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680 results about "Carotene" patented technology

The term carotene (also carotin, from the Latin carota, "carrot") is used for many related unsaturated hydrocarbon substances having the formula C₄₀Hₓ, which are synthesized by plants but in general cannot be made by animals (with the exception of some aphids and spider mites which acquired the synthesizing genes from fungi). Carotenes are photosynthetic pigments important for photosynthesis. Carotenes contain no oxygen atoms. They absorb ultraviolet, violet, and blue light and scatter orange or red light, and (in low concentrations) yellow light.

Mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as construction method and application thereof

ActiveCN120310669AFungiTransferasesIsopentenyl pyrophosphateCytochrome P450 reductase
The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.
Owner:NANJING TECH UNIV +1

Plant carotenoid determination method based on hyperspectrum and machine learning

The invention belongs to the technical field of hyperspectral imaging and machine learning, and discloses a plant carotenoid rapid determination method based on hyperspectrum and machine learning. The measuring system comprises a hyperspectral imaging device, a spectrum preprocessing module, a characteristic wavelength extraction module, a machine learning modeling module and a carotenoid content prediction module which are sequentially connected through a data processing unit. According to the method, spectral data of a plant sample is obtained in combination with a hyperspectral imaging technology, key wavelengths are screened through spectrum preprocessing and a feature extraction algorithm, and a carotenoid content prediction model is established by using machine learning models such as random forest regression, partial least squares regression or a gradient elevator. According to the method, rapid and nondestructive detection of the plant carotenoid can be realized without a chemical reagent, the determination precision and efficiency are improved, and the defects of complex operation, long time consumption and strong destructiveness of a traditional detection method are overcome.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Apple anthrax leaf blight identification five-dimensional data fusion method based on inspection robot

The invention discloses an apple anthracnose leaf blight recognition five-dimensional data fusion method based on a patrol robot, relates to the technical field of anthracnose leaf blight recognition, and solves the problems of limited sensing dimension, large illumination interference and inaccurate spatial positioning in the prior art. Comprising the following steps: S1, acquiring surface curvature characteristics of apple leaves, collecting RGB-D images of the leaves, constructing a three-dimensional topological structure of the leaves, and positioning scab space distribution; s2, acquiring spectral reflection information of apple leaves, quantifying biochemical components of chlorophyll a and carotenoid, and constructing a vegetation index feature set sensitive to diseases; s3, apple tree canopy temperature information is collected, a transpiration anomaly detection model is constructed, and multi-modal fusion of thermal infrared and point cloud data is realized; and S4, integrating spatial distribution, spectral reflection and thermal radiation information, constructing a five-dimensional phenotypic characteristic matrix model, and comprehensively analyzing apple phenotypic characteristics. According to the method, the recognition accuracy under the conditions of blade shielding, uneven illumination, blade posture change and environment interference can be remarkably improved.
Owner:SHANDONG ACADEMY OF AGRICULTURAL MACHINERY SCIENCES

Natural mixed feed additive and preparation method thereof

The invention relates to the technical field of feed formulas, and particularly discloses a natural mixed feed additive and a preparation method thereof. The natural mixed feed additive comprises the following raw materials in parts by mass: 4-7 parts of vitamin E, 5-15 parts of beta-carotene, 10-16 parts of soybean oil, 20-35 parts of deionized water, 5-8 parts of modified probiotic microcapsules and 1-5 parts of an emulsifier, wherein the modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics; the preparation method comprises the following steps: heating 10-16 parts of soybean oil to 35-45 DEG C, then adding 4-7 parts of vitamin E and 5-15 parts of beta-carotene, and stirring; adding 1-5 parts of an emulsifier into 20-35 parts of deionized water, heating to 45-55 DEG C, stirring, and then slowly adding into the mixed solution obtained in the step S1; 5-8 parts of modified probiotic microcapsules are added, and stirring is conducted for 8-12 min under the condition of 100-200 r / min. The natural mixed feed additive disclosed by the invention has the advantage that the defect that the feed additive still has defects in the aspect of reducing intestinal diseases of animals can be overcome.
Owner:SHANDONG NEW ELEMENT BIOTECHNOLOGY CO LTD

Application of regulatory factor in promoting production of DHA (docosahexaenoic acid) from schizochytrium limacinum, method for improving production of DHA from schizochytrium limacinum and preparation method of microbial oil

