1090 results about "Mycelium" patented technology

The invention relates to fusarium venanii with high protein conversion rate and application of the fusarium venanii to improvement of hypha protein satiety, and belongs to the field of genetic engineering technology and food biotechnology. The fusarium venenatum FCPD disclosed by the invention is preserved in the China Center for Type Culture Collection on December 6, 2024, and the preservation number of the fusarium venenatum FCPD is CCTCC (China Center for Type Culture Collection) NO: M 20242752. The glucose consumption of a substrate for producing mycelia with the same dry weight by the fusarium venanii is reduced by 40-60% compared with that of a wild type, the protein conversion rate is improved by 70-80%, the grease content is improved by 90-110%, the gastric semi-emptying time of in-vitro simulated digestion is prolonged by 10-30%, and the quality of fusarium venanii mycelium protein is remarkably improved. Genetic engineering modification work can be further developed, and fusarium strains with high substrate conversion rate, high grease content and high satiety potential can be cultivated.

The present invention relates to a preparation method of a pine mushroom mycelium fermentation product, and its products and applications, including: inoculating pine mushroom liquid seeds into a culture medium for culturing, solid-liquid separation, obtaining pine mushroom mycelium and fermentation filtrate; subjecting the fermentation filtrate to alcohol precipitation treatment to obtain pine mushroom mycelium crude polysaccharide; inoculating fermentation bacteria seed liquid into a fermentation medium added with pine mushroom mycelium and pine mushroom mycelium crude polysaccharide for culturing to obtain a fermentation liquid; centrifuging the fermentation liquid, filtering the supernatant and sterilizing to obtain the pine mushroom mycelium fermentation product. The present invention develops a new fermentation product with pine mushroom mycelium as a fermentation substrate, the fermentation product has excellent soothing and antioxidant effects, and compared with the method of fermenting a single pine mushroom mycelium or a single pine mushroom mycelium crude polysaccharide, the fermentation product of the present invention has better soothing and antioxidant effects.

The invention relates to a phellinus igniarius mycelium fermentation product and a preparation method and application thereof, and the preparation method comprises the following steps: inoculating a phellinus igniarius mycelium seed solution into a sterile fermentation culture medium for culturing, carrying out solid-liquid separation, filtering a clear liquid through a membrane and sterilizing to obtain the phellinus igniarius mycelium fermentation product, the fermentation medium comprises the following components: a beet extract, a deep eutectic solvent and water. The beet extract and the deep-eutectic solvent are creatively adopted as culture medium components to prepare the phellinus igniarius mycelium fermentation product, the beet extract and the deep-eutectic solvent jointly provide nutrients needed by phellinus igniarius fermentation, meanwhile, the deep-eutectic solvent is used for regulating metabolism, and compared with a product obtained through fermentation of a traditional culture medium, the phellinus igniarius mycelium fermentation product has the advantages that the fermentation efficiency is high; the content of active ingredients in a fermentation product obtained by adopting the culture medium with the formula is remarkably increased, and the fermentation product is good in uniformity and high in stability, is a clear liquid in appearance, has no abnormal odor and has slight phellinus igniarius characteristic odor.

