645 results about "Astaxanthin" patented technology

The invention discloses application of astaxanthin as a feed additive in improving the disease resistance of macrobrachium rosenbergii, and belongs to the field of feed additives. According to the method, astaxanthin is supplemented in the feed, the survival rate of the juvenile macrobrachium rosenbergii after being infected with DIV1 is increased, meanwhile, the abundance of pathogenic bacteria in intestinal tracts after the macrobrachium rosenbergii is infected with DIV1 is reduced, then the purpose of preventing disease outbreak of the macrobrachium rosenbergii caused by DIV1 can be achieved, and the method is a sustainable and effective method for preventing and treating the macrobrachium rosenbergii DIV1 disease.

The invention discloses a recombinant neurospora crassicola strain for producing astaxanthin as well as a construction method and application of the recombinant neurospora crassicola strain. The recombinant neurospora crassa contains key enzymes beta-carotene ketolase and beta-carotene hydroxylase in a heterologous astaxanthin synthetic pathway, and through pathway expression of a targeted subcellular compartment, crosstalk among metabolites is effectively prevented, metabolic flux of a biosynthetic pathway is coordinated, and the activity of the neurospora crassa is improved. The effective contact between the heterologous protein and the substrate in the astaxanthin synthetic route is promoted, the sporulation capability of the heterologous protein is improved, the astaxanthin production and storage capability of the neurospora crassiforme is effectively improved, and the method has relatively high popularization and application values.

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention provides a culture method for improving immunity of fishes. The culture method comprises the following steps: S1, selection of culture points; S2, specification and layout of net cages; S3, selection and throwing of fish fries; according to the fry disinfection method disclosed by the invention, the fry are soaked and disinfected by using a compound disinfectant prepared from povidone iodine, polysorbate, citric acid and vitamin C in a specific proportion, so that pathogenic microorganisms on the surfaces and gill parts of fish bodies are effectively killed. According to the breeding management, the immunity of the fish body is comprehensively enhanced by feeding the fish with an immune conditioner composed of campesterol palmitate, immunomodulatory peptide, beta-glucan and astaxanthin in a specific proportion. According to the bottom mud management, the content of harmful substances is reduced by putting a bottom mud modifier composed of sodium molybdate, nitrifying bacteria, photosynthetic bacteria, tetrandrine and sodium alginate in a specific proportion, growth of pathogenic bacteria is inhibited, nutrient substance circulation is promoted, the water quality environment is optimized, and healthy growth of fry is promoted.

The invention discloses a schizochytrium limacinum strain for producing astaxanthin and DHA (docosahexaenoic acid) and application of the schizochytrium limacinum strain, the schizochytrium limacinum strain is schizochytrium limacinum AST32-IEIZW-12, the classification name of the schizochytrium limacinum strain is schizochytrium limacinum sp.AST32-IEIZW-12, the strain schizochytrium limacinum sp.AST32-IEIZW-12 is preserved in China General Microbiological Culture Collection Center, the preservation number is CGMCC No.41544, the preservation time is October 11, 2024, the preservation number is CGMCC No.41544, the preservation number is CGMCC No.41544, and the preservation number is CGMCC the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. According to the method disclosed by the invention, the joint production of the astaxanthin and the DHA in the thraustochytrium limacinum is realized, the DHA yield can reach 2.8 g / L, and the astaxanthin yield can reach 19.58 mg / L (2.04 mg / g).

