64 results about "Glucoraphanin" patented technology

The invention discloses a method for extracting a glucoraphanin compound from broccoli and cauliflower, which belongs to the technical field of plant components extraction. The method comprises the following steps: (1) selecting and pretreating the plant materials of broccoli and cauliflower; (2) extracting a reagent, performing a crude extraction and concentrating; (3) extracting an organic solvent of the crude extract and removing impurity; (4) carrying out a preliminary purification of the extract; (5) purifying glucoraphanin by chromatographic column and other steps. The invention has the characteristics of simple extracting and purifying method, high extraction yield of glucoraphanin and single components and high purity. The products of the invention can be taken as standard sample for applying to the biochemical experiments and relative researches.

A spray dried myrosinase / ascorbate mixture is formed from the steps comprising: providing a source of myrosinase, adding ascorbate to the source of myrosinase, heating the source of myrosinase to a temperature of about 104° F. (about 40° C.) or higher, and spray drying the myrosinase / ascorbate mixture. The spray dried myrosinase / ascorbate mixture may be used to prepare isothiocyanates. The spray dried myrosinase / ascorbate mixture may also be mixed with glucoraphanin and used in an activated tablet or capsule.

The invention discloses a method for preparing glucoraphanin from broccoli, which comprises the following steps: cleaning the raw material, chopping, adding into an extraction tank set host, and sufficiently contacting solid-phase and liquid-phase substances in the extraction tank set in a reverse motion process, thereby extracting effective components from the medicinal material. The medicine slag are forcedly pushed to a slag outlet to be discharged by a discharge conveyer, and a special squeezing machine extrudes the medicine slag to obtain medicinal material tissues from the residual liquor in the medicine slag, thereby reducing the residual liquor content in the medicine slag. The method has the advantages of higher extraction efficiency and more complete extraction. By adopting the macroporous adsorbent resin combined with silica gel column chromatography for purification, the separating effect is better. The method can be used for preparing the glucoraphanin finished product of which the maximum yield is 1% and the content is higher than 95%.

The invention relates to a method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes, belonging to the technical field of preparation of natural red pigments and proanthocyanidin. The method comprises the following steps: by using red-core radishes as the raw material, extracting, removing proteins, removing glucoraphanin, purifying by ultrafiltration,concentrating, and carrying out spray drying to obtain the radish red pigment homopolymer and radish proanthocyanidin products. The invention has the advantages of great convenience, simple technique, wide raw material sources, and cheap and accessible raw materials; all the materials in the production process can be recycled, so that the material resources are fully utilized and no waste is discharged, and thus, the invention also has the advantages of energy saving and low production cost; and the products have the advantages of high purity, no peculiar smell of radishes, favorable solubility and the like. The invention can be widely used for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes. The products prepared by the method provided by the invention can be widely used in the industries of food, medicines, health products, cosmetics, dyes and the like.

The present invention is directed to chemoprotectant precursor compositions provided from crucifer seeds and sprouts and methods for their preparation. Treatment of aqueous extracts from crucifer seeds or sprouts with adsorbents substantially increases the ratio of certain highly chemoprotectant precursor compounds (alkyl glucosinolates such as glucoraphanin, a.k.a. sulforaphane glucosinolate) to undesirable compounds such as indole glucosinolates (for example 4-hydroxyglucobrassicin). The method provides an extract which has a ratio of glucoraphanin to 4-hydroxyglucobrassicin of about 70 or greater. The resulting extract has improved color and odor and may be dried or used directly as an additive in a variety of foodstuffs.

The invention relates to a glucoraphanin-containing composition which contains a component 1 for providing glucoraphanin, a component 2 for providing myrosinase and a basic salt compound. The composition disclosed by the invention has good stability and overcomes the problems that the content of the glucoraphanin is lowered, a product is discolored and tastes bitter and the like in the prior art.The invention further relates to a pharmaceutical use of the composition.

