224results about How to "Quick screening" patented technology

The invention relates to an underground pipe network leakage position monitoring method and device, a computer program product and electronic equipment, and belongs to the field of remote sensing monitoring. According to the method, the underground pipe network leakage position in a large-area range of a city is detected by utilizing a remote sensing means. The monitoring method provided by the invention comprises the following steps: determining a bare soil backscattering coefficient of a to-be-monitored area; inputting the bare soil backscattering coefficient into a target soil moisture identification model, and determining the soil mass moisture content of the to-be-monitored area through the target soil moisture identification model; and comparing the soil mass water content of the to-be-monitored area with the leakage soil mass water content threshold value of the to-be-monitored area, and determining the leakage point of the pipeline in the to-be-monitored area. The monitoring method provided by the invention is low in cost, low in manufacturing cost and small in loss, and has the advantages of rapid, lossless and automatic detection of the leakage position point of the pipe network in a large-area range.

The utility model relates to the field of electrostatic spraying machines, in particular to a powder filtering and screening device which comprises a supporting mechanism, a screening mechanism used for shaking powder up and down and uniformly vibrating the powder and a sliding mechanism used for driving the screening mechanism to slide on the two sides, and a driving structure is arranged in the middle of the supporting mechanism. The screening frame is driven to move up and down through the connecting frame, meanwhile, vibration is transmitted to the screening frame through the vibration motor, powder can be rapidly spread and screened immediately, the cracking rods are turned over and reset to a certain degree through the inclined screening frame, and screened blocky powder is knocked and smashed immediately. And the broken powder is sieved and separated again, so that the waste of the powder is reduced.

The invention discloses a screening method of fentanyl substances, and belongs to the technical field of detection and identification of fentanyl substances. Comprising the following steps: S1, acquiring m types of fentanyl and fentanyl substance samples, and cracking to obtain characteristic warning fragments; s2, establishing a feature warning fragment database; s3, the warning ion database is imported into TraceFinder software, after a sample to be screened is split, fragments and characteristic warning fragments are deduced through the TraceFinder software according to the fragmentation law and the characteristic theory of each type of fentanyl, and whether the fragments and the characteristic warning fragments are fentanyl substances or not is judged. According to the method, n characteristic warning fragments of fentanyl are obtained after high-resolution mass spectrum pyrolysis characteristics of m known typical fentanyl compounds are obtained, and the characteristic warning fragments are used for constructing a database, so that fentanyl substances can be quickly screened and identified.

The invention relates to the technical field of medicine detection, and discloses medicine raw material detection equipment for improving the purity of raw materials, the medicine raw material detection equipment comprises a base and a supporting box, the bottom of the supporting box is fixedly connected to the outer side of the base, the top of the base is fixedly connected with a detection mechanism, and the top of the detection mechanism is fixedly connected with a detection plate; the top end of the supporting box is fixedly connected with a driving box, multiple filtering and impurity removing can be conducted on medicine raw materials through the impurity removing assembly, impurities in the raw materials can be screened out while the caked raw materials are screened out, the purity of the medicine raw materials is improved, the content of invalid or harmful ingredients in medicine can be reduced by removing the impurities, and therefore the medicine raw materials can be conveniently taken out. The purity and quality of the medicine are improved, the raw materials with higher purity can be used for preparing the medicine which is more efficient, safer and more stable, the treatment effect and the satisfaction degree of a patient are improved, and the content of active ingredients of the medicine can be improved by removing impurities, so that the curative effect of the medicine is enhanced.

