2870 results about "Mycelium" patented technology

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.

Compositions, methods and business applications of using new and recycled cardboard infused with a plurality of saprophytic (including endophytic) and mycorrhizal fungi matched with seeds of plants (including trees, vegetables, herbs and grasses) whereby the cardboard can be sprouted by end-users to start ecosystems. Such containers may have carbon-credit value for companies and consumers when planted and grown as a carbon sink or carbon offset for the photosynthetic and mycelial sequestration of carbon dioxide. The relative weight of the Life Box's added seeds and spores does not significantly affect the total weight of the infused cardboard, thus not increasing transportation costs.

The invention discloses a method for producing a liquid strain of edible mushrooms. The method for producing the liquid strain of the edible mushrooms comprises the steps that (1) the liquid strain of the edible mushrooms is inoculated in a PDA culture medium to be activated, and separation is conducted after cultivation so that an activated mycelium can be obtained; (2) the activated mycelium is inoculated in a solid-state saw dust culture medium to be cultivated for 28-32 days so that a recovery culture 1 composed of the mycelium and the solid-state saw dust culture medium can be obtained; (3) the recovery culture 1 is smashed into particles with the diameters ranging from 20 meshes to 40 meshes and then the particles are laid flat in a shallow plate with a thickness of 3 m-4m to be cultivated for 2-3 days in a sealed mode so that a recovery culture 2 can be obtained; (4) after the recovery culture 2 is smashed into small blocks with the diameter not larger than 0.5 cm, pulping is conducted by adding water to the small blocks, and then the obtained pulp and the agar solution with the concentration ranging from 0.15% to 0.2% are made to be in contact and evenly mixed. According to the method for producing the liquid strain of the edible mushrooms, operation is easy, a complicated cultivation device is not needed, and the production efficiency is high. The liquid strain, obtained according to the method, of the edible mushrooms has the advantages of being stable in quality, robust, slow in degeneration and easy to store and transport.

The invention relates to a strain Pleurotus ostreatus TIB.BPE.10010 with the accession number being CGMCC No.6232. The invention also relates to a method of preparing L-erythrothioneine. The method comprises steps of culturing and fermenting the Pleurotus ostreatus CGMCC No.6232 to perform biosynthesis to form the L-erythrothioneine and extracting the L-erythrothioneine from mycelium cells.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention discloses a preparation method of a mushroom ferment beverage with effect of improving immunity, and belongs to the technical field of food beverages. According to the method disclosed by the invention, mushrooms and/or medicinal plants, fruit and ginseng are adopted as raw materials. The method comprises the following steps: slicing, chopping or crushing the raw materials; uniformly mixing the sliced, chopped or crushed raw materials so as to obtain a mixture; after adding sugar in the mixture, fermenting the mixture in ferment microorganisms; adding sugar when the sugar content is lowered; continuing fermentation so as to obtain the mushroom ferment beverage. According to the method disclosed by the invention, ferment bacteria can regulate intestinal flora and generate SOD (superoxide dismutase), so that the raw materials can be successfully fermented; the prepared ferment beverage contains protease, lipase, SOD, pectinase, lucid ganoderma, cauliflower fungus, pleurotus eryngii polysaccharide, rare ginsenoside, Rb2, C-K and the like. According to the method disclosed by the invention, the fermentation liquor and mycelium of the mushrooms can be directly used for fermentation; compared with sporophore, the raw materials are easier to obtain, and the method has the advantages of being short in fermentation cycle, easy in formula, simple in fermentation process, and the like.

The invention discloses a stropharia rugoso-annulata cultivation method. The method comprises the following steps of season arrangement, cultivation material selection, site selection, material collection, cultivation material treatment, sowing and mycelium cultivation, spawn running period management, earthing method and management after earthing, fruiting period management, harvesting, pest and disease control and processing. The method has the advantages that the stropharia rugoso-annulata cultivation method fully utilizes the straw resource, the specific steps and conditions of cultivation are particularly limited, and the yield of stropharia rugoso-annulata can be effectively increased; meanwhile, by adjusting the cultivation proportion of different kinds of straw, growth and development of the stropharia rugoso-annulata can be effectively controlled, the stropharia rugoso-annulata is high in yield, excellent in quality and capable of adapting to the market requirement.

A bioadsorbent for treating the sewage containing heavy metal ions (5-2000 ppm) and with 2-11 of pH value is prepared by coating method and blotting method to coat the surface of mycelia (citrobacteria, yeast, etc) with the glucosan, chitosan, or chitosan derivative. Its advantages are low cost, high adsorption capacity (30-100 mg/g) and cyclic use (20 times).

The invention belongs to the fields of microbe application technologies and food biotechnologies, and discloses a strain used for fermenting rice bran and wheat bran extracts for producing grifolan. The grifola frondosa strain is collected in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan, China on Aprial 7th, 2011, and has a strain collection number of CCTCC No: M2011113. The name of the strain is Grifolasp. JSU10-2. According to the invention, through protoplast laser mutation, the strain with high yield of mycelium polysaccharide produced from cheap raw materials is obtained. When the strain and an original strain are respectively used in liquid fermentation of a rice bran and wheat bran composite culture medium, the dry weight and polysaccharide of the mutant strain are respectively increased by 31.7% and 32.6% compared with those of the original strain.

The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.

