112 results about "Mycelium" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The application discloses a bucket type cultivation method for overcoming continuous cropping obstacles of bamboo shoots. Through the bucket type inoculation, the mycelium is effectively isolated from the miscellaneous bacteria and pathogenic microorganisms in the soil before the mycelium completes the dominant expansion, the mycelium expansion stability is obviously improved, and the mycelium death and yield loss caused by the continuous cropping obstacles are avoided. Field tests show that the yield of the second year can be recovered to more than 90% of the yield of the first year by using the method, and the yield of the second year is less than 10% of the yield of the traditional reseeding mode, and almost no yield is obtained, so the method has good stability and generalizability.

The present application relates to the technical field of microbial fermentation, in particular to a polyporus arctorus and a culture method and application thereof. A polyporus arctorus YH-DB-1 is screened in the present application, and a large amount of glycopeptide can be extracted from the mycelium of the strain by culturing the strain, the peptide content in the glycopeptide can reach more than 45%, and the present application solves the problem of low peptide yield in the existing polyporus arctorus glycopeptide.

This invention relates to the field of *Glechoma heliotropium* cultivation technology. It relates to a soilless cultivation method for *Glechoma heliotropium*, using oak sawdust, wheat bran, corn flour, glucose, magnesium sulfate, potassium dihydrogen phosphate, and gypsum as culture medium raw materials to prepare mycelium logs. *Glechoma heliotropium* is inoculated into bagged culture medium and cultured under suitable conditions to achieve stable fruiting. This invention allows for the large-scale production of *Glechoma heliotropium* fruiting bodies, which can be promoted and applied as a natural functional food, and the active substances can be extracted for use in health products or pharmaceuticals.

This invention discloses a bio-composite matrix for solid waste and yellow sand systems, its preparation method, and its application. Belonging to the technical field of bio-composite organic matrices, this invention utilizes the significant complementarity of fly ash, drum slag, and biological organic matter in terms of physical structure, chemical composition, and biological activity. Through compounding, an ecological restoration matrix with aeration, water retention, and nutrient supply capabilities can be constructed. Fly ash improves the matrix's pore distribution, enhancing permeability and water retention capacity. Simultaneously, its rich content of minerals such as Si, Al, and Ca can regulate the matrix pH and provide nutrients to a certain extent. Drum slag acts as a skeletal support, improving the composite matrix's mechanical strength and compressive strength, preventing matrix compaction and sedimentation. Biological organic matter, rich in organic matter, humus, and residual mycelium, possesses excellent water retention and slow-release nutrient capacity, significantly enhancing matrix fertility and microbial activity, providing carbon sources and nutritional support for plant root growth.

This invention discloses a pure edible fungus mycelial fiber material, its preparation method, and its applications. The preparation method includes the following steps: S1, fungal activation; S2, liquid static film formation; S3, semi-solid thickening; S4, solid cultivation and environmental induction for densification and solidification: the thickened mycelial layer is peeled off and transferred to a solid cultivation environment containing a support carrier, inducing the mycelium to densify and solidify, obtaining a pure mycelial fiber dense layer; S5, peeling and post-treatment; S6, hot pressing to obtain the pure edible fungus mycelial fiber material. This invention completely eliminates the limitations of agricultural waste substrates on material performance. The obtained pure mycelial material has a complete mycelial skin morphology, high density, and certain tensile strength, and can be used to prepare imitation leather materials, decorative finishes, etc., possessing high application value.

The utility model provides a kind of edible mushroom multilayer three-dimensional cultivation frame, it is specifically related to edible mushroom technical field, it is characterized by: two groups of chain rotation tracks are symmetrically arranged in the inside of cultivation support frame two sides, and the surface of two groups of chain rotation tracks is fixedly connected with multiple fixed racks, each fixed rack is connected with fixed shaft, and the other end of each fixed shaft is fixedly connected with support frame top, support platform is horizontally arranged in support frame, and rotating shaft is connected in the both ends of support platform, and the both ends of rotating shaft are connected with support frame, and every two symmetrical support frames are connected with a support platform, and every support platform is parallel, and cultivation box is arranged on the surface of support platform;The utility model is through the synergistic effect of chain rotation track and gear transmission, and support platform can be circularly displaced along rotation direction, and different growth stages of mycelium can be observed in time, effectively solve the problem that ventilation is blocked in later stage of fungus stick, and uniform illumination radiation covers each layer of mycelium, and the quality of mycelium development is significantly improved.

