71results about How to "Good character" patented technology

The invention discloses an Indel molecular marker for identifying ricefield eel strains in two Guangdong regions. The nucleotide sequence of the Indel molecular marker A is shown as SEQ ID NO: 5 or SEQ ID NO: 7; the nucleotide sequence of the Indel marker B is as shown in SEQ ID NO: 6 or SEQ ID NO: 8. The invention also provides a primer pair for amplifying the Indel molecular marker. By adopting the Indel molecular marker primer pair provided by the invention to amplify genome DNA of ricefield eel strains in different regions, the ricefield eel strains in the two regions can be quickly screened out according to amplified fragments. The Indel marker provided by the invention can be applied to rapid identification of germplasm resources of a specific geographical strain so as to obtain excellent characters of the strain and finally realize precise breeding.

The invention relates to a growth control method, system and device for sugarcane hybrid flower panicles and a medium, and the method comprises the steps: obtaining a plant image of a sugarcane parent in a planting area, and carrying out the image recognition processing of the plant image, and obtaining the parent flowering phase type of the sugarcane parent; determining the current growth condition of the sugarcane parent in the current growth period according to the plant image; obtaining seed characteristics of the sugarcane parents and environmental factors in the planting area, and determining a target induction scheme corresponding to the sugarcane parents according to the flowering phase type, the current growth condition, the environmental factors and the seed characteristics of the parents; and adjusting the environmental factors of the sugarcane parents according to the target induction scheme. By means of the method, intelligent management and control can be conducted on the spikes of the sugarcane parents, the characters of the sugarcane parents can be purposefully improved on the basis of the management and control process, and compared with a manual management and control mode, the method is faster, more effective and more suitable for large-scale sugarcane planting scenes.

The invention relates to the technical field of soil improvement, and discloses a multiple-effect soil conditioner based on waste and inferior tobacco leaves and a preparation method and application of the multiple-effect soil conditioner based on the waste and inferior tobacco leaves. 24-28% of mushroom residue; 24-28% of tea seed shells; 10-15% of pig manure; the preparation method comprises the following steps: collecting raw materials including waste tobacco leaves, mushroom residues, tea seed shells and pig manure, detecting and proportioning the raw materials, adding the bacillus subtilis into the raw materials, uniformly mixing, fermenting for thirty days, turning once every ten days during fermentation, and detecting again to obtain the multi-effect soil conditioner. The soil conditioner is applied to the ploughed farmland according to 100 kg / mu to 150 kg / mu and covered with soil, then the farmland covered with the soil is ploughed by 8 cm to 12 cm, flue-cured tobaccos are planted, and the conditioner can achieve the effects of improving the physical and chemical properties of soil, promoting the growth and development of the tobaccos, improving the yield and quality of the tobacco leaves and enhancing the stress resistance of the tobaccos.

The present invention discloses a chitinase-producing desert bacillus and its application, and relates to the field of microbial technology. The present invention discovers a new microbial strain, which is identified as belonging to the genus Desert Bacillus and is named Desert Bacillus sp.MYHB_202305ChI. Experimental verification shows that the strain has the ability to tolerate high salt and secrete chitinase.

The invention discloses an external preparation of a microsphere-loaded medicine for treating chloasma and a preparation method of the external preparation. The preparation method comprises the following steps: preparing polyvinyl alcohol acrylate microspheres, loading a tranexamic acid medicine and adding a matrix to prepare the external preparation of the microsphere-loaded medicine. By means of oleophylic and hydrophilic segments on the polyvinyl alcohol acrylate microspheres, fusion with skin lipid components is increased, permeability of the medicine is improved, meanwhile, due to the large specific surface area of the microspheres, the medicine can be more evenly loaded on the microspheres, and due to the hydrophilicity of the microspheres, moisture can be kept for a longer time; and the problem of crystallization of tranexamic acid caused by moisture volatilization is well solved. The ointment or gel external preparation prepared by the invention is relatively good in character and stability, good in in-vitro permeation and in-vitro release performance and remarkable in treatment effect on chloasma of mice.

