The invention belongs to the technical field of sulforaphene production, and particularly relates to a method for producing high-purity sulforaphene by using glucoraphanin. According to the method, the sulforaphene is prepared by taking glucoraphanin, myrosinase and a dialysis membrane tube as raw materials through the steps of glucoraphanin reaction solution preparation, reaction system preparation, dialysis reaction, reverse osmosis concentration, centrifugal extraction and the like. According to the method, the dialysis membrane tube is introduced for dialysis reaction, so that the in-situseparation of the product from the substrate and enzyme is realized, the degradation rate of the glucoraphanin is significantly improved, the complete retention of the myrosinase is realized, and thedialysis membrane tube can be repeatedly used for the production of the sulforaphene. The method has the characteristics of being simple in production process, stable, easy in organization amplification, high in product yield, low in equipment investment cost, low in production cost, high in comprehensive resource utilization rate and environmentally friendly. The sulforaphene prepared by the method has the characteristics of being high in purity, free of solvent residue and stable in performance, and can be widely used in the industries of medicine, food and daily chemical.