53 results about "Schizochytrium" patented technology

The present application discloses a rumen-passed Schizochytrium preparation, a preparation method of the rumen-passed Schizochytrium preparation, and an application thereof. The rumen-passed Schizochytrium preparation comprises a core material and at least one layer of coating, wherein the core material comprises calcium alginate microspheres embedded with Schizochytrium cells, and the coating is coated on the core material so that the oil is wrapped in the calcium alginate microspheres, thereby improving the stability of unsaturated fatty acids and reducing the risk of unsaturated fatty acids leaking in the rumen; the preparation method comprises firstly subjecting the Schizochytrium fermentation product to calcium alginate microsphere embedding and immobilization treatment, then drying the immobilized fermentation product to obtain the core material, and finally coating the core material to obtain the rumen-passed Schizochytrium preparation, effectively avoiding the problems of "pipeline blockage" and "oxidation and deterioration of Schizochytrium powder", thereby improving the yield of Schizochytrium powder in the drying process and simplifying the preparation process, and the finally prepared rumen-passed Schizochytrium preparation has an ideal hardness.

The invention belongs to the field of microbial genetic engineering, and provides schizochytrium limacinum with high DHA content and low DPA content and application of the schizochytrium limacinum. According to the strain, an n3 front-end desaturase gene derived from crypthecodinium cohnii is integrated to a 18S rDNA site of schizochytrium limacinum ALS01, and omega-6 type DPA is converted into DHA by utilizing an extension-desaturation way. Under the optimized conditions of a 5L fermentation tank (the inoculation amount is 5-20%, the temperature is 25-28 DEG C, the ventilation ratio is 1.0-2.0 vvm, and fermentation is performed for 72-168 hours), the biomass is larger than or equal to 150g / L, the total grease is larger than or equal to 60%, the DHA total grease proportion is larger than or equal to 53%, the DPA total grease proportion is smaller than or equal to 2%, and the DHA yield is larger than or equal to 47.82 g / L. The invention solves the problems of unstable fish oil resource and insufficient DHA purity in the traditional DHA production, provides a new strain for the industrial production of high-purity DHA, and has important application value.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The present disclosure provides a Schizochytrium sp. Strain and its use thereof.

The present invention discloses a Schizochytrium strain that efficiently converts food waste to produce single-cell protein and its application, belonging to the field of bioengineering technology. The Schizochytrium spLOX‑2 deposit number is: CGMCC No.41623. The present invention also discloses a method for efficiently converting food waste to produce single-cell protein, fermenting food waste to produce volatile fatty acids; and using it as a carbon source to culture Schizochytrium to obtain single-cell protein. The recombinant strain spLOX‑2 in the present invention has improved utilization of lactic acid, and its biomass and protein yields are increased by 14.19% and 12% respectively compared with the wild-type strain, and the oil content is significantly increased by 107.1%. The present invention provides a new path for a sustainable bioeconomic model, in which waste is converted into valuable biochemicals, laying a solid foundation for expanding the scale of the process and the economic feasibility in industrial applications.

The present invention relates to: a feed composition for zooplankton, comprising biomass derived from Schizochytrium sp. microalgae; a method for culturing zooplankton using same; and an aquaculture method for fish or crustaceans. The biomass derived from Schizochytrium sp. microalgae of the present invention has the effect of nutritionally enriching zooplankton, and when zooplankton fed with the biomass is fed to fish or crustaceans, growth of fish or crustaceans is promoted and non-specific immunity is enhanced, and thus the biomass can be effectively used as a feed composition or a feed additive.

