19 results about "Submerged fermentation" patented technology

The invention discloses a method for efficiently preparing high-activity hericium erinaceus polysaccharide from waste in the edible mushroom industry, and belongs to the technical field of deep processing of edible mushrooms and recycling of waste. The method comprises the following steps: carrying out steam explosion pretreatment on hericium erinaceus waste mushroom residues, and carrying out synergistic compatibility on the hericium erinaceus waste mushroom residues, tea residues, bean residues and citrus residues which are subjected to specific enzymolysis to prepare a liquid fermentation culture medium; a two-stage control strategy is adopted for submerged fermentation of hericium erinaceus liquid, and hypha proliferation and polysaccharide synthesis conditions are optimized; and after fermentation is finished, extracting and purifying mycelium polysaccharide by adopting a choline chloride-lactic acid eutectic solvent system. According to the method, high-valued utilization of the edible fungus chaff and various agricultural byproducts is achieved, the content of beta-(1-> 3)-D-glucan in the prepared hericium erinaceus polysaccharide is larger than or equal to 55%, the immunocompetence (IC50) reaches 32 micrograms / mL, the hericium erinaceus polysaccharide is remarkably superior to commercially available products, and the hericium erinaceus polysaccharide can be directly used as functional food and medicine raw materials. The process is green and efficient, and has remarkable economic and environmental benefits.

The invention provides a recombinant cordyceps militaris strain with high yield of beta-glucan as well as a construction method and application of the recombinant cordyceps militaris strain, and belongs to the technical field of bioengineering and genetic breeding of edible fungi. According to the invention, a cordyceps militaris CmGls super-safe overexpression vector is constructed through a CRISPR-Cas9 technology, and then the cordyceps militaris CmGls super-safe overexpression vector is transferred into cordyceps militaris to obtain a recombinant cordyceps militaris strain with high yield of beta-glucan. According to the recombinant cordyceps militaris strain, membrane binding type beta-1, 3-glucan synthetase gene CmGls is accurately inserted into a safe port site CmSh1 of a cordyceps militaris genome, compared with a wild type cordyceps militaris strain, the growth rate of the recombinant cordyceps militaris strain is remarkably increased by 15% or above, and yellow phenotype and cell wall stress resistance are remarkably enhanced; after 6 days of submerged fermentation, the mycelium amount and the extracellular beta-glucan yield are respectively improved by more than 10%, and the strain has good application in improving beta-glucan synthesis.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement and a preparation method of the straw-based biochar composite slow-release fertilizer. The preparation method comprises the following steps: (1) uniformly mixing sheep manure and pigeon manure, mixing the mixed manure with crushed corn straw to obtain a total material, inoculating bacillus subtilis, and composting and fermenting to obtain a primary organic matter; (2) fermenting the primary organic matter by using aspergillus niger, trichoderma reesei and saccharomyces cerevisiae to obtain a submerged fermentation organic matter; (3) crushing the submerged fermentation organic matter to prepare particles; the composite coating powder is scattered into the submerged fermentation organic matter, coating is conducted, and the coated organic matter is obtained; and (4) uniformly mixing the coated fermented organic matter, biochar, desulfurized gypsum, humus soil, a water-retaining agent and ammonium dihydrogen phosphate to obtain the straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement. The organic fertilizer can improve the pH value of saline-alkali soil, reduce the salt content and increase the organic matter content.

The invention relates to novel high-oxidation-resistance fungus and grain ingredient powder produced through ganoderma lucidum fermented whole wheat and a preparation method and application of the novel high-oxidation-resistance fungus and grain ingredient powder, and belongs to the field of food processing.The method comprises the steps that ganoderma lucidum strains are subjected to liquid submerged fermentation and hypha crushing culture, and high-activity liquid strains are obtained; performing soaking and cooking pretreatment on the whole wheat; mixing the pretreated whole wheat with nutrient substances, sterilizing, inoculating the liquid strain, and fermenting in a special breathable bag; and drying and grinding the fermentation product to obtain the fungus grain ingredient powder. Lucid ganoderma is utilized to efficiently convert combined polyphenols with low bioavailability in whole wheat into free polyphenols, and active ingredients of the lucid ganoderma are cooperated, so that the total phenol content and the antioxidant activity of the product are remarkably improved. The mushroom grain ingredient powder can be applied to whole-grain foods such as steamed buns, noodles and bread, and has the characteristics of natural products, nutrient enrichment and the like.

