627 results about "Culture fluid" patented technology

The invention discloses an intelligent pretreatment workstation for microbiological detection, and relates to the technical field of microbiological detection. The module comprises a culture dish turntable, a transmission part, a homogeneous slapping part, a vortex oscillation part, a material rack storage part, a culture medium feeding part and the like; the culture dish turntable module comprises an upper dish station, a liquid injection station, an inoculation / coating station, a lower dish station and the like, as well as a transplanting electric module and a shaking-up module; the liquid injection unit can be used for injecting three culture solutions; the culture dish conveying module is responsible for conveying culture dishes to corresponding stations and is connected with an external feeding and receiving unit; the material rack storage module is used for storing pipettor tips, test tubes and homogenizing bags; the homogeneous slapping and vortex oscillation module is used for sample dilution and homogenization; the clamping robot is responsible for conveying the homogeneous bag and the test tube to the corresponding module and pulling out the plug of the test tube; the pipetting robot is used for sucking a sample and filling the sample into the culture dish. The steps from sample to culture can be completed at high speed and high efficiency, and the steps can be adjusted according to actual detection conditions through modular combination.

The invention provides a vascularized organ-like chip based on seal microstructure patterning and a preparation method thereof, the chip comprises a micro-channel layer, the upper surface of the micro-channel layer is provided with a gel perfusion channel, a central tissue chamber and culture medium flow channels at two sides which are connected, and tumor spheres or colon cells are inoculated in the central tissue chamber; the liquid storage pool layer is arranged on the upper surface of the micro-channel layer and corresponds to the inlet and outlet of the gel filling channel, the inlet and outlet of the central tissue chamber and the inlet and outlet of the culture medium runner, the liquid storage pool layer is provided with a plurality of liquid storage pools and a plurality of through holes, and the liquid storage pools contain culture solutions to provide nutrients for cultured tissues; and the seal module is arranged above the central tissue chamber, the seal module is provided with a tumor seal and a colon seal, and the tumor seal and the colon seal are respectively provided with a microstructure three-dimensional module and are respectively used for simulating the culture environment of tumor spheres or colon cells. According to the invention, in-vitro construction of the vascularized tumor model and the colon model is realized, and a guide is provided for construction of the vascularized organ-like model with specific organ three-dimensional microstructure characteristics.

The invention belongs to the technical field of biological material engineering, and particularly relates to a fungal imitation leather material and a preparation method thereof. The method comprises the following steps: firstly, adopting wild fomes fomentarius subjected to 60Co irradiation mutagenesis as a production strain of a mycelium pad, and screening and optimizing components and content of a liquid culture medium to obtain a surface culture liquid culture medium suitable for the wild fomes fomentarius; secondly, by utilizing the strain and an optimized culture medium, the low-cost and large-scale production of the mycelium pad can be realized under the optimal culture condition; and the mycelium pad is subjected to various physical and chemical treatments such as plasticizing, cross-linking, hot-pressing compaction and the like, so that the mechanical strength of the mycelium pad is further improved, and the mechanical property requirements of leather products are met.

The invention discloses a needle mushroom bacterial liquid culture reaction kettle, and relates to the technical field of bacterial liquid culture preparation equipment.The needle mushroom bacterial liquid culture reaction kettle comprises a rotating assembly, the rotating assembly is fixedly connected with a motor, the outer side of the rotating assembly is fixedly connected with a round pipe, square holes are formed in the outer side of the round pipe, and the square holes are evenly distributed with the round pipe as the center; a fixing ring is rotatably connected to the end, away from the rotating assembly, of the circular pipe, a rotating plate is fixedly connected to one end of the fixing ring, a supporting rod is fixedly connected to the end, close to the rotating plate, of the fixing ring, a reset spring is fixedly connected to the end, close to the circular pipe, of the supporting rod, and a baffle is slidably connected to the outer side of the supporting rod. According to the flammulina velutipes bacterial liquid culture reaction kettle, a culture solution and strains in the tank body are stirred in multiple degrees of freedom and different directions through rotation of the round pipe and the rotating plate, so that the strains and the culture solution are uniformly mixed, and the quality of culture production of the strains is ensured.