The invention belongs to the technical field of fish genetic breeding, and particularly relates to a creation method of an anti-herpes virus double-triploid carp and crucian carp cloning line with a growth advantage. The method comprises the following steps: breeding a gynogenesis double-triploid prussian carp female parent and a sexual reproduction double-diploid prussian carp male parent to obtain a filial generation G1, screening male fish from the filial generation G1, and hybridizing the male fish with double-diploid Yellow River carp female fish to obtain a double-triploid prussian carp filial generation G2; female fish in the filial generation G2 is used as a female parent, natural gynogenesis is carried out on the female parent and the male parent Xinguo red carp, a filial generation G3 is obtained, and the G3 is an anti-herpes virus double-triploid carp and crucian carp cloning line. A brand-new breeding route for creating the bitriploid carp and crucian carp cloning line is established, genomes of Yellow River carps, prussian carps and crucian carps are integrated and reconstructed, so that the bitriploid carp and crucian carps form obvious heterosis in blister disease virus resistance and growth speed, and the bitriploid carp and crucian carp cloning line has a wide application prospect.

The invention discloses a method for inducing apocarya stems to generate transgenic hairy roots, and belongs to the field of forest tree genetic engineering. According to the method, the semi-lignified or lignified stems of the apocarya are infected by utilizing the agrobacterium rhizogenes to obtain stable transgenic hairy roots, rooting can be induced within 1-2 months, the rooting rate is as high as 94.43%, and the transgenic positive rate can reach 98.38%. The invention provides an economical and efficient non-tissue culture dependent transgenic method, it is clear that transgenic roots can be used for gene function verification, the method breaks through the technical obstacle that stable genetic transformation of the carya illinoensis is difficult, and technical support is provided for research of functional genes.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The present invention belongs to the field of plant genetic engineering. Specifically, the present invention provides a nucleic acid construct. The present invention adopts a nucleic acid construct with a specific structure to improve plant traits and enhance plant heat resistance, and has broad application prospects.

The invention discloses a granular bio-fertilizer and a preparation method thereof, and the granular bio-fertilizer comprises the following components in percentage by weight: 94-97% of a base fertilizer, 2-5% of a synergist and 1-2% of an auxiliary agent. The granular bio-fertilizer is a fertilizer prepared by compatibility of one of an inorganic fertilizer, an organic fertilizer and an organic and inorganic fertilizer with a bio-fertilizer synergist. The granular bio-fertilizer disclosed by the invention can adopt an inorganic fertilizer, an organic fertilizer or an organic and inorganic fertilizer as a base fertilizer, can be suitable for almost all granular fertilizers (except for fertilizers with a killing function on microorganisms or an antagonistic effect on components in a bio-fertilizer synergist) in the current market, and is simple in production process, relatively low in cost and suitable for industrial production. Moreover, the added value of the product can be effectively improved, and the production process belongs to a novel fertilizer production process and has a huge market popularization value.

The invention relates to the technical field of organic fertilizers, in particular to a compound organic fertilizer and a preparation method thereof. Comprising the following raw materials in parts by weight: 100-600 parts of an organic carbon fertilizer, 40-80 parts of a nitrogen fertilizer, 80-300 parts of a potassium fertilizer and 3-10 parts of a chelated trace element fertilizer. The organic leavening formed by synergistically combining chicken manure, cow dung and pig manure is used as a fertilizer raw material, so that waste is turned into wealth, and the preparation cost of the fertilizer is reduced. The multi-element chelated trace element fertilizer has a remarkable plant growth regulation effect, can effectively promote yield and income increase, and greatly improves the nutritional degree required by plants, so that the growth of crops can be greatly promoted; medium elements generated in the waste after microbial fermentation contain aromatic hydroxy carboxylic acid, carbohydrate, amino acid and protein, and have significant effects on improving soil properties, promoting crop growth and improving crop quality.

The application discloses a nitrogen-containing compound, cross-linked hyaluronic acid and application thereof. Specifically, the application discloses a compound as shown in formula I or a salt thereof. The cross-linked hyaluronic acid prepared by cross-linking hyaluronic acid with the nitrogen-containing compound has good properties and high enzymatic stability, preferably, the enzymatic stability is better than that of a commercial cross-linked hyaluronic acid Durolane.

This invention provides a turbot spleen tissue cell line, with the accession number CCTCCNO:C2022229. The turbot spleen tissue cell line provided by this invention can be continuously passaged, and experimental results show that it remains stable after 65 passages, providing a large number of stable turbot spleen cells for research on immune function genes, etc. The provided cell line has excellent characteristics; it consists of fibroblasts, with spindle-shaped or irregularly triangular cells, several irregular protrusions extending outward from the cell body, and the cells are closely arranged. It can be passaged every 3-4 days, with high recovery efficiency, and can be used for plasmid transfection, siRNA interference, and various other molecular biology experiments.