The invention discloses an improved molasses culture medium and an application method of the improved molasses culture medium in increasing the yield of bird nest alkane diterpenoid high-activity compounds, and relates to the technical field of microbial fermentation. The invention aims to solve the problems of long production period, low product yield, large batch difference and difficulty in large-scale and process control when a solid culture medium is used for static fermentation in the existing production method. The method comprises the following steps: inoculating 0.1-0.5% of black egg nest fungus into a flat plate solid culture medium for culture, and then transferring into a liquid seed culture medium for shake culture to obtain a seed solution; inoculating the seed liquid into a fermentation tank filled with an improved molasses culture medium for deep fermentation to obtain fermentation liquid; mycelia are separated from the fermentation liquor, and a product rich in the bird nest alkane diterpenoids is obtained through washing, quick freezing, drying, smashing, extracting and concentrating. The invention can obtain the improved molasses culture medium and the application method of the molasses culture medium in increasing the yield of the bird's nest alkane diterpenoid high-activity compounds.

The invention relates to a preparation method of a biological agent synergistically produced by bacillus and lactobacillus plantarum, the biological agent and application, and relates to the technical field of bacteriocin preparation. The liquid culture medium contains yeast extract powder and glucose obtained by performing differential temperature asynchronous enzymolysis on starch slurry; s2, uniformly spraying the submerged fermentation liquid obtained in the step S1 on the surface layer of a solid fermentation culture medium within the thickness of 0.5-1 mm for primary fermentation, inoculating lactobacillus plantarum FJS003 into the surface layer of the solid fermentation culture medium within the thickness of 2-3 mm for secondary fermentation after the primary fermentation is finished, and obtaining the production biological agent after the secondary fermentation is finished. The prepared biological agent can effectively inhibit the growth speed of escherichia coli, salmonella and staphylococcus aureus, and meanwhile can effectively degrade aflatoxin B1 and the content of lead, arsenic, mercury and cadmium.

The application provides a Brevibacillus parabrevis GBW-M0 and application thereof in aquaculture. The Brevibacillus parabrevis GBW-M0 is classified as Brevibacillus parabrevis Brevibacillus laterosporus , and the preservation number is CGMCC No. 32335. The Brevibacillus parabrevis GBW-M0 is prepared through seed liquid preparation and submerged fermentation to obtain Brevibacillus parabrevis GBW-M0 fermentation liquor. The Brevibacillus parabrevis GBW-M0 fermentation liquor can reduce the concentration of hepatointestinal ciliates carried in the Penaeus vannamei, and is used for preventing and controlling microsporidian diseases of aquaculture animals. Meanwhile, the Brevibacillus parabrevis GBW-M0 fermentation liquor can inhibit the growth of Microcystis aeruginosa, and is used for treating blue-green algae blooms in aquaculture water bodies, thereby improving the success rate of aquaculture.

This invention discloses a method for increasing the yield of arachidonic acid (RAA) produced by *Morchella spp.* fermentation, relating to microbial fermentation technology. The method employs either solid-state fermentation or liquid-state shallow static fermentation. *Morchella spp.*, used for RAA synthesis, is inoculated onto the surface of a solid-state fermentation medium for solid-state fermentation, or inoculated into a liquid fermentation medium for liquid-state shallow static fermentation. Feeding is performed during fermentation until the RAA content in the *Morchella spp.* reaches its peak. This method can increase the RAA content in synthesized oils to 1.72 times that of deep fermentation, reaching a maximum of 80.93%. The yield of RAA synthesized by *Morchella spp.* per 1L of culture medium consumed is up to four times that of deep fermentation. This method requires less equipment investment, is simple to operate, has very low energy consumption, is pollution-free, and allows for large-scale production of RA-rich oils and microorganisms, showing broad application prospects in pharmaceutical intermediates, nutritional supplements, and animal feed.

The invention discloses a preparation method of a bacteriostatic and growth-promoting functional microbial fertilizer, and particularly relates to the field of agricultural biotechnology and microbial fertilizers, and the preparation method comprises the following specific preparation steps: functional strain screening, seed liquid preparation, liquid submerged fermentation, organic fertilizer matrix pretreatment, compound fermentation and post-treatment. According to the preparation method, the fermentation liquor with high activity and high concentration is obtained through an optimized liquid fermentation process, and then the fermentation liquor and the pretreated organic fertilizer matrix are subjected to compound fermentation, so that the multifunctional microbial fertilizer which is high in viable count, good in stability and capable of effectively preventing and treating soil-borne diseases and remarkably promoting crop growth at the same time is finally prepared. The use amount of a chemical fertilizer is greatly reduced; under the condition that the use amount of the chemical fertilizer is reduced by 30%, the crop yield can still be kept and even increased by 5%-10%, the investment of chemical pesticides and chemical fertilizers can be reduced by applying the fertilizer, non-point source pollution is reduced, and the soil micro-ecology is improved.

