308 results about "Astaxanthin" patented technology

The invention provides a saccharomyces cerevisiae engineering strain HYB05 capable of releasing astaxanthin in a controllable self-splitting manner. The saccharomyces cerevisiae engineering strain HYB05 is constructed by taking a basic strain for producing astaxanthin as a starting strain, introducing a coding sequence comprising cell wall lyase Lyc, a coding sequence of a signal peptide mutant F8A and a recombinant nucleic acid construct for expressing a regulatory element, and knocking out an SSD1 gene to relieve a translation inhibition effect. The cell wall lyase Lyc is found and disclosed by the inventor for the first time, and the amino acid sequence is shown as SEQ ID NO: 6; the amino acid sequence of the signal peptide mutant F8A is as shown in SEQ ID NO: 8. According to the engineering strain HYB05, by introducing the recombinant nucleic acid construct, the activation of the catalytic activity of cell wall lyase under specific conditions is realized, so that the cell wall of the saccharomyces cerevisiae is broken, and the astaxanthin synthesized by the saccharomyces cerevisiae is released. Compared with the prior art, the astaxanthin is produced through fermentation of the strain, the extraction steps are simplified, extraction of the astaxanthin in the yeast is achieved to the maximum extent, the yield of the astaxanthin is remarkably increased, and the technical effect is remarkable.

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention discloses microcapsule powder for covering the oil smell of euphausia superba oil and a preparation method of the microcapsule powder, and belongs to the field of euphausia superba oil processing. The microcapsule powder is composed of 50-75% of OSA starch, 10-30% of euphausia superba oil, 3-20% of sodium alginate, 1-10% of succinic acid and 0.5-5% of calcium hydrophosphate, during preparation, the OSA starch is gelatinized and then sheared and homogenized with the euphausia superba oil to obtain a miniemulsion, the succinic acid, the sodium alginate and the calcium hydrophosphate are prepared into a mixed solution, the mixed solution is mixed with the miniemulsion in proportion, and spray drying is performed to obtain a finished product. An OSA starch-sodium alginate double-wall material system is adopted, a compact cross-linked layer is formed through pH response, peculiar smell substances such as trimethylamine can be completely masked, the astaxanthin retention rate is up to 95.14%, the embedding rate reaches 89.48%, krill oil oxidation is effectively inhibited, krill oil is converted into powder, the stability and the sensory property are improved, application of krill oil in the food field is widened, the technology is simple, and the method is suitable for industrial production. The method is suitable for industrial production.

A trace element supplement consisting of lutein, zeaxanthin, astaxanthin, anthocyanins, and proanthocyanidins is designed to improve retinal health in children and adolescents, relieve eye strain, and slow the progression of myopia. The daily dose is 1–20 mg lutein, 0.2–4 mg zeaxanthin, 0.5–8 mg astaxanthin, 10–700 mg taurine, and 0.5–8.5 mg zinc; the nighttime dose is 1–100 mg eyebright extract, 10–300 mg chamomile extract, 1–200 mg anthocyanins, and 1–500 mg proanthocyanidins.

The invention discloses a separation method or an analysis method of astaxanthin geometric isomers and corresponding optical isomers thereof, and relates to the technical field of analysis. According to the method, four geometric isomers of all-trans astaxanthin, 9-cis astaxanthin and 13-cis / 15-cis astaxanthin can be effectively separated, and effective separation and detection of three optical isomers of all-trans astaxanthin, four optical isomers of 9-cis astaxanthin and eight optical isomers of 13-cis / 15-cis astaxanthin are further realized; therefore, effective separation and analysis of optical isomers corresponding to various geometric isomers can be realized. The method is high in specificity, good in separation degree and high in analysis accuracy of geometric isomers and optical isomers, and has important significance in developing research on the accurate correlation between the astaxanthin biological activity and the isomers.

The invention relates to astaxanthin microcapsule powder adopting a freeze spray drying technology and a production process of the astaxanthin microcapsule powder, and discloses the astaxanthin microcapsule powder which realizes astaxanthin activity protection, powder forming and continuous industrial preparation under a low-temperature condition through a synergistic strategy of embedding a composite wall material and regulating emulsion stability by glycerol, and the production process of the astaxanthin microcapsule powder. The astaxanthin microcapsule powder adopting the freeze spray drying technology is characterized by comprising the following components in parts by weight: 1-3 parts of astaxanthin oil, 20-40 parts of a wall material system, 0.2-0.5 part of an emulsifier, 0.1-0.3 part of an antioxidant and 30-50 parts of a water-phase medium, the wall material system is a composite wall material system and comprises gelatin, maltodextrin and hydroxypropyl-beta-cyclodextrin, and the water-phase medium is a water-phase medium. The wall material comprises gelatin, maltodextrin and hydroxypropyl-beta-cyclodextrin, and the gelatin accounts for 40%-60%, the maltodextrin accounts for 30%-50%, and the hydroxypropyl-beta-cyclodextrin accounts for 10%-20%.