The invention discloses a light impact-resistant battery protection shell quality detection device, and particularly relates to the technical field of battery protection shells. The light impact-resistant battery protection shell quality detection device comprises a rack, a second motor is arranged on the outer wall of the rack, a first bevel gear is fixedly connected to the outer wall of a first transmission rod, and a connecting rod is fixedly connected to the inner wall of the second bevel gear; the outer wall of the first threaded rod is in threaded connection with a threaded sleeve, and the outer wall of the first bevel gear is in engaged connection with a third bevel gear. According to the quality detection device for the light impact-resistant battery protection shell, through the arrangement of a first bevel gear, a threaded sleeve, a driver and a protection shell, a worker can conveniently and synchronously detect extrusion and mechanical impact of the battery protection shell, and the efficiency of detecting the quality of the battery protection shell by the worker is improved; and meanwhile, the deviation of the battery protection shell during quality detection is prevented, so that the accuracy of the quality detection of the battery protection shell by the detection device is guaranteed.

The invention discloses a corn seed fine selection device and method, and belongs to the technical field of screening equipment, the corn seed fine selection device comprises a supporting frame, a swing seat, a screen mesh assembly and an adjusting assembly, the supporting frame is located above the swing seat, and the screen mesh assembly is located in the supporting frame; the screen assembly sequentially comprises a first screen tray, a second screen tray, a third screen tray and a bottom tray from top to bottom, the first screen tray, the second screen tray and the third screen tray are connected in an inserted mode to form screen holes with different hole diameters, and rotating shafts and discharging pipes are arranged on the left sides and the right sides of the first screen tray, the second screen tray, the third screen tray and the bottom tray correspondingly. The second sieve tray, the third sieve tray and the base plate are clamped by an upper group of adjusting nuts and a lower group of adjusting nuts and then move up and down along the direction of the screw rod; and the second sieve tray and the third sieve tray are turned over through the rotating shaft and the discharging pipe. According to different varieties of corn seeds, the screen mesh assemblies are combined to form corresponding screen holes, the screening efficiency is improved, and meanwhile the screen mesh assemblies can be adjusted to form multi-stage screening of multiple screen trays.

The gravel vibrating screen for road construction comprises a lower end support, connecting springs are evenly fixed to the upper surface of the lower end support, a supporting ring is installed between the upper ends of the connecting springs, and a cylindrical screening shell is arranged on the upper surface of the supporting ring. The upper surface of the supporting ring is connected with a rotating screening part through a rotary support, the outer side of the rotating screening part is attached to the inner side of the cylindrical screening shell, a discharging port is formed in the lower end of one side of the cylindrical screening shell, a material scattering part is arranged at the upper end of the interior of the cylindrical screening shell, and a rotating driving part for driving the rotating screening part to rotate is arranged on the upper surface of the material scattering part. The gravel vibrating screen for road construction is simple in structure and easy and convenient to operate, vibration screening can be conducted, the gravel vibrating screen can be driven to rotate during vibration screening, the screening effect is better, and screening is faster.

The invention belongs to the technical field of nano material application and chemical detection, and particularly discloses a method and a kit for detecting the content of D-amino acid based on a Pt / Ti3C2Tx nano material and application of the method and the kit. The method comprises the following steps: incubating a sample to be detected and D-amino acid oxidase together; after the incubation is completed, adding a Pt / Ti < 3 > C < 2 > T < x > nano material and a chromogenic substrate TMB (Tetramethylbenzidine), and reacting under laser irradiation; and after the reaction is finished, determining the content of D-amino acid by a colorimetric detection method. The kit comprises D-amino acid oxidase, a Pt / Ti < 3 > C < 2 > T < x > nanometer material and TMB (Tetramethylbenzidine). The Pt / Ti < 3 > C < 2 > T < x > nanometer material has local surface plasma resonance effect and peroxidase-like catalytic activity, a colorimetric detection platform constructed based on the Pt / Ti < 3 > C < 2 > T < x > nanometer material is applied to clinical saliva sample detection, has the characteristics of high sensitivity, high selectivity and the like, and can provide a rapid, simple and painless new path for early screening of gastric cancer.

