133 results about "Schizochytrium" patented technology

The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and / or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and / or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. In an embodiment, lipids are produced by growing microorganisms of the order Thraustochytriales in a medium and extracting the lipids.

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g / L and DHA product up to 8.27 g / L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

The invention relates to a method for producing triglyceride type docosahexenoic acid fat by industrially fermenting schizochytrium. The method comprises the following step: the docosahexenoic acid fat is prepared by fermenting the schizochytrium taken as the strain with the a culture medium with microelements; wherein the culture medium with the microelements comprises a carbon source, a nitrogen source, inorganic salt and microelements, and the microelements comprise at least one of biotin, chlorophyll, beta-carotene, vitaminB1, vitaminB6 and vitaminB12. The prepared docosahexenoic acid fat does not need to be further chemically modified and can be directly used by people needing DHA, including infants, pregnant women, breast feeding women or patients who suffer from diseases due to insufficient DHA and need to supplement DHA. The invention has the advantages of high DHA content, high purity, safe and reliable quality, low production cost, stable production and almost no EPA; and the environmental-friendly, safe and high-content triglyceride type DHA fat can be produced with low cost at a large scale.

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. In an embodiment, lipids are produced by growing microorganisms of the order Thraustochytriales in a medium and extracting the lipids.

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

A process is provided for growing microflora of the order Thraustochytriales such as Thraustochytrium, Schizochytrium, and mixtures thereof, in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment, the process produce, microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further provided is a food product which includes Thraustochytrium, Schizochytrium, and mixture thereof, and a component selected from flaxseed, reapedeed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. A method for increasing omega-3 highly unsaturated fatty acid content of eggs by feeding a feed to poultry. The feed contains the microorganisms of the order Thraustochytriales having a omega-3 highly unsaturated fatty acid content of more than 6.7 percent of total cell dry weight. The microorganisms include strains of Schizochytrium and Thraustochytrium such as ATCC No.(s) 20888 and 20889, and ATCC No.(s) 20890, 20891 and 20892, respectively. Also provided are eggs containing an increased amount of these fatty acids.

The invention discloses a method for extracting and refining docosahexaenoic acid from schizochytrium, which is characterized by comprising the following steps: carrying out flocculation processing ofschizochytrium ATCC 20888 fermentation liquid, then carrying out solid-liquid separation, mechanically crushing cells after alkali cell wall is broken, and extracting crushed thallus by adopting organic solvents to obtain DHA rough oil; processing the DHA rough oil by adopting the steps of degumming, alkali refining, decoloring and deodorizing to obtain DHA essential oil. The invention is simplein operation, can reduce the extraction cost of the DHA oil, only adopts the organic solvents during the operating process, has no residues finally, improves the quality of the DHA oil after being refined, has the DHA content in the extract about 28-51 percent and is suitable for large-scale production of DHA.

The invention discloses a method for extracting grease from schizochytrium. The method comprises the following steps: (1) adjusting the pH (Potential of Hydrogen) of the fermentation broth of the schizochytrium until reaching an acid stage, and then adding an organic solvent to stir, so as to realize the wall breaking of schizochytrium cells and extraction of microalgae oil; (2) adding a flocculant to the fermentation broth obtained in step (1) to flocculate, and extracting by the organic solvent to obtain crude algae oil; and (3) treating the crude algae oil by degumming, discoloring, and deacidifying / deodorizing, so as to obtain the microalgae oil rich in DHA (Docosahexaenoic Acid). According to the method disclosed by the invention, autolysis wall-breaking technology is adopted, so that the technological process in wall breaking and extraction of the schizochytrium can be simplified, the operation time in wall breaking can be shortened, the investment on equipment is decreased, and the cost is lowered; and the biological flocculant flocculation technology is carried out, therefore, the demulsification technological process is simplified, the time for layering is shortened, the layering effect is improved, the load due to wastewater discharge and water treatment caused by the demulsification process can be avoided, and the cost of water treatment can be lowered.

The invention provides schizochytrium LX0809 (marine fungus), which has a high docosahexaenoic acid (DHA) production capability. The schizochytrium LX0809 of the invention was separated from the corroded soil collected from the red beach in Zhaoquanhe Town, Dawa County, Liaoning Province and was preserved in China General Microbiological Culture Collection Center with a preservation number CGMCC No.3535 on December 23th, 2009. When the schizochytrium LX0809 is used for fermenting supplementary food, the dry cell weight of the fermentation liquor can reach 72.5 g / L, and the DHA content can reach 15.3g / L. LX 0809 thallus cells and extracts thereof can be used as food additives for edible oil, dairy products, grain products and the like and can be used as a feed additive for aquaculture and livestock culture and the like. Saturated triglycercide in the thallus cells can be used to produce biodiesel through catalytic transesterification.

