932 results about "Marker-assisted selection" patented technology

This invention provides methods for monitoring QTL effects and marker assisted selection (MAS) involving providing a recursively determined correlation between one or more markers and a phenotype of interest.

The present invention provides methods of marker assisted selection for high oleic / low linolenic traits in canola and in other oil seed crop species, as well as isolated nucleic acids for use as molecular markers in such methods. In particular, molecular markers and Brassica nucleic acid corresponding to fad2 and fad3 gene mutations are disclosed. The markers of the present invention are highly useful for the direct selection of desirable fad2 and fad3 alleles during marker-assisted trait introgression and breeding. In a one aspect of the embodiment, two single nucleotide polymorphism (SNP) markers are provided which correspond to the alleles. Thus, the present invention advantageously permits one of skill in the art to breed for the molecular markers described herein, or derivatives thereof, rather than breeding for a high oleic / low linolenic phenotype.

The invention overcomes the deficiencies of the prior art by providing methods for marker assisted selection to create plants of a soybean variety that exhibit a mid / low linolenic acid content with a commercially significant yield and an agronomically elite phenotype. The invention also provides derivatives and plant parts of these plants. Further provided by the invention are methods for the use of these plants. The invention is significant in that oil with decreased linolenic acid exhibits numerous beneficial characteristics yet prior art varieties with decreased linolenic acid also exhibited decreased yield and poor agronomic quality.

The invention provides a method of introgressing soybean sudden death syndrome (SDS) or soybean cyst nematode (SCN) resistance into non-resistant soybean germplasm. Loci associated with SDS resistance or with SCN resistance in soybean lines known to be resistant to SDS or to SCN are used in marker assisted selection during introgression of SDS or SCN resistance into elite germplasm. In addition, the method may be used to confirm selection of resistance in new soybean cultivars.

The invention belongs to the field of rape molecular breeding, and relates to preparation of specific molecular markers of related genes MINI3 and TTG2 of the brassica napus grain weight. Double haploid colony (DH) is constructed with brassica napus I A 254 as a female parent and a brassica napus I A 177 as a male parent through hybridization, and the DH colony genotype and the thousand seed weight data are analyzed to obtain a QTLs locus of grain weight character. The MINI3 and the TTG2 genes of the IA254 and the IA177 are cloned by using a homology based candidate gene method, specific molecular markers MINI3a and TTG2a of the MINI3 and the TTG2 genes are designed according to sequence different locuses, and the molecular markers MINI3a and TTG2a are located on two grain weight QTLs locus of an A5 linkage colony for related verification and application, which proves that the molecular marker prepared by the invention is a novel genetic marker. The gene sequence is obtained firstly. The invention provides a novel marker for the molecular breeding of the brassica napus grain weight, and also provides useful information for candidate gene clone and marker auxiliary selection of thethousand seed weight character locuses of the brassica napus.

The invention overcomes the deficiencies of the prior art by providing methods for marker assisted selection to create plants of a soybean variety that exhibit a mid / low linolenic acid content with a commercially significant yield and an agronomically elite phenotype. The invention also provides derivatives and plant parts of these plants. Further provided by the invention are methods for the use of these plants. The invention is significant in that oil with decreased linolenic acid exhibits numerous beneficial characteristics yet prior art varieties with decreased linolenic acid also exhibited decreased yield and poor agronomic quality.

The invention discloses an SSR molecular marker A002 for identifying males and females of kiwi fruit hybrid populations, relating to the technical field of molecular genetic breeding. The male plant specific fragment nucleotide sequence of the molecular marker A002 is shown in SEQ.ID No.1; the female plant specific fragment nucleotide sequence is shown in SEQ.ID No.2; and the primer sequence is shown in SEQ.ID No.3 and SEQ.ID No.4. The molecular marker A002 can be used for the early identification detection of males and females of kiwi fruits so as to avoid the great waste caused by abundant male plants in the breeding and production; by utilizing a high-density genetic map, sex related molecular markers can be rapidly explored to assist the breeding and important practical experience is provided for the development of the functional markers; and being used for marker assisted selection of males and females, the molecular marker A002 is capable of greatly improving the selection efficiency of the female plants in the breeding and shortening the breeding period, and has great application potential and relatively high economic value.