The application discloses a kind of degradable flexible conductive mycelium material method, belong to new material technical field.The application utilizes deep fermentation to prepare mycelium nanoparticle composite fermentation liquor, and is combined with solid fermentation, and the resistance value of the conductive mycelium material prepared is 30-40 Ω / cm, with excellent conductive performance, and after repeatedly stretching 100 times, resistance value fluctuation is about 10%, and the stability of guide point is excellent;In addition, degradation rate reaches about 70%, and can be recycled, compared with traditional conductive material, it will not produce a large number of electronic garbage, and reduce the dependence on earth resources, will not cause harm to the environment.Meanwhile, the breaking strength of the flexible conductive mycelium material prepared by the application reaches more than 25MPa, and the contact angle is >90°.Therefore, the conductive mycelium material prepared by the application is similar to leather in appearance, and the conductivity and flexibility are good, which can promote the development of intelligent textiles.

This invention discloses a *Tremella fuciformis* strain and its method and application for fermenting to produce *Tremella fuciformis* polysaccharide, belonging to the field of microbial fermentation technology. The *Tremella fuciformis* strain has the preservation number CGMCC No. 42524. This invention utilizes this strain for liquid submerged fermentation to produce *Tremella fuciformis* polysaccharide. Through synergistic optimization of the culture medium composition, the method of adding mycelial extract, and fermentation control parameters, a highly efficient fermentation system suitable for this strain was constructed. Results show that adding 10% mycelial extract to the basic fermentation medium can significantly increase the yield of *Tremella fuciformis* polysaccharide. This strain still exhibits good scale-up adaptability in a 200 L fermenter, with a mycelial dry weight of 65.03±0.25 g / L, a polysaccharide content of 50.88±0.11%, and a polysaccharide yield of 33.09±0.20 g / L. This invention provides a new strain resource and technical solution for the efficient, stable, and large-scale industrial production of *Tremella fuciformis* polysaccharide.

The invention relates to the field of food additive pigment extraction, in particular to a preparation method of monascus red pigment with low citrinin. According to the method for performing liquid submerged fermentation by adopting a monascus strain, vegetable oil is added into a culture medium, production of citrinin in the fermentation process is controlled in a mode of feeding hydrogen peroxide in the fermentation process, and monascus red pigment is extracted; the monascus purpureus is subjected to liquid culture, acid precipitation, filtration, washing and filtration, a filter cake is collected, and the filter cake is extracted and refined with food-grade ethanol. The content of citrinin in the monascus red pigment obtained by using the method is extremely low, the quality and safety of a monascus product are greatly improved, and the method has relatively high industrial application value and is beneficial to popularization and application of monascus red.

Fermentation processes are described herein. The processes include a) supplying a first fermentation tank with a fermentation broth; b) supplying gas from an external gas source to the first fermentation tank and to a second fermentation tank; c) during step b), venting gas from the second fermentation tank into the first fermentation tank via a fluid pathway, and venting gas from the first fermentation tank via a vent of the first fermentation tank; and d) during step b) and after step c), closing the vent of the first fermentation tank, and opening a vent of the second fermentation tank, to force the fermentation broth from the first fermentation tank into the second fermentation tank via the fluid pathway.

The present disclosure describes a method for obtaining a proprietary biocatalyst, called lyophilized enzyme preparation (PEL) via submerged fermentation (SmF) of Diutina rugosa yeast. The biocatalyst obtained by the process is also applied in synthesis reactions for the production of estolide-type biolubricants; biodiesel and polyol-esters-type biolubricants.