The invention relates to the technical field of microbial fermentation, and discloses application of a regulatory factor in promoting schizochytrium limacinum to produce DHA (docosahexaenoic acid), and the regulatory factor contains beta-carotene and reduced glutathione. In addition, the invention also provides a method for improving DHA production of schizochytrium limacinum and a preparation method of microbial oil. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium, and adding beta-carotene and reduced glutathione into the culture medium in the fermentation process; in the culture medium, the content of the beta-carotene is 1.5 to 2g / L. The beta-carotene and the reduced glutathione can effectively improve the content of grease and DHA prepared by fermentation of schizochytrium limacinum, the content of DHA can reach 63.9%, and the beta-carotene and the reduced glutathione are non-toxic and high in safety.
Owner:ZHIHE BIOTECHNOLOGY (CHANGZHOU) CO LTD +1

Aptamer OUC-Fx11-5T of fucoxanthin and application thereof

The invention discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application of the nucleic acid aptamer OUC-Fx11-5T, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthin in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-5T and the fucoxanthine is determined to be 68.2 nM through an isothermal titer thermal method, the nucleic acid aptamer OUC-Fx11-5T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to the fucoxanthine, and can be used for preparing the fucoxanthine aptamer OUC-Fx11-5T of the fucoxanthine. The method can be used for identification or detection of fucoxanthine.
Owner:OCEAN UNIV OF CHINA

Carotenoid compositions and uses thereof

Provided herein are pharmaceutical compositions comprising carotenoids, including liposomes that encapsulate carotenoids including ionizable carotenoids such as trans-crocetin. The provided compositions have uses in treating diseases, disorders and conditions associated with, but not limited to, infection, endotoxemia, inflammation, sepsis, ischemia, hypoxia, shock, stroke, lung injury, wound healing, traumatic injury, reperfusion injury, cardiovascular disease, kidney disease, liver disease, inflammatory disease, metabolic disease, pulmonary disorders, blood related disorders and hyperproliferative diseases such as cancer. Methods of making, delivering, and using the pharmaceutical compositions are also provided.
Owner:L E A F HLDG GRP

Saccharomyces cerevisiae MBPSc21 and application thereof

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.
Owner:ZHEJIANG OCEAN UNIV +1

Recombinant pichia pastoris strain with high yield of retinaldehyde as well as construction method and application of recombinant pichia pastoris strain

The invention discloses a recombinant pichia pastoris strain with high yield of retinaldehyde as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing dehydrogenase Zwf1, phosphate dehydrogenase Gnd2, NADH kinase Pos5 and two copied beta-carotene-15, 15 '-monooxygenase BCMO in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-B (Propene Polymer-B). The retinaldehyde production performance of the recombinant strain is verified based on cofactor engineering and the copy number of key enzymes, and the retinaldehyde production capacity of the pichia pastoris is further improved. The recombinant pichia pastoris disclosed by the invention is simple in construction method, can better promote the synthesis of retinol, and is beneficial to industrial production as the engineering strain can efficiently synthesize retinol by using methanol as a unique carbon source through amplification fermentation in a 5L fermentation tank.
Owner:NANJING TECH UNIV

Haematococcus pluvialis KM2-1 and its applications

The present disclosure relates to the field of microbial technology and provides a Haematococcus pluvialis KM2-1 and its applications. The present disclosure provides a Haematococcus pluvialis ( Haematococcus lacustris ) KM2-1, which was deposited at the China Center for Type Culture Collection on April 3, 2025, and its deposit number is CCTCC NO: M 2025704; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in enriching carotenoids; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in preparing products rich in carotenoids. The Haematococcus pluvialis KM2-1 provided by the present disclosure has good tolerance to light stress and can promote the accumulation of carotenoids under high-intensity light stress conditions, thereby better enriching carotenoids.
Owner:INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI

Formulations for improving gut health

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.
Owner:DSM IP ASSETS BV

Yarrowia lipolytica engineering bacterium for producing astaxanthin in peroxisome compartment and application of Yarrowia lipolytica engineering bacterium

