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39 results about "Submerged fermentation" patented technology

Submerged fermentation. Submerged fermentation is a method of manufacturing biomolecules in which enzymes and other reactive compounds are submerged in a liquid such as alcohol, oil or a nutrient broth.

High-activity hericium erinaceus mycelium polysaccharide and preparation method thereof

The invention discloses a method for efficiently preparing high-activity hericium erinaceus polysaccharide from waste in the edible mushroom industry, and belongs to the technical field of deep processing of edible mushrooms and recycling of waste. The method comprises the following steps: carrying out steam explosion pretreatment on hericium erinaceus waste mushroom residues, and carrying out synergistic compatibility on the hericium erinaceus waste mushroom residues, tea residues, bean residues and citrus residues which are subjected to specific enzymolysis to prepare a liquid fermentation culture medium; a two-stage control strategy is adopted for submerged fermentation of hericium erinaceus liquid, and hypha proliferation and polysaccharide synthesis conditions are optimized; and after fermentation is finished, extracting and purifying mycelium polysaccharide by adopting a choline chloride-lactic acid eutectic solvent system. According to the method, high-valued utilization of the edible fungus chaff and various agricultural byproducts is achieved, the content of beta-(1-> 3)-D-glucan in the prepared hericium erinaceus polysaccharide is larger than or equal to 55%, the immunocompetence (IC50) reaches 32 micrograms / mL, the hericium erinaceus polysaccharide is remarkably superior to commercially available products, and the hericium erinaceus polysaccharide can be directly used as functional food and medicine raw materials. The process is green and efficient, and has remarkable economic and environmental benefits.
Owner:INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI

Natural plant effect micromolecule fermentation process display equipment for skin care products

The utility model discloses natural plant effect micromolecule fermentation process display equipment for skin care products, which comprises a display cabinet, and a controller, a transparent material box and at least one process demonstration device are arranged in the display cabinet. The process demonstration device comprises a display screen, a first inductor, a first feeding pipe, a first material returning assembly, a plurality of first demonstration balls and a first driving mechanism, the two ends of the first feeding pipe are communicated with the material box and the first material returning assembly respectively, and the first inductor is arranged at the end, close to the display screen, of the first feeding pipe; when the first sensor senses the first demonstration ball, the controller controls the first driving mechanism to drive the first demonstration ball to return to the material box and controls the display screen to play a pre-stored video. Natural plant effect micromolecules are displayed to people through an electromechanical and multimedia combined closed system, and the process of enriching active substances and achieving the effect of enhancing the effect is achieved through submerged fermentation of microorganisms, so that people know and are familiar with the concept of plant skin care.
Owner:GUANGDONG SCI CENT

Recombinant cordyceps militaris strain with high yield of beta-glucan as well as construction method and application of recombinant cordyceps militaris strain

The invention provides a recombinant cordyceps militaris strain with high yield of beta-glucan as well as a construction method and application of the recombinant cordyceps militaris strain, and belongs to the technical field of bioengineering and genetic breeding of edible fungi. According to the invention, a cordyceps militaris CmGls super-safe overexpression vector is constructed through a CRISPR-Cas9 technology, and then the cordyceps militaris CmGls super-safe overexpression vector is transferred into cordyceps militaris to obtain a recombinant cordyceps militaris strain with high yield of beta-glucan. According to the recombinant cordyceps militaris strain, membrane binding type beta-1, 3-glucan synthetase gene CmGls is accurately inserted into a safe port site CmSh1 of a cordyceps militaris genome, compared with a wild type cordyceps militaris strain, the growth rate of the recombinant cordyceps militaris strain is remarkably increased by 15% or above, and yellow phenotype and cell wall stress resistance are remarkably enhanced; after 6 days of submerged fermentation, the mycelium amount and the extracellular beta-glucan yield are respectively improved by more than 10%, and the strain has good application in improving beta-glucan synthesis.
Owner:JIANGSU UNIV

Straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement and preparation method of straw-based biochar composite slow-release fertilizer

The invention belongs to the technical field of organic fertilizers, and particularly relates to a straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement and a preparation method of the straw-based biochar composite slow-release fertilizer. The preparation method comprises the following steps: (1) uniformly mixing sheep manure and pigeon manure, mixing the mixed manure with crushed corn straw to obtain a total material, inoculating bacillus subtilis, and composting and fermenting to obtain a primary organic matter; (2) fermenting the primary organic matter by using aspergillus niger, trichoderma reesei and saccharomyces cerevisiae to obtain a submerged fermentation organic matter; (3) crushing the submerged fermentation organic matter to prepare particles; the composite coating powder is scattered into the submerged fermentation organic matter, coating is conducted, and the coated organic matter is obtained; and (4) uniformly mixing the coated fermented organic matter, biochar, desulfurized gypsum, humus soil, a water-retaining agent and ammonium dihydrogen phosphate to obtain the straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement. The organic fertilizer can improve the pH value of saline-alkali soil, reduce the salt content and increase the organic matter content.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI

Novel high-oxidation-resistance fungus and grain ingredient powder produced from ganoderma lucidum fermented whole wheat as well as preparation method and application of novel high-oxidation-resistance fungus and grain ingredient powder

The invention relates to novel high-oxidation-resistance fungus and grain ingredient powder produced through ganoderma lucidum fermented whole wheat and a preparation method and application of the novel high-oxidation-resistance fungus and grain ingredient powder, and belongs to the field of food processing.The method comprises the steps that ganoderma lucidum strains are subjected to liquid submerged fermentation and hypha crushing culture, and high-activity liquid strains are obtained; performing soaking and cooking pretreatment on the whole wheat; mixing the pretreated whole wheat with nutrient substances, sterilizing, inoculating the liquid strain, and fermenting in a special breathable bag; and drying and grinding the fermentation product to obtain the fungus grain ingredient powder. Lucid ganoderma is utilized to efficiently convert combined polyphenols with low bioavailability in whole wheat into free polyphenols, and active ingredients of the lucid ganoderma are cooperated, so that the total phenol content and the antioxidant activity of the product are remarkably improved. The mushroom grain ingredient powder can be applied to whole-grain foods such as steamed buns, noodles and bread, and has the characteristics of natural products, nutrient enrichment and the like.
Owner:INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI

Process for producing plant-based fermented food or feed with umami-rich sensory profile, reduced off flavor and multifunction, products or ingredients obtained therefrom and uses thereof

The present invention relates to a process for producing plant-based fermented food or feed containing plant protein with umami-rich sensory profile and improved nutritional profile, as well as products obtained therefrom and uses thereof. In particular the present invention relates to a process for producing fermented food or feed wherein the plant proteins are cross-fermented by the use of the combination of Propionibacterium spp., Lactobacillus spp., Aspergillus spp., and Saccharomyces spp. as well as products obtained therefrom and uses thereof. The process of the invention is based on the use of solid-state-fermentation (SSF) and submerged fermentation (SMF).
Owner:NUTRUMAMI APS

Method for preventing and treating rice blast by using compound microorganism fermentation liquor

The invention discloses a method for preventing and treating rice blast by using compound microorganism fermentation liquor, and relates to the technical field of agricultural biologication.The method comprises the following steps that S1, bacillus strains with antagonistic activity on rice blast bacteria are selected for compound culture; the method comprises the following steps: respectively carrying out activation culture on selected bacillus of different species and strains to obtain respective seed solutions; and mixing the seed solutions according to a predetermined proportion, performing liquid submerged fermentation culture under controllable conditions, and controlling fermentation parameters to proliferate the compound microorganism thalli to a predetermined high concentration. According to the method for preventing and treating the rice blast through the compound microorganism fermentation liquor, the compound microorganism fermentation liquor preparation which can be directly used for spraying is prepared through compound culture of bacillus strains with antagonistic activity, so that the problems of drug resistance, pesticide residues and environmental pollution caused by chemical pesticides are effectively solved; the characteristics of greenness and environmental protection are shown, and the control effect on the rice blast is improved.
Owner:ANREN COUNTY SHENGPING RICE IND +1