The invention provides a technique for preparing glucoraphanin-riched broccoli concentrated powder. The technique comprises: selecting and pruning raw material (1), cleaning (2), chopping (3), extracting (4), filtrating (5), beating (6), extracting (7), filtrating (8), concentrating and recovering alcohol (9), drying (10), cooling (11) and then obtaining the product (12); toxic solvents are not contained in the extracting process, making the health-care products safely and reliably; the extracting equipment is simple, do not need improvement on the prior technique, and has advantages of low investment and easy implement.

The invention relates to a method for extracting and separating glucoraphanin from broccoli seeds. The method comprises the following steps that 1, a glucoraphanin solution is extracted from the broccoli seeds and concentrated to obtain a concentrated glucoraphanin solution; 2 the concentrated glucoraphanin solution is adsorbed through macroporous adsorption resin; 3, the macroporous adsorption resin is eluted with an ethanol solution to obtain resin eluate; 4, after the resin eluate is concentrated, silica gel is added, mixing is performed, ethanol is volatilized, and silica gel adsorbing glucoraphanin is obtained and added to the upper layer of a silica gel column obtained through silica gel column filling; 5, the silica gel column is eluted with eluent, eluate is collected, concentrated and dried, and then the glucoraphanin product is obtained. Compared with the prior art, the method has the advantages that the technology is simple and easy to operate, industrialization is easy, the macroporous resin and the silica gel are low in cost and easy to obtain, the whole technology is low in price and little in investment, and the high-purity glucoraphanin product can be obtained.

The invention discloses a fertilizer capable of simultaneously improving contents of glucoraphanin and organic selenium in broccoli. The sulfur content in the fertilizer is 1 to 4 mM, and the seleniumcontent in the fertilizer is 50 to 150 mu M. Meanwhile, the invention further provides a method capable of simultaneously improving contents of glucoraphanin and organic selenium in broccoli. The method comprises the step of foliage spraying of the fertilizer on broccoli plants. By means of the foliar fertilizer disclosed by the invention, the content of glucoraphanin in the broccoli is effectively improved by 27.9 to 89.5%, and the content of organic selenium in the broccoli is improved by 5.1 to 16.5 times.

The invention discloses a moisturizing skin care product. The moisturizing skin care product is prepared from the following raw materials in parts by weight: 14-17 parts of de-ionized water, 8-10 parts of polyvinylpyrrolidone, 1-2 parts of poly dimethyl diallyl ammonium chloride, 6-8 parts of jojoba oil, 10-12 parts of ostrich oil, 12-14 parts of polydimethylsiloxane, 1-2 parts of ethyl maltol, 3-4 parts of glucoraphanin, 4-5 parts of laminine, 3-4 parts of red yeast rice, 2-3 parts of a dateplum persimmon extract, 2-3 parts of a boehmeria siamensis craib herb extract and 2-3 parts of hericium erinaceus polysaccharide. The moisturizing skin care product disclosed by the invention is prepared from various skin-caring nutritional components; and especially, by adding such plant essence ingredients as the laminine, the dateplum persimmon extract, the boehmeria siamensis craib herb extract, the hericium erinaceus polysaccharide and the like, a synergistic effect among various components is enhanced, and the skin care product is quite excellent in effect on moisturizing and improving skin elasticity.

The invention relates to a medicine composition for treating diabetes. A medicine is prepared by adopting glucoraphanin, swertiamarin, hemiphroside B and clerodendrin A as raw material medicines by virtue of proportioning, and the medicine can be prepared into various medicine forms according to a conventional preparation process. By adopting the medicine composition disclosed by the invention, defects of the prior art can be overcome, and the medicine composition for treating diabetes can be provided.

The invention discloses a method for extracting sulforaphane and myrosinase magnetic microspheres, the method comprises the following steps of:(1) heating and stir-frying broccoli seeds, and squeezingoil to remove grease to obtain an oil cake;(2) crushing the oil cake into small particles, soaking and extracting in water to obtain a sulforaphane extract;(3) adding myrosinase magnetic microspheresinto the sulforaphane extract, performing enzymolysis, and recovering the myrosinase magnetic microspheres by magnet adsorption to obtain sulforaphane extract;(4) extracting the sulforaphane by usingdichloromethane to obtain a dichloromethane extract containing the sulforaphane, and concentrating the dichloromethane extract;(5) dissolving the concentrated solution by using acetonitrile, pouringout supernatant, and concentrating to obtain concentrated solution; (6) dissolving the concentrated solution obtained in step (5) by using edible ethanol, pouring out supernatant, and concentrating toobtain the sulforaphane. According to the method, by taking the solidified myrosinase magnetic microspheres as a catalyst, the glucoraphanin is hydrolyzed into sulforaphane, and the purity of the sulforaphane is as high as 95%.