The invention discloses screening equipment based on computer visual identification, and relates to the technical field of visual detection screening, and the technical scheme is that the screening equipment comprises a conveyor belt, packages are conveyed on the conveyor belt, an identification shell is fixedly connected to the upper part of the conveyor belt, adjusting mechanisms are symmetrically arranged in the identification shell, and identification cameras are fixedly connected to the upper parts of the adjusting mechanisms; according to the invention, the parcels are transported above the conveyor belt and enter the identification shell, then the parcels are firstly detected by the identification camera arranged on the adjusting mechanism, and the upper parts and the two sides of the parcels can be identified through the identification camera, so that whether the parcels are damaged or not is checked; and the recognition angle of the recognition camera can be adjusted through the adjusting mechanism, so that the recognition effect of the recognition camera is improved, the package is driven to rotate through the turnover mechanism, the bottom of the package is turned out, and the situation that products with the damaged bottoms of the packages are mixed with qualified products is effectively avoided.

The invention discloses gradient utilization and recovery equipment for new energy automobile batteries, relates to the field of battery recovery, and solves the problem that existing recovery equipment is difficult to normally convey solid disintegrating slag, the gradient utilization and recovery equipment comprises an equipment shell and a feeding cylinder fixedly mounted at the top of the equipment shell, and a crushing wheel set is mounted on the inner side of the feeding cylinder; a first storage box is fixedly installed on the inner wall of the bottom of the equipment shell, a second storage box is fixedly installed on the left side of the first storage box, and a water injection valve extending to the outer side of the equipment shell is fixedly installed on the outer side of the second storage box. The material screening mechanism is used for screening liquid raw materials and solid raw materials in the equipment shell, and the material screening mechanism is mounted on the inner side of the equipment shell; through the screening mechanism, the crushed battery raw materials can be received in batches, a solid material and a liquid material in the battery raw materials are separated in a rotary conveying mode, the solid raw material and the liquid raw material in the battery raw materials can be screened out conveniently, and therefore the rapid screening effect is achieved.

The invention provides a non-targeted screening analysis method for veterinary drug residues in an animal-derived product, and belongs to the technical field of food safety detection.The method comprises the following steps that 1, after the animal-derived product is pulped, pretreatment is conducted, and sample solutions with different concentrations are obtained; (2) detecting the sample solutions with different concentrations by using an HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method to obtain original data of samples with different concentrations; 3) performing metabonomics analysis on the original data by using analysis software, and screening variable projection importance VIPgt; all variables of 1; and 4) utilizing a support vector machine recursive feature elimination method SVMRFE to assist metabonomics analysis, and finally determining feature variables representing known veterinary drugs. Compared with simple metabonomics analysis, the method provided by the invention has the advantages that the screening precision is higher, and veterinary drug residues in animal-derived products can be comprehensively, accurately and quickly screened.

The invention relates to the technical field of metering wiring of a three-phase four-wire electric energy meter, in particular to a method for quickly screening wrong wiring of a three-phase four-wire electric energy meter, which is mainly used for quickly and accurately screening the three-phase four-wire electric energy meter with wrong wiring on the basis of data acquisition of a power consumer electricity utilization information acquisition system. Comprising the following steps: step 1, analyzing voltage and voltage and current phase angle characteristics of a three-phase four-wire electric energy meter, and analyzing a phase angle with normal voltage and a phase angle with normal voltage and current; 2, acquiring the voltage of the three-phase electric energy meter and the phase angle of the voltage and the current in real time through a power consumer electricity utilization information acquisition system; 3, analyzing and screening out users with abnormal voltage and current angles; and 4, exporting a wrong wiring user list, and performing manual on-site verification and rectification.

The invention discloses a children autism risk assessment method and system based on multi-modal collaborative reasoning and a vertical domain large model, and the method comprises the steps: collecting the audio data of a to-be-assessed child and a parent in a parent-child interaction link, and collecting the video data through collection equipment; analyzing and marking the collected audio data, and generating a sounding record and a transcription text with a timestamp; performing real-time analysis and labeling on the collected video data, and generating sight line, expression and action recognition records with timestamps; performing independent analysis on the preprocessed multi-modal data, performing feature fusion and collaborative analysis on the multi-modal data such as audio, text and video by using a multi-modal collaborative reasoning method, and generating a scientific data index for autism risk assessment; training a vertical domain large model special for autism risk assessment; and inputting the generated data indexes into a vertical domain large model, and outputting targeted assessment suggestions by the large model in combination with daily information of the children to be assessed obtained by interviews and conversations with parents.

