605results about How to "Quick screening" patented technology

The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.

System and method for providing a sample preparing arrangement (10) submergible in a liquid medium that includes a carrier structure (11) having at least one cavity (12) therein and that is in communication with an arrangement (13) for controllable generation of a magnetic field through influence of a control signal. The sample preparing arrangement includes a magnetic covering structure (14) for covering and / or uncovering the cavity in operative interaction with the magnetic field.

The invention provides a method for screening toxic substances in a sample by using solid phase support liquid-liquid extraction-GC MC. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, centrifuging an extracting solution after extraction, collecting a supernatant and removing the organic solvent in the supernatant, and preparing a sample liquid; adding the sample liquid into an activated solid phase support liquid-liquid extraction column, performing standing, performing eluting by using an eluate, and collecting the eluate; carrying out a chromatogram-mass spectrometry of the eluate. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 150 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

A protein obtainable from a non pathogenic microorganism, said protein having mucosa binding promoting activity and a molecular weight of 20-40 kD is disclosed. Application of such a protein or a peptide derived therefrom in a method of screening non pathogenic microorganisms for a microorganism capable of specifically binding mucosa, said method comprising detection in a manner known per se of the presence of a particular protein on or in a microorganism or in a culture of microorganisms, said particular protein being the already defined protein. Kits suitable for such a screening method are also disclosed. Use of a component selected from the group of components comprising a protein or peptide as defined an expression vector comprising nucleic acid encoding such protein or peptide a recombinant microorganism or a part of said microorganism expressing such protein or peptide, said part expressing mucosa binding promoting activity a non pathogenic microorganism capable of expressing such protein or peptide or a part of said microorganism, said part expressing mucosa binding promoting activity as pharmaceutically active component in a pharmaceutical composition for prophylaxis and / or treatment of disease or illness associated with a mucosa colonising pathogenic microorganism. Use of such components as food additive and compositions comprising such components are described.

A protein obtainable from a non pathgenic microorgansim, said protein having mucosa binding promoting activity and a molecular weight of 20-40 kD is disclosed. Application of such a protein or a peptide derived therefrom in a method of screening non pathogenic microorganisms for a microorganism capable of specifically binding mucosa, said method comprising detection in a manner known per se of the presence of a particular protein on or in a microorganism or in a culture of microorganisms, said particular protein being the already defined protein. Kits suitable for such a screening method are also disclosed. Use of a component selected from the group of components comprising a protein or peptide as defined; an expression vector comprising nucleic acid encoding such protein or peptide; a recombinant microorganism or a part of said microorganism expressing such protein or peptide, said part expressing mucosa binding promoting activity; a non pathogenic microorganism capable of expressing such protein or peptide or a part of said microorganism, said part expressing mucosa binding promoting activity as pharmaceutically active component in a pharmaceutical composition for prophylaxis and / or treatment of disease or illness associated with a mucosa colonizing pathogenic microorganism. Use of such components as food additive and compositions comprising such components are described.

The invention discloses SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof. 7 screened SNP sites, including SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7, are utilized to design 7 primer pairs of which the sequences are disclosed as SEQ ID NO:1-SEQ ID NO:14; and each primer pair can amplify genome sequences disclosed as SEQ ID NO:15-SEQ ID NO:21 of the mouse inbred line under the same conditions. The sequences amplified by each primer pair contain one specific SNP marker. The application of the SNP marker and primer pairs can achieve the goals of quickly performing primary screening and lowering the detection cost, thereby promoting the inbred line mouse genetic quality monitoring standardization progress.

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.

The invention relates to a comprehensive verification system and method for identities and documentary evidences, and belongs to comprehensive credential verification systems and methods. According to the comprehensive verification system, a verification system website is established, and the verification system website serves as a verification center; the verification system website is connected with a terminal of the verification system website through a network to form a verification network system. Individual registration information and unit registration information are included in the verification system website, and are the bottom layer basic information of the verification system website. According to the method, a registration method and a verification method are included, unique verification email addresses are automatically generated by using identity numbers and organizing institution codes and serve as verification bases, and simple, convenient and reliable verification is achieved. Wrong identity cards, repeat identity cards and fake identity cards can be conveniently and fast found and screened, and the problem of train ticket wiped by ticket touts can be solved; fake official seals and credentials can be identified; the problem of social insurance and birth certificate verification can be solved.