The invention discloses a closed mixotrophic microalgae culture method and a culture system thereof, and relates to the field of microalgae culture, comprising the following steps: step one, microalgae culture, pouring culture solution into a culture tank, adding microalgae to be cultured into the culture solution, and then fixing a sealing plate to the tank mouth of the culture tank by bolts to carry out closed culture; step two, gas supply and nutrient solution addition, sending carbon dioxide from a pipeline into an air distribution pipe through an air pump in an air supply component, forming bubbles from a nozzle and transporting them into the culture solution, and part of the bubbles that rise are gathered by a baffle to extend their residence time in a water body; step three, illumination, during the day, a first electric telescopic rod drives an arc-shaped telescopic plate to move downward to expose the internal transparent culture tank, and sunlight provides a light source to propagate the microalgae. The application can extend the residence time of carbon dioxide in the water body, improve the utilization rate of carbon dioxide, and increase the yield of microalgae.

The invention discloses a culture solution for reducing lipid of animal embryos before freezing as well as a preparation method and application of the culture solution. The culture solution is prepared by adding bezafibrate and cholic acid into an NCSU23 culture solution. The invention also discloses a preparation method of the culture solution, which comprises the following steps: preparing an NCSU23 culture solution, and adding bezafibrate and cholic acid into the NCSU23 culture solution. Compared with a conventional NCSU23 culture solution, the culture solution provided by the invention has the advantages that the added bezafibrate and cholic acid have a synergistic lipid-lowering effect, so that the content of lipid in an embryo can be remarkably reduced, and the survival rate and the blastocyst hatching rate after the embryo is frozen and unfrozen are finally effectively improved. The culture solution provided by the invention has application prospects in the aspects of animal embryo lipid reduction, frozen embryo survival and development and the like.

The invention discloses a method for improving generation of antagonistic substances of beneficial bacteria by utilizing bacteriophages. Under the continuous stress of the bacteriophage YL-Ste-01P, the yield of the bacteriostatic metabolic substances of the beneficial bacterium YL-Ste-01 is obviously increased. A bacterial wilt biocontrol bacterium YL-Ste-01 and a bacteriophage YL-Ste-01P are inoculated into an NA liquid culture medium for co-culture to obtain a culture solution, the culture solution is centrifuged to obtain supernate, and the supernate is filtered by a filter membrane to obtain fermentation liquor. According to the present invention, the co-culture fermentation broth of YL-Ste-01 and YL-Ste-01P, alpha-ketoglutaric acid, guanidinopropionic acid and fumagillin have significant inhibition effects on ralstonia solanacearum; the invention discloses application of an antagonistic substance in preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt. The invention discloses application of alpha-ketoglutaric acid, guanidinopropionic acid or fumagillin to preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt.

The invention discloses a method for screening and culturing active plastic degrading bacteria based on combination of Raman spectrum and heavy water labeling, which realizes rapid screening of high-activity plastic degrading bacteria within 7 days by utilizing intestinal microorganisms under the condition of inorganic salt culture incubation with plastic as a unique carbon source. The method comprises the following steps: dissecting and homogenizing intestinal tracts of tenebrio molitor fed with plastics under a sterile condition, harvesting enteric microorganisms through iohexol density gradient centrifugation, incubating the enteric microorganisms in an inorganic salt culture solution containing heavy water and plastics to serve as a treatment group, centrifugally cleaning an incubated to-be-detected substance, and performing Raman detection, the high-activity plastic degrading bacteria can be sorted for further downstream sequencing and culture. The method is rapid, efficient, easy and convenient to operate and suitable for rapidly screening, identifying and culturing the high-activity plastic degrading bacteria from a complex natural environment, and development of plastic biodegradation and harmless treatment processes is facilitated.

The utility model belongs to the technical field of mixing devices, and particularly relates to a culture solution mixing device for biological culture, which comprises a mixing kettle, the upper end of the mixing kettle is connected with a kettle cover through a screw, the upper end of the kettle cover is fixedly connected with a hopper, a feeding mechanism is arranged on the hopper, and a motor is fixedly mounted in the middle of the upper end of the kettle cover. A rotating shaft of the motor penetrates through the kettle cover and is rotationally connected with the kettle cover, a main shaft is welded to the lower end of the rotating shaft, a cleaning mechanism is arranged on the outer side of the main shaft, and a scraping plate is arranged on the cleaning mechanism. The main shaft drives the supporting disc to rotate, water is fed into the supporting disc through the water pump, water pressure enables the hollowed-out sliding pipe to move to extrude the spring, the holes in the hollowed-out sliding pipe correspond to the holes in the hollowed-out square pipe in position, water is sprayed into the mixing kettle, and the interior of the mixing kettle is cleaned more conveniently through scraping of the scraping plate.