The screening device comprises a U-shaped base, a first screening plate, a second screening plate and a third screening plate are arranged in the U-shaped base, rectangular annular baffles are fixedly connected to the upper ends of the first screening plate, the second screening plate and the third screening plate, and a swing mechanism is fixedly connected to the side wall of the front end of the U-shaped base. And the swing mechanism is associated with the first sieve plate, the second sieve plate and the third sieve plate. The swinging mechanism is matched with the first sieve plate, the second sieve plate and the third sieve plate of which the sieve holes are sequentially reduced from top to bottom to perform swinging screening on antibacterial amino molding plastic particles, so that small particles or incomplete particles can be conveniently and quickly screened out, the structure is simple and practical, and the material blocking mechanism is matched with the material guide chute, so that the screening efficiency is improved. Bacteriostatic amino molding plastic particles detained on the first sieve plate, the second sieve plate and the third sieve plate can be conveniently and rapidly discharged, small particles or incomplete particles obtained after screening are collected through the uncovered recovery tank, excess materials can be conveniently recycled, and energy conservation and consumption reduction are achieved.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

The utility model relates to the technical field of dropping pill packaging, in particular to wine propolis dropping pill automatic packaging equipment which comprises a fixing frame, a first supporting frame and a second supporting frame are arranged on the top face of the fixing frame, and a classification assembly used for screening propolis dropping pills is arranged on the top face of the fixing frame. A conveying assembly used for recycling propolis dropping pills is arranged in the fixing frame, a packaging assembly used for packaging the propolis dropping pills is arranged on the top face of the second supporting frame, and a preset gap between a first material guide rod and a second material guide rod is gradually increased in the inclined direction, so that the wine-made propolis dropping pills fall off at different positions in the falling and rolling process. The dropping pills falling on the packaging assembly are packaged, and the dropping pills falling on the conveying assembly are conveyed and recycled by the conveying assembly, so that the structure can quickly screen and package the wine-processed propolis dropping pills, and the packaging quality of the wine-processed propolis dropping pills is improved.

The utility model relates to the technical field of polishing material processing, and discloses a high-precision glass polishing material granularity screening machine which comprises a supporting base, three inserting grooves distributed at equal intervals are formed in one side of the supporting base, collecting drawers are fixedly inserted into the inserting grooves, and a discharging box is fixedly connected to the other side of the supporting base. Spring seats are fixedly connected to the four corners of the upper side face of the supporting base correspondingly, springs are fixedly connected to the four spring seats correspondingly, a screening machine is fixedly connected to the upper ends of the springs, an assembly cover covers the top of the screening machine, a connecting block is fixedly connected to the bottom of the screening machine, and two vibration motors are fixedly connected to the side, away from the screening machine, of the connecting block. The glass polishing material screening machine further comprises a screening device which is arranged in the inner cavity of the screening machine, efficient and accurate screening of glass polishing materials is achieved through the screening device which is convenient to disassemble, the screening efficiency and precision are improved, a screen can be rapidly replaced, the maintenance difficulty and cost are reduced, and meanwhile cleaning and repeated use are convenient.

