235 results about "Function genes" patented technology

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for identifying streptococcicosis resistance of tilapia and application of the SNP site combination. DNAs of tilapia tail fin tissues attacked by streptococcus agalactiae are re-sequenced, resistant and sensitive individuals of a tilapia population are screened, GWAS analysis is carried out, SNPs sites related to tilapia streptococcus agalactiae disease resistance are identified, functional gene annotation and enrichment analysis are carried out, two SNP sites LG1320881657 and LG1320548718 are screened, and the SNP sites related to tilapia streptococcus agalactiae disease resistance are obtained. The GG genotype of the LG1320881657 and the TT genotype of the LG1320548718 are the dominant genotypes related to the streptococcus agalactiae disease resistance of the tilapia mossambica. The tilapia mossambica resistant to streptococcosis is successfully screened by utilizing the two SNPs sites, effective SNPs are provided for developing tilapia mossambica anti-streptococcosis molecular marker-assisted breeding, excellent characters of parents are ensured, and the breeding efficiency is improved.

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention provides a plant salt tolerance related transcription factor TaVOZ1 as well as a coding gene and application thereof, and belongs to the technical field of functional genes. The TaVOZ1 is related to plant salt tolerance and has an amino acid sequence as shown in SEQ ID No. 1, and a gene for coding the protein has a nucleotide sequence as shown in SEQ ID No. 2. The plant salt tolerance can be regulated by regulating the content or activity of the protein or the expression quantity of the gene, for example, when the gene is over-expressed, the plant salt tolerance is enhanced, and when the gene is knocked down, the salt tolerance is weakened. The TaVOZ1 disclosed by the invention can be combined with 5 '-CTTCT-3'or a reverse complementary sequence 5'-AAGAAG-3 'in a downstream target gene promoter to activate the expression of a stress response gene so as to improve the salt tolerance of a plant, so that the TaVOZ1 can be used for culturing a salt-tolerant wheat variety.

The invention discloses a sebastes schlegeli testis cell line as well as a construction method and application thereof, and belongs to the technical field of marine fish cell culture. Primary culture and subculture are carried out on sebastes schlegeli juvenile fish testis tissues, so that the sebastes schlegeli testis cell line capable of being continuously subcultured in vitro for a long time is established. The sebastes schlegeli testis cell line established by the invention is in a fibroblast shape, has a good growth curve, and has relatively strong multiplication capacity. The lentiviral vector can significantly express the stromal cell marker gene temp2, can transfect the cell line, and can be subsequently applied to research of gonad development related functional genes. At present, the cell line has been subjected to in-vitro continuous passage for more than 60 generations, can be subjected to cryopreservation, and is high in recovery survival rate. Establishment of the cell line provides a stable and excellent experimental material for research of fish gonad development genes.

The invention discloses a marine fish myoblast in-vitro proliferation method. A basal culture medium is used as a background culture medium of a culture medium; the culture medium contains IGF2 protein with the final concentration of 20-200 ng / mL; the IGF2 protein is derived from plectropomus leopardus. The culture medium is used for culturing the passage cells to obtain the myoblasts proliferated in vitro of the plectropomus leopardus, the in-vitro culture of the myoblasts of the plectropomus leopardus is successfully realized, the number of the myoblasts reaches 30000, and the in-vitro proliferation rate reaches 87%. The invention provides raw materials for exploring the self function of myoblasts and gene regulation and control mechanism, provides preconditions for an in-vitro certification experiment for further exploring the influence of the myoblasts of marine fishes on the growth speed, and also lays a foundation for improved variety breeding of the marine fishes.

