63 results about "Submerged fermentation" patented technology

A vegetable anti-floating submerged fermentation tank comprises a tank body, and a sealing cover is arranged at the upper end of the tank body. The lower end of the tank body is communicated with a connecting pipe, the other end of the connecting pipe is connected with a lower three-way valve, one port of the lower three-way valve is connected with a drainage pipe, the other port of the lower three-way valve is connected with a circulating water inlet pipe, the other end of the circulating connecting pipe is connected with a circulating pump, and the circulating pump is connected with a circulating water outlet pipe; the other end of the circulating water outlet pipe is connected with an upper three-way valve, one port of the upper three-way valve is connected with a water inlet pipe, the other port of the upper three-way valve is connected with a water inlet main pipe, the other end of the water inlet main pipe is connected to the upper end of the tank body, and a plurality of floating plates are arranged in the tank body in the vertical direction; according to the invention, the fermentation environment of large-batch vegetable fermentation is improved, and during fermentation, adverse effects of external impurities, infectious microbes, oxygen and the like on fermentation can be avoided.

The invention relates to the technical field of microorganisms, and discloses a Streptomyces sp.HU154 and application thereof, the strain is named Streptomyces sp.HU154, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.28456, the strain comes from a farmland soil sample in the Hengqiao town in Changxian County, Huzhou City, Zhejiang Province, and the strain can be used for fermentation production of dequasol. After the strain is subjected to aerobic liquid submerged fermentation, the content of the diquathrin in fermentation liquor can reach 3850ug / ml, and the yield of the diquathrin produced by fermentation is high.

The present invention relates to a mycelium component derived from submerged fermentation of at least one fungal strain in three different media: a defined component medium producing component A, a synthetic medium producing component B, or a composite natural medium producing component C comprising a sidestream extract derived from an agricultural food sidestream. This unique mycelium composition is characterized by chemical, biological, physical, morphological, nutritional and organoleptic properties. The three different fibrous mycelia of the edible fungi obtained from at least one fungus strain are further used to produce characteristic food products, including meat analogs, fish analogs, dairy product analogs, beverages, or other food products. The three new mycelium components are useful in the manufacture of foods, foods, beverages, pharmaceuticals, cosmetics, nutraceuticals, biomaterials, and feed and industrial applications.

The invention discloses a method for efficiently preparing high-activity hericium erinaceus polysaccharide from waste in the edible mushroom industry, and belongs to the technical field of deep processing of edible mushrooms and recycling of waste. The method comprises the following steps: carrying out steam explosion pretreatment on hericium erinaceus waste mushroom residues, and carrying out synergistic compatibility on the hericium erinaceus waste mushroom residues, tea residues, bean residues and citrus residues which are subjected to specific enzymolysis to prepare a liquid fermentation culture medium; a two-stage control strategy is adopted for submerged fermentation of hericium erinaceus liquid, and hypha proliferation and polysaccharide synthesis conditions are optimized; and after fermentation is finished, extracting and purifying mycelium polysaccharide by adopting a choline chloride-lactic acid eutectic solvent system. According to the method, high-valued utilization of the edible fungus chaff and various agricultural byproducts is achieved, the content of beta-(1-> 3)-D-glucan in the prepared hericium erinaceus polysaccharide is larger than or equal to 55%, the immunocompetence (IC50) reaches 32 micrograms / mL, the hericium erinaceus polysaccharide is remarkably superior to commercially available products, and the hericium erinaceus polysaccharide can be directly used as functional food and medicine raw materials. The process is green and efficient, and has remarkable economic and environmental benefits.

