599results about How to "Quick screening" patented technology

The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.

The invention provides a method for screening toxic substances in a sample by using solid phase support liquid-liquid extraction-GC MC. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, centrifuging an extracting solution after extraction, collecting a supernatant and removing the organic solvent in the supernatant, and preparing a sample liquid; adding the sample liquid into an activated solid phase support liquid-liquid extraction column, performing standing, performing eluting by using an eluate, and collecting the eluate; carrying out a chromatogram-mass spectrometry of the eluate. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 150 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

The present invention relates to a method for detecting a target material in a sample, which method comprises the steps of: a) exposing a sample, expected to contain that target material, to at least two viruses which are capable of binding directly or indirectly with that target material so as to form a virally bound target material and to endow the virally bound target material with a distinctive property; and b) cultivating the product from stage a in the presence of an indicator material to which the viruses carried by the virally bound target material attach so as to cause the indicator material to adopt the distinctive property of the virally bound target material; and c) monitoring the presence or otherwise of virally attached indicator material. The invention also provides a kit for use in the method of the invention.

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.

The invention relates to a comprehensive verification system and method for identities and documentary evidences, and belongs to comprehensive credential verification systems and methods. According to the comprehensive verification system, a verification system website is established, and the verification system website serves as a verification center; the verification system website is connected with a terminal of the verification system website through a network to form a verification network system. Individual registration information and unit registration information are included in the verification system website, and are the bottom layer basic information of the verification system website. According to the method, a registration method and a verification method are included, unique verification email addresses are automatically generated by using identity numbers and organizing institution codes and serve as verification bases, and simple, convenient and reliable verification is achieved. Wrong identity cards, repeat identity cards and fake identity cards can be conveniently and fast found and screened, and the problem of train ticket wiped by ticket touts can be solved; fake official seals and credentials can be identified; the problem of social insurance and birth certificate verification can be solved.

The invention aims to provide an establishment method and application of a mass spectrum database of a prohibited colorant in foods. The method provided by the invention comprises the steps of: (1) establishing a first-level mass spectrum database and a second-level mass spectrum database of the colorants; and (2) separating the colorants with similar properties in the established mass spectrum databases. According to the establishment method and the application, the technical evaluation foundations are provided for the law enforcement; a purpose of fast screening the unknown prohibited added colorants is achieved by virtue of the mass spectrum databases; and the establishment method and the application can be helpful for strengthening the food detection of markets and carrying out preventive measures to effectively protect life safety of people.

The invention discloses a GC-MS non-target method used for rapid screening of plant differential metabolites, and belongs to the technical field of flavours and fragrances, and metabonomics study technology. According to the GC-MS non-target method, modern GC-MS full scanning technology is adopted to obtain chemical ingredient information of volatile substances in plant; multidimensional Gaussian smoothing is adopted to extract substance information in a TIC picture; dynamic programming algorithm is adopted to calibrate chromatographic peak time shifting among samples, realize aligning of chromatographic peaks belongs to one same substance, achieve comparability of substance information among different samples; nearest neighbor-clustering algorithm is adopted to realize compound registration; and at last variance analysis is adopted to screen compounds with significant difference from different groups. According to the GC-MS non-target method, compound mass spectrum information is output so as to obtain a MSP file; and the MSP file can be input into the NIST database to search compounds. The GC-MS non-target method can be used for locking compounds with dissimilarities in plant samples rapidly, and is suitable for rapid analysis determination of a large amount of samples.

An apparatus, module, methods and systems for automated, standardized assessment and analysis of a human olfactory system's odor detection ability as an indicator or predictor of cognitive impairment or change in cognitive health, and other health conditions such as diabetes. Notably, the present invention is operable for use across all age groups of humans and provides quantitative detection and analysis of a human olfactory system's detection ability compared to a relevant demographic population.

The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.

