313 results about "Marker-assisted selection" patented technology

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention relates to a pigeon whole genome 5K liquid phase chip and a manufacturing method and application thereof, and belongs to the technical field of molecular biology. According to the pigeon whole genome 5K liquid phase chip disclosed by the invention, the pigeon whole genome 5K liquid phase chip is composed of 5483 SNP molecular markers, and the positions of the SNP molecular markers on a pigeon reference genome Cliv1.0 are as shown in a table 1. Whole genome re-sequencing is carried out on local pigeon varieties and introduced pigeon varieties, quality control screening is carried out through the SNP locus screening strategy to obtain a core SNP locus set, functional SNP loci related to growth and reproductive performance are added, and the method has the advantages of being wide in genetic range, high in pertinence, low in detection cost and high in detection efficiency and has good application prospects. The method has the beneficial effects that the method has important significance on accurate identification and evaluation of pigeon germplasm resources and molecular marker-assisted selective breeding.

The invention belongs to the technical field of molecular biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker combination for single apple remarking assisted selection, a probe group and application of the SNP marker combination and the probe group. The SNP marker combination is composed of 16 SNP markers; the 16 SNP markers comprise SNP1-SNP16, and the number of the SNP markers is 16; the nucleotide sequences of the SNP1-SNP16 are sequentially as shown in SEQ ID NO. 1 to SEQ ID NO. 16. The invention focuses on controlling major variation sites of apple plant single fruit weight, develops 16 major SNP markers linked with apple single fruit weight traits, and is suitable for marker-assisted selection of apple single fruit weight traits in the seedling stage of breeding materials.

The invention discloses a KASP molecular marker for identifying wheat precipitation values in multiple environments and application, and relates to the technical field of biology, in particular to a method for screening or assisting in screening wheat with different precipitation values, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qSV6A.1a or genotype qSV6A.1b, and detecting the precipitation value gt of the wheat with the genotype qSV6A.1b; the precipitation value of wheat of the genotype qSV6A.1a; the wheat of which the genotype is qSV6A.1b is wheat of which the genotype is GG homozygous based on a C101G SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qSV6A.1a is the wheat of which the genotype is CC homozygous based on a C101G SNP (Single Nucleotide Polymorphism) site; the C101G SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO.4 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high precipitation value.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a method for identifying duck palm weight and related molecular marker application thereof, and relates to the technical field of molecular marker-assisted selection, a primer pair is used for amplifying a DNA fragment containing a 63823029th base polymorphic site from the 5'terminal on a fourth chromosome of a duck reference genome IASAASPekinDuckT2T, and the primer pair is used for amplifying a DNA fragment containing the 63823029th base polymorphic site from the 5 '-terminal on the fourth chromosome of the duck reference genome IASAASPekinDuckT2T. The duck reference genome IAASAASPekinDuckT2T is a duck reference genome sequence in a GenBank database, and the duck reference genome sequence is a duck reference genome sequence in the GenBank database; the duck is a Chinese and new white feather meat duck, and the primer pair consists of a DNA (deoxyribonucleic acid) molecule as shown in SEQ ID No.2 and a DNA molecule as shown in SEQ ID No.3. According to the method for identifying the duck foot weight and the application of the related molecular marker, the genotype of the duck can be judged by detecting the genome DNA of the duck, early living screening of the duck foot weight character is achieved, phenotype determination after slaughtering is not needed, the breeding period is remarkably shortened, and the breeding cost is reduced.

The invention discloses a molecular marker for identifying or assisting in identifying soybean oil content and application, and relates to the technical field of biology and application of an SNP (Single Nucleotide Polymorphism) locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 41859817th SNP on a soybean chromosome 5, and the SNP is the 41859817th SNP on the soybean chromosome 5. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is TT homozygosis, the corresponding genotype is A; when the site is GG homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention belongs to the technical field of molecular biology, and particularly discloses a cotton 10K functional site breeding chip and application thereof. The breeding chip is named as' Cotton Core No.1 ', and comprises a 10K functional site targeting capture probe group designed based on a reference genome of disease-resistant, high-quality and high-yield modern upland cotton No.8, the probe group comprises 11159 SNP (Single Nucleotide Polymorphism) loci, 3981 of the SNP loci are functional loci associated with 13 important agronomic characters, and 7178 of the SNP loci are background loci reflecting genetic diversity; the SNP site information is shown in the specification table 1. The invention discloses a cotton 10K functional site breeding chip and application thereof, the breeding chip contains functional sites with more index function genetic variation, has higher detection accuracy among different cotton varieties, is applied to cotton molecular marker-assisted selection breeding, whole genome selection breeding and whole genome correlation analysis, and has higher detection accuracy. The breeding efficiency can be obviously improved, and the practical application is wider.