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.
Owner:NANJING TECH UNIV

Plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning

The invention discloses a plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning, and belongs to the technical field of plant physiological detection. The method comprises the following steps: constructing a hyperspectral plant leaf data set; preprocessing a hyperspectral plant leaf data set; reconstructing the preprocessed one-dimensional spectral vector into a two-dimensional image; taking the generated two-dimensional image as input, constructing a multi-task learning model, and performing training and evaluation; and applying the trained model to a test set to obtain an inversion result of the physical and chemical parameters of the leaf. The method solves the problem of parameter inversion interference caused by the same-spectrum foreign matter phenomenon, achieves the precise modeling of local fine feature extraction and global context relation, solves the inversion precision bottleneck caused by spectral response overlapping among parameters, improves the distinguishing capability of the model for easily confused parameters such as chlorophyll and carotenoid, and improves the accuracy of the model. And finally, high-precision and robust synchronous inversion of the physical and chemical parameters of the plant leaves is realized.
Owner:NORTHEAST FORESTRY UNIV

Intelligent monitoring method and system for growth of plants in scenic spot

The invention relates to the technical field of scenic spot plant growth monitoring, and particularly discloses a scenic spot plant growth intelligent monitoring method and system, and the method comprises the steps: carrying out the multi-band image data collection of ornamental vegetation, and obtaining leaf spectrum purification data; extracting wave band response curves of anthocyanin, carotenoid and chlorophyll according to the spectral purification data to obtain pigment wave band characteristic data; performing spectrum aliasing sensitivity analysis on the pigment wave band characteristic data to obtain spectrum aliasing nonlinear characteristic data; carrying out differentiation attribution calculation on a wave band aliasing region between the healthy purple leaves and the iron-deficient yellowing to obtain aliasing difference attribution data; and finally obtaining vegetation pigment unmixing mapping data. Performing physiological status difference identification on the pigment unmixing mapping data to obtain a vegetation health monitoring result; the precision, high efficiency and foresight of scenic spot vegetation monitoring are technically guaranteed, the technical requirements of modern scenic spot vegetation management are met, and losses caused by vegetation health problems are effectively reduced.
Owner:SUZHOU YUNJING GARDEN TECHNOLOGY CO LTD

Application of riboflavin to inhibition of postharvest yellowing of broccoli

The invention provides application of riboflavin in inhibition of postharvest yellowing of broccoli, discovers that yellowing and aging of broccoli can be effectively inhibited by soaking broccoli with a riboflavin-containing solution, and the mechanism of riboflavin in inhibition of broccoli yellowing is deeply studied. The riboflavin is found to be capable of inhibiting expression of chlorophyll degradation genes and chlorophyll aging genes in the broccoli and delaying synthesis of carotenoids by the broccoli, so that yellowing and aging of the broccoli are effectively inhibited. The broccoli treated by the method disclosed by the invention has an excellent treatment effect, and yellowing and aging of the broccoli can be obviously delayed and the shelf life can be prolonged when the broccoli is stored at normal temperature, so that the method has great significance in storage of the broccoli and is also greatly helpful for production and sales of the broccoli.
Owner:ZHEJIANG WANLI UNIV

Formulations for improving gut health

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.
Owner:DSM IP ASSETS BV

Application of SlBRL2 gene in improvement of tomato fruit quality

The invention discloses application of an SlBRL2 gene in improvement of tomato fruit quality, and belongs to the technical field of plant genetic engineering. According to the invention, the gene SlBRL2 related to the tomato fruit quality is found, and the CDS sequence of the gene SlBRL2 is as shown in SEQ ID NO. 1. It is found for the first time that the knockout of the SlBRL2 gene can significantly improve the tomato fruit shape index, the fruit hardness, the soluble solid content, the total sugar content and the carotenoid content, the taste and the nutritional quality of tomatoes are improved while the storage resistance and the transportation resistance of the tomatoes are improved, and a new direction is provided for tomato germplasm breeding.
Owner:NORTHWEST A & F UNIV

Non-destructive detection method for content of chlorophyll and carotenoid in tobacco leaves

The invention provides a non-destructive detection method for the content of chlorophyll and carotenoid in tobacco leaves, and belongs to the technical field of image data process.The method comprises the steps that a standardized image collection environment is constructed, and RGB images are obtained through an industrial area-array camera; performing threshold segmentation, contour extraction and rotation correction by using an HSV color space to obtain a standardized leaf image; a double-branch CNN network structure is constructed, and three attention mechanisms of channel attention, space attention and cross attention are integrated; training the network through an Adam optimizer, and evaluating the performance of the model by using a decision coefficient, a root-mean-square error and a relative analysis error; and finally, carrying out visual analysis by adopting a Grad-CAM technology, verifying the recognition effect of the model on pigment distribution, and realizing high-precision non-destructive rapid detection from image data to pigment content.
Owner:TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (QINGZHOU TOBACCO RESEARCH INSTITUTE OF CHINA NATIONAL TOBACCO COMPANY)

