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461 results about "Carotene" patented technology

The term carotene (also carotin, from the Latin carota, "carrot") is used for many related unsaturated hydrocarbon substances having the formula C₄₀Hₓ, which are synthesized by plants but in general cannot be made by animals (with the exception of some aphids and spider mites which acquired the synthesizing genes from fungi). Carotenes are photosynthetic pigments important for photosynthesis. Carotenes contain no oxygen atoms. They absorb ultraviolet, violet, and blue light and scatter orange or red light, and (in low concentrations) yellow light.

Formulations for improving gut health

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.
Owner:DSM IP ASSETS BV

Yarrowia lipolytica engineering bacterium for producing astaxanthin in peroxisome compartment and application of Yarrowia lipolytica engineering bacterium

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.
Owner:NANJING TECH UNIV

Plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning

The invention discloses a plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning, and belongs to the technical field of plant physiological detection. The method comprises the following steps: constructing a hyperspectral plant leaf data set; preprocessing a hyperspectral plant leaf data set; reconstructing the preprocessed one-dimensional spectral vector into a two-dimensional image; taking the generated two-dimensional image as input, constructing a multi-task learning model, and performing training and evaluation; and applying the trained model to a test set to obtain an inversion result of the physical and chemical parameters of the leaf. The method solves the problem of parameter inversion interference caused by the same-spectrum foreign matter phenomenon, achieves the precise modeling of local fine feature extraction and global context relation, solves the inversion precision bottleneck caused by spectral response overlapping among parameters, improves the distinguishing capability of the model for easily confused parameters such as chlorophyll and carotenoid, and improves the accuracy of the model. And finally, high-precision and robust synchronous inversion of the physical and chemical parameters of the plant leaves is realized.
Owner:NORTHEAST FORESTRY UNIV

Intelligent monitoring method and system for growth of plants in scenic spot

The invention relates to the technical field of scenic spot plant growth monitoring, and particularly discloses a scenic spot plant growth intelligent monitoring method and system, and the method comprises the steps: carrying out the multi-band image data collection of ornamental vegetation, and obtaining leaf spectrum purification data; extracting wave band response curves of anthocyanin, carotenoid and chlorophyll according to the spectral purification data to obtain pigment wave band characteristic data; performing spectrum aliasing sensitivity analysis on the pigment wave band characteristic data to obtain spectrum aliasing nonlinear characteristic data; carrying out differentiation attribution calculation on a wave band aliasing region between the healthy purple leaves and the iron-deficient yellowing to obtain aliasing difference attribution data; and finally obtaining vegetation pigment unmixing mapping data. Performing physiological status difference identification on the pigment unmixing mapping data to obtain a vegetation health monitoring result; the precision, high efficiency and foresight of scenic spot vegetation monitoring are technically guaranteed, the technical requirements of modern scenic spot vegetation management are met, and losses caused by vegetation health problems are effectively reduced.
Owner:SUZHOU YUNJING GARDEN TECHNOLOGY CO LTD

Formulations for improving gut health

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.
Owner:DSM IP ASSETS BV

Green fresh-keeping method for relieving low-temperature cold damage of picked tropical fruits

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.
Owner:HUAZHONG AGRI UNIV

A Pickering emulsion stabilized by WG-DNPs and its application in loading β-carotene

A WG-DNPs-stabilized Pickering emulsion and its application in β-carotene loading are disclosed. The emulsion is prepared by the following method: S1: Gluten protein and dextran form a covalent complex through Maillard reaction. The gluten protein complex is then prepared into a solution, and its pH is adjusted to neutral. The ionic strength of the solution is then adjusted to 15-25 mM using NaCl to obtain an intermediate solution. The intermediate solution is subjected to water bath heat treatment, ice bath cooling, and freeze drying sequentially to obtain WG-DNPs. S2: WG-DNPs are dispersed in distilled water to obtain a WG-DNPs dispersion. The WG-DNPs dispersion is mixed and homogenized with an oil phase to obtain the Pickering emulsion. This invention uses glycosylated gluten protein nanoparticles as a stabilizer for Pickering emulsions, giving the Pickering emulsions good thermal and storage stability. Furthermore, by loading β-carotene onto the Pickering emulsion stabilized by WG-DNPs, the bioavailability and digestive stability of β-carotene can be significantly improved, thus aiding in the human body's intake of β-carotene.
Owner:HENAN UNIVERSITY OF TECHNOLOGY