The invention discloses a general nucleic acid isothermal detection reagent of respiratory tract infection associated adenoviruses. The invention provides recombinase polymerase nucleic acid detection set nucleic acid which is used for identifying respiratory tract infection adenoviruses and is composed of a primer pair and a probe; the primer pair is composed of a single-stranded DNA (Deoxyribonucleic Acid) molecule represented by a sequence 1 in a sequence table and a single-stranded DNA (Deoxyribonucleic Acid) molecule represented by a sequence 2 in the sequence table; the probe is composed of A-dt-FAM-tetrahydrofuran-dt-BHQ1-B, wherein the nucleotide sequence of A is a sequence 3; the nucleotide sequence of B is a sequence 4. An experiment testifies that general RPA (Recombinase Polymerase Assay) detection primer and probe are designed aiming at six types of the respiratory tract infection associated adenoviruses; an RPA detection system and method for the respiratory tract infection associated adenoviruses are established; the technology can be used for carrying out field rapid screening and detection on the respiratory tract infection associated adenoviruses, has an important value and can realize rapid screening of unknown samples.

The invention provides a rapid detection method for water-soluble azo dyes in foods. The method comprises the steps of (A), sample extraction; (B), polyamide column concentration and purification; (C), elution of pigments; and (D) qualitative detection. According to the rapid detection method, the rapid screening can be timely and effectively carried out on suspected samples, the method is independent of large-scale instruments and equipments, and lawbreakers can be harshly attacked within the shortest time; and besides, the rapid detection method further has the advantages of short detection time, wide applicable range, high detection accuracy, low detection limit, strong anti-interference capacity and the like.

The invention relates to a detection method for redox materials in food, which is used for detecting the quantitative concentration of the residue redox materials in food in a short time. The method is implemented by a detecting test piece and a detector, and comprises the following steps: pretreating a proper amount of a food test sample, preparing a certain amount of the preteated food test sample, and dissolving the pretreated food test sample in buffer solution; subjecting the buffer solution containing materials to be detected and the formula layer of the detecting test piece to a redox reaction; the detector provides a reaction level to the reaction electrode of the test piece to acquire an electrical signal; and the content of the materials to be detected in the food test sample can be acquired by the electrical signal. The invention also provides the detecting test piece and the detector, which are applicable to the detection method.

The invention provides a method for screening toxic substances in a sample by using solid phase microextraction-GC-MS. The method comprises the following steps: extracting the sample by using a mixed solution containing a water-soluble organic solvent and deionized water, and producing an extract; centrifuging the extract, collecting a supernatant and removing organic solvents in the supernatant, performing centrifuging again, collecting a supernatant, and producing a sample liquid; inserting a PDMS extraction head into the sample liquid for carrying out solid phase microextraction, after extraction, fetching out the PDMS extraction head, inserting the extraction head into the GC-MS for desorption, and analyzing the desorbed sample which enters into a chromatographic column by using a GC-MS method. According to the invention, different toxic substances of a plurality of structures in a sample can be rapidly and effectively detected, especially screening of more than 60 kinds of toxic substances can be realized by single determination with high detection efficiency; the method provided by the invention is especially suitable for fields such as forensic science and has a good praticical value.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention discloses a novel fault attack method aiming at the Advanced Encryption Standard (AES-128) algorithm, which comprises the steps that: firstly, an attacker randomly selects a plaintext, acquires the correct ciphertext of the plaintext under the action of an initial secret key; and then the attacker encrypts the randomly selected plaintext, optionally selects any one of first three columns of sub-secrete keys at the ninth round in an encryption operation progress, performs multi-byte random fault induction to the one column of sub-secret keys, and acquires a wrong ciphertext comprising random faults; next, the attacker performs multi-byte random fault induction to the rest three columns of sub-secret keys at the ninth round through the same operation till the rest three columns of sub-secret keys are recovered completely and sub-secrete keys at the tenth round are acquired; at last, the initial secret key is acquired through reverse calculation. The novel fault attack method aiming at the AES-12 algorithm disclosed by the invention attacks the sub-secret keys at the ninth round and operates any column of the needed sub-secret keys without affecting operation results of each other, therefore, an AES-128 password system can be treated by differential fault attack by a plurality of equipments at the same time, thus acquiring initial secret key information rapidly and saving more time.