The invention relates to an InDel molecular marker related to female characters of bitter gourds and application of the InDel molecular marker. According to the invention, an InDel molecular marker related to the strong female character of the bitter gourd is developed, and an amplification primer of the molecular marker related to the strong female character of the bitter gourd is provided, so that the strong female bitter gourd can be quickly and accurately screened in a bitter gourd seed or seedling stage, the auxiliary selection of the molecular marker for female breeding of the bitter gourd is realized, and the breeding efficiency of a female line of the bitter gourd is improved; the method plays an important role in bitter gourd breeding.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention belongs to the field of biotechnology and livestock breeding, and relates to an SNP (Single Nucleotide Polymorphism) molecular marker closely related to sheep weight traits, a primer group, a kit and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and Ggt exists at the 46th bp position of the sequence as shown in SEQ ID NO.4; c mutation. According to the method, the sheep population with excellent genetic resources can be rapidly established by rapidly and accurately detecting the SNP marker related to the weight character of the sheep genetic resource population of the'Auodur Lake ', so that the marker-assisted selection breeding process of the weight character of the sheep is accelerated.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of male semi-fine wool sheep and application of the SNP molecular marker. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of elephant male semi-fine wool sheep. The SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of a Lanblet sheep reference genome ARS-UIRambv3.0, GCF016772045.2, and the SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of the Lanblet sheep reference genome ARS-UIRambv3.0; the mutant basic group is G or T. By detecting the basic group of the 24648039th nucleotide site on the fifth chromosome of the elephant male semi-fine wool sheep, the content of immune globulins IgA, IgG and IgM of an elephant male semi-fine wool sheep individual can be judged, and a new SNP molecular marker resource is provided for non-diagnostic elephant male semi-fine wool sheep immune character marker-assisted selection.

The invention discloses an application method of using an ERC1 gene as a cattle superovulation character molecular marker, and belongs to the technical field of animal character detection gene engineering. The length of the nucleotide sequence of the ERC1 gene segment is 520bp, and a c-t base mutation exists at the 293bp position. The mutation site is used as a molecular marker, the genotype is judged through sequence determination, the genotype can be associated with the excellent ovulation character of the cattle, and the ovulation performance of individuals with different genotypes is remarkably different. According to the invention, a specific primer is utilized to obtain an ERC1 gene part segment related to the excellent superovulation character of cattle, and a specific SNP site in the segment is taken as a molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention belongs to the field of biotechnology and livestock breeding, and relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep meat quality traits, a primer group, a kit and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and Ggt exists at the 46th bp position of the sequence as shown in SEQ ID NO.4; a is a mutation site. By rapidly and accurately detecting the SNP marker related to the meat quality traits of the'Auodurou Lake 'sheep genetic resource population, the'Auodurou Lake' sheep variety dominant genetic resource population can be rapidly established, so that the marker-assisted selection breeding process of the sheep meat quality traits is accelerated.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker synergistically related to growth and reproduction traits of chickens and application, and belongs to the technical field of molecular biology. The SNP molecular marker related to reproduction is located at the 28973618th nucleotide of chromosome 6 of a reference sequence of a GRCg6a version of a chicken genome, and has C / G polymorphism. The growth-related SNP molecular marker disclosed by the invention is positioned at the 2897383838th nucleotide of chromosome 6 of a reference sequence of a GRCg6a version of a chicken genome, and has G / A polymorphism. The sperm density and the keel length of a diplotype CCAA individual are the highest, and the growth traits and the reproduction traits are in a synergistic relationship. The SNP molecular marker disclosed by the invention can be used for carrying out marker-assisted selection on characters such as chicken sperm density and keel length, implementing early selection and improving the breeding efficiency. The invention provides a reliable molecular marker for the genetic improvement of chicken growth and reproduction traits, and has important significance for the genetic improvement of chicken.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located at the 6293801th basic group on the No.15 chromosome of a goat, the specific SNP marker site is C / G basic group mutation at the 392bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of a CC genotype goat individual and a CG genotype goat individual at the site is remarkably higher than that of a GG genotype individual. The method comprises the following steps: by taking a Muncheng black goat as a research object, carrying out PCR (Polymerase Chain Reaction) amplification on a segment of ENSCHIG00000009743 gene, wherein the segment contains a goat 15 # chromosome g.6293801Cgt; according to the present invention, the DNA sequence of the G mutation site is analyzed, the influence of the specific genotype of the site on the lambing number of the Mancheng black goats is analyzed, and the individual breeding of the Mancheng black goats is performed according to the influence, such that the Mancheng black goat lambing number can be increased, and the breeding of the new variety (strain) using the Mancheng black goats as the breeding material can be accelerated; and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The application discloses a G101T SNP related to wheat wet gluten content, which is located at the physical position 80813538 bp of the 6A chromosome in the Chinese spring wheat genome IWGSC RefSeq v1.0, and the genotype is G or T. The application develops a KASP marker and a KASP primer group for identifying the wheat wet gluten content based on the SNP site, and provides a method for identifying or assisting in identifying the wheat wet gluten content, and it is found through the method that the wheat wet gluten content of the G101T SNP site genotype TT is higher than or higher than the wheat wet gluten content of the genotype GG. The application has important theoretical significance and economic value for utilizing molecular marker assisted selection to obtain wheat germplasm or breeding offspring materials with higher wet gluten content.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant comprising a quantitative trait locus (QTL) or a causal polymorphism associated with a flower density trait, and to Cannabis spp. plants having a flower density trait of interest comprising defined allelic states of polymorphisms defining the QTL or defined allelic states of causal polymorphisms provided herein. Also provided are Cannabis spp. plants with a flower density trait of interest comprising defined allelic states of polymorphisms and plants identified, characterized or produced by the methods described. Further provided are methods of marker assisted selection, genomic selection, marker assisted breeding, and genetic modification, for obtaining plants having a flower density trait of interest.

