842 results about "Carotene" patented technology

The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.

The invention belongs to the technical field of hyperspectral imaging and machine learning, and discloses a plant carotenoid rapid determination method based on hyperspectrum and machine learning. The measuring system comprises a hyperspectral imaging device, a spectrum preprocessing module, a characteristic wavelength extraction module, a machine learning modeling module and a carotenoid content prediction module which are sequentially connected through a data processing unit. According to the method, spectral data of a plant sample is obtained in combination with a hyperspectral imaging technology, key wavelengths are screened through spectrum preprocessing and a feature extraction algorithm, and a carotenoid content prediction model is established by using machine learning models such as random forest regression, partial least squares regression or a gradient elevator. According to the method, rapid and nondestructive detection of the plant carotenoid can be realized without a chemical reagent, the determination precision and efficiency are improved, and the defects of complex operation, long time consumption and strong destructiveness of a traditional detection method are overcome.

The invention relates to the technical field of microorganisms, and provides heteromorpha sp. RXH-HY13 and application of the heteromorpha sp. RXH-HY13. The invention provides a dysococcus globosum RXH-HY13, which is preserved in the China Center for Type Culture Collection on July 11, 2024, the preservation number of the dysococcus globosum RXH-HY13 is CCTCC NO: M 20241535, and the preservation number of the dysococcus globosum RXH-HY13 is CCTCC NO: M 20241535. The invention also provides an application of the special-shaped algae RXH-HY13. The special-shaped algae RXH-HY13 is used for enriching at least one of organic selenium and carotenoid in a selenium ion stress environment; the invention also provides another application of the heteromorphic algae RXH-HY13. The application comprises the step that the heteromorphic algae RXH-HY13 is used for preparing a product rich in at least one of organic selenium and carotenoid. The special-shaped algae RXH-HY13 provided by the invention has certain tolerance to a selenium ion stress environment, so that the special-shaped algae RXH-HY13 not only can enrich organic selenium in the selenium ion stress environment, but also can promote accumulation of various carotenoids, and has the effect of synergistically enriching the organic selenium and the various carotenoids.

The invention discloses a recombinant neurospora crassicola strain for producing astaxanthin as well as a construction method and application of the recombinant neurospora crassicola strain. The recombinant neurospora crassa contains key enzymes beta-carotene ketolase and beta-carotene hydroxylase in a heterologous astaxanthin synthetic pathway, and through pathway expression of a targeted subcellular compartment, crosstalk among metabolites is effectively prevented, metabolic flux of a biosynthetic pathway is coordinated, and the activity of the neurospora crassa is improved. The effective contact between the heterologous protein and the substrate in the astaxanthin synthetic route is promoted, the sporulation capability of the heterologous protein is improved, the astaxanthin production and storage capability of the neurospora crassiforme is effectively improved, and the method has relatively high popularization and application values.

The invention belongs to the technical field of biology, and particularly relates to a method for cultivating butter crabs. According to the method, the growth process of the blue crabs to the butter crabs is regulated and controlled through the indoor environment factors, the butter crabs can be effectively cultured by means of the high efficiency, convenience and low cost of regulation and control of the indoor environment factors, and the method can be suitable for large-scale batch production of the butter crabs all the year round. According to the method, the butter crabs can be cultivated within a very short time (only 15-20 days), the conversion rate is high, particularly, the number of the obtained high-grade butter crabs is larger than that of the butter crabs obtained through a conventional method, the color of abdominal navel is orange yellow, the color of articular membrane (yellow blue value) can reach 37, the content of hemolymph fat can reach 0.22 g / 100g, the content of hemolymph beta carotene can reach 396 micrograms / 100g, and the content of the butter crabs can reach 0.12 micrograms / 100g. The edible value and the economic value are extremely high.

The invention discloses an apple anthracnose leaf blight recognition five-dimensional data fusion method based on a patrol robot, relates to the technical field of anthracnose leaf blight recognition, and solves the problems of limited sensing dimension, large illumination interference and inaccurate spatial positioning in the prior art. Comprising the following steps: S1, acquiring surface curvature characteristics of apple leaves, collecting RGB-D images of the leaves, constructing a three-dimensional topological structure of the leaves, and positioning scab space distribution; s2, acquiring spectral reflection information of apple leaves, quantifying biochemical components of chlorophyll a and carotenoid, and constructing a vegetation index feature set sensitive to diseases; s3, apple tree canopy temperature information is collected, a transpiration anomaly detection model is constructed, and multi-modal fusion of thermal infrared and point cloud data is realized; and S4, integrating spatial distribution, spectral reflection and thermal radiation information, constructing a five-dimensional phenotypic characteristic matrix model, and comprehensively analyzing apple phenotypic characteristics. According to the method, the recognition accuracy under the conditions of blade shielding, uneven illumination, blade posture change and environment interference can be remarkably improved.