The utility model discloses a sand screening device for building construction, and relates to the technical field of sand screening for building construction, the sand screening device comprises a device main body and a sand screening mechanism, the sand screening mechanism is arranged on the inner side of the device main body, and by arranging the sand screening mechanism, the telescopic length of a hydraulic lifting rod is adjusted, so that the position of a sand throwing plate is conveniently changed according to the sand amount; sand in the sand storage tank is thrown to the sand screening net through the sand throwing plate, the sand is more dispersed and uniform in the falling process through throwing, when the sand is thrown up through the sand throwing plate, the mass stress of large-particle broken stones mixed in the sand is larger, and therefore the large-particle broken stones are thrown farther than the sand, the large-particle broken stones fall into the collecting bin, and the collecting efficiency is improved. And meanwhile, the sand blocking fan blows air inwards, so that the sand is subjected to outward wind resistance, a large amount of sand is prevented from being thrown into the collecting bin, the sand screening effect is ensured, broken stones entering the collecting bin are screened through the sand filtering screen, and normal sand entering the collecting bin can fall into the sand outlet so as to be taken and used together.

The utility model discloses reciprocating type screening equipment for constructional engineering. The reciprocating type screening equipment comprises a reciprocating mechanism and a first driving motor installed on one side of the reciprocating mechanism. A conveying mechanism, a second driving motor and a third driving motor are arranged on one side of the reciprocating mechanism, and the second driving motor and the third driving motor are installed at the bottom of the conveying mechanism. The reciprocating mechanism comprises a support, one side of the support is fixedly connected with a first supporting block, and one side of the bottom of the first supporting block is fixedly connected with a first driving motor; the output end of the first driving motor penetrates through the first supporting block and is fixedly connected with a first rotating disc, and the outer side of the first rotating disc is connected with a belt in an attached mode. A second rotating disc is connected to the end, away from the first rotating disc, of the belt in an attached mode, a connecting rod is rotationally connected to one side of the second rotating disc, gravel of different sizes can be rapidly screened by arranging a reciprocating mechanism, and gravel feeding can be rapidly and evenly conducted on the device by arranging a conveying mechanism.

The utility model relates to the technical field of straw cutting-off, in particular to an agricultural harvesting straw cutting-off machine which comprises a cutting-off box. The device further comprises a hole plate and a crushing roller, a cutting-off box is fixedly connected, a cutting-off roller is rotationally connected into the cutting-off box, and a long blade is fixedly connected to the outer side of the cutting-off roller through a fixing block. Through the use of the long blade and the crushing roller, the controller controls the cutting air cylinder to drive the cutter to primarily cut moving straw, the cutting length can be flexibly adjusted, the single cutting load is reduced, the abrasion to the cutter is reduced, the primarily cut straw falls into the cutting box, the cutting roller rotates to drive the long blade to rotate, and the straw can be cut into the straw. The two crushing rollers rotate relatively, the straw is uniformly crushed into fine particles, the overall treatment efficiency of the straw is improved through three-stage cutting, the diversified market requirements are met, and the production efficiency is improved.

The invention discloses a land restoration device for land reclamation in coastal areas, relates to the field of land restoration, and solves the problem that the existing land restoration device is prone to causing soil to fall when digging soil. The device comprises a device shell and two mounting seats symmetrically mounted on the bottom of the device shell, a rotating rod 1 is rotatably mounted between the two mounting seats, both ends of the rotating rod 1 are fixedly connected to rotating wheels, a motor is fixedly mounted on the left inner wall of the device shell, a rotating rod 2 is fixedly connected to the output end of the motor, a plurality of equally spaced transmission belts 1 are arranged on the outer sides of the rotating rod 1 and the rotating rod 2, a plurality of equally spaced digging claws are fixedly connected to the outer side of the transmission belt 1, a soil taking mechanism is installed inside the device shell, and the invention uses the soil taking mechanism to make the digging claws on the transmission belt 1 dig soil and take materials in turn, and then take out the soil on the digging claws, thereby achieving the effect of automatic soil taking.

