9results about How to "Good character" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention discloses a granular bio-fertilizer and a preparation method thereof, and the granular bio-fertilizer comprises the following components in percentage by weight: 94-97% of a base fertilizer, 2-5% of a synergist and 1-2% of an auxiliary agent. The granular bio-fertilizer is a fertilizer prepared by compatibility of one of an inorganic fertilizer, an organic fertilizer and an organic and inorganic fertilizer with a bio-fertilizer synergist. The granular bio-fertilizer disclosed by the invention can adopt an inorganic fertilizer, an organic fertilizer or an organic and inorganic fertilizer as a base fertilizer, can be suitable for almost all granular fertilizers (except for fertilizers with a killing function on microorganisms or an antagonistic effect on components in a bio-fertilizer synergist) in the current market, and is simple in production process, relatively low in cost and suitable for industrial production. Moreover, the added value of the product can be effectively improved, and the production process belongs to a novel fertilizer production process and has a huge market popularization value.

This invention provides a turbot spleen tissue cell line, with the accession number CCTCCNO:C2022229. The turbot spleen tissue cell line provided by this invention can be continuously passaged, and experimental results show that it remains stable after 65 passages, providing a large number of stable turbot spleen cells for research on immune function genes, etc. The provided cell line has excellent characteristics; it consists of fibroblasts, with spindle-shaped or irregularly triangular cells, several irregular protrusions extending outward from the cell body, and the cells are closely arranged. It can be passaged every 3-4 days, with high recovery efficiency, and can be used for plasmid transfection, siRNA interference, and various other molecular biology experiments.

The present application belongs to the technical field of plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials, cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct organogenesis route. The present application realizes the cyclic production of Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic germination method, and the problems of large offspring trait separation and different qualities caused by the seed asymbiotic germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new technical support for the factory-scale growth of Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and survival rate of seedlings are high.

This invention relates to the field of plant tissue culture, specifically disclosing a method for rapidly propagating dandelion through tissue culture. The method includes the following steps: inducing callus tissue using dandelion leaves as explants; inducing shoot formation from the callus tissue; and rooting the shoots to obtain dandelion tissue culture seedlings. This invention provides a high-quality and efficient tissue culture propagation method for dandelion by utilizing dandelion leaves as explants, inducing callus tissue through dedifferentiation, allowing the callus tissue to redifferentiate and produce shoots, and then rooting individual shoots.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

The application discloses a preparation method and production process of a six-membered aromatic ring fused pyrrole compound, and belongs to the field of organic synthesis. The six-membered aromatic ring fused pyrrole compound of the target compound can be obtained in a high yield even under normal pressure by using specific low-boiling-point solvents (a mixed system of toluene and water). In addition, in the post-treatment process after the reaction is completed, a method of sequentially using sodium chloride aqueous solution washing and t-butyl methyl ether beating is used, so that the by-products can be effectively removed in the post-treatment process, and the product properties are improved.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention belongs to the field of industrial waste resource utilization technology, and discloses a zero-discharge resource utilization method for polysilicon waste liquid. The method involves adding sodium hydroxide solution to chlorosilane waste liquid and reacting it, then obtaining sodium silicate solution through stepwise extraction with dilute alkali or one-step extraction with concentrated alkali. Furthermore, calcium carbide slag is calcined and slaked to obtain lime slurry. Then, the sodium silicate solution and lime solution are added to a reaction vessel according to a specific ratio and heated for reaction. The resulting mixture is filtered to obtain microporous calcium silicate material. The filtrate can be recycled as an alkaline solution for chlorosilane waste liquid. This invention effectively solves the problems of scarce resource utilization pathways and low utilization efficiency of chlorosilane waste liquid by synergistically utilizing chlorosilane waste liquid and calcium carbide slag solid waste. The resulting microporous calcium silicate material has excellent physicochemical properties. Simultaneously, the process is simple and the conditions are mild, achieving comprehensive utilization of industrial waste liquid and residue while also contributing to energy conservation, emission reduction, and source environmental protection.