1780 results about "Antioxidant capacity" patented technology

The invention provides isolated Ackermansiella muciniphila, a composition containing the same and an anti-aging and / or anti-oxidation application of the Ackermansiella muciniphila. The Akkermansia muciniphila has the advantages that the average life and the longest life of the Akkermansia muciniphila in a nematode model can be prolonged, the movement activity, the swallowing function and the reproductive capacity of the Akkermansia muciniphila can be improved, the expression of insulin / IGF-1 pathway genes (daf-16, daf-2 and age-1), the expression of antioxidant genes (gst-4 and sod-3) and the expression of longevity related genes (sir-2.1) of the Akkermansia muciniphila can be up-regulated, and the antioxidant capacity and the heat stress tolerance of the Akkermansia muciniphila can be enhanced. It is shown that the Akkermansia muciniphila provided by the invention can be used for preparing or developing the anti-oxidation and anti-aging products, and the anti-oxidation and anti-aging products can be prepared from the Akkermansia muciniphila. The invention provides an efficient and safe solution for anti-aging and / or anti-oxidation.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid and application of the plant lactobacillus MPs-68. The provided plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid is a marine-derived natural strain, the preservation number is CGMCC No.31976, the plant lactobacillus MPs-68 has a remarkable adsorption effect on microplastics polystyrene and polyethylene, has the function of degrading uric acid, and is high in oxidation resistance, gastric acid resistance, cholate resistance and intestinal colonization capacity, and the plant lactobacillus MPs-68 can be used for adsorbing microplastics and degrading uric acid. The method has important application value in the fields of food or health care products for assisting adsorption of micro-plastics and reduction of uric acid and the like. The invention also provides a microbial agent, a probiotic product, a functional food, a fermentation product, a microecological preparation, a microplastic adsorbent and a microplastic adsorbent compounded with the lactobacillus brevis MPA01 (the preservation number is CGMCC No.31977) containing the phytobacterium plantarum MPs-68 capable of adsorbing the microplastics and degrading the uric acid, and the microplastic adsorbent has the effects of adsorbing the microplastics, degrading the uric acid and the like.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The invention discloses a preparation method and application of monatomic iron-doped carbon quantum dot nano enzyme, and the preparation method comprises the following steps: fully dispersing citric acid, urea and iron salt, and fully reacting in a closed container; the ferric salt is any one of ferric citrate, iron methionine or ferrous lactate. The monatomic iron-doped carbon quantum dot nano-enzyme provided by the invention is good in dispersity, excellent in water solubility and uniform and stable in structure, has efficient antioxidant enzyme activity, can be easily absorbed by plants, can remarkably improve the antioxidant capacity and stress resistance of the plants after being applied, can remove excessively accumulated active oxygen of the plants, and also can effectively inhibit the growth of the plants. The soil heavy metal pollution control agent can control soil heavy metal pollution, also can improve the removal efficiency of antioxidant enzymes in plants, reduce heavy metal absorption and increase the plant yield, has the characteristics of small dosage, low cost, high efficiency, high environmental safety and easy use, and provides a new technology and product choice with practical application value for treatment of soil heavy metal pollution.

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle composition and a preparation method and application thereof as well as cosmetics, the anti-wrinkle composition comprises a flos magnoliae extract and an emblic leafflower fruit extract, and the mass ratio of the flos magnoliae extract to the emblic leafflower fruit extract is (0.1-8): (0.5-10); through the synergistic effect of flos magnoliae and fructus phyllanthi contained in the composition, the composition not only has good elastinase inhibition ability, but also has excellent antioxidant ability, so that the composition has good elastinase inhibition ability and good free radical scavenging ability, and further achieves good anti-wrinkle performance.

The invention provides a porcine oocyte in-vitro maturation culture solution additive and application thereof, and belongs to the field of embryo engineering, and the additive contains taurine. The additive also contains sodium pyruvate, cysteine, glucose, NaHCO3, fetal calf serum, estradiol, pregnant horse serum gonadotropin and human chorionic gonadotropin. When the porcine oocyte in-vitro maturation culture solution added with the additive is used for culturing porcine oocytes in vitro, the ROS content in the porcine oocytes can be remarkably reduced, the oxidation resistance of the porcine oocytes can be improved, and the mitochondrial distribution of the oocytes can be improved, so that the development of the oocytes can be improved; the maturation of porcine oocytes and the development of early embryos are promoted. When the technology is applied to the pig assisted reproduction technology, the success rate and quality of embryo engineering can be improved; the pig oocyte in-vitro maturation culture solution added with the additive can improve the premature embryo division rate in parthenogenetic activation of the pig oocytes.

