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39 results about "Fungal biomass" patented technology

Fungal biomass provides a metal sink, either by metal biosorption to biomass (cell walls, pigments, and extracellular polysaccharides), intracellular accumulation, and sequestration, or by precipitation of metal compounds onto and/or around hyphae.

Fungi cultivation on alcohol fermentation stillage for useful products and energy savings

A method of processing stillage from fermentation derived alcohol is disclosed, including dry-grind ethanol production from corn, by fermentation with filamentous fungi. This produces high-value fungal biomass that can be recovered by screening, is easily dewatered and used as an animal feed, human food or as a source of nutraceuticals. The methodology uses an airlift reactor to enhance the morphology of the fungi for easy harvesting and separation of water for recycling and reuse and to recover added enzymes and mineral acid with the water. The process also separates oil from the stillage. The fungal processing removes organic substances from the water that are otherwise inhibitory to the reuse prospects for the water, i.e. suspended and dissolved organic matter, including glycerol, lactic and acetic acids. The process also separates oil from the stillage by enmeshing the oil in the fungal biomass and can produce more oil through cultivation of oleaginous fungi. This approach generates revenue from low value thin stillage, while substantially reducing stillage processing costs, mainly by averting the need for evaporation of the thin stillage.
Owner:VAN LEEUWEN JOHANNES

Fungi cultivation on alcohol fermentation stillage for useful products and energy savings

A method of processing stillage from fermentation derived alcohol is disclosed, including dry-grind ethanol production from corn, by fermentation with filamentous fungi. This produces high-value fungal biomass that can be recovered by screening, is easily dewatered and used as an animal feed, human food or as a source of nutraceuticals. The methodology uses an airlift reactor to enhance the morphology of the fungi for easy harvesting and separation of water for recycling and reuse and to recover added enzymes and mineral acid with the water. The process also separates oil from the stillage. The fungal processing removes organic substances from the water that are otherwise inhibitory to the reuse prospects for the water, i.e. suspended and dissolved organic matter, including glycerol, lactic and acetic acids. The process also separates oil from the stillage by enmeshing the oil in the fungal biomass and can produce more oil through cultivation of oleaginous fungi. This approach generates revenue from low value thin stillage, while substantially reducing stillage processing costs, mainly by averting the need for evaporation of the thin stillage.
Owner:VAN LEEUWEN JOHANNES

Integration of First and Second Generation Bioethanol Processes

The present invention presents an alternative to the direct implementation of an industrial scale second generation bioethanol process with the integration of the second generation into the existing first generation bioethanol processes, which aims to reduce the current barriers to process change / investments. In particular, the present invention relates to an integrated second generation process for producing bioethanol comprising at least one fungal cultivation stage for producing ethanol and fungal biomass. The present invention also relates to a novel fungal biomass, rich in protein and essential amino acids, which is produced with said integrated second generation process for producing bioethanol for use as a nutritious substitute for human and domestic animal use.
Owner:LANTMANNEN ENERGI

Processes for isolating chitin and chitosan from fungal biomass

Methods of extracting chitin and chitosan from fungal biomass using a solution of one or more ammonia compounds, amines, and / or alkaline silicate compounds. The solution dissolves and extracts amino acids, fatty acids and other carbohydrates from the fungal cells leaving chitin and / or chitosan, and the extractant may be recovered from the liquid by simple phase changes such as heating or cooling, dissociation into volatile components, distillation and / or solidification and separation of immiscible extractants. Further lipid removal may be achieved with one or more organic solvents, which may also be recovered by distillation.
Owner:VAN LEEUWEN JOHANNES

Purification of thin stillage from dry-grind corn milling with fungi

The present invention is directed to an improved method of processing thin stillage from ethanol production and / or other industrial processes to produce high value fungal biomass that can be recovered by simple means. The effluent is sanitized and recycled using a novel disinfection technique. This innovative approach generates revenue from low value thin stillage, while reducing wastewater purification costs.
Owner:VAN LEEUWEN JOHANNES PHD +1

