1064 results about "Antioxidant capacity" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The present invention provides a phycocyanin-collagen composite hydrogel and its preparation method and application. The present invention utilizes transglutaminase to catalyze the combination of phycocyanin and collagen to form a new, more stable collagen; transglutaminase acts between protein molecules, utilizes lysine and glutamic acid in phycocyanin and collagen to transfer acyl groups, and cross-links to form new chemical bonds, thereby enhancing the binding strength of phycocyanin and collagen; the hydrogel prepared by the present invention can be used to prepare medical materials and combined with skin injury sites to solve the problem that traditional dressings are easy to adhere to wounds and easily penetrated by tissue fluid and become infected. In addition to ensuring basic air permeability, it reduces the adhesion of the dressing, increases moisture retention and antioxidant properties, and avoids secondary damage when replacing a new dressing. The hydrogel prepared by the present invention has a certain antioxidant capacity and can be used to prepare products with antioxidant effects.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention discloses a lactobacillus rhamnosus strain with antiallergic activity and a preparation method thereof, the strain is lactobacillus rhamnosus Lrn-PG-06, the classification name of the strain is Lactobacillus rhamnosus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.3077, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.3077, and the preservation number is CGMCC No.3077. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus Lrn-PG-06 strain is high in acid resistance, alkali resistance and cholate resistance, high in simulated gastrointestinal fluid resistance and high in oxidation resistance, has the advantage of resisting allergy, and solves the problems that at present, allergic diseases are mainly treated by drugs or are prevented from being in contact with allergens in the environment and food, but due to the fact that the drugs are large in toxic and side effects, the allergens cannot be in contact with the allergens in the environment and food. And allergens causing anaphylaxis are difficult to determine, so that allergic diseases are difficult to treat or prevent.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention discloses application of bifidobacterium adolescentis CCFM1302 in promoting synthesis of host collagen, and belongs to the technical field of microorganisms and medicines. The metagen of the bifidobacterium adolescentis CCFM1302 disclosed by the invention has a good effect of regulating and controlling synthesis and degradation of collagen, the moisture and elasticity of the skin are improved, the antioxidant capacity of the skin is improved, the inflammation level of an organism is reduced, and the stable state of collagen is maintained. The method specifically comprises the following steps: in vitro promoting the enzyme activity of HSF cell LH1 by a thallus lysate, inhibiting the enzyme activity of MMP-3, increasing the content of COL I and COL III and the enzyme activity of LH1, P4H and LOX from the outside of a fermented supernatant, and inhibiting the enzyme activity of MMP-3; the inactivated thalli are orally taken to increase the collagen content and the enzyme activity of LH1 and inhibit the enzyme activity of MMP-1 and MMP-3; the fermentation supernatant is orally taken to inhibit enzyme activity of MMP-3 and MMP-1 and degradation of collagen, and the content of TGF-beta is increased.

The invention discloses a Pediococcus pentosaceus strain and an application thereof. Belongs to the technical field of functional component conversion. According to the content of total saponin and polysaccharide in American ginseng, a lactic acid bacterium capable of efficiently converting saponin and polysaccharide is screened by taking a comprehensive score as an index, is identified as pediococcus pentosaceus through morphology, physiology, biochemistry and molecular biology and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33430. The contents of total saponins in the transformed American ginseng and ginseng are respectively increased by 53.90% and 209.61%, and the contents of polysaccharides are respectively increased by 124.68% and 68.16%. The DPPH antioxidant capacity, the ABTS antioxidant capacity, the blood sugar reducing capacity and the blood fat reducing capacity of the converted American ginseng and ginseng total saponin extracting solution and the polysaccharide extracting solution are all improved. The pediococcus pentosaceus obtained by screening has a wide application prospect.

