20 results about "Glucoraphanin" patented technology

The invention relates to the technical field of food processing, in particular to a freeze-dried broccoli processing method. The invention provides a freeze-dried broccoli processing method which comprises the following steps: taking cleaned broccoli, and cutting the broccoli into small flower types which are consistent in size, shape, thickness and height; carrying out instantaneous fixation by adopting steam, immediately soaking the broccoli subjected to fixation in ice water, fishing out the broccoli, and draining water; stacking the quick-frozen broccoli and the dry ice layer by layer; and after the temperature of the broccoli is reduced to-40 DEG C to-60 DEG C, performing vacuum freeze drying until the water content is less than or equal to 3% to obtain the dehydrated broccoli. According to the method, the loss of functional components such as glucoraphanin and polyphenol is reduced; formation of ice crystals is reduced, cell damage is reduced to the maximum extent, the content of functional components is effectively maintained, the color is close to that of fresh broccoli, and rehydration performance is good; the retention rates of functional components of glucoraphanin, flavone and vitamin C in broccoli all reach 98% or above.

The invention discloses application of glucoraphanin to preparation of a product for reducing biological accumulation of perfluorooctane sulfonate, and belongs to the technical field of biological medicine. The occurrence probability of the high PFOS level in the body of a participant frequently eating broccoli is confirmed to be remarkably reduced through population epidemiological experiments, and then the PFOS level in the body of the participant is confirmed to be remarkably reduced after glucoraphanin intervention through clinical RCT random control experiments. The glucoraphanin-intervened serum has the advantages that the glucoraphanin-intervened serum is used as a main body, the metabolic change of steroid, ceramide and bile acid in the glucoraphanin-intervened serum is confirmed to be closely related to the reduction of the PFOS level through lipidomics analysis, and finally, the glucoraphanin is confirmed to be capable of effectively reducing the PFOS in-vivo accumulation and improving the liver injury caused by the PFOS through animal experiments and an AI reverse targeting technology; and the glucoraphanin and the in-vivo metabolite sulforaphane thereof have relatively strong binding capacity with a liver-specific PXR receptor, so that a good technical foundation is laid for research and development of drugs for treating diseases related to PFOS accumulation.

The invention discloses a broccoli seed extract glucoraphanin pharmaceutical composition and a preparation method thereof, and belongs to the field of pharmaceutical compositions. The method aims at solving the problems that in a traditional extraction method, the glucoraphanin concentration is low, the extraction efficiency is insufficient, and multiple antioxidant and anti-inflammatory components are difficult to be stably compatible due to physical and chemical differences. The composition contains the glucoraphanin extract with the purity larger than or equal to 92%, quercetin, forsythin and other components, the composition is prepared through the processes of microwave-assisted extraction, high-pressure homogenization, macroporous resin purification and the like, and the purity of all the components reaches the high level. Experiments prove that the composition can strongly inhibit helicobacter pylori, has concentration-dependent antioxidant activity, can resist inflammation, is low in side effect and high in safety, realizes multi-target protection such as oxidation resistance, inflammation resistance and the like through cooperation of multiple components, and is suitable for prevention and treatment of related diseases.

The present invention relates to a nutritional targeting composition which comprises a component 1 providing glucoraphanin, a component 2 providing myrosinase and magnesium stearate and which efficiently releases sulforaphane to activate NRF2 targets. The present invention also relates to the use of the composition in the preparation of products for preventing or treating diseases or conditions which can be prevented or treated by using sulforaphane.

The application discloses application of BoCBL or BoCGS1 in regulating content of glucoraphanin in broccoli and belongs to the field of genetic engineering. The application finds that overexpression of BoCBL or BoCGS1 in broccoli can significantly increase the content of glucoraphanin in broccoli, the content of GRA in the hairy root of the overexpression CBL and CGS1 is increased by 179% and 120% respectively, and the content of the above-ground part is increased by 146% and 30% respectively. Therefore, the two genes can be used for regulating the content of glucoraphanin in broccoli and cultivating a broccoli strain with high glucoraphanin content.

The present application belongs to the field of nutritional composition and biological medicine technology, and particularly relates to a composition for protecting and preserving liver, a preparation method, application and product thereof. The composition of the present application is composed of extract of Silybum marianum, glucoraphanin and extract of Cynara scolymus. The composition of the present application can intervene the pathological process of liver injury from multiple dimensions such as antioxidation, anti-inflammation, regulation of lipid metabolism and repair of hepatocytes through synergistic effect of the three components, significantly reduces the levels of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase, reduces the accumulation of liver fat, improves the antioxidant capacity of the body, effectively prevents or improves alcoholic, chemical, non-alcoholic fatty and autoimmune liver injury, delays the progression of liver fibrosis, and comprehensively maintains the structural integrity and functional stability of the liver.