Preparation method of sodium caseinate-epigallocatechin gallate-iron ion ternary complex, product and application

PendingCN120959390AFood ingredient functionsSodium CaseinateBinary compound
The invention relates to the technical field of compositions of high-molecular compounds, and particularly discloses a preparation method of a sodium caseinate-epigallocatechin gallate-iron ion ternary complex as well as a product and application thereof. The preparation method comprises the following steps: S1, adding EGCG (epigallocatechin gallate) into an SC solution, and stirring in a shading environment to obtain a binary compound; s2, adding a FeCl3. 6H2O solution into the binary compound, and stirring in a shading environment to obtain a ternary compound; wherein in the ternary complex, the mass ratio of SC to EGCG to Fe < 3 + > is (10-14): 1: (2-4). According to the preparation method disclosed by the invention, the stable ternary complex can be prepared, the ternary complex utilizes the metal phenolic network to modify the sodium caseinate, and a high internal phase emulsion formed after the sodium caseinate is loaded with the beta-carotene can show excellent stability and relatively high encapsulation efficiency, so that the bioavailability of the beta-carotene can be remarkably improved.
Owner:ZHEJIANG UNIV OF TECH

Yeast saccharomyces cerevisiae and application thereof

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.
Owner:CHINA TOBACCO SICHUAN IND CO LTD

Yarrowia lipolytica with high yield of beta-carotene as well as construction method and application of yarrowia lipolytica

PendingCN120944727AFungiTransferasesLycopersenePhytoene synthesis
The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.
Owner:JIANGNAN UNIV

Beta-carotene embedded Pickering emulsion as well as preparation method and application thereof

The invention discloses a Pickering emulsion for embedding beta-carotene as well as a preparation method and application of the Pickering emulsion. The Pickering emulsion is obtained by embedding beta-carotene in an emulsifying agent; the emulsifier is a suspension prepared according to the following method: S1, extracting freeze-dried water extraction residues of pterocarya palustris by using an alkaline solution to obtain a filtrate; s2, adjusting the pH value of the filtrate to 6.8-7.3, and centrifuging to obtain a precipitate; s3, dispersing the precipitate in a phosphate buffer solution, performing hydration and shearing treatment, and then performing ultrasonic treatment. The Pickering emulsion disclosed by the invention has a higher BC retention rate after being stored for a short time under low-temperature, dark and low-oxygen conditions; the Pickering emulsion disclosed by the invention still has very high biological accessibility after being subjected to simulated in-vitro digestion, so that the Pickering emulsion stabilized by suspension liquid has very large potential in the aspect of conveying bioactive substances, and theoretical support is provided for practical application of the Pickering emulsion.
Owner:SHANXI AGRI UNIV

Bottle label (Lutein bilberry beta carotene soft capsules)

ActiveCN309739862Sbeta-CaroteneLutein
1. The product name of the design product: bottle label (bilberry lutein beta-carotene soft capsule). 2. The use of the design product: label for the surface of the product packaging. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The design product is a flat product, omitting the left view, right view, top view, bottom view, and rear view, which is not commonly used.
Owner:SHANXI ZHENDONG PHARMA