The invention relates to a method for extracting high-purity glucoraphanin. The method comprises the steps as follows: (1) performing enzyme deactivation on broccoli seeds; (2) crushing the broccoli seeds after enzyme deactivation; (3) degreasing the broccoli seeds through petroleum ether extraction; (4) performing aqueous extraction on the degreased broccoli seeds to obtain an aqueous extraction solution; (5) performing concentration, alcohol precipitation and filtration on the aqueous extraction solution to obtain filtrate; (6) performing concentration and spray drying on the filtrate to obtain the high-purity glucoraphanin product. Compared with the prior art, the extraction separation method is simple, adopts a simple and feasible process and is easy to industrialize, the whole process is low in cost and investment, the high-purity glucoraphanin product can be obtained (the purity is 75% or above), and the highest yield is 3.5%.

The present invention relates to the field of food and particularly relates to functional solid drink containing glucoraphanin and a preparation method thereof. The functional solid drink containing the glucoraphanin comprises the ingredients of glucoraphanin, lycium barbarum polysaccharide, a sweetener, fruit powder, inulin and / or konjac refine flour. The glucoraphanin, lycium barbarum polysaccharide and inulin and / or konjac refine flour interact each other, so that the functional solid drink promotes absorption on the one hand, and comprehensively regulates immune system and improves anticancer effects on the other hand.

The invention discloses a method for detecting oxidized form glucoraphanin and reduced form glucoraphanin from a radish extract. The method comprises the following steps: dissolving an oxidized form glucoraphanin and reduced form glucoraphanin standard product into a methanol-aqueous solution, and diluting to obtain a standard product solution with series mass concentration; dissolving the to-be-tested radish extract into a methanol-aqueous solution to obtain a radish extract sample solution; performing high performance liquid chromatography analysis on the standard product solution with series mass concentration and the radish extract sample solution separately to obtain each chromatographic peak area; establishing standard curve equations of the oxidized form glucoraphanin and the reduced form glucoraphanin separately; and substituting the chromatographic peak area of the oxidized form glucoraphanin and the chromatographic peak area of the reduced form glucoraphanin in the sample solution into the corresponding curve equations to obtain the mass concentration of the two types of glucoraphanin in the sample solution, and calculating to obtain the oxidized form glucoraphanin content and the reduced form glucoraphanin content of the radish extract. The method realizes synchronous and quantitative detection of the oxidized form glucoraphanin and the reduced form glucoraphanin, and has the advantages of high speed, high efficiency and high accuracy.

The invention discloses a glucoraphanin composition and application thereof. The invention provides a composition comprising glucoraphanin and a functional oligosaccharide. The invention also relatesto a use of the composition in preparation of a product for chemoprophylaxis and / or treatment of cancer.

The invention belongs to the technical field of sulforaphene production, and particularly relates to a method for producing high-purity sulforaphene by using glucoraphanin. According to the method, the sulforaphene is prepared by taking glucoraphanin, myrosinase and a dialysis membrane tube as raw materials through the steps of glucoraphanin reaction solution preparation, reaction system preparation, dialysis reaction, reverse osmosis concentration, centrifugal extraction and the like. According to the method, the dialysis membrane tube is introduced for dialysis reaction, so that the in-situseparation of the product from the substrate and enzyme is realized, the degradation rate of the glucoraphanin is significantly improved, the complete retention of the myrosinase is realized, and thedialysis membrane tube can be repeatedly used for the production of the sulforaphene. The method has the characteristics of being simple in production process, stable, easy in organization amplification, high in product yield, low in equipment investment cost, low in production cost, high in comprehensive resource utilization rate and environmentally friendly. The sulforaphene prepared by the method has the characteristics of being high in purity, free of solvent residue and stable in performance, and can be widely used in the industries of medicine, food and daily chemical.