The invention discloses an engineering bacterium producing ergothioneine and a construction method and application thereof. The engineering bacterium construction method is to use Escherichia coli as a starting strain, and to introduce a constitutive promoter PrrnD and a new gold Mycobacterium Mycobacterium neoaurum Ergothioneine biosynthesis gene cluster egtABCDE Integrate into the genome of the starting strain; further convert the ATP of the resulting strain into phosphoribosyl kinase HisG The 271st glutamic acid of gene is mutated into lysine, and the engineering bacteria of producing thioneine is obtained. With glucose as main raw material, the engineering bacteria of the present invention are utilized to carry out fermentation culture, and thioneine can be produced. The engineering bacteria fermentation synthesis of thioneine of the present invention has the advantages of simple process and high production efficiency, can avoid the use of a large amount of organic solvents and antibiotics, and is suitable for industrialized production.

The utility model discloses a PCIE (Peripheral Component Interface Express) adapter plate fixing structure, and relates to the technical field of computer hardware. According to the PCIE adapter plate fixing structure, the PCIE adapter plate is buckled through the PCIE adapter plate side edge pressing and touching adhesive tape structure along the end binding and fixing belt, so that the PCIE adapter plate is very stable and is prevented from slipping off, during fixing, the case inner wall pressing and buckling piece is bonded with the inner wall of the nearby case through the double-sided adhesive film, fixing can be achieved, and during detaching, tearing and detaching can be conducted by lifting the piece holding structure, so that the fixing structure is convenient to use. Therefore, the effect of conveniently adapting to fixing application of plates with different length specifications is achieved, the edge of the clamping body is wrapped in a strip-type design, the occupied space in a machine box is greatly reduced, the character of the space in the machine box is optimized, and the effect of optimizing the space is achieved; and the specification information of the card body can be conveniently filled on the label card paper and then inserted into the label corner frame mechanism, so that the information of the card body can be labeled, the character of conveniently labeling the information of the card board is achieved, and the effect of improving the management efficiency is achieved.

The present application belongs to the technical field of plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials, cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct organogenesis route. The present application realizes the cyclic production of Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic germination method, and the problems of large offspring trait separation and different qualities caused by the seed asymbiotic germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new technical support for the factory-scale growth of Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and survival rate of seedlings are high.

The cyclic preparation method of the sodium antimonate provided by the embodiment of the invention comprises the following steps: adding antimony trioxide and potassium hydroxide into deionized water, stirring, mixing and dissolving, heating and slurrying, and then adding hydrogen peroxide to carry out oxidation reaction to obtain a first potassium antimonate solution; adding sodium hydroxide and a surfactant into the first potassium antimonate solution to carry out replacement reaction, standing, cooling, washing and drying to obtain a sodium antimonate finished product; potassium hydroxide is added into the filtered reaction liquid after standing, a second potassium antimonate solution is obtained, and the second potassium antimonate solution is used for circularly preparing the sodium antimonate finished product. On one hand, the product obtained in each cycle is good in character, the antimony content and the arsenic content basically meet the requirements, and the yield is considerable; on the other hand, the cost can be controlled, and the utilization rate of alkali liquor in the sodium antimonate preparation process is increased.

The invention belongs to the technical field of tissue culture, and relates to a pithecellobium clypearia filament callus induction method and application, and the method comprises the following steps: taking pithecellobium clypearia buds, disinfecting and sterilizing with a disinfectant, taking pithecellobium clypearia filaments as explants in a sterile environment, inoculating the explants into an induction culture medium for dark culture to form calluses, and carrying out tissue culture to obtain the pithecellobium clypearia filament callus. And then transferring the callus to a proliferation culture medium for subculture proliferation to obtain the callus with higher quality. The induction method provided by the invention can induce pithecellobium clypearia filaments to quickly form calluses, and the induction rate of the calluses is as high as 100%; the induction method of the pithecellobium clypearia filament callus is short in cultivation period, high in induction efficiency and low in pollution rate, the research path of a pithecellobium clypearia rapid propagation technology is widened when the induction method is applied to pithecellobium clypearia cultivation, it is possible that pithecellobium clypearia filaments are regenerated into plants, and high-quality plants with excellent characters can be cultivated.