Embedding method for stabilizing and slowly releasing beta-carotene

The invention discloses an embedding method for stabilizing and slowly releasing beta-carotene. The embedding method comprises the following steps: selecting soybean oil; the stirring conditions are as follows: 300 r / min and 75 DEG C; preparing a water phase: stirring 80 kg of maltodextrin and 20 kg of cane sugar at 50 DEG C until 200 kg of distilled water is completely dissolved; the homogenizing conditions are as follows: the high-speed mixing and shearing emulsifying machine shears for 5 minutes at the speed of 17000 r / min, and the high-pressure homogenizing machine homogenizes for 2 minutes at the pressure of 20 MPa; the spray drying conditions are as follows: the pressure is 0.5 MPa, the inlet temperature is 170 DEG C, and the flow rate is 10 mL / min. The particle size D of the optimized beta-carotene microcapsule product is 95lt; according to the present invention, the prepared nano-particles have characteristics of uniform particle size, 300 nm, embedding rate of 94.2%, good stability in the 90-day storage period, and significantly improved solubility.
Owner:SHANDONG SCENTS JIANYUAN BIO TECH +1

Processing technology of sweet potato leaf powder with high nutrition retention rate

PendingCN121512150AFood freezingFruit and vegetables preservationChlorophyll degradationOxidative enzyme
The invention provides a sweet potato leaf powder processing technology with a high nutrition retention rate, which comprises the following steps: S1, putting sweet potato leaves into a dynamic fixation roller, and mixing nitrogen and carbon dioxide by adopting a nitrogen and carbon dioxide mixing generator to form nitrogen and carbon dioxide mixed gas, the nitrogen and carbon dioxide mixed gas is heated to 95 DEG C through a heat exchanger and sprayed out, the oxygen concentration in the dynamic fixation roller is maintained to be smaller than or equal to 0.5%, and the chlorophyll degradation rate of the sweet potato leaves is monitored through a near-infrared sensor by inhibiting the activity of polyphenol oxidase of the sweet potato leaves; according to the method, a weak acid environment is formed through CO2 dissolution to inhibit the activity of polyphenol oxidase and reduce the requirement for fixation temperature, active ingredients are reserved through three-area gradient variable-temperature drying and rapid dehydration and freeze-drying sublimation, energy consumption is reduced by 37%, heat stability is improved through polyphenol glycosylation, the bioavailability of beta-carotene is improved to 80% through nano embedding, and therefore nutrient loss is avoided, and the method is suitable for industrial production. The drying efficiency, color and flavor are improved.
Owner:张眉

Feed additive for producing high-nutrition low-cholesterol poultry eggs

The invention relates to a feed additive for producing high-nutrition low-cholesterol eggs. The feed additive comprises natural grains, microalgae and a microalgae extract, and is prepared from the following raw materials in percentage by weight: 20-70% of corn, 5-10% of wheat bran, 5-10% of soybean meal, 5-20% of spirulina powder, 5-20% of chlorella powder and 5-20% of haematococcus pluvialis. The feed additive is added into laying fowl feed according to the proportion of 1-2wt%, the feed additive and the laying fowl feed are uniformly mixed and then fed, low-cholesterol poultry eggs rich in protein, algae source carotenoid, calcium, magnesium, zinc, iron and other nutritional ingredients can be stably obtained after the laying fowl feed is fed for 2 weeks, compared with common poultry eggs, the cholesterol content can be reduced by 26%, and the poultry eggs are fine and smooth in taste, free of fishy smell and good in taste. The nutrition and quality of poultry eggs are remarkably improved, and economic benefits are improved.
Owner:QINGDAO INST OF TECH PHYSICS OF SCI & TECH OF CHINA

Tomato postharvest quality regulation and control light supplementing system based on white light-metabolism key enzyme coupling activation and application method of tomato postharvest quality regulation and control light supplementing system