Compositions and methods for mycotoxin decontamination of animal feed, food, soil and plants using biomass of filamentous fungi and its selected components

A method of rendering harmless mycotoxins contaminating food, animal feed and assisting infection of plant hosts by microbial parasites is proposed. The method comprises binding mycotoxins by a novel adsorbent, consisting partially or in full of the biomass of filamentous fungi or isolated fungal biomass components, such as chitin, chitozan and hyrdophobins, or fungal biomass enriched in its capacity to bind said mycotoxins using fungal species and strain selection, genetic engineering of fungi, modification of fungal fermentation conditions and downstream physical and chemical treatment of fungal biomass, such as milling or micronization in a dry state. The resulting adsorbent can bind a wide range of mycotoxins, including the ones difficult to bind (Ochratoxin, T-2, DON, NIV) using a current generation of mycotoxin adsorbents based on clay, resins, yeast and bacterial biomass. The adsorbent can be used as an animal feed additive, functional food additive and agricultural fungistatic / fungicide.
Owner:CUBENA

Glucosamine and method of making glucosamine from microbial biomass

Glucosamine suitable for human or animal consumption is disclosed. The glucosamine is derived from microbial biomass containing chitin. Suitable starting materials include substantially uniform microbial fungal sources, such as fungal sources derived from Aspergillus sp., Penicillium sp., Mucor sp. and combinations thereof. Methods of producing glucosamine by acid hydrolysis of fermented fungal biomass are also disclosed.
Owner:CARGILL INC

A preparation method of porous nitrogen-doped carbon material based on fungal biomass and application thereof

The invention belongs to the preparation field of biomass carbon material, in particular discloses a preparation method and application of porous nitrogen doped carbon material based on fungal biomass. The preparation method comprises the following steps of: adding urea into potassium hydroxide solution to obtain mixed solution, reacting, drying the mixed solution to obtain dried product, carbonizing the dried product in an inert atmosphere to obtain carbonized product, purifying, and obtaining porous nitrogen doped carbon material; In the mixed liquor, the mass ratio of the fungal cell accumulating oil to potassium hydroxide to urea is 1: 1; and the mass ratio of the fungal cell accumulating oil to urea is 1: 1. 2: 1- 2. As that method of the invention is simple in operation, low in costand easy to regulate the nitrogen element dope ratio, the prepared porous carbon material has good electrochemical properties such as high specific capacitance, stable rate performance and the like, and therefore has good application prospects in the field of supercapacitors and energy storage.
Owner:CENT SOUTH UNIV

Methods for producing glucosamine from microbial biomass

A more efficient method for producing glucosamine from microbial biomass such as, for example, fungal biomass is disclosed. In one embodiment, a method for obtaining glucosamine from fungal biomass includes providing fungal biomass containing chitin, such as Aspergillus, Penicillium, Mucor, or mushroom. The fungal biomass is then reacted in an acidic solution with an acid concentration of greater than about 20% by weight and at a reaction temperature greater than about 60 DEG C to convert the chitin to glucosamine. This reaction is performed for a period of less than 4 hours. The glucosamine is then separated from the acidic solution. In selected embodiments, dried fungal biomass is used to accelerate the reaction. This dried fungal biomass may, in some embodiments, have a moisture content of less than about 20% and in some cases less than about 13%.
Owner:技源集团有限公司

Glucosamine and method of making glucosamine from microbial biomass

Glucosamine suitable for human or animal consumption is disclosed. The glucosamine is derived from microbial biomass containing chitin. Suitable starting materials include substantially uniform microbial fungal sources, such as fungal sources derived from Aspergillus sp., Penicillium sp., Mucor sp. and combinations thereof. Methods of producing glucosamine by acid hydrolysis of fermented fungal biomass are also disclosed.
Owner:HWANG KI OH +5

