26results about How to "Good character" patented technology

The invention discloses an Indel molecular marker for identifying ricefield eel strains in two Guangdong regions. The nucleotide sequence of the Indel molecular marker A is shown as SEQ ID NO: 5 or SEQ ID NO: 7; the nucleotide sequence of the Indel marker B is as shown in SEQ ID NO: 6 or SEQ ID NO: 8. The invention also provides a primer pair for amplifying the Indel molecular marker. By adopting the Indel molecular marker primer pair provided by the invention to amplify genome DNA of ricefield eel strains in different regions, the ricefield eel strains in the two regions can be quickly screened out according to amplified fragments. The Indel marker provided by the invention can be applied to rapid identification of germplasm resources of a specific geographical strain so as to obtain excellent characters of the strain and finally realize precise breeding.

The invention relates to the technical field of soil improvement, and discloses a multiple-effect soil conditioner based on waste and inferior tobacco leaves and a preparation method and application of the multiple-effect soil conditioner based on the waste and inferior tobacco leaves. 24-28% of mushroom residue; 24-28% of tea seed shells; 10-15% of pig manure; the preparation method comprises the following steps: collecting raw materials including waste tobacco leaves, mushroom residues, tea seed shells and pig manure, detecting and proportioning the raw materials, adding the bacillus subtilis into the raw materials, uniformly mixing, fermenting for thirty days, turning once every ten days during fermentation, and detecting again to obtain the multi-effect soil conditioner. The soil conditioner is applied to the ploughed farmland according to 100 kg / mu to 150 kg / mu and covered with soil, then the farmland covered with the soil is ploughed by 8 cm to 12 cm, flue-cured tobaccos are planted, and the conditioner can achieve the effects of improving the physical and chemical properties of soil, promoting the growth and development of the tobaccos, improving the yield and quality of the tobacco leaves and enhancing the stress resistance of the tobaccos.

The invention discloses an external preparation of a microsphere-loaded medicine for treating chloasma and a preparation method of the external preparation. The preparation method comprises the following steps: preparing polyvinyl alcohol acrylate microspheres, loading a tranexamic acid medicine and adding a matrix to prepare the external preparation of the microsphere-loaded medicine. By means of oleophylic and hydrophilic segments on the polyvinyl alcohol acrylate microspheres, fusion with skin lipid components is increased, permeability of the medicine is improved, meanwhile, due to the large specific surface area of the microspheres, the medicine can be more evenly loaded on the microspheres, and due to the hydrophilicity of the microspheres, moisture can be kept for a longer time; and the problem of crystallization of tranexamic acid caused by moisture volatilization is well solved. The ointment or gel external preparation prepared by the invention is relatively good in character and stability, good in in-vitro permeation and in-vitro release performance and remarkable in treatment effect on chloasma of mice.

The invention belongs to the technical field of fish genetic breeding, and particularly relates to a creation method of an anti-herpes virus double-triploid carp and crucian carp cloning line with a growth advantage. The method comprises the following steps: breeding a gynogenesis double-triploid prussian carp female parent and a sexual reproduction double-diploid prussian carp male parent to obtain a filial generation G1, screening male fish from the filial generation G1, and hybridizing the male fish with double-diploid Yellow River carp female fish to obtain a double-triploid prussian carp filial generation G2; female fish in the filial generation G2 is used as a female parent, natural gynogenesis is carried out on the female parent and the male parent Xinguo red carp, a filial generation G3 is obtained, and the G3 is an anti-herpes virus double-triploid carp and crucian carp cloning line. A brand-new breeding route for creating the bitriploid carp and crucian carp cloning line is established, genomes of Yellow River carps, prussian carps and crucian carps are integrated and reconstructed, so that the bitriploid carp and crucian carps form obvious heterosis in blister disease virus resistance and growth speed, and the bitriploid carp and crucian carp cloning line has a wide application prospect.

