Method for extracting sulforaphane and myrosinase magnetic microspheres

A technology of magnetic microspheres and myrosinase, which is applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of low yield and achieve the effects of high purity, improved extraction rate, and convenient operation

Active Publication Date: 2019-07-30
LESHAN NORMAL UNIV +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting sulforaphane and myrosinase magne

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of ferroferric oxide magnetic microspheres

[0033] Add 20g FeCl3·6H2O and 60g sodium acetate to 600mL ethylene glycol, after ultrasonic stirring for 5 minutes, then add 50g polyetherimide (PEI for short), and stir for 5 minutes. Then, transfer to the reaction kettle, react at 200°C for 24h, cool to room temperature after the reaction, wash the product three times with ethanol and water respectively, after magnetic field separation, dry the magnetic component at 60°C to obtain trioxide ferromagnetic microspheres.

[0034] 2. Preparation of myrosinase solution

[0035] Weigh 200g broccoli seeds, pour them into a mortar, add liquid nitrogen, grind into powder, add 500mL extraction buffer (0.2mol / LTris-HCl buffer, pH 7.5), stir for 3 minutes and filter with multi-layer gauze, The filtrate was centrifuged at a high speed for 20 minutes at 4°C, and the supernatant was collected, which was the crude extract of myrosinase. Add 2 times the volume of ethanol to...

Embodiment 2

[0047] The preparation method of myrosinase magnetic microspheres is the same as in Example 1, and the extraction method of sulforaphane is as follows:

[0048] (1) Weigh 500 grams of broccoli seeds, put them in a frying pan, set the temperature of the frying pan to 110°C, stir fry for 25 minutes, press the fried broccoli seeds with an oil press, remove the grease, and obtain oil dried cake;

[0049] (2) Crush the oil cake into small particles of about 10 mesh, put it in a soaking bucket, add 1.5 kg of distilled water to the bucket, stir at room temperature for 40 minutes, pour out the supernatant to obtain the first filtrate, and pour it into the bucket again Add 1 kg of distilled water to the mixture, stir at room temperature for 20 minutes, pour out the supernatant to obtain the second filtrate, combine the first filtrate and the second filtrate to obtain the glucoraphanin extract;

[0050] (3) Add 2.5 grams of immobilized myrosinase magnetic microspheres to the glucorapha...

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PUM

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Abstract

The invention discloses a method for extracting sulforaphane and myrosinase magnetic microspheres, the method comprises the following steps of:(1) heating and stir-frying broccoli seeds, and squeezingoil to remove grease to obtain an oil cake;(2) crushing the oil cake into small particles, soaking and extracting in water to obtain a sulforaphane extract;(3) adding myrosinase magnetic microspheresinto the sulforaphane extract, performing enzymolysis, and recovering the myrosinase magnetic microspheres by magnet adsorption to obtain sulforaphane extract;(4) extracting the sulforaphane by usingdichloromethane to obtain a dichloromethane extract containing the sulforaphane, and concentrating the dichloromethane extract;(5) dissolving the concentrated solution by using acetonitrile, pouringout supernatant, and concentrating to obtain concentrated solution; (6) dissolving the concentrated solution obtained in step (5) by using edible ethanol, pouring out supernatant, and concentrating toobtain the sulforaphane. According to the method, by taking the solidified myrosinase magnetic microspheres as a catalyst, the glucoraphanin is hydrolyzed into sulforaphane, and the purity of the sulforaphane is as high as 95%.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting sulforaphane and myrosinase magnetic microspheres. Background technique [0002] The seeds of broccoli, a popular vegetable, contain glucoraphanin, which is degraded by the enzyme myrosinase contained in the broccoli seed itself to sulforaphane, which is so far found in vegetables The active substance with the best anticancer effect, has a variety of physiological activities. Japanese nutritionist Professor Fujia Yoko and others have verified through experiments that sulforaphane has the effect of treating cancer; British scientists have found that sulforaphane will help people resist cancer cells and reduce the risk of cancer; Johns Hopkins in the United States The Brassica Chemooprotection Laboratory of the University School of Medicine found that sulforaphane can activate the body's own anti-cancer substances; Ota Tsuito ...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N11/14C12P11/00
CPCC12N9/2402C12N11/14C12P11/00C12Y302/01147
Inventor 陈封政陈建明李书华张利兵
Owner LESHAN NORMAL UNIV
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