The invention discloses an application of astaxanthin in preparation of a regulator for improving the gentisic acid level. On the basis of non-targeted metabonomics analysis, it is found for the first time that in the SAP pathogenesis process, the level of a flora metabolite, namely gentisic acid (GA), in intestinal contents is remarkably reduced, and in-vivo gentisic acid level can be remarkably callback through astaxanthin intervention. Further research proves that exogenous supplement of gentisic acid can effectively simulate the protection effect of astaxanthin, including improvement of pancreatic tissue pathological damage, reduction of serum lipase and amylase activity, and inhibition of systemic inflammatory response (down-regulation of IL-1beta, IL-6 and TNF-alpha and up-regulation of IL-10). The invention not only discloses a new mechanism that astaxanthin plays a therapeutic role through an'intestinal flora-metabolite 'axis, but also determines that gentisic acid is used as a novel drug target for treating SAP, and constructs a new method for screening drugs for treating SAP according to the novel drug target.

The invention provides an anti-aging composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises Inca inchi oil, sandalwood seed oil, Morocco nut oil, olive fruit oil, a rosemary extract, vitamin E, Pinus densiflora leaf oil, valerian oil, astaxanthin and palmitoyl pentapeptide-4. According to the invention, a high-permeability matrix is constructed by utilizing scientific proportioning of a plurality of natural vegetable oils. The Pinus densiflora leaf oil, valerian oil and palmitoyl pentapeptide-4 have a synergistic effect, collagen synthesis is effectively promoted, and neuroinflammatory skin conditions are inhibited, so that aging caused by pressure is effectively resisted. And in combination with a specific anti-oxidation system, the technical problem that highly unsaturated grease is easy to oxidize and deteriorate is solved. The composition disclosed by the invention has excellent effects of relieving, repairing, resisting wrinkles and tightening; the essential oil is stable in property, comfortable in skin feeling and suitable for facial essential oil and other skin care products.

1. Name of the designed product: packaging box (astaxanthin). 2. Use of the designed product: the designed product is used for packaging astaxanthin products. 3. Design points of the designed product: the combination of shape and pattern. 4. Picture or photo that best indicates the design points: front view.

The invention provides an extraction and purification process for producing astaxanthin based on a fermentation method, which specifically comprises the following steps: step 1, preparing materials: selecting shrimp shells, cleaning the shrimp shells, removing impurities doped in the shrimp shells, and then putting the cleaned shrimp shells into a dehydration device for dehydration; step 2, pretreatment; 3, extraction: putting the pretreated shrimp shells into an extraction kettle for extraction; step 4, separation; and step 5, saponification treatment. When the device is used, shrimp shells enter the inner container through the feeding pipe, a cleaning agent enters the water inlet pipe, the cleaning agent enters the inner container through the filtering holes to soak the shrimp shells, and the supporting columns drive the stirring plates to rotate to stir materials during soaking; and in the later period, the supporting column drives the stirring plate to rotate and stir, and meanwhile the materials are conveniently pushed to the position between the connecting cylinder and the discharging cylinder through a scraper on the connecting column.

The invention discloses bacteriostatic gel for gynecology and a preparation method thereof, and relates to the technical field of bacteriostatic gel. The antibacterial gel is prepared from a gel matrix, a plant extracting solution, antibacterial peptide, a humectant, astaxanthin microcapsules and a specific buffer solution. The buffer solution contains sodium citrate, hyaluronic acid and citric acid, and intelligent regulation and control of the drug release behavior of the astaxanthin microcapsule are realized by utilizing the synergistic effect of a hyaluronic acid and citric acid buffer system and preferably adopting hyaluronic acid compounded or modified with different molecular weights. The gel can maintain stable drug release in a normal vagina and pathological fluctuation pH environment, and trigger accelerated release in a high active oxygen inflammation environment, so that the problems that the release behavior is greatly influenced by environmental fluctuation and cannot adapt to different disease stages in the prior art are effectively solved, accurate and personalized drug delivery is realized, and the drug delivery efficiency is improved. The antibacterial agent has the advantages of good antibacterial effect and high environmental adaptability.