The invention belongs to the technical field of facility horticulture postharvest treatment, and particularly relates to a tomato postharvest quality regulation and control light supplementing system based on white light-metabolism key enzyme coupling activation and an application method thereof, and the tomato postharvest quality regulation and control light supplementing system comprises an enzyme activity targeting white light module, an enzyme activity feedback module and a fruit bearing regulation and control module. According to the postharvest tomato quality regulation and control light supplementing system based on white light-metabolic key enzyme coupling activation, a white light spectrum segmentation-key enzyme activity dynamic matching coupling mechanism is innovatively designed by combining the regulation and control rule of white light on the activity of key enzymes such as carotenoid synthetase and phenylpropane metabolic enzyme of tomato fruits; through an adjustable broad-spectrum white light source and an enzyme activity feedback module, directional accumulation of beneficial metabolites such as carotenoids and flavonoid compounds and efficient degradation of alkaloids are realized.
Owner:DONGGUAN LEDESTAR OPTOELECTRONICS TECH CO LTD

Device for extracting beta-carotene

The invention relates to the technical field of carotene extraction, and discloses a beta-carotene extraction device which comprises a box body, feeding ports are symmetrically formed in the top of the box body, a crushing treatment mechanism is arranged in the box body, a screen connected with the crushing treatment mechanism is arranged in the box body, a guide plate is obliquely arranged below the screen, and a liquid outlet is formed in the position, on the top of the guide plate, of one side of the box body. A stirring assembly is arranged at the lower part in the box body; the crushing treatment mechanism comprises a fixed seat, the fixed seat is fixedly mounted in the middle of the top of the box body, a motor I is arranged at the top of the fixed seat, a rotating shaft is arranged in the box body, a plurality of crushing cutters are symmetrically arranged on the outer wall of the rotating shaft, and a grinding block is connected to the lower end of the rotating shaft and is in a circular truncated cone shape; the output end of the motor I is connected with a driving assembly for driving the rotating shaft to rotate and lift; through the crushing treatment mechanism, the screen linkage and the stirring assembly, efficient crushing, dynamic separation and sufficient mixing are achieved, and the beta-carotene release and extraction efficiency is improved.
Owner:WUXUE GRAMMER BIOTECHNOLOGY CO LTD

A process for the preparation of beta-carotene

The application discloses a preparation method of beta-carotene, which comprises the following steps: 3-methyl-5-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4-pentadienyl triphenyl phosphonium salt (C15 phosphonium salt) cis-trans isomer and 2,7-dimethyl-2,4,6-octatriene-1,8-dial (C10) are dissolved in a water and a water incompatible solvent system, and Wittig reaction is generated under the action of an alkali. The reaction system can avoid that by-product inorganic salt is wrapped in the product, and the content of inorganic salt impurities in the product is reduced. In addition, the proportion of C15 phosphonium salt cis-trans isomer is controlled, a C15 phosphonium salt hydrolysis side reaction is inhibited, and the reaction yield is improved.
Owner:WANHUA CHEM GRP CO LTD

Dietary therapy height increasing method

The invention discloses a food therapy height increasing method. The food therapy height increasing method comprises the following raw materials: enzymolysis bone calcium, oyster peptide, vitamin C, beta-carotene and hemin chloride. According to the present invention, the components such as the enzymolysis bone calcium, the oyster peptide, the vitamin C, the beta-carotene, the heme chloride and the like, which are beneficial to bone repair and bone growth and development, are extracted so as to meet the bone growth requirements of teenagers and children; the raw materials are processed and prepared into powder, all the components are prepared into the solid beverage in a mixing mode, and the solid beverage can be taken by adding sugar or honey or other flavors into water when being eaten. In conclusion, the solid beverage is suitable for teenagers and children to eat, different from a traditional food therapy mode, various growth promoting requirements are met, cooking is not needed, and the solid beverage can be taken after being mixed with water and is convenient to eat.
Owner:朱麒龙

High-absorption carrot juice processing control method based on multi-stage quality detection

