515 results about "Astaxanthin" patented technology

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention relates to the technical field of preparation of astaxanthin sustained-release microspheres, and discloses chitosan-based astaxanthin sustained-release microspheres and a preparation method thereof.The method comprises the steps that chitosan powder is dissolved in an acetic acid solution, the pH value is adjusted, and a chitosan solution is prepared; dissolving astaxanthin in ethanol, and then adding the chitosan solution; adding a cross-linking solution into the emulsified solution, and performing cross-linking under a magnetic stirring condition to form astaxanthin sustained-release microspheres; adding the astaxanthin sustained-release microspheres into a strengthening solution for strengthening treatment; carrying out coating treatment on the astaxanthin sustained release microspheres subjected to the strengthening treatment; carrying out centrifugal treatment on the solution subjected to coating treatment, and collecting astaxanthin sustained-release microspheres subjected to centrifugal separation; and washing the collected astaxanthin sustained-release microspheres with PBS (Phosphate Buffer Solution). According to the invention, chitosan is used as a carrier to prepare the astaxanthin sustained-release microspheres, so that the astaxanthin can be continuously and stably released in intestinal tracts, the antioxidant and anti-inflammatory activity of the astaxanthin can be fully exerted, and further, the astaxanthin sustained-release microspheres have an effective treatment effect on osteoarthritis.

The invention provides a saccharomyces cerevisiae engineering strain HYB05 capable of releasing astaxanthin in a controllable self-splitting manner. The saccharomyces cerevisiae engineering strain HYB05 is constructed by taking a basic strain for producing astaxanthin as a starting strain, introducing a coding sequence comprising cell wall lyase Lyc, a coding sequence of a signal peptide mutant F8A and a recombinant nucleic acid construct for expressing a regulatory element, and knocking out an SSD1 gene to relieve a translation inhibition effect. The cell wall lyase Lyc is found and disclosed by the inventor for the first time, and the amino acid sequence is shown as SEQ ID NO: 6; the amino acid sequence of the signal peptide mutant F8A is as shown in SEQ ID NO: 8. According to the engineering strain HYB05, by introducing the recombinant nucleic acid construct, the activation of the catalytic activity of cell wall lyase under specific conditions is realized, so that the cell wall of the saccharomyces cerevisiae is broken, and the astaxanthin synthesized by the saccharomyces cerevisiae is released. Compared with the prior art, the astaxanthin is produced through fermentation of the strain, the extraction steps are simplified, extraction of the astaxanthin in the yeast is achieved to the maximum extent, the yield of the astaxanthin is remarkably increased, and the technical effect is remarkable.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The invention discloses a spirulina extracellular vesicle-loaded astaxanthin hydrogel as well as a preparation method and application thereof, and astaxanthin is loaded into spirulina extracellular vesicles to form spirulina extracellular vesicle-loaded astaxanthin nanoparticles. And then, the nano-particles are contained in self-assembled hydrogel composed of aldehyde hyaluronic acid and carboxymethyl chitosan, and the spirulina extracellular vesicle astaxanthin-loaded hydrogel is prepared and obtained. The preparation method comprises the following steps: firstly, preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel, and preparing the spirulina extracellular vesicle astaxanthin-loaded hydrogel. The hydrogel prepared by the invention can realize synergistic and complementary effects of spirulina extracellular vesicles and astaxanthin, realizes a protective effect on radiodermatitis by relieving oxidative stress, recovering mitochondrial functions and reducing inflammatory response, and shows long-term biological safety.

The invention relates to the technical field of astaxanthin oil, in particular to a preparation method of astaxanthin oil, which comprises the following specific steps: mixing haematococcus powder with double-particle-size glass beads, adding coconut oil, and oscillating; then cellulase is added for a hydrolysis reaction, enzyme is inactivated after hydrolysis, and a wall-broken mixture is obtained; carrying out primary extraction and secondary extraction on the wall-broken mixture, combining the two extracting solutions, and filtering; performing supercritical CO2 extraction on the filtrate to obtain primary astaxanthin oil; mixing the primary astaxanthin oil with a 1-butyl-3-methylimidazole hydroxide ethanol solution, and introducing nitrogen to carry out a reaction; cooling and filtering after the reaction is finished, and finally desolventizing under reduced pressure to obtain the astaxanthin oil. In the design, the 1-butyl-3-methylimidazole hydroxide can efficiently hydrolyze lipid impurities in the astaxanthin oil to generate water-soluble fatty acid salt and glycerol, so that the separation efficiency is improved, and the separation effect is further optimized.

