791 results about "Submerged fermentation" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention relates to a method for preparing an edible fungus health-care product. The method comprises the following steps of: (A), inoculating edible fungus strains into a culture solution, and performing shake culture on a shaker to obtain strain liquid; (B), inoculating the strain liquid obtained in the step A into a liquid culture medium containing barley malt and soybeans, and performing aerobic culture or anaerobic culture to obtain fermented edible fungus mycelia and edible fungus fermentation liquor; (C), crushing cell tissues of the edible fungus mycelia obtained in the step (B), and extracting by using water to obtain a mycelium extracting solution; and (D), mixing the edible fungus fermentation liquor obtained in the step (B) and the edible fungus mycelium extracting solution obtained in the step (C), and diluting or concentrating a mixed solution to obtain the edible fungus health-care product. Due to the adoption of an industrial liquid submerged fermentation technology of edible fungi, a large number of edible fungus strains can be propagated in the liquid culture medium; and the preparation method is simple in process, stable in quality and low in cost.

The invention discloses a composite plant enzyme containing probiotics and an application of the composite plant enzyme. The composite plant enzyme comprises amylase, lipase, protease, cellulase, beta-dextranase, xylanase and lactase. The composite plant enzyme is obtained by discontinuously feeding materials and fermenting for a plurality of periods, selecting a plurality of types of fruits and vegetables, and edible and medical plants as fermentation substrates and taking a plurality of types of probiotics/yeasts as fermentation inoculation substances through a plurality of grades of deep fermentation. The composite plant enzyme contains seven enzymes needed by a human body at the same time so that the plant enzyme has the synergistic effects of promoting in-vivo metabolism and decomposing toxic substances; the plant enzyme contains a high-activity compound enzyme and has a wide acting condition, good adaptability, and good stability, acid-base resistance and high-temperature resistance; the plant enzyme can effectively and rapidly improve the biochemical reaction speed and can be used as a raw material to be applied to foods and chemicals for daily use.

The present invention relates to a method for preparing chitin and chitosan oligosac charide. The method comprises the steps that usual raw materials such as the crust of shrimp and crab, the insect crust or the fungal mycelia, etc. are micronized through the dry process or wet process; the carapace material of the obtained fine powder raw material is decalcified with the chemical process, and then is defatted and deproteinized with the method of micro-organism compound enzyme coarse enzyme liquid co-enzymolysis, and the insect and fungus fine powder thereof is directly defatted and deproteinized; a whole cell immobilizing bioreactor of a chitin deacetylase high-yield producing strain is prepared, to perform the circulatory deacetylation to the chitin and then obtain chitosan with corresponding degree of deacetylatoion; obligate anaerobic acid-producing bacterium and high-yield producing chitosan bacterium are utilized, the chitosan is submerged and fermented in the liquid, to obtain chitosan oligosaccharide with high water solubility. The present invention has the advantages that the method is helpful to fully utilize the resources, and makes the waste to the worth, at the same time, the default of the manufacturing process of the chemical process can be avoided, the production efficiency is improved, the energy is saved, the consumption is reduced, the byproduct with corresponding high value added can be produced, the comprehensive economic benefits of the relative secondary industry are obviously improved, the industrial development is promoted, and the multi-win effect is attained.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g/l.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.

The present invention discloses a method for producing chitin/chitosan from the shell of silkworm chrysalis, fly larvina, which comprises: to break into pieces to progress the shell of silkworm chrysalis, fly larvina by dry or wet method; React the fine grits obtained with the fat enzyme and protein enzyme from micro-organism by a enzymolysis reaction in order to to eliminate the fat and protein contained in the chrysalis / larvina raw material; bleach using two-step method; Collect the materials after bleach and to wash, dry materials to obtain chitin product; Obtain chitosan by to circulate to cast off Acetyl reaction in a Rhizopus oryzae full cell immobilization biology reaction vessel; Obtain height hydrosoluble shel oligosaccharide by liquid submerged fermentation chitosan using acid formers. The present invention improves the quality of the product and the efficiency of production; reduces greatly the energy cost, the usage of acid, alkali, oxidant and other harmful chemistry thing and the discharge of effluent, waste residue; produces byproducts of high value at the same time; utilizes the resources integratedly; and increases the benefits of production.

