172 results about "Culture fluid" patented technology

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention discloses a multi-layer uniform mixing method for microorganism culture, which comprises the following steps: putting: putting microorganisms to be cultured and a culture solution into test tubes, and putting the test tubes into a plurality of culture cavities arranged in a culture box layer by layer in batches; temperature regulation: independently regulating and controlling the temperature in the plurality of culture cavities by a temperature regulation and control mechanism; uniformly mixing: the test tubes revolve and rotate in the incubator, and meanwhile, the test tubes are pushed to oscillate in a small amplitude under the action of an oscillation mechanism, so that the microorganisms and the culture solution are uniformly mixed. The incubator is provided with a plurality of culture cavities, bin doors are hinged to the front sides of the culture cavities, the incubator comprises trays, rotating discs, placement grooves, oscillation blocks and a temperature regulation and control mechanism, the trays are installed in the culture cavities in a sliding mode, the rotating discs are rotatably arranged at the tops of the trays, and the placement grooves rotate while rotating along with revolution of the rotating discs. The invention is used for solving the problem of inaccurate detection result caused by poor mixing effect of the existing mixing device.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The utility model belongs to the technical field of microbial agent culture, and particularly relates to a microbial agent improved saline-alkali soil culture device which comprises a culture box and a shaking assembly arranged in the culture box, the shaking assembly comprises a rotating disc, a transmission rod, a supporting plate, a stabilizing frame, a lap joint frame and a layered net frame, a transmission groove is formed in the bottom face of the transmission rod, and the supporting plate is arranged in the transmission groove. And the supporting plate is located on the upper side of the transmission rod, a rolling contact rolling wheel is arranged on the bottom face of the supporting plate, and the rolling wheel is rotationally installed on the upper side of the transmission rod. The culture dishes in the culture box can be shaken in different states through the shaking assembly, so that a culture solution is always kept in a dynamic mixing state, the dynamic environment of the saline-alkali soil is simulated, precipitation of saline-alkali components is avoided, the activity and reproduction efficiency of a microbial agent are improved, and the improvement effect of the microbial agent on the saline-alkali soil is enhanced.

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

A plurality of liquid storage bags and waste liquid bags are arranged in a liquid storage area, a liquid separation chip and a plurality of peristaltic pumps and electromagnetic valves are arranged in a liquid separation area, four reaction chips are arranged in a reaction area, each reaction chip can support two parallel experiments, and the different areas are connected through hoses. A culture solution in the liquid storage area is controlled by a pump valve and a liquid separation chip in the liquid separation area, flows into different reaction chips, is subjected to microbial culture and interaction experiments with materials, and finally flows into a waste liquid bag. The reaction chip can be used for collecting electrochemical signals, and real-time observation can be carried out through a microscope. The chip card box provided by the utility model is designed to be totally closed, is high in integration level, can support simultaneous operation of a plurality of reactions, and is safe and reliable.

The invention discloses an organoid culture monitoring device which comprises a culture layer and a plurality of sensing modules, the culture layer is provided with a plurality of culture grooves used for culturing organoid, and the culture grooves are filled with conductive culture liquid. Each sensing module is arranged in one culture tank, and the sensing end of the sensing module is submerged in the culture solution and is used for monitoring the growth condition of the organoids in the culture tanks; the sensing module comprises a first insulating substrate and a detection unit, the first insulating substrate is attached to the groove wall of the culture groove in a matched mode so as to surround the organoid located in the culture groove or cells used for culturing the organoid, and the detection unit is arranged on the side, facing the organoid or the cells, of the first insulating substrate; and a groove-shaped sensing interface surrounding the cells or the organoids is formed in the culture groove. According to the technical scheme, the groove-shaped sensing interface is formed, so that non-contact, non-destructive, in-situ and long-time-history synchronous monitoring of the organoids is effectively realized.

The utility model discloses an escherichia coli culture device capable of supplementing materials for multiple times. The Escherichia coli culture device capable of supplementing materials for multiple times comprises a culture dish box and a culture dish cover, an isolating inner ring cylinder is integrally mounted in the culture dish box, an overflow port is formed in the isolating inner ring cylinder, and a first isolating plate and a second isolating plate are integrally mounted on the isolating inner ring cylinder and the inner wall of the culture dish box. According to the Escherichia coli culture device capable of supplementing materials for multiple times provided by the utility model, the isolation inner ring cylinder is arranged in the culture dish box, and the first isolation plate and the second isolation plate are arranged between the culture dish box and the isolation inner ring cylinder, so that a liquid discharge cavity is formed among the isolation inner ring cylinder, the first isolation plate, the culture dish box and the second isolation plate; and the bottom of the liquid discharge cavity is arranged to be an inclined plane, so that the discharged culture liquid can be automatically and continuously collected towards the liquid extraction tank, and the residue of the waste culture liquid can be reduced as much as possible.

