1834results about How to "Efficient screening" patented technology

Methods are described for detecting protein-protein interactions, among two populations of proteins, each having a complexity of at least 1,000. For example, proteins are fused either to the DNA-binding domain of a transcriptional activator or to the activation domain of a transcriptional activator. Two yeast strains, of the opposite mating type and carrying one type each of the fusion proteins are mated together. Productive interactions between the two halves due to protein-protein interactions lead to the reconstitution of the transcriptional activator, which in turn leads to the activation of a reporter gene containing a binding site for the DNA-binding domain. This analysis can be carried out for two or more populations of proteins. The differences in the genes encoding the proteins involved in the protein-protein interactions are characterized, thus leading to the identification of specific protein-protein interactions, and the genes encoding the interacting proteins, relevant to a particular tissue, stage or disease. Furthermore, inhibitors that interfere with these protein-protein interactions are identified by their ability to inactivate a reporter gene. The screening for such inhibitors can be in a multiplexed format where a set of inhibitors will be screened against a library of interactors. Further, information-processing methods and systems are described. These methods and systems provide for identification of the genes coding for detected interacting proteins, for assembling a unified database of protein-protein interaction data, and for processing this unified database to obtain protein interaction domain and protein pathway information.

An automated and interactive system that facilitates efficient capitalization / liquidation and monitoring of private and publicly-traded enterprises through a network-driven marketplace is disclosed. The system may be comprised of a dynamic process for enterprise characterization, a customizable computational engine that utilizes statistical reference information to quantify a multi-factor scoring value for each unique enterprise, a customizable system for investor-users to filter, rank, and screen enterprise prospects, a customizable system for monitoring the performance of enterprises, an integrated internal system for electronic communication between market participants, and an empirical feedback system that provides a dynamic knowledge base of statistical reference information for various computational components of the invention.

A new method of cell fixation using a mild denaturing heat and the fixative PermiFlow™ or similar fixative that preserves cell morphology, light scatter profiles, nucleic acid content, cell surface epitopes and unmasks internal epitopes previously not available. The method can be combined with analysis of each of these parameters by flow cytometry and thus has application in drug screening and patient or tumor monitoring protocols.

A corrosion and ultra violet-resistant silver mirror for use in solar reflectors; the silver layer having a film-forming protective polymer bonded thereto, and a protective shield overlay comprising a transparent multipolymer film that incorporates a UV absorber. The corrosion and ultraviolet resistant silver mirror retains spectral hemispherical reflectance and high optical clarity throughout the UV and visible spectrum when used in solar reflectors.

The present invention discloses a series-wound long short-term memory recurrent neural network-based heating load prediction method. The method comprises the steps of constructing a sample data set based on temperature, climate and heat supply data during a given period of time, and respectively subjecting the input data and the output data of the sample data set to standardized treatment; dividing the input data into two portions, respectively inputting the two portions into two independent long short-term memory recurrent neural networks to merge the two portions of the input data, inputting the output data to a long short-term memory recurrent neural network at a next layer, and finally inputting the data into two full connection layers; training a constructed series-wound long short-term memory recurrent neural network, and optimizing the network by adopting the parameter optimization-based adaptive torque estimation algorithm; inputting to-be-predicted data into the series-wound long short-term memory recurrent neural network, calculating and obtaining a heating load prediction result. The method of the invention can effectively discriminate input data, and accelerate the learning speed. Therefore, the learning efficiency is improved and the prediction accuracy is improved.

A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities are immobilized in these cells, which entities are effective to selectively hybridize to or sequester a target biomolecule. Different binding entities are immobilized in different cells in an array to create a biochip that can be used to assay for a number of target biomolecules.

The present invention relates to methods of making fiber-reinforced molded articles and fiber mats, wherein the methods use, inter alia, anaerobic binders.

The present invention includes a method and apparatus for high sensitivity whole body scanning using MR imaging. The invention includes acquiring MR data as the patient moves through the iso-center of the magnet while providing interactive control for the operator to change scan parameters and table motion and direction. The technique allows efficient whole body scanning for fast screening of abnormalities while allowing operator control during the screening process to interrupt table motion and redirect the speed and direction of the table while also allowing control over the acquisition plane, number of sections imaged, inter-section spacing, and the scan location.