The invention relates to the technical field of preparation of astaxanthin sustained-release microspheres, and discloses chitosan-based astaxanthin sustained-release microspheres and a preparation method thereof.The method comprises the steps that chitosan powder is dissolved in an acetic acid solution, the pH value is adjusted, and a chitosan solution is prepared; dissolving astaxanthin in ethanol, and then adding the chitosan solution; adding a cross-linking solution into the emulsified solution, and performing cross-linking under a magnetic stirring condition to form astaxanthin sustained-release microspheres; adding the astaxanthin sustained-release microspheres into a strengthening solution for strengthening treatment; carrying out coating treatment on the astaxanthin sustained release microspheres subjected to the strengthening treatment; carrying out centrifugal treatment on the solution subjected to coating treatment, and collecting astaxanthin sustained-release microspheres subjected to centrifugal separation; and washing the collected astaxanthin sustained-release microspheres with PBS (Phosphate Buffer Solution). According to the invention, chitosan is used as a carrier to prepare the astaxanthin sustained-release microspheres, so that the astaxanthin can be continuously and stably released in intestinal tracts, the antioxidant and anti-inflammatory activity of the astaxanthin can be fully exerted, and further, the astaxanthin sustained-release microspheres have an effective treatment effect on osteoarthritis.

The invention discloses a health-care composition, and belongs to the field of health-care foods. Every 1000 mL of the composition contains 1.7 to 1.9 g of lutein ester, 0.52 to 0.61 g of zeaxanthin, 0.35 to 0.47 g of astaxanthin, 30 to 60 g of a chrysanthemum extract, 40 to 70 g of celery powder, 30 to 60 g of Lithocarpus litseifolius powder, 110 to 130 g of a sweetening agent and the balance of purified water. According to the composition provided by the invention, all the raw materials are compounded for use, so that a remarkable asthenopia relieving effect is achieved, and eye health is favorably promoted.

The invention relates to the technical field of functional food, in particular to an eye-protecting and brain-strengthening composition as well as a preparation method and application thereof. The aronia, the astaxanthin, the DHA, the medlar, the hovenia dulcis thunb and the saffron crocus extract are compounded, various functional raw materials have a synergistic effect, the visual problem is improved through multiple channels, the cognitive ability is synchronously improved, and the astaxanthin eye drops have the effects of preventing retina oxidative damage, promoting eye blood circulation, relieving visual fatigue, protecting nerve cells and improving the cognitive state. Through reasonable proportioning, the composition for protecting eyes and strengthening brain has better effects of relieving visual discomfort, improving vision and improving brain health.

The invention provides a saccharomyces cerevisiae engineering strain HYB05 capable of releasing astaxanthin in a controllable self-splitting manner. The saccharomyces cerevisiae engineering strain HYB05 is constructed by taking a basic strain for producing astaxanthin as a starting strain, introducing a coding sequence comprising cell wall lyase Lyc, a coding sequence of a signal peptide mutant F8A and a recombinant nucleic acid construct for expressing a regulatory element, and knocking out an SSD1 gene to relieve a translation inhibition effect. The cell wall lyase Lyc is found and disclosed by the inventor for the first time, and the amino acid sequence is shown as SEQ ID NO: 6; the amino acid sequence of the signal peptide mutant F8A is as shown in SEQ ID NO: 8. According to the engineering strain HYB05, by introducing the recombinant nucleic acid construct, the activation of the catalytic activity of cell wall lyase under specific conditions is realized, so that the cell wall of the saccharomyces cerevisiae is broken, and the astaxanthin synthesized by the saccharomyces cerevisiae is released. Compared with the prior art, the astaxanthin is produced through fermentation of the strain, the extraction steps are simplified, extraction of the astaxanthin in the yeast is achieved to the maximum extent, the yield of the astaxanthin is remarkably increased, and the technical effect is remarkable.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention discloses new application of Pseudomonas sp. F13, namely application of the Pseudomonas sp. F13 in splitting haematococcus pluvialis to extract astaxanthin, the Pseudomonas sp. F13 and the haematococcus pluvialis are jointly fermented and cultured, the Pseudomonas sp. F13 can split the haematococcus pluvialis, so that the astaxanthin in the haematococcus pluvialis is fully released, and the extraction rate reaches 76%. The method is simple and easy to operate, low in cost and suitable for industrial production. Solvents and wall breaking equipment are not needed in extraction, so that the negative influence on the environment is reduced, and the method is suitable for industrial production.

