention relates to the technical field of molecular markers, in particular to a disease-resistant character related molecular marker on a Tibetan sheep chromosome 15 and application. The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to disease resistance characters of Tibetan sheep. The SNP molecular marker is positioned at the 72758424th basic group on a No.15 chromosome of an international sheep reference genome Oarv4.0 version; the mutation basic group is A or G. The contents of IL-1alpha, IL-1beta, IL-6, TNF-beta, IL-1Ra and IL-10 of a Tibetan sheep individual can be judged by detecting the basic group of the 72758424th nucleotide site on the No.15 chromosome of the Tibetan sheep, and a new SNP molecular marker resource is provided for non-diagnostic Tibetan sheep immune trait marker-assisted selection.

The application discloses a KASP molecular marker of watermelon peel flower spot traits and application thereof. The molecular marker ClRP obtained by the application is a KASP marker of a watermelon low-temperature-resistance response control leaf area change gene, and can be used for identifying the auxiliary breeding of the watermelon peel flower spot traits. A peel flower spot material ZJU129 and a peel stripe material ZJU132 are crossed, and then the marker assisted selection is combined. The application can predict the peel appearance phenotype at the seedling stage, reduce the workload of field cultivation, and greatly improve the screening accuracy and efficiency.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located on a PIEZO1 gene on a capa circus 18 # chromosome, the specific SNP marker site is A / C base mutation at the 236bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of CC genotype and AC genotype goat individuals at the site is remarkably higher than that of AA genotype individuals. According to the present invention, the Musheng black goats are adopted as the research object, the DNA sequence of the 33rd exon of the PIEZO1 gene is subjected to PCR amplification, the sequence has an SNP variation site, the influence of the site polymorphism on the goat lambing number is analyzed, the individual Musheng black goat breeding is performed according to the influence, and the method can be used for improving the Musheng black goat lambing number so as to provide the basis for the breeding of the Musheng black goats. The breeding of a new variety (strain) taking the Muscheng black goat as a breeding material is accelerated, and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The invention discloses a method for cultivating a high-yield and gibberellic-disease-resistant wheat strain. According to the method, a dwarf-male-sterile wheat population is obtained by hybridizing backbone parent dwarf-male-sterile wheat and a gibberellic disease-resistant wheat variety carrying an Fhb1 gene, then a dwarf, large-ear and high-yield wheat variety (line) and excellent sterile plants in the dwarf-male-sterile wheat population are backcrossed or hybridized, and an Fhb1 gene molecular marker-assisted selection and haploid breeding technology is combined. A homozygous strain carrying the Fhb1 gene is rapidly obtained, and finally the high-yield and gibberellic-disease-resistant new wheat strain is obtained through gibberellic-disease resistance and main agronomic-character phenotype identification. Aiming at the bottleneck of incoordination of wheat high yield and gibberellic disease resistance, the invention develops the advantage of convenience in population expansion of dwarf male-sterile wheat, the advantage of improvement of selection efficiency by molecular marker-assisted selection and the advantage of shortening of breeding cycle by haploid technology, and provides a new idea for high-yield gibberellic disease-resistant breeding.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention provides an SNP (Single Nucleotide Polymorphism) marker primer pair related to pig multirib traits and application of the SNP marker primer pair. The SNP site is a nucleotide site of chromosome 7 rs701714758 of a reference sequence of an international pig genome version 11.1, has G / A polymorphism, is located in a protein coding region of an ABCD4 gene, and is remarkably associated with rib number characters of Duroc pigs, landrace pigs, large white pigs and Pietrain pigs through verification of whole genome association analysis and meta analysis. The invention further provides a primer pair for detecting the SNP marker, an upstream primer of the primer pair is SEQ ID NO: 2, and a downstream primer of the primer pair is SEQ ID NO: 3. The SNP marker can be applied to marker-assisted selection and whole genome selection of rib number characters of multiple varieties of pigs, can accurately screen a multi-rib number pig group, improves character prediction accuracy, increases pork and rib row yield, meets consumption demands, and has both social and economic benefits.

