194 results about "Function genes" patented technology

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention provides a plant salt tolerance related transcription factor TaVOZ1 as well as a coding gene and application thereof, and belongs to the technical field of functional genes. The TaVOZ1 is related to plant salt tolerance and has an amino acid sequence as shown in SEQ ID No. 1, and a gene for coding the protein has a nucleotide sequence as shown in SEQ ID No. 2. The plant salt tolerance can be regulated by regulating the content or activity of the protein or the expression quantity of the gene, for example, when the gene is over-expressed, the plant salt tolerance is enhanced, and when the gene is knocked down, the salt tolerance is weakened. The TaVOZ1 disclosed by the invention can be combined with 5 '-CTTCT-3'or a reverse complementary sequence 5'-AAGAAG-3 'in a downstream target gene promoter to activate the expression of a stress response gene so as to improve the salt tolerance of a plant, so that the TaVOZ1 can be used for culturing a salt-tolerant wheat variety.

The invention relates to the field of forensic medicine, and discloses a method and system for identifying smoking behaviors and application of the method and system. The construction method comprises the following steps: respectively obtaining saliva and excrement of a smoking object and a non-smoking object; performing metagenome sequencing to obtain original microbiome data; after processing the original microbiome data, extracting multi-omics feature information, including species information and gene function annotation information; based on a statistical analysis method, carrying out diversity analysis and screening microbial markers and functional genes significantly related to smoking behaviors; constructing a training set and a test set, and training a machine learning model and model evaluation by taking the microbial markers and / or the functional genes as input features; and performing smoking behavior identification on a target sample by using the model, and outputting a prediction result. The accuracy rate of model identification reaches 0.7966, the sensitivity is 0.8750, the specificity is 0.7037, and the method can be applied to health assessment and judicial expertise.

The invention provides a gene module analysis method and device, equipment and a storage medium, and relates to the technical field of space transcriptomics. The method comprises the steps of obtaining space-time group chip expression data, performing linearization processing, and performing sliding window cutting to obtain a plurality of window units arranged according to a space sequence; calculating gene function activeness and constructing a matrix; clustering and grouping are carried out to obtain window groups; performing difference analysis screening on the window groups to obtain a difference expression gene set; and performing gene module analysis to obtain a functional gene module. According to the method, through an integrated processing flow of structure linearization, sliding window cutting, activeness quantification, clustering analysis and module identification, fine extraction and function reconstruction of spatial expression information in the curled tissue are realized under the condition that an additional algorithm or external data is not introduced; and the practicability and research value of the spatial omics data in a complex organization structure are improved.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention is suitable for the technical field of molecular biology, and provides a rice transcription factor OsGATA16 and application thereof in regulation and control of rice blast resistance of rice. The method comprises the following steps: firstly, cloning a rice OsGATA16 gene, then constructing an overexpression vector and a mutant vector, and transforming rice to obtain a corresponding strain. Research finds that after a mutant strain with OsGATA16 knocked out is inoculated with germs, the number of scabs is obviously smaller than that of a wild type control group, the disease resistance of rice can be accurately regulated and controlled, and the rice blast resistance is obviously improved. The regulation and control mode can effectively reduce pesticide use and reduce environmental pollution and residual risk. Meanwhile, the successfully constructed stable genetic regulation material can be stored for a long time and used for multiple times in molecular and phenotype levels through verification. Besides, the OsGATA16 gene can be used as a disease-resistant molecular marker or a functional gene to be applied to various breeding systems, a new target is provided for breeding new rice materials with high rice blast resistance, and the OsGATA16 gene has a wide practical application prospect.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention relates to a construction method and application of a plant intra-genus generic transcriptome, and the method is realized by the following steps: obtaining second and third generation transcriptome data of different species in a genus and performing quality control, comparing a reference genome by using software such as HISAT2 and the like, and screening effective transcripts through gffcomplex after StringTie splicing; transcripts and genes are classified based on the ORF length (mRNA is greater than or equal to 100 aa, small peptide transcripts are from 10 aa to 99 aa, and ncRNA is smaller than 10 aa); the method comprises the following steps: analyzing sequence conservative properties among species through coding genes and non-coding genes, and dividing the genes into core, optional and specific types; and finally integrating and constructing a genus-level generic transcriptome containing three types of transcriptomes. According to the invention, the system construction of the plant intra-genus cross-species pan transcriptome is realized for the first time, the conservative property and difference of protein coding genes, small peptides and non-coding RNA can be analyzed, and a new tool is provided for the research of plant functional genomes.