The invention relates to the field of forensic medicine, and discloses a method and system for identifying smoking behaviors and application of the method and system. The construction method comprises the following steps: respectively obtaining saliva and excrement of a smoking object and a non-smoking object; performing metagenome sequencing to obtain original microbiome data; after processing the original microbiome data, extracting multi-omics feature information, including species information and gene function annotation information; based on a statistical analysis method, carrying out diversity analysis and screening microbial markers and functional genes significantly related to smoking behaviors; constructing a training set and a test set, and training a machine learning model and model evaluation by taking the microbial markers and / or the functional genes as input features; and performing smoking behavior identification on a target sample by using the model, and outputting a prediction result. The accuracy rate of model identification reaches 0.7966, the sensitivity is 0.8750, the specificity is 0.7037, and the method can be applied to health assessment and judicial expertise.

The invention provides a gene module analysis method and device, equipment and a storage medium, and relates to the technical field of space transcriptomics. The method comprises the steps of obtaining space-time group chip expression data, performing linearization processing, and performing sliding window cutting to obtain a plurality of window units arranged according to a space sequence; calculating gene function activeness and constructing a matrix; clustering and grouping are carried out to obtain window groups; performing difference analysis screening on the window groups to obtain a difference expression gene set; and performing gene module analysis to obtain a functional gene module. According to the method, through an integrated processing flow of structure linearization, sliding window cutting, activeness quantification, clustering analysis and module identification, fine extraction and function reconstruction of spatial expression information in the curled tissue are realized under the condition that an additional algorithm or external data is not introduced; and the practicability and research value of the spatial omics data in a complex organization structure are improved.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention is suitable for the technical field of molecular biology, and provides a rice transcription factor OsGATA16 and application thereof in regulation and control of rice blast resistance of rice. The method comprises the following steps: firstly, cloning a rice OsGATA16 gene, then constructing an overexpression vector and a mutant vector, and transforming rice to obtain a corresponding strain. Research finds that after a mutant strain with OsGATA16 knocked out is inoculated with germs, the number of scabs is obviously smaller than that of a wild type control group, the disease resistance of rice can be accurately regulated and controlled, and the rice blast resistance is obviously improved. The regulation and control mode can effectively reduce pesticide use and reduce environmental pollution and residual risk. Meanwhile, the successfully constructed stable genetic regulation material can be stored for a long time and used for multiple times in molecular and phenotype levels through verification. Besides, the OsGATA16 gene can be used as a disease-resistant molecular marker or a functional gene to be applied to various breeding systems, a new target is provided for breeding new rice materials with high rice blast resistance, and the OsGATA16 gene has a wide practical application prospect.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention belongs to the technical field of molecular marker-assisted breeding, and particularly relates to a molecular marker related to leukoderma resistance on a corn No.8 chromosome and application of the molecular marker, the gene sequence of the molecular marker located on the corn No.8 chromosome is shown as SEQ ID NO: 1 and / or SEQ ID NO: 2, the molecular marker is jointly located to SNP: 8-149153434 and a related gene Zm00001eb358700 (SEQ ID NO: 2) on the corn No.8 chromosome through many-year multi-point experiments, and the molecular marker is used for identifying the leukoderma resistance of the corn No.8 chromosome. The SNP can be positioned in a plurality of different environments, 22.5% of phenotypic variation can be explained, and the highest dominant effect is achieved; the associated candidate functional gene mainly encodes a heat stress transcription factor C-1a of an HSF family and is proved to be related to corn resistance, and the locus can be used as a corn leukoderma resistance molecular marker.

The invention relates to a construction method and application of a plant intra-genus generic transcriptome, and the method is realized by the following steps: obtaining second and third generation transcriptome data of different species in a genus and performing quality control, comparing a reference genome by using software such as HISAT2 and the like, and screening effective transcripts through gffcomplex after StringTie splicing; transcripts and genes are classified based on the ORF length (mRNA is greater than or equal to 100 aa, small peptide transcripts are from 10 aa to 99 aa, and ncRNA is smaller than 10 aa); the method comprises the following steps: analyzing sequence conservative properties among species through coding genes and non-coding genes, and dividing the genes into core, optional and specific types; and finally integrating and constructing a genus-level generic transcriptome containing three types of transcriptomes. According to the invention, the system construction of the plant intra-genus cross-species pan transcriptome is realized for the first time, the conservative property and difference of protein coding genes, small peptides and non-coding RNA can be analyzed, and a new tool is provided for the research of plant functional genomes.

