429 results about "Culture fluid" patented technology

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention discloses a submerged plant culture and root exudates circulating collection device, and relates to the technical field of exudates collection, the submerged plant culture and root exudates circulating collection device comprises a culture box, the bottom of the culture box is fixedly connected with a plurality of connecting rods, the ends, away from the culture box, of the connecting rods are fixedly connected with a collection box, and the side, away from the connecting rods, of the culture box is movably connected with a top cover; a lamp is fixedly connected to the side, close to the incubator, of the top cover, a plurality of culture fixing pipes are movably connected to the interior of the incubator, a secretion collecting assembly is arranged on the incubator, and the secretion collecting assembly comprises a threaded rod and an extrusion rod which are rotationally connected to the interior of the incubator, and a spring and an inclined block which are symmetrically and movably connected to the interior of the incubator; the threaded rod rotates to drive the extrusion rod to rotate, the extrusion rod rotates around the special inclined block in a sliding mode, root exudates can be more effectively released into a culture solution through the exudates collecting assembly, and therefore the exudates collecting efficiency is improved.

The utility model relates to the field of culture frames, and discloses a seedling planting culture frame which comprises a water storage tank, a chassis is fixedly connected to one side of the upper surface of the water storage tank, and a motor is fixedly connected to the center of the lower surface of the water storage tank; according to the device, the motor is connected with the stirring paddle, so that the stirring paddle is driven by the motor to rotate, water in the water storage tank and a culture solution are fully stirred and mixed by the stirring paddle, and then the spray head is communicated with the water storage tank through the hose; a water pump sprays liquid in a water storage tank from the position of a spray head through hoses on the two sides, redundant culture liquid flows out of a water outlet in the bottom of a culture cylinder and flows into a base plate to be stored, then the redundant culture liquid flows into the water storage tank from the position of a through hole, spraying on nursery stocks again is facilitated, and resource waste is reduced; the effects of conveniently spraying the culture solution to the seedlings and collecting and utilizing the redundant culture solution are achieved.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.

The invention belongs to the technical field of mushroom cultivation, and discloses an earthing cultivation mushroom cultivation box which is used as an independent unit for fermentation, mushroom cultivation and fruiting of earthing cultivation mushrooms and comprises an open box body and an aeration layer arranged at the bottom of the box body, and an inner air channel and an inner water channel are embedded in the box body. The inner air channel is provided with an aeration port in the aeration layer in the box body, gas entering the aeration layer from the aeration port enters a culture medium covering the aeration layer from an opening in the surface of the aeration layer, and the inner water channel is provided with an inner nozzle for spraying a culture solution into the box body at the upper part of the culture medium in the box body. The movable platform is used for bearing a plurality of box bodies to form a culture module, and a main air passage and a main water passage are arranged at the lower part of the movable platform and are used for supplying air and water to each opposite box body. The cost is reduced.

The present invention relates to the technical field of Haematococcus pluvialis cultivation, and specifically relates to a disinfection device for a Haematococcus pluvialis cultivation system, which includes an algae storage tank, a disinfection tank, and a number of cultivation tubes. A separation part is connected and arranged between two adjacent upper and lower ones among a number of cultivation tubes on the same side. By starting the double-headed motor to drive the partition to rotate 90 degrees, a number of liquid passing boxes and connecting tubes are blocked, so as to cut off two adjacent cultivation tubes at the same height, and at the same time, the two adjacent upper and lower cultivation tubes are connected to form a loop path. Then, the disinfectant with a large density sinks into the lower cultivation tube to form a layer, and it can be pumped and drained, while the Haematococcus pluvialis culture solution is retained in the cultivation tube for continuous cultivation. Then, just open the liquid outlet pipe to release the disinfectant. Therefore, it is not necessary to lead out and collect all the Haematococcus pluvialis culture solution and then separate the disinfectant. This device saves the time and process of disinfection and separation, and avoids the long-term adverse effects of the disinfectant on the Haematococcus pluvialis culture solution.