The invention discloses composite gel with ROS and pH dual responsiveness and a preparation method and application thereof.The preparation method comprises the steps that gold nanoparticles are prepared, gold nanostars GNS grow, a surfactant and biguanide drugs are added to obtain drug-loaded gold nanostars GM, a metal organic framework is introduced to obtain drug-loaded nanoparticles GMZ of a core-shell structure, and the composite gel is prepared. And reacting with a TSPBA solution, an astaxanthin solution and a polyvinyl alcohol PVA solution for crosslinking to obtain the ROS and pH dual responsive composite gel. According to the invention, the gold nanostars are used as a photosensitizer to construct the multifunctional composite gel, and the metal organic framework structure degrades under a slightly acidic condition of a psoriasis epithelium microenvironment to release released zinc ions, so that the gel is endowed with various treatment effects, excellent photo-thermal treatment performance and controlled release performance of the zinc ions and drugs. The gel has ROS responsiveness, can remove excessive ROS in a psoriasis microenvironment, and realizes the treatment of oxidation resistance, inflammation resistance and keratinocyte proliferation inhibition of psoriasis by combining with a nano drug delivery system.

The invention discloses equipment and a process for efficiently and continuously extracting astaxanthin by a low-frequency microwave-assisted eutectic solvent method, and belongs to the technical field of bioactive substance extraction. The method for extracting astaxanthin by the low-frequency microwave-assisted eutectic solvent method comprises the following steps: performing low-frequency microwave pretreatment on a eutectic solvent to obtain a high-temperature pretreated eutectic solvent; cooling the pretreated eutectic solvent, then mixing the pretreated eutectic solvent with crustacean byproducts, performing eutectic solvent extraction treatment, performing ultrasonic-assisted extraction in the process, and then performing solid-liquid separation to obtain filtrate; mixing the filtrate with an extraction solvent, and extracting to obtain an extraction solvent phase containing astaxanthin and a eutectic solvent phase; and removing the extraction solvent in the extraction solvent phase containing the astaxanthin to obtain an astaxanthin product.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention relates to the technical field of nano composite materials, in particular to a nano composite containing fat-soluble functional factors as well as a preparation method and application of the nano composite. Debranched starch is subjected to etherification modification, so that the hydrophilicity of the starch is increased, and negative charges are given to the starch; according to the nano-composite material, the nano-composite material is used as a nano-material, then the nano-composite material and alcohol-soluble protein (Zein) are used for composite embedding of curcumin, astaxanthin and other fat-soluble functional factors based on electrostatic, hydrophobic and hydrogen bond interaction, a uniform and regular nano-composite is formed, and the composite is relatively high in pH, ionic strength and thermal stability, small in particle size and relatively high in embedding rate and loading capacity for curcumin, astaxanthin and other fat-soluble functional factors. In addition, a continuous preparation process is adopted, so that the intermediate step of freeze drying and redissolving is avoided, and the process flow is greatly simplified.

The invention belongs to the technical field of biological medicine, and particularly relates to application of astaxanthin vesicles in preventing and relieving colon cancer, and the astaxanthin vesicles have at least one of the following applications: oxidation resistance; anti-inflammation; the antibacterial effect is achieved; the phagocytic ability of macrophages is promoted; the intestinal health is improved; tumor cell proliferation is resisted; preventing and / or treating tumors; the preparation method of the embedding body comprises the following steps: S1, mixing an iRGD peptide ligand, a hyaluronic acid ligand and a solution containing vesicles to obtain a carrier; s2, enabling the carrier to be in contact with astaxanthin, so as to obtain the embedding body. According to the application, the astaxanthin is embedded into the targeting carrier, so that the astaxanthin is effectively protected from being influenced by an in-vivo environment, and meanwhile, the astaxanthin is accurately targeted to target parts such as macrophages in a tumor microenvironment, so that the concentration of a medicine at a diseased region is improved, the side effect on normal tissues is reduced, and the curative effect of the medicine is improved. The astaxanthin can safely and efficiently reach a target site and exert multiple functions of oxidation, anti-inflammation, antibiosis and the like.