The utility model is applicable to the technical field of poria cocos fungus bag planting, and provides a fixing clamp device for poria cocos fungus bag planting, which comprises a connecting frame, and mounting plates are symmetrically and fixedly mounted on the connecting frame; the clamping ring is assembled on the mounting plate, a clamping mechanism is arranged between the clamping ring and the mounting plate, and the clamping mechanism is used for driving the clamping ring to move to clamp pine sections used for poria cocos fungus bag planting; the fixing band is assembled on the mounting plate and is used for fixing the poria cocos fungus bag on a pine section; positioning columns are arranged on the two mounting plates, connecting rings are fixedly mounted at the two ends of the fixing belt, and the two connecting rings are connected with the two positioning columns in a matched mode. According to the fixing clamp device for poria cocos fungus bag planting, poria cocos fungus bags and pine sections can be clamped and positioned, the fitting degree of the poria cocos fungus bags and the pine sections is enhanced, hyphae can better breed on the pine, and the poria cocos planting effect is improved.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

The utility model relates to stirring device technical field, especially edible fermentation with stirring device of edible fungus, including inside hollow jar body, the jar body outside is provided with feed inlet and discharge gate, be provided with motor on the jar body, the pivot that is in the motor output end and extends to the inside of jar body and the paddle that is in the pivot periphery, the paddle is made of swellable material, through the swelling morphological change of control paddle can match the shear force required in different stages of edible fungus fermentation in real time, both protect the mycelium and guarantee the stirring effect, the control valve is arranged on the pivot and exposes in the side of jar body outside, the inside hollow area of pivot and swellable chamber in the paddle inside communicate, can control fluid in and out through the opening and closing state of control valve and the fluid supply device outside, realize the control of paddle swelling morphological, more convenient and faster reach the shear force required in the current stage.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The present application provides a kind of auricularia auricular polypeptide and preparation method and antihypertensive application, belong to the field of biotechnology.The first aspect, the present application provides a kind of auricularia auricular fruit body, xiao zhen gu mycelium composite edible fungus polypeptide product;Second aspect, the present application also provides a kind of auricularia auricular fruit body, xiao zhen gu mycelium composite edible fungus polypeptide preparation method;Third aspect, the present application also provides a kind of auricularia auricular fruit body, xiao zhen gu mycelium composite edible fungus polypeptide in the application of preparation for preventing or treating hypertension and / or antifatigue effect drug.

The application relates to the technical field of edible fungus inoculation, and in particular to a chondrostereum bag inoculation device, which comprises a supporting frame and an inoculation device, the inoculation device comprises an inoculation box fixedly connected to the top of the supporting frame; two sealing plates are arranged in the scheme; after a fungus bag enters the inoculation box, the sealing plates are lowered to the bottom to form a completely closed space, the inoculation process is isolated from the open environment, bacteria spores (such as penicillium and aspergillus) in the air can be avoided from falling into the opening of the fungus bag or the inoculation position, the inoculation needle is made of soft silica gel material, can be slightly deformed with pressure when being inserted into the fungus bag, rigid extrusion and scratch of chondrostereum mycelium caused by traditional metal needles are avoided, the electromagnetic valve and the spiral blade are matched with the elastic sealing valve to send the seed cylinder, quantitative conveying is realized, growth differences caused by too much inoculation (intense nutrition competition) or too little inoculation (mycelium is difficult to quickly occupy space) can be avoided, strain waste caused by excessive inoculation is avoided, and the raw material cost can be significantly reduced in the long term.

The application discloses a ganoderma lucidum hydrophobin Glhyd694 gene, an encoded protein and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the ganoderma lucidum hydrophobin Glhyd694 gene is shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The ganoderma lucidum hydrophobin has self-assembly characteristics, can perform hydrophobic modification on a material surface, and has excellent emulsification activity and emulsion stability. In the ganoderma lucidum, the protein also participates in tolerance regulation of mycelium to various environmental stresses such as cell wall stress and oxidation stress. The application provides a new gene resource for development of natural surface modified materials and biological protective agents.