The invention discloses a method for inducing apocarya stems to generate transgenic hairy roots, and belongs to the field of forest tree genetic engineering. According to the method, the semi-lignified or lignified stems of the apocarya are infected by utilizing the agrobacterium rhizogenes to obtain stable transgenic hairy roots, rooting can be induced within 1-2 months, the rooting rate is as high as 94.43%, and the transgenic positive rate can reach 98.38%. The invention provides an economical and efficient non-tissue culture dependent transgenic method, it is clear that transgenic roots can be used for gene function verification, the method breaks through the technical obstacle that stable genetic transformation of the carya illinoensis is difficult, and technical support is provided for research of functional genes.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention discloses a granular bio-fertilizer and a preparation method thereof, and the granular bio-fertilizer comprises the following components in percentage by weight: 94-97% of a base fertilizer, 2-5% of a synergist and 1-2% of an auxiliary agent. The granular bio-fertilizer is a fertilizer prepared by compatibility of one of an inorganic fertilizer, an organic fertilizer and an organic and inorganic fertilizer with a bio-fertilizer synergist. The granular bio-fertilizer disclosed by the invention can adopt an inorganic fertilizer, an organic fertilizer or an organic and inorganic fertilizer as a base fertilizer, can be suitable for almost all granular fertilizers (except for fertilizers with a killing function on microorganisms or an antagonistic effect on components in a bio-fertilizer synergist) in the current market, and is simple in production process, relatively low in cost and suitable for industrial production. Moreover, the added value of the product can be effectively improved, and the production process belongs to a novel fertilizer production process and has a huge market popularization value.

The application discloses a nitrogen-containing compound, cross-linked hyaluronic acid and application thereof. Specifically, the application discloses a compound as shown in formula I or a salt thereof. The cross-linked hyaluronic acid prepared by cross-linking hyaluronic acid with the nitrogen-containing compound has good properties and high enzymatic stability, preferably, the enzymatic stability is better than that of a commercial cross-linked hyaluronic acid Durolane.

This invention provides a turbot spleen tissue cell line, with the accession number CCTCCNO:C2022229. The turbot spleen tissue cell line provided by this invention can be continuously passaged, and experimental results show that it remains stable after 65 passages, providing a large number of stable turbot spleen cells for research on immune function genes, etc. The provided cell line has excellent characteristics; it consists of fibroblasts, with spindle-shaped or irregularly triangular cells, several irregular protrusions extending outward from the cell body, and the cells are closely arranged. It can be passaged every 3-4 days, with high recovery efficiency, and can be used for plasmid transfection, siRNA interference, and various other molecular biology experiments.

The present application belongs to the technical field of plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials, cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct organogenesis route. The present application realizes the cyclic production of Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic germination method, and the problems of large offspring trait separation and different qualities caused by the seed asymbiotic germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new technical support for the factory-scale growth of Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and survival rate of seedlings are high.

The present disclosure provides a method for producing biological organic fertilizer under forests. The method includes: culturing the strain of white rot fungus WH‑B‑X3 in the first straw to obtain a first-level solid strain; inoculating the first-level solid strain on the second straw to obtain a second-level solid strain; adding the second-level solid strain to the second liquid strain culture medium in a large fermentation tank to obtain a third-level liquid strain; inoculating the third-level liquid strain on the third straw, and laying the inoculated third straw on the soil under the forest for cultivation to obtain biological organic fertilizer. When the white rot fungus WH‑B‑X3 is used to produce biological organic fertilizer, the straw can be locally sourced, the straw is directly crushed and sprayed in the field, the third straw is obtained, and the inoculation is placed under the forest for aerobic fermentation, and the preparation of biological organic fertilizer can be realized, and the production process is simple, the cost is low, and it is conducive to wide promotion and application.

The invention discloses a preparation method of a decitabine impurity III, and belongs to the field of chemical pharmacy. The decitabine impurity III belongs to a degradable impurity and can be subjected to material conversion in synthesis, transportation and storage processes, and the decitabine impurity III obtained by alkali degradation, separation and purification, desalination and freeze-drying has the advantages of high yield, high purity and simple preparation method. A reliable impurity reference substance can be provided for quality research of decitabine, so that the product quality is ensured, and the medication safety of decitabine is improved.

This invention relates to the field of plant tissue culture, specifically disclosing a method for rapidly propagating dandelion through tissue culture. The method includes the following steps: inducing callus tissue using dandelion leaves as explants; inducing shoot formation from the callus tissue; and rooting the shoots to obtain dandelion tissue culture seedlings. This invention provides a high-quality and efficient tissue culture propagation method for dandelion by utilizing dandelion leaves as explants, inducing callus tissue through dedifferentiation, allowing the callus tissue to redifferentiate and produce shoots, and then rooting individual shoots.

