316 results about "Culture fluid" patented technology

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.

The invention belongs to the technical field of mushroom cultivation, and discloses an earthing cultivation mushroom cultivation box which is used as an independent unit for fermentation, mushroom cultivation and fruiting of earthing cultivation mushrooms and comprises an open box body and an aeration layer arranged at the bottom of the box body, and an inner air channel and an inner water channel are embedded in the box body. The inner air channel is provided with an aeration port in the aeration layer in the box body, gas entering the aeration layer from the aeration port enters a culture medium covering the aeration layer from an opening in the surface of the aeration layer, and the inner water channel is provided with an inner nozzle for spraying a culture solution into the box body at the upper part of the culture medium in the box body. The movable platform is used for bearing a plurality of box bodies to form a culture module, and a main air passage and a main water passage are arranged at the lower part of the movable platform and are used for supplying air and water to each opposite box body. The cost is reduced.

The invention relates to a method for electrically activating oocytes based on nanosecond pulses. Single oocytes are sequentially and uniformly arranged between two parallel electrodes of an electrode dish, the electrode dish is characterized in that the distance between the electrodes is 1 mm, the electrodes are placed in a culture dish, and the two ends of the two electrodes are sealed; a culture solution is firstly put between the electrodes which are arranged in parallel, mineral oil is covered to prevent the culture solution from being evaporated, after preheating treatment, oocytes are sequentially and uniformly put between the two electrodes which are arranged in parallel one by one, and then nanosecond pulse electric activation treatment is applied. The electrodes which are arranged in parallel are adopted, the oocytes are sequentially and uniformly placed between the electrodes in a single mode, the situation that the electric pulse effect is reduced due to adhesion and stacking of all the cells is avoided, the oocytes receive uniform and equivalent electric pulse sequences, the action effect and stability of electric pulses on the cells are obviously improved, and the operation efficiency of the oocytes is improved. And the reliability and reproducibility of a test conclusion are improved.

The invention discloses a multi-layer uniform mixing method for microorganism culture, which comprises the following steps: putting: putting microorganisms to be cultured and a culture solution into test tubes, and putting the test tubes into a plurality of culture cavities arranged in a culture box layer by layer in batches; temperature regulation: independently regulating and controlling the temperature in the plurality of culture cavities by a temperature regulation and control mechanism; uniformly mixing: the test tubes revolve and rotate in the incubator, and meanwhile, the test tubes are pushed to oscillate in a small amplitude under the action of an oscillation mechanism, so that the microorganisms and the culture solution are uniformly mixed. The incubator is provided with a plurality of culture cavities, bin doors are hinged to the front sides of the culture cavities, the incubator comprises trays, rotating discs, placement grooves, oscillation blocks and a temperature regulation and control mechanism, the trays are installed in the culture cavities in a sliding mode, the rotating discs are rotatably arranged at the tops of the trays, and the placement grooves rotate while rotating along with revolution of the rotating discs. The invention is used for solving the problem of inaccurate detection result caused by poor mixing effect of the existing mixing device.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The utility model belongs to the technical field of microbial agent culture, and particularly relates to a microbial agent improved saline-alkali soil culture device which comprises a culture box and a shaking assembly arranged in the culture box, the shaking assembly comprises a rotating disc, a transmission rod, a supporting plate, a stabilizing frame, a lap joint frame and a layered net frame, a transmission groove is formed in the bottom face of the transmission rod, and the supporting plate is arranged in the transmission groove. And the supporting plate is located on the upper side of the transmission rod, a rolling contact rolling wheel is arranged on the bottom face of the supporting plate, and the rolling wheel is rotationally installed on the upper side of the transmission rod. The culture dishes in the culture box can be shaken in different states through the shaking assembly, so that a culture solution is always kept in a dynamic mixing state, the dynamic environment of the saline-alkali soil is simulated, precipitation of saline-alkali components is avoided, the activity and reproduction efficiency of a microbial agent are improved, and the improvement effect of the microbial agent on the saline-alkali soil is enhanced.

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

A plurality of liquid storage bags and waste liquid bags are arranged in a liquid storage area, a liquid separation chip and a plurality of peristaltic pumps and electromagnetic valves are arranged in a liquid separation area, four reaction chips are arranged in a reaction area, each reaction chip can support two parallel experiments, and the different areas are connected through hoses. A culture solution in the liquid storage area is controlled by a pump valve and a liquid separation chip in the liquid separation area, flows into different reaction chips, is subjected to microbial culture and interaction experiments with materials, and finally flows into a waste liquid bag. The reaction chip can be used for collecting electrochemical signals, and real-time observation can be carried out through a microscope. The chip card box provided by the utility model is designed to be totally closed, is high in integration level, can support simultaneous operation of a plurality of reactions, and is safe and reliable.