The invention discloses pseudomonas TB392 and application thereof in degradation of pectin and generation of flavor substances in tobacco leaves, and belongs to the technical field of biology. The strain is preserved in the China General Microbiological Culture Collection Center on October 16, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32230. The strain can produce pectinase at high yield, has the capability of reducing nicotine, can degrade macromolecular compounds such as carotenoid, starch, pectin, cellulose and the like in tobacco leaves, and can remarkably improve the content of flavor substances in the tobacco leaves. After the strain or the prepared microbial agent is applied to tobacco leaves, the quality of the tobacco leaves can be improved, and the use value of the tobacco leaves is improved.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

The invention relates to the technical field of daily chemicals, in particular to a white tomato whitening and repairing composition as well as a preparation method and application thereof. The composition comprises the following components in percentage by mass: 68-85% of jojoba oil, 10-25% of squalane and the balance of white tomato extract, wherein the total percentage is 100%. The white tomatoes are rich in colorless carotenoids (such as phytoene and tetrahydrolycopene), and have excellent antioxidant and whitening capabilities: melanin formation is inhibited, and the colorless carotenoids can inhibit tyrosinase activity, reduce melanin generation and fundamentally whiten the skin. Pigmentation caused by ultraviolet rays can be relieved through the anti-oxidation effect, and the color spots are prevented and faded. The skin color can be brightened after continuous use, so that the skin presents natural luster.

The invention relates to the technical field of feed formulas, and particularly discloses a natural mixed feed additive and a preparation method thereof. The natural mixed feed additive comprises the following raw materials in parts by mass: 4-7 parts of vitamin E, 5-15 parts of beta-carotene, 10-16 parts of soybean oil, 20-35 parts of deionized water, 5-8 parts of modified probiotic microcapsules and 1-5 parts of an emulsifier, wherein the modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics; the preparation method comprises the following steps: heating 10-16 parts of soybean oil to 35-45 DEG C, then adding 4-7 parts of vitamin E and 5-15 parts of beta-carotene, and stirring; adding 1-5 parts of an emulsifier into 20-35 parts of deionized water, heating to 45-55 DEG C, stirring, and then slowly adding into the mixed solution obtained in the step S1; 5-8 parts of modified probiotic microcapsules are added, and stirring is conducted for 8-12 min under the condition of 100-200 r / min. The natural mixed feed additive disclosed by the invention has the advantage that the defect that the feed additive still has defects in the aspect of reducing intestinal diseases of animals can be overcome.

The invention relates to the technical field of microbial fermentation, and discloses application of a regulatory factor in promoting schizochytrium limacinum to produce DHA (docosahexaenoic acid), and the regulatory factor contains beta-carotene and reduced glutathione. In addition, the invention also provides a method for improving DHA production of schizochytrium limacinum and a preparation method of microbial oil. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium, and adding beta-carotene and reduced glutathione into the culture medium in the fermentation process; in the culture medium, the content of the beta-carotene is 1.5 to 2g / L. The beta-carotene and the reduced glutathione can effectively improve the content of grease and DHA prepared by fermentation of schizochytrium limacinum, the content of DHA can reach 63.9%, and the beta-carotene and the reduced glutathione are non-toxic and high in safety.

The invention discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application of the nucleic acid aptamer OUC-Fx11-5T, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthin in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-5T and the fucoxanthine is determined to be 68.2 nM through an isothermal titer thermal method, the nucleic acid aptamer OUC-Fx11-5T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to the fucoxanthine, and can be used for preparing the fucoxanthine aptamer OUC-Fx11-5T of the fucoxanthine. The method can be used for identification or detection of fucoxanthine.

Provided herein are pharmaceutical compositions comprising carotenoids, including liposomes that encapsulate carotenoids including ionizable carotenoids such as trans-crocetin. The provided compositions have uses in treating diseases, disorders and conditions associated with, but not limited to, infection, endotoxemia, inflammation, sepsis, ischemia, hypoxia, shock, stroke, lung injury, wound healing, traumatic injury, reperfusion injury, cardiovascular disease, kidney disease, liver disease, inflammatory disease, metabolic disease, pulmonary disorders, blood related disorders and hyperproliferative diseases such as cancer. Methods of making, delivering, and using the pharmaceutical compositions are also provided.