The present application relates to the technical field of medical image processing and intelligent auxiliary diagnosis, and particularly discloses a corneal fungal infection diagnosis method and system based on multi-modal time sequence information, which comprises the following steps: acquiring an image sequence and clinical data; inputting the image sequence and the clinical data into a multi-modal fusion analysis model, which extracts spatial form and space-time dynamic characteristics of the image and a clinical feature vector; fusing the two types of features to generate diagnosis features; and finally outputting a diagnosis result containing mycelium existence determination and judgment basis. The present application has higher diagnosis accuracy, stronger robustness, and good interpretability and interactivity, and simulates and enhances the comprehensive diagnosis thinking process of doctors by fusing space-time dynamic information of the image sequence and patient clinical context.

The application provides a fluorescent image recognition system for detecting fungi based on a deep learning algorithm, comprising an image acquisition module, a single spore and mycelium recognition module and a mycelium cluster recognition module; the image acquisition module is used for obtaining a target fluorescent image; the single spore and mycelium recognition module and the mycelium cluster recognition module are used for inputting the target fluorescent image into a pre-established deep learning algorithm model, recognizing single spores or mycelia and mycelium clusters through the pre-established deep learning algorithm model, and outputting fungal negative or positive results according to the recognition results. Compared with the prior art, after the image acquisition module obtains the target fluorescent image, the target fluorescent image is input into the pre-established deep learning algorithm model for automatic analysis, and the analysis of spores / mycelia and mycelium clusters that are different in morphology is divided into two modules for processing, so that the spores, mycelia and mycelium clusters can be more accurately recognized and distinguished.

The utility model discloses a kind of segment wood tremella cultivation punch inoculation device, belong to segment wood tremella cultivation technical field.A kind of segment wood tremella cultivation punch inoculation device, including rack and the positioning table of fixed installation on rack, fixed installation is installed on rack with mounting bracket, fixed installation is installed on mounting bracket with the control box for drilling and inoculation, further including slidingly installed on control box with sliding plate, fixed installation is installed with the limiting piece for adjusting drilling depth on sliding plate, fixed installation is installed with positioning plate on movable plate, slidingly installed with adjusting plate in positioning plate, fixed installation is installed with positioning column on adjusting plate, when adjusting plate moves, the distance between positioning column and control box changes;The utility model uses positioning plate, adjusting plate and positioning column cooperation, effectively promote the accurate degree of distance between adjacent inoculation hole, adjusting plate can move simultaneously, and then can adjust the distance between adjacent inoculation hole according to demand, it is convenient to adapt different inoculation hole drilling requirement.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The application discloses a bat moth lepidoptera cordyceps strain and application thereof in preparation of anti-neutron radiation medicines. Samsoniella hepiali The strain is the bat moth lepidoptera cordyceps strain P969 (Pleurotus spodoleucus), which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 20251172 and a preservation date of May 26, 2025. Mycelium extracts of the strain can protect biological tissues against neutron radiation and repair organ damage caused by neutron radiation, so that the strain has a wide development space and a good development and application prospect.

The present application relates to mycelium culture technical field, especially edible mushroom automatic detection culture device, including incubator, the top inner wall of incubator is provided with infrared plant growth lamp, and its outer wall is connected with the sliding door, the inner wall of sliding door is provided with culture base, the humidity adjusting mechanism is set, when the culture base inside culture solution content reduces, the piston can enter the cylinder in the bottom of first water tank, then through first spray head, the culture solution is supplemented to the mycelium root, and through the extension of first electric push rod, the spray amount of first spray head can be adjusted in a certain range, the humidity of mycelium root is controlled, when the mycelium growth environment is too dry, the clean water in second water tank is sprayed to the incubator through second spray head, so that the mycelium growth environment is humidified, and the environmental humidity and root culture solution content are controlled according to the actual growth needs of mycelium.

The present invention relates broadly to methods of producing Mycelium-based materials, to Mycelium-based products, and industrial applications thereof. Non-limiting example embodiments of the present invention aim to enhance the production process of a mycelium-based composite by employing a combination of specific mechanical, chemical and manufacturing processing techniques to improve the bonding between the mycelium hyphae and the substrates and / or to enhance the microstructure of the final composite / product and / or to enhance production efficiency of final composite / product.