A system and method for processing data streams is disclosed. The system receives data packets for data streams, screen the data packets for searched patterns, and forward the data packets for their respective stream processing. Generally, the data packet is scanned for viruses before being forwarded for further processing. When an out-of-order data packet is received, a copy is made and the data packet is forwarded without being scanned. When a delayed data packet is received, it is scanned for virus along with the saved copy of the out-of-order data packet. If a virus is detected, the delayed packet is dropped and its connection reset. If no virus is found, the delayed packet is forwarded for further processing.

The invention is drawn to composite agarose / acrylamide compositions and gels. In particular it relates to gels for the separation of molecules, particularly macromolecules such as proteins. The invention is also directed to the preparation of composite gels, the separation of molecules by techniques such as electrophoresis using such gels, and the transfer of proteins from such gels to a transfer membrane using an immunoblot transfer gel.

The invention provides a member authentication-management method, device and system based on face identification. The method comprises that an infrared camera device is used to monitor a scene within a specific area; when a consumer image is found in the monitored scene, a face image of a present consumer is extracted from the consumer image; whether there is a living body in the specific area is detected, and the face image matches and is compared with a member face image pre-stored in a data base; when it is detected that there is a living body in the specific area and a comparison result shows that the present consumer belongs to members, the present consumer is reminded of member identity information and consumption information; when it is detected that there is living body in the specific area but the comparison result shows that the present consumer does not belong to members, the present information is reminded of consumption information and member joining prompt information; and no operation is carried out when it is detected there is no living body in the specific area. The method, device and system do not influence by illumination conditions, and the consumer himself / herself can be effectively discriminated from a picture / video.

The invention provides a malicious user identification method and apparatus. The method comprises the following steps: obtaining bullet screen sending behavior features of a plurality of users; calculating a similarity value of each two users in the plurality of users according to the bullet screen sending behavior features; setting a suspect degree value of a malicious user as a first initial suspect value, and setting the suspect degree value of a to-be-identified user as a second initial suspect value; for each user, performing iterative computation on the suspect degree value of the user through a probabilistic graphical model according to the current suspect degree value of the user and the similarity value of the user and the other users; and after multiple iterative computations, using the to-be-identified user with the suspect degree value greater than a suspect threshold as the malicious user. Therefore, according to the association of the to-be-identified user and the determined malicious user on the bullet screen sending behavior features, the malicious users with unobvious malicious behavior features can be effectively screened out.

A method of screening EEPROMs for data retention quality employs a UV source which is arranged to be impinged upon the devices while in wafer form, at or near an electrical probe station. Known data is stored in memory cells on an EEPROM chip while the chip is in wafer form, at a probe station. The wafer is then moved beneath a UV silo near the probe station and exposed to UV light, for a period of time and at an intensity which is sufficient to cause leakage of charge from potentially leaky floating gates. The wafer is again subjected to electrical probe where the amount of change in retained charge is detected. From this test, an indication of the charge retention ability of the devices is obtained. The UV light increases the energy state of the stored charge thus accelerating the decay of the stored charge located on the floating gates in the EEPROM device. Bits that have inherent leakage paths decay more rapidly.

A vibratory screening machine includes a primary screening area and a pre-screening assembly including a frame and screen assemblies. The screen assemblies are secured to the frame and form a pre-screening surface. The frame is attached to the vibratory screening machine such that the concave pre-screening surface is above the primary screening area.

Signal Transducer and Activator of Transcription (STAT) proteins have a fundamental role cell signaling, and are activated by a large number of cytokines and growth factors. One member of the STAT family, STAT3, has a critical role in oncogenesis. The present invention relates generally to disruption of the pathway of STAT3 signaling in the treatment of human cancer. STAT3 activation is shown to be present in diverse tumor cell lines and tumors, to promote oncogenesis, to inhibit apoptosis, and to reduce sensitivity to chemotherapeutic agents. Inhibition of STAT3 signaling induces apoptosis specifically in tumor cell lines, and increases sensitivity to chemotherapeutic agents. The invention relates more particularly to methods, compositions, means of administering such compositions, and means for identifying such compositions for the inhibition of STAT3 intracellular signaling in the treatment of human cancers.