The present disclosure provides a method for producing biological organic fertilizer under forests. The method includes: culturing the strain of white rot fungus WH‑B‑X3 in the first straw to obtain a first-level solid strain; inoculating the first-level solid strain on the second straw to obtain a second-level solid strain; adding the second-level solid strain to the second liquid strain culture medium in a large fermentation tank to obtain a third-level liquid strain; inoculating the third-level liquid strain on the third straw, and laying the inoculated third straw on the soil under the forest for cultivation to obtain biological organic fertilizer. When the white rot fungus WH‑B‑X3 is used to produce biological organic fertilizer, the straw can be locally sourced, the straw is directly crushed and sprayed in the field, the third straw is obtained, and the inoculation is placed under the forest for aerobic fermentation, and the preparation of biological organic fertilizer can be realized, and the production process is simple, the cost is low, and it is conducive to wide promotion and application.

The invention discloses a preparation method of a decitabine impurity III, and belongs to the field of chemical pharmacy. The decitabine impurity III belongs to a degradable impurity and can be subjected to material conversion in synthesis, transportation and storage processes, and the decitabine impurity III obtained by alkali degradation, separation and purification, desalination and freeze-drying has the advantages of high yield, high purity and simple preparation method. A reliable impurity reference substance can be provided for quality research of decitabine, so that the product quality is ensured, and the medication safety of decitabine is improved.

This invention relates to the field of plant tissue culture, specifically disclosing a method for rapidly propagating dandelion through tissue culture. The method includes the following steps: inducing callus tissue using dandelion leaves as explants; inducing shoot formation from the callus tissue; and rooting the shoots to obtain dandelion tissue culture seedlings. This invention provides a high-quality and efficient tissue culture propagation method for dandelion by utilizing dandelion leaves as explants, inducing callus tissue through dedifferentiation, allowing the callus tissue to redifferentiate and produce shoots, and then rooting individual shoots.

The present invention discloses a new amylase-producing Zobellella species and its application, and relates to the field of microbial technology. The present invention discovers a new microbial species, which is identified as belonging to the genus Zobellella and is named Zobellella sp. MYHB_202302AmY. Experimental verification shows that the bacteria has the ability to tolerate high salt and secrete amylase.

The invention provides a breeding method of crassostrea gigas' Haida No.3 'fast-growing triploid, and belongs to the technical field of aquaculture genetic breeding. According to the method, a new variety of diploid crassostrea gigas' Haida No.3 'is taken as a basic population, crassostrea gigas' Haida No.3 'fast-growing diploid is generated through breeding, crassostrea gigas' Haida No.3 'triploid and tetraploid are generated through induction, and crassostrea gigas' Haida No.3 'fast-growing tetraploid is generated through breeding. Crassostrea gigas' Haida No.3 'fast-growing tetraploid and diploid are hybridized to generate Crassostrea gigas' Haida No.3 'fast-growing triploid. By evaluating the production performance, the ploidy stability and the genetic diversity of the crassostrea gigas' Haida No.3 'fast-growing triploid, the crassostrea gigas' Haida No.3 'triploid which is fast in growth, pure black in shell color, stable in ploidy and high in genetic diversity is obtained, and the crassostrea gigas' Haida No.3 'fast-growing triploid has important application value in the oyster breeding industry.

The invention discloses an oral soluble film composition containing vardenafil and application of the oral soluble film composition. The oral soluble film composition comprises the following components in percentage by weight: 20.5%-25% of an active substance, 28.3%-32% of microcrystalline cellulose, 2.6%-3.3% of vitamin E, 1.5%-2.1% of a separant, 0.8%-1.1% of a lubricant, 35.6%-41.5% of a film forming material, 1.2%-1.8% of a plasticizer, 0.08%-0.12% of a pH regulator and 2.7%-3.5% of a flavoring agent, the active substance is vardenafil or vardenafil hydrochloride, the concentration of the active substance is 35-45 mg / ml, and the oral soluble film composition is applied to improving the erectile function, prolonging the sexual life time, improving the microenvironment of local spermatogenesis and improving the sperm vitality. The pH regulator is matched, so that the dissolution speed of the medicine can be increased; 40-60 hydroxypropyl methyl cellulose and polyvinyl alcohol are blended to form a film material, so that the film coating effect can be ensured, and the properties of the film can be improved; vitamin E is added in the formula, so that the microenvironment of local spermatogenesis can be improved, the sperm motility is improved, and the medicinal value of the composition is improved.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