The present invention discloses a new amylase-producing Zobellella species and its application, and relates to the field of microbial technology. The present invention discovers a new microbial species, which is identified as belonging to the genus Zobellella and is named Zobellella sp. MYHB_202302AmY. Experimental verification shows that the bacteria has the ability to tolerate high salt and secrete amylase.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

This invention discloses the application of cotton uORF elements G1u2 and G1u3 in improving gene translation levels, belonging to the field of genetic engineering. This invention utilizes upland cotton... WNK Gene Ghir_D04G003230 Two uORF elements, G1u2 (SEQ ID No. 2) and G1u3 (SEQ ID No. 3), were isolated from cotton. A dual-luciferase reporter assay demonstrated that both G1u2 and G1u3 elements significantly improved the translation efficiency of downstream genes. This invention is the first to discover uORF elements in cotton with a clear positive translation regulatory function, filling a gap in the research and application of positive uORF regulatory elements in cotton. Furthermore, these uORF elements can be applied to molecular design breeding for high-yield, stress-resistant, and high-quality plants, as well as precise gene editing, demonstrating strong practicality.

The invention relates to a method for modifying composite nickel protoxide by using sodium salt, which comprises the following steps: (1) adding iron-containing chloride and nickel chloride solution into a dissolving tank according to the metal proportion corresponding to the product, heating and dissolving to obtain spray pyrolysis pre-solution; the total metal concentration in the liquid before spray pyrolysis is at least maintained at 240 g / L, and the temperature is maintained at 60 DEG C; (2) adding a saturated sodium salt solution into the spray pyrolysis pre-solution, fully and uniformly mixing, and then carrying out spray pyrolysis to obtain a corresponding compound nickel protoxide crude product and a by-product; recovering the by-product for producing a nickel chloride solution; and (3) washing the composite nickel protoxide crude product with hot water, calcining, crushing and then screening to obtain the composite nickel protoxide product. The method is easy to operate, the problem that the apparent density of the composite nickel protoxide product is low is solved, and the character of the composite nickel protoxide is effectively improved. Meanwhile, by-products and waste water generated in the production process can be recycled, and recycling of resources is achieved.

The invention discloses a preparation method of a beta-elemene-containing distant hybridization tobacco material, and belongs to the technical field of plant breeding. According to the method disclosed by the invention, the tobacco material containing beta-elemene, namely the hybrid variety F1094, is created by taking the stem-embraced tobacco as a male parent and taking the Chinese tobacco 300 as a female parent and utilizing a distant hybridization technology. The germination rate of the hybrid F1094 is normal, the survival rate is 100%, and offspring can be fertile; compared with a female parent Zhongyan 300, the hybrid F1094 is higher in plant height, larger in leaf length and leaf width, and contains volatile aroma components such as beta-elemene and phenylacetaldehyde. Experimental results show that according to the preparation method disclosed by the invention, a fertile distant hybridization breeding material containing beta-elemene and phenylacetaldehyde is successfully created, and a new technical support is provided for the improvement of tobacco characters.

The invention provides a bud grafting-based rubber tree breeding new material induction method. The method comprises the following steps: S1, selecting a stock variety and a scion variety; s2, the rootstock variety and the scion variety are rejuvenized; s3, stock and scion preparation; s4, budding; s5, screening of bud grafting mutagenesis materials: phenotype identification screening, stress resistance identification screening or molecular identification screening; according to the method, rubber tree varieties with complementary characters are selected as stocks and scions, so that the overall characters are effectively improved; target traits of the scions are gradually induced to be effectively optimized through multiple budding of the young budlings, and the stability of the traits is improved; through molecular identification screening, adversity stress screening and phenotype identification screening, a target budding mutagenesis plant is rapidly and accurately screened out, the new variety breeding process is accelerated, the breeding period is shortened, and the idea is broadened for creation of new rubber tree breeding materials.

The application discloses a preparation method and production process of a six-membered aromatic ring fused pyrrole compound, and belongs to the field of organic synthesis. The six-membered aromatic ring fused pyrrole compound of the target compound can be obtained in a high yield even under normal pressure by using specific low-boiling-point solvents (a mixed system of toluene and water). In addition, in the post-treatment process after the reaction is completed, a method of sequentially using sodium chloride aqueous solution washing and t-butyl methyl ether beating is used, so that the by-products can be effectively removed in the post-treatment process, and the product properties are improved.