Improved molasses culture medium and application method thereof in increasing yield of bird nest alkane diterpenoid high-activity compounds

The invention discloses an improved molasses culture medium and an application method of the improved molasses culture medium in increasing the yield of bird nest alkane diterpenoid high-activity compounds, and relates to the technical field of microbial fermentation. The invention aims to solve the problems of long production period, low product yield, large batch difference and difficulty in large-scale and process control when a solid culture medium is used for static fermentation in the existing production method. The method comprises the following steps: inoculating 0.1-0.5% of black egg nest fungus into a flat plate solid culture medium for culture, and then transferring into a liquid seed culture medium for shake culture to obtain a seed solution; inoculating the seed liquid into a fermentation tank filled with an improved molasses culture medium for deep fermentation to obtain fermentation liquid; mycelia are separated from the fermentation liquor, and a product rich in the bird nest alkane diterpenoids is obtained through washing, quick freezing, drying, smashing, extracting and concentrating. The invention can obtain the improved molasses culture medium and the application method of the molasses culture medium in increasing the yield of the bird's nest alkane diterpenoid high-activity compounds.
Owner:NORTHWEST A & F UNIV

Preparation method for synergistically producing biological agent from bacillus and lactobacillus plantarum, biological agent and application

The invention relates to a preparation method of a biological agent synergistically produced by bacillus and lactobacillus plantarum, the biological agent and application, and relates to the technical field of bacteriocin preparation. The liquid culture medium contains yeast extract powder and glucose obtained by performing differential temperature asynchronous enzymolysis on starch slurry; s2, uniformly spraying the submerged fermentation liquid obtained in the step S1 on the surface layer of a solid fermentation culture medium within the thickness of 0.5-1 mm for primary fermentation, inoculating lactobacillus plantarum FJS003 into the surface layer of the solid fermentation culture medium within the thickness of 2-3 mm for secondary fermentation after the primary fermentation is finished, and obtaining the production biological agent after the secondary fermentation is finished. The prepared biological agent can effectively inhibit the growth speed of escherichia coli, salmonella and staphylococcus aureus, and meanwhile can effectively degrade aflatoxin B1 and the content of lead, arsenic, mercury and cadmium.
Owner:HANBOK KITCHEN ELECTRIC APPLIANCE TECH

Special culture medium for culturing trichoderma reesei and producing cellulase for preparing lignocellulose biomass hydrolysis sugar and application of special culture medium

PendingCN120484974AFungiMicroorganism based processesSubmerged fermentationEngineering
The invention discloses a special culture medium for culturing trichoderma reesei and producing cellulase for preparing hydrolysis sugar of lignocellulose biomass (represented by corncobs or straws and the like) and application of the special culture medium. According to the culture medium, a mixture of industrial glucose with low price and a cereal processing byproduct bran with lower price is used for replacing microcrystalline cellulose and lactose or whey powder which are commonly used at present and are high in price, so that the material consumption cost of cellulase liquid submerged fermentation production is remarkably reduced; the production cost of the cellulase is reduced to the technical and economic indexes required by hydrolysis of (semi) cellulose components in lignocellulose biomass represented by corncobs or straws and the like to prepare hydrolyzed sugar, and the special requirements of fermentation production of cellulosic ethanol, other bulk bio-based products and the like are met.
Owner:SHANGHAI JIAOTONG UNIV

