1086 results about "Bifidobacterium" patented technology

The invention provides a composition of probiotics, a preparation method and application of the composition in promoting sleep, the composition comprises probiotics and active peptide for promoting sleep, the amino acid sequence of the active peptide is shown as SEQ ID NO: 1, and the probiotics are selected from lactobacillus and bifidobacterium. The composition can effectively improve the sleep condition, regulate neurotransmitter secretion, improve the immunity of the organism and maintain the stability of the internal environment.

The invention relates to the technical field of pet health care, in particular to a probiotic composite additive for pet intestinal health and a preparation method of the probiotic composite additive. Comprising the following basic components in percentage by mass: 40% of lactobacillus complex, 10% of bifidobacterium complex, 10% of saccharomyces boulardii, 10% of prebiotic complex, 10% of natural digestive enzyme, 10% of functional plant extract, 10% of carrier and the like. 30% of a bifidobacterium complex group; 15% of saccharomyces boulardii; 8% of a prebiotic compound; 5% of natural digestive enzyme; and 2% of a functional plant extract. The multiple probiotics have a synergistic effect, the effect is better, the prebiotics promote proliferation of the probiotics and can enhance the effect of the additive, meanwhile, through drying treatment, the product is good in stability, long in shelf life and small in package design, the additive can be conveniently added to pet food, intestinal health of pets can be effectively improved, and the preparation method is simple and suitable for large-scale production.

A breast milk-derived Limosilactobacillus fermentum MSJK0025 capable of improving the intestinal flora condition and the use thereof. The breast milk-derived Limosilactobacillus fermentum MSJK0025 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 26426. The breast milk-derived Limosilactobacillus fermentum is a new strain isolated from breast milk of healthy puerperae, and is referred to as Limosilactobacillus fermentum MSJK0025. The strain has a good antibiotic sensitivity and stable inheritance of traits, has a good tolerance to acid, bile salts and artificial gastric / intestinal fluid, and has a fast growth rate and good safety. Furthermore, the strain has a strong intestinal colonization capability and can effectively inhibit intestinal pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Candida albicans, but has no inhibitory effects on intestinal probiotics such as Lactobacillus and Bifidobacterium.

The invention discloses bifidobacterium longum subsp. Longum IMAU12449 and application thereof, belongs to the technical field of microorganisms, and provides the bifidobacterium longum subsp. Longum IMAU12449, the bifidobacterium longum subsp. Longum IMAU12449 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The invention provides application of the bifidobacterium longum subsp. Longum IMAU12449 in preparation of fermented cow milk and / or soybean milk. The bifidobacterium longum subsp. Longum IMAU12449 is used as a potential probiotic to prepare cow milk and soybean milk, and the prepared fermented milk is stable in viable count, moderate in acidity and free of whey precipitation.

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses a livestock composite biological feed prepared from probiotics and a method thereof, and relates to the technical field of livestock feeds. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus niger BNCC147472, enterococcus faecalis BNCC186075, yarrowia lipolytica BNCC193899, nutrient substances, mineral trace elements, vitamins, amino acids and prebiotics. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus The compound biological feed fermented by adopting various probiotics can improve the antioxidant level of the broiler chicken, improve the health condition of intestinal tracts, increase the villus height and the villus-implicit ratio of the jejunum of the broiler chicken and reduce the occurrence of intestinal inflammation, so that the growth performance of the broiler chicken is promoted, and the immune performance of the body is improved.