The invention discloses a pharmaceutical composition for treating diabetes mellitus and a preparation method thereof. The pharmaceutical composition is prepared from the following medicinal raw materials in a proportion: gomisin G, mucuna birdwoodiana, herba artemisiae adamsii, glucoraphanin, swertiamarin and sarmentosin. The pharmaceutical composition can be manufactured into various preparations according to a conventional preparation process, and has a remarkable curative effect for treating diabetes mellitus.

The invention discloses a method for preparing sulforaphene, and belongs to the technical field of separation and purification. The glucoraphanin hydrolysate is prepared according to the method disclosed by the patent with the patent number of 201510270292.5, and then a sulforaphene crude separation solution is prepared, a TBP or TRPO sulfonated kerosene extraction solution is prepared, a sulforaphene primary purification solution is prepared, a purified sulforaphene concentrated solution is prepared and finally the sulforaphene is prepared, wherein the purity of the sulforaphene product is 99.14%. The method is simple and convenient to operate, accurate in separation, mild in condition, energy-saving and low in production cost, the extraction agent can be recycled, the product is stable, the purity is high, the byproducts generated in the production process are effectively utilized, no 'three wastes' is generated, and the method is a typical green production process, so that the problems that the sulforaphene is easily rearranged or isomerized in water, and the product is not easy to store for a long time are solved. The sulforaphane product prepared by the method can be widely applied to the fields of health products and medicines.

The invention discloses a breeding method of a broccoli variety with high glucoraphanin content in broccoli florets and seeds. According to the invention, a broccoli double haploid (DH) line obtainedby microspore culture is used as a male parent, a cytoplasmic male sterile line of a high-glucoraphanin-content broccoli variety obtained by multi-generation backcross transformation is used as a female parent, and sexual hybridization is performed to obtain the novel high-glucoraphanin-content broccoli variety. According to the high-glucoraphanin-content broccoli variety obtained through breedingin the invention, the content of glucoraphanin in the broccoli florets is 2.5 times or above the content of glucoraphanin in a common variety (a commercially-available variety with the highest glucoraphanin content), the content of glucoraphanin in the broccoli seeds is 107 micromole / g or above, and the broccoli seeds do not contain alkenylated glucosinolate components.

The invention provides a method for preparing reduced type glucoraphanin by enteric bacterium metabolism and a detection method. The method comprises the following steps: preparing an anaerobic culture solution and an intestinal bacteria solution, mixing and incubating the anaerobic culture solution and the intestinal bacteria solution to obtain an intestinal bacteria culture solution, then addinga glucoraphanin extract into the intestinal bacteria solution, thus obtaining a crude product solution containing metabolite under the action of intestinal flora and utilizing sephadex to separate and purify the metabolite to obtain the highly purified reduced type glucoraphanin. The reduced type glucoraphanin obtained by the invention has high degree of purity and a good purification effect.

The invention discloses a foliar fertilizer capable of simultaneously increasing the contents of thioglycoside and selenium in vegetables, and an application method thereof. When the foliar fertilizeris applied, the concentration of sulfur is 1-4 mM, and the concentration of the selenium is 30-150 MuM. The application frequency of the fertilizer is 7 days, and the application means includes dissolving the foliar fertilizer in water and then applying the liquid to leaf surfaces. Through application of the foliar fertilizer, the content of the total thioglycoside, the content of glucoraphanin and the content of the selenium in cabbages can be effectively increased by 16.4-47.2%, 48.2-84.0% and 6.8-40 times respectively, and the content of the glucoraphanin and the content of organic selenium in cauliflowers can be increased by 27.9-89.5% and 5.1-16.5 times respectively.

The invention provides a method for preparing glucoraphanin from glucoerucin and belongs to the technical field of biochemical pharmacy. The method overcomes the defects of high reagent dosage, residual solvents, complicated operation and technologies in a traditional process of preparing the glucoraphanin from the glucoerucin as well as high cost, energy loss and chromatographic column blockage in the presence of KCl after column chromatography isolation, thereby being high in practical application value and promising in large-scale industrial application prospect.