The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and / or Ant2 gene allowing color sorting of seeds.

The present disclosure provides methods and compositions for producing, detecting, and selecting soybean plants and seeds comprising at least one high protein CCT (CONSTANS, CO-like and TOC1) domain containing variant allele and introgressing the high protein CCT variant allele into soybean plants. The present disclosure also provides methods and compositions for producing, detecting, and selecting soybean plants producing seeds having a high protein content including breeding methods for introgressing high protein alleles into soybean plants using marker assisted selection using markers linked to or associated with high protein CCT in soybean.

The invention discloses a dCAPS molecular marker for detecting the cold demand of peaches and application of the dCAPS molecular marker, and belongs to the technical field of fruit tree molecular marker-assisted selective breeding. The nucleotide sequences of primers for amplifying the molecular marker are as shown in SEQ ID NO.1 and SEQ ID NO.2. The invention also discloses a kit for detecting the molecular marker. A dCAPS molecular marker method for detecting the cold demand of peaches comprises the following steps: (1) extracting genomic DNA of peach leaves; (2) carrying out PCR (Polymerase Chain Reaction) amplification through the primer; and (3) after the PCR product is subjected to BspHI enzyme digestion, detecting through gel electrophoresis: if the enzyme digestion product only contains a 194bp band, determining that the detected peach is a low-cold-demand variety (CR < = 600h), and if the enzyme digestion product does not contain a 194bp band, determining that the detected peach is a low-cold-demand variety (CR < = 600h); and if the product only contains a band of 156bp, determining that the detected peach is a high-cold-demand variety (CR is greater than or equal to 800h). The invention provides a molecular marker for rapidly identifying the cold demand at the peach seedling stage, and overcomes the defects of long observation period and large environmental interference of the traditional phenotype; (2) the breeding period can be remarkably shortened through molecular marker-assisted selection, the screening efficiency of varieties with different cold requirements is improved, and the breeding requirements of varieties in different ecological regions are met.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

The invention provides a molecular marker used for Tibetan sheep marker-assisted selection and related to disease resistance characters and application of the molecular marker, and belongs to the technical field of molecular marker-assisted breeding, the molecular marker is located at the 29402282th basic group on the 10th chromosome of the international sheep reference genome Oarv4.0 version; the variation type is T / C, the molecular marker site has three genotypes of TT, TC and CC, the genotype of the molecular marker site has significant correlation with the content of cytokines in a Tibetan sheep blood sample, and the content of IL-1alpha, IL-1beta, IL-6, TNF-alpha, IL-1Ra and IL-10 of a Tibetan sheep individual can be judged by detecting the genotype of the SNP molecular marker. The SNP molecular marker can be used for non-diagnostic Tibetan sheep immune trait marker-assisted selection, and a new SNP molecular marker resource is provided for early molecular breeding of Tibetan sheep.