The present invention relates to the field of extraction devices, and specifically to an astaxanthin extraction device. An extraction tank is provided with a central shaft driven by a power device and rotating around its own axis. A sleeve is vertically slidably installed on the central shaft, a fixed sleeve is fixedly installed on the sleeve, and a sliding sleeve is also vertically slidably installed on the sleeve located above the fixed sleeve. A stirring blade is fixedly installed on both the fixed sleeve and the sliding sleeve. A movable column is also radially slidably installed on the fixed sleeve along the extraction tank, a scraper matching the inner wall of the extraction tank is fixedly installed on one end of the movable column, and a support rod is hinged between the sliding sleeve and the movable column. While the fixed sleeve and the sliding sleeve rotate to stir the material, the cantilever drives the sleeve, the fixed sleeve and the sliding sleeve to move back and forth up and down along the central shaft through the hollow tube, thereby driving the stirring blade to evenly stir the material at different depths in the extraction tank.

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention discloses microcapsule powder for covering the oil smell of euphausia superba oil and a preparation method of the microcapsule powder, and belongs to the field of euphausia superba oil processing. The microcapsule powder is composed of 50-75% of OSA starch, 10-30% of euphausia superba oil, 3-20% of sodium alginate, 1-10% of succinic acid and 0.5-5% of calcium hydrophosphate, during preparation, the OSA starch is gelatinized and then sheared and homogenized with the euphausia superba oil to obtain a miniemulsion, the succinic acid, the sodium alginate and the calcium hydrophosphate are prepared into a mixed solution, the mixed solution is mixed with the miniemulsion in proportion, and spray drying is performed to obtain a finished product. An OSA starch-sodium alginate double-wall material system is adopted, a compact cross-linked layer is formed through pH response, peculiar smell substances such as trimethylamine can be completely masked, the astaxanthin retention rate is up to 95.14%, the embedding rate reaches 89.48%, krill oil oxidation is effectively inhibited, krill oil is converted into powder, the stability and the sensory property are improved, application of krill oil in the food field is widened, the technology is simple, and the method is suitable for industrial production. The method is suitable for industrial production.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The invention provides a crayfish astaxanthin proliposome and a preparation method thereof, and belongs to the technical field of preparation of astaxanthin nano-liposomes. The preparation method of the astaxanthin proliposome provided by the invention comprises the following steps: (1) taking crayfish shells, fully freeze-drying the crayfish shells, grinding and sieving the crayfish shells to obtain crayfish shell powder; (2) taking the shrimp shell powder, adding absolute ethyl alcohol, extracting astaxanthin, and collecting an astaxanthin solution; (3) adding phospholipid into the astaxanthin solution to obtain an astaxanthin-phospholipid solution; and (4) adding sugar alcohol particles into the astaxanthin-phospholipid solution, and carrying out rotary evaporation to remove the absolute ethyl alcohol so as to obtain the granular astaxanthin precursor liposome. By adopting the method disclosed by the invention, the extraction of the crayfish shell astaxanthin and the linkage preparation of the astaxanthin precursor liposome can be realized, and the stable astaxanthin liposome can be quickly and efficiently prepared.

A trace element supplement consisting of lutein, zeaxanthin, astaxanthin, anthocyanins, and proanthocyanidins is designed to improve retinal health in children and adolescents, relieve eye strain, and slow the progression of myopia. The daily dose is 1–20 mg lutein, 0.2–4 mg zeaxanthin, 0.5–8 mg astaxanthin, 10–700 mg taurine, and 0.5–8.5 mg zinc; the nighttime dose is 1–100 mg eyebright extract, 10–300 mg chamomile extract, 1–200 mg anthocyanins, and 1–500 mg proanthocyanidins.

The invention discloses a separation method or an analysis method of astaxanthin geometric isomers and corresponding optical isomers thereof, and relates to the technical field of analysis. According to the method, four geometric isomers of all-trans astaxanthin, 9-cis astaxanthin and 13-cis / 15-cis astaxanthin can be effectively separated, and effective separation and detection of three optical isomers of all-trans astaxanthin, four optical isomers of 9-cis astaxanthin and eight optical isomers of 13-cis / 15-cis astaxanthin are further realized; therefore, effective separation and analysis of optical isomers corresponding to various geometric isomers can be realized. The method is high in specificity, good in separation degree and high in analysis accuracy of geometric isomers and optical isomers, and has important significance in developing research on the accurate correlation between the astaxanthin biological activity and the isomers.