The invention discloses a method for preparing high-quality euphausia superba powder by using euphausia superba shelling byproducts, which comprises the following steps: shelling euphausia superba cooked at low temperature, taking the euphausia superba shell byproducts, performing low-temperature vacuum drying until the moisture is below 15%, and crushing and sieving the obtained euphausia superba crisp substances to obtain the high-quality euphausia superba powder. According to the method disclosed by the invention, the euphausia superba unshelling byproducts are turned into wealth from waste, the high-quality euphausia superba meal which is superior to the traditional whole euphausia superba meal in key indexes is successfully prepared through precise process control, and a stable and excellent special raw material basis is provided for the high-end euphausia superba oil industry. According to experimental screening, the krill meal with the high astaxanthin content is obtained at the cooking temperature of 70 DEG C, the cooking time of 25 min and the vacuum drying temperature of 60 DEG C, the astaxanthin content reaches up to 4670.82 mu g / g oil and is far higher than that of krill meal prepared in the prior art, and breakthrough progress is achieved.

This invention provides a portable colorimetric detection system for astaxanthin based on a smartphone, belonging to the field of colorimetric detection technology. Its features include: an optical detection platform for accommodating the Yeast lipolyticis fermentation broth sample and providing a standardized optical detection environment; a smartphone with its camera pointed at the sample area of ​​the optical detection platform for acquiring images of the fermentation broth sample; a color analysis module embedded in or connected to the smartphone for extracting color feature parameters from the acquired images; and a quantitative model module pre-stored with a linear regression model based on the color parameters of the fermentation broth and the astaxanthin concentration, the model being constructed based on the (R+G) / 2 parameter, used to calculate the astaxanthin concentration in the sample based on the color feature parameters. The advantages of this invention are: simplified detection steps, convenient and rapid sample detection, and real-time sample monitoring.

The utility model discloses a mixing device for producing astaxanthin fish oil, and belongs to the field of fish oil production devices. Comprising a mixing tank body, an upper cover is arranged on the mixing tank body, and a feeding port used for feeding astaxanthin powder into the mixing tank body is formed in the upper cover; the fixed ring is fixed in the mixing tank body and is positioned on the upper side of the feeding hole; the rotating ring and the fixed ring are coaxially arranged, and the rotating ring is rotationally arranged on the fixed ring; the fixing frames are circumferentially fixed to the rotating ring, and the fixing frames are located on the lower side of the feeding opening; and the screening frame is arranged on the fixed frame in a sliding manner. The mixing device for astaxanthin fish oil production has the beneficial effects that through the arrangement of the rotating ring and the fixed frame, in the rotating process of the rotating ring, the fixed frame is driven to move, and meanwhile, the moving of the screening frame is matched, so that astaxanthin powder is uniformly screened out into the mixing tank body through the through holes in the screening frame.

The invention relates to the field of antioxidant skin care products, and mainly relates to astaxanthin essence with a natural antioxidant effect and a preparation method of the astaxanthin essence. The invention relates to astaxanthin essence with a natural anti-oxidation effect. The astaxanthin essence is prepared from a water phase, a liquid phase and a liquid phase, wherein the water phase comprises 15-30% of a natural plant extracting solution, 3-8% of a humectant, 0.3-1.5% of a thickening agent and a preservative; an oil phase: 5%-14% of vegetable fat, 1%-4% of lecithin, 0.5%-2% of a rosemary extract and 0.3%-1.5% of a green tea extract; 0.8% to 4% of an astaxanthin liposome; arabic gum; a pH adjusting agent; water; the natural plant extracting solution comprises aloe juice and cucumber juice. By adopting natural raw materials and an innovative process, the stability and bioavailability of astaxanthin are improved, and the oxidation resistance of the essence is enhanced; meanwhile, it is ensured that the product is natural and mild, irritation to skin is reduced, and the requirements of consumers for high-quality natural skin care products are met.

The invention discloses a double-response controlled-release hydrogel for promoting wound healing of astaxanthin and an amino small molecule drug as well as a preparation method and application of the hydrogel, and belongs to the field of biomedical materials. The material is composed of a drug-loaded nano-micelle and a self-healing hydrogel network. Hydrophilic modification of astaxanthin and dynamic coupling of the astaxanthin and an amino small molecule drug are achieved through an astaxanthin-polyethylene glycol-benzaldehyde intermediate, and the drug-loaded nano-micelle has the pH response drug release capacity; the hydrogel network is formed by crosslinking phenylboronic acid modified carboxymethyl chitosan and dopamine through dynamic phenylboronic acid ester bonds, and has ROS responsiveness and self-healing characteristics; the multifunctional intelligent wound dressing can intelligently respond to high ROS and acidic pH microenvironment of chronic wounds, controllably release astaxanthin and amino-containing small molecule drugs, synergistically remove ROS, improve cell oxidative stress, inhibit inflammation, promote blood vessel and tissue regeneration and accelerate chronic wound healing, is excellent in self-healing performance and biocompatibility, and is an ideal multifunctional intelligent wound dressing.