To provide a culture apparatus in which microalgae do not accumulate on the inner bottom surface of the containment section. [Solution] The culture apparatus 10 comprises a containment section 20 capable of containing culture solution CS and microalgae, a guide section 40 for guiding culture gas G, and a gas supply section 30 for supplying culture gas G to the culture solution CS. The supply pipe section 36 constituting the gas supply section 30 has a main pipe section 362 extending along the depth direction of the containment section 20 and a sub-pipe section 363 extending along a protruding direction intersecting the depth direction. The outlet 38 formed in the sub-pipe section 363 is located below the lower end 43 of the guide section and is formed in a portion of the sub-pipe section 363 that protrudes in the protruding direction from the lower end 43 of the guide section.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The application relates to the technical field of microbial fermentation, and discloses a jet stirring device for microbial fermentation and a fermentation tank thereof. The jet stirring device comprises a stirring mechanism, and the stirring mechanism comprises a stirring main shaft. The bottom outer side of the stirring main shaft is provided with a first stirring structure capable of rotating and stirring the fermentation substrate and lifting the fermentation substrate. The middle outer side of the stirring main shaft is provided with a second stirring structure matched with the first stirring structure and capable of disturbing the flow of the lifted fermentation substrate. In the application, the opposite jet flow design (opposite rotating directions but periodic synchronization) of the first jet flow hole and the second jet flow hole is adopted to realize the forced collision and mixing of the fermentation bottom liquid and the culture liquid. Compared with the mixing mode of the prior art which only depends on the mechanical rotation of the stirring blade and the swinging of the tank body, the stability of the tank body is ensured, and the fermentation efficiency is improved.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The present application is an algal culture device for culturing algae by flowing a culture solution, the algal culture device comprising: a columnar carrier having at least an outer surface of a porous material; a carrier rotating mechanism for rotating the columnar carrier around a central axis in the length direction; a culture solution receiving portion provided below the columnar carrier; a culture solution supply mechanism for supplying the culture solution to the columnar carrier; and a culture solution circulating mechanism for circulating the culture solution accumulated in the culture solution receiving portion to the culture solution supply mechanism. A scraper can be further provided, which is configured to work along the outer surface of the columnar carrier in a manner to recover algae generated on the outer surface of the columnar carrier. The porous material can be ceramic at least in part. The columnar carrier can be cylindrical at least in part. The columnar carrier can be tubular at least in part.

The aim of the present invention is to provide a plant culturing apparatus and a plant culturing method for a plant whole body that allow liquid phase roots and vapor phase roots to separately appear in the root system thereof without using soil. More specifically, according to the present invention, the above described aim is achieved by culturing a plant whole body in the plant culturing apparatus for a plant whole body, which comprises a light-shading casing that is provided with at least one through hole on the ceiling surface and holds a culture liquid, and an inner surface showing capillary force, that are arranged on at least a part of an inner side surface from among an inner surface of the light-shading casing.

The utility model discloses a novel microalgae air purification oxygenation device which comprises a shell, a modular filter chamber and a microalgae incubator, wherein the modular filter chamber and the microalgae incubator are arranged in the shell; the shell is provided with an air outlet and an air inlet; the modular filter chamber comprises a plurality of filter layers and a photocatalyst coating purification chamber; the microalgae culture device comprises a microalgae culture solution water tank, a fan case, an aeration head and a liquid discharge pipe, the bottom of the microalgae culture solution water tank is sealed, and the top of the microalgae culture solution water tank is communicated with the air outlet; a light source is arranged at the bottom of the microalgae culture solution tank; the fan case is provided with a centrifugal fan, the aeration head is arranged in the microalgae culture solution water tank and is communicated with the fan case through an air pipe, and the fan case is communicated with the photocatalyst coating purification chamber; the bottom of the microalgae culture solution water tank is communicated with a liquid outlet formed in the lower part of the shell through a liquid outlet pipe. The internal structure is optimized, microalgae culture, microalgae purification, filtration, photocatalyst purification and the like are combined, microalgae air purification is achieved, and the air purification effect is improved.