The invention discloses a multi-level simulated meat fiber and a preparation method thereof, and belongs to the technical field of food. The preparation method of the multi-level simulated meat fiber comprises the following steps: preparing a potato glucose water culture medium; inoculating the liquid strain into a potato glucose water culture medium, and culturing for 7-8 days to obtain a liquid seed; inoculating the liquid seeds into a fermentation tank, and performing deep fermentation; filtering water and harvesting to obtain fermentation product mycelia; placing the mycelium in a water bath kettle for water bath; adding auxiliary materials, and uniformly mixing the auxiliary materials with the mycelia to obtain a mycelia mixture; performing freezing; unfreezing at room temperature, putting into an air fryer, and heating; and storing the heated hypha mixture under a refrigeration condition of 0-4 DEG C. The multi-level simulated meat fiber is finer and smoother, has real meat texture, and maintains the nutritional ingredients of the raw materials at the same time.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus buchneri strain LB-09 and application thereof. The preservation number of the lactobacillus buchneri strain LB-09 is CGMCC (China General Microbiological Culture Collection Center) No.31193, and the preservation date of the lactobacillus buchneri strain LB-09 is July 8, 2024. The lactobacillus buchneri strain LB-09 is subjected to submerged fermentation culture, fermentation liquor is applied to soil, leaf surfaces or seed surfaces, including soil application, leaf surface spraying, seed soaking or root dipping and the like, the disease resistance and the growth condition of crops are improved, and the lactobacillus buchneri strain LB-09 has the advantages of being good in biological safety, high in decomposition capacity, easy to apply and the like, and has a good application prospect. And green development and modernization of agriculture can be realized.

The invention relates to a method for producing a fungal fermentation medium from brewer's grains (BSG) and to a correspondingly obtainable fermentation medium, to a method for producing a fungal biomass by submerged fermentation of at least one fungal strain and to a correspondingly obtainable fungal biomass, and to food products obtainable by using the instant fungal biomass of the invention.

The utility model discloses natural plant effect micromolecule fermentation process display equipment for skin care products, which comprises a display cabinet, and a controller, a transparent material box and at least one process demonstration device are arranged in the display cabinet. The process demonstration device comprises a display screen, a first inductor, a first feeding pipe, a first material returning assembly, a plurality of first demonstration balls and a first driving mechanism, the two ends of the first feeding pipe are communicated with the material box and the first material returning assembly respectively, and the first inductor is arranged at the end, close to the display screen, of the first feeding pipe; when the first sensor senses the first demonstration ball, the controller controls the first driving mechanism to drive the first demonstration ball to return to the material box and controls the display screen to play a pre-stored video. Natural plant effect micromolecules are displayed to people through an electromechanical and multimedia combined closed system, and the process of enriching active substances and achieving the effect of enhancing the effect is achieved through submerged fermentation of microorganisms, so that people know and are familiar with the concept of plant skin care.

The invention belongs to the technical field of biology. The invention relates to the field of medicine, in particular to application of grifola frondosa fermentation liquor in preparation of medicine for treating cervicitis with high-risk human papilloma virus, gynecological gel for treating high-risk human papilloma virus infection and a preparation method and application of the gynecological gel. The grifola frondosa fermentation liquor is obtained by taking grifola frondosa as a fermentation strain and adding a phellinus igniarius extract into a fermentation substrate for liquid submerged fermentation. Tests prove that the grifola frondosa fermentation liquor can obviously inhibit the expression of E6mRNA in SiHa cells and HeLa cells; in a clinical test, the grifola frondosa fermentation liquor combined with the recombinant human interferon a2b has a remarkable curative effect on treating cervicitis with HPV infection, the HPV negative conversion rate can be effectively increased, the inflammatory factor secretion level can be reduced, the ecological restoration balance of the vagina microenvironment can be promoted, and yang recovery is not easy after treatment.

The invention relates to application of acremonium terricola F2652 in preparation of a preparation for improving the production performance, immunity or antioxidation of weaned piglets, and belongs to the technical field of strain fermentation. The contents of active components such as cordycepin, polysaccharide and the like in secondary metabolites of the acremonium terricola F2652 are obviously increased, so that the conversion and enrichment of the polysaccharide of the acremonium terricola strain can be realized while the content of a symbolic component cordycepin of cordyceps fungi is increased. The acremonium terricola F2652 is subjected to liquid submerged fermentation to obtain a fermentation culture, the advantages of amphiphilic parents are fused, the excellent compound polysaccharide synthesis capacity is reserved, the good cordycepin accumulation potential is also fused, and the acremonium terricola culture with the high cordycepin content can be developed; the practical application of the feed additive as a functional feed additive in different animals can be enriched.