The application discloses a sweet potato cultivation method for improving commercial rate of sweet potato by using condensed ring polyphenol and relates to the technical field of agricultural planting. The method is as follows: a condensed ring polyphenol solution is taken and diluted; when sweet potato seedlings are transplanted, a sweet potato nest is first opened, and the condensed ring polyphenol diluent is poured into the sweet potato nest; after the sweet potato seedling is inserted, the soil is covered and covered tightly. Further, the method can further include: the condensed ring polyphenol diluent is uniformly sprayed during the growth of stems and leaves of the sweet potato. It is proved by experiments that the condensed ring polyphenol diluent used in the application can play a role in regulating the growth of the sweet potato. Specifically, by using the condensed ring polyphenol diluent to pour the nest or to pour the nest and spray, the sweet potato seedling can be early rooted and early reseeded, and the survival rate is improved; the above-mentioned method can also promote the rapid growth of stems and leaves on the ground, increase the number of leaves, the number of branches and the fresh weight, and has a remarkable yield-increasing effect on the number of tubers, the fresh tuber yield and the drying rate.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

The invention discloses an RNAi recombinant vector resistant to both bemisia tabaci and tomato yellow leaf curl virus as well as a construction method and application of the RNAi recombinant vector resistant to both bemisia tabaci and tomato yellow leaf curl virus, a DNA beta promoter accompanied by geminivirus expressed in phloem is constructed to an RNAi vector pCambia 1391 vector, and a 1391:: DNA beta pro vector skeleton is obtained; an RT-PCR (reverse transcription-polymerase chain reaction) method is used for amplifying part of fragments of key genes BtACTB of bemisia tabaci, key segments (including a gene spacer region with virus replication initiation, part of coat protein and replication-related protein) of TYLCV and inverted repeat sequences of the key segments to construct a gene which is driven by a phloem promoter and is capable of expressing the bemisia tabaci gene BtACTB. The recombinant vector is of a reverse hairpin structure and simultaneously targets key sequences of bemisia tabaci and tomato yellow leaf curl virus. The constructed recombinant vector is introduced into tomatoes through an agrobacterium transformation method, and tomato plants capable of reducing the survival rate of bemisia tabaci and inhibiting TYLCV infection are obtained through screening.

The present invention discloses a coconut strong promoter, a plant expression vector and an application thereof, wherein the nucleotide sequence of the promoter is shown in SEQ ID NO. 1. Compared with known promoters, the promoter provided by the present invention can drive the efficient expression of related genes and is a strong promoter. The present invention also relates to a plant expression vector containing the strong promoter and an application thereof in initiating the expression of a target gene.

The invention relates to an efficient multi-character synchronous breeding method for donkeys, which is applied to the field of breeding, and comprises the following steps: selecting a plurality of target characters, and carrying out gene detection and screening, so as to synchronously improve the plurality of characters of the donkeys, and optimizing a breeding scheme by tracking offspring, so that the breeding efficiency is effectively improved, and the breeding cost is reduced. In addition, by tracking and monitoring the offspring donkeys, interference of breeding environment and other germ infection on research of multiple characters of the offspring donkeys is eliminated, the characteristic that the heads of the donkeys wobble when the donkeys get ill is utilized, the conductive touch pressing balls wobble along with the ears, and therefore the breeding period is shortened, and the market demand can be better met. In the process, whether the donkeys get sick or not is judged by analyzing current changes, early warning is sent to workers, the donkeys are checked and confirmed in time, and the survival rate of the offspring donkeys is effectively increased.

This invention belongs to the field of industrial waste resource utilization technology, and discloses a zero-discharge resource utilization method for polysilicon waste liquid. The method involves adding sodium hydroxide solution to chlorosilane waste liquid and reacting it, then obtaining sodium silicate solution through stepwise extraction with dilute alkali or one-step extraction with concentrated alkali. Furthermore, calcium carbide slag is calcined and slaked to obtain lime slurry. Then, the sodium silicate solution and lime solution are added to a reaction vessel according to a specific ratio and heated for reaction. The resulting mixture is filtered to obtain microporous calcium silicate material. The filtrate can be recycled as an alkaline solution for chlorosilane waste liquid. This invention effectively solves the problems of scarce resource utilization pathways and low utilization efficiency of chlorosilane waste liquid by synergistically utilizing chlorosilane waste liquid and calcium carbide slag solid waste. The resulting microporous calcium silicate material has excellent physicochemical properties. Simultaneously, the process is simple and the conditions are mild, achieving comprehensive utilization of industrial waste liquid and residue while also contributing to energy conservation, emission reduction, and source environmental protection.