The present invention relates to a nutritional targeting composition which comprises a component 1 providing glucoraphanin, a component 2 providing myrosinase and magnesium stearate and which efficiently releases sulforaphane to activate NRF2 targets. The present invention also relates to the use of the composition in the preparation of products for preventing or treating diseases or conditions which can be prevented or treated by using sulforaphane.

The present invention discloses a method for preparing broccoli sprout powder rich in glucoraphanin and sulforaphane, belonging to the field of food processing. The broccoli sprout powder preparation method comprises the following steps: screening broccoli seeds, washing, disinfecting, and soaking them, then placing them on a tray lined with filter paper. At an ambient temperature of 25-27°C, the seeds are first germinated with distilled water for 1-2 days, then sprayed with a sucrose solution every 8-12 hours, and allowed to germinate under natural light. The sprouts are harvested after germination for 4-7 days. The harvested sprouts are first steamed, immediately cooled to room temperature, and then soaked in a zinc sulfate solution. After removal and grinding, the pulp is placed in 2 volumes of distilled water and hydrolyzed with endogenous enzymes at 35-40°C. The hydrolyzed broccoli sprouts are freeze-dried and pulverized to obtain the broccoli sprout powder. This broccoli sprout powder is rich in glucoraphanin and sulforaphane and has high nutritional value.

The present disclosure discloses a Lactobacillus plantarum ZUST49 and a bacterial agent, and use thereof, and belongs to the technical field of microorganisms. An aspect of the present disclosure provides a Lactobacillus plantarum ZUST49 and a bacterial agent containing the Lactobacillus plantarum ZUST49, and another aspect provides use of the Lactobacillus plantarum ZUST49 and the bacterial agent containing the Lactobacillus plantarum ZUST49. The Lactobacillus plantarum ZUST49 of the present disclosure may convert glucoraphanin into sulforaphane, especially in a human or animal intestinal environment. After an enteric probiotic preparation prepared from the strain of the present disclosure is orally taken, the Lactobacillus plantarum ZUST49 may efficiently convert the glucoraphanin into the sulforaphane in an intestinal tract, which not only solves a problem that stability of the sulforaphane cannot be improved in the prior art, but also effectively exerts a probiotic effect.

The invention relates to a glucoraphanin and endogenous myrosinase synergistic extraction method and an extraction product, and relates to the technical field of extraction of active ingredients in plants, broccoli seeds and an extraction solution containing an inhibitor are mixed, then high-temperature instantaneous extraction is conducted, an extraction feed liquid is obtained, and the inhibitor contains tea polyphenol and menthol with the mass ratio being (2-3): 1; filtering the extracted material liquid, and performing ultrafiltration on filtrate obtained by filtering to obtain permeate liquid and trapped liquid; the permeate is subjected to instantaneous sterilization treatment, concentration and spray drying, a component containing glucoraphanin is obtained, the trapped fluid is subjected to RO membrane concentration and freeze drying, and a component containing endogenous myrosinase is obtained. According to the extraction method disclosed by the invention, the enzyme and the glucoraphanin can be extracted at the same time under the condition that the enzyme activity is inhibited, and the obtained product is rich in glucoraphanin and also contains myrosinase with certain activity.

The present invention relates to a composition intended to replace TiO2, its manufacturing process and its uses. In particular, the present invention relates to a white-colored composition, having a clarity defined by an L* parameter greater than 85, comprising the mixture of at least two different white-colored compounds, at least one of the two compounds being chosen from calcium carbonate, quartz, zirconia, calcium silicate, mullite, clay, aluminum, starch, bromazolon, cerium oxide, mica, lauroyl lysine, alumina, glucoraphanin, arbidol hydrochloride, arginine, antimony trioxide, tourmaline, nitrile.

Compositions and methods for converting at least one glucosinolate to an isothiocyanate using Bacillus subtilis 839, Bacillus subtilis CO4_4, Pediococcus pentosaceus M3_H01, and / or Pediococcus pentosaceus M2_H12, or active variants thereof, are provided. Conversion of glucosinolates, such as glucoraphanin, to isothiocyanates, such as sulforaphane, leads to the stimulation of the Nrf2 / Keap pathway and phase II enzymes, providing chemoprotective and anti-inflammatory effects. Accordingly, provided herein are compositions comprising Bacillus subtilis 839, Bacillus subtilis CO4_4, Pediococcus pentosaceus M3_H01, and / or Pediococcus pentosaceus M2_H12, or active variants thereof, for administration to subjects for increasing isothiocyanate (e.g., sulforaphane) production, increasing the expression of genes regulated by the Nrf2 transcription factor, including phase II enzymes, decreasing inflammation, and treating or preventing an inflammatory disorder or a cancer. The composition can comprise at least one glucosinolate or a plant, plant part or an extract thereof comprising glucosinolate(s).