The invention provides a method for cultivating a novel Dingyuan pig breed, which relates to a method for cultivating a novel pig breed. The invention provides the method for cultivating the novel Dingyuan pig breed which has the advantages high fecundity, good meat quality and strong stress resistance of Dingyuan pigs as well as the characteristics of fast growth, high lean meat percentage and the like of imported pigs. The method comprises the following steps: a Dingyuan sow is hybridized with a novel USA strain Duroc boar to cultivate a first generation sow core group; second grading hybridization is performed to the first generation sow core group and a Dingyuan boar or the novel USA strain Duroc boar; from the third generation, group closure propagation and breeding are adopted respectively, and crossing of two to three generations is performed; and selection and remaining are performed through methods of comprehensive selection indexes and molecule marking assistance selection to form the novel Dingyuan pig breed, namely a novel breed of Wan Huai pigs and a novel breed of Dingyuan-Duroc pigs.

The invention discloses a major QTL (Quantitative Trait Locus) of a cotton high-strength fiber and a molecular marker and application thereof. The major QTL of the cotton high-strength fiber is linked with two SSR (Simple Sequence Repeat) markers, is positioned through markers NAU / SSR / FS1195 and JESPR / SSR / FS2127 and is 0.01-2.39 centimeters away from a marker NAU3700; and the major QTL locus can explain 4.51%-17.55% of phenotype variation and achieves the LOD (Limit Of Detection) value between 3.23 and 7.09. The major QTL disclosed by the invention overcomes the defects of high cost, high difficulty and slow progress of quality breeding in the prior art, greatly increases the selection efficiency of the high-strength fiber by utilizing the major QTL locus of a cotton high-strength fiber gene and the molecular marker thereof, can be used for exploring high-quality fiber gene resources, provides the gene resources and a marker auxiliary selection technology for the quality breeding of cotton and can be applied to the production and quality detection of high-strength fiber cotton varieties.

The invention discloses a method for quickly detecting a cattle MLLT10 gene CNV marker and application of the method. Based on the real-time fluorescent quantitation PCR technology, cattle genome DNAserves as a template, the cattle MLLT10 gene copy number variation area is amplified by using specific PCR primers, and partial fragments of the cattle BTF3 gene are amplified to serve as an internalreference, and finally, by using the 2-delta delta Ct method, the individual copy number variation type is computed and determined. The method lays a basis for the relationship between the cattle MLLT10 gene copy number variation and the growth trait, the detection method is simple and fast, the method can be used for accelerating cattle marker-assisted selection breeding work, and application andpopularization are facilitated.

The invention belongs to the technical field of rape molecular breeding, in particular relates to a preparation method for a cabbage type rape high oleic acid codominant SCAR molecular marker and application as marker assisted selection in breeding high oleic acid cabbage type rape new products. The difference of nucleotide sequences of AA254 and AA177 is identified by cloning and checking sequences on DNA segments of fad2 gene of genomes of high oleic acid cabbage type rape strain AA254 and low oleic acid cabbage type rape strain AA177, primers YQ-Fad2a-1 and YQ-Fad2a-2 are developed by utilizing the difference of the nucleotide sequences provided by the invention, and then the codominant SCAR molecular marker which can distinguish cabbage type rape high oleic acid and low oleic acid is obtained. The invention provides a new marker for the rape molecular breeding. The invention also discloses the preparation method and the application of the molecular marker.

The invention overcomes the deficiencies of the art by providing an agronomically elite soybean plant with non-transgenic mutations of at least two of the glycinin subunits selected from the group consisting of Gy1, Gy2, Gy3, Gy4, and Gy5, such as conferring a Gy3 and Gy4 null phenotype and increased β-conglycinin content in seed. The invention also provides derivatives, and plant parts of these plants and uses thereof. Methods for marker assisted selection of soybean varieties comprising non-transgenic mutations conferring a reduced Gy1, Gy2, Gy3, Gy4, and Gy5 phenotype are also provided as part of the current invention. Methods for producing such plants that are further lipoxygenase and / or Kunitz Trypsin Inhibitor null and the plants produced thereby are also provided. The invention is significant in that soybeans from such plants are preferred dietary additives and provide important health benefits.

The present invention relates to methods for identifying cucumber lines having increased resistance to Downy Mildew, and identification of genetic markers linked to gene(s) conditioning such increased disease resistance. The present invention also relates to methods of breeding cucumber plants from lines having increased Downy Mildew resistance by marker-assisted selection, compositions including nucleic acid probes or primers which are useful for such marker assisted selection, and plants and plant parts produced by such methods.

