34 results about "Schizochytrium" patented technology

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The present disclosure provides a Schizochytrium sp. Strain and its use thereof.

This invention discloses a highly absorbable, non-allergenic DHA brain-boosting oral liquid specifically designed for students of all ages, belonging to the field of functional nutritional beverage technology for teenagers. This invention uses high-purity Schizochytrium algae oil DHA and perilla seed active molecules as the core brain-boosting matrix, innovatively configuring a grade-specific ingredient system. The formula is customized for the physical condition and brain function needs of students at different grade levels: kindergarten, primary school, junior high school, high school, and university. Through a small-molecule cell-wall breaking extraction process, the nutrients are directly absorbed into the bloodstream and quickly converted into "brain gold" in the brain. This formula strictly avoids eight major food allergens, ensuring safety for all ages. It also specifically addresses sub-health issues related to learning across all grade levels, including early childhood brain development and emotional well-being, exam preparation efficiency and fatigue reduction for students preparing for higher education, and cognitive development and mental sustainability for university students. The product comes in standardized packaging of 50ml / vial and 15 vials / box, with a customized sweet taste tailored to different grade levels, making it highly acceptable to students. It has no side effects and is suitable for long-term healthy consumption by all school students, effectively improving brain efficiency and learning status across all grade levels, with broad market application prospects.

The present invention relates to a process for preparing a biomass of microorganisms of the genus Schizochytrium or Aurantiochytrium comprising the steps of: a) inoculating, in a bioreactor, a culture medium comprising a brine, preferably a 6% dry extract brine, and nitrogen, preferably at a concentration of between 5 and 15 g / L of culture medium, with an inoculum of microorganisms of the genus Schizochytrium or Aurantiochytrium, said brine being a co-product of the demineralization treatment of whey, b) culturing the microorganisms in said culture medium under conditions, in particular of temperature, pH, agitation and / or oxygen supply, appropriate for the growth of said microorganisms, preferably under non-axenic conditions, and c) collecting the biomass obtained at the end of step b).

This invention belongs to the field of microbial fermentation technology, specifically a process for producing EPA using Schizochytrium fungi as raw material. It employs a dual-gene modified strain with MAT gene overexpression and CLF gene precise knockout, which is domesticated under high-sugar oxidative stress to obtain a highly stable strain. Through three-stage temperature-controlled, stepwise dissolved oxygen fermentation, and the combined addition of para-aminobenzoic acid and ascorbic acid, EPA synthesis is enhanced while oxidative loss is reduced. Highly efficient EPA enrichment is achieved through low-temperature ultrasonic cell disruption of wet cells, urea inclusion, two-stage molecular distillation, and tandem purification using immobilized composite adsorption decolorization. This invention significantly improves EPA yield and proportion, reduces oxidative loss, and enhances product purity and stability through the synergistic effect of gene modification, metabolic regulation, and purification processes. The process is highly controllable, suitable for industrial production, and yields EPA products with high purity and good stability, which can be directly used in the food, health product, and pharmaceutical fields.

The application belongs to the technical field of microbial fermentation, and discloses a method for improving the production of palm oleic acid in Schizochytrium sp. by fermentation regulation and application. In the fermentation process of Schizochytrium sp., one or more combined regulation strategies of controlling the fermentation temperature, optimizing the carbon-nitrogen ratio of the fermentation medium and adjusting the pH value of the fermentation liquid are adopted to change the metabolic flow direction, so as to improve the accumulation of palm oleic acid C16:1 in oil. Compared with genetic engineering modification, the method does not need complex genetic operation and has high safety. Through verification of a 5L fermentation tank, the proportion of palm oleic acid in oil can be significantly increased from less than 1% in conventional fermentation to 16.12%, and the oil yield is stable, so that the method has good industrial application potential.

The application provides a preparation method of a schizochytrium sp. protoplast. The preparation method of the schizochytrium sp. protoplast comprises the following steps: fermenting and culturing schizochytrium to obtain a fermentation liquor; a first fermentation stage: the temperature is 26-28 DEG C, and the culturing time is 36-40 h; a second fermentation stage: the temperature is 30-32 DEG C; and no sugar is additionally supplemented in the process of the fermentation culture. A large amount of protoplasts can be obtained by controlling the culturing conditions in the fermentation culture stage.