The application discloses a high-absorption carrot juice processing control method based on multi-stage quality detection, and relates to the technical field of food processing. The method comprises the following steps: raw material acceptance and initial physical property detection; low-temperature color protection pretreatment; dynamic pressing and initial filtration; enzyme solubilization treatment to release embedded carotenoids; three-stage high-pressure homogenization to reduce the D[4,3] particle size to below 380 nanometers; instantaneous high-temperature sterilization to promote interface membrane reconstruction; multi-stage quality online detection is implemented at four key nodes; closed-loop parameter feedback adjustment is realized based on detection data through a multivariate regression model; and finally, sterile cold filling and light-proof aging are performed. Through the technical scheme, the application significantly improves the carotenoid retention rate, oxidation stability and in-vitro gastrointestinal simulation absorption rate, the final product absorption rate is stably above 42%, the retention rate is above 92%, and no organic solvent or non-original juice processing means is needed, so the application is suitable for industrialized production of high-value-added pure carrot juice.
Owner:QINGDAO PROMIS BEVERAGE CO LTD

Urinary carotenoid testing method, urine collection kit, and method for estimating serum carotenoid concentration or vegetable intake

To provide a urine inspection method which allows detection of a carotenoid concentration in urine in a simple manner.SOLUTION: Provided is a urine inspection method for detecting a carotenoid concentration in urine, by using a urine specimen to which an alkaline additive agent has been added.SELECTED DRAWING: Figure 1
Owner:FUAN KERU

Preparation method of all-trans beta-carotene

The invention discloses a preparation method of beta-carotene. The preparation method comprises the following steps: dissolving an aqueous solution of 3-methyl-5-(2, 6, 6-trimethyl-1-cyclohexene-1-yl)-2, 4-pentadienyl triphenylphosphine salt (C15 phosphine salt) and 2, 7-dimethyl-2, 4, 6-octtriene-1, 8-dialdehyde (C10) cis-trans isomer in a solvent, carrying out wittig reaction under the action of alkali, and carrying out isomerization to obtain the product. By controlling the proportion of C10 cis-trans isomers of the reaction system, the content of the C25 aldehyde intermediate can be reduced, and the reaction yield and the product quality can be improved.
Owner:WANHUA CHEM GRP CO LTD

Application of lycium barbarum Lb03G03224 gene in catalysis of esterification of free carotenoid

The invention discloses application of a lycium barbarum Lb03G03224 gene in catalysis of esterification of free carotenoids, the nucleotide sequence of the lycium barbarum Lb03G03224 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of encoded protein is as shown in SEQ ID NO: 2. According to the present invention, it is found that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate thereof can be generated when the Lb03G03224 gene is expressed in the engineering escherichia coli for generating beta-carotene, beta-cryptoxanthin and zeaxanthin, such that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate can be produced, the protein coded by the Lb03G03224 has the function of catalyzing esterification reaction of carotenoid containing free hydroxyl and fatty acid acyl donor, and has important application value in in-vitro biosynthesis of esterified carotenoid.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

DCAPS molecular marker for identifying sweet potato pulp color character and application of dCAPS molecular marker

The invention discloses a dCAPS molecular marker for identifying sweet potato pulp color characters and application of the dCAPS molecular marker, and belongs to the technical field of sweet potato breeding. According to the dCAPS molecular marker provided by the invention, on the basis of SNP variation sites, mismatched bases are introduced to form restriction endonuclease restriction enzyme cutting sites, the sweet potato pulp color can be judged by utilizing PCR-restriction enzyme cutting-polyacrylamide gel electrophoretic separation, and then sweet potato varieties with high and low carotenoid contents can be identified according to the sweet potato pulp color. The method has important guiding significance and application value for assisting sweet potato carotenoid content related molecular breeding research.
Owner:CHINA AGRI UNIV

A method for preparing color and flavor enhanced whole-ingredient juice by synergistic fermentation of lactic acid bacteria