The invention provides a method for accelerating directional breeding of bumblebees, which specifically comprises the following steps: selecting worker bees with excellent characters as female parent worker bees for directional breeding, laying eggs and hatching the worker bees into male bees in a dark environment with the temperature of 27-29 DEG C and the humidity of 50%-60%, obtaining sperms of the male bees after sexual maturity, and then putting the sperms into a bumblebee breeding box to breed the bumblebees. And inseminating female parent worker bees by using the obtained sperms, establishing a queen bee breeding group by using the inseminated female parent worker bees and more than 30 worker bees which just emerge and come out of the house to produce offspring queen bees, and mating the offspring queen bees with the male bees to obtain an offspring queen bee colony. According to the method, the queen bee colony can be stably and rapidly obtained only through a single female parent worker bee, all genes come from the female parent worker bee, rapid propagation of bumblebees and breeding of excellent characters are facilitated, and the method has important application prospects in natural resource protection and agricultural production.

The invention provides a blue crab fast-growing strain group breeding method, and aims to improve the growth speed and quality of blue crabs through multi-generation continuous breeding. The method comprises the following steps: firstly, breeding offspring seeds of a basic group, carrying out high-intensity screening in different growth stages, and selecting individuals which grow fastest for breeding; carrying out third screening before reproductive shelling, and selecting male crabs and female crabs which grow fastest; then carrying out free mating and overwintering; the female crabs are screened for the last time in spring, and it is ensured that the selected female crabs are healthy and free of diseases; and finally carrying out egg carrying, membrane rupture incubation and cultivation to form a breeding group. In the breeding process, a far-source population is introduced for germplasm rejuvenation, and a microsatellite (SSR) molecular marker is used for genetic diversity and heritability evaluation. In addition, the method further comprises the steps that specific bait feeding is conducted on the blue crabs in different growth stages, and the breeding environment is strictly controlled. Through the steps, the growth performance and genetic quality of the blue crabs can be effectively improved.

The invention discloses a restoration method for in-situ greening of a red mud dump, comprising the steps of preparing an auxiliary modifier; adding materials for mixing treatment; static maintenance and restoration; modified restoration inspection; and plant test planting. The invention measures the pH value, alkali concentration restoration rate, and the amount of microorganisms and organic matter of a red mud soil mixture. Before actually being put into planting, the restored red mud soil mixture can be put into trial planting by a trial planting method. Based on the measurement of modified restoration parameters, the actual trial planting method is used to further accurately judge the planting effect of the restored red mud soil mixture on plants. By calculating the germination rate of the test-planted plants, it is convenient to judge whether the restored red mud soil mixture can be formally put into plant planting by the germination rate, thereby facilitating the determination of the actual planting effect of the red mud soil mixture on plants by a method of seeing the big from the small.

This invention discloses the application of cotton uORF elements G1u2 and G1u3 in improving gene translation levels, belonging to the field of genetic engineering. This invention utilizes upland cotton... WNK Gene Ghir_D04G003230 Two uORF elements, G1u2 (SEQ ID No. 2) and G1u3 (SEQ ID No. 3), were isolated from cotton. A dual-luciferase reporter assay demonstrated that both G1u2 and G1u3 elements significantly improved the translation efficiency of downstream genes. This invention is the first to discover uORF elements in cotton with a clear positive translation regulatory function, filling a gap in the research and application of positive uORF regulatory elements in cotton. Furthermore, these uORF elements can be applied to molecular design breeding for high-yield, stress-resistant, and high-quality plants, as well as precise gene editing, demonstrating strong practicality.

The invention relates to a hybrid breeding method for a southern group and a northern group of Manila clams, and belongs to the technical field of shellfish genetic breeding in aquaculture. The method comprises the following steps: collecting a northern group and a southern group, taking shell length as a breeding index, selecting the top 10% of individuals in shell length as parents, carrying out fast growth breeding in the southern group and the northern group respectively, obtaining the breeding F1 generation of the southern group and the northern group respectively, and cultivating them to sexual maturity; obtaining the breeding F2 generation of the southern group and the northern group respectively by the same breeding method, selecting well-developed individuals as candidate parents, placing them in the same indoor cultivation pool for synchronous maturation; artificially inducing spawning of parent shellfish, and adopting methods including freshwater flushing, high-concentration algae water feeding and air flow stimulation; and obtaining hybrid offspring showing obvious hybrid advantages in growth and survival.