Green fresh-keeping method for relieving low-temperature cold damage of picked tropical fruits

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.
Owner:HUAZHONG AGRI UNIV

A Pickering emulsion stabilized by WG-DNPs and its application in loading β-carotene

A WG-DNPs-stabilized Pickering emulsion and its application in β-carotene loading are disclosed. The emulsion is prepared by the following method: S1: Gluten protein and dextran form a covalent complex through Maillard reaction. The gluten protein complex is then prepared into a solution, and its pH is adjusted to neutral. The ionic strength of the solution is then adjusted to 15-25 mM using NaCl to obtain an intermediate solution. The intermediate solution is subjected to water bath heat treatment, ice bath cooling, and freeze drying sequentially to obtain WG-DNPs. S2: WG-DNPs are dispersed in distilled water to obtain a WG-DNPs dispersion. The WG-DNPs dispersion is mixed and homogenized with an oil phase to obtain the Pickering emulsion. This invention uses glycosylated gluten protein nanoparticles as a stabilizer for Pickering emulsions, giving the Pickering emulsions good thermal and storage stability. Furthermore, by loading β-carotene onto the Pickering emulsion stabilized by WG-DNPs, the bioavailability and digestive stability of β-carotene can be significantly improved, thus aiding in the human body's intake of β-carotene.
Owner:HENAN UNIVERSITY OF TECHNOLOGY

Preparation method of sodium caseinate-epigallocatechin gallate-iron ion ternary complex, product and application

PendingCN120959390AFood ingredient functionsSodium CaseinateBinary compound
The invention relates to the technical field of compositions of high-molecular compounds, and particularly discloses a preparation method of a sodium caseinate-epigallocatechin gallate-iron ion ternary complex as well as a product and application thereof. The preparation method comprises the following steps: S1, adding EGCG (epigallocatechin gallate) into an SC solution, and stirring in a shading environment to obtain a binary compound; s2, adding a FeCl3. 6H2O solution into the binary compound, and stirring in a shading environment to obtain a ternary compound; wherein in the ternary complex, the mass ratio of SC to EGCG to Fe < 3 + > is (10-14): 1: (2-4). According to the preparation method disclosed by the invention, the stable ternary complex can be prepared, the ternary complex utilizes the metal phenolic network to modify the sodium caseinate, and a high internal phase emulsion formed after the sodium caseinate is loaded with the beta-carotene can show excellent stability and relatively high encapsulation efficiency, so that the bioavailability of the beta-carotene can be remarkably improved.
Owner:ZHEJIANG UNIV OF TECH

Application of transcription factor CaNAC2 in regulation and control of pepper fruit maturity

PendingCN120818553APlant peptidesFermentationBiotechnologyCarotene metabolism
The invention discloses an application of a transcription factor CaNAC2 in regulation and control of pepper fruit maturity. The invention relates to the technical field of plant genetic engineering, and provides any one of the following applications of CaNAC2 protein: regulating and controlling ripening of plant fruits; the carotenoid content in the plant fruits is regulated and controlled; the CaNAC2 protein is a protein of which the amino acid sequence is SEQ ID NO: 1. A target fragment of the CaNAC2 gene is amplified in the pepper, a VIGS vector is constructed, and it is found that inhibition of expression of the CaNAC2 gene can regulate the content of carotenoid in pepper fruits and delay fruit maturity, so that the shelf life of the pepper is prolonged. Therefore, the CaNAC2 gene plays an important role in metabolism of capsicum carotenoid, and has important significance on appearance and market value of fruits.
Owner:CHINA AGRI UNIV

Yeast saccharomyces cerevisiae and application thereof

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.
Owner:CHINA TOBACCO SICHUAN IND CO LTD

Yarrowia lipolytica with high yield of beta-carotene as well as construction method and application of yarrowia lipolytica

PendingCN120944727AFungiTransferasesLycopersenePhytoene synthesis
The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.
Owner:JIANGNAN UNIV

Molecular marker related to carotenoid accumulation of patinopecten yessoensis and application thereof