The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.

The invention discloses a compound nutritious feed for shrimps, which is prepared from soybean waste, fish meal, dephenolized cotton seed protein, double-low rape seed meal, cuttle-bone powder, shrimp head powder, green sea weed powder, soybean lecithin powder, soybean oil, fish oil, calcium dihydrogenphosphate, flour, glutelin powder, husked powder, microelement additive for shrimps, composite vitamins for shrimps, 50% choline chloride, schizomycete, bacillus licheniformis, high activity phytase, beta-gluglucosan and polyxylose.

This invention discloses Schizochytrium WZU477 and its application in preparing DHA powder and DHA grease. The Schizochytrium WZU477 is obtained by gathering rotten leaves and using pine pollen as the bait, and is preserved at China General Microbiological Culture Collection Center (CGMCC No. 1730). The Schizochytrium WZU477 can produce fermented liquid that has 42.5 g / L cell dry weight and 14.1 g / L DHA acid after cultured for 5 d under optimal conditions. The productivity of DHA is 2.76 g / L .d, twice that of previously reported DHA production.

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g / L and DHA product up to 8.27 g / L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

The invention discloses a method for cultivating high-yield DHA (docosahexaenoic acid) through schizochytrium, and belongs to the field of microbial fermentation. The method comprises steps as follows: a schizochytrium seed solution with the dry cell weight being 18-22 g / L after culturing in a shake flask is inoculated into a seed tank and then inoculated into a fermentation tank after being cultured to reach an inoculation transfer level, high-density culture is performed at the high temperature being 22 DEG C-33 DEG C in an early stage, induction culture is performed in a threatening manner for 110-130 hours at the low temperature being 18 DEG C-22 DEG C in a late stage, and glucose and total nitrogen are kept in a certain concentration and pH (potential of hydrogen) is kept ranging from 5.5 to 6.6 through feeding of a glucose solution, a nitrogen source solution and an alkaline solution in the fermentation process. The dry cell weight of a fermentation solution obtained with the method reaches 120g / L-150 g / L, the density of the DHA reaches 40g / L-50 g / L and is 1.2-1.5 times higher than reported DHA productivity, and the method is very suitable for industrial production of DHA.

The invention relates to the field of microbial fermentation engineering, and concretely relates to aurantiochytrium sp. and application. Aurantiochytrium sp. has the classification name of aurantiochytrium sp. SD006, is preserved in China General Microbiological Culture Collection Center, at No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City since 21st, October, 2013, and the preservation number is CGMCC NO. 8383. Aurantiochytrium sp. is applicable to prepare docosahexenoic acid. The obtained bacterial strain has fast growth speed and short fermentation time. Docosahexenoic acid can be produced by utilizing high-density fermentation of aurantiochytrium sp. strains. Under the fermentation conditions shown in the specification, a maximum yield can be obtained in 60-70 h. After fermentation is performed for 70 h, the biomass obtained is 180-200 g / L, and the DHA output is 50-65 g / L.

The invention relates to a method for producing triglyceride type docosahexenoic acid fat by industrially fermenting schizochytrium. The method comprises the following step: the docosahexenoic acid fat is prepared by fermenting the schizochytrium taken as the strain with the a culture medium with microelements; wherein the culture medium with the microelements comprises a carbon source, a nitrogen source, inorganic salt and microelements, and the microelements comprise at least one of biotin, chlorophyll, beta-carotene, vitaminB1, vitaminB6 and vitaminB12. The prepared docosahexenoic acid fat does not need to be further chemically modified and can be directly used by people needing DHA, including infants, pregnant women, breast feeding women or patients who suffer from diseases due to insufficient DHA and need to supplement DHA. The invention has the advantages of high DHA content, high purity, safe and reliable quality, low production cost, stable production and almost no EPA; and the environmental-friendly, safe and high-content triglyceride type DHA fat can be produced with low cost at a large scale.

The present invention relates to a method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists and more particularly to a method of enhancing levels of docosahexaenoic acid and eicosapentaenoic acid in cells of thraustochytrid protist belonging to the genera Schizochytrium, Thraustochytrium and Aplanochytrium deposited at The Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India under the accession numbers MTCC 5121, MTCC 5122 and MTCC 5123 respectively by growing the same in a medium with increased viscosity, whereby the cells thus enriched in the said polyunsaturated fatty acids (PUFAs) can then be utilized successfully in various beneficial applications that require polyunsaturated fatty acids, such as in animal feeds, human nutrition and extraction of the PUFAs for nutritional supplementation.