This invention discloses a highly absorbable, non-allergenic DHA brain-boosting oral liquid specifically designed for students of all ages, belonging to the field of functional nutritional beverage technology for teenagers. This invention uses high-purity Schizochytrium algae oil DHA and perilla seed active molecules as the core brain-boosting matrix, innovatively configuring a grade-specific ingredient system. The formula is customized for the physical condition and brain function needs of students at different grade levels: kindergarten, primary school, junior high school, high school, and university. Through a small-molecule cell-wall breaking extraction process, the nutrients are directly absorbed into the bloodstream and quickly converted into "brain gold" in the brain. This formula strictly avoids eight major food allergens, ensuring safety for all ages. It also specifically addresses sub-health issues related to learning across all grade levels, including early childhood brain development and emotional well-being, exam preparation efficiency and fatigue reduction for students preparing for higher education, and cognitive development and mental sustainability for university students. The product comes in standardized packaging of 50ml / vial and 15 vials / box, with a customized sweet taste tailored to different grade levels, making it highly acceptable to students. It has no side effects and is suitable for long-term healthy consumption by all school students, effectively improving brain efficiency and learning status across all grade levels, with broad market application prospects.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The present invention relates to a process for preparing a biomass of microorganisms of the genus Schizochytrium or Aurantiochytrium comprising the steps of: a) inoculating, in a bioreactor, a culture medium comprising a brine, preferably a 6% dry extract brine, and nitrogen, preferably at a concentration of between 5 and 15 g / L of culture medium, with an inoculum of microorganisms of the genus Schizochytrium or Aurantiochytrium, said brine being a co-product of the demineralization treatment of whey, b) culturing the microorganisms in said culture medium under conditions, in particular of temperature, pH, agitation and / or oxygen supply, appropriate for the growth of said microorganisms, preferably under non-axenic conditions, and c) collecting the biomass obtained at the end of step b).

This invention belongs to the field of microbial fermentation technology, specifically a process for producing EPA using Schizochytrium fungi as raw material. It employs a dual-gene modified strain with MAT gene overexpression and CLF gene precise knockout, which is domesticated under high-sugar oxidative stress to obtain a highly stable strain. Through three-stage temperature-controlled, stepwise dissolved oxygen fermentation, and the combined addition of para-aminobenzoic acid and ascorbic acid, EPA synthesis is enhanced while oxidative loss is reduced. Highly efficient EPA enrichment is achieved through low-temperature ultrasonic cell disruption of wet cells, urea inclusion, two-stage molecular distillation, and tandem purification using immobilized composite adsorption decolorization. This invention significantly improves EPA yield and proportion, reduces oxidative loss, and enhances product purity and stability through the synergistic effect of gene modification, metabolic regulation, and purification processes. The process is highly controllable, suitable for industrial production, and yields EPA products with high purity and good stability, which can be directly used in the food, health product, and pharmaceutical fields.

The application belongs to the technical field of microbial fermentation, and discloses a method for improving the production of palm oleic acid in Schizochytrium sp. by fermentation regulation and application. In the fermentation process of Schizochytrium sp., one or more combined regulation strategies of controlling the fermentation temperature, optimizing the carbon-nitrogen ratio of the fermentation medium and adjusting the pH value of the fermentation liquid are adopted to change the metabolic flow direction, so as to improve the accumulation of palm oleic acid C16:1 in oil. Compared with genetic engineering modification, the method does not need complex genetic operation and has high safety. Through verification of a 5L fermentation tank, the proportion of palm oleic acid in oil can be significantly increased from less than 1% in conventional fermentation to 16.12%, and the oil yield is stable, so that the method has good industrial application potential.

The invention belongs to the technical field of pomfret breeding, and particularly relates to wet compound feed for breeding juvenile pomfret and a preparation method, a feeding method and a device of the wet compound feed. The wet compound feed for breeding the juvenile pomfret is obtained by mixing powdery feed for the juvenile pomfret and water according to the mass ratio of 1: (1.2-1.5). The powdery feed for the juvenile pomfret is prepared from the following raw materials in parts by weight: 40-50 parts of white fish meal, 8-11 parts of mussel meat powder, 3-6 parts of enzymolysis squid paste, 6-9 parts of yolk powder, 4-8 parts of wheat protein powder, 10-17 parts of puffed corn flour, 4-7 parts of anchovy oil, 2-5 parts of schizochytrium limacinum powder, 0.4-1.0 part of a compound adhesive, 0.2-0.5 part of a compound enzyme preparation and 0.2-0.5 part of a compound microecological preparation. The feed is prepared from the following raw materials in parts by weight: 1.0-2.5 parts of a macroelement premix, 0.8-1.5 parts of a microelement premix and 0.4-1.0 part of a vitamin premix. The feed provided by the invention can solve the problems of low feeding rate, low survival rate and slow growth speed of the existing feed for feeding the pomfret in the prior art.