The invention relates to a nutrition targeting composition which comprises a component 1 for providing glucoraphanin, a component 2 for providing myrosinase, vitamin C and magnesium stearate and is capable of efficiently releasing sulforaphane and activating an NRF2 target spot. The invention also relates to the use of said composition for the preparation of a product for the prevention or treatment of diseases or conditions that can be prevented or treated with sulforaphane.

The invention discloses a method for increasing the content of beneficial glucosinolates in radishes based on a gene editing technology, and relates to the technical field of radish genetic engineering.The application method comprises the steps that firstly, two target sequences of an RsGRS1 gene are designed, then a promoter and an sgRNA sequence containing two target spots are subjected to gene synthesis and then inserted into a Ubi-CRISPR / Cas9-WPR carrier stored in a laboratory, and the RsGRS1 gene is obtained; and finally obtaining the gene editing vector of the RsGRS1. And transferring the constructed and sequenced plasmid into a host cell, and infecting a rootless seedling explant of the 'NAU-RG' radish by using the plasmid to obtain a transgenic regenerated plant. An rsgrs1 gene mutant is screened from a radish positive transgenic plant, the content of glucosinolate in the mutant plant is further measured, and it is found that the content of beneficial glucosinolate 4-methylsulfinyl butyl glucosinolate (GRA) in the rsgrs1 mutant is increased by 39.66-46.38 times compared with that of a wild type. The invention provides a new method for germplasm creation and genetic improvement of the radish high beneficial thioglycoside component.

A sulforaphane supplement in gummy form, including an active ingredient composition comprising sulforaphane, glucoraphanin, and myrosinase. The supplement including a matrix structure in which sulforaphane, glucoraphanin, and myrosinase are disposed in pores and immobilized therein.

The invention discloses a method for creating a new germplasm of pine flower ball type broccoli with high glucoraphanin content. According to the method, a fertility restoring line pRfo:: Rfo is used as a male parent and hybridized with a broccoli Ogura CMS commercial species of a moss type as a female parent, and the fertility of the broccoli Ogura CMS commercial species is restored; the method comprises the following steps: carrying out marker detection on a Bar transgenic element in an F1-generation plant of a fertility restored broccoli Ogura CMS commercial species to obtain an Rfo-containing F1 hybrid; the method comprises the following steps: hybridizing a high-glucoraphanin fertile parent serving as a female parent and an Rfo-containing F1 hybrid serving as a male parent, and screening to obtain an Rfo-free character excellent material; and hybridizing and screening by taking an Rfo-free material with excellent characters as a female parent and a DMP haploid induction line as a male parent to obtain the new germplasm of the high-glucoraphanin pine flower ball type broccoli. According to the method, the breeding time is greatly shortened, materials with target characters can be quickly and efficiently obtained, and the method has important significance on quickly realizing seed revitalization of domestic varieties.

A skincare composition includes a substance having a predetermined amount of one or more cruciferous vegetables, the predetermined amount of the one or more cruciferous vegetables including stabilized glucoraphanin and active myrosinase in a stable state; exposing the substance to a liquid activator causes a chemical reaction between the stabilized glucoraphanin and the active myrosinase to create sulforaphane in an active product; and applying the activated product to a skin surface allows the sulforaphane to act upon the skin surface.

The invention relates to a skin care preparation containing broccoli seed extract and a preparation method and application, and belongs to the technical field of skin care product preparation, the skin care preparation comprises broccoli seed oil extracted from broccoli seeds, sulforaphane and glucoraphanin, the skin care preparation is a mixed nano-emulsion, the skin care preparation further comprises the following preparation raw materials: Olivem 1000, butanediol and ultrapure water. By selecting and optimizing the extraction solvent and the extraction conditions, the effect of extracting the broccoli seed oil, the sulforaphane and the glucoraphanin from the broccoli seeds at the same time is achieved, the yield of the broccoli seed oil and the yield of the glucoraphanin are increased, and the yield of the broccoli seed oil and the glucoraphanin is increased by compounding the three extracts. The skin care preparation with excellent anti-oxidation, melanin inhibition and anti-inflammatory effects is obtained.

The invention relates to a stable release nutritional targeting composition comprising glucoraphanin, myrosinase and a calcium salt, which can smoothly release sulforaphane. The invention also relates to a method for converting glucoraphanin into sulforaphane in vitro comprising steps of providing the composition and mixing the composition with water or an aqueous solution.

The application provides a method for improving sulforaphane content in fresh-cut broccoli by combining salicylic acid and ultrasonic treatment, and belongs to the technical field of food processing and postharvest preservation. The method unifies moderate fresh-cut injury, salicylic acid induction and ultrasonic cavitation treatment into a continuous process scheme, avoids quality deterioration caused by excessive cutting, promotes accumulation of glucoraphanin and improves contact efficiency of glucoraphanin and endogenous myrosinase, so that the generation level of sulforaphane is improved. The method is simple in steps, short in treatment time, controllable in cost, and suitable for processing of high-sulforaphane fresh-cut broccoli products.