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The invention belongs to the technical field of medicines, and discloses an astaxanthin and fish oil compound for assisting in reducing blood sugar as well as a preparation method and application of the astaxanthin and fish oil compound. The astaxanthin and fish oil compound for assisting in reducing blood sugar comprises the following raw materials: astaxanthin oil and fish oil. The invention also discloses an application of the astaxanthin and fish oil compound for assisting in reducing blood sugar in preparing a medicine for reducing blood sugar. The astaxanthin and fish oil compound prepared by the invention is simple in component, high in antioxidant activity and remarkable in blood sugar reducing effect, and has a wide application prospect in the aspect of treating diabetes mellitus.

The invention discloses a spirulina extracellular vesicle-loaded astaxanthin hydrogel as well as a preparation method and application thereof, and astaxanthin is loaded into spirulina extracellular vesicles to form spirulina extracellular vesicle-loaded astaxanthin nanoparticles. And then, the nano-particles are contained in self-assembled hydrogel composed of aldehyde hyaluronic acid and carboxymethyl chitosan, and the spirulina extracellular vesicle astaxanthin-loaded hydrogel is prepared and obtained. The preparation method comprises the following steps: firstly, preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel, and preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel. The hydrogel prepared by the invention can realize synergistic and complementary effects of spirulina extracellular vesicles and astaxanthin, realizes a protective effect on radiodermatitis by relieving oxidative stress, recovering mitochondrial functions and reducing inflammatory response, and shows long-term biological safety.

The invention relates to the technical field of astaxanthin oil, in particular to a preparation method of astaxanthin oil, which comprises the following specific steps: mixing haematococcus powder with double-particle-size glass beads, adding coconut oil, and oscillating; then cellulase is added for a hydrolysis reaction, enzyme is inactivated after hydrolysis, and a wall-broken mixture is obtained; carrying out primary extraction and secondary extraction on the wall-broken mixture, combining the two extracting solutions, and filtering; performing supercritical CO2 extraction on the filtrate to obtain primary astaxanthin oil; mixing the primary astaxanthin oil with a 1-butyl-3-methylimidazole hydroxide ethanol solution, and introducing nitrogen to carry out a reaction; cooling and filtering after the reaction is finished, and finally desolventizing under reduced pressure to obtain the astaxanthin oil. In the design, the 1-butyl-3-methylimidazole hydroxide can efficiently hydrolyze lipid impurities in the astaxanthin oil to generate water-soluble fatty acid salt and glycerol, so that the separation efficiency is improved, and the separation effect is further optimized.