The invention provides a millet heading period regulation gene BBX2 and application thereof, and belongs to the technical field of functional genes. The invention provides a Setaria italica L. Beauv. Heading period regulation gene BBX2. The nucleotide sequence of the gene BBX2 is shown in SEQ ID NO: 2. The gene BBX2 is related to the morning and evening of the heading period of millet. The foxtail millet BBX2 gene editing mutant material is created by agrobacterium mediation, and the result shows that compared with a wild foxtail millet material, the heading period of the foxtail millet BBX2 gene editing mutant material is obviously advanced. The gene BBX2 promotes heading of foxtail millet through negative regulation. The gene BBX2 provided by the invention provides an effective gene locus for screening or constructing a millet variety with an advanced heading stage and developing millet genetic breeding work, and also lays a foundation for large-area popularization and planting of millet.

The invention provides a VIGS silencing system of a hydrangea PDS gene and application of the VIGS silencing system, and belongs to the technical field of gene engineering. The method comprises the following steps: firstly, providing a specific nucleotide fragment of a silent hydrangea PDS gene, and constructing silent vectors pTRV2-HmPDS1 and pTRV2-HmPDS2 on the basis of the specific nucleotide fragment; the method comprises the following steps: respectively transferring vectors pTRV1 and pTRV2, and silencing vectors pTRV2-HmPDS1 and pTRV2-HmPDS2 into agrobacterium to obtain a VIGS silencing system of a hydrangea crispa PDS gene, and infecting a hydrangea crispa plant or a leaf disc by adopting the silencing system; experiments prove that the silencing system disclosed by the invention induces silencing of the hydrangea HmPDS1 and the hydrangea HmPDS2 under the mediation of agrobacterium tumefaciens; the VIGS silencing system provided by the invention can be used for quickly identifying the function of the endogenous gene on the hydrangea plant, is simple and convenient to operate, short in period and low in cost, effectively overcomes the defect that a hydrangea genetic transformation system is imperfect, and provides a technical guarantee for excavation of the hydrangea functional gene.

The present disclosure discloses a Functional Genomic Imaging method. Genes are filtered based on the expression levels and other information first to improve the ratio of effective genes, and then a correlation matrix of gene co-expression is generated based on the filtered gene data to obtain a co-expression network; data conversion is performed based on the correlation matrix of gene co-expression to obtain a distance matrix; and finally, the co-expression network is subjected to dimensionality reduction and cluster analysis, thus obtaining the visualized gene expression according to the analysis result. The Functional Genomic Imaging method provided by the present disclosure achieves a better visualization effect of the genetic co-expression network and can obtain a co-expressed gene cluster more effectively.

The invention discloses a conservative non-coding element identification method and system based on multi-species genome alignment, and the method comprises the following steps: establishing an index database based on reference genome sequences of multiple species, completing whole genome alignment, and further processing to obtain a high-credibility chain alignment result; the multi-species chain type comparison results are integrated into multi-sequence comparison data in a unified format; on this basis, a neutral evolution model is constructed based on quadruple degenerate sites, and candidate conservative regions are predicted through conservative scoring; in combination with genome annotation information, a length threshold is set, and a coding region and a UTR region are rejected, so that a high-confidence non-coding conservative element is obtained; and finally, displaying a cross-species conservative distribution diagram of the CNE by utilizing a visual tool. According to the method, the CNE with a potential regulation function can be accurately, efficiently and automatically identified, and technical support is provided for regulation system analysis, functional gene mining and molecular breeding of various organisms.

The present invention concerns selective renal denervation treatment of drug resistant hypertensive patients by correlating the patients' genetic panel by categorization and hierarchy according to patients' genetic variants within the functional genes for heart activity, for the renin-angiotensin aldosterone system, and for renal activity.