The invention discloses a process for fermenting threonine by using yeast protein cream prepared from corn steep liquor and fresh yeast milk, and belongs to the technical field of microbial submerged fermentation. The preparation process comprises the following steps: (1) fermenting and culturing saccharomycetes, centrifuging, and storing, so as to obtain fresh yeast milk; (2) sterilizing the corn steep liquor to obtain sterile corn steep liquor; (3) mixing and fermenting the sterile corn steep liquor and fresh yeast milk to obtain fermentation liquor; (4) evaporating and concentrating the fermentation liquor to obtain yeast protein paste; and (5) adding the yeast protein cream into a fermentation culture medium, and inoculating escherichia coli for fermentation to obtain fermentation liquor containing threonine. According to the invention, the corn steep liquor and the fresh yeast milk are mixed and fermented to prepare the yeast protein cream suitable for fermenting threonine, so that the growth of thalli can be obviously promoted, and the fermentation yield of threonine is increased.

The invention discloses a method for producing a high-activity iron stick yam flavored product based on a solid-liquid coupling fermentation technology, and belongs to the technical field of biological fermentation. The method comprises the following steps: firstly, pretreating iron stick yam to obtain yam slurry and yam residues; carrying out liquid submerged fermentation on the Chinese yam slurry by using lactobacillus bulgaricus and saccharomyces cerevisiae so as to enrich micromolecular nutrition and metabolites; meanwhile, hericium erinaceus is used for conducting solid-state fermentation on the Chinese yam residues so as to degrade fibers and generate mycelia and secondary metabolites; and finally, mixing the liquid-state fermentation product with the solid-state fermentation product, and performing after-ripening and drying to finally obtain the iron stick yam fermentation product which is rich in nutrition, high in active component, unique in flavor and easy to absorb. According to the invention, full-component and high-value utilization of the iron stick yam is realized, and the nutritional and health-care functions and economic benefits of the iron stick yam are remarkably improved.

The invention relates to a method for rapidly detecting adenosine in artificial ganoderma lucidum mycelium powder and application, and belongs to the technical field of medicine component extraction and detection. The invention discloses a method for rapidly detecting adenosine in artificial ganoderma lucidum mycelium powder, and the method is characterized in that the ganoderma lucidum mycelium powder is prepared by processing a dried mycelium product obtained by performing liquid submerged fermentation culture on porous fungus Ganoderma lucidum (Leyss.ex Fr.) Karst. The method for rapidly detecting adenosine in the artificial ganoderma lucidum mycelium powder comprises the following steps: (a) taking about 0.25 g of a sample, and adding 50 ml of 20% ethanol; (b) heating and refluxing at 100 DEG C for 1 hour; (c) performing microfiltration to obtain a test solution; and (d) injecting 5 [mu] l of the test solution into high performance liquid chromatography, separating by using an octadecylsilane chemically bonded silica chromatographic column and 7% acetonitrile as a mobile phase, and inspecting under the wavelength of a 260nm ultraviolet lamp. The method can be used for rapidly detecting the adenosine content in the artificial ganoderma lucidum mycelium powder.

The present application relates to a kind of Eupenicillium fusion strain and its fermentation culture and application, belong to strain fermentation technical field.The content of active ingredients such as cordycepin, polysaccharide in the secondary metabolites of Eupenicillium F2652 of the present application is significantly improved, realizes the conversion and enrichment of polysaccharide of Eupenicillium strain while improving the content of cordycepin, the marker component of Cordyceps genus fungi.The fermentation culture obtained by liquid submerged fermentation of Eupenicillium F2652, the advantages of both parents are fused, both retain excellent composite polysaccharide synthesis ability, and also fuse good cordycepin accumulation potential, possess the development high cordycepin content Eupenicillium culture, it is conducive to enrich its as functional feed additive in different animals Practical application.

The invention belongs to the technical field of biological fermentation, and particularly relates to a submerged fermentation extraction process method of ganoderma triterpenic acid. Preparing to obtain a seed solution; preparing a fermentation culture medium, and performing two-stage submerged fermentation on the seed solution to obtain a fermentation solution; filtering and separating the fermentation liquor to obtain mycelia containing intracellular ganoderma triterpenic acid and supernate containing extracellular ganoderma triterpenic acid, drying and crushing the mycelia, and carrying out ultrasonic and enzymolysis synergistic extraction to obtain an extracting solution; mixing the supernate and the extracting solution, filtering to obtain a clear solution, and purifying, concentrating and drying the clear solution to obtain a ganoderma triterpenic acid refined product. The method solves the problem of low yield of ganoderma triterpenic acid caused by uniform mycelium growth and ganoderma triterpenic acid synthesis environment, insufficient carbon source supply and limited induction effect in traditional submerged fermentation through innovative combination of staged fermentation, a fermentation composite carbon source, a composite inducer and beta-cyclodextrin.