708 results about "Mycelium" patented technology

The invention relates to a phellinus igniarius mycelium fermentation product and a preparation method and application thereof, and the preparation method comprises the following steps: inoculating a phellinus igniarius mycelium seed solution into a sterile fermentation culture medium for culturing, carrying out solid-liquid separation, filtering a clear liquid through a membrane and sterilizing to obtain the phellinus igniarius mycelium fermentation product, the fermentation medium comprises the following components: a beet extract, a deep eutectic solvent and water. The beet extract and the deep-eutectic solvent are creatively adopted as culture medium components to prepare the phellinus igniarius mycelium fermentation product, the beet extract and the deep-eutectic solvent jointly provide nutrients needed by phellinus igniarius fermentation, meanwhile, the deep-eutectic solvent is used for regulating metabolism, and compared with a product obtained through fermentation of a traditional culture medium, the phellinus igniarius mycelium fermentation product has the advantages that the fermentation efficiency is high; the content of active ingredients in a fermentation product obtained by adopting the culture medium with the formula is remarkably increased, and the fermentation product is good in uniformity and high in stability, is a clear liquid in appearance, has no abnormal odor and has slight phellinus igniarius characteristic odor.

The invention provides a self-assembled hypha flexible carbon electrode material and a preparation method and application thereof, and belongs to the technical field of battery packs. The preparation method of the self-assembled hypha flexible carbon electrode material comprises the following steps: treating basswood with white rot fungus mycelia, crushing and roasting to obtain porous basswood / hypha composite carbon powder; and putting the porous basswood / hypha composite carbon powder and a carbon nanotube into a sterilized conical flask containing a potato glucose culture solution, inoculating gloeophyllum trabeum, performing activation culture, adding a dopamine hydrochloride aqueous solution, performing polymerization after ultraviolet irradiation, taking out and tabletting to obtain the self-assembled hypha flexible carbon electrode material. In the preparation process of the self-assembled hypha flexible carbon electrode material, the flexible carbon electrode material is synthesized by taking porous basswood / hypha composite carbon powder as a matrix, taking gloeophyllum trabeum hypha as an adhesive, taking polydopamine as a reinforcing agent and adding carbon nanotubes as a conductive reinforcing material, so that the technical problem of poor cycle performance of a lithium ion battery is solved.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention belongs to the technical field of temperature control, and particularly relates to an intelligent temperature control method for an edible mushroom cultivation growth room, which comprises the following steps of: judging temperature abnormity by combining a comprehensive evaluation function of an instantaneous overrun proportion and a standard deviation, and determining a regulation priority according to the temperature abnormity, so that the conversion from average temperature control to abnormal region priority regulation is realized; a shelf two-dimensional temperature distribution diagram is constructed, gradient calculation is carried out, a high-temperature area and the orientation thereof are accurately positioned, the deflection direction and angle of a guide plate are directly controlled through coordinate transformation and nonlinear mapping, cold air flow preferentially flows to the high-temperature area, the problem of uneven horizontal and vertical temperature caused by a three-dimensional cultivation shelf is effectively solved, and the quality of the cultivation shelf is improved. Temperature regulation and control effect verification is intelligently fused with the mycelial growth speed conformity and the fruiting uniformity of the edible mushrooms, the growth conformity is quantified through image analysis, graded early warning is performed based on a secondary verification result, and a complete intelligent closed-loop control system is formed.

The present invention relates to a method for obtaining a three-dimensional molded fungal pulp composite, the method comprising the steps of: b) providing a mycelium-colonized substrate pulp slurry; c) molding said slurry into a particular 3-dimensional shape to obtain a molded mycelium-colonized substrate pulp slurry; d) compressing said pulp slurry in order to remove water from the slurry, thereby obtaining the molded mycelium-colonized substrate pulp composite; and e) demolding the so obtained product. The present invention further relates to a blended mycelium substrate slurry, obtainable in step b) of the method of the present invention. The present invention further relates to a molded mycelium-colonized substrate pulp composite obtainable according to the method of the present invention.