According to the probiotic agent for enhancing the immunity and improving the intestinal flora and the application of the probiotic agent, five specific different types of bifidobacterium strains are compounded, potential interaction exists among the five strains, and the five strains can cooperate with one another, so that the effect of enhancing the immunity and improving the intestinal flora is achieved. The bifidobacteria has the advantages that the bifidobacteria has synergistic interaction in the aspects of promoting the growth of immune organs and increasing the total quantity and diversity of intestinal flora, the bifidobacteria is matched with basic fermentation strains for yogurt fermentation, meanwhile, the composite prebiotics are added for synergistic yogurt fermentation, the quantity of the bifidobacteria at the end of a shelf life after the yogurt fermentation is finished is still not less than 1 * 10 < 8 > CFU / mL or 1 * 10 < 8 > CFU / g, and the content of the bifidobacteria is not less than the stability of lactic acid bacteria in the yoghourt is remarkably improved, so that the number of viable bacteria which enter the intestinal tract of a human body and play roles is remarkably increased, and the yoghourt has relatively good tolerance in the intestinal tract; the prepared yoghurt is fine and smooth in texture, rich in taste and rich in fragrance; in addition, the immune decline and intestinal flora disorder caused by cyclophosphamide (CTX) can be remarkably improved, and a positive effect on immunological enhancement is achieved.

The invention relates to the field of biological medicine, and provides a preparation method of a fermentation product containing giant salamander peptide, which comprises the steps of stepped enzymolysis pretreatment, staged inoculated fermentation, synergistic enzymolysis and molecular weight grading purification. The method comprises the following steps: firstly, carrying out staged enzymolysis on giant salamander tissues by utilizing pepsin, trypsin and flavourzyme to obtain a primary peptide solution; then, bacillus subtilis, lactobacillus plantarum and bifidobacterium are adopted for directional fermentation, and flora metabolism is optimized through dissolved oxygen control and pH automatic regulation. Then, compound protease is added for secondary enzymolysis, so that the yield of the small molecule peptide is increased. And finally, carrying out ultrafiltration and nanofiltration graded purification, vacuum concentration, freeze drying and gamma-ray sterilization to obtain the micromolecular giant salamander peptide. The molecular weight of the obtained product is mainly distributed within 0.5-5 kDa (lt), and the proportion of 3 kDa is larger than or equal to 80%), the DPPH free radical scavenging rate is larger than or equal to 85%, the ABTS < + > scavenging rate is larger than or equal to 80%, and the obvious anti-oxidation and auxiliary liver protection effects are achieved.

The present invention relates to the technical field of microorganisms. Provided are a probiotic composition and a use thereof in the preparation of a product for preventing and / or treating anti-tumor treatment-related diarrhea and other health conditions. Active ingredients of the probiotic composition comprise Bifidobacterium bifidum, Enterococcus avium, Ligilactobacillus salivarius, Limosilactobacillus fermentum, and Parabacteroides distasonis. The probiotic composition has good safety, auto-aggregation capacity, antioxidant capacity, short-chain fatty acid production capacity, in-vitro cell adhesion capacity, capacity to inhibit multiple pathogenic bacteria, and capacity to treat anti-tumor treatment-related diarrhea, etc.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Longum as well as a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement containing the bifidobacterium longum subsp. Longum and application of the bifidobacterium longum subsp. Longum. The bifidobacterium longum is preserved in the Guangdong Microbial Culture Collection Center on January 21, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.65836. The bifidobacterium longum subsp. Longum has relatively strong gastric acid and cholate tolerance and intestinal colonization capability; the bifidobacterium longum subsp. Longum can effectively utilize bifidobacterium bifidum to release monosaccharide or disaccharide outside cells for efficient proliferation, and the composition containing the bifidobacterium longum subsp. Longum, the bifidobacterium bifidum and breast milk oligosaccharide can release a large amount of short-chain fatty acid, so that the effects of maintaining the physiological function of an organism and improving the health of the organism are achieved.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention belongs to the technical field of microorganisms. The invention relates to a probiotic composition and application thereof in preparation of products for preventing and / or treating health conditions such as anti-tumor treatment related diarrhea. The probiotic composition comprises the following active ingredients: bifidobacterium bifidum, enterococcus faecalis, lactobacillus salivarius, lactobacillus fermentum mucus and parabacteroides didysonii. The probiotic composition has good safety, self-agglutination capability, oxidation resistance, short-chain fatty acid production capability, in-vitro cell adhesion capability, capability of inhibiting a plurality of pathogenic bacteria, capability of treating anti-tumor treatment related diarrhea and the like.