The invention discloses an ulva lactuca composite wall-breaking enzymolysis fermentation product as well as a preparation method and application thereof, and relates to the field of cosmetics. The preparation method comprises the following steps: soaking ulva powder, and performing physical cell wall breaking treatment; mixed enzymes are added for an enzymolysis reaction, wherein the mixed enzymes comprise lipase, cell wall breaking enzyme, enzyme and protease; adding the enzymolysis product into a culture medium, and inoculating bacterial liquid for fermentation culture to obtain fermentation liquid. According to the application, active ingredients such as macromolecular polysaccharide and protein in the ulva lactuca are extracted and decomposed by adopting physical cell wall breaking, composite enzymolysis and bifidus yeast fermentation technologies, and the prepared fermentation product is rich in various composite micromolecular active ingredients such as oligosaccharide, polypeptide, amino acid, polyphenols and flavonoids, has high free radical scavenging capacity, and can be used for preparing the ulva lactuca beverage. The anti-wrinkle and anti-oxidation composition has good anti-wrinkle and anti-oxidation effects, is beneficial to skin absorption when being applied to cosmetics and has high absorption efficiency, and the prepared cosmetics have excellent anti-wrinkle, anti-oxidation, soothing and repairing effects.

The invention provides a microbial source anti-oxidation pigment feed additive for aquaculture and a preparation method of the microbial source anti-oxidation pigment feed additive, the microbial source anti-oxidation pigment feed additive for aquaculture is prepared by the following steps: carrying out low-temperature plasma physical mutagenesis on talaromyces albuminosus CY-G to generate a mutant strain, namely talaromyces albuminosus CY110, and then carrying out semi-solid state fermentation on the mutant strain, namely talaromyces albuminosus CY110 to produce wine red pigment. The pigment product obtained by the invention is high in yield of haematochrome, orange pigment and yellow pigment, low in citrinin content, stable in property and relatively strong in oxidation resistance, can be used as an aquaculture microorganism source anti-oxidation pigment feed additive, and has a wide application prospect. The preparation method disclosed by the invention is simple to operate, low in equipment requirement, high in production efficiency and suitable for large-scale production.

The invention relates to the technical field of microorganisms, in particular to lactobacillus pentosus JX3 and application of the lactobacillus pentosus JX3. The invention provides lactobacillus pentosus JX3 which is high in acid resistance and salt resistance, capable of efficiently degrading nitrite, resistant to oxidation, high in antibacterial capacity and capable of producing acid and amino acid nitrogen. When the lactobacillus pentosus JX3 with good fermentation performance is applied to fermentation, the content of substances such as total acid and amino acid nitrogen can be increased, the oxidation resistance can be improved, the content of ester and alcohol volatile substances with fresh and fruity flavor in a fermented product can be increased, and the product quality can be improved. The edible safety is improved, the shelf life is prolonged, the nutritional value is improved, and the flavor is improved.

The present invention relates to the technical field of microorganisms. Provided are a probiotic composition and a use thereof in the preparation of a product for preventing and / or treating anti-tumor treatment-related diarrhea and other health conditions. Active ingredients of the probiotic composition comprise Bifidobacterium bifidum, Enterococcus avium, Ligilactobacillus salivarius, Limosilactobacillus fermentum, and Parabacteroides distasonis. The probiotic composition has good safety, auto-aggregation capacity, antioxidant capacity, short-chain fatty acid production capacity, in-vitro cell adhesion capacity, capacity to inhibit multiple pathogenic bacteria, and capacity to treat anti-tumor treatment-related diarrhea, etc.