The invention discloses a culture medium and method for submerged fermentation of inonotus obliquus, relating to a culture medium and method for microbial submerged fermentation, and solving the problems of low yield of traditional inonotus obliquus submerged fermentation mycelia and low output of intracellular polysaccharide and extracellular polysaccharide of the inonotus obliquus as the activeingredient for the submerged fermentation. The culture medium for submerged fermentation is prepared from the following components: carboxymethyl cellulose, glucose, soybean meal, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and the balance of water. The method for the submerged fermentation comprises the following steps of: (1) preparing a culture medium for the submerged fermentation of inonotus obliquus; (2) inoculating the inonotus obliquus; and (3) carrying out submerged fermentation at two stages. By means of the culture medium and the method for the submerged fermentation of the inonotus obliquus in the invention, higher mycelium yield and intracellular and extracellular polysaccharides can be obtained. The mycelia polysaccharide obtained by the invention is 1.4 times higher than that of wild fruiting bodies, and the content of other active ingredients is higher than or equal to that of the wild fruiting bodies. The culture medium and method provided by the invention can be used for providing a rapid and abundant seed liquid for the large-scale artificial culture of the inonotus obliquus.

A method for increasing hydrogen peroxidase stability relates to a biological zyme used in clean production in textile industry. This invention takes the thermoascus aurantiacus IFO9862 as the initial strain to be prepared to get the hydrogen peroxidase after liquid deep fermentation to be ultrafitered, concentrated and added by certain stabilizers, NaCl, or glue or glycerol kept for 60days under 40deg.C its alive reservation is over 90%.

A (Rhizopus Oryzae)ME-F11, its mutation screening method and method for fermenting fumaric acid are disclosed. The preserving code is CCTCC NO: M 207066. The fumaric acid concentration reaches to 90-120g/L, total yield reaches to 90-95 wt% . It's simple and can be used for industrial production.

The present invention discloses a strain capable of producing poly-gamma-glutamic acid and a method for producing poly-gamma-glutamic acid by utilizing said strain. The described strain is X-003, a Bacillussubilis, CCTCC, NO:M202048. It adopts seed liquor preparation and liquid submerged fermentation process to produce and obtain the poly-gamma-glutamic acid product. The hereditary feature of said strain is stable, and its capacity for synthesizing poly-gamma-glutamic acid is strong, and the molecular weight of synthetic poly-gamma-glutamic acid can be up to above 10000 KD. The shake-flask fermentation level of poly-gamma-glutamic acid by utilizing said invented strain and its fermentation method can be up to 10 mg/ml, and the fermentation level of the fermentation tank with 80L is 30 mg/ml.

The invention discloses a compound enzyme preparation for ramie degumming, and a preparation method thereof. The method comprises the steps of taking bacillus cereus obtained through separation as an original strain, preparing high-enzyme-activity alkaline pectinase which contains glucanase and neutral protease through seed culture and liquid-submerged fermentation, and compounding an appropriate amount of xylanase and mannanase on the basis of the alkaline pectinase so as to prepare the compound enzyme preparation. The invention also discloses a ramie degumming process, and the compound enzyme preparation is applied to an enzyme refining step in the ramie degumming process. The process enables the alkaline pectinase and hemicellulase to play a role jointly by compounding a plurality of biological enzymes, so as to achieve the aim of degumming. The process can save acid-base raw materials, and has the advantages of mild treatment conditions, low cost, good fiber quality, little pollution on environment and the like.

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

The present invention relates to application of Bacillus subtilis ANSB060 (Preservation code number of CGMCC NO. 3440), especially to application of the bacterial strain in drinking water and/or feed of breeding animals. The Bacillus subtilis ANSB060 can be obtained by such techniques as repeated separation, purification and rejuvenation and the like; the Bacillus subtilis ANSB060 has the advantages of strong bioactivity, excellent health benefit property, and good stress resistance and the like. Fermentation liquor of the Bacillus subtilis ANSB060 obtained by the production technique like deep fermentation and the like is added to the drinking water or the feed for the animals; after entering into the animal intestinal canals, the bacterial strain can quickly activate, reproduce and form preponderant profitable strain such that the application of the Bacillus subtilis ANSB060 can reduce the harmful strain, adjust the intestinal microecological balance, substitute drugs like antibiotics, and improve the animal weight increment and the feed utilization ratio and the like.

