133 results about "Schizochytrium" patented technology

The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and/or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and/or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g/L and DHA product up to 8.27 g/L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

A process is provided for growing microflora of the order Thraustochytriales such as Thraustochytrium, Schizochytrium, and mixtures thereof, in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment, the process produce, microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further provided is a food product which includes Thraustochytrium, Schizochytrium, and mixture thereof, and a component selected from flaxseed, reapedeed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. A method for increasing omega-3 highly unsaturated fatty acid content of eggs by feeding a feed to poultry. The feed contains the microorganisms of the order Thraustochytriales having a omega-3 highly unsaturated fatty acid content of more than 6.7 percent of total cell dry weight. The microorganisms include strains of Schizochytrium and Thraustochytrium such as ATCC No.(s) 20888 and 20889, and ATCC No.(s) 20890, 20891 and 20892, respectively. Also provided are eggs containing an increased amount of these fatty acids.

The invention discloses a method for extracting and refining docosahexaenoic acid from schizochytrium, which is characterized by comprising the following steps: carrying out flocculation processing ofschizochytrium ATCC 20888 fermentation liquid, then carrying out solid-liquid separation, mechanically crushing cells after alkali cell wall is broken, and extracting crushed thallus by adopting organic solvents to obtain DHA rough oil; processing the DHA rough oil by adopting the steps of degumming, alkali refining, decoloring and deodorizing to obtain DHA essential oil. The invention is simplein operation, can reduce the extraction cost of the DHA oil, only adopts the organic solvents during the operating process, has no residues finally, improves the quality of the DHA oil after being refined, has the DHA content in the extract about 28-51 percent and is suitable for large-scale production of DHA.

The invention provides schizochytrium LX0809 (marine fungus), which has a high docosahexaenoic acid (DHA) production capability. The schizochytrium LX0809 of the invention was separated from the corroded soil collected from the red beach in Zhaoquanhe Town, Dawa County, Liaoning Province and was preserved in China General Microbiological Culture Collection Center with a preservation number CGMCC No.3535 on December 23th, 2009. When the schizochytrium LX0809 is used for fermenting supplementary food, the dry cell weight of the fermentation liquor can reach 72.5 g/L, and the DHA content can reach 15.3g/L. LX 0809 thallus cells and extracts thereof can be used as food additives for edible oil, dairy products, grain products and the like and can be used as a feed additive for aquaculture and livestock culture and the like. Saturated triglycercide in the thallus cells can be used to produce biodiesel through catalytic transesterification.

The invention discloses a compound nutritious feed for shrimps, which is prepared from soybean waste, fish meal, dephenolized cotton seed protein, double-low rape seed meal, cuttle-bone powder, shrimp head powder, green sea weed powder, soybean lecithin powder, soybean oil, fish oil, calcium dihydrogenphosphate, flour, glutelin powder, husked powder, microelement additive for shrimps, composite vitamins for shrimps, 50% choline chloride, schizomycete, bacillus licheniformis, high activity phytase, beta-gluglucosan and polyxylose.

The invention discloses a method for cultivating high-yield DHA (docosahexaenoic acid) through schizochytrium, and belongs to the field of microbial fermentation. The method comprises steps as follows: a schizochytrium seed solution with the dry cell weight being 18-22 g/L after culturing in a shake flask is inoculated into a seed tank and then inoculated into a fermentation tank after being cultured to reach an inoculation transfer level, high-density culture is performed at the high temperature being 22 DEG C-33 DEG C in an early stage, induction culture is performed in a threatening manner for 110-130 hours at the low temperature being 18 DEG C-22 DEG C in a late stage, and glucose and total nitrogen are kept in a certain concentration and pH (potential of hydrogen) is kept ranging from 5.5 to 6.6 through feeding of a glucose solution, a nitrogen source solution and an alkaline solution in the fermentation process. The dry cell weight of a fermentation solution obtained with the method reaches 120g/L-150 g/L, the density of the DHA reaches 40g/L-50 g/L and is 1.2-1.5 times higher than reported DHA productivity, and the method is very suitable for industrial production of DHA.

The invention relates to the field of microbial fermentation engineering, and concretely relates to aurantiochytrium sp. and application. Aurantiochytrium sp. has the classification name of aurantiochytrium sp. SD006, is preserved in China General Microbiological Culture Collection Center, at No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City since 21st, October, 2013, and the preservation number is CGMCC NO. 8383. Aurantiochytrium sp. is applicable to prepare docosahexenoic acid. The obtained bacterial strain has fast growth speed and short fermentation time. Docosahexenoic acid can be produced by utilizing high-density fermentation of aurantiochytrium sp. strains. Under the fermentation conditions shown in the specification, a maximum yield can be obtained in 60-70 h. After fermentation is performed for 70 h, the biomass obtained is 180-200 g/L, and the DHA output is 50-65 g/L.

The present invention relates to a method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists and more particularly to a method of enhancing levels of docosahexaenoic acid and eicosapentaenoic acid in cells of thraustochytrid protist belonging to the genera Schizochytrium, Thraustochytrium and Aplanochytrium deposited at The Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India under the accession numbers MTCC 5121, MTCC 5122 and MTCC 5123 respectively by growing the same in a medium with increased viscosity, whereby the cells thus enriched in the said polyunsaturated fatty acids (PUFAs) can then be utilized successfully in various beneficial applications that require polyunsaturated fatty acids, such as in animal feeds, human nutrition and extraction of the PUFAs for nutritional supplementation.

The present invention relates to various methods to produce a variety of omega-3 fatty acids from various species of alga using crude glycerol as a substrate for algal growth. In one embodiment, the present invention relates to various methods to produced docosahexaenoic acid (DHA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and/or Labyrinthulea species of alga. In another embodiment, the present invention relates to various methods to produced eicosapentaenoic acid (EPA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and/or Labyrinthulea species of alga. In one instance, the methods of the present invention utilize crude glycerol as at least a portion of the culture medium for the various micro-organisms disclosed herein to enable the production of one or more omega-3 fatty acids. In one embodiment, the crude glycerol of the present invention can be generated from a biodiesel process as a substrate for the production of either docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention discloses a method for cultivating schizochytrium oil-producing grease employing schizochytrium and crude glycerine, and belongs to the technical field of microorganisms. The schizochytrium (Schizochytrium sp.) 691Y01 is preserved at the China General Microbiological Culture Collection Center; the accession number of the preservation center is CGMCC NO.9043; and the bacterial strain is obtained by ultraviolet mutagenesis of schizochytrium (Schizochytrium sp.) WZU6961. Compared with the Schizochytrium sp. WZU6961, the palmitic acid content is improved by 50% while the DHA is reduced by 30%; rapid growth and accumulation of grease are achieved by biodiesel byproduct crude glycerine as a carbon source; and grease components are relatively suitable for production of the biodiesel.

The invention relates to a production method and application of an aquatic feed. Specifically, the invention relates to a preparation method for dimethylpropiothetin, a production method for a feed or a method for improving the flavor of fermented soybean meal. The method is characterized by comprising a step of fermenting soybean meal by using bacillus microbes and Schizochytrium. The invention also provides a fermented soybean meal which is prepared through fermentation via the bacillus microbes and Schizochytrium according to the method. The invention further provides a feed containing the fermented soybean meal. As the method is used for fermentation of soybean meal, anti-nutrition factors in the soybean meal are degraded, the content of small peptide is increased, and the produced fermented soybean meal has special flavor and can increase the food intake and weight gain rate of aquatic animals, reduce a feed coefficient and improve breeding benefits.