The invention discloses composite gel with ROS and pH dual responsiveness and a preparation method and application thereof.The preparation method comprises the steps that gold nanoparticles are prepared, gold nanostars GNS grow, a surfactant and biguanide drugs are added to obtain drug-loaded gold nanostars GM, a metal organic framework is introduced to obtain drug-loaded nanoparticles GMZ of a core-shell structure, and the composite gel is prepared. And reacting with a TSPBA solution, an astaxanthin solution and a polyvinyl alcohol PVA solution for crosslinking to obtain the ROS and pH dual responsive composite gel. According to the invention, the gold nanostars are used as a photosensitizer to construct the multifunctional composite gel, and the metal organic framework structure degrades under a slightly acidic condition of a psoriasis epithelium microenvironment to release released zinc ions, so that the gel is endowed with various treatment effects, excellent photo-thermal treatment performance and controlled release performance of the zinc ions and drugs. The gel has ROS responsiveness, can remove excessive ROS in a psoriasis microenvironment, and realizes the treatment of oxidation resistance, inflammation resistance and keratinocyte proliferation inhibition of psoriasis by combining with a nano drug delivery system.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The invention discloses a genetically engineered bacterium and a method for synthesizing astaxanthin by using the genetically engineered bacterium. According to the genetically engineered bacterium, a CrtE gene, a CarRP gene and a CarB gene are expressed in an original strain. The genetically engineered bacterium disclosed by the invention can produce beta-carotene and astaxanthin at high yield, and the activation culture of the genetically engineered bacterium does not need to add a defective culture medium or antibiotics, so that the genetically engineered bacterium is beneficial to industrial research of large-scale fermentation of astaxanthin.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention discloses a culture medium for increasing the yield of paracoccus astaxanthin and application of the culture medium. The culture medium comprises the following components according to concentration: 4-20g / L of yeast extract; 8-20 g / L of beef extract; 4 to 20 g / L of bacteriological peptone; 10 to 30 g / L of glucose; the concentration of KH2PO4 is 0.1 to 0.2 g / L; the concentration of Na2HPO4. 12H2O is 0.1 to 0.5 g / L; 0.1 to 1 g / L of MgSO4. 7H2O; 0.1 to 1 g / L of FeSO4. 7H2O; the concentration of CaCl2. 2H2O is 0.02 to 0.1 g / L; the pH is 7.2 + / -0.2. The yeast extract, the beef extract and the bacteriological peptone are selected and mixed to serve as a nitrogen source, accumulation of paracoccus biomass and astaxanthin can be promoted synergistically, and meanwhile the problem of precipitation after the culture medium is sterilized is solved.

The invention belongs to the technical field of biological materials, and relates to modified hyaluronic acid with antibacterial and whitening effects and application of the modified hyaluronic acid in a medical beauty filler. According to the preparation method, an ultraviolet irradiation reduction technology is adopted, a hyaluronic acid aqueous solution is used as a polymer dispersion platform, a silver nano colloidal particle dispersion system is constructed, the silver nano colloidal particle dispersion system is converted into HA-Ag powder after being dried, and the HA-Ag powder and carboxymethyl chitosan are mutually cooperated, so that the product is endowed with excellent antibacterial performance. A microcapsule technology is introduced, astaxanthin is selected as a capsule core component, and by virtue of protection of sodium hyaluronate, the stable existence time of astaxanthin is prolonged, and the oxidation resistance of the product is greatly improved. The vitamin C and the arbutin are blended into the microcapsule wall material, and then the microcapsule wall material is mixed with the nicotine amide for use, so that the vitamin C can reduce oxidized astaxanthin while the whitening effect is enhanced, the antioxidant activity of the oxidized astaxanthin is recovered, and the antioxidant effect is further amplified.

The invention provides a fresh tilapia mossambica block preservative and a preparation method thereof, the fresh tilapia mossambica block preservative comprises the following raw materials: natural plant extract, modified biological polysaccharide, organic acid, natural antibacterial peptide, a humectant, deionized water and the like, and propolis extract and astaxanthin can be selected to enhance the effect; wherein the natural plant extract is corncob, zingiber mioga and tartary buckwheat bran extract; the modified biological polysaccharide is obtained by modifying chitosan quaternary ammonium salt, laminarin, cell wall polysaccharide and carboxylic acid. The fresh-keeping agent is prepared through ultrasonic mixing, multilayer electrostatic deposition and other technologies, through the synergistic effect of all the raw materials, a multilayer coating structure is formed through electrostatic deposition, microbial growth can be inhibited, gas exchange can be controlled, water loss can be reduced, the fresh-keeping period of tilapia mossambica blocks can be effectively prolonged, and the quality of the tilapia mossambica blocks can be kept.

The invention relates to the technical field of nano composite materials, in particular to a nano composite containing fat-soluble functional factors as well as a preparation method and application of the nano composite. Debranched starch is subjected to etherification modification, so that the hydrophilicity of the starch is increased, and negative charges are given to the starch; according to the nano-composite material, the nano-composite material is used as a nano-material, then the nano-composite material and alcohol-soluble protein (Zein) are used for composite embedding of curcumin, astaxanthin and other fat-soluble functional factors based on electrostatic, hydrophobic and hydrogen bond interaction, a uniform and regular nano-composite is formed, and the composite is relatively high in pH, ionic strength and thermal stability, small in particle size and relatively high in embedding rate and loading capacity for curcumin, astaxanthin and other fat-soluble functional factors. In addition, a continuous preparation process is adopted, so that the intermediate step of freeze drying and redissolving is avoided, and the process flow is greatly simplified.