The invention relates to a method for electrically activating oocytes based on nanosecond pulses. Single oocytes are sequentially and uniformly arranged between two parallel electrodes of an electrode dish, the electrode dish is characterized in that the distance between the electrodes is 1 mm, the electrodes are placed in a culture dish, and the two ends of the two electrodes are sealed; a culture solution is firstly put between the electrodes which are arranged in parallel, mineral oil is covered to prevent the culture solution from being evaporated, after preheating treatment, oocytes are sequentially and uniformly put between the two electrodes which are arranged in parallel one by one, and then nanosecond pulse electric activation treatment is applied. The electrodes which are arranged in parallel are adopted, the oocytes are sequentially and uniformly placed between the electrodes in a single mode, the situation that the electric pulse effect is reduced due to adhesion and stacking of all the cells is avoided, the oocytes receive uniform and equivalent electric pulse sequences, the action effect and stability of electric pulses on the cells are obviously improved, and the operation efficiency of the oocytes is improved. And the reliability and reproducibility of a test conclusion are improved.

The invention discloses a biological reaction device and a stirring method, and relates to the technical field of bioreactors, the biological reaction device comprises a container, an execution device, a sensor and a control device, the execution device comprises a base and a plurality of stirring paddles arranged on the base, the base is detachably installed on the container, the stirring paddles can be inserted into the container, and the sensor is arranged on the container. Each stirring paddle comprises a rotating shaft and a plurality of paddles, the paddles are inclined to the horizontal plane, the rotating directions of every two adjacent stirring paddles are opposite, liquid in the containing cavity can flow along the rotating axis of the stirring paddles in the rotating process of the stirring paddles, and the mixing effect of the cells and the culture solution is improved. The sensor is inserted into the channel of the rotating shaft and extends out of the stirring paddle, parameters in a culture solution can be detected in real time, and the control device can receive a detection signal of the sensor and control the rotating speed of the stirring paddle based on the detection signal, so that full contact and reaction of cells and the culture solution are guaranteed, and the biological culture effect is improved.

The invention relates to the technical field of agricultural seedling raising, and discloses a seed hydroponic germination incubator for agricultural seedling raising, which comprises an incubator body and a plurality of cultivated seeds placed in the incubator body, and is characterized in that a supporting plate used for bearing the cultivated seeds is arranged in the incubator body, an annular sliding frame is arranged on the outer side wall of the supporting plate, and the annular sliding frame is arranged on the outer side wall of the supporting plate. The annular sliding frame is connected with a floating plate through a connecting rod, the side wall of the incubator body is provided with a liquid supplementing pipe for supplementing and replacing the culture solution into the incubator body, and the liquid level of the culture solution in the incubator body is a liquid level line. The length of the pushing assembly is the same as that of the connecting rod, so that it can be guaranteed that in the lifting and rising process of the pushing assembly, cultivated seeds located above the pushing assembly can be pushed to rise together and are just located at the liquid level line position, and the cultivated seeds are located at the liquid level line position; therefore, the germinated cultivated species are pushed out of the supporting plate to reach the liquid level line, and the environment where the germinated cultivated species are located is changed.

The utility model discloses a double-sleeve continuous flushing negative pressure aspiration liquid culture medium set, which comprises an aerobic culture bottle, an anaerobic culture bottle and a culture bottle placing box, the aerobic culture bottle and the anaerobic culture bottle are arranged in the culture bottle placing box, an air cleaning component and an injection component are arranged in the anaerobic culture bottle, drainage liquid is stored in the injection component, and the drainage liquid is filled in the culture bottle placing box. And the air removal assembly exhausts air in the drainage liquid in the injection assembly. The device has the advantages that culture flasks containing aerobic and anaerobic culture solutions are stored in the culture flask placement boxes, the culture flasks can be opened for use during sampling, the negative pressure connector and the liquid inlet connector are directly connected with the negative pressure tube and the double sleeves, negative pressure sampling of drainage liquid is achieved, operation can be achieved by a single person, medical staff are prevented from making contact with the drainage liquid, and the air removal assembly is used for removing air from the drainage liquid. The air in the drainage liquid in the injection tube is discharged, and then the drainage liquid with the air removed is injected into the anaerobic culture bottle through the injection assembly, so that the air is prevented from entering the anaerobic culture liquid, and the accuracy of a culture result is ensured.