The invention relates to the technical field of fermentation of edible and medicinal fungi, and discloses a phellinus igniarius fermentation method capable of producing high-yield Hispidin and an application of the phellinus igniarius fermentation method. The phellinus igniarius fermentation method comprises the following steps: inoculating a phellinus igniarius strain into a culture medium containing a copper ion compound, and fermenting. The phellinus igniarius mycelium obtained by adopting the fermentation method is rich in Hispidin, and can be widely used for preparing related medicines and health foods for protecting liver, resisting tumors, resisting viruses, resisting oxidation, reducing uric acid and the like. In addition, the invention also discloses a high performance liquid chromatography detection method for analyzing the content of Hispidin, by adopting the method, the content of Hispidin and precursor substances (such as protocatechuic acid, protocatechualdehyde, caffeic acid, osmundone and the like) in the phellinus igniarius leavening and derivative products thereof as well as phellinus igniarius mycelia can be effectively analyzed, and the accuracy of Hispidin detection is favorably improved.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention discloses a conditioner for realizing rapid granulation of aerobic granular sludge and improving settleability, relates to the field of aerobic granular sludge, and aims to solve the problems of low AGS formation speed, poor AGS stability and low settleability caused by low aeration rate. The conditioner is composed of mycelium pellets, mature aerobic granular sludge, a biological flocculant and activated carbon; the adding proportion of the conditioner is as follows: the mycelium pellets and the mature aerobic granular sludge are added according to the proportion that V mycelium pellets / V30min settled sludge is 20%-50% and VAGS / V30min settled sludge is 20%-50%, the adding amount of the biological flocculant is 0.2%-1% of the effective volume of a reactor, and the adding amount of the activated carbon is 0.2-0.8 g per liter of the effective volume of the reactor. A low aeration strategy is adopted, energy consumption is reduced, and better energy-saving and environment-friendly effects are achieved. According to the method, a large amount of granular sludge can be induced to be formed in only 17 days, basic granulation is realized in 25 days, and the formed aerobic granular sludge is compact in structure, regular in form and high in stability.

The invention provides a self-assembled hypha flexible carbon electrode material and a preparation method and application thereof, and belongs to the technical field of battery packs. The preparation method of the self-assembled hypha flexible carbon electrode material comprises the following steps: treating basswood with white rot fungus mycelia, crushing and roasting to obtain porous basswood / hypha composite carbon powder; and putting the porous basswood / hypha composite carbon powder and a carbon nanotube into a sterilized conical flask containing a potato glucose culture solution, inoculating gloeophyllum trabeum, performing activation culture, adding a dopamine hydrochloride aqueous solution, performing polymerization after ultraviolet irradiation, taking out and tabletting to obtain the self-assembled hypha flexible carbon electrode material. In the preparation process of the self-assembled hypha flexible carbon electrode material, the flexible carbon electrode material is synthesized by taking porous basswood / hypha composite carbon powder as a matrix, taking gloeophyllum trabeum hypha as an adhesive, taking polydopamine as a reinforcing agent and adding carbon nanotubes as a conductive reinforcing material, so that the technical problem of poor cycle performance of a lithium ion battery is solved.

The invention provides a culture method for increasing the content of a bioactive substance Emodic acid in talaromyces purpurascens, and relates to the technical field of medicinal fungus culture. The culture method of the homopurple talaromyces spp. Comprises the following steps: carrying out liquid culture on hyphae of the homopurple talaromyces spp., and then inoculating the hyphae into a liquid culture medium containing black bean powder for culture. The defects in the prior art are overcome, and the pharmacological activity of the talaromyces purpurascens is enhanced by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, so that the content of Emodic acid in the talaromyces purpurascens is increased.

The invention provides a monascus drying degree real-time control method which comprises the following steps: monitoring the surface moisture content, internal metabolic activity index and pigment content change of mycelium in a monascus drying process, and generating a mycelium state distribution diagram; extracting moisture gradient characteristics and metabolic activity parameters from the mycelium state distribution diagram, analyzing a relationship between a moisture evaporation rate and mycelium activity, and determining a dynamic trend of moisture gradient and activity change; analyzing the dynamic trend of the moisture gradient and the activity change, determining the relationship between the moisture gradient and the activity change through a fitting curve, and predicting the change trend of the moisture and the activity of the mycelium in different drying stages; aiming at a moisture distribution non-uniformity index, the uniform distribution of heat in the red yeast rice is improved by optimizing an air supply mode, so that the moisture evaporation rate deviation is reduced, and a temperature and humidity control sequence is obtained.