The invention discloses a supramolecular ellagic acid ionic salt composition, a preparation method thereof and application of the supramolecular ellagic acid ionic salt composition in preparing cosmetics. The supramolecular ellagic acid ionic salt composition is prepared from raw materials including ellagic acid, and the concentration of the ellagic acid accounting for the total volume of the supramolecular ellagic acid ionic salt composition is 0.1% w / v-2% w / v; the concentration of PEG accounting for the total volume of the supramolecular ellagic acid ionic salt composition is 15% w / v-85% w / v, and the average molecular weight of PEG is 200-400; the concentration of the antioxidant accounting for the total volume of the supramolecular ellagic acid ionic salt composition is 0.1% w / v-0. 5% w / v; and the molar weight of the basic ion ligand is 1-6 times that of the ellagic acid. According to the supramolecular ellagic acid ionic salt composition and the preparation method thereof disclosed by the embodiment of the invention, the solubility, permeability and formula stability of ellagic acid can be improved on the basis that the ellagic acid has oxidation resistance.

The invention relates to the technical field of protein and peptide, in particular to cubilose peptide with high antioxidant activity and a preparation method of tablets of the cubilose peptide. Comprising the following steps: preparing modified cubilose zymolyte; performing multiple enzymolysis on the modified cubilose zymolyte; the antioxidant stability of the cubilose peptide is enhanced. The resveratrol is grafted on the surface of cubilose protein through free radical reaction of the resveratrol, so that not only can the antioxidant capacity of the cubilose be enhanced, but also the water solubility of the cubilose can be improved, the subsequent enzyme can be better contacted with the cubilose, the enzymolysis efficiency is improved, and the enzymolysis effect is improved. According to the preparation method, cubilose peptide is added into cubilose, reduction of antioxidant activity of the cubilose peptide due to external oxidation factors and too long enzymolysis time is avoided, then the cubilose is subjected to glycosylation modification through chitosan, the dispersion and emulsification stability of the modified cubilose zymolyte can be improved, and the contact area of subsequent enzymes and the modified cubilose zymolyte is increased; the oxidation resistance and the subsequent enzymolysis efficiency are further enhanced.

The invention relates to antioxidant gel for wound healing as well as a preparation method and application of the antioxidant gel. The antioxidant gel is prepared from the following components in parts by mass: 0.01 to 20 parts of hyaluronic acid, 0.01 to 20 parts of cysteine and 1 to 50 parts of polyethylene glycol carboxyl activated ester. The cysteine modified hyaluronic acid and the polyethylene glycol carboxyl activated ester are cross-linked to form a hydrogel network, the tissue adhesion capacity of the hyaluronic acid gel is remarkably improved, the antioxidant capacity and the anti-inflammatory capacity are further improved, and the hydrogel material is simple in component, good in biocompatibility, stable in cross-linked network and biosafe.

The present invention provides a phycocyanin-collagen composite hydrogel and its preparation method and application. The present invention utilizes transglutaminase to catalyze the combination of phycocyanin and collagen to form a new, more stable collagen; transglutaminase acts between protein molecules, utilizes lysine and glutamic acid in phycocyanin and collagen to transfer acyl groups, and cross-links to form new chemical bonds, thereby enhancing the binding strength of phycocyanin and collagen; the hydrogel prepared by the present invention can be used to prepare medical materials and combined with skin injury sites to solve the problem that traditional dressings are easy to adhere to wounds and easily penetrated by tissue fluid and become infected. In addition to ensuring basic air permeability, it reduces the adhesion of the dressing, increases moisture retention and antioxidant properties, and avoids secondary damage when replacing a new dressing. The hydrogel prepared by the present invention has a certain antioxidant capacity and can be used to prepare products with antioxidant effects.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention relates to the technical field of health-care food, in particular to a composition capable of improving water retention and water locking capacity of skin and a preparation method of the composition. Comprising the following steps: preparing starch salmon nasal cartilage composite nano powder; preparing flavor moisturizing tremella polysaccharide fermentation liquor; and light enzymolysis of the salmon nasal cartilage and compounding of the salmon nasal cartilage and the tremella polysaccharide fermentation liquor. According to the invention, the lactobacillus kefiranofaciens kluyveromyces marxianus subsp. Kefiranofaciens ZW3 bacterial liquid, the kluyveromyces marxianus bacterial liquid and the bacillus subtilis DE111 bacterial liquid which are activated and propagated according to a certain proportion are mixed to prepare the compound bacterial liquid, and then the tremella fuciformis extracting solution is fermented, so that the flavor and taste of the tremella fuciformis extracting solution can be improved, the fermentation effect can be enhanced, and the quality of the tremella fuciformis is improved. Healthy nutritional ingredients such as microbial protein, vitamins and amino acid can be efficiently generated, the antioxidant capacity is enhanced, and the prepared flavored moisturizing tremella polysaccharide fermentation liquor is rich in nutritional ingredients and can improve the antioxidant capacity and the water retention and moisturizing capacity of human skin after being taken into a human body.