The invention discloses an organoid culture monitoring device which comprises a culture layer and a plurality of sensing modules, the culture layer is provided with a plurality of culture grooves used for culturing organoid, and the culture grooves are filled with conductive culture liquid. Each sensing module is arranged in one culture tank, and the sensing end of the sensing module is submerged in the culture solution and is used for monitoring the growth condition of the organoids in the culture tanks; the sensing module comprises a first insulating substrate and a detection unit, the first insulating substrate is attached to the groove wall of the culture groove in a matched mode so as to surround the organoid located in the culture groove or cells used for culturing the organoid, and the detection unit is arranged on the side, facing the organoid or the cells, of the first insulating substrate; and a groove-shaped sensing interface surrounding the cells or the organoids is formed in the culture groove. According to the technical scheme, the groove-shaped sensing interface is formed, so that non-contact, non-destructive, in-situ and long-time-history synchronous monitoring of the organoids is effectively realized.

The utility model discloses an escherichia coli culture device capable of supplementing materials for multiple times. The Escherichia coli culture device capable of supplementing materials for multiple times comprises a culture dish box and a culture dish cover, an isolating inner ring cylinder is integrally mounted in the culture dish box, an overflow port is formed in the isolating inner ring cylinder, and a first isolating plate and a second isolating plate are integrally mounted on the isolating inner ring cylinder and the inner wall of the culture dish box. According to the Escherichia coli culture device capable of supplementing materials for multiple times provided by the utility model, the isolation inner ring cylinder is arranged in the culture dish box, and the first isolation plate and the second isolation plate are arranged between the culture dish box and the isolation inner ring cylinder, so that a liquid discharge cavity is formed among the isolation inner ring cylinder, the first isolation plate, the culture dish box and the second isolation plate; and the bottom of the liquid discharge cavity is arranged to be an inclined plane, so that the discharged culture liquid can be automatically and continuously collected towards the liquid extraction tank, and the residue of the waste culture liquid can be reduced as much as possible.

The utility model relates to and discloses a microbial fermentation culture container which comprises a fermentation tank body, the fermentation tank body comprises an outer shell inner layer and an inner shell, the inner shell is arranged in the outer shell inner layer, a barrel-shaped temperature adjusting cavity is reserved between the outer shell inner layer and the inner shell, heat conduction liquid is filled in the barrel-shaped temperature adjusting cavity, and a water heater is further installed in the barrel-shaped temperature adjusting cavity. According to the microorganism bacterium fermentation culture container, the water heater can generate heat to heat water in the barrel-shaped temperature adjusting cavity by being communicated with a circuit in the water heater, and then the heated water provides extra heat for microorganism bacterium liquid culture liquid in the barrel-shaped temperature adjusting cavity through the inner shell, so that the needed fermentation temperature is maintained; when the water temperature in the barrel-shaped temperature adjusting cavity is not uniformly distributed, the inner shell can be driven to rotate in the inner layer of the outer shell by connecting a circuit in the motor, and the rotating inner shell is used for simultaneously stirring the microbial bacterium liquid culture solution and the water in the inner ring and the outer ring, so that the microbial bacterium liquid culture solution and the water in the inner ring and the outer ring are uniformly heated.

To provide a culture apparatus in which microalgae do not accumulate on the inner bottom surface of the containment section. [Solution] The culture apparatus 10 comprises a containment section 20 capable of containing culture solution CS and microalgae, a guide section 40 for guiding culture gas G, and a gas supply section 30 for supplying culture gas G to the culture solution CS. The supply pipe section 36 constituting the gas supply section 30 has a main pipe section 362 extending along the depth direction of the containment section 20 and a sub-pipe section 363 extending along a protruding direction intersecting the depth direction. The outlet 38 formed in the sub-pipe section 363 is located below the lower end 43 of the guide section and is formed in a portion of the sub-pipe section 363 that protrudes in the protruding direction from the lower end 43 of the guide section.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

A flow control tray for an algae culture fluid, comprising: a base layer extending from a first end to a second end in a base plane; and a plurality of ridges extending from the substrate layer in a first direction, where the substrate layer is adapted to direct flow of an algae culture solution above the substrate plane from the first end to the second end to form an overall flow direction parallel to the substrate layer, where each of the plurality of ridges includes a support, the support exits the substrate plane in a direction perpendicular to the substrate plane and is adapted to support an algae culture membrane wherein each of the plurality of ridges has a three-dimensional shape preferably adapted to bypass the culture fluid in the overall flow direction.