The invention relates to the technical field of krill oil, in particular to a preparation method of krill oil with high phospholipid content. Raw materials adopted by the preparation method at least comprise krill, n-butyl alcohol, beta-cyclodextrin, propylene glycol and water. In the preparation method of the krill oil with high phospholipid content, krill, n-butyl alcohol, beta-cyclodextrin, propylene glycol and water are used as raw materials, and low-temperature multi-time layering operation is performed, so that the phospholipid content in the krill oil is effectively increased, the use amount of an organic solvent is remarkably reduced, the residue of the organic solvent is effectively reduced, and the safety and nutritional value of the product are increased; in addition, in the preparation process, damage to other bioactive components such as Omega-3 fatty acid and astaxanthin is small, and the nutrition and health-care effects of the krill oil are reserved; in addition, according to the method, the process is simplified, the complex treatment step of removing an organic solvent is reduced, the production efficiency is improved, and the production cost is reduced, so that the economic benefit of krill oil production is improved.

The invention discloses a restriction enzymolysis pea protein isolate-chitosan Pickering emulsion which is composed of an oil phase and a water phase, and the water phase is composed of HPP, CS and water; the HPPI is obtained by carrying out enzymolysis on PPI through trypsin. According to the application of the restricted enzymolysis pea protein isolate-chitosan Pickering emulsion in preparation of a medicine embedding preparation, the medicine can be selected from astaxanthin. The invention further discloses the AST embedded Pickering emulsion and the Pickering emulsion yoghourt. According to the method disclosed by the invention, the PPI is subjected to enzymolysis by utilizing an enzymolysis technology to obtain the HPPI with more excellent emulsifying performance, the enzymolysis time is extremely short (2 minutes), the modification cost is favorably reduced in actual production, and the method is easy to realize in industrialization. The research of the invention provides theoretical support for developing a novel delivery system and widening the application range, and lays a foundation for the application of Pickering emulsion in future food.

The invention discloses a method for preparing fermented soybean meal through synergistic fermentation of saccharomyces cerevisiae FNFHSc18 and bacillus subtilis FNFHBS08 and application of the fermented soybean meal, combined fermentation of the saccharomyces cerevisiae FNFHSc18 and the bacillus subtilis FNFHBS08 is utilized, the method has the advantages of being short in fermentation period, high in efficiency and low in cost, the prepared fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is increased. The contents of crude protein and soluble protein are obviously improved, anti-nutritional factors such as antigen protein, oligosaccharide and urease are greatly reduced, and the nutritional value of the soybean meal is comprehensively improved; the invention also provides an application of the fermented soybean meal feed in breeding, and the fermented soybean meal is used for replacing 30% of fish meal protein, so that the final weight, the weight gain rate and the specific growth rate of the largemouth bass can be obviously improved, and the growth performance of bred animals is enhanced.

The invention discloses astaxanthin nano-emulsified instant beverage powder and a preparation method thereof, and relates to the field of astaxanthin, and the astaxanthin nano-emulsified instant beverage powder comprises the following components by mass: 0.1-0.5 part of astaxanthin oil; 10 to 15 parts of a water soluble carrier, wherein the water soluble carrier is selected from hydroxypropyl-beta-cyclodextrin; 1.5-3 parts of an emulsifier; 0.05 to 0.1 part of an antioxidant; 0.01 to 0.03 part of a sensitive pigment; and 20-30 parts of a protective carrier. Astaxanthin molecules are anchored in a water-soluble carrier through nano-emulsification high-pressure homogenization in combination with hydrophobic cavity embedding of hydroxypropyl-beta-cyclodextrin, a dense barrier is formed through physical wrapping in a spray drying stage by utilizing a protective carrier, and meanwhile, an emulsifier is directionally arranged on an oil-water interface and cooperates with an antioxidant to remove free radicals, so that the water-soluble astaxanthin is prepared. The molecular structure of the astaxanthin is stabilized, the apparent solubility of the astaxanthin is increased to 50 mg / L or above, the cold water dissolution time is shortened to be within 20 s, the defect that the dispersity of the astaxanthin in a water system is poor is effectively overcome, and the application of the astaxanthin in the water-soluble beverage is provided.