Use of Rhizomucor pusillus (Lindt) schipper in methods for treating industrial wastewaters containing dyes

InactiveUS20080110826A1Solid waste disposalWater contaminantsRhizomucor pusillusWastewater
Use of a fungal biomass for treating industrial wastewaters containing at least one dye and related method, wherein:i. the fungal biomass contains at least the fungal species Rhizomucor pusillus (Lindt) Schipper;ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.
Owner:MARCOPOLO ENG +1

Method for producing sorbicillactone a

Methods for an improved culturing of the fungus Penicillium chrysogenum, in particular strain KIP 3201, for an optimised production of the anti-tumour natural compound sorbicillactone A (2), and derivatives thereof in large amounts as well as optimised methods for the recovery and purification thereof from the fungal biomass and from the culture medium are described. Furthermore, the biological activity of sorbicillactone A (2), and studies regarding the genotoxicity of the compound are described.
Owner:JULIUS MAXIMILIANS UNIV WURZBURG +1

Purification of thin stillage from dry-grind corn milling with fungi

The present invention is directed to an improved method of processing thin stillage from ethanol production and / or other industrial processes to produce high value fungal biomass that can be recovered by simple means. The effluent is sanitized and recycled using a novel disinfection technique. This innovative approach generates revenue from low value thin stillage, while reducing wastewater purification costs.
Owner:VAN LEEUWEN JOHANNES PHD +1

Method for producing glucosamine from microbial biomass

A more efficient method for producing glucosamine from microbial biomass such as, for example, fungal biomass is disclosed. In one embodiment, a method for obtaining glucosamine from fungal biomass includes providing fungal biomass containing chitin, such as Aspergillus, Penicillium, Mucor, or mushroom. The fungal biomass is then reacted in an acidic solution with an acid concentration of greater than about 20% by weight and at a reaction temperature greater than about 60° C. to convert the chitin to glucosamine. This reaction is performed for a period of less than 4 hours. The glucosamine is then separated from the acidic solution. In selected embodiments, dried fungal biomass is used to accelerate the reaction. This dried fungal biomass may, in some embodiments, have a moisture content of less than about 20% and in some cases less than about 13%.
Owner:TSI GROUP

Method for online in-situ monitoring of solid-state fermentation fungus biomass

The invention relates to a method for online in-situ monitoring of solid-state fermentation fungus biomass, which is characterized in that the method comprises the following steps of: carrying out fermentation test outside a reactor by using a production strain, shooting thalluses-matrix images at different fermentation stages, obtaining fractal dimension through digital image processing, simultaneously determining corresponding thallus biomass by using a chemical method and calculating a functional relation between the fractal dimension and the thallus biomass; shooting an thallus growth image in the solid-state fermentation process by using a built-in camera in the reactor, calculating the fractal dimension of the thallus growth image and obtaining corresponding biomass according to a functional relation between the fractal dimension and the biomass. Through the method, the online monitoring of the solid-state fermentation fungal biomass can be realized, and the automatic solid-state fermentation control can be promoted.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Method of selectively determining a fungal biomass

A method for selectively determining a fungal biomass by detection of a fungal enzymatic activity that is present in substantially all fungal species, such as enzymes involved in chitin metabolism (chitinase, chitin synthase, chitosanase, N-acetyl-glucosaminidase and beta-N-acetylhexosamidase). The invention can be used for detecting a fungal biomass in environmental samples, food products, plant material, building materials, industrial fungal cultures or sample from a human being or animal including blood samples. In particular, 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide is used as substrate for beta-N-acetylhexosamidase (EC 3.2.1.52). This enzyme activity correlates with the amount of fungal biomass present in a sample.
Owner:MYCOMETER

Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum

The invention discloses a preparation method of a plant essential oil emulsion for inhibiting growth of fusarium graminearum, which belongs to the technical field of food preservation. According to the method, plant essential oil is used as an oil phase, lecithin is used as an emulsifier, an acetate buffer solution is used as a water phase, and an emulsification method combining high-speed shearing and high-pressure homogenization is utilized to prepare the essential oil emulsion for inhibiting fusarium graminearum growth without adding an Ostwald ripening inhibitor. The plant essential oil emulsion prepared by the invention is uniform in dispersion and has relatively good physical stability. Meanwhile, other oil phases such as corn oil or MCT are not introduced into the plant essential oil emulsion disclosed by the invention; the plant essential oil disclosed by the invention can achieve a relatively good antibacterial effect at a relatively low concentration; when the emulsion concentration of the plant essential oil emulsion prepared from clove oil is 40 mL / L, the inhibition rate of fusarium graminearum is 75.7%, and a new method is provided for controlling fungal biomass and mycotoxin in the grain storage and processing process.
Owner:JIANGNAN UNIV

Verticillium dahliae oligosaccharyl transferase target gene fragment and interference vector and application thereof

The invention discloses a verticillium dahliae oligosaccharyl transferase complex STT3 subunit target gene fragment and an interference vector and application thereof, and belongs to the fields of clone and application of verticillium dahliae disease course key genes. A host-induced gene silencing technology is adopted to screen the target gene fragment which can obviously lower the disease index in oligosaccharyl transferase complex STT3 subunit genes, the Gateway interference vector is further established, and then stably inherited transgenic tobaccos of which the resistance to pathogenic bacteria is obviously improved are obtained; through the disease index, fungal biomass analysis and target gene transcriptional level detection, the oligosaccharyl transferase complex STT3 subunit target gene interference fragment with the optimal verticillium dahliae resisting effect is finally screened. The verticillium dahliae oligosaccharyl transferase complex STT3 subunit target gene fragment and the RNA interference vector can be applied to improving the disease resistance of a plant to verticillium dahliae and cultivating new transgenic plant species with the verticillium dahliae resistance.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Use of Rhizopus stolonifer (Ehrenberg) vuillemin in methods for treating industrial wastewaters containing dyes

Use of a fungal biomass for treating industrial wastewaters containing at least one dye, wherein:i. the fungal biomass contains at least the fungal species Rhizopus stolonifer (Ehrenberg) Vuillemin;ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.
Owner:MARCOPOLO ENG +1

Processes for isolating chitin and chitosan from fungal biomass

Methods of extracting chitin and chitosan from fungal biomass using a solution of one or more ammonia compounds, amines, and / or alkaline silicate compounds. The solution dissolves and extracts amino acids, fatty acids and other carbohydrates from the fungal cells leaving chitin and / or chitosan, and the extractant may be recovered from the liquid by simple phase changes such as heating or cooling, dissociation into volatile components, distillation and / or solidification and separation of immiscible extractants. Further lipid removal may be achieved with one or more organic solvents, which may also be recovered by distillation.
Owner:VAN LEEUWEN JOHANNES

Method for estimating fungal biomass during a traditional solid fermentation process by using biomarker

The invention discloses a culture-free biochemical method for estimating fungal biomass during a traditional solid fermentation process based on an ergosterol biomarker. The method comprises steps that: (1) a sample is pre-treated; (2) saponification is carried out; (3) a sample saponification liquid is extracted; (4) HPLC detection of ergosterol is carried out; and (5) the sample fungal biomass is analyzed and estimated. The method provided by the invention assists in providing fast and accurate analysis means for the researches of bacterial community structural compositions of traditional solid fermentation systems, and especially for the tracking of variation trends of the fungal biomass during the fermentation processes. The method provided by the invention assists in providing a theoretical basis for scientific regulation of traditional solid fermentation process. The method provides good social and economic benefits.
Owner:SICHUAN UNIV

Use of Rhizopus stolonifer (Ehrenberg) Vuillemin in methods for treating industrial wastewaters containing dyes