This invention belongs to the technical field of laying hen feed additives, specifically relating to a selenium-enriched, high-DHA, low-cholesterol functional egg feed additive and its preparation method. The additive is prepared by compounding selenized ginseng polysaccharide, Schizochytrium algae powder, rosemary extract, natural vitamin E, EGCG green tea extract, phytosterol micron powder, sodium butyrate, β-glucan, phytase, modified attapulgite clay, and maltodextrin in specific weight parts, followed by low-temperature premixing activation, antioxidant encapsulation treatment, low-temperature compound granulation, sieving, and packaging processes. This invention, through the synergistic effect of its components, enables eggs to reach stable functional indicators within 14 days of feeding. The yolk selenium content reaches 32.1~38.2 μg / 100g, DHA content reaches 210.5~261.7 mg / 100g, and cholesterol reduction rate reaches 16.7%~22.3%. Simultaneously, eggshell strength increases by 12.1%~18.5%, egg production rate remains at 95.1%~96.8%, and egg breakage rate decreases by 59.0%~72.0%. The product exhibits good stability, requires low dosage, and is easy to use, making it perfectly suited for large-scale egg-laying hen farming scenarios and possessing outstanding economic and health value.

The feed composition containing the microalgae-derived biomass of the present application does not cause any factors that reduce the quality of the feed, and can be usefully used in fish feed compositions using microalgae that are high in protein, fat, and minerals.

This invention relates to the field of aquaculture technology, and in particular to a biological attractant based on Schizochytrium fermentation broth and its preparation method; the biological attractant based on Schizochytrium fermentation broth is obtained by processing the fermentation broth after oil extraction from Schizochytrium; the fermentation broth after oil extraction from Schizochytrium is the residual fermentation broth after supercritical CO2 oil extraction or enzymatic hydrolysis oil extraction from Schizochytrium; the preparation method includes: pretreatment of fermentation broth, sterilization and stabilization treatment, concentration and regulation, and filling and storage; the three core components in the fermentation broth work synergistically, umami amino acids quickly trigger the taste perception of aquatic animals, algal polysaccharides slowly release odor molecules to prolong the feeding attraction time, and DHA enhances natural tropism, significantly improving the feeding induction rate of Litopenaeus vannamei, and maintaining a stable feeding attraction effect even in rainy and low-temperature environments; relying on the natural viscosity and structural characteristics of algal polysaccharides, there is no need to add stabilizers such as xanthan gum.

The application provides a method for fermenting astaxanthin. The method comprises fermenting astaxanthin by using a Schizochytrium sp. with a preservation number of CCTCC M 2021564 as a fermentation strain. The strain is a mutant strain with high astaxanthin content, which is obtained by using a Schizochytrium sp. screened and separated from seawater as a starting strain and by mutagenesis screening, and the astaxanthin obtained by the strain contains a large amount of DHA astaxanthin ester, and has high economic value and application value.

The invention provides a method for regulating microbial metabolism in the fermentation process of liquor yeast by using marine active peptides, which comprises the following steps: adding marine active peptides in the fermentation process of yeast in the production process of liquor, improving the growth and metabolism state of yeast fermentation microorganisms, optimizing the microbial community structure, promoting the formation of flavor substances and precursors thereof, and improving the yield of liquor yeast. The quality of the yeast and the contribution capability of the yeast to the white spirit flavor are improved; the marine active peptide is chlorella pyrenoidosa peptide, euphausia superba peptide, fish albumin peptide or schizochytrium limacinum peptide. Marine active peptides are introduced into a liquor yeast fermentation system, and different molecular weight intervals, amino acid compositions and peptide sequence structural characteristics of the marine active peptides are utilized, so that differential regulation and control of the yeast fermentation process are realized. Different types of marine active peptides can regulate the growth state and metabolic activity of microorganisms in the fermentation process, and influence the dynamic succession of microbial community structures in a fermentation system and the enrichment behavior of key functional bacteria.