The invention provides a method and an apparatus for labeling face images. The method comprises: acquiring a face image set which corresponds to a specific personal name and comprises a plurality of face images, wherein each face image only comprises one face; establishing a whole object assumption that both random face images in the face image set belong to the same person, and verifying whether the whole object assumption is tenable by a predetermined face verification model; and if verifying the whole object assumption is tenable, labeling the plurality of face images in the face image set as a result that the face images in the face image set belong to the specific personal name. According to the scheme, a great number of images crawled from the Internet can be rapidly discriminated and screened, error images are efficiently removed, labor and time cost of labeling the face images is greatly saved, and a powerful guarantee is provided for a subsequent face identification model based on automatic labeling, which has high face image training accuracy; and meanwhile, according to the scheme, an existing data monopoly in the industry can be broken through, which is convenient for popularization of a face identification technology, thereby promoting the progress of the technology.

The invention aims to provide an establishment method and application of a mass spectrum database of a prohibited colorant in foods. The method provided by the invention comprises the steps of: (1) establishing a first-level mass spectrum database and a second-level mass spectrum database of the colorants; and (2) separating the colorants with similar properties in the established mass spectrum databases. According to the establishment method and the application, the technical evaluation foundations are provided for the law enforcement; a purpose of fast screening the unknown prohibited added colorants is achieved by virtue of the mass spectrum databases; and the establishment method and the application can be helpful for strengthening the food detection of markets and carrying out preventive measures to effectively protect life safety of people.

A tissue chip for detecting autoantibody is composed of substrate and the matrix on the substrate. Said matrix may be paraffin wax coated tissue, frozen fresh tissue, or cell specimen. An indirect immunofluorescence technique is used to detect, screen and analyze a great deal of autoantibodies in the blood serum of autoimmunopathy patient.

The invention discloses a GC-MS non-target method used for rapid screening of plant differential metabolites, and belongs to the technical field of flavours and fragrances, and metabonomics study technology. According to the GC-MS non-target method, modern GC-MS full scanning technology is adopted to obtain chemical ingredient information of volatile substances in plant; multidimensional Gaussian smoothing is adopted to extract substance information in a TIC picture; dynamic programming algorithm is adopted to calibrate chromatographic peak time shifting among samples, realize aligning of chromatographic peaks belongs to one same substance, achieve comparability of substance information among different samples; nearest neighbor-clustering algorithm is adopted to realize compound registration; and at last variance analysis is adopted to screen compounds with significant difference from different groups. According to the GC-MS non-target method, compound mass spectrum information is output so as to obtain a MSP file; and the MSP file can be input into the NIST database to search compounds. The GC-MS non-target method can be used for locking compounds with dissimilarities in plant samples rapidly, and is suitable for rapid analysis determination of a large amount of samples.

The invention relates to an adeno-associated virus (AAV) producer cell comprising nucleic acid sequences encoding: rep / cap gene; helper virus genes; and the DNA genome of the AAV vector particle, wherein the nucleic acid sequences are all integrated together at a single locus within the AAV producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that the nucleic acid vectors comprise nucleic acid sequences encoding: rep / cap gene, and helper virus genes. The invention also relates to uses and methods using the nucleic acid vectors in order to produce stable AAV packaging and producer cell lines.

The invention discloses a design way of primers for in-vitro qualitative detection of newborn inherited metabolic disease genes. The design way comprises the following steps: (1) acquiring relevant disease-causing gene information of inherited diseases: acquiring the relevant disease-causing gene information, including gene location information and mutation site information of a plurality of inherited diseases through an OMIM (Online Mendelian Inheritance in Man) database, and acquiring exon and intron information of relevant genes from an NCBI (National Center for Biotechnology Information) database according to the obtained gene information; (2) designing primers specific to the relevant genes: designing corresponding specific primers F1 and R1 specific to an exon and an exon-intron combination area of each gene. The invention also discloses the first-round PCR (Polymerase Chain Reaction) primer sequence list of syndromic deafness, a first-round PCR primer list of G6PD genes, and a first-round PCR primer sequence list of SLC12A3 genes. The primers can be used for detecting relevant genes of 22 types of common newborn inherited diseases.

An apparatus, module, methods and systems for automated, standardized assessment and analysis of a human olfactory system's odor detection ability as an indicator or predictor of cognitive impairment or change in cognitive health, and other health conditions such as diabetes. Notably, the present invention is operable for use across all age groups of humans and provides quantitative detection and analysis of a human olfactory system's detection ability compared to a relevant demographic population.