The invention discloses a multi-layer uniform mixing method for microorganism culture, which comprises the following steps: putting: putting microorganisms to be cultured and a culture solution into test tubes, and putting the test tubes into a plurality of culture cavities arranged in a culture box layer by layer in batches; temperature regulation: independently regulating and controlling the temperature in the plurality of culture cavities by a temperature regulation and control mechanism; uniformly mixing: the test tubes revolve and rotate in the incubator, and meanwhile, the test tubes are pushed to oscillate in a small amplitude under the action of an oscillation mechanism, so that the microorganisms and the culture solution are uniformly mixed. The incubator is provided with a plurality of culture cavities, bin doors are hinged to the front sides of the culture cavities, the incubator comprises trays, rotating discs, placement grooves, oscillation blocks and a temperature regulation and control mechanism, the trays are installed in the culture cavities in a sliding mode, the rotating discs are rotatably arranged at the tops of the trays, and the placement grooves rotate while rotating along with revolution of the rotating discs. The invention is used for solving the problem of inaccurate detection result caused by poor mixing effect of the existing mixing device.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The utility model discloses a root exudate collecting device which comprises a culture tube, a partition plate is detachably connected into the culture tube, a positioning hole is formed in the partition plate, and cavities, located on the upper side and the lower side of the partition plate, in the culture tube are filled with glass beads respectively; an extraction assembly is installed on the extraction tank, the input end of the extraction assembly is fixedly communicated with the bottom end of the culture tube, and the output end of the extraction assembly is fixedly communicated with the side wall of the top end of the extraction tank; the cleaning assembly is mounted in the extraction tank; the filter assembly comprises a liquid discharge pipe fixedly connected to the bottom of the extraction tank, one end of the liquid discharge pipe is fixedly communicated with a water inlet of the infusion pump, and a filter is mounted on the liquid discharge pipe; and the output end of the infusion pump is fixedly communicated with the storage box through a conveying pipe. The glass beads are used for replacing soil to cultivate plants, culture liquid is directly discharged through the bottoms of the culture tubes during extraction, plant root systems cannot be affected, and damage to the plant root systems is reduced.

The utility model belongs to the technical field of microbial agent culture, and particularly relates to a microbial agent improved saline-alkali soil culture device which comprises a culture box and a shaking assembly arranged in the culture box, the shaking assembly comprises a rotating disc, a transmission rod, a supporting plate, a stabilizing frame, a lap joint frame and a layered net frame, a transmission groove is formed in the bottom face of the transmission rod, and the supporting plate is arranged in the transmission groove. And the supporting plate is located on the upper side of the transmission rod, a rolling contact rolling wheel is arranged on the bottom face of the supporting plate, and the rolling wheel is rotationally installed on the upper side of the transmission rod. The culture dishes in the culture box can be shaken in different states through the shaking assembly, so that a culture solution is always kept in a dynamic mixing state, the dynamic environment of the saline-alkali soil is simulated, precipitation of saline-alkali components is avoided, the activity and reproduction efficiency of a microbial agent are improved, and the improvement effect of the microbial agent on the saline-alkali soil is enhanced.

The invention relates to the technical field of chrysanthemum breeding methods, in particular to a method for accelerating chrysanthemum breeding by using a liquid culture medium, which comprises the following steps: cutting chrysanthemum stalks into square blocks of 1cm * 1cm in a culture box, flatly laying the stalks upwards on an induction culture solution soaking base, cutting stem tissues with axillary buds into small sections of 2-3cm, and obliquely inserting the small sections into the induction culture solution for culturing, mS + 6-BA 2.0 mg / L +, NAA 0.1 mg / L, alpha-naphthylacetic acid 2-4 mg / L, 6-benzylaminopurine and cane sugar are selected as culture solutions, in the propagation culture of the chrysanthemum, stem seedling sections grow fast, each section generates a plurality of clustered seedlings, the average multiplication coefficient is 4-7 times, the height of the stem seedlings is different, the clustered seedlings are 5-7 cm high when the seedlings are cultured in the nutrient solution for 2 months, and the adventitious bud multiplication rate of the chrysanthemum seedlings is increased. The method has the effect of accelerating chrysanthemum reproduction.

