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1264 results about "Culture fluid" patented technology

Culture fluid. a fluid in which microscopic organisms are made to develop, either for purposes of study or as a means of modifying their virulence. If the fluid is gelled by, for example, the use of agar, it then is called, depending on the vessel in which the gelled medium is contained, a plate, a slant, or a stab.

Manufacturing process for the production of polypeptides expressed in insect cell-lines

The present invention provides a manufacturing method for polypeptides that are produced in insect cells using a baculoviral expression system. In one example, the insect cell culture is supplemented with a lipid mixture immediately prior to infection (e.g., one hour prior to infection). The polypeptides are isolated from the insect cell culture using a method that employs anion exchange or mixed-mode chromatography early in the purification process. This process step is useful to remove insect-cell derived endoglycanases and proteases and thus reduces the loss of desired polypeptide due to enzymatic degradation. In another example, mixed-mode chromatography is combined with dye-ligand affinity chromatography in a continuous-flow manner to allow for rapid processing of the insect-cell culture liquid and capture of the polypeptide. In yet another example, a polypeptide is isolated from an insect cell culture liquid using a process that combines hollow fiber filtration, mixed-mode chromatography and dye-ligand affinity in a single unit operation producing a polypeptide solution that is essentially free of endoglycanase and proteolytic activities. In a further example, the isolated polypeptides are glycopeptides having an insect specific glycosylation pattern, which are optionally conjugated to a modifying group, such as a polymer (e.g., PEG) using a glycosyltransferase and a modified nucleotide sugar.
Owner:NOVO NORDISK AS

Bacillus amyloliquefaciens growing in disease-preventing and growth-promoting plant and application thereof

ActiveCN101948771AHas development and application valueImprove efficiencyBiocideBacteriaDiseaseCulture fluid
The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.
Owner:INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI

Meat chick granulated feed containing active bacterium fermentation ingredient and traditional Chinese medicine ingredient

The invention discloses a meat chick granulated feed containing an active bacterium fermentation ingredient and a traditional Chinese medicine ingredient. The meat chick granulated feed comprises 5-15 percent of the active bacterium fermentation ingredient, 0.3-1 percent of the traditional Chinese medicine ingredient and the balance of a full value chicken feed. The active bacterium fermentation ingredient is prepared by adopting the following method of: adding an aspergillus niger culture solution, a probiotics culture solution and a yeast culture solution to a fermentation medium respectively according to an inoculation quantity of 5-15 percent for fermentation comprising primary anaerobic fermentation and secondary aerobic fermentation; and after the fermentation, carrying out low-temperature drying at 55 DEG C and smashing on the culture solution to obtain the active bacterium fermentation ingredient. Proved through tests, the feed provided by the invention can be used for obviously improving the food consumption and the daily gain of meat chicken and reducing the feed conversion ratio and the diarrhea rate, does not contain antibiotics, has no toxic and side effects and environmental pollution and can be used for providing high-quality green livestock and poultry products.
Owner:孙萍

Soilless vegetable cultivation machine

The invention relates to a soilless vegetable cultivation machine which comprises a cultivation rack and a constant-temperature tank. A plurality of vertically arrayed cultivation layers are arranged in the cultivation rack and are sequentially connected with one another by drain pipes, a culture fluid tank is communicated with a spray pipe in the uppermost cultivation layer via a suction pipe, and a lowermost culture fluid groove is communicated with the culture fluid tank via a drain pipe; the constant-temperature tank is arranged at the bottom of the cultivation rack, and a temperature control assembly b and a temperature sensor b are arranged inside the constant-temperature tank; the culture fluid tank and a seedling raising assembly are arranged inside the constant-temperature tank, and a filter box is further arranged in the culture fluid tank; the constant-temperature tank is communicated with a temperature control chamber via an air channel, and the temperature control chamber is positioned on the top of the cultivation rack; an air outlet is formed in a side wall of each cultivation layer and is connected with a pipe of the air channel. The soilless vegetable cultivation machine has the advantages that the soilless vegetable cultivation machine is applicable to domestic and small-scale vegetable cultivation and vegetable production of indoor medium and large plant factories and is low-nitrate, green and healthful vegetable production equipment free of pesticides, hormone and heavy metal residues.
Owner:汪蕾

Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid

The invention discloses a method for repairing cement-based material cracks, as well as a culture solution and a repair nutrient solution. The method for repairing cement-based material cracks by through microorganisms comprises the following steps that: a Bacillus pasteurii strain is inoculate onto a culture medium provided with a urea-containing substrate; shake cultivation is carried out at a temperature of between 25 and 37 DEG C, and then a culture bacteria solution is taken out and centrifuged and has supernatant fluid removed; strain cells are collected through the culture solution; the concentration of the strain cells is controlled in a range of between 2x10<9> and 1x10<11> cell/ml; standard sand, urea and Ca(NO3)2.4H2O mixture are added to each milliliter of strain cell solution obtained through collection, mixed, stirred into slurry and injected into cement stone cracks; the frequency of the repair nutrient solution injection is not less than two times; finally, maintenance is carried out. In the culture solution, each liter of culture solution contains 4 to 6 g of peptone, 2 to 4 g of beef extract and 20 to 60 g of urea. The method fully utilizes microbial resources in nature; CO3<2-> decomposed out through microbial enzyme can chelate Ca<2+> in a substrate so as to be mineralized and deposit calcium carbonate, and is close in the combination with the substrate and good in stability.
Owner:SOUTHEAST UNIV

Method for cultivating nitrosobacteria flora and method for treating wastwater containing ammonia nitrogen

ActiveCN101434915AImproving the Efficiency of Removing Ammonia Nitrogen in Biochemical TreatmentSimple processBacteriaWater contaminantsHigh concentrationCulture fluid
The invention discloses a culture method of a nitrosomonas sp. flora and a treatment method of waste water containing ammonia nitrogen. The ammonia nitrogen concentration and the pH value of a matrix are increased during an enrichment process, and free NH3 with higher concentration is maintained, thereby gradually achieving the purposes of eliminating nitrobacter sp. and enriching nitrosomonas sp. and obtaining a nitrosomonas sp. flora. Then, the obtained nitrosomonas sp. flora is taken as seeds, the expanded culture is carried out by utilizing self-prepared ammonia nitrogen waste water or high ammonia nitrogen waste water as a culture liquid, the nitrosomonas sp. flora after the expanded culture is inoculated into a biochemical treatment pool for improving the removal efficiency of ammonia nitrogen by biochemical treatment, or the nitrosomonas sp. flora after the expanded culture is directly used for removing the ammonia nitrogen of the ammonia nitrogen waste water, and the optimal proposal is used in the aerobic nitrification stage of the shortcut nitrification-denitrification process for accumulating nitrite nitrogen as an electron acceptor for denitrification directly. The method has simple process and outstanding effect of treating the waste water containing the ammonia nitrogen.
Owner:CHINA PETROLEUM & CHEM CORP +1

Preparation method of graphene/bacterial cellulose composite material

The invention discloses a preparation method of a graphene/bacterial cellulose composite material. Bacterial cellulose and graphene are co-cultured in situ to obtain the graphene/bacterial cellulose composite material, wherein the graphene evenly grows in the bacterial cellulose reticular fiber structure. The preparation method comprises the following steps: 1) preparing a bacterial cellulose culture fluid, and sterilizing at high temperature under high pressure for 30-60 minutes; 2) inoculating the strain in the bacterial cellulose culture fluid, and rocking in a rocker for 12-48 hours; 3) carrying out ultrasonic treatment on a 0.2mg/ml graphene dispersion liquid for 1-3 hours, and adding the graphene dispersion liquid into the bacterial cellulose culture fluid with the strain, wherein the volume ratio of the graphene dispersion liquid to the bacterial cellulose culture fluid is 1:5-1:10; 4) rocking the mixed liquid in the rocker for 12-24 hours; 5) putting the liquid in a 28 DEG C thermostatic oven, and standing for 1-2 weeks to obtain the graphene/bacterial cellulose composite material; and 6) cleaning the graphene/bacterial cellulose composite material, and carrying out freeze-drying. In the composite material, the graphene is evenly distributed on the bacterial cellulose fibers, thereby effectively inhibiting the defect of high agglomeration tendency of graphene particles.
Owner:EAST CHINA JIAOTONG UNIVERSITY