The invention discloses a primer, probe and kit for detecting type-16 HPV (human papillomavirus). The primer and the probe are suitable for screening the HPV related diseases quickly, accurately and simply, do not cross-react with other viruses, are capable of detecting common high and low risk types of HPVs, two types of HPVs and 14 types of HPVs in total, cover a wide range of high risk types of HPVs and are more accurate and complete in detection than kits in the present market. The kit further comprises negative, positive and weakly positive quality controls and helps further increase detection accurateness.

The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.

The invention discloses a general nucleic acid isothermal detection reagent of respiratory tract infection associated adenoviruses. The invention provides recombinase polymerase nucleic acid detection set nucleic acid which is used for identifying respiratory tract infection adenoviruses and is composed of a primer pair and a probe; the primer pair is composed of a single-stranded DNA (Deoxyribonucleic Acid) molecule represented by a sequence 1 in a sequence table and a single-stranded DNA (Deoxyribonucleic Acid) molecule represented by a sequence 2 in the sequence table; the probe is composed of A-dt-FAM-tetrahydrofuran-dt-BHQ1-B, wherein the nucleotide sequence of A is a sequence 3; the nucleotide sequence of B is a sequence 4. An experiment testifies that general RPA (Recombinase Polymerase Assay) detection primer and probe are designed aiming at six types of the respiratory tract infection associated adenoviruses; an RPA detection system and method for the respiratory tract infection associated adenoviruses are established; the technology can be used for carrying out field rapid screening and detection on the respiratory tract infection associated adenoviruses, has an important value and can realize rapid screening of unknown samples.

The invention provides a rapid detection method for water-soluble azo dyes in foods. The method comprises the steps of (A), sample extraction; (B), polyamide column concentration and purification; (C), elution of pigments; and (D) qualitative detection. According to the rapid detection method, the rapid screening can be timely and effectively carried out on suspected samples, the method is independent of large-scale instruments and equipments, and lawbreakers can be harshly attacked within the shortest time; and besides, the rapid detection method further has the advantages of short detection time, wide applicable range, high detection accuracy, low detection limit, strong anti-interference capacity and the like.

The invention relates to a detection method for redox materials in food, which is used for detecting the quantitative concentration of the residue redox materials in food in a short time. The method is implemented by a detecting test piece and a detector, and comprises the following steps: pretreating a proper amount of a food test sample, preparing a certain amount of the preteated food test sample, and dissolving the pretreated food test sample in buffer solution; subjecting the buffer solution containing materials to be detected and the formula layer of the detecting test piece to a redox reaction; the detector provides a reaction level to the reaction electrode of the test piece to acquire an electrical signal; and the content of the materials to be detected in the food test sample can be acquired by the electrical signal. The invention also provides the detecting test piece and the detector, which are applicable to the detection method.

The invention provides a method for screening toxic substances in a sample by using solid phase microextraction-GC-MS. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, and producing an extract; centrifuging the extract, collecting a supernatant and removing organic solvents in the supernatant, performing centrifuging again, collecting a supernatant, and producing a sample liquid; inserting a PDMS extraction head into the sample liquid for carrying out solid phase microextraction, after extraction, fetching out the PDMS extraction head, inserting the extraction head into the GC-MS for desorption, and analyzing the desorbed sample which enters into a chromatographic column by using a GC-MS method. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 60 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

The invention relates to a pilot protection method for a power transmission line based on signal distance and Bergeron model, belonging to the technical field of relay protection for electric power systems. The pilot protection method comprises the following steps of: when the power transmission line has faults, in a short-time window, measuring voltages (uM, uN) and currents (iM, iN) at the head end (M) point and the tail end (N) point of the power transmission line, simulating and calculating the current at the tail end of the power transmission line by using the actually measured voltage (uM) and current (iM) at the head end according to the line current distribution rule expression of the Bergeron model, comparing the waveforms of the quasi tail end current and the actually measured tail end current (iN), calculating the mutual distance degree between the quasi current and the actually measured current (iN), comparing the calculated mutual distance degree with the set mutual distance degree setting value, and identifying faults inside or outside the region according to the size relationship of the two mutual distance degrees. The pilot protection method is not influenced by excesssive resistance and fault initiating angle and has the advantages of quickly and reliably identifying faults inside and outside the region and the like.