The invention belongs to the field of biological medicines or health care products, and particularly discloses a culture method and application of an anti-aging stem cell exosome. According to the method, low-oxygen (5%-8% O) pre-culture is combined with rotary three-dimensional dynamic culture, so that the yield of the exosome is remarkably increased to 2.1 * 10 < 11 > parts / mL; tGF-beta1, IFN-gamma and NMN are added in stages to synergistically activate an immunomodulatory pathway, so that the content of TGF-beta1 reaches 8.5 ng / [mu] g. The exosome and active components (vitamin C ethyl ether, Q10 and astaxanthin) are jointly embedded in liposome (HSPC: cholesterol = 7: 3), the gastric juice stability reaches 92.3% after oral administration, and the intestinal absorption rate is increased to 80%. Experiments show that the composition can improve the Treg ratio of the spleen of an old mouse by 35%, reduce the IL-6 level by 48% and improve the skin barrier function (TEWL is reduced by 40%). Long-term toxicity experiments prove that the compound is safe (ALT has no abnormality). The invention has remarkable technical progress and clinical application potential in the anti-aging field.

The invention provides a natural Chinese herbal medicine hydrogel composite microneedle and a preparation method and application thereof.The preparation method comprises the steps that S1, natural food-borne nutrients and glycyrrhizic acid are mixed in a solvent, and a glycyrrhizic acid / natural food-borne nutrient solution is obtained; the natural food-borne nutrient is one or more of astaxanthin, baicalein, berberine and curcumin; s2, dropwise adding a metal salt solution into the glycyrrhizic acid / natural food-borne nutrient solution, and performing ultrasonic treatment and stirring to obtain glycyrrhizic acid / food-borne active substance gel; s3, mixing the polyanionic polymer solution with the glycyrrhizic acid / food-borne active substance gel to obtain a mixed solution; and S4, adding the mixed solution into a microneedle mold, carrying out vacuum centrifugation, and drying to obtain the microneedle patch. Good biocompatibility of the natural Chinese herbal medicine extract glycyrrhizic acid is used as a carrier to increase the in-vivo utilization rate of natural food-borne nutrients, so that a better treatment effect is achieved.

The invention relates to astaxanthin microcapsule powder adopting a freeze spray drying technology and a production process of the astaxanthin microcapsule powder, and discloses the astaxanthin microcapsule powder which realizes astaxanthin activity protection, powder forming and continuous industrial preparation under a low-temperature condition through a synergistic strategy of embedding a composite wall material and regulating emulsion stability by glycerol, and the production process of the astaxanthin microcapsule powder. The astaxanthin microcapsule powder adopting the freeze spray drying technology is characterized by comprising the following components in parts by weight: 1-3 parts of astaxanthin oil, 20-40 parts of a wall material system, 0.2-0.5 part of an emulsifier, 0.1-0.3 part of an antioxidant and 30-50 parts of a water-phase medium, the wall material system is a composite wall material system and comprises gelatin, maltodextrin and hydroxypropyl-beta-cyclodextrin, and the water-phase medium is a water-phase medium. The wall material comprises gelatin, maltodextrin and hydroxypropyl-beta-cyclodextrin, and the gelatin accounts for 40%-60%, the maltodextrin accounts for 30%-50%, and the hydroxypropyl-beta-cyclodextrin accounts for 10%-20%.

The invention discloses an astaxanthin soft capsule and a preparation process thereof, and belongs to the technical field of functional food, the soft capsule comprises a capsule core and a capsule wall; the capsule core is prepared from the following components in parts by mass: 90 to 95 parts of astaxanthin oil, 60 to 64 parts of linseed oil, 8 to 12 parts of gamma-aminobutyric acid and 3 to 5 parts of beewax; the capsule wall comprises the following components in parts by mass: 36-40 parts of gelatin, 20-24 parts of glycerol, 5-7 parts of black pepper extract and 36-40 parts of purified water. The soft capsule obtained by processing the capsule core and capsule wall materials has the advantages of high stability and good disintegration effect.