137 results about "Function genes" patented technology

The invention provides a gene module analysis method and device, equipment and a storage medium, and relates to the technical field of space transcriptomics. The method comprises the steps of obtaining space-time group chip expression data, performing linearization processing, and performing sliding window cutting to obtain a plurality of window units arranged according to a space sequence; calculating gene function activeness and constructing a matrix; clustering and grouping are carried out to obtain window groups; performing difference analysis screening on the window groups to obtain a difference expression gene set; and performing gene module analysis to obtain a functional gene module. According to the method, through an integrated processing flow of structure linearization, sliding window cutting, activeness quantification, clustering analysis and module identification, fine extraction and function reconstruction of spatial expression information in the curled tissue are realized under the condition that an additional algorithm or external data is not introduced; and the practicability and research value of the spatial omics data in a complex organization structure are improved.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention discloses a conservative non-coding element identification method and system based on multi-species genome alignment, and the method comprises the following steps: establishing an index database based on reference genome sequences of multiple species, completing whole genome alignment, and further processing to obtain a high-credibility chain alignment result; the multi-species chain type comparison results are integrated into multi-sequence comparison data in a unified format; on this basis, a neutral evolution model is constructed based on quadruple degenerate sites, and candidate conservative regions are predicted through conservative scoring; in combination with genome annotation information, a length threshold is set, and a coding region and a UTR region are rejected, so that a high-confidence non-coding conservative element is obtained; and finally, displaying a cross-species conservative distribution diagram of the CNE by utilizing a visual tool. According to the method, the CNE with a potential regulation function can be accurately, efficiently and automatically identified, and technical support is provided for regulation system analysis, functional gene mining and molecular breeding of various organisms.

The invention relates to the technical field of medical assistance, in particular to a depression risk assessment method and system based on intestinal flora characteristics. The method comprises the following steps: acquiring an individual enteric microorganism sample, performing nucleic acid sequencing on the enteric microorganism sample, and determining abundance data of functional genes related to a neural activity metabolic pathway in enteric microorganisms; carrying out metabolite detection on the intestinal microorganism sample, and determining concentration data of metabolites related to the neural activity metabolic pathway in the intestinal microorganisms; based on the abundance data of the functional genes and the concentration data of the metabolites, data fusion processing is carried out, a neural activity metabolism function spectrum is constructed, and the neural activity metabolism function spectrum is a multi-dimensional feature vector; and inputting the neural activity metabolic function spectrum into a pre-trained risk assessment model, and outputting a depression risk score of the individual. The accuracy and repeatability of evaluation are remarkably improved, and the method has great potential for early screening, dynamic monitoring and personalized health management.

The application discloses SNP molecular markers closely linked to lotus powder brittle traits, a KASP detection primer group and application. A main QTL site for controlling lotus powder brittle traits is obtained by using SLAF-seq to develop a large number of SNP polymorphism markers, the main QTL site is closely linked to the SNP molecular markers, has a high contribution rate to lotus powder brittle traits, is used for map-based cloning to mine functional genes for controlling powder brittle early or late and molecular marker assisted selection, the application provides SNP molecular markers closely linked to two main QTL sites for controlling lotus powder brittle traits and designs a KASP detection primer group with high specificity and high accuracy, and the KASP detection primer group or a detection kit containing the detection primer group can be used for specifically and accurately identifying lotus powder or brittle traits or genotypes, and has application prospects in the selection or identification of lotus powder or brittle varieties and lotus powder brittle molecular marker assisted breeding.

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

The application discloses RhALEU protein and an application of an encoding gene thereof in regulation of old age of rose petals. RhALEU The application screens out a gene from a rose transcriptome database RhALEU , and finds that expression of the gene is induced in the whole petal development stage. RhALEU The application verifies that the gene has a function of promoting old age of rose petals by using VIGS technology. RhALEU Silencing of the gene slows down the speed of the petal from the 5th grade to losing ornamental value. RhALEU In the petal with the silenced gene, expression of the old age related gene SAG12 is significantly down-regulated. RhALEU The application discloses the function of the gene in natural old age of the petal, provides a new thought and technical means for regulating old age of a plant flower, especially postharvest preservation of fresh cut rose, opens up a new research field of flower regulation, and has great academic value and important production application prospect.

This invention provides a multi-omics diagnostic model for inflammatory bowel disease (IBD), along with its biomarker screening method, applications, and reagent kits. Currently, there is no gold standard for diagnosing IBD, leading to many unidentifiable suspected cases in clinical practice. This invention utilizes information on the abundance of gut bacteria, the functional genes of gut bacteria, and the content of intestinal metabolites to establish a predictive model for IBD. Furthermore, this invention integrates the above two types of omics information and the prediction across three information dimensions to establish a predictive model with impressive predictive capabilities. The method and model of this invention can assist in the diagnosis and differentiation of IBD.