The application discloses a screening marker recovery technology based on a Cre-LoxP combined Flp-Frt recombination system and application. The Cre-Loxp recombination system comprises a Cre recombinase and a LoxP site, and the LoxP nucleic acid combination comprises LoxP71 and LoxP66 sequences which can be specifically recognized and recombined by the Cre recombinase; similarly, the Flp-Frt recombination system comprises a Flp recombinase and a Frt site, and the Frt site comprises a Frt32 sequence which can be specifically recognized and recombined by the Cre recombinase. Due to the lack of screening markers and inducible promoters in the Schizochytrium, the site-specific recombinase cannot be recovered after being integrated into the genome, and therefore, the application aims to combine the two specific recombinase systems, so that the screening markers and the site-specific recombinase can be recovered unlimited times, and thus, the multi-gene editing of the Schizochytrium can be realized.

The invention discloses a fatty acid metabolism regulation gene mining method based on transcriptome data driving, a FindGene platform developed by the invention can efficiently integrate and standardize large-scale RNA-Seq data, systematic mining function related genes are analyzed through a weighted gene co-expression network, and the gene mining efficiency is improved. The limitation of dependence on small-scale experiments and manual screening in the past is broken through, and the efficiency and accuracy of key regulation gene screening are greatly improved. According to the schizosaccharomyces cerevisiae engineering strain QH-7 constructed by carrying out site-directed mutagenesis, overexpression and combined transformation on a key gene obtained by screening, the yield of fatty acid reaches 2.12 g / L and is increased by about 118.6% compared with that of a wild strain, and the biosynthesis efficiency of the fatty acid is greatly improved. According to the modified strain, the total fatty acid content is increased, meanwhile, the proportion of unsaturated fatty acids (C16: 1, C18: 1 and C18: 2) is remarkably increased, and the requirements for high-quality lipid raw materials in the fields of downstream biofuels, nutritional supplements and the like are met.

The invention discloses schizochytrium limacinum polypeptide with a lipid metabolism regulating effect as well as a separation and purification method and application. The separation and purification method comprises the following steps: S1, preparing a schizochytrium limacinum protein suspension; s2, adjusting the schizochytrium limacinum protein suspension obtained in the step S1 to be neutral, adding neutral protease for hydrolysis, cooling after boiling, and hydrolyzing to obtain schizochytrium limacinum protein hydrolysate; s3, the schizochytrium limacinum protein enzymatic hydrolysate obtained in the step S2 is filtered through a membrane, polypeptide with the molecular weight being smaller than 3 kDa and the amino acid sequence being SEQ ID NO.1 is obtained, and the schizochytrium limacinum polypeptide with the lipid metabolism adjusting effect is obtained. According to the invention, accumulation of triglyceride (TG) and cholesterol (TC) in cells is effectively reduced, lipotoxicity stress is relieved, and efficient and low-toxicity candidate active molecules are provided for development of drugs for resisting fatty liver, reducing blood fat or treating metabolic syndrome.

The application discloses a ylang-ylang flower composite repair factor, a preparation method and application, relates to the field of daily necessities, and contains the following components in mass fractions: ylang-ylang flower extract 1-10 parts, hydrolyzed keratin 0.1-5 parts, gynostemma pentaphyllum extract 0.5-8 parts, urea 0.1-3 parts, trehalose 0.5-6 parts, zinc PCA 0.1-3 parts and schizochytrium fermentum filtrate 10-50 parts; wherein the ylang-ylang flower extract is extractum obtained by alcohol extraction and ethyl acetate extraction of ylang-ylang flowers, and the total terpene content is not less than 15% in terms of drimane D; the molecular weight of the hydrolyzed keratin is 500-3000 Da; the ylang-ylang flower composite repair factor, the preparation method and the application construct a ylang-ylang flower extract plus zinc PCA bidirectional oil control channel, and inhibit excessive secretion of sebum from both lipid synthesis in sebaceous gland cells and hormone signals.