The invention relates to the technical field of biological medicine, and discloses a formula of compound probiotic enzyme powder for promoting gastrointestinal microecological balance and nutrient absorption, by constructing a cross feeding system of bifidobacteria, lactobacillus and butyric acid bacteria and combining a stomach-intestine stage enzyme activity gradient adaptation strategy and a microenvironment response type double-layer embedding technology, the gastrointestinal microecological balance and nutrient absorption can be promoted, and the gastrointestinal microecological balance and nutrient absorption can be promoted. And cross-scale synergy of flora metabolism, substrate conversion and targeted release is realized. Wherein the sodium alginate-pectin composite embedding structure forms a pH buffer micro-area in a gastric acid environment, and proton pump gene expression of the acid-resistant strain is synchronously triggered; the prebiotics compounding and step-by-step enzymolysis process breaks through a closed-loop path of fiber degradation-flora proliferation-short-chain fatty acid generation. Compared with a traditional single-strain intervention scheme, the method has the advantages that double limitations of flora function splitting and process isolation are broken through, and systematic technical advantages are formed in the aspects of improving flora colonization stability, enhancing metabolite conversion efficiency and inhibiting pathogenic bacterium colonization.

The present invention relates to a method of increasing nicotinamide mononucleotide (NMN) and / or nicotinamide adenine dinucleotide (NAD +) in the intestinal tract of a subject, the present invention relates to a method for treating a subject by administering to the subject a combination of at least one Bifidobacterium sp. Capable of growing on a sialylated HMO and having a functional de novo nicotinamide adenine dinucleotide (nad operon (nadC, nadD, and nadE) and at least one fucosylated oligosaccharide.

The invention relates to the technical field of functional health care products, and discloses a cordyceps militaris probiotic beverage for regulating intestinal flora balance, the probiotic beverage is composed of the following materials by weight: cordyceps militaris extract; a probiotic strain; inulin; fructo-oligosaccharide; a stevia rebaudiana extract; honey; vitamin and mineral supplements; and the balance of distilled water. Studies prove that bifidobacterium, lactic acid bacteria and lactobacillus acidophilus have positive benefits in the aspect of regulating intestinal flora, growth of beneficial bacteria can be promoted, harmful bacteria can be inhibited, and honey, vitamins and mineral supplements are beneficial to improving the overall taste and improving the immunity of the human body. More nutrition support can be provided for intestinal microflora, the balance of the intestinal flora can be maintained and repaired, and the beneficial effect of screening strains aiming at specific health benefits and specially adjusting the balance of the intestinal flora is achieved.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The invention discloses a panax quinquefolius probiotic beverage and a preparation method thereof, and relates to the technical field of functional foods. According to the beverage, the combination of the American ginseng extract and specific probiotics is taken as a core, the survival rate of thalli is increased by adopting a microcapsule embedding technology, and the product performance is optimized by adding a stabilizer and a flavor regulator. The formula comprises 5-15% of a panax quinquefolius extract, 3-8% of compound probiotic freeze-dried powder (lactobacillus plantarum, lactobacillus salivarius and bifidobacterium lactis), 10-25% of inulin, 5-15% of whey protein, 10-30% of a microcapsule wall material (sodium alginate / chitosan or a WPI-KGM compound), and auxiliary materials. According to the preparation method, the active ingredients are efficiently reserved through the processes of gradient extraction, microcapsule embedding, low-temperature drying and the like. The product has the functions of resisting fatigue, regulating intestinal flora and enhancing immunity, the survival rate of probiotics in simulated gastric juice reaches 85% or above, and the shelf life stability is improved by 40%.