The invention provides a recombinant cordyceps militaris strain with high yield of beta-glucan as well as a construction method and application of the recombinant cordyceps militaris strain, and belongs to the technical field of bioengineering and genetic breeding of edible fungi. According to the invention, a cordyceps militaris CmGls super-safe overexpression vector is constructed through a CRISPR-Cas9 technology, and then the cordyceps militaris CmGls super-safe overexpression vector is transferred into cordyceps militaris to obtain a recombinant cordyceps militaris strain with high yield of beta-glucan. According to the recombinant cordyceps militaris strain, membrane binding type beta-1, 3-glucan synthetase gene CmGls is accurately inserted into a safe port site CmSh1 of a cordyceps militaris genome, compared with a wild type cordyceps militaris strain, the growth rate of the recombinant cordyceps militaris strain is remarkably increased by 15% or above, and yellow phenotype and cell wall stress resistance are remarkably enhanced; after 6 days of submerged fermentation, the mycelium amount and the extracellular beta-glucan yield are respectively improved by more than 10%, and the strain has good application in improving beta-glucan synthesis.

The invention relates to an acremonium terricola fusion strain and a fermentation culture and application thereof, and belongs to the technical field of strain fermentation. The contents of active components such as cordycepin, polysaccharide and the like in secondary metabolites of the acremonium terricola F2652 are obviously increased, so that the conversion and enrichment of the polysaccharide of the acremonium terricola strain can be realized while the content of a symbolic component cordycepin of cordyceps fungi is increased. The acremonium terricola F2652 is subjected to liquid submerged fermentation to obtain a fermentation culture, the advantages of amphiphilic parents are fused, the excellent compound polysaccharide synthesis capacity is reserved, the good cordycepin accumulation potential is also fused, and the acremonium terricola culture with the high cordycepin content can be developed; the practical application of the feed additive as a functional feed additive in different animals can be enriched.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement and a preparation method of the straw-based biochar composite slow-release fertilizer. The preparation method comprises the following steps: (1) uniformly mixing sheep manure and pigeon manure, mixing the mixed manure with crushed corn straw to obtain a total material, inoculating bacillus subtilis, and composting and fermenting to obtain a primary organic matter; (2) fermenting the primary organic matter by using aspergillus niger, trichoderma reesei and saccharomyces cerevisiae to obtain a submerged fermentation organic matter; (3) crushing the submerged fermentation organic matter to prepare particles; the composite coating powder is scattered into the submerged fermentation organic matter, coating is conducted, and the coated organic matter is obtained; and (4) uniformly mixing the coated fermented organic matter, biochar, desulfurized gypsum, humus soil, a water-retaining agent and ammonium dihydrogen phosphate to obtain the straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement. The organic fertilizer can improve the pH value of saline-alkali soil, reduce the salt content and increase the organic matter content.

The invention provides a compound feed additive produced by liquid submerged fermentation and a preparation method thereof, the compound feed additive comprises xylanase and acid protease, and the compound feed additive is obtained by co-fermentation of aspergillus niger and kluyveromyces marxianus with soybean meal, defatted rice bran and inorganic salt as fermentation substrates. Kluyveromyces marxianus can eliminate xylose, amino acid and other feedback inhibition factors generated in the aspergillus niger fermentation process, aspergillus niger fermentation is promoted to generate xylanase and acid protease, and the fermentation enzyme activity is improved. After xylanase and acid protease generated in the growth and metabolism process of the aspergillus niger, bioactive substances such as alpha-galactosidase, pectinase and beta-glucanase which are generated along with the aspergillus niger and kluyveromyces marxianus are added into feed as additives at the same time, the digestion and absorption of livestock and poultry on nutrient substances and the health of animals can be promoted; and the application advantages of the product are improved.

The invention provides a preparation method of grape vinegar and the grape vinegar, and the preparation method of the grape vinegar comprises the following steps: carrying out liquefaction treatment and saccharification treatment on raisins to prepare grape juice sweet mash; carrying out enzymolysis treatment on the grape juice converted mash; carrying out alcoholic fermentation on the grape juice converted mash subjected to enzymolysis treatment by adopting a compound strain to prepare grape wine mash; wherein the composite strains comprise wine yeast and lactic acid bacteria, and the lactic acid bacteria comprise lactobacillus rhamnosus and / or lactobacillus plantarum; carrying out solid-liquid separation on the wine mash to prepare wine clear liquid; carrying out liquid submerged fermentation on the wine clear liquid by adopting acetic bacteria to prepare grape raw vinegar; wherein the acetic bacteria comprise acetobacter pasteurianus ZC501; and carrying out after-ripening treatment on the original grape vinegar by adopting an oak barrel to prepare the grape vinegar. The content of non-volatile acid and the content of total ester in the prepared grape vinegar are obviously increased, the flavor is obviously improved, the oak aroma is obvious, the whole aroma is rich, and the color is amber.