The invention provides a Wujin pig seminal plasma protein crude extract and application of the Wujin pig seminal plasma protein crude extract in an anti-freezing agent and an anti-freezing diluent for freeze preservation of Wujin pig seminal fluid. The Wujin pig seminal plasma protein crude extract is derived from Wujin pig seminal plasma, does not contain other exogenous bioactive substances, has a better freezing protection effect on sperms of Wujin pigs and is safer, and the acrosome integrity rate of frozen sperms of the Wujin pigs can be remarkably increased. According to the present invention, with the anti-freezing diluent, the vitality and the movement performance of the cryopreserved Wujin pig sperm can be significantly improved, the active oxygen level is reduced, the mitochondrial membrane potential and the energy metabolism are improved, and the oxidation resistance is enhanced, such that the vitality of the Wujin pig sperm can be easily improved, and the mitochondrial function can be easily maintained; the research provides key technical support for establishment of a Wujin pig semen cryopreservation technology, and has great practical significance for Wujin pig breeding and population propagation.

The invention discloses a lactobacillus rhamnosus strain with antiallergic activity and a preparation method thereof, the strain is lactobacillus rhamnosus Lrn-PG-06, the classification name of the strain is Lactobacillus rhamnosus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.3077, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.3077, and the preservation number is CGMCC No.3077. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus Lrn-PG-06 strain is high in acid resistance, alkali resistance and cholate resistance, high in simulated gastrointestinal fluid resistance and high in oxidation resistance, has the advantage of resisting allergy, and solves the problems that at present, allergic diseases are mainly treated by drugs or are prevented from being in contact with allergens in the environment and food, but due to the fact that the drugs are large in toxic and side effects, the allergens cannot be in contact with the allergens in the environment and food. And allergens causing anaphylaxis are difficult to determine, so that allergic diseases are difficult to treat or prevent.

The invention provides a method for detecting the oxidation resistance of traditional Chinese medicine polysaccharide and application of Fe / NC nano-enzyme, and relates to the technical field of biological detection. The method for detecting the oxidation resistance of the traditional Chinese medicine polysaccharide comprises the following steps: providing Fe / NC nano-enzyme; providing a buffer solution, and adding the Fe / NC nano-enzyme, traditional Chinese medicine polysaccharide, hydrogen peroxide and a chromogenic substrate into the buffer solution to form a first reaction solution; and measuring the absorbance value of the first reaction liquid at a specific wavelength, and detecting the oxidation resistance of the traditional Chinese medicine polysaccharide according to the change of the absorbance value of the first reaction liquid. According to the method for detecting the antioxidant capacity of the traditional Chinese medicine polysaccharide, antioxidant activity evaluation on different traditional Chinese medicine polysaccharides can be realized, so that the application of the nano-enzyme in the traditional Chinese medicine polysaccharide is promoted, and the method has important significance in knowing the pharmacological action of the nano-enzyme and developing novel medicines and functional foods.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention relates to the technical field of breeding feed, and discloses an efficient feed additive based on emblic leafflower residue component optimization and a preparation method thereof. Comprising the following components in parts by weight: 30-60 parts of emblic leafflower residues, 10-20 parts of amino acids, 5-15 parts of a vitamin premix, 3-8 parts of a mineral premix, 5-20 parts of probiotics, 0.6-4 parts of cellulase, 0.45-3 parts of pectinase and 0.75-4 parts of amylase. By adding the emblic leafflower residues, the nutrition of the feed additive is enriched, the emblic leafflower residues are rich in cellulose, polyphenol, flavone and other components, have an antioxidant effect, can help animals to remove free radicals in bodies, enhance the antioxidant capacity of the bodies and improve the immunity, meanwhile, the emblic leafflower residues are wide in source and relatively low in cost, and as a feed additive component, the feed cost is reduced; the resource utilization rate and culture benefits are improved.