The invention relates to the technical field of antibody detection, and discloses a canine entomophilous disease quintuplet antibody detection card, a preparation method thereof and a kit. The detection card comprises the following five detection antigens a)-e): a) a leishmania antigen which is fusion protein of rk39, rk26 and rk9; b) a babesia antigen which is a fusion protein of TRAP and rBcMSA1 / rBcSA1; c) an amorphous antigen which is a fusion protein of P44 and OMP-1X; the detection card and the kit have the advantages that joint detection of IgG (immunoglobulin G) and IgM (immunoglobulin M) antibodies corresponding to canine Ehrlichia, canine anaplasmosis, canine babesiosis, canine lyme disease and canine leishmania can be realized by the detection card and the kit, and the detection sensitivity and the specificity are high. The detection card and the kit can be used for detecting canine Ehrlichia, canine anaplasmosis, canine babesiosis, canine lyme disease and canine leishmania. The detection card and the kit have the advantages that the detection card and the kit can be used for detecting canine Ehrlichia, canine anaplasmosis, canine babesiosis and canine leishmania;

This invention discloses a stator assembly magnetic tile anti-tipping mechanism, belonging to the field of motor assembly technology. It includes a frame, and further comprises: an adhesive application mechanism, a magnetic tile assembly mechanism, a magnetic tile clamp installation mechanism, and a feeding mechanism. The leftmost side of the frame is equipped with an adhesive application mechanism for applying adhesive to the inner wall of the motor housing. The middle left side of the frame is equipped with a magnetic tile assembly mechanism for inserting the magnetic tiles into the motor housing. The middle right side of the frame is equipped with two magnetic tile clamp installation mechanisms for inserting magnetic tile clamps into the motor housing. The rightmost side of the frame is equipped with a feeding mechanism for inspecting and unloading the assembled product. The magnetic tile assembly mechanism includes: a magnetic tile pressing component, a feeding component, and a magnetic tile anti-tipping component. The magnetic tile pressing component is installed on the middle left side of the frame, and the feeding component is installed on the front side of the frame. Through this method, the invention can prevent the magnetic tiles from tipping over during the installation of the motor housing, while also enabling the recycling of the positioning core assembly.

The utility model discloses a drum-type seed screening device, which relates to the field of seed screening, and comprises a box body and a multi-section screening drum, a front-section drum and a rear-section drum are fixed on two side box walls of the box body in an inserting manner, two ends of the multi-section screening drum are respectively and rotatably mounted on the front-section drum and the rear-section drum through a front-end bearing and a rear-end bearing, and the front-section drum and the rear-section drum are fixed on the front-section drum and the rear-section drum. A motor is fixedly installed on the inner side wall of the box body through a motor frame, the motor is connected with a long rotating shaft, the long rotating shaft is fixedly sleeved with a driving gear, the driving gear is in meshed connection with a barrel gear, the barrel gear is fixedly arranged on the multiple sections of screening barrels in a sleeving mode, and a swing seat is fixedly installed at the top of the inner side of the box body; a swing rod is rotatably mounted at the bottom of the swing seat, and a beating ball is fixedly mounted at the bottom end of the swing rod. The seed screening device has the beneficial effects that rapid screening of seeds is achieved through the multiple sections of rollers, meanwhile, blockages stuffed into screening holes are vibrated off in real time through beating, blockage is prevented, and disassembly and cleaning are not needed.

The invention relates to an indirect ELISA (enzyme-linked immuno sorbent assay) detection kit for a sheep bacteroides nodules antibody and application thereof, belonging to the technical field of biological detection. In order to solve the technical problems that a bacterium separation identification and PCR (Polymerase Chain Reaction) detection method of bacteroides nodulosus has defects and a serological identification method for bacteroides nodulosus does not exist in the prior art, the invention adopts a sheep bacteroides nodulosus outer membrane protein Omp1D as a coating antigen in an indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit; rapid detection and screening of bovine and sheep bacteroides nodules infection can be realized, and the kit has the characteristics of simple operation, rapidness and sensitivity, accurate result, good specificity and repeatability and the like; meanwhile, the indirect ELISA detection kit for the sheep bacteroides nodules antibody has the characteristics of being simple in preparation method, low in cost, suitable for large-scale production and the like, a rapid, simple and sensitive diagnostic kit for detecting bacteroides nodules infection is provided for large-scale breeding of animal husbandry, and a technical support is provided for preventing and controlling bacteroides nodules infection.