The invention relates to a method for cross-frequency-band power line communication, in particular to a self-learning method for a cross-frequency-band power line communication frequency. The frequency range where the method is applied is 150kHz-12MHz. The method comprises the following steps that (1) a default working frequency set is determined; (2) a leader sequence is adopted to conduct pre-selection on the default working frequency set, default working frequencies where the leader sequence cannot be correctly detected are screened out, and the default working frequencies where the leader sequence is correctly detected are used as potential working efficiency; (3) on the potential working efficiency, control packages are transmitted between a master station and a slave station, and the best working frequency is determined. According to the method, the best frequency (a center frequency point and bandwidth) can be independently learned within the wide spectral range, so that the frequency and data rate are adapted independently according to actual channel conditions. Therefore, effectiveness is improved, the coverage area is expanded, and human intervention is reduced.

The invention relates to a method for quickly screening benzedrine drugs and morphine drugs in hair on the spot. The method sequentially comprises the following steps of step 1, placing the hair to be detected, and grinding media together in a grinding tube of a crusher, and performing grinding and pulverizing treatment for the first time in the grinding tube of the crusher; step 2, after the grinding is performed for the first time, adding a solvent to the grinding tube, dissolving the hair in the grinding tube, and performing grinding and pulverizing treatment for the second time in the grinding tube of the crusher; and step 3, taking liquid in the grinding tube after the grinding and pulverizing treatment is performed for the second time, performing detection on a corresponding colloidal gold plate, and displaying a screening result. Through the adoption of the method disclosed by the invention, the simple, convenient, quick and on-the-spot operation of screening the drugs in the hair can be realized; the method is high in specificity, high in selectivity, and high in sensitivity, and can be applied to screening the hair of actual drug addicts, and can be applied to the field of forensic toxicological analysis, and the screening and solving of relevant cases.

The present invention relates to a combination comprising a plurality of cDNAs which are differentially expressed in treated rat liver and in human liver and which may be used in their entirety or in part to diagnose, stage, or treat a liver disorder, to monitor diagnostic and therapeutic applications, to detect metabolic and toxicological responses, and to elucidate drug mechanism of action.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention discloses a novel fault attack method aiming at the Advanced Encryption Standard (AES-128) algorithm, which comprises the steps that: firstly, an attacker randomly selects a plaintext, acquires the correct ciphertext of the plaintext under the action of an initial secret key; and then the attacker encrypts the randomly selected plaintext, optionally selects any one of first three columns of sub-secrete keys at the ninth round in an encryption operation progress, performs multi-byte random fault induction to the one column of sub-secret keys, and acquires a wrong ciphertext comprising random faults; next, the attacker performs multi-byte random fault induction to the rest three columns of sub-secret keys at the ninth round through the same operation till the rest three columns of sub-secret keys are recovered completely and sub-secrete keys at the tenth round are acquired; at last, the initial secret key is acquired through reverse calculation. The novel fault attack method aiming at the AES-12 algorithm disclosed by the invention attacks the sub-secret keys at the ninth round and operates any column of the needed sub-secret keys without affecting operation results of each other, therefore, an AES-128 password system can be treated by differential fault attack by a plurality of equipments at the same time, thus acquiring initial secret key information rapidly and saving more time.

The invention discloses nucleic acid for detecting Zika virus, a real-time fluorescence RPA kit and a detection method thereof. The real-time fluorescence RPA kit is convenient to use, the quantity of reagents adopted is small, the cost is low, the compatibility of required instruments is high, reaction can be performed on a real-time fluorescence PCR instrument and instruments with a fluorescent trapping function, and the greatest advantage of the kit lines in performing isothermal amplication and being capable of detecting fluorescence signals in real time within 10 to 30 min. The detection method greatly simplifies the operation process, reduces steps of repetitive operation, saves time, reduces labor force consumed by repetitive operation, and effectively lowers the cost, and a detection result shows that the method is high in specificity and sensitivity, and rapid and accurate screening of Zika virus is realized.

The invention provides a method for detecting and disposing an abnormal traffic attack, comprising the following steps: 1) filtering known abnormal traffic and normal traffic of a visitor IP address; 2) according to a current traffic numerical value of the traffic passing the filtration of the visitor IP address and a corresponding traffic template numerical value in a TOPN dynamic filtering table, distinguishing the abnormal traffic and the normal traffic passing the filtration in the visitor IP address; 3) discarding the abnormal traffic, and forwarding the normal traffic. The invention also provides a device for detecting and disposing the abnormal traffic attack, comprising a traffic receiving unit, a static filtering unit, a dynamic detection filtering unit and a self-learning unit connected orderly, wherein the dynamic detection filtering unit is connected with an operation and deciding unit and the self-learning unit. The method and the device provided by the invention can improve disposal efficiency and detection accuracy for abnormal behaviors, and can quickly detect and dispose the abnormal attack traffic without influencing normal traffic access.