The invention provides an SNP molecular marker related to disease resistance characters of Tibetan sheep, a detection method and application of the SNP molecular marker, and belongs to the technical field of detection of disease resistance characters of Tibetan sheep. The SNP molecular marker is located at the 64702329th basic group on the No.3 chromosome of an international sheep reference genome Oarv4.0 version; the content of IL-1alpha, IL-6, TNF-alpha, TNF-beta and IL-1Ra of a Tibetan sheep individual with the genotype of GG at the site of the SNP molecular marker is remarkably lower than that of a Tibetan sheep individual with the genotype of GA and AA, and the content of IL-10 of a Tibetan sheep individual with the genotype of AA is remarkably lower than that of a Tibetan sheep individual with the genotype of GG. According to the genotype of the SNP molecular marker site, the content of key cell factors in Tibetan sheep individuals can be judged, and a new SNP molecular marker resource is provided for Tibetan sheep immune trait marker assisted selection.

The invention relates to the field of plant genetic breeding, and discloses an SNP molecular marker related to corn drought resistance and application of the SNP molecular marker. Two markers ZMDRI291441904 and ZMDRI159118475 which are obviously associated with the corn drought tolerance index are mined through whole genome association analysis, the marker sites are A / G and T / C respectively, the two markers are used for performing genetic typing on the corn inbred line, and the drought tolerance indexes of the corn inbred line of the two genotypes of each marker respectively follow the following conditions: GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, , AA, CCgt; tT. Experiments show that the maize inbred line with relatively high drought tolerance index can be found by detecting the SNP site. The invention provides a new method for corn molecular marker-assisted selective breeding, and has important significance in drought-resistant screening of corn inbred lines and breeding of drought-resistant new varieties.

The disclosure discloses a primer for amplifying a molecular marker of powdery mildew resistance gene PmDR776 of Triticum dicoccon Schrank, where the molecular marker primer includes a forward primer HENU654-F and a reverse primer HENU654-R; the nucleotide sequence of the forward primer HENU654-F is shown in SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU654-R is shown in SEQ ID NO:2. The molecular marker primer provided by the disclosure is applied to detection and identification of gene PmDR776 and auxiliary identification of a wheat powdery mildew resistance trait, and molecular marker-assisted selection breeding.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flowering time trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flowering time trait, and to methods of producing plants having a flowering time trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flowering time trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flowering time trait of interest or for modulating the flowering time of cannabis plants.

The invention discloses an STS marker related to the number of adventitious roots of wheat and application of the STS marker. The invention provides an application of an InDel molecular marker, the InDel molecular marker is a DNA fragment from the 112th site to the 128 site of which the nucleotide sequence is SEQ ID NO: 1, the application is to identify or assist in identifying the number of adventitious roots of wheat, and the InDel molecular marker can be used for wheat breeding. The STS molecular marker provided by the invention can provide a strong selection site, improve the selection accuracy of a breeding material with a large number of adventitious roots, and realize the target of molecular marker-assisted selection of an excellent root system.

The invention provides an SNP (Single Nucleotide Polymorphism) marker for influencing the disease resistance character of Tibetan sheep and application of the SNP marker, and belongs to the technical field of molecular biology detection. The SNP molecular marker is located at the 190163785th basic group on the first chromosome of an international sheep reference genome Oarv4.0 version; the IL-1alpha, IL-1beta, TNF-beta and IL-1Ra of the Tibetan sheep individual with the genotype of GG are obviously lower than those of the Tibetan sheep individual with the genotype of TT or TG, and the IL-10 of the Tibetan sheep individual with the genotype of GG is obviously higher than those of the Tibetan sheep individual with the genotype of TT or TG. The content of IL-1alpha, IL-1beta, TNF-beta, IL-1Ra and IL-10 of a Tibetan sheep individual can be judged by detecting the genotype of the SNP site of the Tibetan sheep, and a new SNP molecular marker resource is provided for non-diagnostic Tibetan sheep immune trait marker-assisted selection.