The invention relates to the field of microorganisms, in particular to a cultivation method of cordyceps militaris. The cultivation method can produce a large quantity of cordyceps militaris rhzomorph. The cultivation method comprises respectively preparing a slope culture medium, a liquid seed culture medium and a sporocarp cultivation culture medium; vaccinating cordyceps militaris cultures on the slope culture medium and cultivating at 23-25 DEG C for 6-8 days to obtain slope cultures; vaccinating the slope cultures on the liquid seed culture medium and conducting shake cultivation at 23-25 DEG C for 2-3 days at the speed of 250 circles/minute to obtain seed liquid; vaccinating the seed liquid on the sterilized sporocarp cultivation culture medium and conducting dark cultivation in a disinfected light-shielding culture room to obtain mycelium; conducting coloring cultivation on the mycelium and obtaining mycelium with buttons; and conducting sporocarp cultivation on the mycelium with the buttons to obtain the cordyceps militaris rhzomorph.

The invention discloses a culture medium and method for submerged fermentation of inonotus obliquus, relating to a culture medium and method for microbial submerged fermentation, and solving the problems of low yield of traditional inonotus obliquus submerged fermentation mycelia and low output of intracellular polysaccharide and extracellular polysaccharide of the inonotus obliquus as the activeingredient for the submerged fermentation. The culture medium for submerged fermentation is prepared from the following components: carboxymethyl cellulose, glucose, soybean meal, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and the balance of water. The method for the submerged fermentation comprises the following steps of: (1) preparing a culture medium for the submerged fermentation of inonotus obliquus; (2) inoculating the inonotus obliquus; and (3) carrying out submerged fermentation at two stages. By means of the culture medium and the method for the submerged fermentation of the inonotus obliquus in the invention, higher mycelium yield and intracellular and extracellular polysaccharides can be obtained. The mycelia polysaccharide obtained by the invention is 1.4 times higher than that of wild fruiting bodies, and the content of other active ingredients is higher than or equal to that of the wild fruiting bodies. The culture medium and method provided by the invention can be used for providing a rapid and abundant seed liquid for the large-scale artificial culture of the inonotus obliquus.

The invention provides a method for interplanting ganoderma lucidum in tea gardens. The method includes the steps that ganoderma lucidum bacterial strains which can adapt to strong light for growth and appropriate compost are selected, compost mixing, bagging, sterilization, inoculation and mycelium culture are conducted, and after bags are filled with mycelia and colors of the mycelia are changed, ganoderma lucidum wild imitation substitute earthing cultivation is conducted in a tea garden ecological environment. By the adoption of the novel method for cultivating the ganoderma lucidum, the quality of the ganoderma lucidum can be improved, conventional greenhouse investment for substitute earthing ganoderma lucidum cultivation is reduced, the operating process is simplified, the economic output value of the tea gardens is increased, soil fertility of the tea gardens can be also improved, the yield and the quality of tea leaves are improved, production and income of the tea gardens are promoted, and the method has remarkable economic and social benefits and is suitable for being massively popularized and applied.

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

The invention discloses a preparation method of an edible mushroom culture medium. The preparation method comprises the following steps: extruding a grain material into cylindrical particles with diameters of 2-4mm and lengths of 5-10mm, then uniformly mixing with a straw material, adding calcium carbonate and lime powder, adding clear water to adjust the water content while stirring, adding tap water after uniformly and fully mixing all the materials, and finally enabling the water content to reach 64-67 percent and pH to reach 8-10, wherein the grain material is one or more of bean pulp, corn kernels, bran and rice bran; and the straw material is one or more of wood flour, corn kernels, bagasse and cotton seed hull. Compared with a conventional culture medium, the culture medium provided by the invention has the advantages that the mycelium culture period and the fruiting period are shortened, mycelium is thicker and stronger in, and the yield is stably improved.

A solid culture method for antrodia camphora includes culturing mycelia with space bags and cultivating sporophores in air. The cultured product and its usage are also disclosed.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The present invention provides a method for the preparation of a myceliated cacao bean or other agricultural product. This method includes providing cacao beans or other agricultural substrate, optionally hydrating the cacao beans or other agricultural product, and optionally pasteurizing or sterilizing the cacao beans or other agricultural substrate to provide prepared cacao beans or other agricultural substrate, and a step of inoculating the prepared cacao beans or other agricultural substrate with a prepared fungal component and culturing the inoculated cacao beans or other agricultural substrate to prepare the myceliated product. The methods of the instant invention result in prepared cacao beans or other agricultural substrate having reduced levels of undesirable taste components, such as theobromine, catechin, epicatechin, gallic acid equivalents, and/or 2-methoxy-3-isopropylpyrazine, and increased levels of myceliation products, such as fungal β-glucans, chitin, proteins, glycoproteins, pyrazines and polysaccharides, relative to starting cacao beans or other agricultural substrate.

The invention provides a preparation method of ganoderma lucidum mycelium slices, which can be used for solving the technical problem that ganoderma lucidum mycelium powder is inconvenient to eat in the prior art. The preparation method comprises the steps of preparation of a culture medium, activation of strains, inoculation and culture, baking, puffing, crushing, mixing, granulating and tabletting, wherein the preparation of the culture medium comprises the steps of: A, preparation of a grain culture medium; B, preparation of a slant culture medium; C, preparation of a shake flask culture medium; D, preparation of a fermentation tank culture medium. The preparation method can be widely used in the processing of the ganoderma lucidum mycelium slices.

The invention relates to a method for culturing pleurotus eryngii. The method for culturing pleurotus eryngii comprises the steps of: preparation of a culture medium, bagging of nourishment, sterilization and cooling, vaccination, mycelium culture, culture management, pest control, pickup management and treatment after pickup. The pleurotus eryngii culturing method disclosed by the invention enables the pleurotus eryngii planting not to be limited by natural environmental condition, and can realize a higher yield.