Brevibacillus parvus GBW-M0 and application thereof in aquaculture

The application provides a Brevibacillus parabrevis GBW-M0 and application thereof in aquaculture. The Brevibacillus parabrevis GBW-M0 is classified as Brevibacillus parabrevis Brevibacillus laterosporus , and the preservation number is CGMCC No. 32335. The Brevibacillus parabrevis GBW-M0 is prepared through seed liquid preparation and submerged fermentation to obtain Brevibacillus parabrevis GBW-M0 fermentation liquor. The Brevibacillus parabrevis GBW-M0 fermentation liquor can reduce the concentration of hepatointestinal ciliates carried in the Penaeus vannamei, and is used for preventing and controlling microsporidian diseases of aquaculture animals. Meanwhile, the Brevibacillus parabrevis GBW-M0 fermentation liquor can inhibit the growth of Microcystis aeruginosa, and is used for treating blue-green algae blooms in aquaculture water bodies, thereby improving the success rate of aquaculture.
Owner:QINGDAO SHANGDE BIOTECH +1

Camellia oleifera shell mycelium composite material and application thereof

PendingCN120966265AFungiFlexible coversCamellia oleiferaSubmerged fermentation
The invention belongs to the field of agricultural and forestry waste resourceful treatment, and discloses a camellia oleifera shell mycelium composite material and application thereof. The preparation method comprises the following steps: inoculating white rot fungus liquid seeds into a camellia oleifera shell solid-state fermentation substrate, uniformly mixing, filling into a mold, carrying out first-time solid-state fermentation, demolding, carrying out second-time solid-state fermentation until hyphae grow all over the solid-state fermentation substrate, and drying and shaping until a compact mycelium film is formed on the surface, so as to obtain the camellia oleifera shell mycelium composite material. According to the preparation method combining liquid submerged fermentation and solid state fermentation, the production efficiency of the mycelium composite material is remarkably improved, and the prepared composite material is hydrophobic in surface, good in compression resistance, rebound rate, heat insulation performance and flame retardant performance, wide in application range and capable of being used for preparing various heat preservation and insulation packaging and building materials.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

High-efficiency liquid submerged fermentation culture medium of russula vinosa polysaccharide and fermentation method

The invention relates to an efficient liquid submerged fermentation culture medium of russula vinosa polysaccharide and a fermentation method, and belongs to the technical field of fermentation. The formula of the efficient liquid submerged fermentation culture medium for russula vinosa polysaccharide comprises the following components: glucose, a russula vinosa stem water extract, a schizochytrium limacinum degreased powder water extract, a nannochloropsis oculata degreased powder protein enzymatic hydrolysate, an anoectochilus formosanus leaf water extract, a soybean cake powder protein enzymatic hydrolysate, wort, proteoglycan, monopotassium phosphate and magnesium sulfate heptahydrate. On the basis of the fermentation culture medium, a gradient fermentation technology is matched, efficient fermentation production of russula vinosa polysaccharide is achieved by adjusting the components and nutrient concentration of the culture medium in stages, the bottleneck of current russula vinosa polysaccharide resource acquisition is broken through, and industrial application of russula vinosa polysaccharide is promoted.
Owner:INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI

A method for improving the yield of arachidonic acid produced by fermentation of mortierella

This invention discloses a method for increasing the yield of arachidonic acid (RAA) produced by *Morchella spp.* fermentation, relating to microbial fermentation technology. The method employs either solid-state fermentation or liquid-state shallow static fermentation. *Morchella spp.*, used for RAA synthesis, is inoculated onto the surface of a solid-state fermentation medium for solid-state fermentation, or inoculated into a liquid fermentation medium for liquid-state shallow static fermentation. Feeding is performed during fermentation until the RAA content in the *Morchella spp.* reaches its peak. This method can increase the RAA content in synthesized oils to 1.72 times that of deep fermentation, reaching a maximum of 80.93%. The yield of RAA synthesized by *Morchella spp.* per 1L of culture medium consumed is up to four times that of deep fermentation. This method requires less equipment investment, is simple to operate, has very low energy consumption, is pollution-free, and allows for large-scale production of RA-rich oils and microorganisms, showing broad application prospects in pharmaceutical intermediates, nutritional supplements, and animal feed.
Owner:HUAZHONG UNIV OF SCI & TECH

