4results about How to "Good character" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The present application belongs to the technical field of plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials, cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct organogenesis route. The present application realizes the cyclic production of Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic germination method, and the problems of large offspring trait separation and different qualities caused by the seed asymbiotic germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new technical support for the factory-scale growth of Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and survival rate of seedlings are high.

The application discloses a preparation method and production process of a six-membered aromatic ring fused pyrrole compound, and belongs to the field of organic synthesis. The six-membered aromatic ring fused pyrrole compound of the target compound can be obtained in a high yield even under normal pressure by using specific low-boiling-point solvents (a mixed system of toluene and water). In addition, in the post-treatment process after the reaction is completed, a method of sequentially using sodium chloride aqueous solution washing and t-butyl methyl ether beating is used, so that the by-products can be effectively removed in the post-treatment process, and the product properties are improved.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.