The invention provides a millet heading period regulation gene BBX2 and application thereof, and belongs to the technical field of functional genes. The invention provides a Setaria italica L. Beauv. Heading period regulation gene BBX2. The nucleotide sequence of the gene BBX2 is shown in SEQ ID NO: 2. The gene BBX2 is related to the morning and evening of the heading period of millet. The foxtail millet BBX2 gene editing mutant material is created by agrobacterium mediation, and the result shows that compared with a wild foxtail millet material, the heading period of the foxtail millet BBX2 gene editing mutant material is obviously advanced. The gene BBX2 promotes heading of foxtail millet through negative regulation. The gene BBX2 provided by the invention provides an effective gene locus for screening or constructing a millet variety with an advanced heading stage and developing millet genetic breeding work, and also lays a foundation for large-area popularization and planting of millet.

The invention provides a VIGS silencing system of a hydrangea PDS gene and application of the VIGS silencing system, and belongs to the technical field of gene engineering. The method comprises the following steps: firstly, providing a specific nucleotide fragment of a silent hydrangea PDS gene, and constructing silent vectors pTRV2-HmPDS1 and pTRV2-HmPDS2 on the basis of the specific nucleotide fragment; the method comprises the following steps: respectively transferring vectors pTRV1 and pTRV2, and silencing vectors pTRV2-HmPDS1 and pTRV2-HmPDS2 into agrobacterium to obtain a VIGS silencing system of a hydrangea crispa PDS gene, and infecting a hydrangea crispa plant or a leaf disc by adopting the silencing system; experiments prove that the silencing system disclosed by the invention induces silencing of the hydrangea HmPDS1 and the hydrangea HmPDS2 under the mediation of agrobacterium tumefaciens; the VIGS silencing system provided by the invention can be used for quickly identifying the function of the endogenous gene on the hydrangea plant, is simple and convenient to operate, short in period and low in cost, effectively overcomes the defect that a hydrangea genetic transformation system is imperfect, and provides a technical guarantee for excavation of the hydrangea functional gene.

The present disclosure discloses a Functional Genomic Imaging method. Genes are filtered based on the expression levels and other information first to improve the ratio of effective genes, and then a correlation matrix of gene co-expression is generated based on the filtered gene data to obtain a co-expression network; data conversion is performed based on the correlation matrix of gene co-expression to obtain a distance matrix; and finally, the co-expression network is subjected to dimensionality reduction and cluster analysis, thus obtaining the visualized gene expression according to the analysis result. The Functional Genomic Imaging method provided by the present disclosure achieves a better visualization effect of the genetic co-expression network and can obtain a co-expressed gene cluster more effectively.

The invention discloses a conservative non-coding element identification method and system based on multi-species genome alignment, and the method comprises the following steps: establishing an index database based on reference genome sequences of multiple species, completing whole genome alignment, and further processing to obtain a high-credibility chain alignment result; the multi-species chain type comparison results are integrated into multi-sequence comparison data in a unified format; on this basis, a neutral evolution model is constructed based on quadruple degenerate sites, and candidate conservative regions are predicted through conservative scoring; in combination with genome annotation information, a length threshold is set, and a coding region and a UTR region are rejected, so that a high-confidence non-coding conservative element is obtained; and finally, displaying a cross-species conservative distribution diagram of the CNE by utilizing a visual tool. According to the method, the CNE with a potential regulation function can be accurately, efficiently and automatically identified, and technical support is provided for regulation system analysis, functional gene mining and molecular breeding of various organisms.

The present invention concerns selective renal denervation treatment of drug resistant hypertensive patients by correlating the patients' genetic panel by categorization and hierarchy according to patients' genetic variants within the functional genes for heart activity, for the renin-angiotensin aldosterone system, and for renal activity.

The invention relates to the technical field of medical assistance, in particular to a depression risk assessment method and system based on intestinal flora characteristics. The method comprises the following steps: acquiring an individual enteric microorganism sample, performing nucleic acid sequencing on the enteric microorganism sample, and determining abundance data of functional genes related to a neural activity metabolic pathway in enteric microorganisms; carrying out metabolite detection on the intestinal microorganism sample, and determining concentration data of metabolites related to the neural activity metabolic pathway in the intestinal microorganisms; based on the abundance data of the functional genes and the concentration data of the metabolites, data fusion processing is carried out, a neural activity metabolism function spectrum is constructed, and the neural activity metabolism function spectrum is a multi-dimensional feature vector; and inputting the neural activity metabolic function spectrum into a pre-trained risk assessment model, and outputting a depression risk score of the individual. The accuracy and repeatability of evaluation are remarkably improved, and the method has great potential for early screening, dynamic monitoring and personalized health management.