The invention discloses a formula and a preparation method of milk skin yoghurt. The milk skin yoghurt comprises the following raw materials: fresh milk, milk skin, sugar, cream, condensed milk, milk powder, cheese powder, protein powder and carotene. The invention also provides a preparation method of the milk skin yoghurt. The preparation method comprises the following steps: step 1, preparing materials; step 2, cooking: putting the fresh milk into a container, heating the fresh milk in a boiling water bath, and then cooling the fresh milk; step 3, blending: stirring the cooled fresh milk, sequentially adding the rest ingredients, and then cooling; step 4, fermentation: putting the mixture into a fermentation machine for fermentation for more than 8 hours; and step 5, inspecting and packaging. According to the milk skin yoghourt food processed by the method disclosed by the invention, through coordination and complementation of probiotics and various nutritional ingredients, the nutritional value in milk is maintained, the nutritional and health-care effects can be provided, and the milk skin yoghourt food is delicious in taste, unique in taste and wide in market prospect.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention discloses a method for improving drought tolerance and yield of wheat through combined application of compost and sodium selenite, and belongs to the technical field of agricultural planting. According to the method, wheat is planted after compost is applied to soil, and when drought stress occurs, a sodium selenite solution is sprayed to wheat leaves, so that oxidative stress indexes such as MDA, H2O2 and O2 <-> of wheat can be remarkably reduced, and the activity of antioxidant enzymes such as SOD, POD, CAT and APX can be improved; physiological indexes such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, protein, soluble sugar and proline of wheat leaves are improved, growth and yield related traits of the wheat are improved, and finally the drought resistance and yield of the wheat are improved. The compost is prepared by taking 50%-55% of corn straw, 10%-20% of flue-cured tobacco straw, 20%-25% of rice straw, 10%-15% of livestock manure and 0.1%-1% of bacillus subtilis as raw materials, adding organic wastewater or tap water, mixing, composting and fermenting.

The invention discloses a recombinant pichia pastoris strain with high yield of retinaldehyde as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing dehydrogenase Zwf1, phosphate dehydrogenase Gnd2, NADH kinase Pos5 and two copied beta-carotene-15, 15 '-monooxygenase BCMO in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-B (Propene Polymer-B). The retinaldehyde production performance of the recombinant strain is verified based on cofactor engineering and the copy number of key enzymes, and the retinaldehyde production capacity of the pichia pastoris is further improved. The recombinant pichia pastoris disclosed by the invention is simple in construction method, can better promote the synthesis of retinol, and is beneficial to industrial production as the engineering strain can efficiently synthesize retinol by using methanol as a unique carbon source through amplification fermentation in a 5L fermentation tank.

The present invention belongs to the technical field of microbial fermentation, and discloses a method for co-producing DHA and carotenoids by schizochytrium fermentation, wherein the method uses schizochytrium as a production strain to produce polyunsaturated fatty acids and carotenoids, adds an NADPH oxidase inhibitor to the fermentation medium, and adopts a fermentation method with staged temperature control. The present invention combines staged temperature-changing fermentation with the addition of an NADPH oxidase inhibitor during microbial fermentation, thereby achieving the co-production of DHA and carotenoids during schizochytrium fermentation, thereby obtaining microbial oils with high DHA and carotenoid content.

The utility model relates to a microwave-assisted device for preparing a carotenoid microcapsule preparation. The microwave-assisted device comprises an emulsifying tank, a microwave cover and a forming tower, the emulsifying tank comprises a feeding bin, a stirring device and a heating device; the emulsifying tank is sleeved with the microwave cover, microwave generators are further arranged on the two sides of the inner wall of the microwave cover, one side of each microwave generator is fixed to the microwave cover, and the other side of each microwave generator is fixed to the outer wall of the emulsifying tank; the microwave generating device is matched with the heating device in the emulsifying tank, so that the emulsifying efficiency of the prepared raw materials can be improved in a relatively short time. The bottom of the emulsifying tank is communicated with the forming tower through a heating pipeline, a discharge port is formed below the forming tower, and a stock bin trolley is arranged under the discharge port; the fully emulsified preparation raw materials are atomized and dried by the forming tower, and are gathered into the bin vehicle through the discharge port, so that the integration of mixing and emulsifying of the raw materials and preparation of the microcapsule preparation is realized.

The present disclosure relates to the field of microbial technology and provides a Haematococcus pluvialis KM2-1 and its applications. The present disclosure provides a Haematococcus pluvialis ( Haematococcus lacustris ) KM2-1, which was deposited at the China Center for Type Culture Collection on April 3, 2025, and its deposit number is CCTCC NO: M 2025704; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in enriching carotenoids; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in preparing products rich in carotenoids. The Haematococcus pluvialis KM2-1 provided by the present disclosure has good tolerance to light stress and can promote the accumulation of carotenoids under high-intensity light stress conditions, thereby better enriching carotenoids.