To develop a molecular marker related to carotenoid accumulation for the Patinopecten yessoensis variety "Haidu Jinbei" and to provide a method for maintaining population quality.SOLUTION: An InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis and an application thereof are disclosed. A base sequence of the InDel molecular marker in carotenoid accumulating Patinopecten yessoensis and a base sequence of the InDel molecular marker in common Patinopecten yessoensis are disclosed. Furthermore, an upstream primer sequence and a downstream primer sequence of a primer pair for amplifying the InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis are disclosed. By detecting the InDel molecular marker, homozygous Patinopecten yessoensis individuals capable of accumulating carotenoids can be conveniently and rapidly screened in a seedling stage and from parents, and effective technical support is provided for breeding of "Haidu Jinbei" and optimization of characters.SELECTED DRAWING: Figure 2
Owner:OCEAN UNIV OF CHINA

Directional fermentation method for manifesting fragrance of cigar tobacco leaves

The invention discloses a directional fermentation method for manifesting fragrance of cigar tobacco leaves, and belongs to the technical field of cigar processing. The directional fermentation method comprises the step of spraying an amino acid solution to the cigar tobacco leaves for fermentation. Experiments prove that the sensory quality of the cigar tobacco leaves treated by the directional fermentation method provided by the invention is obviously improved. Meanwhile, the sweet feeling and the mellow feeling are obviously improved, the irritation, the offensive odor and the protein odor are greatly reduced, and the combustibility is improved. Meanwhile, chemical components in the tobacco leaves are more coordinated, the content of neutral aroma substances is increased, and the main effects are that the potassium-chlorine ratio is obviously increased, the nicotine content is reduced, and the contents of carotenoid degradation products and neophytadiene are obviously increased.
Owner:HENAN AGRICULTURAL UNIVERSITY

Compositions and methods for improving carotenoid production

The present disclosure relates to compositions and methods for producing carotenoids. The present disclosure provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a ferredoxin protein, and methods of making and using said genetically modified cells. The present disclosure also provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a CBP or a genetic modification capable of increasing the expression of a CBP, and methods of making and using said genetically modified cells.
Owner:INSCRIPTA INC

Beta-carotene embedded Pickering emulsion as well as preparation method and application thereof

The invention discloses a Pickering emulsion for embedding beta-carotene as well as a preparation method and application of the Pickering emulsion. The Pickering emulsion is obtained by embedding beta-carotene in an emulsifying agent; the emulsifier is a suspension prepared according to the following method: S1, extracting freeze-dried water extraction residues of pterocarya palustris by using an alkaline solution to obtain a filtrate; s2, adjusting the pH value of the filtrate to 6.8-7.3, and centrifuging to obtain a precipitate; s3, dispersing the precipitate in a phosphate buffer solution, performing hydration and shearing treatment, and then performing ultrasonic treatment. The Pickering emulsion disclosed by the invention has a higher BC retention rate after being stored for a short time under low-temperature, dark and low-oxygen conditions; the Pickering emulsion disclosed by the invention still has very high biological accessibility after being subjected to simulated in-vitro digestion, so that the Pickering emulsion stabilized by suspension liquid has very large potential in the aspect of conveying bioactive substances, and theoretical support is provided for practical application of the Pickering emulsion.
Owner:SHANXI AGRI UNIV

Bottle label (Lutein bilberry beta carotene soft capsules)

ActiveCN309739862Sbeta-CaroteneLutein
1. The product name of the design product: bottle label (bilberry lutein beta-carotene soft capsule). 2. The use of the design product: label for the surface of the product packaging. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The design product is a flat product, omitting the left view, right view, top view, bottom view, and rear view, which is not commonly used.
Owner:SHANXI ZHENDONG PHARMA

DHA microcapsule and preparation method thereof

The invention relates to the technical field of nutritious food, and discloses a DHA microcapsule and a preparation method thereof. The DHA microcapsule contains a core material and a wall material, the core material contains DHA oil, squalene and carotenoid, the carotenoid is selected from at least one of beta-carotene, alpha-carotene, lycopene, xanthophyll and zeaxanthine, and the mass ratio of the DHA oil to the squalene to the carotenoid is 1: (0.1-0.55): (0.04-0.16). The DHA microcapsule has relatively low surface oil and a relatively good embedding effect, and active substances in the DHA microcapsule have a relatively high retention rate.
Owner:ZHIHE BIOTECHNOLOGY (CHANGZHOU) CO LTD +1