PendingCN122320144ABiotechnologyFruit juice
This invention discloses a method for enhancing the color and aroma of whole-component fruit juice through synergistic fermentation with lactic acid bacteria, belonging to the field of food processing technology. The method uses ripe mangoes / pineapples as raw materials. After washing, pitting (peeling), and low-temperature jet milling to retain all components, the juice undergoes compound enzymatic hydrolysis and optimization of its uniformity. A compound strain composed of *Lactobacillus plantarum*, *Lactobacillus fermentum*, and *Lactobacillus helveticus* is then inoculated. The mixture undergoes constant-temperature anaerobic fermentation. The process is optimized using fuzzy mathematical sensory evaluation and response surface methodology, resulting in a sensory score of 91.67 for mango juice and 90.83 for pineapple juice. The retention rate of carotenoids is increased by 25%-30%, the lactic acid content reaches 2.97-26.45 mg / g, and the relative content of esters in volatile aroma components increases to over 35%. Simultaneously, the total sugar content decreases by 3.7%-24.0%. This method solves the problems of dull color, weak aroma, and nutrient loss in traditional whole-component fruit juices. The product combines natural functionality with a unique fermented flavor, making it suitable for industrial production.
Owner:SOUTH SUBTROPICAL CROP RES INST CHINA ACAD OF TROPICAL AGRI SCI

Genetically engineered bacterium for high-yield production of beta-ionone and application of genetically engineered bacterium

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.
Owner:SHANGHAI TOBACCO GROUP CO LTD +1

Lycopene composition and emulsion preparation thereof

The invention relates to a lycopene composition and an emulsion preparation thereof. The lycopene composition is prepared from lutein, beta-carotene, all-trans lycopene, 9-cis lycopene, 11-cis lycopene and 13-cis lycopene. The emulsion preparation also comprises an emulsifier, a co-emulsifier, an antioxidant and water, has good solubility in water, can obtain solutions with chromaticity of L30-35, a35-40 and b25-30, and is concentrated in tone value range and stable in tone coloring.
Owner:CHENGUANG BIOTECH GRP CO LTD

A method of facilitating transport to increase retinyl synthesis

The application discloses a method for promoting transport and improving retinal synthesis, which is obtained by introducing an exogenous ABC transport protein Pdr5 into a host fungus and adding a copy of beta-carotene-15, 15'-monooxygenase BLH; wherein the host fungus is a genetically modified Pichia pastoris strain PP-BCCIV. The application further verifies the retinal production performance of the recombinant strain by screening different surfactants, and further improves the ability of Pichia pastoris to produce retinal. At the same time, all the retinal is extracted into the extracellular, and all the carotenes are left in the intracellular, so as to achieve the co-production of carotenes and retinal. The recombinant Pichia pastoris of the application has a simple construction method, can better promote the synthesis of retinal and other vitamin A, and can make the engineering strain utilize glucose and methanol to efficiently synthesize retinal through a 5 L fermentation tank, which is beneficial to industrial production.
Owner:NANJING TECH UNIV

Seed pumpkin magnetic biochar as well as preparation method and application thereof

The invention discloses seed pumpkin magnetic biochar as well as a preparation method and application thereof, and belongs to the technical field of biochar materials. The method comprises the following steps: removing seeds from seed pumpkins, cleaning, slicing, drying, crushing and sieving to obtain seed pumpkin powder with the particle size of less than 212 microns and seed pumpkin residues 1 with the particle size of more than 212 microns; carrying out ultrasonic extraction on the obtained seed pumpkin powder by adopting a deep eutectic solvent, and carrying out solid-liquid separation to obtain a solid, namely seed pumpkin residues 2; mixing the seed pumpkin residue 1 with the obtained seed pumpkin residue 2, and drying; mixing and stirring the obtained solid with an iron salt solution, standing, carrying out solid-liquid separation, taking a solid phase, and drying to obtain pumpkin residue powder; under a protective atmosphere, pumpkin residue powder is pyrolyzed, washed and dried to obtain the seed pumpkin magnetic biochar (PMB). The PMB and carotenoid in the pumpkin seed oil have relatively strong interaction, and the PMB has relatively good reutilization efficiency and can be effectively applied to adsorption of pigments in crude oil of the pumpkin seed oil.
Owner:JIANGNAN UNIV +1