The application relates to a biological cell culture device and a culture method, and relates to the technical field of cell culture. The device comprises a culture box main body with an open upper side. A placing mechanism and a pressing mechanism are arranged in the culture box main body. Before a cylinder is removed, the upper end of the cylinder is first sealed by a sealing plate. Therefore, after the culture dish is removed, external air cannot enter the culture box main body from the corresponding cylinder. When the subsequent culture dish enters, only the gas with the same volume as the cylinder enters the box, and the influence on the gas concentration in the culture box main body is not obvious. The gas concentration environment in the box is protected. Then, the adherent cells are separated from the inner wall to form a suspension liquid through centrifugation. The splashing of the culture liquid is avoided by the sealing plate. Then, the culture liquid is stirred at a low speed by a stirring mechanism. Since the adherent cells are separated from the inner wall in advance and move with the culture liquid, the damage to the cells in the stirring process is reduced, and the uniform distribution of the culture liquid is ensured.

The invention relates to the technical field of fermentation devices, and discloses a special lactobacillus reuteri fermentation device which comprises a fermentation tank, a connecting pipe, an insertion pipe and a sampling pipe, a plug body is slidably arranged on the inner side of the connecting pipe, a bent pipe is inserted into the inner side of the plug body, a retention cylinder is arranged on the inner side of the insertion pipe, and a liquid inlet pipe is inserted into the side wall of the retention cylinder; a first sealing piece is movably arranged at the end of the liquid inlet pipe, the liquid inlet pipe is in intermittent butt joint with the bent pipe, a liquid outlet pipe is inserted into the side wall of the retention cylinder, a second sealing piece is movably arranged at the end of the liquid outlet pipe, and the sampling pipe is in sliding fit with the liquid outlet pipe. The plug body ascends to enable a culture solution in the fermentation tank to enter the retention cylinder through the connecting pipe, the plug body moves downwards to reset, so that the culture solution in the fermentation tank and the culture solution in the retention cylinder are separated independently, the interior of the fermentation tank is not affected and polluted by external operation, and the sampling pipe extracts the culture solution in the retention cylinder to complete sampling. The culture solution in the fermentation tank is prevented from being polluted, so that the fermentation effect is improved.

The application provides a kind of nasal mucosa in vitro culture method and culture fluid, the composition of the culture fluid includes Wnt / β-catenin signal pathway activator 0.1-20 μg / ml, fibroblast growth factor 10-150 ng / ml, TGF-β inhibitor 0.02-3 μg / ml, ROCK inhibitor 0.1-100 μmol / L, P38 MAPK inhibitor 0.1-1 μmol / L, serum-free additive 1X, N-acetyl cysteine 1-10 mmol / L, nicotinamide 1-20 mmol / L, GlutaMax additive 1X, antibiotic, DMEM / F12 culture medium 1X.The application can carry out in vitro culture to nasal mucosa, can keep at least 2 weeks of activity, and have complete epithelial barrier and immune system, can realize ex vivo nasal mucosa virus infection experiment.

The utility model relates to a strain separation tool for field sampling, and belongs to the technical field of strain separation. Comprising a separation tank, a placement mechanism and a springback mechanism, a cabinet door is rotationally connected to one side of the separation tank, the placement mechanism comprises a placement disc, a connecting rod and a limiting disc, the two ends of the connecting rod are fixedly connected with the center positions of the placement disc and the limiting disc correspondingly, and a sliding rod is arranged at the upper end of the placement disc; and a spring which is fixedly connected with the separating tank and abuts against the sliding rod is arranged in the sleeve. By arranging a spring, a sleeve and a springback mechanism which are matched with the placement disc, the separation pipe can vibrate in the carrying and moving process of a worker, fragments with strains can make full contact with a culture solution, the strains can be fully blended into the culture solution, jolt caused by movement of the worker to the device is ingeniously utilized, and the separation efficiency is improved. The device is simple, light and convenient to use during field sampling.