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

The invention discloses a method for increasing the content of ergosterol in tremella aurantialba mycelia by using adenosine, and relates to the technical field of biological ferment.The method comprises the following steps that a sterilized liquid culture medium is inoculated with tremella aurantialba strains, and liquid culture is conducted to obtain a liquid culture solution; centrifuging the liquid culture solution to obtain mycelia; the content of ergosterol in the obtained mycelium is greater than 34.6 mg / L; a liquid fermentation culture medium is composed of 200 g / L of potatoes, 20 g / L of glucose, 3 g / L of monopotassium phosphate, 1.5 g / L of magnesium sulfate and 12.0 mg / L of vitamin B1, water is added to make the volume constant to 1000 mL, and the pH value is natural; adding adenosine into the liquid culture medium until the concentration of adenosine is greater than 1.0 g / L; the classification of the inoculated tremella auramtialba strain is named as Naematia auramtialba, and the classification is named as Naematia auramtialba; the preservation number of the strain is CGMCC (China General Microbiological Culture Collection According to the method, the content of ergosterol in the obtained tremella aurantialba mycelia can be increased to 45.8 mg / L and the biomass to 12.5 g / L under the condition that the biomass of the mycelia of the tremella aurantialba No. 2 ZJJE002 (the preservation number is CGMCC NO.40124) in the tremella aurantialba strain is not influenced, and the content of ergosterol is 1.55 times that of a control group.

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

A plurality of liquid storage bags and waste liquid bags are arranged in a liquid storage area, a liquid separation chip and a plurality of peristaltic pumps and electromagnetic valves are arranged in a liquid separation area, four reaction chips are arranged in a reaction area, each reaction chip can support two parallel experiments, and the different areas are connected through hoses. A culture solution in the liquid storage area is controlled by a pump valve and a liquid separation chip in the liquid separation area, flows into different reaction chips, is subjected to microbial culture and interaction experiments with materials, and finally flows into a waste liquid bag. The reaction chip can be used for collecting electrochemical signals, and real-time observation can be carried out through a microscope. The chip card box provided by the utility model is designed to be totally closed, is high in integration level, can support simultaneous operation of a plurality of reactions, and is safe and reliable.

The utility model discloses a microalgae incubator and belongs to the technical field of incubators. The microalgae culture box comprises a box body, a containing cavity is formed in the box body, a plurality of partition plates are arranged in the box body at intervals in the first direction, and microalgae culture bottles are placed on the partition plates; the light source assembly is arranged on the side wall of the box body; the first pipeline assembly comprises a first main pipe and a plurality of first branch pipes, the plurality of first branch pipes are communicated with the first main pipe, one end of each first branch pipe is inserted into a culture solution of the microalgae culture bottle, and the first branch pipes are used for conveying carbon dioxide into the microalgae culture bottle; and the shaking table module is arranged at the bottom of the partition plate and is used for uniformly shaking the culture solution in the microalgae culture bottle. According to the utility model, the growth speed of the microalgae is increased, and the rapid cultivation of the microalgae is facilitated.

The invention discloses an organoid culture monitoring device which comprises a culture layer and a plurality of sensing modules, the culture layer is provided with a plurality of culture grooves used for culturing organoid, and the culture grooves are filled with conductive culture liquid. Each sensing module is arranged in one culture tank, and the sensing end of the sensing module is submerged in the culture solution and is used for monitoring the growth condition of the organoids in the culture tanks; the sensing module comprises a first insulating substrate and a detection unit, the first insulating substrate is attached to the groove wall of the culture groove in a matched mode so as to surround the organoid located in the culture groove or cells used for culturing the organoid, and the detection unit is arranged on the side, facing the organoid or the cells, of the first insulating substrate; and a groove-shaped sensing interface surrounding the cells or the organoids is formed in the culture groove. According to the technical scheme, the groove-shaped sensing interface is formed, so that non-contact, non-destructive, in-situ and long-time-history synchronous monitoring of the organoids is effectively realized.

The utility model discloses an escherichia coli culture device capable of supplementing materials for multiple times. The Escherichia coli culture device capable of supplementing materials for multiple times comprises a culture dish box and a culture dish cover, an isolating inner ring cylinder is integrally mounted in the culture dish box, an overflow port is formed in the isolating inner ring cylinder, and a first isolating plate and a second isolating plate are integrally mounted on the isolating inner ring cylinder and the inner wall of the culture dish box. According to the Escherichia coli culture device capable of supplementing materials for multiple times provided by the utility model, the isolation inner ring cylinder is arranged in the culture dish box, and the first isolation plate and the second isolation plate are arranged between the culture dish box and the isolation inner ring cylinder, so that a liquid discharge cavity is formed among the isolation inner ring cylinder, the first isolation plate, the culture dish box and the second isolation plate; and the bottom of the liquid discharge cavity is arranged to be an inclined plane, so that the discharged culture liquid can be automatically and continuously collected towards the liquid extraction tank, and the residue of the waste culture liquid can be reduced as much as possible.