The invention discloses deep moisturizing and hydrating gel as well as a preparation method and application of the deep moisturizing and hydrating gel. The composition comprises the following components: bifidus yeast fermentation product filtrate, human-like collagen, hydrolyzed fish collagen peptide, alpha-arbutin, crithmum maritimum callus culture filtrate, eryngium maritimum callus culture filtrate, desert rose callus culture filtrate, peony fermentation filtrate, hydrolyzed placenta (sheep) extract, leukocyte extract and soybean fermentation metabolite. The skin care product is prepared from a brown algae extract, 1, 3-butanediol, sodium hyaluronate, D-panthenol, an aloe extracting solution, allantoin, carbomer, xanthan gum, cyclopentasiloxane (and) cyclohexasiloxane, polyacrylic acid (ester) copolymer sodium (and) lecithin, a hamamelis mollis distillate, a jania rubens extract, arginine, sweet orange essential oil and the balance of water. The anti-wrinkle gel has the advantages that the anti-wrinkle gel is mild and non-irritant, multiple effects of relieving, moisturizing, tightening and anti-wrinkle are synergistic, the gel is not easy to flow, the dosage is convenient to control, no sticky feeling exists, and the experience feeling is good.

The invention discloses a preparation method of an artificial feed for breeding hermetia illucens larvae as well as a product and application of the artificial feed, schizochytrium limacinum algae residues are added into a basic feed raw material, and the artificial feed for breeding the hermetia illucens larvae is obtained through fermentation of bacillus subtilis. Bacillus with lipase activity is added to perform enzymolysis on schizochytrium limacinum residues, so that the problem that artificial feed is thick due to addition of the schizochytrium limacinum residues is solved, and easy-to-absorb algae mud components rich in nutrients such as micromolecular fatty acid and the like are obtained; the feed additive is added into an artificial feed for black soldier fly larvae as a nutrition enhancer so as to achieve the purpose of improving the growth and breeding performance of larvae. The raw materials are derived from schizochytrium limacinum production waste residues, the cost is low, waste utilization is achieved, the related technology facilitates rapid and healthy breeding of larvae in the industrial hermetia illucens breeding process, and high-quality larvae can be provided for spawning of rear-end adults.

The application discloses a kind of schizochytrium high DHA low DPA oil fermentation control method and application, through the time axis joint control strategy of "resource release-pathway bias-state reinforcement": in early and middle fermentation, small molecule of natural source is added in batches, and FAS competition path is moderately inhibited;In key time window, the programmed ventilation and stirring cascade control of "normal oxygen supply-low oxygen supply induction-recovery oxygen supply" is implemented to maintain target DO interval;And 28-34 ℃ temperature ladder is used to up-regulate and can be linked with feeding to strengthen metabolic state switching and fatty acid spectrum formation.After the above-mentioned joint regulation, DHA 67.5%, DPA 4.5% and oil yield 84.3 g / L can be stably obtained in 5 L fermenter;Scaling up to 500 L fermenter, DHA 68.3%, DPA 4.8% and oil yield 89.7 g / L can still be obtained.The method is safe and simple, and the parameters can be engineered to replicate, easy to scale up and migrate, can significantly reduce the burden of DHA / DPA separation and purification and improve the product specification stability, with good industrial application prospect.

The application provides a low-DPA-content thraustochytriales microbial engineering strain and a construction method and application thereof. The thraustochytriales microorganism is specifically a schizochytrium, aureococcus, urkella, thraustochytrium or hondaea. The thraustochytriales microbial engineering strain is obtained by knocking out one allele of a B subunit (OrfB) in a PUFA synthetase, and is a low-DPA-content engineering strain. The fermentation of the engineering strain for producing DHA-containing oil can significantly reduce the DPA content in the oil without affecting the DHA yield; the technical effect is realized by using a gene knockout method, and no exogenous gene is introduced, thereby reducing the possible safety problems in the food field, overcoming the problems in the prior art, and achieving a remarkable technical effect. In addition, the engineering strain improves the upper limit of the DHA content that can be achieved by schizochytrium modification by reducing the DPA content in the total fat, thereby laying an important foundation for subsequent research and development.