The invention discloses a method for efficient selective breeding of a new odor type rice blast resisting rice variety. The method is characterized in that eurytopic and high-quality odor type rice restorer fengxianghui No. 1 is taken as a receptor parent, a rice blast resisting rice material containing rice blast resisting genes is taken as a donor parent, the conventional breeding technique is adopted, the high-throughput molecular marker-assisted selection technique, the rice anther in vitro culture technique and the human nose fragrance smelling and resistance field identification means are comprehensively utilized, a resistant improved odor type rice fengxianghui No. 1 restorer with stable fragrance, high rice blast resistance and excellent agronomic trait is prepared, and the odor type rice blast resisting rice variety is obtained through efficient selective breeding by means of combination and measuring matching of the resistant improved odor type rice fengxianghui No. 1 restorer and a sterile line. The method is efficient, quick and low in cost. Meanwhile, the traditional breeding technique and the modern molecular biological technique are effectively combined, the popularization and application values of the variety obtained through selective breeding are high, and the method can meet the requirement of enterprises for commercial breeding.

The invention discloses a primer pair for assaying the thousand-grain weight of wheat and a related molecular marker. The specific primer pair disclosed by the invention consists of a single-stranded DNA fragment shown in a sequence 3 of a sequence table and a single-stranded DNA fragment shown in a sequence 4 of the sequence table. The invention discloses allelic variation sequences, namely TaGS-D1a and TaGS-D1b, of an ordinary wheat gene TaGS and provides functional markers for assaying the allelic variation of the TaGS-D1a and the TaGS-D1b and the relationship between the functional markers and the thousand-grain weight of the wheat. Through applying the functional markers provided by the invention to molecular-marker-assisted selection, wheat varieties (materials) with higher thousand-grain weight can be quickly screened out, and thus, the pace for breeding new high-yielding wheat varieties is accelerated. The primer pair and the related molecular marker have important theoretical significance and economic value in the molecular-marker-assisted selection of the high-yielding wheat varieties.

The invention discloses a KASP marker relative to the wheat root system character and application of the KASP marker. A special primer for the KASP marker can be used for identifying whether a genotype of wheat to be tested is TT, CC or CT and can determine the superficial area of the root system according to the genotype of the wheat to be tested; and the superficial area of the root system of the TT-genotype wheat to be tested is larger than the superficial area of the root system of CT or CC-genotype wheat to be tested, so that the KASP marker can be used for screening wheat varieties withexcellent root system character, lays the theoretical foundation for breeding the wheat varieties with high and stable yield and good quality and provides a marker assisted selection means.

The invention belongs to the molecular biology technology field, and concretely relates to a method for sequencing and development of Asplenium nidus L. EST-SSR primers based on transcriptome. The method comprises the following steps: firstly, a transcriptome library is constructed; secondly, transcriptome data is obtained, namely, sequencing data is subjected to splicing by utilization of a Trinity software and sequences are spliced into a complete transcriptome; thirdly, SSR site search is carried out, namely, combined with a Perl programming language method, a lot of sequence information is subjected to batch processing, and SSR site search is carried out; fourthly, batch design of EST-SSR primers is carried out. Different fiddlehead materials are selected to verify the designed SSR primers, if strips are detected, successful SSR primers are obtained. Through the method, 4063 pairs of SSR primers are designed, and a new method and thought are provided for development of Asplenium nidus L. EST-SSR primers and further for achieving molecule label auxiliary selection breeding.

The invention belongs to the technical field of screening of molecular markers of boars, and particularly relates to a molecular marker relative to sperm viability and a total sperm number of a boar and application. The molecular marker is cloned from a gene segment with a login number of ALGA 0001958. Through a gene chip technology, a gene is typed, screening is conducted to obtain the molecularmarker relative to characters of the sperm viability and the total sperm number of the boar, a marked nucleotide sequence is as shown in SEQ ID NO:1, A / G allele mutation exists in a base position of asite 101 of the sequence, and when nucleotide of the site 101 on the nucleotide sequence as shown in the SEQ ID NO:1 is A, the fact that the boar has higher sperm viability and a larger total sperm number is judged. The invention discloses a screening method of a molecular marker relative to a boar seminal fluid character and correlation analysis thereof. New SNP marker resources are provided formarker assisted selection of the characters of the sperm viability and the total sperm number of the boars.

The invention belongs to the technical field of preparation of molecular markers for livestock, and particularly relates to a preparation method of a sheep lambing number trait related molecular marker serving as MAS (marker-assisted selection) as well as an application of the molecular marker. The molecular marker is obtained through LH (luteinizing hormone) beta gene cloning, and the nucleotide sequence is represented in SEQ ID NO.1. One C713-T713 base is substituted at 713 bp in the SEQ ID NO.1, so that BSrB I-RFLP (restricted fragment length polymorphisms) is caused. The invention further discloses a primer used in a DNA (deoxyribonucleic acid) sequence of an amplified LH beta gene part and a polymorphism detection method, and provides one novel molecular marker for MAS of the sheep lambing number trait.