The utility model relates to and discloses a microbial fermentation culture container which comprises a fermentation tank body, the fermentation tank body comprises an outer shell inner layer and an inner shell, the inner shell is arranged in the outer shell inner layer, a barrel-shaped temperature adjusting cavity is reserved between the outer shell inner layer and the inner shell, heat conduction liquid is filled in the barrel-shaped temperature adjusting cavity, and a water heater is further installed in the barrel-shaped temperature adjusting cavity. According to the microorganism bacterium fermentation culture container, the water heater can generate heat to heat water in the barrel-shaped temperature adjusting cavity by being communicated with a circuit in the water heater, and then the heated water provides extra heat for microorganism bacterium liquid culture liquid in the barrel-shaped temperature adjusting cavity through the inner shell, so that the needed fermentation temperature is maintained; when the water temperature in the barrel-shaped temperature adjusting cavity is not uniformly distributed, the inner shell can be driven to rotate in the inner layer of the outer shell by connecting a circuit in the motor, and the rotating inner shell is used for simultaneously stirring the microbial bacterium liquid culture solution and the water in the inner ring and the outer ring, so that the microbial bacterium liquid culture solution and the water in the inner ring and the outer ring are uniformly heated.

To provide a culture apparatus in which microalgae do not accumulate on the inner bottom surface of the containment section. [Solution] The culture apparatus 10 comprises a containment section 20 capable of containing culture solution CS and microalgae, a guide section 40 for guiding culture gas G, and a gas supply section 30 for supplying culture gas G to the culture solution CS. The supply pipe section 36 constituting the gas supply section 30 has a main pipe section 362 extending along the depth direction of the containment section 20 and a sub-pipe section 363 extending along a protruding direction intersecting the depth direction. The outlet 38 formed in the sub-pipe section 363 is located below the lower end 43 of the guide section and is formed in a portion of the sub-pipe section 363 that protrudes in the protruding direction from the lower end 43 of the guide section.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

A flow control tray for an algae culture fluid, comprising: a base layer extending from a first end to a second end in a base plane; and a plurality of ridges extending from the substrate layer in a first direction, where the substrate layer is adapted to direct flow of an algae culture solution above the substrate plane from the first end to the second end to form an overall flow direction parallel to the substrate layer, where each of the plurality of ridges includes a support, the support exits the substrate plane in a direction perpendicular to the substrate plane and is adapted to support an algae culture membrane wherein each of the plurality of ridges has a three-dimensional shape preferably adapted to bypass the culture fluid in the overall flow direction.

The invention discloses an in-vitro real-time electromagnetic exposure experiment device based on a conformal antenna. The in-vitro real-time electromagnetic exposure experiment device comprises the following steps: 1) designing the conformal antenna as a radiation source by using a formula; 2) placing the conformal antenna on a light through hole of a microscope objective table; 3) obtaining parameters of the culture solution model by using a meniscus formula, and establishing a culture solution model with a meniscus; 4) placing the circular culture dish filled with the culture solution in the middle of the conformal antenna; and 5) carrying out electromagnetic simulation on the conformal antenna parameters, the circular culture dish and the culture solution through software, carrying out grid division on the culture solution, and finally obtaining SAR distribution in the culture solution. Results show that the method can record the physiological activities of the cells in real time, and meanwhile, the SAR uniformity is also obviously improved.

The present invention relates to the technical field of agricultural disease microorganism detection equipment, and particularly relates to an air spore density capture and analysis device, which includes an outer chassis and an inner chassis arranged inside the outer chassis. A plurality of air intake grilles for absorbing air are provided on both side walls of the outer chassis; a working platform is arranged inside the inner chassis, and a working station turntable is rotatably connected to the working platform. Spore capture units, spore sampling units, spore culture units, and spore microscopic photography units are circumferentially arranged on the edge of the working station turntable. A film covering unit and a culture solution titration unit are arranged between the spore sampling unit and the spore culture unit; the present invention sucks the outside air into the outer casing, so that the spores in the air freely adhere to the glass slide, and monitors the adhesion situation by collecting and culturing the spores on the glass slide. At the same time, the image is sent to an external controller for information analysis, and the analysis result is sent to the monitoring center in the background, so as to realize remote operation control.