The invention provides a preparation method of a cordyceps militaris fermentation product with anti-aging, mitochondrial function enhancing, anti-oxidation and autophagy promoting effects, and the preparation method comprises the following steps: inoculating cordyceps militaris into a fermentation substrate for liquid fermentation culture to obtain a fermentation mycelium; the fermented mycelium is subjected to wall breaking or centrifugal separation, concentration and freeze-drying, and the cordyceps militaris fermented product is prepared; wherein the fermentation substrate comprises oat bran and / or lily. The cordyceps militaris fermentation product prepared by the method shows remarkable anti-aging, anti-oxidation, mitochondrial function enhancing and autophagy promoting effects in in-vitro cell experiments and in-vivo animal models, the process period is short, the preparation process is simple and easy, liquid fermentation in the whole process is suitable for large-scale production, and the preparation cost is low.

The invention provides a culture method for increasing the content of antroquinonol in antrodia camphorata mycelia, and relates to the technical field of medicinal fungus culture. The culture method comprises the steps that antrodia camphorata mycelia are inoculated into a liquid culture medium added with meliaceae cedrela sinensis tender leaf homogenate to be cultured, the liquid culture medium is a PDA culture medium, and the adding amount of the meliaceae cedrela sinensis tender leaf homogenate is 0.5-2 g / 100 mL. According to the method, the defects in the prior art are overcome, the content of antroquinonol in the antrodia camphorata mycelia is increased by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, and the pharmacological activity of the antrodia camphorata mycelia is enhanced.

The invention belongs to the technical field of genetic engineering, and particularly relates to a high-chitin-content engineering bacterium as well as a construction method and application thereof. According to the invention, enzyme related to filamentous fungus chitin synthesis is subjected to overexpression through a genetic engineering means to construct engineering bacteria, the obtained engineering bacteria have high chitin content, and a high-strength mycelium material can be obtained through conventional solid state fermentation. The invention provides a feasible solution with controllable product quality for the production of the high-strength mycelium material, and has good application value.

The invention discloses a tricholoma matsutake culture medium using litchi branches as a matrix and a cultivation method thereof. The tricholoma matsutake culture medium is prepared from the following components in percentage by weight: 30-50% of litchi sawdust, 30-50% of cottonseed hulls, 3-8% of bran, 3-8% of corncobs, 3-8% of rice husks, 1-5% of corn flour and 1-5% of lime. The method is characterized in that the litchi branches are added into the stock liquid culture medium for making the branch seeds for the first time, on one hand, the tricholoma matsutake strains can be trained to generate extracellular enzymes capable of utilizing litchi wood chips, macromolecules of the litchi branches can be rapidly decomposed, and on the other hand, nutrient elements contained in the litchi branches can promote growth of tricholoma matsutake hyphae more rapidly, and the growth rate of the tricholoma matsutake hyphae is increased. The branch seeds are large in inoculation area and high in hybrid resistance, the growth time of mycelium of the cultivation material can be shortened, and the fruiting rate is increased. The invention not only provides a solution for innocent treatment of the litchi branches, but also finds a new culture medium for red tricholoma matsutake culture.