The invention discloses an in-vitro real-time electromagnetic exposure experiment device based on a conformal antenna. The in-vitro real-time electromagnetic exposure experiment device comprises the following steps: 1) designing the conformal antenna as a radiation source by using a formula; 2) placing the conformal antenna on a light through hole of a microscope objective table; 3) obtaining parameters of the culture solution model by using a meniscus formula, and establishing a culture solution model with a meniscus; 4) placing the circular culture dish filled with the culture solution in the middle of the conformal antenna; and 5) carrying out electromagnetic simulation on the conformal antenna parameters, the circular culture dish and the culture solution through software, carrying out grid division on the culture solution, and finally obtaining SAR distribution in the culture solution. Results show that the method can record the physiological activities of the cells in real time, and meanwhile, the SAR uniformity is also obviously improved.

The application relates to the technical field of microbial fermentation, and discloses a jet stirring device for microbial fermentation and a fermentation tank thereof. The jet stirring device comprises a stirring mechanism, and the stirring mechanism comprises a stirring main shaft. The bottom outer side of the stirring main shaft is provided with a first stirring structure capable of rotating and stirring the fermentation substrate and lifting the fermentation substrate. The middle outer side of the stirring main shaft is provided with a second stirring structure matched with the first stirring structure and capable of disturbing the flow of the lifted fermentation substrate. In the application, the opposite jet flow design (opposite rotating directions but periodic synchronization) of the first jet flow hole and the second jet flow hole is adopted to realize the forced collision and mixing of the fermentation bottom liquid and the culture liquid. Compared with the mixing mode of the prior art which only depends on the mechanical rotation of the stirring blade and the swinging of the tank body, the stability of the tank body is ensured, and the fermentation efficiency is improved.

The invention provides a preparation method of salicornia europaea bio-salt. The preparation method comprises the following steps: step 1, pretreating raw materials: preparing a culture solution from salicornia europaea raw materials; step 2, mixing and extracting; step 3, sterilization; step 4, inoculating bacillus subtilis; step 5, fermenting; step 6, centrifuging; step 7, carrying out solid-liquid separation; 8, clarifying: removing impurities from the liquid obtained after solid-liquid separation in the step 7 by using a natural clarifying agent, so that the solution is clear and transparent; and 9, crystallizing to obtain the salicornia europaea biologic salt. According to the method disclosed by the invention, the bio-salt is prepared by coupling bacillus subtilis fermentation with ZTC 1 + 1 natural clarifying agent clarification, so that zero-emission, zero-pollution and zero-load environment-friendly manufacturing is realized.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The present application is an algal culture device for culturing algae by flowing a culture solution, the algal culture device comprising: a columnar carrier having at least an outer surface of a porous material; a carrier rotating mechanism for rotating the columnar carrier around a central axis in the length direction; a culture solution receiving portion provided below the columnar carrier; a culture solution supply mechanism for supplying the culture solution to the columnar carrier; and a culture solution circulating mechanism for circulating the culture solution accumulated in the culture solution receiving portion to the culture solution supply mechanism. A scraper can be further provided, which is configured to work along the outer surface of the columnar carrier in a manner to recover algae generated on the outer surface of the columnar carrier. The porous material can be ceramic at least in part. The columnar carrier can be cylindrical at least in part. The columnar carrier can be tubular at least in part.

The aim of the present invention is to provide a plant culturing apparatus and a plant culturing method for a plant whole body that allow liquid phase roots and vapor phase roots to separately appear in the root system thereof without using soil. More specifically, according to the present invention, the above described aim is achieved by culturing a plant whole body in the plant culturing apparatus for a plant whole body, which comprises a light-shading casing that is provided with at least one through hole on the ceiling surface and holds a culture liquid, and an inner surface showing capillary force, that are arranged on at least a part of an inner side surface from among an inner surface of the light-shading casing.

The invention discloses a daphnia magna cultivation device and system, and relates to the technical field of aquatic organism cultivation, the daphnia magna cultivation device comprises a daphnia magna cultivation box, a first screening plate and a partition plate, the bottom end of one side of the daphnia magna cultivation box is provided with a first outlet, the first outlet is provided with a first valve, and the daphnia magna cultivation box is provided with a first light source close to the first outlet; a second light source is arranged far away from the first outlet, and the first screening plate and the partition plate are detachably mounted in the daphnia culture box; when the device is used, the first outlet is in a closed state, the functions of daphnia magna screening and culture solution replacement can be achieved at the same time by controlling opening and closing of the first light source and the second light source and installing the first screening plate and the partition plate in the daphnia magna culture box, the screening efficiency is improved, a straw is not needed to suck a disturbance object, damage to the disturbance object is reduced, and the practicability is high. The daphnia magna cultivation system can also achieve the functions of feeding and culture solution filtering and recycling.