A haw vinegar beverage for preventing diseases, building up body and beautifying face is prepared from haw through brewing, in which the deep liquid fermenting by microbes is used.

The invention relates to a process for producing 5'nucleotide. A penicillium citrinum strain M-71 is cultured by submerged fermentation to generate extracellular nuclease P1, 2.5 to 5 percent ribonucleic acid solution is degraded, of which, the degradation rate is more than 80 percent; foreign protein and residual ribonucleic acid are removed by using a high frequency vibrating screen, clean 5' nucleotide solution after impurity removal is subjected to ultrafiltration by a ceramic membrane to remove the macromolecular residual matters; and four columns filled with strong alkaline anion exchange resin are connected in series to one-time adsorb and elute and collect in multiple steps, four mononucleotide solutions are collected, and a nano filter membrane with the molecular weight of 300 daltons is selected for cutting, desalting, concentration, decoloration by active carbon, crystallization and drying to obtain the product. The process has the advantages of better separating four nucleotides, removing inorganic phosphorus by elution and ensuring the preparation of high-purity 5'nucleotide product, and according to HPLC tests, the purity of the product can reach between 98 and 102 percent.

The invention discloses an antrodia cinnamomea submerged fermentation compound product and a preparation method thereof. The preparation method comprises the following steps: preparing a submerged fermentation medium from poria cocos powder, Chinese yam powder, radix puerariae powder, bee pollen, soybean meal, yeast powder, glucose, dipotassium phosphate, magnesium sulfate and the like; carrying out submerged fermentation and cultivation on an antrodia cinnamomea mycelium fermentation liquid rich in antrodia cinnamomea polysaccharide; and carrying out supercritical CO2 technical extraction, enzymolysis, separation, filtration and concentration to prepare Chinese herbal medicine extraction liquids such as fingered citrons, sealwort, wolfberries, fructus amomi, emblic leafflower fruits, turnjujube and the like. According to the invention, the content of active components such as antrodia cinnamomea polysaccharide and the like is optimized and the preparation method is high in yield, short in time and low in cost, can be used for industrial production, is an efficient and environment-friendly bioengineering production technology and has remarkable development and application prospects.

The invention relates to a method for preparing a microbial feed additive. Feather meal, mannitol, glucose, sodium citrate, sodium chloride and the like are taken as raw materials, and inoculated with Bacullus subtilis YYW-1 with the number of CGMCC NO.2396 to prepare the microbial feed additive by submerged fermentation and spin flash drying technology. The active ingredients of the microbial feed additive are the Bacullus subtilis and cultures thereof, and the cultures contain metabolites such as keratinase capable of efficiently degrading feather. The product can be used in the breeding industry and the feed industry, and has the advantages of improving intestinal flora, improving digestion and absorption capacity of animals and the feed conversion and utilization rate, particularly promoting the digestion, absorption and utilization of protein materials such as the feather meal, and reducing cost.

The invention relates to a submerged fermentation culture method of straw mushroom liquid strains and culture mediums thereof. The invention solves the technical defects of the prior art, such as long mother species propagation time, poor hypha quality, long fungus ball production time, different fungus ball sizes, higher production cost and the like. The method is used for the submerged fermentation culture of the straw mushroom liquid strains. The method comprises the following steps: 1. carrying out mother species culture in a special culture medium of straw mushroom mother species; 2. carrying out transition culture in a special culture medium of straw mushroom stocks; 3. homogenizing the stocks to obtain homogeneous succulent strains; 4. carrying out primary shaking culture and amplification in a shaking liquid culture medium; 5. carrying out secondary shaking culture and amplification; and 6. carrying out fermentation treatment in a submerged fermentation liquid culture medium to obtain the finished products of the straw mushroom liquid strains. The invention relates to the special culture medium of straw mushroom mother species, the special culture medium of straw mushroom stocks, the shaking liquid culture medium and the submerged fermentation liquid culture medium.