The invention provides inter-plant mycorrhiza nutrient transfer culture equipment which comprises a culture box body and an organic glass plate, an inner cavity of the culture box body is divided into a first culture chamber and a second culture chamber through the organic glass plate, the organic glass plate is a porous glass plate, and a white nylon net is pasted on one side of the porous glass plate. And the white nylon net is divided into a white nylon net with the aperture of 0.45 mu m and a white nylon net with the aperture of 37 mu m. The equipment is small in size, small in occupied area, cheap in material, convenient to assemble and suitable for pot culture experiments of large-batch samples, soil ions are allowed to pass through by means of 0.45 microns, ectogenic hyphae and the soil ions are allowed to pass through by means of a white nylon net of 37 microns, the soil ions, the ectogenic hyphae and root systems are allowed to pass through a nylon-free net, and the soil ions, the ectogenic hyphae and the root systems are allowed to pass through. Compared with the prior art, direct and indirect paths of nutrient transfer between plants can be distinguished and quantified, and the method is simple and easy to implement.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention discloses a hypha network reinforced ecological slope protection structure and a construction method.The hypha network reinforced ecological slope protection structure comprises a plurality of ecological soil nails arranged on a side slope, and each ecological soil nail comprises a soil nail head located outside the side slope; a hollow shell extending into the slope is arranged at one end of the soil nail head, a grouting hole communicated with the hollow shell is formed in the soil nail head, and a plurality of leakage holes are formed in the surface of the hollow shell; a plurality of ecological panels are arranged on the inclined face of the side slope, and the ecological soil nails are filled with grouting liquid with hypha spores. An assembly type structure frame is formed by inserting and matching the ecological panels and the ecological soil nails, grouting liquid with spore bags is injected, after the spore bags are activated, mycelium is combined in a winding mode in the growth process, and a high-ductility biological fiber network structure is constructed between soil particles and components. And the structural integrity and the shear resistance and disturbance resistance of the side slope are improved.

The invention relates to the technical field of agricultural planting, in particular to a Northeast black soil acidification improvement method, which realizes efficient and lasting soil remediation through the synergistic effect of humic acid-calcium complex targeted application, microbial agent activation and green manure planting. According to the technical scheme, the method comprises the steps that soil is partitioned, a first acidification area and a second acidification area are divided according to pH values, humic acid-calcium complexes are applied in a differentiated mode, and pH responsiveness is used for releasing # imgabs 0 # for neutralizing # imgabs 1 # and passivating # imgabs 2 #. The fungus fungicide is applied in furrows, and # imgabs3 is transported to a root system through a hypha network, so that the calcium utilization rate is increased. Soybeans are sown, citric acid is sprayed, mineral calcium is activated through root exudates, and organic matter input is increased. The humic acid-calcium complex is preferentially precipitated in the acidification area. Hypha-root systems cooperatively construct an anti-acidification micro-ecology, so that the yield of crops is increased. The method is suitable for rapid restoration and sustainable management of the severely acidified soil in the northeast black soil area.

The invention belongs to the technical field of mushroom planting, and particularly relates to a pleurotus nebrodensis planting method which comprises the following steps: mixing raw materials of a pleurotus nebrodensis culture medium in parts by weight: 70-85 parts of cottonseed hulls, 5-15 parts of bran and 3-8 parts of corn flour, adding water to enable the water content of the culture medium to be 60%-65%, and uniformly stirring; filling the culture medium into a fungus bag to prepare a cultivation fungus stick; performing high-pressure sterilization on the cultivation mushroom sticks; inoculating the cultivation mushroom sticks in a sterile room; moving the cultivation mushroom sticks into a spawn running room to culture hyphae; after the bags are full of hyphae in the cultivation mushroom sticks, the cultivation mushroom sticks are moved into a growth chamber, buds are thinned after fruiting, and 1-2 mushroom bodies are reserved; after the mushroom bodies are mature, harvesting the mushroom bodies; according to the planting method, the quality and yield of pleurotus nebrodensis can be effectively improved, accumulation of toxins in the culture medium can be reduced, recycling of the culture medium is facilitated, and the production cost of farmer households is reduced.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