The invention belongs to the technical field of biological medicines, and particularly discloses a composition with functions of reducing blood sugar and blood fat as well as a preparation method and application of the composition. Chinese herbal compound medicinal materials are subjected to water extraction and high-temperature and high-pressure sterilization, and lactobacillus rhamnosus SHA113 bacterial sludge is resuspended in a water extract to obtain the composition of lactobacillus rhamnosus SHA113 and the Chinese herbal compound; the traditional Chinese medicine compound composition comprises astragalus membranaceus, raw Chinese yam, hawthorn, gynostemma pentaphylla, liquorice and jujube; the preservation number of the lactobacillus rhamnosus SHA113 is CCTCC (China Center For Type Culture Collection) NO: M 2017838; the lactobacillus rhamnosus SHA113 and traditional Chinese medicine compound composition can remarkably reduce the content of triglyceride and cholesterol in serum, control the fasting blood-glucose level, relieve accumulation of triglyceride and cholesterol in the liver and improve the oxidation resistance of the liver, has wide market potential and application prospects, and has wide application prospects. The compound can be applied to preparation of drugs for reducing blood sugar and blood fat.

The invention relates to the technical field of genetic engineering and biological agents, in particular to a hybrid peptide with immunoregulation and anti-oxidation functions and a preparation method and application of the hybrid peptide. The polypeptide provided by the invention has immunoregulation and antioxidation functions at the same time, and in a normal or immunosuppression state, the polypeptide can significantly improve the immunologic function of a body and reduce the damage of immunosuppression to the body; the oxidation resistance of cells and organisms can be enhanced; meanwhile, the polypeptide has the advantages of low cytotoxicity, high safety, simplicity and convenience in preparation and low cost, can be used as an ideal immunomodulator and an antioxidant, is widely applied to the fields of medicines, foods, health care, feeds, nutrition and the like of human and animals, and has very good application potential and value.

The invention relates to the field of animal feed science and processing, and discloses an efficient fattening type compound feed for meat gamegalors and a preparation method thereof.The preparation method includes the steps that firstly, specific compound plant raw materials and screened compound dominant flora are adopted for multi-step solid-state symbiotic fermentation, and a fermentation base material rich in probiotics, small molecule peptides and low anti-nutritional factors is prepared; secondly, core functional components including high-activity probiotics, plant extracts, functional lipids, antioxidants and the like are subjected to targeted sustained-release microcapsule coating through a multi-layer composite material; and finally, mixing the fermentation base material with targeted slow-release microcapsules and other conventional feed components, and preparing the compound feed by adopting a low-temperature granulation process. The nutritional value and the utilization rate of the feed can be remarkably improved, and functional components are effectively protected and delivered in a targeted manner, so that the growth and development of the meat gamecocks are promoted, the immune function and the oxidation resistance of the meat gamecocks are enhanced, the meat quality is improved, and the overall health level is improved.

It relates to Centella asiatica-derived exosomes, which is useful in the treatment and / or care of skin. In particular, Centella asiatica-derived exosomes is effective in enhancing skin whitening, reducing skin wrinkles, enhancing skin hydration, and improving skin antioxidant capacity.

The invention provides zero-fat low-sugar yoghourt and a preparation method thereof, the zero-fat low-sugar yoghourt comprises the following raw materials: skimmed milk, a fermentation inoculant, a sweetening agent and a protein product, the fat content of the skimmed milk is less than or equal to 0.3 g / 100mL, the protein content of the skimmed milk is greater than or equal to 6.0 g / 100mL, and the lactose content of the skimmed milk is less than or equal to 4.0 g / 100mL; the fermentation inoculant is composed of pichia kudriavzevii, lactobacillus helveticus and streptococcus thermophilus, and the ratio of the three strains is 2: 1: 5. According to the zero-fat low-sugar yoghourt disclosed by the invention, through selection of specific strains, good stability can be realized under the condition that a thickening agent and a stabilizing agent are not added, the product can be endowed with better oxidation resistance, human body metabolism improving efficacy and immunity regulating efficacy, and a green, clean and healthy yoghourt product is really obtained.