The invention discloses a culture optimization method of brevibacillus laterosporus and application of brevibacillus laterosporus in inhibiting pathogenic bacteria of hawthorn, an LB liquid culture medium is adopted in the culture process, and the culture medium comprises the following components: 10 g / L peptone, 15 g / L sucrose and 10 g / L yeast powder; the initial pH of fermentation is 7-8, the culture temperature is 30-35 DEG C, the rotating speed is 180-200rpm, the culture time is 24-36 hours, and the inoculum size of the brevibacillus laterosporus CB-528 bacterial liquid is 3-5vol%, and finally obtaining the fermented bacterial liquid through fermentation. The zymophyte liquid prepared on the basis of the method can be used for preparing biopesticides for preventing and treating Chinese yam diseases. The fermentation process disclosed by the invention is simple to control, easy to operate and obvious in effect, and the thallus yield and the spore rate can be greatly improved under the fermentation process. The fermentation culture solution disclosed by the invention has the characteristics of high activity, high viable organism content and high spore rate, and can prolong the preservation time of a subsequent preparation and improve the drug effect stability of the preparation.

The invention relates to the technical field of biomedical treatment, and particularly provides a control method of a cell culture workstation and the cell culture workstation. Specifically, the cell culture workstation comprises a box body, a culture device, a first liquid storage component and a heater, the box body is provided with a culture chamber, the culture device and the first liquid storage component are located in the culture chamber, the first liquid storage component is communicated with the culture device, and the control method comprises the steps that the temperature T1 of a culture solution in the first liquid storage component is obtained; acquiring a set temperature Ts of cell culture; and selectively starting a heater to heat the culture solution in the first liquid storage component according to T1 and Ts. A culture solution is heated through a heater, the temperature of the culture solution is increased, the temperature of the culture solution meets the cell culture requirement, cell growth is facilitated, whether the heater needs to be started to heat the culture solution or not is judged by detecting the temperature of the culture solution, and it is better guaranteed that the temperature of the culture solution meets the cell culture requirement; therefore, the growth of cells is facilitated.

The invention provides a bacterium inspection device and a bacterium inspection method. A bacterium inspection device (1) is provided with: one or more containers (2) for storing a bacterium solution (23) containing bacteria (7) and a culture solution; a sensor (22) that is provided to the bottom (21) of each of the one or more containers (2) and that has a detection region (S) for bacteria (7) above the upper surface of the sensor (22); a lid (32) that seals the bacteria (7); and a spacer (26) that supports the cover (32) in a state in which the cover (32) does not come into contact with the upper surface of the sensor (22).

1. A flow control tray for algal culture fluid, comprising: a grounding layer extending along a grounding surface from a first end to a second end; and a plurality of ridges extending in a first direction from the grounding layer, wherein the grounding layer is configured to guide the flow of the algal culture fluid on the grounding surface from the first end to the second end, forming a general flow direction parallel to the grounding layer; each of the plurality of ridges is spaced apart in a direction perpendicular to the grounding surface and comprises a support configured to support an algal culture membrane; and each of the plurality of ridges preferably has a three-dimensional shape configured to divert the culture fluid from the general flow direction.

The invention relates to the field of bacterial culture instruments, in particular to a device for liquid culture of helicobacter pylori. According to the helicobacter pylori culture medium oscillation device, a liquid culture medium can be oscillated more sufficiently and uniformly, so that culture substances are dispersed among helicobacter pylori more uniformly, and strains are in contact with oxygen more sufficiently, so that the growth speed and survival rate of the helicobacter pylori are increased more effectively. The device comprises a supporting limiting frame, a bottom plate frame, a motor and the like. The bottom plate frame is fixedly connected to one side of the lower portion of the supporting limiting frame, and the motor is fixedly connected to the bottom plate frame. According to the invention, the liquid culture medium is subjected to alternate rotary oscillation and left-right reciprocating oscillation, so that the helicobacter pylori in the liquid culture medium is uniformly mixed with the culture solution, the growth speeds of strains above and below the culture medium are kept consistent as far as possible, and the helicobacter pylori can be in full contact with oxygen; therefore, the growth speed and the survival rate of the helicobacter pylori are improved.