The invention discloses a microalgae composition and application thereof in fishing and farming integrated planting and breeding, and belongs to the technical field of aquaculture. The microalgae composition disclosed by the invention comprises chlorella, spirulina, nostoc, anabaena nitrogen fixation, schizochytrium limacinum and nannochloropsis oculata. The mass ratio of the chlorella to the spirulina to the nostoc to the nitrogen-fixing anabaena to the schizochytrium to the nannochloropsis oculata is 1: (4-5): (1: 1)-(2: 2.2-2.4): (3.4-3.6); the microalgae composition can be added into aquaculture feed to be applied to fishery and agricultural comprehensive planting and breeding; the fishing and farming integrated planting and breeding at least comprises rice-fish symbiosis; after the microalgae composition provided by the invention is applied to fishing and farming integrated planting, the growth of crops and aquaculture organisms for fishing and farming integrated planting can be effectively promoted.

The application provides a preparation method of a schizochytrium sp. protoplast. The preparation method of the schizochytrium sp. protoplast comprises the following steps: fermenting and culturing schizochytrium to obtain a fermentation liquor; a first fermentation stage: the temperature is 26-28 DEG C, and the culturing time is 36-40 h; a second fermentation stage: the temperature is 30-32 DEG C; and no sugar is additionally supplemented in the process of the fermentation culture. A large amount of protoplasts can be obtained by controlling the culturing conditions in the fermentation culture stage.

This invention belongs to the technical field of laying hen feed additives, specifically relating to a selenium-enriched, high-DHA, low-cholesterol functional egg feed additive and its preparation method. The additive is prepared by compounding selenized ginseng polysaccharide, Schizochytrium algae powder, rosemary extract, natural vitamin E, EGCG green tea extract, phytosterol micron powder, sodium butyrate, β-glucan, phytase, modified attapulgite clay, and maltodextrin in specific weight parts, followed by low-temperature premixing activation, antioxidant encapsulation treatment, low-temperature compound granulation, sieving, and packaging processes. This invention, through the synergistic effect of its components, enables eggs to reach stable functional indicators within 14 days of feeding. The yolk selenium content reaches 32.1~38.2 μg / 100g, DHA content reaches 210.5~261.7 mg / 100g, and cholesterol reduction rate reaches 16.7%~22.3%. Simultaneously, eggshell strength increases by 12.1%~18.5%, egg production rate remains at 95.1%~96.8%, and egg breakage rate decreases by 59.0%~72.0%. The product exhibits good stability, requires low dosage, and is easy to use, making it perfectly suited for large-scale egg-laying hen farming scenarios and possessing outstanding economic and health value.

The feed composition containing the microalgae-derived biomass of the present application does not cause any factors that reduce the quality of the feed, and can be usefully used in fish feed compositions using microalgae that are high in protein, fat, and minerals.

The invention relates to an efficient liquid submerged fermentation culture medium of russula vinosa polysaccharide and a fermentation method, and belongs to the technical field of fermentation. The formula of the efficient liquid submerged fermentation culture medium for russula vinosa polysaccharide comprises the following components: glucose, a russula vinosa stem water extract, a schizochytrium limacinum degreased powder water extract, a nannochloropsis oculata degreased powder protein enzymatic hydrolysate, an anoectochilus formosanus leaf water extract, a soybean cake powder protein enzymatic hydrolysate, wort, proteoglycan, monopotassium phosphate and magnesium sulfate heptahydrate. On the basis of the fermentation culture medium, a gradient fermentation technology is matched, efficient fermentation production of russula vinosa polysaccharide is achieved by adjusting the components and nutrient concentration of the culture medium in stages, the bottleneck of current russula vinosa polysaccharide resource acquisition is broken through, and industrial application of russula vinosa polysaccharide is promoted.