Systems and Methods of Liquid Submerged Fermentation Using Liquid Dilution Spawning for Expanding Inoculum of Fungal Species

PendingUS20250327017A1Bioreactor/fermenter combinationsFungiBiotechnologySubmerged fermentation
Embodiments relate to systems and methods for liquid submerged fermentation to expand inoculum of fungal species, addressing the technical problem of lengthy production times in fungal culture. The solution involves a method comprising inoculating growth media in a sterilized container, agitating the media under optimized conditions, diluting the fungal species in a secondary container, and inoculating bioreactor bags to increase biomass volume. This process enables even colonization and consolidation of the fungal species, resulting in full spectrum fungal biomass production. Embodiments include efficient production of fungal biomass with reduced lead time and labor costs compared to standard systems. Embodiments are applicable to various fungal genera, utilizing specific growth media compositions and controlled environmental conditions to optimize growth and production.
Owner:M2 INGREDIENTS INC

Preparation method of bacteriostatic and growth-promoting functional microbial fertilizer

The invention discloses a preparation method of a bacteriostatic and growth-promoting functional microbial fertilizer, and particularly relates to the field of agricultural biotechnology and microbial fertilizers, and the preparation method comprises the following specific preparation steps: functional strain screening, seed liquid preparation, liquid submerged fermentation, organic fertilizer matrix pretreatment, compound fermentation and post-treatment. According to the preparation method, the fermentation liquor with high activity and high concentration is obtained through an optimized liquid fermentation process, and then the fermentation liquor and the pretreated organic fertilizer matrix are subjected to compound fermentation, so that the multifunctional microbial fertilizer which is high in viable count, good in stability and capable of effectively preventing and treating soil-borne diseases and remarkably promoting crop growth at the same time is finally prepared. The use amount of a chemical fertilizer is greatly reduced; under the condition that the use amount of the chemical fertilizer is reduced by 30%, the crop yield can still be kept and even increased by 5%-10%, the investment of chemical pesticides and chemical fertilizers can be reduced by applying the fertilizer, non-point source pollution is reduced, and the soil micro-ecology is improved.
Owner:WEIFANG UNIVERSITY

Degradable flexible conductive mycelium material and preparation method thereof

The application discloses a kind of degradable flexible conductive mycelium material method, belong to new material technical field.The application utilizes deep fermentation to prepare mycelium nanoparticle composite fermentation liquor, and is combined with solid fermentation, and the resistance value of the conductive mycelium material prepared is 30-40 Ω / cm, with excellent conductive performance, and after repeatedly stretching 100 times, resistance value fluctuation is about 10%, and the stability of guide point is excellent;In addition, degradation rate reaches about 70%, and can be recycled, compared with traditional conductive material, it will not produce a large number of electronic garbage, and reduce the dependence on earth resources, will not cause harm to the environment.Meanwhile, the breaking strength of the flexible conductive mycelium material prepared by the application reaches more than 25MPa, and the contact angle is >90°.Therefore, the conductive mycelium material prepared by the application is similar to leather in appearance, and the conductivity and flexibility are good, which can promote the development of intelligent textiles.
Owner:JIANGNAN UNIV

Enzymolysis extraction method for morchella esculenta through liquid submerged fermentation, extract and application