The invention belongs to biotechnology field, and relates to a method for developing a dendranthema SSR (Simple Sequence Repeat) primer based on transcriptome sequencing. A lot of sequence information is processed in batch for searching an SSR sequence and designing an SSR labeled primer based on the transcriptome sequencing by utilizing EST-SSR (Expressed Sequence Tags-Simple Sequence Repeat) interspecific metastasis and using a Perl (Practical Extraction and Reporting Language) programming language method in combination, so that the defects of low efficiency, long time consumption, high cost and the like of the SSR development are conquered. Different dendranthema materials are selected for verifying the designed SSR primer, and the primer is a successful SSR primer if detected out by any strip. By adopting the method, 1788 pairs of SSR primers are successively designed, so that a novel method and thinking are provided for the development of the dendranthema SSR primer to further achieve molecular marker assistant selection breeding and comparative genomics research.

The present invention belongs to the technical field of screening and application of pig genetic markers, and in particular relates to a genetic marker related to porcine semen quality traits and application, the genetic marker is a gene obtained by cloning of a pig RNF4 gene 3 'untranslated region, the single nucleotide polymorphism (SNP) can be used as a genetic marker of the porcine semen quality traits, a nucleotide sequence of the genetic marker is as shown in SEQ ID NO: 3 or SEQ ID NO: 4, the sequence SEQ ID NO: 3 or SEQ ID NO: 4 has an allelic gene mutation at 487bp, and AluI-RFLP polymorphism is caused by the allelic gene mutation. Screening step of the method are as follows: extraction of genomic DNA from pig blood, primer design, PCR amplification, PCR product cloning, sequencing, sequence comparing analysis, single nucleotide polymorphism detection, marking and semen quality trait correlation analysis. The genetic marker can be used in pig marker assisted selection.

The invention discloses a breeding method for a corn male sterile line. A male sterile line of corn selfing line is obtained by backcross breeding with corn S-type cytoplasmic male sterile line MD32CGMCC No.8657 as a donor and the corn selfing line as an acceptor. Experiments demonstrate that the breeding method provided by the invention has the following advantages that (1) by combining a marker-assisted selection technology and the backcross breeding technology, the breeding speed of the sterile line is accelerated and Jing 724 sterile line S-jing 724 can be obtained only by three backcross generations; and (2) the S-type sterile line MD32 selected by the breeding method presents stable and thorough sterility in a plurality of genetic backgrounds and is not used in reported studies on corn hybrid male sterile breeding.

The invention relates to a multi-gene high rice blast resistance material breeding method adopting marker assisted selection, and belongs to the field of rice breeding. According to the method, specific molecular marker assisted selection of rice blast resistance genes pita and pi5 is adopted, field resistance identification is combined, and accordingly, a new disease-resistant variety can be bred effectively. The method comprises implementation modes as follows: 1, parents P1 and P2 containing the rice blast resistance genes pita and pi5 respectively are hybridized with a susceptible parent Liaoxing 1 to establish a resistant and susceptible offspring group; 2, extraction of DNA of single resistant and susceptible offspring, PCR (polymerase chain reaction) amplification and electrophoretic analysis of different markers are performed; 3, the parents P1 and P2, the susceptible variety Liaoxing 1 and the single plant of the hybridized offspring group are planted in a rice blast high incidence area Donggang, Dandong, and leaf blast and spike and stem blast resistance identification is performed at a seedling stage and a maturation stage respectively. In combination of the resistance gene marker selection, the new multi-gene rice blast resistance variety containing pita and pi5 is directionally improved and cultivated.

The invention discloses a molecular marker relevant to chicken growth traits, as well as an identification method and application thereof. The molecular marker is C / T base mutation at the 994th base in the sequence shown as SEQ ID NO:1. The molecular marker takes a DNA sequence of chicken BMP7 gene as a template, and selects primers shown as SEQ ID NO:2 and SEQ ID NO:3 for amplification. Through the detection of the C / T mutation site, the genotype of relevant growth traits of a target chicken flock can be known, the chicken flock can be selected on purpose, and the consistencies of weight, shin length and shin girth of a descendant chicken flock can be improved. In the breeding process, TT genotype individuals are selected, and chicken growth indexes such as weight, shin length, shin girth and the like can be improved. Moreover, the identification method used in the invention is high in accuracy, low in cost and high in efficiency.