The invention relates to a cordyceps militaris cultivation device which comprises a cultivation frame and a cultivation box, the cultivation frame comprises a plurality of sets of independent cultivation chambers, and each set of cultivation chambers are divided into cultivation cavities of a plurality of levels according to cultivation stages; an inoculated culture medium is contained in the culture box; each group of cultivation chambers is internally provided with an independent transfer mechanism, the transfer mechanisms control the cultivation boxes to gradually transfer from low-grade cultivation cavities to high-grade cultivation cavities according to needs, each cultivation cavity is internally provided with an independent illumination system, and the cultivation cavities of the same grade are internally provided with the same ventilation system; the cultivation boxes in the static state and the corresponding cultivation cavities form closed or sealed spaces; efficient partitioned independent cultivation management is achieved, environment parameters such as illumination, temperature and humidity can be better controlled on the basis of the growth stage of mycelia, high-yield and high-quality cordyceps militaris can be obtained, and the growth processes of the mycelia in different cultivation boxes can be compared and observed more visually.

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

The present invention relates to a method for producing a mycelium sheet. The invention discloses a method for producing a mycelium sheet, which comprises the following steps of: standing and culturing fungi in a liquid culture medium containing cellulose, so that the mycelium sheet is formed on the surface of the liquid culture medium; and collecting the mycelium sheet from the surface of the liquid culture medium containing cellulose.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention relates to the technical field of building materials, in particular to a bio-based multifunctional sound insulation wallboard and a preparation method thereof. The wallboard comprises a sound absorption layer and a sound insulation layer. The sound absorption layer is cooperatively constructed by an inactivated mycelium three-dimensional porous framework and microalgae biomass, and the sound insulation layer is a high-density complex formed by compounding bio-based raw materials such as wood fiber, straw, recycled paper pulp and shell filler. And the two are combined through a biological-physical mechanical interlocking interface formed by hypha growth, extension and embedding to form a natural acoustic gradient structure from porous low acoustic impedance to compact high acoustic impedance. According to the preparation method, an in-situ growth process of mycelium culture and microalgae coating is embedded into a hot-pressing process, a three-dimensional anchoring network is formed by actively embedding mycelia into the sound insulation layer, the microalgae permeates into pores and is subjected to hot pressing to generate biomacromolecules for bonding and filling, a porous structure is synchronously constructed by a one-step method, and firm interlayer bonding is realized. The wallboard has excellent broadband acoustic performance, stable bonding strength and low-carbon and environment-friendly benefits.

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

The utility model discloses a fermentation device of edible fungus mycelia, which relates to the technical field of fermentation devices and comprises a mycelia fermentation tank body, a wall scraping stirring mechanism arranged in the mycelia fermentation tank body and used for stirring materials in the mycelia fermentation tank body, and a filter assembly arranged in the mycelia fermentation tank body and used for filtering the materials in the mycelia fermentation tank body. The wall-scraping stirring mechanism is arranged at the bottom end of the mycelium fermentation tank body and used for filtering a culture solution for fermentation of edible fungus mycelia, the wall-scraping stirring mechanism comprises an output rod, the fermentation device for the edible fungus mycelia is provided with the wall-scraping stirring mechanism, and it can be ensured that materials are fully mixed and fermented in the fermentation process through the wall-scraping stirring mechanism; the material can be effectively prevented from being accumulated on the inner wall of the fermentation tank through the wall scraping assembly, so that the stirring efficiency is improved, the material can be uniformly mixed and fermented, meanwhile, the abrasion of the material to the inner wall of the fermentation tank can be reduced through the wall scraping assembly, and the service life of the equipment is prolonged.