This invention discloses the application of IKK inhibitors in the preparation of drugs to restore pancreatic islet function. IKK inhibitors can effectively improve the problem of pancreatic β-cell differentiation defects, significantly increase the number of pancreatic β-cells, enhance the insulin secretion capacity of islet cells, and specifically upregulate the expression of key functional genes of pancreatic β-cells such as INS and NKX6.1 genes, thus repairing pancreatic islet function damage. This solves the technical problem that current treatments for type 1 diabetes can only replace insulin and relieve blood sugar symptoms, but cannot fundamentally repair pancreatic islet function. IKK inhibitors can be used to prepare drugs for the treatment of type 1 diabetes, providing a new approach for the clinical treatment of type 1 diabetes and possessing extremely high clinical translational value.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The invention discloses a fructus gardeniae and szechwan chinaberry fruit composition and application thereof in liver protection, and belongs to the technical field of biological medicines. The invention provides a cape jasmine fruit and szechwan chinaberry fruit composition, and the proportion of cape jasmine fruit to szechwan chinaberry fruit is (1-2): (1-6), optimally 2: 3. The cape jasmine and szechwan chinaberry fruit composition can be used as an activating agent of DBP; experiments prove that after overexpression of the DBP, the expression quantities of metabolic genes Cyp2a4, ALB, G6PC and Sult1c1 are remarkably increased, which indicates that overexpression of the DBP can promote liver functions. The fructus gardeniae and szechwan chinaberry fruit composition or overexpression DBP provided by the invention can play the role of resisting oxidation and protecting the liver by adjusting an antioxidant function gene, a new thought and strategy are provided for treatment of liver diseases, and an important scientific basis is provided for development of a natural small-molecule DBP agonist.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The invention relates to a multi-technology fusion rapid breeding method based on exogenous frame-free wheat homozygous mutants, and belongs to the technical field of wheat genetic breeding. The method comprises the following steps: determining a target gene (such as an LCY gene), creating a homozygous mutant by utilizing a CRISPR-Cas9 gene editing technology, separating an exogenous Cas9 editing frame through hybridization and PCR detection, developing a KASP molecular marker to track a mutation type, and performing phenotype analysis. According to the method, gene editing, cross breeding and molecular detection technologies are fused, the problems that traditional breeding is long in period and foreign genes are difficult to remove are solved, rapid and accurate wheat germplasm creation is achieved, and an efficient way is provided for functional gene utilization.

The application relates to application of a qCTS11.1 gene in regulating cold tolerance of rice, and a method for improving cold tolerance of rice, comprising the steps of replacing the original qCTS11.1 gene in the genome of a starting rice variety with a low-function qCTS11.1 gene, or knocking out the qCTS11.1 gene from the genome of the starting rice variety. The application finds a new cold tolerance related gene qCTS11.1, provides a theoretical basis and gene resource for rice cold tolerance improvement and breeding, and can be used for hybrid breeding and transgenic breeding.

The invention belongs to the technical field of genetic engineering. The invention provides a gene editing technology system based on barley variety flower 22 immature embryo transformation, which comprises the following steps: (1) taking barley immature embryos as explants, carrying out pretreatment, and optimizing infection and screening conditions in an agrobacterium tumefaciens transformation process; (2) designing aiming at an exon segment of a target gene to obtain a target sequence sgRNA, designing and synthesizing HvPDSCrp-F and HvPDSCrp-R by utilizing the target sequence sgRNA, and then constructing a TaU3-CAS9-pds gene editing vector; and (3) infecting barley immature embryos by using an agrobacterium mixed bacterium solution containing the gene editing carrier in the step (2), then carrying out induced culture, differentiating regenerated seedlings, and inducing rooting to obtain gene editing plants. The technical scheme provides a powerful way for functional gene mining, germplasm innovation and the like in barley.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

The invention belongs to the technical field of gene mining, and particularly relates to a multi-level evidence and artificial intelligence character key gene mining method and system, and the method comprises the steps: S1, constructing a multi-omics database and integrating an interaction network; s2, predicting by using different machine learning algorithms; s3, constructing and applying an online platform: developing the online platform based on an integration result, and integrating a part of known interaction relationship databases; and systematically analyzing a genetic network of plant height, grains and drought resistance characters, and mining key genes. According to the method, the interaction network and multi-omics data are integrated, the mutual relation between functional genes is enriched, and all related genes of target characters are mined. And meanwhile, quantitative integration is performed on the relationship of the genes by utilizing artificial intelligence, and the importance of the related genes can be ranked. By means of the method, all related genes of the target character can be found out, key genes can be verified preferentially according to importance, and the method has important guiding significance on target character inheritance and molecular analysis.