The present invention belongs to the field of breeding of special aquatic products, and particularly relates to a bionic nutrition - fortified microcapsule for jellyfish fry, its preparation method and application. The nutrition - fortified microcapsule is composed of a core and a wall. The core is composed of fish solubles powder, enzymatically hydrolyzed sea cucumber viscera powder, enzymatically hydrolyzed kelp powder, Antarctic krill powder, scallop skirt powder, chlorella powder, schizochytrium powder, haematococcus pluvialis powder, diatomite, taurine, yeast broken - wall powder, disodium nucleotide, chondroitin sulfate, compound vitamins, and sodium carboxymethylcellulose. The wall is composed of crab meat powder, artemia cysts powder, L - glutamic acid, chitosan, sodium alginate, calcium glycinate, enzymatically hydrolyzed soy protein, and whey protein powder. According to the feeding requirements of jellyfish fry, the present invention prepares ultra - fine nutrition microcapsules and granular nutrition microcapsules suitable for jellyfish fry of different specifications.

The invention discloses an artificial feed for fragrant snails containing a natural attractant. The artificial feed for fragrant snails contains chicken meat powder, Methylococcus capsulatus protein powder, Clostridium ethanolate protein powder and Candida utilis powder instead of existing proteins such as shrimp meat powder, soybean meal and fish meal. The artificial feed for fragrant snails is prepared by coordinating Nannochloropsis algae powder, Schizochytrium ruthenicum powder, soybean oil, chitosan oligosaccharide, microcrystalline cellulose, complex vitamins, complex minerals and a natural attractant prepared by mixing a protein hydrolysate of river mussels and a carbon dioxide supercritical extraction product of Ruditapes philippinarum. The artificial feed for fragrant snails can significantly improve the feeding behavior of fragrant snails and increase the food intake of fragrant snail larvae, promote the rapid growth of the conches in a relatively short period of time, and effectively solve the problems of the palatability of feed for fragrant snail larvae and the slow growth of artificial feed. More importantly, the artificial feed for fragrant snails can significantly improve the quality of conch meat, save breeding costs, reduce water pollution and improve breeding efficiency.

This invention relates to the field of aquaculture technology, and in particular to a biological attractant based on Schizochytrium fermentation broth and its preparation method; the biological attractant based on Schizochytrium fermentation broth is obtained by processing the fermentation broth after oil extraction from Schizochytrium; the fermentation broth after oil extraction from Schizochytrium is the residual fermentation broth after supercritical CO2 oil extraction or enzymatic hydrolysis oil extraction from Schizochytrium; the preparation method includes: pretreatment of fermentation broth, sterilization and stabilization treatment, concentration and regulation, and filling and storage; the three core components in the fermentation broth work synergistically, umami amino acids quickly trigger the taste perception of aquatic animals, algal polysaccharides slowly release odor molecules to prolong the feeding attraction time, and DHA enhances natural tropism, significantly improving the feeding induction rate of Litopenaeus vannamei, and maintaining a stable feeding attraction effect even in rainy and low-temperature environments; relying on the natural viscosity and structural characteristics of algal polysaccharides, there is no need to add stabilizers such as xanthan gum.

The application provides a method for fermenting astaxanthin. The method comprises fermenting astaxanthin by using a Schizochytrium sp. with a preservation number of CCTCC M 2021564 as a fermentation strain. The strain is a mutant strain with high astaxanthin content, which is obtained by using a Schizochytrium sp. screened and separated from seawater as a starting strain and by mutagenesis screening, and the astaxanthin obtained by the strain contains a large amount of DHA astaxanthin ester, and has high economic value and application value.