The invention provides a microorganism culture system and method.The culture system is used for culturing a to-be-detected object and at least detecting whether microorganisms exist in the to-be-detected object or not, the culture system comprises a rotary culture detection mechanism, and the rotary culture detection mechanism comprises a rotary culture roller and a first driving unit; a plurality of bottle sleeves used for loading culture bottles are arranged on the rotary culture roller, the bottle sleeves are arranged in the circumferential direction of the rotary culture roller, and the first driving unit is used for driving the rotary culture roller to rotate, so that the bottle sleeves rotate in the circumferential direction of the central axis of the rotary culture roller; the axis of the rotary culture roller is inclined, and the included angle between the axis of the bottle sleeve and the axis of the rotary culture roller is smaller than 90 degrees, so that when the bottle sleeve circumferentially rotates along the axis of the rotary culture roller, the bottle sleeve is not in the same plane, but the angle between the bottle sleeve and the horizontal direction is changed, and blood and culture liquid in the culture bottle can be fully mixed. And the culture effect of the culture bottle is ensured.

The application provides a method for producing indoor seed potatoes by using solid culture medium and liquid culture medium, and the method comprises the following steps: in the initial stage, potato stem segments are inoculated by using a solid culture medium containing 3.0% sucrose and 10 g / L agar, and the inoculated potato stem segments are cultured at 24 DEG C under 4000 lux illumination (16 hours / day) for 25 days; 25 ml of liquid culture solution is added; in the later stage, the potato stem segments are transferred into a tuber induction environment, the humidity is maintained through a microporous air permeable film, and double-waveband light signal combination is applied to synergistically promote tuber formation and enlargement; the tuber induction rate and the weight of single potato are significantly improved through accurate regulation of the photoperiod and dynamic switching of the culture medium; the whole process is completed in a closed container, the re-invasion of viruses is effectively prevented, the malformation rate and the moldy rate of the produced potatoes are low, and the method is suitable for indoor production of virus-free seed potatoes.

The invention discloses a circulating type soilless culture device. The device comprises a water inlet pipe; the cultivation pipeline extends in the liquid flowing direction; the negative-pressure air supplementing device comprises a negative-pressure air supplementing section, the water inlet pipe, the negative-pressure air supplementing section and the cultivation pipeline are sequentially communicated in the liquid flowing direction, the circulation sectional area of the negative-pressure air supplementing section is smaller than that of the water inlet pipe, the negative-pressure air supplementing section is provided with an opening, and the opening is communicated with the negative-pressure air supplementing section. The opening is connected with a first air supplementing one-way valve, and the air inlet direction of the first air supplementing one-way valve is from the external environment to the interior of the negative pressure air supplementing section. The oxygen concentration of a culture solution in the cultivation pipeline can be increased.

The utility model belongs to the crossing field of bioengineering and environmental protection, and particularly discloses a spiral stirring type bioreactor for microorganism culture and carbon sequestration. The spiral stirring type bioreactor comprises a shell, a multi-stage stirring system, a precise temperature control system and a gas-liquid distribution system. Through the innovative structural design, the comprehensive efficiency of the bioreactor is remarkably improved; a unique multi-stage stirring system effectively eliminates a mixing dead angle, so that efficient mass transfer and uniform distribution of a culture solution are realized; meanwhile, a temperature control system is combined, so that the requirement of high-precision constant-temperature culture is met, and a stable microorganism growth environment is maintained with low energy consumption; the gas-liquid distribution system obviously improves the gas utilization rate, and is beneficial to improving the carbon sequestration level. Therefore, the problems of low mixing efficiency, poor temperature control level, low CO2 utilization rate and the like of the traditional bioreactor are effectively solved.

The utility model provides a fermentation system. The fermentation system comprises a tank body for storing a microbial agent, a heating component, a temperature sensor, a culture tank, a pumping mechanism and a stirring mechanism, the tank body is provided with a feeding pipe and a discharging pipe, the temperature sensor is located on the side portion of the discharging pipe, the temperature sensor is arranged at the bottom of the tank body, or the temperature sensor is arranged at the bottom of the tank body and extends into the tank body; the heating part is arranged on the side wall of the tank body and extends into the tank body; the culture tank is provided with a microbial agent feeding pipe, a culture solution feeding pipe and a discharging pipe; the pumping mechanism is connected with the feeding pipe and the discharging pipe, so that the microbial agent in the tank body flows circularly, and the temperature of the microbial agent is uniform; the pumping mechanism is connected with the microbial agent feeding pipe; the stirring mechanism is arranged in the culture tank. The fermentation system can effectively ensure that the temperature of the microbial agent is constant in a required range, and the microbial agent with proper temperature and the culture solution are fully mixed to realize fermentation culture, so that the fermentation efficiency is improved.