The application discloses a Ganoderma sinense triterpene extract, a preparation method thereof and anti-aging application. The application adopts liquid static fermentation to obtain Ganoderma sinense mycelium, extracts the mycelium after freeze-drying of the mycelium with an organic solvent, adds activated carbon for oscillation decolorization, further concentrates, adds water, and dries the precipitate part to obtain a C-3 β triterpene extract rich in multiple C-3 The extract has excellent anti-aging activity, can be added as a functional ingredient raw material in cosmetics, and has a broad market prospect.

The application discloses an improved PDA culture medium for Agaricus bisporus mother culture and application and belongs to the technical field of edible mushroom cultivation. The improved PDA culture medium for Agaricus bisporus mother culture is based on a PDA culture medium and is added with Agaricus bisporus residue and peptone or beef extract. The culture medium provided by the application can significantly promote the growth of Agaricus bisporus mycelium, shortens the mother culture time from 23-25 days of the traditional method to about 13-15 days, greatly improves the strain production efficiency, and reduces the production cost and pollution risk.

The present invention relates to a method for manufacturing a mushroom mycelium leather sheet using shiitake mycelium and relates to a method for manufacturing a mushroom mycelium leather sheet that exhibits color and texture similar to animal leather by inducing the curing phenomenon of a shiitake mushroom.

This invention provides a method for preparing fermented pig feed based on bamboo powder and mycelium residue, belonging to the field of livestock and poultry feed preparation technology. Using bamboo powder and mycelium residue, a byproduct of edible fungi cultivation with bamboo powder as the core substrate, as raw material, the invention achieves efficient degradation of lignocellulose and bioconversion of nutrients in the bamboo powder and mycelium residue through core steps such as enzymatic pre-cell wall disruption pretreatment, segmented solid-state fermentation with compound functional microbial agents, and precise nutrient compounding. The resulting fermented pig feed is low in crude fiber, high in crude protein, palatable, and rich in bioactive substances. It can replace 10%-30% of traditional corn-soybean meal diets, significantly reducing feed costs for pig farming, improving pig growth performance and pork quality, and simultaneously constructing a closed-loop circular economy model of bamboo-fungi-livestock, possessing significant economic, ecological, and social benefits.

This invention relates to a strain of *Sanghuang* fungus suitable for making fermented barley tea and its applications, belonging to the field of biotechnology. The *Sanghuang* fungus strain described in this invention was deposited on April 3, 2024, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41280. This strain is suitable for solid-state fermentation of barley grains for making barley tea. The mycelium of this strain can penetrate the interior of barley grains, fully converting and utilizing the nutrients in the barley grains, exhibiting characteristics such as rapid growth and good appearance. The roasted *Sanghuang* barley tea has a yellow and lustrous color, overcoming the drawback of traditional barley tea being prone to causing "heatiness," possessing both the mellow aroma of barley tea and the unique refreshing aroma of *Sanghuang* fungus, and containing medicinal components of *Sanghuang* fungus. The *Sanghuang* fungus mycelium formed by fermentation and culture of this strain using barley grains as nutrients effectively solves the shortcomings of *Sanghuang* fungus being difficult to cultivate and expensive, while greatly increasing the added value of traditional barley tea.

The application belongs to the technical field of food and medicine, and particularly relates to a cordyceps militaris polysaccharide, a preparation method and application thereof. The application provides a method for improving the yield of cordyceps militaris polysaccharide by optimizing a liquid fermentation process, and proves that the cordyceps militaris polysaccharide has a good blood glucose lowering effect on T2DM mice. The application provides a liquid fermentation method capable of significantly improving the yield of cordyceps militaris polysaccharide, and the yield of the cordyceps militaris polysaccharide is improved to 82.7 mg / g mycelium, and the culture time is short. The cordyceps militaris polysaccharide obtained has a clear key structure characteristic range. The cordyceps militaris polysaccharide obtained has a good blood glucose lowering effect on T2DM mice, and by remodeling the intestinal flora, improving the SCFAs level, and improving a plurality of high blood glucose related indexes, the T2DM related high blood glucose symptoms are relieved.