The invention provides a method for regulating microbial metabolism in the fermentation process of liquor yeast by using marine active peptides, which comprises the following steps: adding marine active peptides in the fermentation process of yeast in the production process of liquor, improving the growth and metabolism state of yeast fermentation microorganisms, optimizing the microbial community structure, promoting the formation of flavor substances and precursors thereof, and improving the yield of liquor yeast. The quality of the yeast and the contribution capability of the yeast to the white spirit flavor are improved; the marine active peptide is chlorella pyrenoidosa peptide, euphausia superba peptide, fish albumin peptide or schizochytrium limacinum peptide. Marine active peptides are introduced into a liquor yeast fermentation system, and different molecular weight intervals, amino acid compositions and peptide sequence structural characteristics of the marine active peptides are utilized, so that differential regulation and control of the yeast fermentation process are realized. Different types of marine active peptides can regulate the growth state and metabolic activity of microorganisms in the fermentation process, and influence the dynamic succession of microbial community structures in a fermentation system and the enrichment behavior of key functional bacteria.

The application discloses a screening marker recovery technology based on a Cre-LoxP combined Flp-Frt recombination system and application. The Cre-Loxp recombination system comprises a Cre recombinase and a LoxP site, and the LoxP nucleic acid combination comprises LoxP71 and LoxP66 sequences which can be specifically recognized and recombined by the Cre recombinase; similarly, the Flp-Frt recombination system comprises a Flp recombinase and a Frt site, and the Frt site comprises a Frt32 sequence which can be specifically recognized and recombined by the Cre recombinase. Due to the lack of screening markers and inducible promoters in the Schizochytrium, the site-specific recombinase cannot be recovered after being integrated into the genome, and therefore, the application aims to combine the two specific recombinase systems, so that the screening markers and the site-specific recombinase can be recovered unlimited times, and thus, the multi-gene editing of the Schizochytrium can be realized.

The invention discloses a fatty acid metabolism regulation gene mining method based on transcriptome data driving, a FindGene platform developed by the invention can efficiently integrate and standardize large-scale RNA-Seq data, systematic mining function related genes are analyzed through a weighted gene co-expression network, and the gene mining efficiency is improved. The limitation of dependence on small-scale experiments and manual screening in the past is broken through, and the efficiency and accuracy of key regulation gene screening are greatly improved. According to the schizosaccharomyces cerevisiae engineering strain QH-7 constructed by carrying out site-directed mutagenesis, overexpression and combined transformation on a key gene obtained by screening, the yield of fatty acid reaches 2.12 g / L and is increased by about 118.6% compared with that of a wild strain, and the biosynthesis efficiency of the fatty acid is greatly improved. According to the modified strain, the total fatty acid content is increased, meanwhile, the proportion of unsaturated fatty acids (C16: 1, C18: 1 and C18: 2) is remarkably increased, and the requirements for high-quality lipid raw materials in the fields of downstream biofuels, nutritional supplements and the like are met.

The invention discloses schizochytrium limacinum polypeptide with a lipid metabolism regulating effect as well as a separation and purification method and application. The separation and purification method comprises the following steps: S1, preparing a schizochytrium limacinum protein suspension; s2, adjusting the schizochytrium limacinum protein suspension obtained in the step S1 to be neutral, adding neutral protease for hydrolysis, cooling after boiling, and hydrolyzing to obtain schizochytrium limacinum protein hydrolysate; s3, the schizochytrium limacinum protein enzymatic hydrolysate obtained in the step S2 is filtered through a membrane, polypeptide with the molecular weight being smaller than 3 kDa and the amino acid sequence being SEQ ID NO.1 is obtained, and the schizochytrium limacinum polypeptide with the lipid metabolism adjusting effect is obtained. According to the invention, accumulation of triglyceride (TG) and cholesterol (TC) in cells is effectively reduced, lipotoxicity stress is relieved, and efficient and low-toxicity candidate active molecules are provided for development of drugs for resisting fatty liver, reducing blood fat or treating metabolic syndrome.