The invention relates to a varied massinia, and in particular relates to a separated varied massinia P2-4 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the varied massinia P2-4 is used for phagocytosis in a bacterium cracking manner, so that the activity of the varied massinia P2-4 is improved. The compound has phagocytosis activity on pathogenic bacteria such as pseudomonas aeruginosa, shewanella alginosa, aeromonas cainea, aeromonas hydrophila and photobacterium damsela; the Marsinia variabilis P2-4 provided by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; by adding the varied maleinia sp. P2-4 or the microbial inoculum thereof into the aquaculture feed, the immunity and / or the oxidation resistance and / or the disease infection resistance of the organism of aquaculture animals can be enhanced; the varied marssonia sp. P2-4 disclosed by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The invention discloses a metagen prepared from bifidobacterium adolescentis and used for promoting synthesis of host collagen, and belongs to the technical field of microorganisms and medicines. The metagen of the bifidobacterium adolescentis CCFM1386 provided by the invention has a good effect of promoting synthesis of host collagen, improves moisture and elasticity of skin, and meanwhile, can improve the antioxidant capacity of the skin and maintain the stable state of collagen. The method specifically comprises the following steps: promoting the enzyme activity of the HSF cell LH1 in vitro by a thallus lysate, and improving the content of COL I and COL III and the enzyme activity of the LH1 in vitro from a fermented supernatant; the bifidobacterium adolescentis has the advantages that the content of collagen is increased by orally taking inactivated thalli, the enzyme activity of LH1, P4H and LOX can be improved by orally taking self-fermentation supernate, the synthesis of the collagen is promoted, and the content of the collagen is increased, so that the bifidobacterium adolescentis has huge application prospects in preparation of oral medicines for promoting the synthesis of host collagen and preparation of foods, health-care products or cosmetics.

The invention belongs to the technical field of microbial fermentation, and discloses lactobacillus mucilaginosus and application thereof in preparation of a uric acid reducing preparation. The survival rate of the fermented lactobacillus mucus in simulated gastric juice with the pH value of 2.0 for 3 hours is greater than 92%, the survival rate in simulated intestinal juice environment containing 0.5% of cholate is greater than 86%, and the viability of the fermented lactobacillus mucus is obviously higher than that of a conventional strain; and the tea has the effects of reducing uric acid, resisting oxidation and inhibiting pathogenic bacteria. The xanthine oxidase inhibition rate of the natural medicinal and edible compound potion obtained by mixing and co-fermenting the strain and existing bacterial powder is greater than 77% (45% or more higher than that of an unfermented group), and the oxidation resistance after fermentation can be improved by 50% at the same time. The beverage is suitable for being drunk as a daily functional food for a long time, and provides a natural and healthy collaborative diet relieving scheme for patients with hyperuricemia and gout.

The invention discloses a high-load starch-based steady-state morinda citrifolia enzyme spray-dried microcapsule and a preparation method thereof, belongs to the technical field of food spray drying, and provides the high-load starch-based steady-state morinda citrifolia enzyme spray-dried microcapsule which comprises morinda citrifolia enzyme and modified starch N-zorbit M / porous starch. The preparation method comprises the following steps: stirring and mixing the morinda citrifolia enzyme and the wall material according to different proportions to obtain a morinda citrifolia enzyme and wall material mixed solution; and carrying out spray drying on the morinda citrifolia enzyme mixed solution to obtain the high-load starch-based morinda citrifolia enzyme spray-dried microcapsule. Modified starch N-zorbit M / porous starch is used as a wall material to prepare the high-load morinda citrifolia enzyme spray-dried microcapsule, so that the volatile unpleasant odor of morinda citrifolia enzyme is reduced, and the total phenol content and the oxidation resistance of the morinda citrifolia enzyme are improved.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention relates to a composition for treating large yellow croaker pyramidoniasis as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition comprises the following components: curcumin, bile acid, beta glucan, bacillus subtilis freeze-dried powder, marine red yeast freeze-dried powder, vitamin C, vitamin E and ethylicin. When the feed additive is added into basal feed to feed large yellow croakers infected with the trypanosomiasis, the death rate of sick fishes can be effectively reduced, the polypide loading capacity is remarkably reduced, and pathological injuries of livers and spleens of the large yellow croakers are relieved. In addition, the composition can also improve the non-specific immunity of the fish body, improve the liver function, enhance the antioxidant capacity of the liver, and improve the lipase activity of the intestinal tract at the same time. Therefore, the composition can be used as a medicine or a feed additive for treating the large yellow croaker pyramidoniasis, has the advantages of small dosage and remarkable effect, and provides a new technical approach for solving the problem that the current large yellow croaker pyramidoniasis lacks an effective prevention and control means.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention discloses a plant lactobacillus with light aging resistance and an application thereof. The preservation number of the plant lactobacillus cj001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35419. The fermentation supernatant of the plant lactobacillus cj001 shows good anti-aging activity and in-vitro antioxidant activity, and the growth ability of HaCaT cells is promoted. After the combined fermentation product of the plant lactobacillus cj001 and the lactobacillus paracasei Grx701 is subjected to ultraviolet radiation, the proliferation of HaCaT cells can be promoted, the endogenous antioxidant capacity of human skin cells can be enhanced, the photooxidation damage can be effectively relieved, and the combined fermentation product has a wide application value in the field of preparation of products with antioxidant, anti-aging, anti-ultraviolet damage, anti-photoaging and the like.