The invention belongs to the technical field of tissue engineering, and relates to a centimeter / decimeter-level three-dimensional vascularization tissue engineering organoid culture device which comprises a culture room, a semipermeable membrane microtube and a peristaltic pump, the semi-permeable membrane microtube is positioned in the inner cavity of the culture device; the inner ends of the culture solution inlet and outlet are respectively communicated with two ends of the semi-permeable membrane microtube; the inner ends of the vascular nutrient solution inlet and outlet are respectively communicated with the inner cavity of the culture chamber; the outer ends of the culture solution inlet and outlet are respectively communicated with a peristaltic pump and a waste liquid collector; the outer end of the blood vessel nutrient solution inlet and outlet is respectively communicated with another peristaltic pump and a waste liquid collector; the vascularization tissue engineering organoid culture system is constructed through the design of double independent circulation systems and adjustable shearing force, oxygen and nutrition can be effectively provided for centimeter / decimeter-level tissue engineering organoid in vitro, metabolites can be taken away, meanwhile, a vascular system can be formed in the cultured tissue engineering organoid, and the tissue engineering organoid can be cultured in vitro. And an efficient and controllable in-vitro culture platform is provided for vascularized tissue engineering organs.

The utility model provides a microalgae culture system which comprises a photosynthetic reaction module, a growth adjusting module, a harvesting module and an oxygen discharging and dead algae separating module, the photosynthetic reaction module is provided with a light-transmitting disc tube, the growth adjusting module is provided with an adjusting groove, and the interior of the adjusting groove is divided into bent flow channels through a plurality of partition plates; the culture solution is subjected to high-intensity photosynthetic reaction in the photosynthetic reaction module and then enters the growth regulation module to grow in an environment with the intensity lower than that of the photosynthetic reaction of the photosynthetic reaction module, and then the algae in the culture solution are harvested by the harvesting module after the algae grow to a harvesting standard.

The utility model discloses a novel microalgae air purification oxygenation device which comprises a shell, a modular filter chamber and a microalgae incubator, wherein the modular filter chamber and the microalgae incubator are arranged in the shell; the shell is provided with an air outlet and an air inlet; the modular filter chamber comprises a plurality of filter layers and a photocatalyst coating purification chamber; the microalgae culture device comprises a microalgae culture solution water tank, a fan case, an aeration head and a liquid discharge pipe, the bottom of the microalgae culture solution water tank is sealed, and the top of the microalgae culture solution water tank is communicated with the air outlet; a light source is arranged at the bottom of the microalgae culture solution tank; the fan case is provided with a centrifugal fan, the aeration head is arranged in the microalgae culture solution water tank and is communicated with the fan case through an air pipe, and the fan case is communicated with the photocatalyst coating purification chamber; the bottom of the microalgae culture solution water tank is communicated with a liquid outlet formed in the lower part of the shell through a liquid outlet pipe. The internal structure is optimized, microalgae culture, microalgae purification, filtration, photocatalyst purification and the like are combined, microalgae air purification is achieved, and the air purification effect is improved.

The invention provides a method for detecting forskolin, Y-27632 and AM2394 in cell culture biomass and a culture solution, and relates to the technical field of food detection. The detection method comprises the following steps: detecting target objects in cell culture biomass and a culture solution by using an ultra-high performance liquid chromatography-tandem mass spectrometry, and controlling a mobile phase A to be ultrapure water, formic acid and ammonium acetate in a detection process; the mobile phase B is methanol or acetonitrile; the detection method can accurately detect forskolin, Y-27632 and AM2394 in the cell culture biomass and the culture solution and perform quantitative analysis, and meanwhile, the detection method is simple and rapid to operate, high in sensitivity and good in repeatability.

The invention discloses a method for realizing long-acting stable sensing by controlling the thickness of an electroactive biological membrane by using NO. The method is used for solving the problem of signal drift caused by uncontrollable thickness in long-term culture of the electroactive biological membrane. The method comprises the following steps: firstly, building a culture system consisting of a closed reactor, an electrode and a culture solution; then, by taking the domestic sewage centrifugal thalli as an inoculation source, forming an initial electroactive biological membrane by applying a constant voltage of 0.5-0.9 V; and finally, after 2-3 cycles of pre-culture, 200-800 [mu] M of DETA NONOate is added into the system as an NO donor to continuously inhibit the thickening of the biological membrane, so that the thickness of the biological membrane is stabilized at 40-80 [mu] m, and meanwhile, the metabolic activity of electricity-producing bacteria is maintained, and it is ensured that the multi-cycle maximum sensing current variation coefficient is lower than 5%. The NO regulation and control strategy is creatively utilized, the structural stability and the signal reproducibility of the electroactive biological membrane are cooperatively optimized, a sensing element capable of stably operating for a long time is constructed, and a low-maintenance and high-reliability biological sensing solution is provided for real-time monitoring of pollutants in a complex water quality environment.