The invention relates to the technical field of gene prediction, and particularly discloses a functional gene prediction method based on deep learning, which comprises the following steps of: 1, detecting physiological characteristics of a target individual so as to judge whether a target functional gene is expressed or not and judge the expression degree; 2, analyzing the relationship between the environment and the expression of the target functional gene so as to judge whether the change of the environment has an influence on the expression of the target functional gene or not; by analyzing the relationship between the environment and the expression of the target functional gene, how the environmental factors influence the expression of the gene can be revealed, so that the interaction mechanism of the gene and the environment is deeply understood, a corresponding prevention strategy is formulated, the expression of the gene is regulated and controlled by changing the environmental factors, the accuracy of gene mining and identification is improved, and the application prospect is wide. And a powerful tool is provided for further deeply researching the action mechanism of the gene function.

The invention relates to the technical field of medical assistance, in particular to a depression risk assessment method and system based on intestinal flora characteristics. The method comprises the following steps: acquiring an individual enteric microorganism sample, performing nucleic acid sequencing on the enteric microorganism sample, and determining abundance data of functional genes related to a neural activity metabolic pathway in enteric microorganisms; carrying out metabolite detection on the intestinal microorganism sample, and determining concentration data of metabolites related to the neural activity metabolic pathway in the intestinal microorganisms; based on the abundance data of the functional genes and the concentration data of the metabolites, data fusion processing is carried out, a neural activity metabolism function spectrum is constructed, and the neural activity metabolism function spectrum is a multi-dimensional feature vector; and inputting the neural activity metabolic function spectrum into a pre-trained risk assessment model, and outputting a depression risk score of the individual. The accuracy and repeatability of evaluation are remarkably improved, and the method has great potential for early screening, dynamic monitoring and personalized health management.

The invention discloses a method for inducing apocarya stems to generate transgenic hairy roots, and belongs to the field of forest tree genetic engineering. According to the method, the semi-lignified or lignified stems of the apocarya are infected by utilizing the agrobacterium rhizogenes to obtain stable transgenic hairy roots, rooting can be induced within 1-2 months, the rooting rate is as high as 94.43%, and the transgenic positive rate can reach 98.38%. The invention provides an economical and efficient non-tissue culture dependent transgenic method, it is clear that transgenic roots can be used for gene function verification, the method breaks through the technical obstacle that stable genetic transformation of the carya illinoensis is difficult, and technical support is provided for research of functional genes.

The invention belongs to the field of molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig backfat thickness character and application thereof. According to the invention, an SNP (Single Nucleotide Polymorphism) site g.348Agt related to the back fat thickness character of the pig is screened in an intron region of the ADIPOQ gene; the SNP site is located at the 348bp nucleotide at the downstream of the ADIPOQ gene transcription start site, and the polymorphism of the SNP site is A or G. According to the present invention, the pig backfat thickness condition is determined according to the genotype of the mutation site, such that the functional gene and the molecular genetic marker related to the pig backfat thickness are obtained, and the dominant allele of the SNP molecular marker is optimized and the site is rapidly detected so as to determine the pig backfat thickness, reference basis can be provided for accurate evaluation of pig fat deposition capacity regulation related SNP molecular marker assisted breeding, and meanwhile, the method has important significance on pig strain or variety improvement.

The application discloses SNP molecular markers closely linked to lotus powder brittle traits, a KASP detection primer group and application. A main QTL site for controlling lotus powder brittle traits is obtained by using SLAF-seq to develop a large number of SNP polymorphism markers, the main QTL site is closely linked to the SNP molecular markers, has a high contribution rate to lotus powder brittle traits, is used for map-based cloning to mine functional genes for controlling powder brittle early or late and molecular marker assisted selection, the application provides SNP molecular markers closely linked to two main QTL sites for controlling lotus powder brittle traits and designs a KASP detection primer group with high specificity and high accuracy, and the KASP detection primer group or a detection kit containing the detection primer group can be used for specifically and accurately identifying lotus powder or brittle traits or genotypes, and has application prospects in the selection or identification of lotus powder or brittle varieties and lotus powder brittle molecular marker assisted breeding.