The invention discloses a synbiotics composition with bacteriostatic and anti-inflammatory effects, which comprises probiotics and prebiotics in parts by weight, the probiotics comprise the following components in parts by weight: 0.05 to 0.15 part of freeze-dried powder of lactobacillus rhamnosus PB-LR76, 0.05 to 0.15 part of freeze-dried powder of lactobacillus acidophilus HH-LA26, 0.05 to 0.15 part of freeze-dried powder of lactobacillus bulgaricus HH-LB57, 0.05 to 0.15 part of freeze-dried powder of bifidobacterium lactis HH-BA68, 0.05 to 0.15 part of freeze-dried powder of lactobacillus reuteri PB-LR09, and 0.05 to 0.15 part of freeze-dried powder of lactobacillus plantarum HH-LP56; the prebiotics are fructo-oligosaccharide or a rhizoma polygonati extract. Compared with the single use of probiotics or prebiotics, the synbiotics composition disclosed by the invention has stronger antibacterial and anti-inflammatory effects. The invention further discloses application of the synbiotics composition in preparation of a medicine for treating enteritis.

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention discloses bifidobacterium adolescentis as well as a culture method and an identification method thereof. Specifically, the invention provides the bifidobacterium adolescentis GSB03505, the preservation number of the bifidobacterium adolescentis GSB03505 is CGMCC (China General Microbiological Culture Collection Center) No.32483, and the invention provides a method for identifying the bifidobacterium adolescentis. The GABA yield of the bifidobacterium adolescentis reaches up to 3200mg / L and is obviously higher than that of the existing strain; in addition, the strain has the potential of being applied to living fungus food, health care products or medicines for improving sleep and regulating or treating mental diseases.

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

A Bifidobacterium longum subsp. infantis YLGB-1496 postbiotic product, a bacteriocin, a preparation method, and a use, specifically relating to the technical field of probiotic postbiotics. The provided probiotic postbiotic product has the following biological functions: enhancing the intestinal barrier function, preventing and / or treating inflammatory bowel diseases, regulating gut flora, relieving intestinal flatulence and resisting oxidation, and has application prospects in preparing health-care foods or drugs for regulating gut flora and resisting oxidation.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention belongs to the technical field of microbial fermentation, and particularly discloses a method for producing gamma-aminobutyric acid by using lactobacillus hilgardii and an application of the gamma-aminobutyric acid. The method comprises the following steps: (1) activating strains; (2) culturing a seed solution; (3) fermentation culture: inoculating the third-stage seed solution into a fermentation tank filled with a fermentation culture medium, and carrying out two-stage fermentation culture to obtain a fermentation solution; in addition, a glucose solution or a sodium glutamate solution is fed in the whole fermentation process, and an agaric water extraction solution and a bifidobacterium longum fermentation solution are fed in the first-stage fermentation and the second-stage fermentation respectively; and (4) inactivating and filtering. According to the method disclosed by the invention, a two-stage fermentation control strategy is adopted in the main fermentation process, and meanwhile, specific supplemented materials are fed in a fed-batch manner, so that the growth of thalli can be effectively promoted, the metabolic state of the thalli is optimized, and more metabolic flux is guided to synthesis of GABA, thereby effectively improving the yield of GABA.

The present invention belongs to the field of probiotic fermentation technology, and specifically relates to a fermented product of Bifidobacterium longum subsp. infantis, and a preparation method thereof, and applications thereof in improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue. The Bifidobacterium longum subsp. infantis NKU FB3-14 composition of the present invention and a probiotic fermented product prepared using the Bifidobacterium longum subsp. infantis NKU FB3-14 composition as a raw material have bioactive substances that are different from the raw materials, thereby achieving the effects of improving testosterone levels and fertilization rates, improving chronic kidney disease, and combating fatigue.

The invention discloses a microbial marker related to refractory diarrhea, a product and application of the microbial marker, and relates to the technical field of biomedicine. According to the present invention, the microbial marker comprises any one or more of Ruminococcus sp, Bifidobacterium longum, Lachnospiraceae bacterium, Klebsiella pneumoniae, Rosa mucilaginosa, and Coprococcus catenus, and the microbial marker comprises any one or more of the following components: the Ruminococcus sp, the Bifidobacterium longum, the Lachnospiraceae bacterium, the Klebsiella pneumoniae, the Rosa mucilaginosa, and the Coprococcus catenus. The microbial marker can serve as a detection marker to be applied to diagnosis of refractory diarrhea patients, and when the refractory diarrhea patients are diagnosed, the microbial marker is completely noninvasive and high in accuracy.