The invention provides a composition containing elastin peptide as well as a preparation method and application thereof, and relates to the technical field of food. The composition containing the elastin peptide is prepared from the following components in parts by weight: 5 to 10 parts of fish collagen peptide, 3 to 10 parts of tremella polysaccharide, 0.1 to 0.5 part of elastin peptide, 0.01 to 0.1 part of skipjack elastin peptide, 1 to 2 parts of hydrolyzed II-type collagen, 0.01 to 0.05 part of vitamin C, 1 to 5 parts of pomegranate concentrated powder, 1 to 5 parts of cranberry concentrated powder, 5 to 15 parts of lily powder and 1 to 3 parts of blueberry juice powder. The composition containing the elastin peptide is high in safety, and meanwhile, the effects of improving skin elasticity, improving skin moisture, delaying generation of wrinkles and resisting aging are achieved by remodeling internal skin tissues, increasing skin moisture, improving oxidation resistance and the like.

The invention provides a preparation method of Keemun camellia seed fermented oil, and belongs to the technical field of vegetable oil production. The preparation method comprises the following steps: S1, preparing recombinant saccharomycetes; s2, jointly fermenting the antarctic torulopsis, the recombinant saccharomycetes and the Keemun camellia seed oil; s3, separating an oil phase from a water phase, and taking the oil phase; and S4, dehydrating the oil phase to obtain the Keemun camellia seed fermented oil. Preferably, according to the preparation method of the recombinant saccharomycetes in the step S1, phenylalanine deaminase, cinnamate hydroxylase, 4-coumaric acid CoA ligase, chalcone synthetase, chalcone reductase and peroxidase are expressed in saccharomyces cerevisiae. According to the Keemun camellia seed fermented oil prepared by the method disclosed by the invention, the characteristic components of the Keemun camellia seed oil can be remarkably improved, the antioxidant capacity of the Keemun camellia seed oil is enhanced while the skin feeling of the Keemun camellia seed oil is improved, and the application value of the Keemun camellia seed oil in cosmetics is improved.

The invention relates to the technical field of daily chemicals, in particular to a dark tea extract containing eurotium cristatum composite strains for solid aerobic fermentation. The composite strains comprise eurotium cristatum and saccharomycetes. The dark tea extract provided by the invention is stable in performance and not easy to discolor, contains components such as polysaccharide, amino acid and mineral substances, and can help skin moisturize. The permeability of the components is enhanced in the fermentation process, moisture can be locked on the surface layer of the skin, and the moisture degree of the skin is kept. The skin care product has very strong antioxidant capacity, and can neutralize free radicals, slow down the oxidation process of skin, delay skin aging and reduce wrinkles and fine wrinkles. A certain calming effect can be achieved on the skin which is prone to redness and itching or has slight inflammation.

The invention relates to the field of traditional Chinese medicine compositions, in particular to a composition for dispelling effects of alcohol and protecting liver and application. The composition for dispelling the effects of alcohol and protecting the liver comprises the following raw materials: 1-4 parts of calculus bovis factitius, 1-4 parts of artificial bear gall, 35-55 parts of a pseudo-ginseng extract, 40-60 parts of a herba artemisiae scopariae extract, 50-70 parts of a rhizoma smilacis glabrae extract, 30-50 parts of a polygonum cuspidatum extract, 30-50 parts of a radix paeoniae alba extract, 70-90 parts of a pueraria flower extract, 40-60 parts of a rhizoma polygonati extract and 40-60 parts of a dark malt extract. And 25-35 parts of a fructus amomi extract. The hangover-alleviating and liver-protecting composition has the advantages that the hangover-alleviating and liver-protecting effects are similar to that of a positive medicine Haiwang Jinvessel for relieving rat body mass reduction caused by an acute drunkenness model, the hangover-alleviating rate within 6 hours and the liver-protecting and anti-oxidation effects are similar to that of a positive medicine Haiwang Jinvessel, the body mass improvement effect, the hangover-alleviating rate and the anti-oxidation capability of a body are better, and the hangover-alleviating and liver-protecting composition has good development and application values.