The present invention provides an application of a Zm00001d030087 gene in regulating the protein content of corn kernels, and relates to the field of agricultural biotechnology. The Zm00001d030087 gene sequence is shown in SEQ ID NO: 1, and the gene is determined to be a functional gene for regulating the protein content of kernels. The gene can be used to regulate the protein content of corn kernels, and can provide a basis for breeding corn varieties with high protein content.

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

The application discloses RhALEU protein and an application of an encoding gene thereof in regulation of old age of rose petals. RhALEU The application screens out a gene from a rose transcriptome database RhALEU , and finds that expression of the gene is induced in the whole petal development stage. RhALEU The application verifies that the gene has a function of promoting old age of rose petals by using VIGS technology. RhALEU Silencing of the gene slows down the speed of the petal from the 5th grade to losing ornamental value. RhALEU In the petal with the silenced gene, expression of the old age related gene SAG12 is significantly down-regulated. RhALEU The application discloses the function of the gene in natural old age of the petal, provides a new thought and technical means for regulating old age of a plant flower, especially postharvest preservation of fresh cut rose, opens up a new research field of flower regulation, and has great academic value and important production application prospect.

The invention discloses a plant leaf globulin BrLH1 coding gene, and belongs to the field of plant genes. According to the invention, a specific primer is designed, a PCR technology is utilized, and a gene named as BrLH1 is cloned from comfortable heading Chinese cabbage; the qPCR analysis finds that the gene is expressed in a Chinese cabbage flat-collapse non-heading mutant, but the expression of the gene is lower than that of a wild type. Through MutMap result analysis, the BrLH1 (leafy head 1) is a function deletion type gene, and after the gene BrmLH1 is mutated, the leaf of the Chinese cabbage does not form a ball. The BrLH1 can be used as a gene resource for improving plant morphology, can realize precise design and breeding of plant gene editing, and has important theoretical significance and application value in the aspects of creating new germplasm and improving yield and quality.

This invention provides a multi-omics diagnostic model for inflammatory bowel disease (IBD), along with its biomarker screening method, applications, and reagent kits. Currently, there is no gold standard for diagnosing IBD, leading to many unidentifiable suspected cases in clinical practice. This invention utilizes information on the abundance of gut bacteria, the functional genes of gut bacteria, and the content of intestinal metabolites to establish a predictive model for IBD. Furthermore, this invention integrates the above two types of omics information and the prediction across three information dimensions to establish a predictive model with impressive predictive capabilities. The method and model of this invention can assist in the diagnosis and differentiation of IBD.

This invention discloses the application of IKK inhibitors in the preparation of drugs to restore pancreatic islet function. IKK inhibitors can effectively improve the problem of pancreatic β-cell differentiation defects, significantly increase the number of pancreatic β-cells, enhance the insulin secretion capacity of islet cells, and specifically upregulate the expression of key functional genes of pancreatic β-cells such as INS and NKX6.1 genes, thus repairing pancreatic islet function damage. This solves the technical problem that current treatments for type 1 diabetes can only replace insulin and relieve blood sugar symptoms, but cannot fundamentally repair pancreatic islet function. IKK inhibitors can be used to prepare drugs for the treatment of type 1 diabetes, providing a new approach for the clinical treatment of type 1 diabetes and possessing extremely high clinical translational value.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The present invention discloses a Cre recombinase regulatory system and its application, wherein the Cre recombinase regulatory system includes the ER50 gene. The present invention constructs a Cre recombinase regulatory system by introducing 4OHT to induce degradation of the protein ER50. In the Cre recombinase regulatory system constructed by the present invention, 4OHT achieves more stringent regulation of the functional gene Cre through dual regulation of ER50 and ERT2, reducing background issues in the Cre system. The application of this system will enable more rigorous spatiotemporal regulation of gene expression in cells and animals in the future, providing a more reliable research tool for conditional gene knockout in cells and animals.