The invention provides a preparation method of a microalgae Pickering emulsion, and relates to the technical field of Pickering emulsion preparation.The preparation method comprises the steps that prepared schizochytrium limacinum particles and spirulina particles are mixed with an oil phase and a water phase according to a preset proportion, and an intermediate solution is obtained; and homogenizing and mixing the intermediate solution for a preset time to prepare the microalgae Pickering emulsion, wherein the schizochytrium limacinum particles and the spirulina particles form a uniform and tightly connected network structure in the microalgae Pickering emulsion, so that the oil phase and the water phase form a stable three-dimensional structure system. The preparation method provided by the invention can effectively ensure the long-term storage stability of the Pickering emulsion.

The invention discloses a preparation method of a microalgae carrier and application of the microalgae carrier in immobilized enzyme. According to the method, chlorella, nannochloropsis and schizochytrium are taken as objects, and microalgae residues are modified to be prepared into a microalgae carrier by utilizing pretreatment of a deep eutectic solvent in which the ratio of n (ChCl) to n (glycerol) is 1: 2 in combination with an ultrasonic-assisted technology, and the microalgae carrier is used for immobilizing lipase. According to the method, the utilization rate of microalgae can be increased, resource waste and environmental pollution are reduced, a systematic technical path is provided for full-component high-value utilization of microalgae grease and residues, and application and expansion of green enzyme engineering in the fields of food processing and biological manufacturing are expected to be promoted.

This invention belongs to the fields of biomedicine, marine microbiology, and microbial fermentation, and discloses a high-EPA-producing Schizochytrium fungus and its applications. The Schizochytrium fungus KDW-306 provided by this invention has the accession number CGMCC No. 42323. This Schizochytrium fungus KDW-306 exhibits high biomass, high oil yield, high EPA titer, and high DHA titer, excellent EPA and DHA synthesis capabilities, and excellent production stability and genetic stability through generations, thus well meeting the needs of large-scale industrial production of EPA and DHA.

This invention provides a method for rapidly cultivating P5 shrimp larvae, comprising the following steps: transferring nauplii into a larval rearing pond for cultivation; after more than 80% of the nauplii have developed into zoea larvae, feeding them artificial feed for zoea larvae; after more than 80% of the zoea larvae have developed into mysid larvae, feeding them feed for Artemia nauplii and mysid larvae; after more than 80% of the mysid larvae have metamorphosed into shrimp larvae, feeding them rotifers and shrimp flakes; the artificial feed for zoea larvae includes: 45-55 parts of Schizochytrium powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil. The artificial feed for mysid larvae includes: 40-44 parts of shrimp flakes, 12-16 parts of tubifex worm powder, 5-10 parts of mannan oligosaccharide, 10-14 parts of scabra oil, and 20-30 parts of *Eupatorium fortunei* oil. This invention shortens the development time while obtaining high-quality P5 shrimp larvae, and throughout the entire cultivation process, the shrimp larvae mortality rate is low and the seedling survival rate is high.

The invention belongs to the technical field of microbial fermentation, and discloses a method for increasing the yield of schizochytrium limacinum palmitoleic acid through fermentation regulation and application, in the fermentation process of schizochytrium limacinum, one or more combined regulation strategies of controlling the fermentation temperature, optimizing the carbon nitrogen ratio of a fermentation culture medium and adjusting the pH value of fermentation liquor are adopted, and the yield of the schizochytrium limacinum palmitoleic acid is increased. And the metabolism flow direction of thalli is changed, so that the accumulation of palmitoleic acid C16: 1 in grease is improved. Compared with genetic engineering modification, the method disclosed by the invention does not need complex genetic manipulation and is high in safety; a 5L fermentation tank verifies that the proportion of palmitoleic acid in the grease can be remarkably increased to 16.12% from less than 1% of conventional fermentation, the grease yield is stable, and the method has good industrial application potential.