The invention discloses lactobacillus fermented concentrated asparagus extracted juice with the effect of preventing helicobacter pylori infection as well as preparation and application of the lactobacillus fermented concentrated asparagus extracted juice, and belongs to the technical field of food biotechnology and microbial fermentation. The concentrated asparagus beverage is prepared from the following raw materials: 20-50 parts of concentrated asparagus extract juice, 50-80 parts of purified water, 2-6 parts of glucose, 2-4 parts of white granulated sugar, 0.01-0.1 part of sodium D-isoascorbate and 0.001-0.01 part of compound lactic acid bacteria. The fermented and concentrated asparagus extraction juice is prepared by taking asparagus as a raw material, crushing, extracting, concentrating and fermenting, and the antioxidant capacity, the inhibition capacity on helicobacter pylori, the prevention and treatment effects on helicobacter pylori infection and the like of the obtained fermented and concentrated asparagus extraction juice are obviously improved. The asparagus extracted juice can be widely applied to the field of prevention and / or treatment of helicobacter pylori infection, and is particularly applied to preparation of drugs for prevention and / or treatment of helicobacter pylori infection.

The invention discloses a method for inhibiting browning of apple juice through combination of ultrahigh pressure and medicinal and edible substances, and belongs to the technical field of fruit processing. In order to solve the problems of color deterioration, nutrition loss and flavor loss caused by enzymatic browning in the processing and storage processes of non-concentrated and reduced apple juice, the technical scheme is characterized in that fresh apples, gastrodia elata and phyllanthus emblica are respectively prepared into juice, gastrodia elata juice and phyllanthus emblica juice are added into the apple juice according to a ratio, and the juice is added into the apple juice according to a ratio. The raw materials are stirred, mixed, packaged and packaged, then subjected to ultrahigh pressure treatment with specific parameters at a constant temperature, and finally subjected to low-temperature storage. The preparation method can effectively inhibit the activity of polyphenol oxidase and peroxidase, delay the browning process and remarkably improve the oxidation resistance of fruit juice, and is mainly used for quality maintenance and browning inhibition of natural and additive-free apple juice and other fruit and vegetable juice.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to the field of microorganisms, and in particular relates to a Brucella intermedia 33, a vegetable preservative and application of the Brucella intermedia 33 and the vegetable preservative. The invention provides a strain of Buttiauxella agrestis 33, and the preservation number of the Buttiauxella agrestis 33 is CGMCC (China General Microbiological Culture Collection Center) No.34251. The invention also provides a preparation method of the Buttiauxella agrestis 33. After broccoli is treated by the strain, the postharvest yellowing of the broccoli can be obviously inhibited. Afterwards, the expression of genes related to chlorophyll metabolism pathways and antioxidant enzymes in the broccoli treated by utilizing the Brucella intermedia 33 is detected through real-time fluorescent quantitative reverse transcription PCR, and the result shows that the Brucella intermedia 33 inhibits yellowing of the broccoli after being picked by activating chlorophyll synthesis and the expression of the genes related to the antioxidant enzymes; and the oxidation resistance of the broccoli is improved.

The invention provides an application of linoleic acid in preparation of a feed for relieving fish salinity stress. According to the application of the linoleic acid in preparation of the feed for relieving the salinity stress of the fishes, related experiments verify that the addition of the linoleic acid enhances the overall tolerance of the fishes to the salinity stress, and under the stress environment that the salinity continuously rises, the stress resistance of the fishes to the salinity stress is improved. Fish has better growth performance, lower physiological stress level, stronger antioxidant ability and immune response and healthier tissue structure, and is beneficial to increase of culture benefits of freshwater fish and marine fish.