The invention discloses a liquid submerged fermentation morchella enzymolysis extraction method, an extract and application, and belongs to the technical field of morchella enzymolysis, and the method comprises the following steps: separating and purifying morchella; activating the strain; liquid fermentation culture: inoculating the seed liquid into a fermentation culture medium; centrifugally separating the fermentation liquor, collecting morchella mycelia, washing with distilled water, and drying for later use; enzymolysis, wherein the mass ratio of cellulase to pectinase to flavourzyme to alkaline protease to neutral protease in a compound enzyme is (2.8-3.1): (1.7-2.2): (0.4-0.6): (0.1-0.3): (0.2-0.4); centrifuging and collecting supernate; concentrating the supernate to obtain a morchella enzymatic hydrolysate concentrate; and drying and crushing the morchella esculenta enzymatic hydrolysate concentrate. According to the method, the yield of the extract, the content of polysaccharide and protein in the extract and the antioxidant activity of the extract can be effectively increased.
Owner:YAOSYNBIO BIOTECHNOLOGY (NANJING) CO LTD

Method for fermenting, debitterizing and removing cold nature of rabdosia serra

The invention relates to the technical field of rabdosia lophanthide debitterizing and cold dispelling, and discloses a rabdosia lophanthide fermentation debitterizing and cold dispelling method which comprises the following steps: pretreatment: chopping rabdosia lophanthide by a chopper to obtain rabdosia lophanthide of which the particle size is less than or equal to 5mm, and putting the chopped rabdosia lophanthide into a steam enzyme deactivation instrument for enzyme deactivation treatment; performing liquid submerged fermentation: putting the rabdosia serra subjected to fixation treatment into a liquid fermentation tank, inoculating lactobacillus plantarum and aspergillus niger into the liquid fermentation tank at the same time, and performing submerged fermentation for 48 hours; solid-state secondary fermentation: placing a liquid product after liquid-state submerged fermentation in a solid-state fermentation box, planting saccharomyces cerevisiae boulardii in the solid-state fermentation box, and carrying out solid-state secondary fermentation for 72 hours; drying: carrying out drying treatment on the semi-finished product subjected to solid secondary fermentation; the rabdosia lophanthide with the bitter taste removed and the cold property removed is obtained. Stepped degradation of bitter substances and conversion of cold structures are realized; the problem of bitter cold is solved through biotransformation, and people with liver diseases / weakness of spleen and stomach are adapted.
Owner:GUANGZHOU LOHAS BIOLOGICAL TECH

A strain of Tremella fuciformis and its fermentation method for producing Tremella fuciformis polysaccharide and its application

PendingCN122278642ABiotechnologyMicroorganism
This invention discloses a *Tremella fuciformis* strain and its method and application for fermenting to produce *Tremella fuciformis* polysaccharide, belonging to the field of microbial fermentation technology. The *Tremella fuciformis* strain has the preservation number CGMCC No. 42524. This invention utilizes this strain for liquid submerged fermentation to produce *Tremella fuciformis* polysaccharide. Through synergistic optimization of the culture medium composition, the method of adding mycelial extract, and fermentation control parameters, a highly efficient fermentation system suitable for this strain was constructed. Results show that adding 10% mycelial extract to the basic fermentation medium can significantly increase the yield of *Tremella fuciformis* polysaccharide. This strain still exhibits good scale-up adaptability in a 200 L fermenter, with a mycelial dry weight of 65.03±0.25 g / L, a polysaccharide content of 50.88±0.11%, and a polysaccharide yield of 33.09±0.20 g / L. This invention provides a new strain resource and technical solution for the efficient, stable, and large-scale industrial production of *Tremella fuciformis* polysaccharide.
Owner:JILIN JISHENG PHARMA

Application of grifola frondosa ferment in reducing methane emission of dairy cows

The application provides application of a grifola ferroxida ferment in preparation of a feed additive for reducing methane emission of a dairy cow. The grifola ferroxida ferment is obtained by liquid submerged fermentation or liquid-solid two-phase fermentation with grifola ferroxida as an object. After the grifola ferroxida ferment is fed to the dairy cow, the activity of a fiber-decomposing bacterial group can be enhanced, the feed digestion rate is improved, the abundance of some bacteria in a rumen is improved, the activity composition of a rumen microbial population is changed, and the methane emission of the dairy cow is reduced.
Owner:HEFEI MAI KELUO BIOLOGICAL ENG CO LTD