The utility model relates to the technical field of cell culture, and discloses a cell culture bottle, comprising a culture bottle, wherein a bottom wall is arranged at the bottom of the culture bottle, a liquid-blocking and breathable membrane is arranged at the opening of the culture bottle, a closing cover is installed on the outside of the culture bottle, a fixing ring is fixedly connected to the outside of the culture bottle, a plug is arranged inside the fixing ring, and a sealing block is fixedly connected to the inside of the plug. The cell culture bottle has the advantages of being convenient for adding culture fluid and reducing the influence of the external environment on the internal environment of the culture bottle. The plug is used to close a pipeline, and the culture bottle is divided into two cavities by a partition plate, so that culture fluid can be added through the pipeline, and a blocking mechanism is opened by gravity to add culture fluid to the inside of the cavity for culturing cells, so as to complete the addition of culture fluid, thereby solving the problem that the external environment may contaminate cells when the closing cover is opened to pour the culture fluid through the opening during the current cell culture process.

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention provides a constant-turbidity continuous culture device which is used for culturing photosynthetic organisms, and the constant-turbidity continuous culture device comprises a mounting main body which comprises a shell, an input pipe and an output pipe, and the input pipe and the output pipe are connected to the shell; the input pump is communicated with the input pipe; the output pump is communicated with the output pipe; the optical sensor is connected to the mounting main body and is used for detecting the growth information of the photosynthetic organisms in real time; and the control circuit is electrically connected with the input pump, the output pump and the optical sensor and is used for controlling the input pump to input a culture medium through the input pipe and / or controlling the output pump to output a photosynthetic organism culture solution through the output pipe according to the growth information. The invention further provides a constant-turbidity continuous culture method.

The invention discloses a submerged plant culture and root exudates circulating collection device, and relates to the technical field of exudates collection, the submerged plant culture and root exudates circulating collection device comprises a culture box, the bottom of the culture box is fixedly connected with a plurality of connecting rods, the ends, away from the culture box, of the connecting rods are fixedly connected with a collection box, and the side, away from the connecting rods, of the culture box is movably connected with a top cover; a lamp is fixedly connected to the side, close to the incubator, of the top cover, a plurality of culture fixing pipes are movably connected to the interior of the incubator, a secretion collecting assembly is arranged on the incubator, and the secretion collecting assembly comprises a threaded rod and an extrusion rod which are rotationally connected to the interior of the incubator, and a spring and an inclined block which are symmetrically and movably connected to the interior of the incubator; the threaded rod rotates to drive the extrusion rod to rotate, the extrusion rod rotates around the special inclined block in a sliding mode, root exudates can be more effectively released into a culture solution through the exudates collecting assembly, and therefore the exudates collecting efficiency is improved.

The utility model relates to the field of culture frames, and discloses a seedling planting culture frame which comprises a water storage tank, a chassis is fixedly connected to one side of the upper surface of the water storage tank, and a motor is fixedly connected to the center of the lower surface of the water storage tank; according to the device, the motor is connected with the stirring paddle, so that the stirring paddle is driven by the motor to rotate, water in the water storage tank and a culture solution are fully stirred and mixed by the stirring paddle, and then the spray head is communicated with the water storage tank through the hose; a water pump sprays liquid in a water storage tank from the position of a spray head through hoses on the two sides, redundant culture liquid flows out of a water outlet in the bottom of a culture cylinder and flows into a base plate to be stored, then the redundant culture liquid flows into the water storage tank from the position of a through hole, spraying on nursery stocks again is facilitated, and resource waste is reduced; the effects of conveniently spraying the culture solution to the seedlings and collecting and utilizing the redundant culture solution are achieved.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.