The invention relates to the technical field of biological medicine, and discloses bifidobacterium adolescentis YF-01 and application thereof in fermented milk. The bifidobacterium adolescentis YF-01 is separated from adult excrement and is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251308. Experiments prove that the bifidobacterium adolescentis YF-01 has good gastrointestinal fluid tolerance, the survival rate of the bifidobacterium adolescentis YF-01 can reach 90.1% after simulated gastric fluid treatment for 3 h, and the survival rate of the bifidobacterium adolescentis YF-01 can reach 72% after simulated intestinal fluid treatment for 4 h The strain has the self-aggregation rate of 88.83% and the hydrophobicity of 79.41%, has remarkable scavenging capacity on DPPH free radicals and hydroxyl free radicals, shows good antioxidant activity and adhesion capacity, and provides a theoretical basis for application of the strain in fermented milk. The strain and metabolites thereof can be used for preparing food, medicines or health care products capable of regulating intestinal flora and enhancing oxidation resistance, and have remarkable application potential in development of functional fermented milk products.

The invention provides application of a siderophore-producing strain in preparation of selenium-enriched yeast, and belongs to the technical field of microorganisms. According to the invention, firstly, a siderophore-producing strain is separated from soil, the siderophore-producing strain can efficiently produce siderophores, and the yield of the siderophores reaches 43.7 mu g / mL; studies also find that the siderophore produced by the siderophore-producing strain can improve the tolerance of the saccharomyces cerevisiae to sodium selenite so as to prepare the selenium-enriched yeast, and the intracellular selenium content of the prepared selenium-enriched yeast is relatively high and reaches 3.89 mg / g; the antioxidant capacity of the selenium-enriched yeast is also improved compared with that of yeast cultured without selenium, and the selenium-enriched yeast is expected to serve as a safe and effective organic selenium supplement and has good practicability.

The invention provides a bolete concentrated powder composition lipid carrier with an anti-aging effect as well as a preparation method and application of the bolete concentrated powder composition lipid carrier. The lipid carrier is prepared from the following substances in percentage by mass: 10 to 40 percent of bolete extract, 0.2 to 0.8 percent of antioxidant, 45 to 66 percent of solid lipid, 10 to 16 percent of liquid lipid and 4.2 to 7.8 percent of surfactant. According to the bolete concentrated powder composition lipid carrier provided by the invention, liquid lipid with anti-oxidation and light protection effects is mixed into solid lipid to construct a nano carrier matrix with a light protection function. Plant active ingredients with a light protection effect and an anti-oxidation effect and ergothioneine in the bolete extract are jointly loaded in a nano-structure lipid carrier, so that synergistic interaction of multiple effects is realized. The synergistic delivery system of the nanostructured lipid carrier can inhibit the photodegradation reaction of the ergothioneine and the generation of peculiar smell, improve the photostability of the ergothioneine, enhance the oxidation resistance of the ergothioneine in an acid environment, and improve the anti-aging effect.

The invention discloses a preparation method of CsPHT1; the invention discloses modification of selenium element absorbed by tea and application of the gene 3 to feed, and relates to the field of agricultural gene engineering. CsPHT1 (CsPHT1); the gene 3 is GenBank: OP219455.1, gene mutation of D144K and T150V is carried out on the gene 3, and the amino acid sequence after mutation is shown as SEQ ID No: 01; tea tree CsPHT1; 3, the cultivation of D144K and T150V gene mutation seedlings is improved by the gene. The new variety cultivated by the invention can improve the selenium content and the oxidation resistance of the selenized tea polysaccharide without being influenced by the change of pH 5-7; the selenium-supplementing feed is obtained by mixing the high-selenium tea leaves with the feed, and the requirements of the current high-end feed market are met.

The invention discloses a method for increasing the extraction yield of tremella polysaccharide and application, dry tremella is used as a raw material, tremella polysaccharide is effectively extracted by utilizing an ultrasonic-cellulase wall breaking coupling green solvent method, the extraction yield of tremella polysaccharide is effectively increased, the method is green and environment-friendly and is easy to control, and the obtained tremella polysaccharide has excellent biological activity and can be used for preparing the tremella polysaccharide. The water loss rate of water can be reduced, and the moisturizing and anti-wrinkle capabilities are good; the caenorhabditis elegans can effectively remove hydroxyl free radicals, DPPH free radicals and ABTS free radicals, shows strong oxidation resistance, can significantly prolong the survival rate of caenorhabditis elegans under hydrogen peroxide stress, and shows that the caenorhabditis elegans has good oxidative stress resistance.