This invention belongs to the field of genetic engineering and discloses genes for rice appearance quality. OsGRL Applications of genetic engineering. This invention first provides rice genes. OsGRL The cDNA sequence of this gene is shown in SEQ ID NO.1, and the coding region sequence is shown in SEQ ID NO.2. This invention also provides a rice gene. OsGRL Gene editing vectors, and provide rice genes OsGRL and Rice genes OsGRL The application of gene-editing vectors in regulating rice grain length. OsGRL This invention can increase rice grain length by editing loss-of-function genes in plants. OsGRL Genes can be used for the genetic improvement of rice grain length and quality traits.

The invention discloses screening and application of a fluorescent quantitative reference gene in a root and stem development process of iris vetiveria, and belongs to the technical field of plant genetic engineering. By constructing a strict reference gene evaluation and screening system, the reference gene with the most stable expression in different stages of root and stem development of iris vetiveria is screened out from eight candidate genes for the first time, errors in samples and between samples caused by internal reference selection are remarkably reduced, accurate correction and standardization of the expression level of a target gene are achieved, and the accuracy of the reference gene is improved. And reliable technical guarantee is provided for subsequent large-scale accurate quantitative analysis of irisone synthesis related functional genes.

The invention relates to the technical field of genes, in particular to cloning and application of a spodoptera frugiperda sex pheromone precursor synthetase fatty acyl-CoA reductase SfruFAR1 gene. The research finds that the SfruFAR1 gene is specifically and highly expressed in the insect sex pheromone gland. Through heterologous expression in a yeast system and assisted by enzyme activity analysis and GC-MS identification, it is proved that the SfruFAR1 protein has the biological activity of catalyzing the reduction of multiple fatty acyl-CoA with different carbon chain lengths into corresponding fatty alcohol. Experimental results show that the SfruFAR1 protein plays a core role in a process of synthesizing a precursor of the lepidoptera insect sex pheromone and fatty alcohol which is a component of the precursor. According to the invention, a molecular foundation is laid for realizing in-vitro efficient biosynthesis of the lepidoptera insect sex pheromone by using the SfruFAR1 gene, and a key functional gene tool is provided.

The invention relates to the technical field of agriculture, in particular to an efficient ABE base editing tool based on Cas12i3 and application of the efficient ABE base editing tool. The Cas12i3-based efficient ABE base editing tool provided by the invention comprises a protein expression cassette and an RNA (Ribonucleic Acid) expression cassette, the protein expression cassette comprises a promoter, a 2 * BP NLS nuclear localization signal fragment, a 2 * adenine deaminase TadA8e, a connecting peptide, a Cas12i3 notch enzyme fragment and a terminator, the RNA expression cassette comprises a promoter, a crRNA sequence and a terminator; wherein the crRNA sequence is composed of a DR sequence and a target specific spacer sequence. The tool realizes efficient and specific single base substitution of A-to-G in a rice genome by utilizing the targeted binding characteristic of Cas12i3 and the efficient deamination activity of 2 * TadA8e, has the advantages of high editing efficiency, low off-target rate, wide application range and the like, provides an efficient and accurate technical means for plant functional genome research and molecular breeding, and has a wide application prospect. The creation of excellent rice varieties can be accelerated.

The application discloses a method for creating a fragrant glutinous millet by using a CRISPR / Cas12i.3 system and application thereof, and belongs to the technical field of genetic engineering. The application first applies a miniaturized CRISPR / Cas12i.3 gene editing system with independent intellectual property rights of China to the genetic improvement of millet, and establishes an efficient and precise gene editing technology system suitable for the crop. Compared with commonly used Cas9 and other systems, the Cas12i.3 protein has a smaller volume, is easier to construct a plant expression vector, significantly improves the transformation efficiency, and avoids foreign patent restrictions, thereby providing a key tool for functional gene research and molecular design breeding of millet.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The application discloses a corn plant height regulation gene combination and application thereof in creating multi-gradient plant height corn materials, and the gene combination comprises a gene ZmCPS1, a gene ZmbHLH156 and a gene ZmEMF1L1. The application uses a homozygous near-isogenic line carrying a single regulation plant height gene in a corn 9782 background BC4 as a basic material, and then uses two-by-two hybridization of different gene near-isogenic lines, combines KASP molecular marker assisted screening based on a functional gene, gene chip detection, conventional breeding technology and plant height phenotype analysis, and finally, 8 three-gene aggregation corn materials with gradient plant height are obtained. The application aggregates different genotypes of alleles, and has high practical application value in plant genetics and breeding work.