The application discloses SNP molecular markers closely linked to lotus powder brittle traits, a KASP detection primer group and application. A main QTL site for controlling lotus powder brittle traits is obtained by using SLAF-seq to develop a large number of SNP polymorphism markers, the main QTL site is closely linked to the SNP molecular markers, has a high contribution rate to lotus powder brittle traits, is used for map-based cloning to mine functional genes for controlling powder brittle early or late and molecular marker assisted selection, the application provides SNP molecular markers closely linked to two main QTL sites for controlling lotus powder brittle traits and designs a KASP detection primer group with high specificity and high accuracy, and the KASP detection primer group or a detection kit containing the detection primer group can be used for specifically and accurately identifying lotus powder or brittle traits or genotypes, and has application prospects in the selection or identification of lotus powder or brittle varieties and lotus powder brittle molecular marker assisted breeding.

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

The application discloses RhALEU protein and an application of an encoding gene thereof in regulation of old age of rose petals. RhALEU The application screens out a gene from a rose transcriptome database RhALEU , and finds that expression of the gene is induced in the whole petal development stage. RhALEU The application verifies that the gene has a function of promoting old age of rose petals by using VIGS technology. RhALEU Silencing of the gene slows down the speed of the petal from the 5th grade to losing ornamental value. RhALEU In the petal with the silenced gene, expression of the old age related gene SAG12 is significantly down-regulated. RhALEU The application discloses the function of the gene in natural old age of the petal, provides a new thought and technical means for regulating old age of a plant flower, especially postharvest preservation of fresh cut rose, opens up a new research field of flower regulation, and has great academic value and important production application prospect.

The invention discloses a plant leaf globulin BrLH1 coding gene, and belongs to the field of plant genes. According to the invention, a specific primer is designed, a PCR technology is utilized, and a gene named as BrLH1 is cloned from comfortable heading Chinese cabbage; the qPCR analysis finds that the gene is expressed in a Chinese cabbage flat-collapse non-heading mutant, but the expression of the gene is lower than that of a wild type. Through MutMap result analysis, the BrLH1 (leafy head 1) is a function deletion type gene, and after the gene BrmLH1 is mutated, the leaf of the Chinese cabbage does not form a ball. The BrLH1 can be used as a gene resource for improving plant morphology, can realize precise design and breeding of plant gene editing, and has important theoretical significance and application value in the aspects of creating new germplasm and improving yield and quality.

This invention provides a multi-omics diagnostic model for inflammatory bowel disease (IBD), along with its biomarker screening method, applications, and reagent kits. Currently, there is no gold standard for diagnosing IBD, leading to many unidentifiable suspected cases in clinical practice. This invention utilizes information on the abundance of gut bacteria, the functional genes of gut bacteria, and the content of intestinal metabolites to establish a predictive model for IBD. Furthermore, this invention integrates the above two types of omics information and the prediction across three information dimensions to establish a predictive model with impressive predictive capabilities. The method and model of this invention can assist in the diagnosis and differentiation of IBD.

This invention discloses the application of IKK inhibitors in the preparation of drugs to restore pancreatic islet function. IKK inhibitors can effectively improve the problem of pancreatic β-cell differentiation defects, significantly increase the number of pancreatic β-cells, enhance the insulin secretion capacity of islet cells, and specifically upregulate the expression of key functional genes of pancreatic β-cells such as INS and NKX6.1 genes, thus repairing pancreatic islet function damage. This solves the technical problem that current treatments for type 1 diabetes can only replace insulin and relieve blood sugar symptoms, but cannot fundamentally repair pancreatic islet function. IKK inhibitors can be used to prepare drugs for the treatment of type 1 diabetes, providing a new approach for the clinical treatment of type 1 diabetes and possessing extremely high clinical translational value.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The present invention discloses a Cre recombinase regulatory system and its application, wherein the Cre recombinase regulatory system includes the ER50 gene. The present invention constructs a Cre recombinase regulatory system by introducing 4OHT to induce degradation of the protein ER50. In the Cre recombinase regulatory system constructed by the present invention, 4OHT achieves more stringent regulation of the functional gene Cre through dual regulation of ER50 and ERT2, reducing background issues in the Cre system. The application of this system will enable more rigorous spatiotemporal regulation of gene expression in cells and animals in the future, providing a more reliable research tool for conditional gene knockout in cells and animals.