The invention discloses application of bifidobacterium adolescentis CCFM1302 in promoting synthesis of host collagen, and belongs to the technical field of microorganisms and medicines. The metagen of the bifidobacterium adolescentis CCFM1302 disclosed by the invention has a good effect of regulating and controlling synthesis and degradation of collagen, the moisture and elasticity of the skin are improved, the antioxidant capacity of the skin is improved, the inflammation level of an organism is reduced, and the stable state of collagen is maintained. The method specifically comprises the following steps: in vitro promoting the enzyme activity of HSF cell LH1 by a thallus lysate, inhibiting the enzyme activity of MMP-3, increasing the content of COL I and COL III and the enzyme activity of LH1, P4H and LOX from the outside of a fermented supernatant, and inhibiting the enzyme activity of MMP-3; the inactivated thalli are orally taken to increase the collagen content and the enzyme activity of LH1 and inhibit the enzyme activity of MMP-1 and MMP-3; the fermentation supernatant is orally taken to inhibit enzyme activity of MMP-3 and MMP-1 and degradation of collagen, and the content of TGF-beta is increased.

Compared with the prior art, the application of the copper-based nano-enzyme to the dual-mode detection of the antioxidant capacity of the tea polyphenol has the beneficial effects that the provided copper-based nano-enzyme is simple to prepare, and an EGCG (epigallocatechin gallate) dual-mode detection method based on the copper-based nano-enzyme is simple, convenient, efficient and high in sensitivity; the method has important application value in the aspect of detecting the equivalent weight of EGCG in the tea leaves and further evaluating the natural antioxidant capacity of the EGCG.

The invention discloses a manganese / cobalt-based MOFs nano-enzyme with a hollow structure as well as a preparation method and application thereof, and belongs to the technical field of preparation and application of novel nano-materials. According to the invention, ZIF-67 is used as a carrier, potassium permanganate is used as a manganese source, and the Mn / Co-MOF nano-enzyme with a hollow structure and highly dispersed manganese species is prepared through a loading-low temperature pyrolysis strategy. The antioxidant substances in the black tea can inhibit the oxidase-like activity of the hollow-structure manganese / cobalt-based MOFs nano-enzyme, so that the color of the system is changed, and based on the principle, the method for detecting the total oxidation resistance of the tea based on the hollow-structure manganese / cobalt-based MOFs nano-enzyme is established. Meanwhile, the method is used for monitoring the change of the total antioxidant capacity in the black tea fermentation process, a new method for judging the fermentation degree of the black tea is established in combination with data processing methods such as machine learning, and a new means is provided for improving the quality of the black tea and intelligently processing tea leaves.

The invention relates to gastrodia elata fermentation liquor as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The invention provides a preparation method of gastrodia elata fermentation liquor, which comprises the following steps: (1) mixing gastrodia elata, mixed enzyme and water, and performing enzymolysis to obtain gastrodia elata enzymatic hydrolysate; and (2) inoculating zymophyte into the gastrodia elata enzymatic hydrolysate obtained in the step (1), fermenting to obtain fermentation liquor, centrifuging the fermentation liquor, taking supernate, and filtering the supernate to obtain gastrodia elata fermentation liquor. The gastrodia elata fermentation liquor obtained according to the scheme disclosed by the invention contains high concentration of p-hydroxybenzyl alcohol and parishin, so that the capacity of removing DPPH free radicals and OH-free radicals is improved, and the total antioxidant capacity of ABTS is also improved.

The invention belongs to the technical field of functional food, and discloses a multifunctional Chinese yam composite peptide composition, a preparation method and application in the aspect of strengthening the heart, the preparation method of the multifunctional Chinese yam composite peptide composition comprises the following steps: preparing pea protein powder; preparing pea peptide powder A; and preparing the multifunctional Chinese yam composite peptide composition. The high-activity pea peptide powder and the Chinese yam powder are subjected to wet graft copolymerization, polysaccharide in the Chinese yam can effectively neutralize the bitter taste of pea peptide, the hydrolysis rate of the pea peptide is increased to 94.32% under the condition that the pH is 2.0-3.0, meanwhile, the oxidation resistance and the gastric mucosa protection capacity of the pea peptide are remarkably improved, the hygroscopicity is improved, and the water content is 8.79%.