This application relates to a DNA structure for high expression of a target gene in microorganisms of the genus Schizochytrium, and a method for expressing a target gene in microorganisms of the genus Schizochytrium using the same. The DNA structure is characterized by including a 5' homology arm and a 3' homology arm that can be hybridized with high-expression sites of the genome of a microbial species Schizochytrium.

The invention relates to the technical field of pet food, in particular to bone joint maintenance type pet food and a preparation method thereof.The pet food is prepared from, by weight, 300-420 parts of protein sources, 110-320 parts of carbohydrates, 110-130 parts of fat, 75-85 parts of fibers and prebiotics and 95-105 parts of functional ingredients, hydrolyzed salmon protein, hermetia illucens powder, sweet potato powder, schizochytrium oil and the like are used as auxiliary materials, joint health compounds and vitamin minerals are used as auxiliary materials, and omega-3 fatty acid from schizochytrium is used for replacing traditional fish oil, so that the problem of sustainability is solved, the risk of heavy metal pollution is avoided, and meanwhile, the stability of the product is improved.

This invention relates to the field of aquatic feed technology, specifically to a largemouth bass and black soldier fly oil feed and its preparation method, which is made by mixing basic components, oil source components, additives and functional components. The basic components include the following ingredients in parts by weight: 30-40% fish meal, 3-8% soybean meal, 15-25% chicken meal, 3-8% corn gluten meal, 10-15% cottonseed protein, 4-10% wheat flour and 2-6% cassava starch. The oil source components include the following ingredients in parts by weight: 1-2% soybean oil, 2-5% black soldier fly larvae oil and 1-3% Schizochytrium oil. The functional components include the following ingredients in parts by weight: 0.5-1.5% premix, 0.5-1.0% choline chloride, 0.05-0.2% vitamin C, 0.1-1.0% DL-methionine and 0.05-0.2% ethoxyquin. The fish oil-free compound oil feed of the present invention can not only replace fish oil, but also supplement n-3 LC-UFA through Schizochytrium oil, which can improve the problem of insufficient deposition of unsaturated fatty acids in muscle and deterioration of muscle fiber structure caused by the use of black soldier fly larvae oil alone, thus improving both economic benefits and meat quality.

The invention provides a schizochytrium limacinum fermentation method which comprises the following steps: a hermetia illucens raw material is pretreated to enable the lauric acid content to be lower than 5%, and the pretreated hermetia illucens raw material can be used as a fermentation nitrogen source. It is found that the hermetia illucens raw material has the advantages of being high in protein content and low in cost and is a potential fermentation raw material, but lauric acid in the hermetia illucens has negative effects on fermentation of schizochytrium limacinum, the lauric acid is controlled within a certain range through a pretreatment means, the negative effects of the lauric acid on fermentation can be eliminated, and the fermentation efficiency is improved. Meanwhile, the chitin content is further reduced, the advantage of high protein content of the hermetia illucens raw material is more favorably played, the application way of the hermetia illucens powder is widened, the DHA fermentation production cost is reduced, and the industrial value is remarkable.

The invention discloses a preparation method, application and feeding method of a DHA laying hen feed additive based on fermented sea-buckthorn meat powder, fermentation components in the additive are formed by mixing and fermenting 35-45 parts of sea-buckthorn meat powder, 15-23 parts of fish meal and 34-45 parts of probiotics, and the probiotics comprise saccharomyces cerevisiae, saccharomyces cerevisiae boulardii, bacillus subtilis and enterococcus faecalis. Fermented hippophae rhamnoides meat powder and fish meal are used as main materials, probiotic fermentation can degrade antinutritional factors and optimize intestinal microecology, schizochytrium limacinum powder is matched to serve as an effective source of DHA, triglyceride type DHA contained in the schizochytrium limacinum powder can be efficiently absorbed and enriched in eggs after being decomposed by digestive tracts of laying hens, flaxseed oil provides a precursor, a multi-source DHA enrichment system is constructed, and the multi-source DHA enrichment system is used for improving the immunity of the laying hens. The bottleneck of single raw material conversion is broken through, and the DHA total amount of eggs is greatly increased.