The invention provides a concentrated chicken soup material. The concentrated chicken soup material is prepared from the following components in parts by weight: 0.1-0.3 part of fresh ginger, 6-8 parts of old hens, 5-7 parts of sanhuang chickens, 1.2-1.4 parts of raw chicken oil, 145-155 parts of drinking water, 6.4-6.8 parts of salt, 1.2-1.8 parts of monosodium glutamate, 1.2-1.8 parts of chicken juice, 4-5 parts of chicken essence, 0.6-0.8 part of beta carotene oil and 0.5-0.55 part of refined chicken oil. The invention also provides a preparation method of the concentrated chicken soup material. The concentrated chicken soup material provided by the invention is good in taste and flavor and relatively low in cost.

The invention discloses an anti-aging composition containing PQQ and a preparation method of the anti-aging composition, and belongs to the field of medicines or health food, the anti-aging composition comprises PQQ, an antioxidant, collagen, hyaluronic acid and a plant extract, and the content of PQQ is 0.1-5%; 1%-10% of collagen; 0.05%-2% of hyaluronic acid; the antioxidant comprises the following components in percentage by weight: 0.5%-5% of vitamin C, 0.1%-3% of alpha-tocopherol and 0.05%-2% of beta-carotene; the composition contains PQQ and various antioxidants such as vitamin C, alpha-tocopherol, beta-carotene, coenzyme Q10 and glutathione, and the components have a synergistic effect, so that free radicals can be effectively removed, oxidative damage can be reduced, and cell aging can be delayed; most of the components in the composition are natural substances or are widely applied to the fields of food and health care products, the safety is high, and the quality and safety of the product can be ensured through the steps of strict preparation process control, accurate component dissolving method, pH value adjustment, filtration and the like.

The invention discloses a genetically engineered bacterium and a method for synthesizing astaxanthin by using the genetically engineered bacterium. According to the genetically engineered bacterium, a CrtE gene, a CarRP gene and a CarB gene are expressed in an original strain. The genetically engineered bacterium disclosed by the invention can produce beta-carotene and astaxanthin at high yield, and the activation culture of the genetically engineered bacterium does not need to add a defective culture medium or antibiotics, so that the genetically engineered bacterium is beneficial to industrial research of large-scale fermentation of astaxanthin.

The invention relates to the technical field of gene engineering, in particular to a tomato SlHXK1 gene and application of the tomato SlHXK1 gene to improvement of weak light tolerance of tomato seedlings. The nucleotide sequence of the tomato SlHXK1 gene is as shown in SEQ ID No. 1. Research finds that the overexpression of the SlHXK1 gene can improve the activity of sucrose anabolic enzymes in tomato stems and increase distribution of dry substances to leaves, so that the content of non-structural carbohydrates in tomato leaves under weak light is increased, the content of carotenoids and soluble proteins in the leaves is increased, and the photosynthetic ability of the leaves is enhanced; therefore, the adverse effect of weak light on dry matter accumulation of tomato seedlings is reduced, the accumulation rate of dry matter of leaves after illumination recovery is promoted, and the weak light tolerance of tomato plants is improved. Therefore, the invention provides a new gene and method for improving the weak light tolerance of the tomatoes, and has important significance for guaranteeing high yield, high quality and annual supply of the tomatoes in China.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention discloses a method for increasing the content of carotenoid in cucumbers, a related biological material and application. The invention discloses a method for increasing the content of carotenoid in cucumbers. The content of carotenoid in cucumbers is increased by knocking out CsNCED4 genes. The CsNCED4 gene is as shown in SEQ ID No.1 (Sequence Identifier Number 1). The CsNCED4 gene is knocked out through a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) technology, and the used target sequences are shown as SEQ ID NO.3 and SEQ ID NO.4. The recombinant expression vector comprises a complementary sequence of the target spot sequence in the method. The recombinant microorganism comprises the target sequence in the method or the recombinant expression vector. The invention provides a method for rapidly creating a cucumber material with high carotenoid content, and provides a theoretical basis and a practice mode for improving the nutritional quality of cucumbers.

The invention discloses a stress-resistant growth-promoting stable fertilizer and a preparation method thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer is prepared by taking soybean meal leavening, pomegranate peel residues, eucommia ulmoides leaf residues, a compound microorganism bacterium solution and a microorganism activating agent as raw materials, two high-salt-tolerance growth-promoting microorganism strains are screened to form a functional microorganism, madura anactinomyces activates the enzyme production activity of salt-tolerant bacillus by secreting metabolite, and the biological organic fertilizer is prepared. And organic matters decomposed by the salt-tolerant bacillus reversely provide a growth substrate for the madura anactinomycetes to form a metabolism reciprocal relationship. The fertilizer provided by the invention can effectively regulate and stabilize soil activity and promote crop growth, can effectively improve plant stress resistance related enzyme activity, root activity and carotenoid content in leaves under salt stress, improves resistance of soybean plants to salt stress adversity conditions, and has wide development potential and application value.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.