The invention relates to the technical field of energy storage batteries, in particular to an electrolyte, a lithium-rich manganese battery and a preparation method of the lithium-rich manganese battery. The electrolyte comprises a solvent, a lithium salt, an additive and astaxanthin, the additive comprises a lithium salt additive and / or a solvent additive. According to the technical scheme, astaxanthin is introduced into the electrolyte and used for the lithium-rich manganese battery, active oxygen is efficiently captured through conjugated double bonds and hydroxyl structures in molecules of the electrolyte, electrolyte decomposition and positive electrode structure damage are inhibited, the first coulombic efficiency of the battery is improved through addition of astaxanthin, and it is indicated that astaxanthin participates in formation of a stable interfacial film, so that the lithium-rich manganese battery is obtained. The first-circle active lithium loss is reduced. Meanwhile, along with the introduction of astaxanthin, the cycle retention rate is also improved.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The invention discloses a Pickering emulsion stabilized by full-component egg white protein-EGCG (epigallocatechin gallate) as well as a preparation method and application of the Pickering emulsion, and belongs to the field of functional carrier emulsion preparation. The full-component egg white protein-EGCG composite stabilizer is formed by compounding a cooked egg white protein microparticle solution and an EGCG solution through pH regulation and control, the full-component egg white protein-EGCG composite stabilizer can be used for well stabilizing a Pickering emulsion and loading bioactive substances; the prepared Pickering emulsion with the stable astaxanthin-loaded full-component egg white protein-EGCG compound has relatively strong self-supporting property, relatively high encapsulation efficiency, excellent rheological property, moisture distribution property and biologically active substance slow release property, can be used as a 3D printing material, and has good 3D printing stability and accuracy.

To provide compositions for improving vision, in particular, corrected vision, uncorrected vision and / or practical vision.SOLUTION: A composition for improving one or more types of visions selected from uncorrected vision, corrected vision and practical vision contains a component (A) or contains the component (A) and a component (B). The component (A) is at least one selected from astaxanthin and esters of the astaxanthin. The component (B) is vitamin E.SELECTED DRAWING: Figure 1

This application relates to a method for improving the utilization of soybean protein in salmonid fish. This disclosure provides a method for modifying salmonid fish to grow on a diet containing soybean protein. The method includes administering a fish feed composition to the fish for an effective period after hatching. The fish feed composition contains soybean protein and an effective amount of an antioxidant. The antioxidant may be astaxanthin.

The invention belongs to the technical field of separation and extraction of natural products, and particularly relates to a process for extracting astaxanthin by using a deep eutectic solvent, which comprises the following steps: extracting an astaxanthin raw material and the deep eutectic solvent in an ultrasonic-pulsed electric field synergistic device according to a mass-volume ratio of 1: 15-1: 25 g / mL, and then purifying by using a modified macroporous resin column to obtain the astaxanthin. And finally, carrying out film concentration and spray drying to obtain an astaxanthin product. The deep eutectic solvent is prepared from the following components in parts by mass: 15-25 parts of a hydrogen bond acceptor, 35-55 parts of a hydrogen bond donor and 1-3 parts of a functionalized nano material, the functionalized nano material is silanization modified mesoporous silica. According to the method disclosed by the invention, efficient extraction, high product stability, low solvent residue and good environmental compatibility of astaxanthin are synchronously realized through a synergistic effect of a plurality of technical characteristics of functionalized nano material, ultrasonic-pulsed electric field synergistic extraction, modified macroporous resin purification, protective agent application and the like.

The invention discloses a preparation rich in polygonatum sibiricum polypeptide and application of the preparation in diabetes mellitus. The composition is prepared from rhizoma polygonati polypeptide, semeglutide, astaxanthin, an active agent and a ferroptosis mixture. Pharmacodynamic experiments show that in a diabetic mouse model, fasting blood glucose can be remarkably reduced, the serum insulin level can be increased, renal function indexes can be improved, and expression of NLRP3 inflammation pathway related proteins (NLRP3, Caspase-1 and IL-1beta) in pancreatic tissue can be remarkably inhibited. By integrating multiple mechanisms of blood glucose reduction, oxidation resistance, inflammation resistance, ferroptosis resistance and the like, multi-target collaborative intervention is realized, the pharmaceutical composition is remarkably superior to a single component in the aspects of blood glucose reduction, pancreas islet function protection and complication alleviation, and a new clinical strategy is provided for treatment of diabetes mellitus, especially type 1 diabetes mellitus.