The invention provides a method for detecting and disposing an abnormal traffic attack, comprising the following steps: 1) filtering known abnormal traffic and normal traffic of a visitor IP address; 2) according to a current traffic numerical value of the traffic passing the filtration of the visitor IP address and a corresponding traffic template numerical value in a TOPN dynamic filtering table, distinguishing the abnormal traffic and the normal traffic passing the filtration in the visitor IP address; 3) discarding the abnormal traffic, and forwarding the normal traffic. The invention also provides a device for detecting and disposing the abnormal traffic attack, comprising a traffic receiving unit, a static filtering unit, a dynamic detection filtering unit and a self-learning unit connected orderly, wherein the dynamic detection filtering unit is connected with an operation and deciding unit and the self-learning unit. The method and the device provided by the invention can improve disposal efficiency and detection accuracy for abnormal behaviors, and can quickly detect and dispose the abnormal attack traffic without influencing normal traffic access.

The invention relates to a joint inspection kit for IgG and IgM antibodies of hepatitis C virus and a preparation method thereof. The kit adopts colloidal gold as markers; a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3) and a sample absorbing pad (4) are mutually overlapped and adhered on a bottom board (5) in sequence, wherein the colloidal gold pad (2) is made of glass fibers or non-woven fabrics with mixed antigens of the hepatitis C virus (HCV) marked by colloidal gold; and the nitrocellulose membrane (3) is coated by anti-human IgM antibodies and HCV mixed antigens respectively as detecting lines and is coated with anti-HCV antibodies as quality control lines. In the invention, a colloidal gold immunochromatography technology is adopted to determine the IgG and IgM antibodies of hepatitis C, and the IgG and IgM antibodies of the hepatitis C can be jointly inspected by one-time operation, so that the operation process is simplified. The invention has the characteristics that the operation is simple, the reaction is fast, the sensitivity is high and the economy and practicability are achieved and the like.

The invention provides a RNA probe capable of simultaneously detecting multiple neonatal hereditary diseases and a qualitative detection method in vitro of multiple neonatal genetic metabolic diseases, and the detection method provided by the invention is used for reducing the detection cost. Specifically, the invention discloses a RNA probe capable of simultaneously detecting multiple neonatal hereditary diseases, and the design method of the RNA probe comprises the following steps: 1) obtaining exon regions of genes corresponding to the neonatal genetic metabolic diseases; 2) regulating an oligonucleotide design principle. 1760 oligonucleotide sequences of the RNA probe provided by the invention are specifically as shown in table 1. The invention further provides a kit containing the RNA probe, and the kit can be used for simultaneously detecting multiple neonatal hereditary diseases.

Provided are a method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application. An ion mobility spectroscopy is combined with real-time direct analysis of ion source time-of-flight mass spectrometry, and a database is established and includes reduced mobility (k0) obtained by the ion mobility spectroscopy and also includes a mass number of a quasi-molecular ion peak generated by real-time direct analysis of the ion source time-of-flight mass spectrometry, as well as a mass number of a fragment ion peak generated by a collision induced ionization mode, the obtained database is utilized to perform high-throughput drug screening, sample processing is simple, no chromatographic separation is required, test results are accurate, reliable and easy to operate, and fake positive results are avoided. The established rapid screening method can be used for the rapid detection of multiple drugs with the detection limit range of 0.02-2 micrograms/mL.

The invention provides an industrial product quality real-time monitoring method, equipment and system, relates to the technical field of networks, and aims to reduce the detection delay, facilitate industrial control equipment to quickly screen industrial products according to an analysis result of an edge cloud platform, improve the detection precision and the working efficiency and reduce the production cost of enterprises. The method is applied to an edge cloud platform, the edge cloud platform is in communication connection with industrial control equipment and a central cloud platform, and the method comprises the following steps: receiving image data of an industrial product sent by the industrial control equipment; receiving a detection model sent by the central cloud platform; utilizing the detection model to analyze and process the image data to obtain an analysis result; and feeding back the analysis result to the industrial control equipment to enable the industrial controlequipment to screen the industrial products according to the analysis result. The method is mainly used for monitoring the quality of industrial products in real time.