The invention relates to novel high-oxidation-resistance fungus and grain ingredient powder produced through ganoderma lucidum fermented whole wheat and a preparation method and application of the novel high-oxidation-resistance fungus and grain ingredient powder, and belongs to the field of food processing.The method comprises the steps that ganoderma lucidum strains are subjected to liquid submerged fermentation and hypha crushing culture, and high-activity liquid strains are obtained; performing soaking and cooking pretreatment on the whole wheat; mixing the pretreated whole wheat with nutrient substances, sterilizing, inoculating the liquid strain, and fermenting in a special breathable bag; and drying and grinding the fermentation product to obtain the fungus grain ingredient powder. Lucid ganoderma is utilized to efficiently convert combined polyphenols with low bioavailability in whole wheat into free polyphenols, and active ingredients of the lucid ganoderma are cooperated, so that the total phenol content and the antioxidant activity of the product are remarkably improved. The mushroom grain ingredient powder can be applied to whole-grain foods such as steamed buns, noodles and bread, and has the characteristics of natural products, nutrient enrichment and the like.

The present invention relates to a process for producing plant-based fermented food or feed containing plant protein with umami-rich sensory profile and improved nutritional profile, as well as products obtained therefrom and uses thereof. In particular the present invention relates to a process for producing fermented food or feed wherein the plant proteins are cross-fermented by the use of the combination of Propionibacterium spp., Lactobacillus spp., Aspergillus spp., and Saccharomyces spp. as well as products obtained therefrom and uses thereof. The process of the invention is based on the use of solid-state-fermentation (SSF) and submerged fermentation (SMF).

The present invention belongs to the technical field of enzyme engineering, and particularly relates to a lactase mutant and its application. Based on lactase molecules from two sources (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382), molecular evolution is carried out to obtain a new lactase enzyme molecule with high efficiency in synthesizing galactooligosaccharides and good expression performance; and a high-yield strain of lactase is constructed, which can efficiently synthesize lactase during deep fermentation and secrete the enzyme protein molecule into the culture medium, and directly prepare a high-activity enzyme preparation from the fermentation supernatant. The expression level of lactase can reach 2208 U / mL, which helps to reduce the fermentation manufacturing cost of lactase, simplify the fermentation manufacturing process and improve the quality of lactase enzyme preparation.

The invention discloses a method for preventing and treating rice blast by using compound microorganism fermentation liquor, and relates to the technical field of agricultural biologication.The method comprises the following steps that S1, bacillus strains with antagonistic activity on rice blast bacteria are selected for compound culture; the method comprises the following steps: respectively carrying out activation culture on selected bacillus of different species and strains to obtain respective seed solutions; and mixing the seed solutions according to a predetermined proportion, performing liquid submerged fermentation culture under controllable conditions, and controlling fermentation parameters to proliferate the compound microorganism thalli to a predetermined high concentration. According to the method for preventing and treating the rice blast through the compound microorganism fermentation liquor, the compound microorganism fermentation liquor preparation which can be directly used for spraying is prepared through compound culture of bacillus strains with antagonistic activity, so that the problems of drug resistance, pesticide residues and environmental pollution caused by chemical pesticides are effectively solved; the characteristics of greenness and environmental protection are shown, and the control effect on the rice blast is improved.

The invention relates to the technical field of manure fermentation, in particular to a heating and oxygenation device suitable for aerobic fermentation of solid manure, which comprises a fermentation frame, concave stacking frames are symmetrically hinged to two sides of the center in the fermentation frame, the bottoms of the stacking frames are of mesh structures, and guide frames are obliquely arranged on two sides of a front end frame body of the fermentation frame in a penetrating manner. Corrugated grooves are formed in the front inner wall and the rear inner wall of the fermentation frame, a double-tooth sliding plate with the side section of an inverted convex structure is arranged at the top end in the fermentation frame, and an auxiliary fermentation mechanism is arranged at the bottom end of the double-tooth sliding plate; the deep fermentation process of the feces is effectively accelerated through a cooperative treatment mode of dynamic dehydration, turning and crushing and intermittent oxygen supply, so that the problems of insufficient oxygen supply and insufficient fermentation in the traditional fermentation process are solved, and the aerobic fermentation efficiency of the feces is remarkably improved.