Use of a fungal biomass for treating industrial wastewaters containing at least one dye, wherein:i. the fungal biomass contains at least the fungal species Rhizopus stolonifer (Ehrenberg) Vuillemin;ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.
Owner:MARCOPOLO ENG +1

Use of cunninghamella elegans lendner in methods for treating industrial wastewaters containing dyes

Use of a fungal biomass for treating industrial wastewaters containing at least one dye, wherein: i. the fungal biomass contains at least the fungal species Cunninghamella elegans Lendner; ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.
Owner:MARCOPOLO ENG +1

A kind of cicada flower fungus organic fertilizer and preparation method thereof

The invention relates to a method for producing an organic fertilizer by using a by-product mycelium during isaria cicadae miquel production as a raw material. According to the present invention, theorganic fertilizer produced by the method has high nutrient content, and especially the organic matter content of more than 95%.
Owner:ZHEJIANG BIOASIA PHARMA CO LTD

Antipathogenic target gene fragment of Verticillium dahliae vdeg and its interference vector and application

The invention discloses an anti-pathogenic bacteria target gene fragment of verticillium dahlia VdEG and an interference vector and application thereof. A host induced gene silencing technology is adopted, a verticillium dahlia strain with high pathogenicity is used as a material to construct a plurality of interference plasmids of nicotiana fracture virus against exoglucanase of the target gene of verticillium dahlia, nicotiana benthamiana is transformed, and verticillium dahlia is inoculated by a method of agrobacterium injection, a Gateway interference vector is constructed in the target region which can obviously reduce the plant disease index to obtain stable genetic transgenic plants, and the fungal biomass and target gene transcription level are detected by the disease index and molecular biological methods to screen the best interference region. The screened VdEG target gene fragment and the Gateway interference vector constructed by adopting the target gene fragment can be applied to the improvement of the disease resistance of plants to verticillium dahlia and the cultivation of new varieties of anti-verticillium dahlia transgenic plants.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Verticillium dahliae adp-atp carrier protein disease course key target gene and its interference carrier and application

The invention discloses a verticillium dahliae ADP-ATP carrier protein (AACP) pathogenesis key target gene as well as an interference vector and application thereof, belonging to the fields of cloning and application of a verticillium dahliae pathogenesis key gene. According to the invention, 3 sections of pathogenesis key target genes which are capable of significantly reducing disease index in a plant are screened from an AACP gene by virtue of a host induced gene silencing technology, and the 3 sections of target segments are further used for constructing a Gateway interference vector, so as to obtain transgenic tobacco which is significantly high in pathogenic bacterium resistance and stable in genetic performance. Through disease index and fungal biomass analysis as well as transcriptional level detection of the target gene, an optimum interference section of the AACP gene is finally screened and obtained. The verticillium dahliae ADP-ATP carrier protein pathogenesis key target gene and the RNA interference vector disclosed by the invention are applicable to improving disease resistance of plants to verticillium dahliae and breeding new varieties of transgenic plants resisting verticillium dahliae.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Verticillium dahliae adenylate kinase target gene fragment and interference vector and application thereof

The invention discloses a verticillium dahliae adenylate kinase target gene fragment and an interference vector and application thereof, and belongs to the fields of clone and application of verticillium dahliae disease course key genes. A host-induced gene silencing technology is adopted to screen the target gene fragment which can obviously lower the disease index in adenylate kinase genes; the Gateway interference vector is further established, and then stably inherited transgenic tobaccos of which the resistance to pathogenic bacteria is obviously improved are obtained; through the disease index, fungal biomass analysis and target gene transcriptional level detection, the adenylate kinase target gene interference fragment with the optimal verticillium dahliae resisting effect is finally screened. The verticillium dahliae adenylate kinase target gene fragment and the RNA interference vector can be applied to improving the disease resistance of a plant to verticillium dahliae and cultivating new transgenic plant species with the verticillium dahliae resistance.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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