The invention belongs to the technical field of biological medicine, and particularly relates to application of astaxanthin vesicles in preventing and relieving colon cancer, and the astaxanthin vesicles have at least one of the following applications: oxidation resistance; anti-inflammation; the antibacterial effect is achieved; the phagocytic ability of macrophages is promoted; the intestinal health is improved; tumor cell proliferation is resisted; preventing and / or treating tumors; the preparation method of the embedding body comprises the following steps: S1, mixing an iRGD peptide ligand, a hyaluronic acid ligand and a solution containing vesicles to obtain a carrier; s2, enabling the carrier to be in contact with astaxanthin, so as to obtain the embedding body. According to the application, the astaxanthin is embedded into the targeting carrier, so that the astaxanthin is effectively protected from being influenced by an in-vivo environment, and meanwhile, the astaxanthin is accurately targeted to target parts such as macrophages in a tumor microenvironment, so that the concentration of a medicine at a diseased region is improved, the side effect on normal tissues is reduced, and the curative effect of the medicine is improved. The astaxanthin can safely and efficiently reach a target site and exert multiple functions of oxidation, anti-inflammation, antibiosis and the like.

The present invention relates to the field of extraction devices, and specifically to an astaxanthin extraction device. An extraction tank is provided with a central shaft driven by a power device and rotating around its own axis. A sleeve is vertically slidably installed on the central shaft, a fixed sleeve is fixedly installed on the sleeve, and a sliding sleeve is also vertically slidably installed on the sleeve located above the fixed sleeve. A stirring blade is fixedly installed on both the fixed sleeve and the sliding sleeve. A movable column is also radially slidably installed on the fixed sleeve along the extraction tank, a scraper matching the inner wall of the extraction tank is fixedly installed on one end of the movable column, and a support rod is hinged between the sliding sleeve and the movable column. While the fixed sleeve and the sliding sleeve rotate to stir the material, the cantilever drives the sleeve, the fixed sleeve and the sliding sleeve to move back and forth up and down along the central shaft through the hollow tube, thereby driving the stirring blade to evenly stir the material at different depths in the extraction tank.

The invention belongs to the technical field of microalgae culture, and particularly provides a haematococcus pluvialis culture medium and a preparation method thereof.The haematococcus pluvialis culture medium is prepared from 1-3 mg / L of sodium acetate, 0.01-1 mg / L of cobalt nitrate hexahydrate, 0.01-1.6 mg / L of copper sulfate, 0.1-1.2 mg / L of zinc sulfate heptahydrate, 1-3 mg / L of manganese chloride tetrahydrate, 1-10 mg / L of boric acid, 1-30 mg / L of sodium carbonate, 1100-1500 mg / L of sodium nitrate, 0.01-6 mg / L of EDTA, 20-50 mg / L of calcium chloride, 70-100 mg / L of magnesium sulfate heptahydrate, 40-100 mg / L of monopotassium phosphate trihydrate and 0.02 g of inorganic phosphorus; the method comprises the following specific steps: S1, culturing haematococcus pluvialis to a stable phase by a conventional method, inoculating haematococcus pluvialis species into a haematococcus pluvialis culture medium, and culturing; s2, shaking table; s3, culturing for 45 hours; s4, inoculating the seed solution into a haematococcus pluvialis culture medium, and continuously culturing in the shaking table for 6-15 days; the culture medium disclosed by the invention has a good promotion effect on the growth of haematococcus pluvialis and the accumulation of astaxanthin.

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.