The present invention discloses a SNP molecular marker combination of alfalfa and its application. The SNP molecular marker combination consists of 61,351 SNP molecular markers. The physical positions of the 61,351 SNP molecular markers are determined by sequence alignment based on the alfalfa reference genome ZM-4 alfalfa genome. The SNP molecular marker sites of the present invention have good site specificity, strong universality, and high polymorphism, and can effectively distinguish alfalfa germplasms from different sources. Preparing the SNP molecular markers of the present invention into a whole-genome liquid gene chip for alfalfa can be used for genetic diversity assessment of alfalfa germplasm resources, construction of genetic maps and functional gene mapping, genome-wide association analysis, etc., providing important tools and technical support for molecular design breeding of alfalfa and identification of variety authenticity, and having important significance for the high-quality development of the alfalfa seed industry in China.

The invention discloses a fructus gardeniae and szechwan chinaberry fruit composition and application thereof in liver protection, and belongs to the technical field of biological medicines. The invention provides a cape jasmine fruit and szechwan chinaberry fruit composition, and the proportion of cape jasmine fruit to szechwan chinaberry fruit is (1-2): (1-6), optimally 2: 3. The cape jasmine and szechwan chinaberry fruit composition can be used as an activating agent of DBP; experiments prove that after overexpression of the DBP, the expression quantities of metabolic genes Cyp2a4, ALB, G6PC and Sult1c1 are remarkably increased, which indicates that overexpression of the DBP can promote liver functions. The fructus gardeniae and szechwan chinaberry fruit composition or overexpression DBP provided by the invention can play the role of resisting oxidation and protecting the liver by adjusting an antioxidant function gene, a new thought and strategy are provided for treatment of liver diseases, and an important scientific basis is provided for development of a natural small-molecule DBP agonist.

The application belongs to the field of plant molecular breeding, and specifically discloses haplotype identification markers of a rice fertility restoration gene Rf4 Based on the high-quality rice reference genome sequences of 33 different varieties published by Professor Qin Peng's team of Sichuan Agricultural University, the published gene sequences or functional gene sites are compared to distinguish the varieties containing target genes, and then the bioinformatics method is used to quickly develop a haplotype identification marker combination of a rice fertility restoration gene Rf4 .

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The invention relates to a multi-technology fusion rapid breeding method based on exogenous frame-free wheat homozygous mutants, and belongs to the technical field of wheat genetic breeding. The method comprises the following steps: determining a target gene (such as an LCY gene), creating a homozygous mutant by utilizing a CRISPR-Cas9 gene editing technology, separating an exogenous Cas9 editing frame through hybridization and PCR detection, developing a KASP molecular marker to track a mutation type, and performing phenotype analysis. According to the method, gene editing, cross breeding and molecular detection technologies are fused, the problems that traditional breeding is long in period and foreign genes are difficult to remove are solved, rapid and accurate wheat germplasm creation is achieved, and an efficient way is provided for functional gene utilization.

The application relates to application of a qCTS11.1 gene in regulating cold tolerance of rice, and a method for improving cold tolerance of rice, comprising the steps of replacing the original qCTS11.1 gene in the genome of a starting rice variety with a low-function qCTS11.1 gene, or knocking out the qCTS11.1 gene from the genome of the starting rice variety. The application finds a new cold tolerance related gene qCTS11.1, provides a theoretical basis and gene resource for rice cold tolerance improvement and breeding, and can be used for hybrid breeding and transgenic breeding.

The present invention discloses a method for inducing transgenic hairy roots in the stems of Carya serrata, belonging to the field of forest genetic engineering. The method utilizes Agrobacterium rhizogenes to infect semi-lignified or lignified stems of Carya serrata to obtain stable transgenic hairy roots. These roots can be induced to develop within one to two months, with a rooting rate as high as 94.43% and a transgene-positive rate of 98.38%. This method provides a cost-effective, tissue culture-independent transgenic method, and clearly demonstrates that transgenic roots can be used to verify gene function. This method overcomes the technical barriers to stable genetic transformation in Carya serrata, providing technical support for the research of functional genes.