Insects with elevated levels of protein, chitin, elements, amino acids, vitamins, and / or sterols are disclosed. The insects have elevated levels due to a diet that includes mushroom biomass. The mushroom biomass includes any one or more of the mushroom fruitbody, the stem, and the mycelium. The enriched insects with biomolecules from mushroom biomass can be used as they are or as supplements to increase the levels of biomolecules in humans or other animals that may ingest the insects or a modified form of the insect.

In accordance with at least one aspect of this disclosure, a system for filtering storm water runoff comprises, a disposable filter configured to be placed directly in storm water drainage infrastructure for a prescribed filtration period after which the disposable filter is removed from the storm water drainage infrastructure. The disposable filter includes, a permeable container filled with a nutrient rich substrate and a plurality of spores embedded within the nutrient rich substrate configured to develop into mycelium over a prescribed growth period.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses a fermentation material phellinus igniarius cultivation method, and relates to the technical field of phellinus igniarius cultivation.The phellinus igniarius cultivation method specifically comprises the following steps of compost preparation, composting fermentation, strain mixing, bagging spawn running and fruiting management.The invention further provides a culture medium bag for fermentation material phellinus igniarius cultivation. Sealed air bag strips are evenly distributed on the inner side of the packing material, a long-strip-shaped cushion material is arranged between the air bag strips, air holes are evenly distributed in the positions, corresponding to the cushion material, of the packing material, and after the packing material is filled with a fermentation phellinus igniarius culture base material, a fermentation material phellinus igniarius fungus stick is formed. A breathable growth groove used for mycelial growth is formed in the fermentation material yellow mulberry fungus stick; a lower cover body and an upper cover body are fixedly arranged at the two ends of the packing material respectively. The cost is reduced, nutrients are efficiently utilized, the air permeability is good, spawn running is accelerated, hypha activity is enhanced, the pollution rate is reduced, and the quality of sporocarp can be effectively improved.

The invention discloses a preparation method of ganoderma lucidum mutant strain active polysaccharide and application of the ganoderma lucidum mutant strain active polysaccharide in oxidation resistance, and relates to the technical field of biology. The ganoderma lucidum mutant strain active polysaccharide is prepared by taking mycelium of a ganoderma lucidum mutant strain CGMCC No.23283 as a raw material and adopting a water extraction and alcohol precipitation method. The invention develops a novel ganoderma lucidum active polysaccharide, and a ganoderma lucidum strain adopted by the ganoderma lucidum active polysaccharide is cultured by a space mutation technology. Compared with common ganoderma lucidum strain polysaccharide, the polysaccharide provided by the invention is more prominent in antioxidant activity, and is more excellent in key indexes such as total antioxidant capacity and free radical scavenging. The invention provides a new biological material resource for the preparation of antioxidant products.

The application discloses a bucket type cultivation method for overcoming continuous cropping obstacles of bamboo shoots. Through the bucket type inoculation, the mycelium is effectively isolated from the miscellaneous bacteria and pathogenic microorganisms in the soil before the mycelium completes the dominant expansion, the mycelium expansion stability is obviously improved, and the mycelium death and yield loss caused by the continuous cropping obstacles are avoided. Field tests show that the yield of the second year can be recovered to more than 90% of the yield of the first year by using the method, and the yield of the second year is less than 10% of the yield of the traditional reseeding mode, and almost no yield is obtained, so the method has good stability and generalizability.

A method for producing mycelium biomass. The method comprises obtaining a base media composition and an antifoam solution; sterilizing a mixture of the base media composition and the antifoam solution