The invention provides a rice blast resistance gene Pi1 functional specificity molecular marker and application thereof. The molecular marker in a specificity banding pattern with a rice blast resistance gene Pi1 is amplified from a rice genome DNA through primer pairs of SEQ ID NO.1 and SEQ ID NO.2. The rice blast resistance gene Pi1 functional specificity molecular marker has very important application value, and by means of the marker, the utilization efficiency of the gene in germplasm resource screening, marker-assisted selection breeding, gene pyramiding breeding and transgenosis breeding can be improved.

The invention provides a lysinecorn SSR (simple sequence repeat) molecular marker auxiliary selecting and breeding method. By combining a backcross transforming method with a molecular marking method, the method successfully constructs the lysinecorn isogenic gene systems QZ58, Q478 and QC72. After the SSR molecular marker auxiliary selecting and breeding method is used the mono-clones which contain the O2 gene can be detected by SSR molecular marker in the O2 gene after one generation is backcrossed, and the second generation can be backcrossed without selfing, so that the breeding is short in period, low in cost, and high in efficiency. Aiming at different breeding target requirements, the method is used for performing the gene type detection to the backcross offspring seedling leaves; and the method is not influenced by the environment conditions and combines with south propagating and generation adding, so that the target genes are transferred into a good material within a shorter time.

The invention discloses a method for selecting and breeding a green-shin dominant white-feather broiler chicken strain and belongs to the technical field of molecular breeding. The marker-assisted selection method is adopted for identifying the genotype of the locus of dominant white feathers of chicken, the genotype of the locus can be quickly figured out, and the defects that test cross is complex, generation intervals are long and the like in conventional breeding are overcome. According to the green-shin dominant white-feather broiler chicken strain with the genotype II is produced by adopting the method, the complexity of test cross selecting and breeding is avoided, the generation intervals are shortened, the process of breeding is accelerated, and breeding cost is lowered. Moreover, the meat quality of the chicken strain is fine and tender, the taste of the meat is delicious, production performance is high, carcasses of the chicken do not have colored feather vestiges, and therefore the carcasses look better. Besides, the strain is used as a male parent to hybridize and match a high-yield laying hen breed or a fast-growth broiler chicken breed, and high-yield green-shin dominant white-feather broiler chicken or fast-growth green-shin dominant white-feather broiler chicken can be produced.

The invention discloses a molecule labeling method for maker-assisted selection of pig backfat thickness. Through determination of a nucleotide at the 650th locus of a 5' end of a sequence of SEQ ID NO: 1 and a nucleotide at the 95th locus of a 5' end of a sequence of SEQ ID NO: 2, genotypes of the 650th and the 95th loci of a pig are determined so that the backfat thickness of the same type or variety of pigs can be determined. The molecule labeling method for maker-assisted selection of pig backfat thickness can be used for detecting thickness of backfat between the 6th and the 7th ribs of a living pig, thickness of backfat located at the last rib of a living pig, average thickness of backfat of a living pig, and average thickness of backfat of a pig carcass thereby showing the shape and the thickness of pig backfat and thus through maker-assisted selection, pig backfat thickness is reduced; a pig lean meat ratio is improved; and novel important molecular markers are provided. The molecule labeling method for maker-assisted selection of pig backfat thickness plays an important role in pig breeding.

The invention relates to a molecular marker for assisted selective breeding of upland cotton with greensickness resistant traits, belonging to the technical field of genetic breeding of cotton. Three SSR (Simple Sequence Repeat) markers for assisted selective breeding of the upland cotton resisting greensickness can be obtained by constructing a molecular marker genetic linkage map in upland cotton seeds to carry out QTL (Quantitative Trait Locus) positioning on the greensickness resistant traits and selecting remarkable SSR markers entering a linkage group section and the SSR makers linked with disease-resistant QTLs to screen different upland cotton plant systems. The three markers comprise marker primers and a primer combination and are suitable for greensickness-resistant molecular marker-assisted selective breeding of the upland cotton. The plant systems resisting the greensickness can be obtained by using the obtained molecular marker to carry out greensickness-resistant molecular marker-assisted selection on different upland cotton plant systems.

A Solanum lycopersicum plant including within its genome at least one Tomato Spotted Wilt Virus (TSWV) resistance allele and at least one Phytophthora infestans resistance allele. The resistance alleles are present in the coupling phase at different loci on one chromosome and the plant is resistant against TSWV and resistant against at least Phytophthora infestans. A method for producing a hybrid Solanum lycopersicum plant including (a) obtaining a Solanum lycopersicum plant having within its genome at least one Tomato Spotted Wilt Virus (TSWV) resistance allele and at least one Phytophthora infestans resistance allele in the coupling phase; and (b) crossing the Solanum lycopersicum plant with a second Solanum lycopersicum plant bearing an additional resistance allele.