The application discloses a ylang-ylang flower composite repair factor, a preparation method and application, relates to the field of daily necessities, and contains the following components in mass fractions: ylang-ylang flower extract 1-10 parts, hydrolyzed keratin 0.1-5 parts, gynostemma pentaphyllum extract 0.5-8 parts, urea 0.1-3 parts, trehalose 0.5-6 parts, zinc PCA 0.1-3 parts and schizochytrium fermentum filtrate 10-50 parts; wherein the ylang-ylang flower extract is extractum obtained by alcohol extraction and ethyl acetate extraction of ylang-ylang flowers, and the total terpene content is not less than 15% in terms of drimane D; the molecular weight of the hydrolyzed keratin is 500-3000 Da; the ylang-ylang flower composite repair factor, the preparation method and the application construct a ylang-ylang flower extract plus zinc PCA bidirectional oil control channel, and inhibit excessive secretion of sebum from both lipid synthesis in sebaceous gland cells and hormone signals.

The invention discloses deep moisturizing and hydrating gel as well as a preparation method and application of the deep moisturizing and hydrating gel. The composition comprises the following components: bifidus yeast fermentation product filtrate, human-like collagen, hydrolyzed fish collagen peptide, alpha-arbutin, crithmum maritimum callus culture filtrate, eryngium maritimum callus culture filtrate, desert rose callus culture filtrate, peony fermentation filtrate, hydrolyzed placenta (sheep) extract, leukocyte extract and soybean fermentation metabolite. The skin care product is prepared from a brown algae extract, 1, 3-butanediol, sodium hyaluronate, D-panthenol, an aloe extracting solution, allantoin, carbomer, xanthan gum, cyclopentasiloxane (and) cyclohexasiloxane, polyacrylic acid (ester) copolymer sodium (and) lecithin, a hamamelis mollis distillate, a jania rubens extract, arginine, sweet orange essential oil and the balance of water. The anti-wrinkle gel has the advantages that the anti-wrinkle gel is mild and non-irritant, multiple effects of relieving, moisturizing, tightening and anti-wrinkle are synergistic, the gel is not easy to flow, the dosage is convenient to control, no sticky feeling exists, and the experience feeling is good.

The invention belongs to the technical field of aquaculture. The invention discloses a method for regulating and controlling nutrient enrichment of initial feed of aquatic products based on lipid metabolism. The method is characterized by comprising the following steps: 1) key fatty acid identification: respectively determining fatty acid compositions of target aquatic product larvae and fairy shrimp nauplii, and identifying unsaturated fatty acids which are relatively lacking in the fairy shrimp nauplii but have relatively high requirements on the target aquatic product larvae; (2) inspection of a strengthening source: detecting schizochytrium limacinum powder which is identified in the step (1) and lacks unsaturated fatty acid, and verifying the content of target unsaturated fatty acid in the schizochytrium limacinum powder; 3) bait nutrition enhancement: using schizochytrium limacinum powder as an enhancer for feeding fairy shrimp nauplii, and increasing the unsaturated fatty acid content of target aquatic product larvae; (4) intensified bait feeding and effect evaluation: feeding the nutrient-intensified fairy shrimp nauplii to the target aquatic product larvae, and evaluating the growth performance of the target aquatic product larvae and the nutrition improvement condition of muscular tissues. The method can effectively improve the nutritional value of the initial feed of the aquatic product.

The invention discloses a preparation method of an artificial feed for breeding hermetia illucens larvae as well as a product and application of the artificial feed, schizochytrium limacinum algae residues are added into a basic feed raw material, and the artificial feed for breeding the hermetia illucens larvae is obtained through fermentation of bacillus subtilis. Bacillus with lipase activity is added to perform enzymolysis on schizochytrium limacinum residues, so that the problem that artificial feed is thick due to addition of the schizochytrium limacinum residues is solved, and easy-to-absorb algae mud components rich in nutrients such as micromolecular fatty acid and the like are obtained; the feed additive is added into an artificial feed for black soldier fly larvae as a nutrition enhancer so as to achieve the purpose of improving the growth and breeding performance of larvae. The raw materials are derived from schizochytrium limacinum production waste residues, the cost is low, waste utilization is achieved, the related technology facilitates rapid and healthy breeding of larvae in the industrial hermetia illucens breeding process, and high-quality larvae can be provided for spawning of rear-end adults.