Submerged fermentation tank

A submerged fermentation tank comprises a tank body, and an opening is formed in the upper end of the tank body. The multiple containing pieces are arranged in the tank body in the vertical direction, vegetables are placed in the containing pieces, the problem that the vegetables float in the fermentation process is avoided, after the vegetables are fermented, the vegetables can be conveniently lifted out of the tank body through a travelling crane and the like, and therefore the vegetables can be conveniently fished out in the fermentation process, and the fermented vegetables can be rapidly transferred and packaged. A plurality of detachably-arranged limiting mechanisms are arranged in the tank body, a plurality of clamping pieces are arranged on the limiting mechanisms in the vertical direction, the clamping pieces are used for the upper ends of the containing pieces to limit the containing pieces, and when fermentation water is injected into the tank body, the containing pieces and vegetables move upwards due to buoyancy and the like, so that the vegetables are prevented from entering the tank body. And by arranging the clamping piece, the containing piece can be limited, so that the containing piece is prevented from floating. In addition, in order to conveniently take out the containing piece, the limiting mechanism is detachably arranged on the tank body.
Owner:SICHUAN ACAD OF FOOD & FERMENTATION INDS

Application of Acremonium truncatum F2652 in the preparation of preparations for improving production performance, immunity or antioxidant of weaned piglets

The present invention relates to the application of Acremonium terrestris F2652 in the preparation of preparations for improving the production performance, immunity or antioxidant of weaned piglets, and belongs to the field of strain fermentation technology. The contents of active ingredients such as cordycepin and polysaccharides in the secondary metabolites of Acremonium terrestris F2652 of the present invention are significantly improved, thereby achieving the goal of increasing the content of cordycepin, a characteristic component of Cordyceps fungi, while also achieving the conversion and enrichment of polysaccharides of the Acremonium terrestris strain. The fermentation culture obtained by liquid submerged fermentation of Acremonium terrestris F2652 combines the advantages of both parents, retains the ability to synthesize excellent complex polysaccharides, and integrates good cordycepin accumulation potential, and has the ability to develop Acremonium terrestris cultures with high cordycepin content, which is conducive to enriching its practical application as a functional feed additive in different animals.
Owner:GUANGXI UNIV +2

Preparation method of monascus red pigment with low citrinin

The invention relates to the field of food additive pigment extraction, in particular to a preparation method of monascus red pigment with low citrinin. According to the method for performing liquid submerged fermentation by adopting a monascus strain, vegetable oil is added into a culture medium, production of citrinin in the fermentation process is controlled in a mode of feeding hydrogen peroxide in the fermentation process, and monascus red pigment is extracted; the monascus purpureus is subjected to liquid culture, acid precipitation, filtration, washing and filtration, a filter cake is collected, and the filter cake is extracted and refined with food-grade ethanol. The content of citrinin in the monascus red pigment obtained by using the method is extremely low, the quality and safety of a monascus product are greatly improved, and the method has relatively high industrial application value and is beneficial to popularization and application of monascus red.
Owner:HENAN ZHONGDA HENGYUAN BIOTECH CO LTD

Cycling fermentation, process and system for submerged fermentation

Fermentation processes are described herein. The processes include a) supplying a first fermentation tank with a fermentation broth; b) supplying gas from an external gas source to the first fermentation tank and to a second fermentation tank; c) during step b), venting gas from the second fermentation tank into the first fermentation tank via a fluid pathway, and venting gas from the first fermentation tank via a vent of the first fermentation tank; and d) during step b) and after step c), closing the vent of the first fermentation tank, and opening a vent of the second fermentation tank, to force the fermentation broth from the first fermentation tank into the second fermentation tank via the fluid pathway.
Owner:DEAN PETER

Method for obtaining a lyophilised enzymatic preparation and its uses in synthesis reactions for the production of estolide-type biolubricants, biodiesel and polyol-esters-type biolubricants

