2825results about "On/in inorganic carrier" patented technology

This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.

A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.

Hydrogels containing catalase co-immobilized with an analyte-sensitive enzyme such as glucose oxidase are disclosed. The hydrogels may be pH-sensitive, and preferably are thin and lightly crosslinked. The catalase is present in concentrations ranging generally from 100 units/ml to about 1000 units/ml. These hydrogels have much faster swelling response times as compared to hydrogels without catalase, and are useful in biosensors and analyte-responsive drug delivery devices. The hydrogels also have an increased useful life, due to protection of the immobilize analyte-sensitive enzyme from degradation by hydrogen peroxide.

Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

A mediator molecule is immobilized on the surface of a metallic or ceramic implant material. An anchor molecule such as a dialdehyde having a functional group that covalently binds the mediator molecule is covalently bound to the surface, and the mediator molecule is coupled to the functional group of the anchor molecule. The implant material may be composed of titanium, titanium alloy, aluminum, stainless steel or hydroxylapatite. Oxide units on the surface of the implant material can be increased preferably by treating with hot chromic-sulphuric acid for 0.5 to 3 hours at a temperature between 100 to 250° C. prior to binding the anchor molecule. Also, prior to binding the anchor molecule, the surface of the implant material can be activated by reacting with a silane derivative. Mediator molecules include BMP protein, ubiquitin and antibiotics, and the implant material may be an artificial joint or coronary vessel support such as a stent.

The present invention relates a graphene/metal oxide hybrid aerogel, a preparation method and applications thereof, and belongs to the field of nanometer material applications. The hybrid aerogel comprises a graphene network and a metal oxide network, wherein the two networks are mutually wound to form a hybrid aerogel, and the metal oxide network is further a crystalline state. The hybrid aerogel preparation comprises: preparing a graphene oxide organic solution, adding a soluble metal salt and an epoxide to obtain a uniform non-flowing hybrid wet gel, and carrying out drying and charring to obtain the graphene/metal oxide hybrid aerogel. The hybrid aerogel can be used as an energy storage material, an electromagnetic shielding material, a biological enzyme catalysis carrier and a CO2 absorption material, and has wide applications.

The present invention relates to a three-dimensional system, and compositions obtained therefrom, wherein individual layers of the system comprise channels divided longitudinally into two compartments by a centrally positioned membrane, and wherein each compartment can comprise a different cell type.

Modified surfaces, substrates, and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide either non-reactive surfaces or low density reactive groups, preferably on an otherwise non-reactive surface, for use in different applications including single molecule analyses.

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.

The invention discloses crack self-remediation regenerated concrete based on urease production microorganism mineralization deposition and a preparation method. The crack self-remediation regenerated concrete comprises components, namely, expanded perlite carried with urease production microorganisms, cement, stone, sand, silica fume, water, urea, calcium chloride, a urease production microorganism suspension and a water reducing agent. The urease production microorganisms are adopted as a concrete crack remediation agent, urease can be generated through metabolism through the urease production microorganisms, and the urea can be decomposed into NH4<+> and CO3<2+>, and furthermore the calcium carbonate can be mineralized and deposited to remedy cracks. In the preparation process, a part of the crack remediation agent is directly mixed with the concrete, then cracks and holes in regenerated crude aggregate self and weak adhesion areas between regenerated aggregate and a new cement stone base can be remedied, and thus the mechanical property of the regenerated concrete can be improved; meanwhile, the other part of the crack remediation agent is firstly adsorbed into an expanded perlite carrier and is further mixed into the concrete, then the crack self-remediation property of the regenerated concrete in the service period can be improved, and thus the anti-penetrability performance and the durability of the regenerated concrete can be improved.

A method of preserving organisms in viable form, the method comprising: suspending organisms in a solution of electrospinnable polymer; drawing droplets of said solution through a spinneret; applying an electrostatic field to said droplets under electrospinning conditions; so as to form fibers having a diameter no greater than about 5 μm within which distinct organisms are encapsulated in viable form.

Implantable materials having defined patterns of affinity regions for binding endothelial cells and providing for directed endothelial cell migration across the surface of the material. The affinity regions include photochemically altered regions of a material surface and physical members patterned on the material surface that exhibit a greater affinity for endothelial cell binding and migration than the remaining regions of the material surface.

The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.

A method for the preparation of an immobilized protein ultrathin film reactor by immersing a solid support alternately into an aqueous solution of protein, and into an aqueous solution of polyion charged oppositely to said protein and preparing a structurally controlled ultrathin film of mono- or multi- protein layers on a said solid support with precision at molecular level. And a method for chemical reaction of a substrate by preparing an immobilized protein ultrathin film reactor composed of multiple layers of protein on a solid support by above mentioned method, and initiating a chemical change of the substrate molecules using obtained immobilized protein ultrathin film reactor.