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Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin

A technology for large-scale cultivation of Haematococcus pluvialis, which is applied in the field of large-scale cultivation of Haematococcus pluvialis and the transformation of astaxanthin, which can solve problems such as temperature and light contradictions, and achieve fast water exchange and stable water environment , the effect of increasing economic benefits

Active Publication Date: 2007-05-23
LIJIANG CHENGHAI BAOER BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a device and method for large-scale cultivation of Haematococcus pluvialis and transformation of astaxanthin, which can solve the contradiction between the temperature and light required for cultivation and transformation in the cultivation process, and ensure that pluvialls Haematococcus grows rapidly, produces enough biomass, and can ensure that the cultured Haematococcus pluvialls can quickly transform and accumulate astaxanthin

Method used

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  • Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin

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Embodiment 1

[0021]Embodiment one: a kind of device of large-scale culture Haematococcus pluvialis and conversion astaxanthin, it comprises fixed frame (figure not shown) and the photobioreactor system 1 that is arranged on the fixed frame, aeration device 2, culture The liquid infusion device 3 and the cell collection device 4, the photobioreactor system 1 is composed of five unit photobioreactors connected in series, the unit photobioreactor is made of flexible impermeable polyethylene material, and each unit photobioreactor The bioreactors are connected in series through hoses, and the hoses between the unit photobioreactors are provided with an emergency switch 5. The inflatable device 2 is composed of an air pump 21 and an acid liquid container 22. The air pump 21 is connected with the acid liquid through the hose. The container 22 is connected, and the culture fluid infusion device 3 is composed of a first water pump 31 and a culture fluid tank 32. The first water pump 31 is connected...

Embodiment 2

[0022] Embodiment 2: Other structures are the same as Embodiment 1, except that the unit photobioreactor is made of rigid plexiglass.

Embodiment 3

[0023] Embodiment three: Other structures are identical with embodiment one, and difference is that photobioreactor system 1 is made up of ten unit photobioreactors, and unit photobioreactor is made of flexible polyethylene material, and the first unit A culture fluid circulation pipeline (not shown) is arranged between the photobioreactor 11 and the second unit photobioreactor 12, and the culture fluid circulates in the photobioreactor system 1, which can improve the utilization rate of aeration.

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Abstract

The invention discloses a set of apparatus and a method for culturing Haematococcus pluvialis and converting astaxanthin in large scale. The apparatus consists of a photo-bioreactor system set on the frisket, aeration equipment, culture fluid inoculation equipment and still cell harvesting equipment. Floating or sunken pulse equipment is set on the frisket. The apparatus don't need establishment of artificial culture pool and other institution, but uses natural water body to culture Haematococcus pluvialis in large scale. It is low cost, the culture temperature, illumination and water flow rate is stable, culture fluid flows with each other, aeration is continuous, culture conditions are precedent, which make the growth and increment of Haematococcus pluvialis almost at equal pace. The growth rate of Haematococcus pluvialis can reach to 25%, biomass increase is fast, doubles 3-5 days. The photo-bioreactor can be hoisted into required depth of the water, which solves the contradiction of difference of temperature, illumination and nutrition of Haematococcus pluvialis culture and astaxanthin conversion.

Description

technical field [0001] The invention relates to a cultivation device of Haematococcus pluvialis, in particular to a device and method for large-scale cultivation of Haematococcus pluvialls and transformation of astaxanthin. Background technique [0002] Haematococcus pluvialis is a single-celled green alga whose proliferation depends on cell division. The algae can accumulate a large amount of astaxanthin at a specific stage of growth. Astaxanthin has good coloring properties and strong antioxidant properties, and is currently mainly used in aquaculture as a feed colorant and additive for salmon and trout to improve the growth and physiological functions of salmon and trout; at the same time It is also an excellent additive for human food and health products, which can improve immunity and inhibit cancer. Therefore, the application in medicine also has great potential. [0003] It can be seen that Haematococcus pluvialis is an economical microalgae with good development pr...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N1/12C12P23/00
CPCC12M23/06C12M21/02C12M23/56Y02P20/582
Inventor 骆其君严小军周洪马斌尤仲杰
Owner LIJIANG CHENGHAI BAOER BIOLOGICAL DEV
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