The present disclosure describes a method for obtaining a proprietary biocatalyst, called lyophilized enzyme preparation (PEL) via submerged fermentation (SmF) of Diutina rugosa yeast. The biocatalyst obtained by the process is also applied in synthesis reactions for the production of estolide-type biolubricants; biodiesel and polyol-esters-type biolubricants.
Owner:PETROLEO BRASILEIRO SA PETROBRAS +1

Application of liquid culture medium for fermentation of phlebopus portentosus liquid strain

PendingCN120944718AFungiMicroorganism based processesBiotechnologyPhlebopus portentosus
The invention relates to application of a liquid culture medium for fermentation of a phlebopus portentosus liquid strain, and belongs to the technical field of edible fungus strain fermentation culture media. The liquid culture medium comprises the following components in parts by weight: 10-30 parts of molasses, 10-30 parts of soluble starch, 2-5 parts of yeast extract, 1-4 parts of monopotassium phosphate, 1-3 parts of magnesium sulfate, 0.1-1 part of adenine, 0.3-0.5 part of a defoaming agent and 1000 parts of water, and the pH value of the liquid culture medium is 5.0-5.8. Hypha blocks of phlebopus portentosus are inoculated and cultured to obtain mother strains, then the mother strains are inoculated into the liquid culture medium to be fermented to obtain seed liquid, the seed liquid is transferred into the liquid culture medium in a fermentation tank to be deeply fermented, and the liquid strains are obtained. The liquid culture medium can be fermented in a short time to obtain a large number of phlebopus portentosus liquid strains, the submerged fermentation time of the phlebopus portentosus liquid strains is greatly shortened, the quality of the liquid strains is excellent, and a new way is provided for industrial application of the phlebopus portentosus liquid strains.
Owner:YUNNAN JUNSHIJIE BIOTECHNOLOGY CO LTD

Solid state fermentation optimization method of GA4+7 mutant strain

PendingCN120966642AFungiOrganic chemistryBiotechnologySubmerged fermentation
The invention provides a solid state fermentation optimization method of a GA4 + 7 mutant strain. According to the method, a solid-state fermentation culture medium and solid-state fermentation conditions for producing gibberellin GA4 + 7 are explored and optimized, bran is selected as a solid-state culture medium structure support, meanwhile, the initial water content of the solid culture medium is optimized, the optimal water content is determined to be 60%, the yield of gibberellin GA4 + 7 is increased, the yield of gibberellin GA4 + 7 is increased, and the yield of gibberellin GA4 + 7 is increased. The gibberellin can be conveniently extracted after fermentation is finished. In the extraction stage, 20% methanol is added in the extraction stage, so that the extraction amount of gibberellin can be increased, and the use amount of an organic solvent is also reduced. Finally, under the cooperation of various factors such as a structural support, the initial water content of a solid culture medium and a solid-state fermentation product extraction method, after solid-state fermentation and extraction are carried out on the gibberellic gibberellic mutant strain, the yield of the GA4 + 7 reaches 6.22 g / kg, and the liquid submerged fermentation level is greatly improved.
Owner:ZHEJIANG UNIV OF TECH

Fungal ingredients and derived products

The present invention relates to mycelium ingredients derived from submerged fermentation of at least one fungal strain in three different mediums: a defined medium leading to ingredient A, a synthetic medium leading to ingredient B, or a complex natural medium comprising a sidestream extract originated from an agrifood sidestream leading to ingredient C. Such unique mycelium compositions are characterized chemically, biologically, physically, morphologically, nutritionally, and organoleptically. The three different fibrous mycelium mass of edible fungi obtained from at least one fungal strain are further used to produce characterized food products, including meat analogues, fish analogues, dairy analogues, beverages, or other food products. These three new mycelium ingredients can be used in the manufacturing of foods, foodstuffs, beverages, pharmaceutical, cosmetics, nutraceutical, biomaterials, and feed and industrial applications.
Owner:MUSHLABS GMBH