CIK cell, as well as preparation method and cell preparation thereof

The invention discloses a CIK cell, as well as a preparation method and a cell preparation thereof. The method for preparing the CIK cell comprises the following steps: placing a separated mononuclear cell in a culture fluid containing phytohemagglutinin; transplanting the mononuclear cell into a culture flask enveloped by antiCD3 monoclonal antibody and antiCD28 monoclonal antibody after the cell is cultured for 24 to 72 hours; and adding a culture fluid containing IL-1alpha and IL-2 into the culture flask to keep on culturing for 5 to 15 days, and separating the cells into different flasks to be cultured every 2 to 3 days. The CIK cell prepared by the method has the characteristics of obvious improved cell proliferation, great increase of CD8 cell proportion, wide antineoplastic spectrum and strengthened antineoplastic activity. The CIK cell and the cell preparation can effectively prevent the metastasis and the recrudescence for of postoperative patients with tumor, can be combined with chemicotherapy to effectively reduce toxic and side effects of the chemicotherapy, strengthens the survivability tolerance of the patients to improve healing efficacy, and can obviously prolong lifecycle to of end-stage patients so as to improve the life living quality.
Owner:上海德嘉生物科技有限公司 +1

Space plant culture device

The invention provides a space plant culture device comprising a culture box which is partitioned into a left chamber and a right chamber via a longitudinal partition. The left chamber is provided with an upper layer and a lower layer, an azolla moist cultivation tray is arranged on the upper layer, a vegetable moist cultivation tray is arranged on the lower layer, the right chamber is provided with a seedling cultivation tray, an LED lamp panel is arranged above the azolla moist cultivation tray, the vegetable moist cultivation tray and the seedling cultivation tray, and the right chamber is further provided with a bag-type solution collecting box used for providing a culture solution and a peristaltic pump used for driving the culture solution to flow. The space plant culture device is provided with a plant culture solution supply system, an LED illumination system and a monitoring system which are complete and needed for growth of azolla and short small salad vegetables in the whole growth period, on-line parameter setting, automatic record storing and intelligent system controlling can be realized, population, individual photosynthetic efficiency and biological-yield efficient production cycle of the plants as well as change rules in space microgravity environment of the plants can be researched, and requirements on spatial scientific experiments and space station key technology experiments can be met.
Owner:AGRI ECOLOGY INST FUJIAN ACADEMY OF AGRI SCI

Method by utilizing double markers to acquire share of inorganic carbon source utilized by plants

The invention discloses a method by utilizing double markers to acquire share of inorganic carbon source utilized by plants, which comprises the following steps that: A. sodium bicarbonate produced by different manufacturers is determined, two types of sodium bicarbonate with delta 13C difference being more than eight thousandths are used as an isotopic marker 1 and an isotopic marker 2 to be respectively added into nutrient fluid to culture plants; delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 1 is delta C1, and delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 2 is delta C2; and B. after more than four leaves appear, the values of delta 13C consisting of stable carbon isotopes of plant leaves to be observed correspondently culturedby the nutrient fluid of the two types of isotopic markers are respectively determined as delta T1 and delta T2; and C. delta C1, delta C2, delta T1 and delta T2 are brought into equation fB= to calculate the share fB of the carbonate ions utilized by the plants. Due to the adoption of the method, the share of the inorganic carbon source utilized by the plants can be rapidly acquired, the steps are fewer, simplicity in calculation can be realized, and data is reliable.
Owner:INST OF GEOCHEM CHINESE ACADEMY OF SCI

Production method of agricultural photosynthetic bacteria preparation

The invention relates to a production method of an agricultural photosynthetic bacteria preparation, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35 DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heat disinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the agricultural photosynthetic bacteria preparation under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r/m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the preparation has the advantages of stable product quality and diversified functions.
Owner:SHUNDE HONGLONG BIOLOGY SCI & TECH

Biochemical treatment process for high-concentration ammonia-nitrogen-containing waste water

The invention discloses a biochemical treatment method for wastewater with high ammonia nitrogen concentration. The method is as follows: nitrobacterium-enriched activated sludge is cultured in a nitrified sludge enrichment tank at first and then is led into a nitration treatment tank containing wastewater with high ammonia nitrogen content and low COD, and sewage after nitration treatment enters into a denitrification tank for nitrification and denitrogenation treatment, wherein, the process for culturing the nitrobacterium-enriched activated sludge adopts the intermittent activated sludge process and enrichment is performed by gradual improvement of the concentration of ammonia nitrogen in culture solution; the used enrichment culture solution comprises microelements including ferrum, magnesium, sodium and potassium and buffer solution, and particularly also comprises Ca<2+> with a concentration between 0.01 and 0.05 gram per liter; the final concentration of the ammonia nitrogen during the culture process is between 300 and 1200 micrograms per liter; and the COD is less than or equal to 200 micrograms per liter. The method can effectively process the wastewater with high ammonia nitrogen content and low COD value, and the processing method is simple and low in cost.
Owner:CHINA PETROLEUM & CHEM CORP +1
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