A cultivation method for Morchella on patterned layer frames is provided. The method includes: (1) preparing and laying a culture substrate, and sowing; (2) supplementing an exogenous nutrient material; (3) mycelium culture; (4) primordium induction; (5) primordium differentiation; (6) management of a young mushroom period; (7) maturity management; and (8) erchao mushroom management. The method adopts a low temperature of (5-9)° C. to promote a transformation of an exogenous nutrition and adopts a low temperature of (2-4)° C. to induce a differentiation of primordium and other measures, to address a bottleneck of industrial development that cannot continuously supply fresh Morchella to the market, which is caused by some problems in the cultivation of Morchella, such as an unstable yield and seasonal limitation in the existing cultivation of Morchella. The method can carry out industrial facility cultivation for Morchella, and can obtain Morchella with stable yield and good quality.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention belongs to the technical field of fungal fermentation, and particularly discloses ganoderma lucidum fermentation liquor and a preparation method thereof. The preparation method of the ganoderma lucidum fermentation liquor comprises the following steps: (1) activating a strain, namely inoculating the strain into a slant culture medium for culturing, and carrying out transfer grafting once to obtain an activated strain; (2) liquid culture: inoculating the activated strain into a liquid culture medium, and carrying out shake culture for 7 days to obtain a liquid strain; (3) fermentation culture: inoculating the liquid strain into a fermentation culture medium according to the inoculation amount of 7-9%, and carrying out three-stage culture to obtain a fermented product; and (4) centrifugal filtration: centrifuging the fermented product, collecting a supernatant, and filtering the obtained supernatant to obtain the ganoderma lucidum fermentation broth. According to the preparation method disclosed by the invention, three-stage variable-condition culture is specifically carried out according to the growth condition of the ganoderma lucidum mycelia, so that rapid growth of the ganoderma lucidum mycelia and mass accumulation of polysaccharides are facilitated, and the polysaccharide content of the prepared ganoderma lucidum fermentation liquor is increased.

The invention belongs to the technical field of biological pharmacy, and relates to a method for preparing tremella aurantialba polyphenol by utilizing tremella aurantialba TA58.21 fungus chassis cell fermentation, which comprises the following steps: adding a cooked soybean meal culture medium and a tremella aurantialba seed solution into a fermentation tank, introducing sterile oxygen, controlling the stirring rotating speed of the fermentation tank and the culture temperature to be 25-30 DEG C, continuously culturing for 5-8 days, and when the fermentation liquor becomes red, stopping the fermentation; when a large amount of foam is generated, closing an exhaust valve, keeping the tank pressure at 0.1 MPa, increasing the fermentation temperature to 50 DEG C, continuing to culture for 24 hours, increasing the stirring rotating speed of the fermentation tank, heating the fermentation liquor to 80-90 DEG C, keeping the temperature for 4-8 hours, stopping stirring, continuing to heat to 120 DEG C, and keeping the temperature for 30 minutes; centrifuging the fermentation liquor to remove precipitates and mycelia; collecting supernate, and pre-treating macromolecular substances and thallus fragments; collecting permeate liquid, and intercepting through a nanofiltration membrane to obtain a concentrated solution; and carrying out spray drying or freeze drying treatment on the concentrated solution to obtain a high-purity product. According to the invention, the tremella aurantialba polyphenol product with good water solubility, heat resistance, acid resistance and molecular weight of less than 10000 Da can be obtained.

The invention provides a method and system for calculating the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza, and relates to the technical field of calculation of the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza. Soil nitrogen absorption amount data of an experimental group and a control group are collected, meanwhile, the total amount of externally input nitrogen is recorded, and related photosynthetic parameters of plants are measured; in addition, through an in-vitro mycelium culture experiment, mycelium nitrogen absorption rates under different nitrogen concentrations are measured, and a semi-saturation constant and a nitrogen effectiveness index are calculated, so that a carbon-nitrogen coupling factor is calculated, morphological parameters of mycelia are collected, and a relation equation of a pipe diameter and a transport rate is established; calculating the total flux of the hypha network, calculating the original nitrogen utilization rate in combination with the nitrogen absorption amount difference value of the experimental group and the control group and externally input nitrogen data, and introducing a transportation continuity coefficient for dynamic correction to finally obtain the soil nitrogen utilization rate of the target area.