The invention belongs to the technical field of mushroom cultivation, and discloses an earthing cultivation mushroom cultivation box which is used as an independent unit for fermentation, mushroom cultivation and fruiting of earthing cultivation mushrooms and comprises an open box body and an aeration layer arranged at the bottom of the box body, and an inner air channel and an inner water channel are embedded in the box body. The inner air channel is provided with an aeration port in the aeration layer in the box body, gas entering the aeration layer from the aeration port enters a culture medium covering the aeration layer from an opening in the surface of the aeration layer, and the inner water channel is provided with an inner nozzle for spraying a culture solution into the box body at the upper part of the culture medium in the box body. The movable platform is used for bearing a plurality of box bodies to form a culture module, and a main air passage and a main water passage are arranged at the lower part of the movable platform and are used for supplying air and water to each opposite box body. The cost is reduced.

The present invention relates to the technical field of Haematococcus pluvialis cultivation, and specifically relates to a disinfection device for a Haematococcus pluvialis cultivation system, which includes an algae storage tank, a disinfection tank, and a number of cultivation tubes. A separation part is connected and arranged between two adjacent upper and lower ones among a number of cultivation tubes on the same side. By starting the double-headed motor to drive the partition to rotate 90 degrees, a number of liquid passing boxes and connecting tubes are blocked, so as to cut off two adjacent cultivation tubes at the same height, and at the same time, the two adjacent upper and lower cultivation tubes are connected to form a loop path. Then, the disinfectant with a large density sinks into the lower cultivation tube to form a layer, and it can be pumped and drained, while the Haematococcus pluvialis culture solution is retained in the cultivation tube for continuous cultivation. Then, just open the liquid outlet pipe to release the disinfectant. Therefore, it is not necessary to lead out and collect all the Haematococcus pluvialis culture solution and then separate the disinfectant. This device saves the time and process of disinfection and separation, and avoids the long-term adverse effects of the disinfectant on the Haematococcus pluvialis culture solution.

Provided is a photobioreactor device for microalgae culture, characterized in that the device comprises: an outer container for containing a culture solution, the outer container comprising a transparent side wall and a removable upper cover; the inner container is used for containing culture liquid and microalgae and can be contained in the outer container and taken out of the outer container, the inner container comprises a transparent side wall, a top screen and a bottom screen, each of the top screen and the bottom screen comprises a screen mesh, the mesh size of the screen mesh can allow the culture liquid and gas to pass through, and meanwhile the microalgae is prevented from passing through; at least one of the top screen and the bottom screen is removably engageable with the inner container; and the gas circulation unit injects gas rich in carbon dioxide into the outer container.

The utility model discloses a three-dimensional rotary replaceable culture solution cell microgravity device, which comprises a bracket and a square frame, a cell culture solution shell is arranged at the top of the square frame, a cell replacement solution shell is arranged at the bottom of the square frame, a replacement solution inlet is arranged at the bottom of the cell culture solution shell, and a cell culture solution is arranged in the replacement solution inlet. A replacement liquid outlet is formed in the top of the replacement liquid cavity, a liquid outlet is formed in the top of the cell culture liquid shell, and a liquid inlet is formed in the top of the liquid outlet cavity. The check valve I, the check valve II and the pressure pump are respectively arranged on the surfaces of the replacement pipeline and the discharge pipeline, so that the problem of liquid backflow in the rotating process of the square frame is avoided; and meanwhile, the replaced liquid can be stored in the liquid discharging cavity no matter the cell culture liquid shell is at any inclined angle in the liquid replacement process, and the condition of cross contamination of the culture liquid in the cell culture liquid shell is avoided.

The aim of the present invention is to provide a plant culturing apparatus and a plant culturing method for a plant whole body that allow liquid phase roots and vapor phase roots to separately appear in the root system thereof without using soil. More specifically, according to the present invention, the above described aim is achieved by culturing a plant whole body in the plant culturing apparatus for a plant whole body, which comprises a light-shading casing that is provided with at least one through hole on the ceiling surface and holds a culture liquid, and an inner surface showing capillary force, that are arranged on at least a part of an inner side surface from among an inner surface of the light-shading casing.