The present invention relates to the production of biocomposite materials through the co-cultivation of mycelium and algae. This innovative method integrates sustainable practices and utilizes waste materials to create carbon-negative, durable, and biodegradable composites suitable for various applications, including interior design, construction, automotive and packaging.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The present invention relates methods for manufacturing a biodegradable and eco-friendly mulching agent for soil and flowerpots, a biodegradable coffin, and a biodegradable cinerary urn, the methods using mushroom mycelium growth, and a biodegradable and eco-friendly mulching agent, a biodegradable coffin, and a biodegradable cinerary urn manufactured thereby. The biodegradable and eco-friendly mulching agent for soil and flowerpots is completely biodegradable and exhibits excellent viscosity, thus being highly suitable for being molded into and used as a mulching agent for soil and flowerpots, and can reduce post-treatment costs and steps and mitigate environmental pollution. The biodegradable and eco-friendly coffin and cinerary urn is biodegradable and exhibits excellent physical properties such as strength and air permeability, thus being suitable for use in forms of burial such as tree burials or pot burials for companion animals.

The application discloses application of a fungal virus in prevention and treatment of pear tree and apple tree Valsa diseases. The fungal virus is a positive single-stranded RNA virus, and a cDNA nucleotide sequence of the fungal virus is shown as SEQ ID No. 1. A fungus containing the virus is Valsa pyri HJL1-22 (gamma), the fungus is infected by the fungal virus of claim 1, the fungus is named as HJL1-22 (gamma), is classified and named as Valsa pyri, and has a preservation number of CGMCC No. 41449 in China General Microbiological Culture Collection Center. The virus Vphv1-gamma can be secreted to outside of cells, therefore, culture liquid filtered off mycelium can be used as a biocontrol agent for prevention and treatment of pear tree and apple tree Valsa diseases, and can effectively weaken or inhibit pathogenicity and diffusion of Valsa pyri and Valsa mali, and has a prospect for being used for producing and practicing biological prevention and treatment of pear tree and apple tree Valsa diseases.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention relates to methods of cultivating ectomycorrhizal fungi, the method comprising incubating ectomycorrhizal MAT1-1 mycelia, ectomycorrhizal MAT1-2 mycelia, and ectomycorrhizal male mycelia in a culture medium for about 1 day to about 50 days, thereby cultivating ectomycorrhizal fungi to produce an ectomycorrhizal fungal biomass comprising MAT1-1 ectomycorrhizal mycelia, MAT1-2 ectomycorrhizal mycelia, and male ectomycorrhizal mycelia. Also provided are methods of use of the ectomycorrhizal mycelial biomass.

The invention relates to the technical field of traditional Chinese medicinal material planting. The invention provides a container confinement cultivation method for relieving asexual propagation continuous cropping obstacles of gastrodia elata, which comprises the following steps: (1) filling and paving planting soil in a container, placing wood sections and armillaria mellea, then earthing, carrying out heat preservation treatment, and culturing to obtain a fungus bed; and (2) exposing the fungus material, placing gastrodia elata seeds around the fungus material, covering soil, carrying out heat preservation treatment, and carrying out conventional planting management. By means of the cultivation method, centralized and standardized cultivation of the gastrodia elata can be promoted, the problem that suitable cultivation sites are gradually reduced in the gastrodia elata cultivation process is solved, the problem of continuous cropping obstacles of the gastrodia elata is effectively solved, and sustainable development of the gastrodia elata is promoted.

The invention belongs to the field of medicine, and discloses a composition containing hirsutella hepialid mycelium and lucid ganoderma, a preparation method and application of the composition in preparation of a medicine for relieving cough and eliminating phlegm. The composition containing the hirsutella hepialid mycelium and the lucid ganoderma is prepared from a hirsutella hepialid mycelium extract and a lucid ganoderma extract. The composition containing the hirsutella hepialid mycelium and the lucid ganoderma can be used for preparing health care products or medicines for relieving cough and eliminating phlegm. The traditional Chinese medicine composition can be prepared into tablets, capsules, granules, oral liquid and other dosage forms. The ganoderma lucidum extract and the hirsutella hepialid hypha extract are combined, the synergistic effect of the ganoderma lucidum extract and the hirsutella hepialid hypha extract is exerted, and the effects of relieving cough and eliminating phlegm are remarkably enhanced.