The invention discloses a clean vegetable tank-type submerged fermentation method. The method comprises the following steps: pre-treating vegetables, preparing yeast water, performing tank-type submerged fermentation, and taking out the vegetables subjected to tank-type submerged fermentation. The tank-type submerged fermentation comprises the following specific steps: adding yeast fermentation water into a fermentation tank; performing liquid circulation for the first time in the fermentation tank, wherein the liquid circulation is to continuously extract liquid from the bottom and pour the liquid into the tank from the upper part of the fermentation tank; when the first liquid circulation begins, the vegetables are added into the fermentation tank by several times, and saline water is added when the vegetables are added; after all the vegetables are placed, the fermentation tank is closed, and then first liquid circulation continues to be carried out. According to the invention, the consistency of vegetable submerged fermentation can be improved, and the product quality is improved.

The invention discloses an improved molasses culture medium and an application method of the improved molasses culture medium in increasing the yield of bird nest alkane diterpenoid high-activity compounds, and relates to the technical field of microbial fermentation. The invention aims to solve the problems of long production period, low product yield, large batch difference and difficulty in large-scale and process control when a solid culture medium is used for static fermentation in the existing production method. The method comprises the following steps: inoculating 0.1-0.5% of black egg nest fungus into a flat plate solid culture medium for culture, and then transferring into a liquid seed culture medium for shake culture to obtain a seed solution; inoculating the seed liquid into a fermentation tank filled with an improved molasses culture medium for deep fermentation to obtain fermentation liquid; mycelia are separated from the fermentation liquor, and a product rich in the bird nest alkane diterpenoids is obtained through washing, quick freezing, drying, smashing, extracting and concentrating. The invention can obtain the improved molasses culture medium and the application method of the molasses culture medium in increasing the yield of the bird's nest alkane diterpenoid high-activity compounds.

The present invention provides submerged fermentation processes of producing one or more polypeptide of interest, the process comprising: a) fermenting one or more microorganism that produces the one more polypeptide of interest in a fermentation broth, and b) adding at least one enzyme to the fermentation broth: OR co-expressing the at least one enzyme in the one or more microorganism and secreting the at least one enzyme into the fermentation broth in an amount sufficient to reduce the viscosity of the fermentation broth, compared to when the at least one enzyme is not added or co-expressed; and, optionally, c) recovering the one or more polypeptide of interest.

The invention relates to a preparation method of a biological agent synergistically produced by bacillus and lactobacillus plantarum, the biological agent and application, and relates to the technical field of bacteriocin preparation. The liquid culture medium contains yeast extract powder and glucose obtained by performing differential temperature asynchronous enzymolysis on starch slurry; s2, uniformly spraying the submerged fermentation liquid obtained in the step S1 on the surface layer of a solid fermentation culture medium within the thickness of 0.5-1 mm for primary fermentation, inoculating lactobacillus plantarum FJS003 into the surface layer of the solid fermentation culture medium within the thickness of 2-3 mm for secondary fermentation after the primary fermentation is finished, and obtaining the production biological agent after the secondary fermentation is finished. The prepared biological agent can effectively inhibit the growth speed of escherichia coli, salmonella and staphylococcus aureus, and meanwhile can effectively degrade aflatoxin B1 and the content of lead, arsenic, mercury and cadmium.

The invention relates to a microbial regulator, which is suitable for tobacco and rice crop rotation soil and comprises humic acid substances, natural diatomite, active lactic acid bacteria, phosphate dissolving bacteria, water and auxiliary materials. The humic acid substance is derived from cow dung compost and is naturally fermented; diatomite has high porosity and specific surface area. Microorganisms are cultured through liquid submerged fermentation, so that high viable count is ensured. The preparation method comprises the following steps: mixing, fermenting, adding water and auxiliary materials, and sterilizing. The auxiliary materials comprise trace element fertilizers and pH regulators, so that the soil environment is optimized, and the growth of crops is promoted. The conditioning agent can effectively improve the physicochemical properties of soil and enhance the fertility of the soil.