This invention discloses a method for wheat grain quality detection and functional gene analysis based on hyperspectral imaging technology, belonging to the field of agricultural testing technology. The method includes: extracting hyperspectral indices; measuring wheat grain quality indicators as manual measurements; assigning weight coefficients to each quality indicator to digitize the wheat quality phenotype; extracting characteristic wavelengths to establish predictive models for each indicator and wheat quality values; performing GWAS on hyperspectral indices and chemical indicators as phenotypic traits with genotypes to screen SNP loci, conduct co-location analysis, obtain candidate genes, and perform functional verification. This invention, through hyperspectral technology combined with chemometric modeling, is suitable for real-time monitoring during wheat quality breeding and processing; by combining spectral phenotype with GWAS gene mining, it overcomes the limitations of traditional quality detection that only serves phenotypic screening, forming a closed loop of "non-destructive detection - gene localization," providing targeted genes for wheat quality improvement.

The application discloses a storage medium, a groundwater biological stimulation remediation effect evaluation method, device and equipment, wherein the method comprises the following steps: collecting groundwater samples of a target area at multiple preset time points before biological stimulation agents are put; respectively determining monomer isotope values and molecular information of pollutants in the groundwater samples collected each time, and determining abundance data of functional genes and / or key enzymes; calculating the change range of specific indexes including the delta value of monomer isotope, the isotope value of nutrients or electron acceptors, and the abundance data of functional genes and / or key enzymes; and when the change range of the specific indexes is all rising, it is confirmed that the biological stimulation remediation is effective. The application improves the evaluation accuracy of the effectiveness of the biological stimulation method, and thus the remediation effect of the contaminated area to be remediated can be improved, and invalid cost investment can be avoided.

This invention belongs to the field of plant gene editing technology, specifically relating to a bifunctional gene editing system and its application in single-parent maize. The invention provides a bifunctional gene editing system comprising: a fusion protein expression cassette and a guide RNA combined expression cassette; wherein the guide RNA combined expression cassette includes: pegRNA associated with the maize D9 gene; and sgRNA associated with the maize YUC4 and YUC2 genes. Transient expression validation using the bifunctional gene editing system in maize protoplasts revealed that the system achieves a precise editing efficiency of 3.5% at the G571V site of the D9 gene, a knockout efficiency of 40%-65% for the YUC4 gene, and a knockout efficiency of 37%-54% for the YUC2 gene. This demonstrates that the technical solution of this application can simultaneously and efficiently achieve precise point mutation and gene knockout within the same cell, thus enabling its application in the synergistic improvement of lodging resistance in maize.

The invention discloses a sheep SNP (Single Nucleotide Polymorphism) molecular marker combination, a liquid chip and application of the sheep SNP molecular marker combination and the liquid chip. The sheep SNP molecular marker combination comprises 3, 192 SNP molecular markers, and the physical positions of the 3, 192 SNP molecular markers are determined by carrying out sequence alignment on the basis of a sheep reference genome AR-UIRambv3.0. According to the scheme, the SNP sites in the sheep SNP molecular marker combination are specific sites which are obtained by mining from re-sequencing data of large-scale sheep germplasm resources and can be used for distinguishing different sheep varieties, and the different sheep varieties can be effectively distinguished; meanwhile, the sheep SNP molecular marker combination also contains a large amount of functional genes and QTL-related SNP locus information, and can be used for gene identification and function analysis of important economic traits of sheep.

The invention belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to a gene for regulating and controlling the cold tolerance of rice in the seedling stage and application of the gene. In order to excavate the gene for regulating and controlling the cold resistance of the rice, the nucleotide sequence of the rice gene OsCYP75B3 is mutated by virtue of a CRISPR / Cas9 genome targeted modification technology, and an experimental result shows that the cold resistance of the mutated rice in the seedling stage is remarkably reduced, so that the gene OsCYP75B3 is proved to negatively regulate and control the cold resistance of the rice in the seedling stage; the functional gene for regulating and controlling the cold resistance of the rice in the seedling stage is determined for the first time, and an important reference is provided for analyzing a molecular mechanism of the cold resistance of the rice in the seedling stage; based on the discovery, it is expected that the expression level of the gene OsCYP75B3 is improved through an overexpression technology, the gene OsCYP75B3 is applied to rice genetic engineering breeding, the cold resistance of rice is finally enhanced, and a key technical support is provided for guaranteeing grain safety.