The scheme of the invention discloses a vehicle assistance driving system based on the infrared thermal imaging and millimeter wave radar technology. The main functions include pedestrian and vehicledetection, image and sound fusion and collision early warning in driving of the vehicle. The early warning system uses the following technical characteristics: the infrared camera and the millimeter wave radar specialized for vehicle-mounted assistance driving, the data acquired by the radar, such as the distance, the direction and the speed and the images acquired by the infrared camera are fusedfor screening and determining the pedestrians or the vehicles, the pedestrian or vehicle characteristics are extracted through the algorithm, finally the result is displayed on the visual interface and multidimensional early warning information including images, numbers and sound is provided.

The disclosure provides an apparatus and method for assessing brain plasticity by measuring electrical brain biomarkers, for example, with a near real-time analysis of electrical brain biomarkers, where an increase or decrease in at least one biomarker is indicative of a state of brain plasticity in response to a stimulus or treatment. Brain plasticity can be measured with or without an added stimuli, for example, to determine the best time for learning. Also provided is a method for treating a neurological disease or trauma by applying an electrical or drug stimulus to a patient, where the stimulus is increased or decreased depending on the changes of electrical brain biomarker of the patient. This treatment can occur in near real-time, so a course of treatment can be tailored immediately to a patient's needs.

The invention relates to the Internet application technology field, and especially relates to an NAT (Network Address Translation) flow identification method based on a transmission layer flow characteristic in Internet application. The method comprises the following steps: acquiring a data packet which goes through an NAT device as training data; learning a classifier in the training data; sending all subsequent acquired data packets which go through the NAT device to the classifier; according to a characteristic of a data flow of the sending data packets, distinguishing an NAT flow from a non NAT flow by the classifier. The NAT flow identification method is independent of an operation system or a passive identification method of any special field, has a wide application range and is noteasy to fail. Through carrying out characteristic extraction and characteristic selection on a network flow, an optimal characteristic subset of carrying out NAT identification is effectively screened out. According to the screened optimal characteristic subset, data is divided into different clusters through a cluster algorithm, an application type of a cluster is determined by utilizing a few labeled data, and the NAT identification is realized.

The present invention includes a method and apparatus for high sensitivity whole body scanning using MR imaging. The invention includes acquiring MR data as the patient moves through the iso-center of the magnet while providing interactive control for the operator to change scan parameters and table motion and direction. The technique allows efficient whole body scanning for fast screening of abnormalities while allowing operator control during the screening process to interrupt table motion and redirect the speed and direction of the table while also allowing control over the acquisition plane, number of sections imaged, inter-section spacing, and the scan location.

The invention discloses an accurate liquid extracting and metering device and a method. The device comprises a pump, a valve unit, an electromagnetic stop valve, a liquid storing unit, a testing module, a liquid detector A and a liquid detector B, wherein the valve unit is provided with a liquid inlet passage, a testing passage, a liquid discharging passage and a common passage connected with theliquid storing unit; the liquid inlet passage of the valve unit is connected with liquid; the testing passage is connected with the testing module; the liquid discharging passage is connected with a waste liquid collecting device; the common passage is connected with one end of the liquid detector A by the electromagnetic stop valve, and the other end of the liquid detector A is connected with oneend of the liquid storing unit; the other end of the liquid storing unit is connected with one end of the liquid detector B, and the other end of the liquid detector B is connected with the pump.

The invention discloses a file management method of electronic equipment and the electronic equipment. The method comprises the following steps: detecting a deletion operation in allusion to a first file, wherein the first file is stored in a first storage area of the electronic equipment; determining whether the attribute of the first file accords with a preset attribute; when the attribute of the first file accords with the preset attribute, moving the first file from the first storage area to a second storage area; and redirecting the first file to obtain a storage path of the first file in the second storage area, wherein the first file is accessed through the storage path. The method provided by the invention is used for solving the technical problem that related applications cannot run normally as the files cannot be accessed after being deleted, so that the technical effect that the deleted files are temporarily stored and then the related applications still can normally access to the files is realized.