The application discloses a domain name resolution method and device based on domain name request risk management and control, the method comprises the following steps: identifying domain name requests as normal domain name requests or abnormal domain name requests; according to the user domain name access behavior data set and the abnormal domain name association strength, evaluating the security rating and the security score of the user; sending the normal domain name requests initiated by users with different security ratings to the DNS server subset cluster corresponding to their levels, so that the DNS server subset cluster of the level responds to the normal domain name requests in order based on the security score; predicting the normal domain names with high probability of access to form a domain name resource list, which is used to cache the domain name resource list to the selected DNS server when the security rating of the user is higher than the set threshold, so as to improve the domain name resolution cache hit rate and the resolution efficiency. The application solves the problem of reduced performance of the domain name server caused by the low utilization rate of the conventional DNS server due to the zombie network attack.

The present invention provides a one-step multiplex fluorescence quantitative PCR kit for detecting and distinguishing poxviruses. It comprises a quantitative PCR reaction solution, an enzyme mixture, a primer-probe mixture, a standard, a positive control substance, and a negative control substance. The poxviruses include monkeypox virus, cowpox virus, and varicella virus. The present invention designs highly specific primer probes according to the highly conserved nucleotide sequences of the three poxviruses and the internal standard RNase P, and simultaneously detects and distinguishes the presence of monkeypox virus, cowpox virus, and varicella virus from samples such as blood, follicle fluid, and rash swabs through a PCR reaction, and uses RNase P as the internal standard for evaluating the amplification system, which is simpler, faster, and less costly than the single-plex fluorescence quantitative PCR method, and has better sensitivity, specificity, and repeatability. The viruses to be tested are fully enclosed for detection and single-tube real-time quantification, and the three poxviruses can be efficiently and quickly screened and identified according to the number of copies of the virus infection.

The invention relates to nucleic acid, a kit and a micro-fluidic chip for detecting natural epidemic disease pathogens. The nucleic acid comprises a primer pair and a probe for detecting forest encephalitis virus and / or Crimean-Congo hemorrhagic fever virus. The invention also provides micro-fluidic chips which are respectively coated and used for detecting the forest encephalitis virus and the Crimean-Congo hemorrhagic fever virus. The nucleic acid provided by the invention can be used for detecting a single forest encephalitis virus or a Crimean-Congo hemorrhagic fever virus, can also be used for simultaneously detecting the forest encephalitis virus and the Crimean-Congo hemorrhagic fever virus in a combined manner, and can be flexibly combined. The invention also provides a micro-fluidic chip for simultaneously detecting the forest encephalitis virus and the Crimean-Congo hemorrhagic fever virus, the use of the micro-fluidic chip can effectively save time, the detection cost is low, rapid screening is realized, and the detection method has strong specificity and high sensitivity and can effectively avoid missing detection.

The utility model belongs to the technical field of fertilizer processing devices, and particularly relates to an organic fertilizer particle screening device which comprises a base plate, a storage frame is fixedly connected to the upper surface of the base plate, supporting frames are fixed to the two sides of the storage frame, and two protection frames are installed on the supporting frames through bolts. And screening mechanisms are slidably connected to the inner sides of the two protective frames, each screening mechanism comprises a screening frame, rolling wheels are rotatably connected to the two sides of each screening frame, the rolling wheels are arranged on rail grooves correspondingly formed in the protective frames, and a screen is fixed to the bottom of each screening frame. The screening mechanism capable of moving in a reciprocating mode is arranged on the protection frame, organic fertilizer screening can be conducted when the screening mechanism moves left and right, the device is arranged in a multi-layer mode, organic fertilizer screening treatment can be conducted layer by layer, and the problems that an existing device is not high in screening efficiency, organic fertilizer particles cannot be screened layer by layer, and the screening efficiency is low are solved. And the device is inconvenient to move and use.