The present invention relates to various methods to produce a variety of omega-3 fatty acids from various species of alga using crude glycerol as a substrate for algal growth. In one embodiment, the present invention relates to various methods to produced docosahexaenoic acid (DHA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and / or Labyrinthulea species of alga. In another embodiment, the present invention relates to various methods to produced eicosapentaenoic acid (EPA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and / or Labyrinthulea species of alga. In one instance, the methods of the present invention utilize crude glycerol as at least a portion of the culture medium for the various micro-organisms disclosed herein to enable the production of one or more omega-3 fatty acids. In one embodiment, the crude glycerol of the present invention can be generated from a biodiesel process as a substrate for the production of either docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention discloses a novel strain Aurantiochytrium sp. YLH70 and an application Aurantiochytrium sp. YLH70 for synthesizing DHA. The raw materials which are non-grain resource Jerusalem artichoke and plant starch sourced high fructose corn syrup are great in output and low in price, so that the production cost of DHA can be lowered; Jerusalem artichoke hydrolysate and high fructose corn syrup contain high concentration reducing sugar which is mainly sugars such as fructose, fructo-oligose and glucose which are easy to ferment, becomes a potential carbon source for producing DHA from Aurantiochytrium sp.; the fermenting biomass of the Aurantiochytrium sp. YLH70 strain with an excellent fermenting performance can reach 54.61-76.52g / L in a culture medium which takes the Jerusalem artichoke hydrolysate as a unique carbon source and the DHA output can reach 12.56-18-78g / L; in a culture medium which takes high fructose corn syrup as a unique carbon source, the fermenting biomass can reach 70.51-79.85g / L and the DHA output can reach 14.1-20.1g / L.

The invention discloses a method for cultivating schizochytrium oil-producing grease employing schizochytrium and crude glycerine, and belongs to the technical field of microorganisms. The schizochytrium (Schizochytrium sp.) 691Y01 is preserved at the China General Microbiological Culture Collection Center; the accession number of the preservation center is CGMCC NO.9043; and the bacterial strain is obtained by ultraviolet mutagenesis of schizochytrium (Schizochytrium sp.) WZU6961. Compared with the Schizochytrium sp. WZU6961, the palmitic acid content is improved by 50% while the DHA is reduced by 30%; rapid growth and accumulation of grease are achieved by biodiesel byproduct crude glycerine as a carbon source; and grease components are relatively suitable for production of the biodiesel.

The invention provides a fermentation production technique of schizogenesis chytrid, which solves the problems of lower yield and higher production cost in the prior art. The fermentation production technique has the technical scheme that bean pulp is taken as main nitrogen source for fermentation production, and the technique comprises the steps of bean pulp hydrolysis, pressing filter, preparation of fermentation medium, arrangement of a fermentation tank system, collection and drying of the schizogenesis chytrid, and preparing the schizogenesis chytrid dry powder. The new fermentation production technique of the schizogenesis chytrid takes the bean pulp as main nitrogen, thus not only reducing the cost of fermentation production, but also improving the growth rate of the schizogenesis chytrid and promoting fatty acid accumulation of the cells of the schizogenesis chytrid.

Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and / or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and / or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

The invention relates to a production method and application of an aquatic feed. Specifically, the invention relates to a preparation method for dimethylpropiothetin, a production method for a feed or a method for improving the flavor of fermented soybean meal. The method is characterized by comprising a step of fermenting soybean meal by using bacillus microbes and Schizochytrium. The invention also provides a fermented soybean meal which is prepared through fermentation via the bacillus microbes and Schizochytrium according to the method. The invention further provides a feed containing the fermented soybean meal. As the method is used for fermentation of soybean meal, anti-nutrition factors in the soybean meal are degraded, the content of small peptide is increased, and the produced fermented soybean meal has special flavor and can increase the food intake and weight gain rate of aquatic animals, reduce a feed coefficient and improve breeding benefits.

The invention discloses a DHA regulating agent and a chicken feed applying the DHA regulating agent. The DHA regulating agent comprises the following ingredients in parts by weight: 3-5 parts of hazelnut powder, 5-20 parts of peanut powder, 8-30 parts of sesame, 8-25 parts of almond powder, 12-40 parts of schizochytrium powder, 14-23 parts of sunflower seed and 3-15 parts of flaxseed. The DHA regulating agent and the chicken feed applying the DHA regulating agent have the advantages that DHA content in eggs can be increased, quality of the eggs also can be improved, the DHA regulating agent in the whole production process is safe and healthy, no antibiotic or hormone is contained, no heavy metal pollution is generated, and blank in a domestic technology is filled up.