A biochip is formed with a plurality of optically clear hydrogel microspots attached to the top surface of a solid substrate in the form of an array. Each of the microspots is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities or probes are immobilized in these microspots, which entities are effective to selectively hybridize to or sequester target biomolecules, such as target cells. Different binding entities are immobilized in microspots in different regions of an array to create a biochip that can be used to assay for or separate a number of target biomolecules, such as cells from maternal blood.

The invention provides a reagent for diagnosing cervical cancer, which can not only detect the presence or absence of cervical cancer but can also distinguish squamous cell carcinoma and adenocarcinoma from each other, a method for screening cervical cancer by using the reagent, particularly a screening method capable high speed processing by utilizing flow cytometry. The reagent comprises a first labeled antibody reacting with gland cells, a second labeled antibody reacting with adenocarcinoma cells and a third labeled antibody reacting with atypical cervical squamous cells, the antibodies being labeled with mutually distinguishable labels respectively. Preferably, at least one selected from the group consisting of MUC1 antibody, cytokeratin 7 antibody, and cytokeratin 18 antibody is used as the first labeled antibody, at least one selected from the group consisting of cytokeratin 8 antibody and HIK1083 antibody is used as the second labeled antibody, and at least one member selected from the group consisting of NMP179 antibody, p16INK4A antibody, Ki-67 antibody, p53 antibody, p21 antibody, EMA antibody, CEA antibody and MIB-1 antibody is used as the third labeled antibody.

The invention relates to a distance measuring method of a significant target in a binocular image. The distance measuring method of the significant target in the binocular image aims to solve the problem that an existing target distance measuring method is low in the processing speed. The method includes the steps that step 1, significant feature extraction is conducted on the binocular image through a visual significance model, and a seed point and a background point are marked; step 2, a weighed graph is established for the binocular image; step 3, a significant target in the binocular image is partitioned through a random walk image partitioning algorithm by means of the seed point and the background point in step 1 and the weighed graph in step 2; step 4, key point matching is conducted on the significant target separately through an SIFT algorithm; step 5, the significant target distance is worked out by applying a parallax matrix K' worked out in step 4 into a binocular distance measuring model. The distance measuring method can be applied to distance measurement of the significant target of the image in front of the vision in the intelligent automobile running process.

The invention relates to the technical fields of biological detection, environment monitoring and clinical detection, in particular to a micro-fluidic chip and a manufacturing method thereof, which are used for overcoming the defects of large sample using amount and complex operating steps existing in the biological detection process in the prior art. The invention provides a micro-fluidic chip and a manufacturing method thereof. The micro-fluidic chip comprises a substrate layer, a functional layer and a micro-fluidic pipeline layer, wherein the functional layer is fixed on the substrate layer, and comprises at least two parallel functional strips; the functional strips are strip-shaped bands formed by a material which can react with a specific material; the micro-fluidic pipeline layer is provided with at least two pipelines; the pipelines are communicated with at least one functional strip; and the two ends of each pipeline are provided with through holes which are communicated with the atmospheric air. According to the micro-fluidic chip provided by the invention, various different substances in a plurality of samples can be detected simultaneously. A detection method provided by the invention has the advantages of easiness for operating, small reagent using amount, saving in time, high efficiency and accuracy.

A vibratory screening machine includes a primary screening area and a pre-screening assembly including a frame and screen assemblies. The screen assemblies are secured to the frame and form a pre-screening surface. The frame is attached to the vibratory screening machine such that the concave pre-screening surface is above the primary screening area.

The present invention describes novel beta-sheet proteins having specific binding properties and catalytic properties and also methods for preparing these proteins.

Method for the identification of a gene in a transposon population, comprising isolating genomic DNA, optionally pooling the DNA, restrict the DNA in the pools using an enzyme, ligate adaptors, amplifying the adaptor-ligated fragments with primers, one of which is a primer complementary to a border of a transposon sequence, high throughput sequencing of the fragments, aligning the fragments with known sequences in a database and thereby identifying gene candidates.