The invention discloses a tricholoma matsutake mushroom, which is classified and named as tricholoma matsutake mushroom 11, and is preserved in China Center for Type Culture Collection on March 24, 2025 with the preservation number of CCTCC NO: M 2025577. The molecular marker identification method comprises culture and collection of hyphae, extraction of genome DNA, DNA fingerprint PCR amplification and electrophoresis detection of PCR products. According to the method, the tricholoma matsutake mushroom 11 which inherits the advantage of high thermotype of two parents, is good in hypha temperature tolerance and high in vigor, is similar to tricholoma matsutake in sporocarp shape and is not opened is obtained by carrying out crossbreeding of the mushrooms and screening after mushroom cultivation and fruiting, and the tricholoma matsutake mushroom 11 has good development and application prospects.

The invention belongs to the technical field of temperature control, and particularly relates to an intelligent temperature control method for an edible mushroom cultivation growth room, which comprises the following steps of: judging temperature abnormity by combining a comprehensive evaluation function of an instantaneous overrun proportion and a standard deviation, and determining a regulation priority according to the temperature abnormity, so that the conversion from average temperature control to abnormal region priority regulation is realized; a shelf two-dimensional temperature distribution diagram is constructed, gradient calculation is carried out, a high-temperature area and the orientation thereof are accurately positioned, the deflection direction and angle of a guide plate are directly controlled through coordinate transformation and nonlinear mapping, cold air flow preferentially flows to the high-temperature area, the problem of uneven horizontal and vertical temperature caused by a three-dimensional cultivation shelf is effectively solved, and the quality of the cultivation shelf is improved. Temperature regulation and control effect verification is intelligently fused with the mycelial growth speed conformity and the fruiting uniformity of the edible mushrooms, the growth conformity is quantified through image analysis, graded early warning is performed based on a secondary verification result, and a complete intelligent closed-loop control system is formed.

The invention discloses Wickerhamomyces anomalus capable of producing volatile compounds and application of the Wickerhamomyces anomalus in prevention and treatment of aspergillus flavus, and belongs to the technical field of microorganisms. According to the invention, a strain of Wickhamomyces anomalus FP246 is obtained through screening, the strain of Wickhamomyces anomalus FP246 is preserved in the China Center for Type Culture Collection on July 31, 2024, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20241719. The Vickerhamomyces anomalus FP246 has an obvious antagonistic effect on aspergillus flavus, and can be used as an effective biological mildew-proof preparation for the dried chilies. The strain can inhibit aspergillus flavus hypha growth and spore germination by generating volatile compounds such as 2-methylbutyric acid, 3-methylbutyric acid and acetic acid. The method for spraying the dried chilies by utilizing the Vickerhamomyces anomalus FP246 is a novel biological control method, and has extremely high research and application values on the development of novel green bacteriostatic drugs.

The present invention relates to a method for obtaining a three-dimensional molded fungal pulp composite, the method comprising the steps of: b) providing a mycelium-colonized substrate pulp slurry; c) molding said slurry into a particular 3-dimensional shape to obtain a molded mycelium-colonized substrate pulp slurry; d) compressing said pulp slurry in order to remove water from the slurry, thereby obtaining the molded mycelium-colonized substrate pulp composite; and e) demolding the so obtained product. The present invention further relates to a blended mycelium substrate slurry, obtainable in step b) of the method of the present invention. The present invention further relates to a molded mycelium-colonized substrate pulp composite obtainable according to the method of the present invention.

The present invention discloses a method for preparing food-grade glassine paper from edible mushroom liquid fermentation mycelium and the food-grade glassine paper, which relates to a method for preparing glassine paper using natural biological materials. The preparation method of the food-grade glassine paper is as follows: Step 1: Preparation of the base paper of edible mushroom liquid fermentation mycelium; Step 2: Preparation of the surface paper of edible mushroom liquid fermentation mycelium; Step 3: Preparation of the food-grade glassine paper. The present invention prepares the food-grade glassine paper from edible mushroom liquid fermentation mycelium, which can replace the traditional papermaking method mainly using wood as the raw material, without adding plastics and chemical coating agents, and has the advantages of environmental protection, low carbon and degradability. It is a food-grade oil-proof, moisture-proof and semi-transparent glassine paper, and has broad application prospects in various fields such as food, medicine and express labels.