The invention relates to a method for electrically activating oocytes based on nanosecond pulses. Single oocytes are sequentially and uniformly arranged between two parallel electrodes of an electrode dish, the electrode dish is characterized in that the distance between the electrodes is 1 mm, the electrodes are placed in a culture dish, and the two ends of the two electrodes are sealed; a culture solution is firstly put between the electrodes which are arranged in parallel, mineral oil is covered to prevent the culture solution from being evaporated, after preheating treatment, oocytes are sequentially and uniformly put between the two electrodes which are arranged in parallel one by one, and then nanosecond pulse electric activation treatment is applied. The electrodes which are arranged in parallel are adopted, the oocytes are sequentially and uniformly placed between the electrodes in a single mode, the situation that the electric pulse effect is reduced due to adhesion and stacking of all the cells is avoided, the oocytes receive uniform and equivalent electric pulse sequences, the action effect and stability of electric pulses on the cells are obviously improved, and the operation efficiency of the oocytes is improved. And the reliability and reproducibility of a test conclusion are improved.

The invention discloses a biological reaction device and a stirring method, and relates to the technical field of bioreactors, the biological reaction device comprises a container, an execution device, a sensor and a control device, the execution device comprises a base and a plurality of stirring paddles arranged on the base, the base is detachably installed on the container, the stirring paddles can be inserted into the container, and the sensor is arranged on the container. Each stirring paddle comprises a rotating shaft and a plurality of paddles, the paddles are inclined to the horizontal plane, the rotating directions of every two adjacent stirring paddles are opposite, liquid in the containing cavity can flow along the rotating axis of the stirring paddles in the rotating process of the stirring paddles, and the mixing effect of the cells and the culture solution is improved. The sensor is inserted into the channel of the rotating shaft and extends out of the stirring paddle, parameters in a culture solution can be detected in real time, and the control device can receive a detection signal of the sensor and control the rotating speed of the stirring paddle based on the detection signal, so that full contact and reaction of cells and the culture solution are guaranteed, and the biological culture effect is improved.

The invention belongs to the technical field of microorganisms, and discloses a method for optimizing thalli by using alpha-ketoglutaric acid and producing inositol by fermentation, which comprises the following steps: carrying out gradient dilution on the thalli containing inositol monophosphate by using sterile water, then inoculating to a selective culture medium containing alpha-ketoglutaric acid, and culturing to obtain the inositol by using the alpha-ketoglutaric acid. The method comprises the following steps of: selecting uniform and single bacterial colonies with raised bacterial colonies and bacterial colony diameter of 2-3mm, transferring the bacterial colonies to a plate culture medium, shaking a flask and carrying out fermentation culture, regulating fermentation conditions by adding arabinose and sodium carbonate, and finally detecting the content of inositol by using HPLC (High Performance Liquid Chromatography) to screen. The method is simple, the high-yield strain screening efficiency is high, the obtained strain is high in stability, and the yield of inositol produced through fermentation is high.

The invention discloses a bile conversion product and a preparation method thereof, and belongs to the field of bioengineering technology. The preparation method comprises the following steps: mixing the culture fluid of 7α hydroxysteroid dehydrogenase and 7β hydroxysteroid dehydrogenase secreted and expressed by engineering bacteria with a buffer solution after adsorption, impurity removal, and ultrafiltration concentration, and adding the culture fluid to an ion exchange agar gel column to immobilize the enzyme after adjusting the pH, thereby obtaining an immobilized enzyme column; mixing the avian bile extract with NADP+, adjusting the pH, and then adding the mixture to the immobilized enzyme column for conversion, and obtaining the bile conversion product after the clear liquid is concentrated under reduced pressure and dried under vacuum. The invention uses an ion exchange agar gel column as a carrier for immobilizing hydroxysteroid dehydrogenase, which has good stability, high mechanical strength, and large loading capacity, and the enzyme molecules are not easy to fall off, and can react continuously and the reaction product can be separated from the enzyme in time, thereby reducing the occurrence of reverse reaction, thereby obtaining a bile conversion product with a higher ratio of TUDCA:TCDCA.