The invention discloses a special culture medium for culturing trichoderma reesei and producing cellulase for preparing hydrolysis sugar of lignocellulose biomass (represented by corncobs or straws and the like) and application of the special culture medium. According to the culture medium, a mixture of industrial glucose with low price and a cereal processing byproduct bran with lower price is used for replacing microcrystalline cellulose and lactose or whey powder which are commonly used at present and are high in price, so that the material consumption cost of cellulase liquid submerged fermentation production is remarkably reduced; the production cost of the cellulase is reduced to the technical and economic indexes required by hydrolysis of (semi) cellulose components in lignocellulose biomass represented by corncobs or straws and the like to prepare hydrolyzed sugar, and the special requirements of fermentation production of cellulosic ethanol, other bulk bio-based products and the like are met.

The application provides a Brevibacillus parabrevis GBW-M0 and application thereof in aquaculture. The Brevibacillus parabrevis GBW-M0 is classified as Brevibacillus parabrevis Brevibacillus laterosporus , and the preservation number is CGMCC No. 32335. The Brevibacillus parabrevis GBW-M0 is prepared through seed liquid preparation and submerged fermentation to obtain Brevibacillus parabrevis GBW-M0 fermentation liquor. The Brevibacillus parabrevis GBW-M0 fermentation liquor can reduce the concentration of hepatointestinal ciliates carried in the Penaeus vannamei, and is used for preventing and controlling microsporidian diseases of aquaculture animals. Meanwhile, the Brevibacillus parabrevis GBW-M0 fermentation liquor can inhibit the growth of Microcystis aeruginosa, and is used for treating blue-green algae blooms in aquaculture water bodies, thereby improving the success rate of aquaculture.

The invention belongs to the field of agricultural and forestry waste resourceful treatment, and discloses a camellia oleifera shell mycelium composite material and application thereof. The preparation method comprises the following steps: inoculating white rot fungus liquid seeds into a camellia oleifera shell solid-state fermentation substrate, uniformly mixing, filling into a mold, carrying out first-time solid-state fermentation, demolding, carrying out second-time solid-state fermentation until hyphae grow all over the solid-state fermentation substrate, and drying and shaping until a compact mycelium film is formed on the surface, so as to obtain the camellia oleifera shell mycelium composite material. According to the preparation method combining liquid submerged fermentation and solid state fermentation, the production efficiency of the mycelium composite material is remarkably improved, and the prepared composite material is hydrophobic in surface, good in compression resistance, rebound rate, heat insulation performance and flame retardant performance, wide in application range and capable of being used for preparing various heat preservation and insulation packaging and building materials.

The invention discloses an edible mushroom liquid submerged fermentation device, and relates to the field of edible mushroom fermentation devices.The edible mushroom liquid submerged fermentation device comprises a fermentation box body, a partition plate is fixedly installed on the inner wall of the fermentation box body, a supporting frame is fixedly installed on the upper surface of the partition plate, and a driving assembly is fixedly installed at the top of the partition plate; and one end of the driving assembly is fixedly connected with a stirring assembly. A first stirring rod and a second stirring rod are arranged in a staggered and inclined state, in the process that the stirring structure is separated from a culture medium, the stirring structure can mark an opening in the culture medium, air in the device can enter the culture medium from the opening, the effect of improving the oxygen solubility is achieved, and the oxygen solubility is improved. In this way, the problems that the oxygen diffusion speed is low and the oxygen diffusion range is small due to the fact that the viscosity of the culture medium is high are solved, the stirring structure in the culture medium can stir the culture medium at a low speed, and oxygen can be diffused to all positions of the culture medium.

The invention relates to an efficient liquid submerged fermentation culture medium of russula vinosa polysaccharide and a fermentation method, and belongs to the technical field of fermentation. The formula of the efficient liquid submerged fermentation culture medium for russula vinosa polysaccharide comprises the following components: glucose, a russula vinosa stem water extract, a schizochytrium limacinum degreased powder water extract, a nannochloropsis oculata degreased powder protein enzymatic hydrolysate, an anoectochilus formosanus leaf water extract, a soybean cake powder protein enzymatic hydrolysate, wort, proteoglycan, monopotassium phosphate and magnesium sulfate heptahydrate. On the basis of the fermentation culture medium, a gradient fermentation technology is matched, efficient fermentation production of russula vinosa polysaccharide is achieved by adjusting the components and nutrient concentration of the culture medium in stages, the bottleneck of current russula vinosa polysaccharide resource acquisition is broken through, and industrial application of russula vinosa polysaccharide is promoted.