A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking is disclosed. Through a process of crossing, selfing and back-crossing in order, based on a molecular marking technique, and through combining marker assisted selection and traditional phenotypic selection, related gene fragments in a broccoli selfing line having a high glucoraphanin content are transferred to a cauliflower selfing line having excellent comprehensive properties to obtain the novel cauliflower variety having a high glucoraphanin content. By utilizing molecular marking, single plants having high glucoraphanin contents can be selected in a seedling stage and single plants having low glucoraphanin contents are eliminated, the area of land utilized is small, the working load is low, and disadvantages such as long cycles, high working loads and susceptibility to environment influences of methods for measuring glucoraphanin contents of ball-flowers in conventional breeding methods can be overcome. Different cauliflower selfing lines can be subjected to high-glucoraphanin-content creation so that passive glucoraphanin content breeding is turned into initiative breeding and creation, thus providing rich original materials for breeding the novel cauliflower variety having the high glucoraphanin content.

The invention discloses a glucoraphanin and moringa oleifera extract composition buccal tablet and application thereof, and belongs to the technical field of food. The moringa oleifera extract is obtained by uniformly mixing extracts of moringa oleifera leaves, moringa oleifera stems or moringa oleifera seeds with maltodextrin and then performing spray drying. The moringa oleifera buccal tablet comprises the following components in parts by weight: 60-80 parts of moringa oleifera extract, 5-10 parts of glucoraphanin, sodium carboxymethyl cellulose and / or citric acid and / or menthol and / or cane sugar and / or magnesium stearate, and the moringa oleifera buccal tablet is formed by extruding through a punching machine after mixing. The thermal stability of myrosinase in the moringa seed extract is higher than that of broccoli extract, and the moringa seed extract still has good stability at 85 DEG C. The glucoraphanin can be subjected to stable enzymolysis in the oral cavity and the digestive tract to generate the sulforaphane. The high-activity sulforaphane is provided for the human body.

The invention provides a composition comprising glucoraphanin and functional oligosaccharides. The invention also relates to the use of said composition in the manufacture of products for cancer chemoprevention and / or treatment.

The invention relates to a pharmaceutical composition for improving and treating leukotrichia and / or alopecia and a preparation method thereof. The pharmaceutical composition comprises glucoraphanin and myrosinase as effective components, based on the total weight of the pharmaceutical composition, the content of glucoraphanin is 0.1-50%, and the total enzyme activity of myrosinase is 0.1 mU to 10U. The pharmaceutical composition provided by the invention has the characteristics of safety, rapidness, effective improvement and treatment of leukotrichia and / or alopecia, wide subject application range and the like.

The invention provides a fertilizer for improving broccoli yield and quality and a preparation method thereof. The preparation method comprises the following steps: firstly, mixing porous micro-powderprepared from corn straw powder with cottonseed hull powder to obtain mixed powder, spraying and granulating the mixed powder and polyglutamic acid to prepare particles, pouring the particles into anaqueous solution prepared from an inorganic fertilizer component, mixed bacterial powder and a corn-enteromorpha clathrata mixed fermentation product, and carrying out dipping treatment, and finally,performing vacuum freeze-drying to obtain the fertilizer, the fertilizer is particularly suitable for broccoli planting, the broccoli yield is effectively increased, the content of glucoraphanin, vitamin C and the like of the mature broccoli is greatly increased, and high-quality broccoli is harvested.

The invention relates to a method for breeding high glucoraphanin content Brassica rapa. The method comprises A: introducing a gene which is lost in function in a donor material into a receptor material to be cultured so that a polymerized single plant is obtained, and B: systematically breeding the polymerized single plant to obtain high glucoraphanin content Brassica rapa, wherein the gene losing functions comprises at least one of BrAOP2.1, BrAOP2.2 and BrAOP2.3. The high glucoraphanin content Brassica rapa contains the protein losing functions and the protein losing functions comprises at least two of BrAOP2.1, BrAOP2.2 and BrAOP2.3. The method utilizes a molecular marker-assisted selection technique, saves a breeding cost, shortens breeding time and improves breeding efficiency.