The invention relates to the technical field of agricultural seedling raising, and discloses a seed hydroponic germination incubator for agricultural seedling raising, which comprises an incubator body and a plurality of cultivated seeds placed in the incubator body, and is characterized in that a supporting plate used for bearing the cultivated seeds is arranged in the incubator body, an annular sliding frame is arranged on the outer side wall of the supporting plate, and the annular sliding frame is arranged on the outer side wall of the supporting plate. The annular sliding frame is connected with a floating plate through a connecting rod, the side wall of the incubator body is provided with a liquid supplementing pipe for supplementing and replacing the culture solution into the incubator body, and the liquid level of the culture solution in the incubator body is a liquid level line. The length of the pushing assembly is the same as that of the connecting rod, so that it can be guaranteed that in the lifting and rising process of the pushing assembly, cultivated seeds located above the pushing assembly can be pushed to rise together and are just located at the liquid level line position, and the cultivated seeds are located at the liquid level line position; therefore, the germinated cultivated species are pushed out of the supporting plate to reach the liquid level line, and the environment where the germinated cultivated species are located is changed.

The utility model discloses a double-sleeve continuous flushing negative pressure aspiration liquid culture medium set, which comprises an aerobic culture bottle, an anaerobic culture bottle and a culture bottle placing box, the aerobic culture bottle and the anaerobic culture bottle are arranged in the culture bottle placing box, an air cleaning component and an injection component are arranged in the anaerobic culture bottle, drainage liquid is stored in the injection component, and the drainage liquid is filled in the culture bottle placing box. And the air removal assembly exhausts air in the drainage liquid in the injection assembly. The device has the advantages that culture flasks containing aerobic and anaerobic culture solutions are stored in the culture flask placement boxes, the culture flasks can be opened for use during sampling, the negative pressure connector and the liquid inlet connector are directly connected with the negative pressure tube and the double sleeves, negative pressure sampling of drainage liquid is achieved, operation can be achieved by a single person, medical staff are prevented from making contact with the drainage liquid, and the air removal assembly is used for removing air from the drainage liquid. The air in the drainage liquid in the injection tube is discharged, and then the drainage liquid with the air removed is injected into the anaerobic culture bottle through the injection assembly, so that the air is prevented from entering the anaerobic culture liquid, and the accuracy of a culture result is ensured.

The invention discloses a multi-layer uniform mixing method for microorganism culture, which comprises the following steps: putting: putting microorganisms to be cultured and a culture solution into test tubes, and putting the test tubes into a plurality of culture cavities arranged in a culture box layer by layer in batches; temperature regulation: independently regulating and controlling the temperature in the plurality of culture cavities by a temperature regulation and control mechanism; uniformly mixing: the test tubes revolve and rotate in the incubator, and meanwhile, the test tubes are pushed to oscillate in a small amplitude under the action of an oscillation mechanism, so that the microorganisms and the culture solution are uniformly mixed. The incubator is provided with a plurality of culture cavities, bin doors are hinged to the front sides of the culture cavities, the incubator comprises trays, rotating discs, placement grooves, oscillation blocks and a temperature regulation and control mechanism, the trays are installed in the culture cavities in a sliding mode, the rotating discs are rotatably arranged at the tops of the trays, and the placement grooves rotate while rotating along with revolution of the rotating discs. The invention is used for solving the problem of inaccurate detection result caused by poor mixing effect of the existing mixing device.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The utility model discloses a root exudate collecting device which comprises a culture tube, a partition plate is detachably connected into the culture tube, a positioning hole is formed in the partition plate, and cavities, located on the upper side and the lower side of the partition plate, in the culture tube are filled with glass beads respectively; an extraction assembly is installed on the extraction tank, the input end of the extraction assembly is fixedly communicated with the bottom end of the culture tube, and the output end of the extraction assembly is fixedly communicated with the side wall of the top end of the extraction tank; the cleaning assembly is mounted in the extraction tank; the filter assembly comprises a liquid discharge pipe fixedly connected to the bottom of the extraction tank, one end of the liquid discharge pipe is fixedly communicated with a water inlet of the infusion pump, and a filter is mounted on the liquid discharge pipe; and the output end of the infusion pump is fixedly communicated with the storage box through a conveying pipe. The glass beads are used for replacing soil to cultivate plants, culture liquid is directly discharged through the bottoms of the culture tubes during extraction, plant root systems cannot be affected, and damage to the plant root systems is reduced.