635 results about "Filamentous fungus" patented technology

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The present invention relates to a method for producing fermented edible plants or edible animal / plants, to fermented edible plants or edible animal / plants produced by same, and to foods containing same. The method for producing fermented edible plants or edible animal / plants includes the steps of: producing crushed edible plants or edible animal / plants; culturing a liquid mixture of grains, saccharides, filamentous fungi, and yeast for 24 to 36 hours to produce a mixed microbial broth; inoculating the edible plants or edible animal / plants with the mixed microbial broth, and firstly fermenting the edible plants or edible animal / plants for 3 to 8 days to produce first fermented edible plants or edible animal / plants; and inoculating the first fermented edible plants or edible animal / plants with bacteria, and secondly fermenting the first fermented edible plants or edible animal / plants for 6 to 12 days to produce second fermented edible plants or edible animal / plants. Whereby, a fermentation period can be shortened, and food deterioration and the growth of pathogenic microorganisms can be suppressed. Further, adding the fermented edible plants or edible animal / plants produced by the above-described method into foods can provide storage stability, increase bioavailability, and improve flavor.

Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.

A method of processing stillage from fermentation derived alcohol is disclosed, including dry-grind ethanol production from corn, by fermentation with filamentous fungi. This produces high-value fungal biomass that can be recovered by screening, is easily dewatered and used as an animal feed, human food or as a source of nutraceuticals. The methodology uses an airlift reactor to enhance the morphology of the fungi for easy harvesting and separation of water for recycling and reuse and to recover added enzymes and mineral acid with the water. The process also separates oil from the stillage. The fungal processing removes organic substances from the water that are otherwise inhibitory to the reuse prospects for the water, i.e. suspended and dissolved organic matter, including glycerol, lactic and acetic acids. The process also separates oil from the stillage by enmeshing the oil in the fungal biomass and can produce more oil through cultivation of oleaginous fungi. This approach generates revenue from low value thin stillage, while substantially reducing stillage processing costs, mainly by averting the need for evaporation of the thin stillage.

The subject invention pertains to polynucleotides encoding the enzyme oxalate decarboxylase from the filamentous fungus Aspergillus niger and methods of use. The subject invention also pertains to methods of using the enzyme oxalate decarboxylase from Bacillus subtilis.

Disclosed is a lighting device for use in the control of a plant disease, which has a light source capable of emitting light containing ultraviolet ray. The light source can emit both of UV-B having a wavelength component with a wavelength of about 280 to 340 um and UV-C in which a wavelength component with a wavelength of about 255 nm or less is cut off from wavelength components with wavelengths of about 100 to 280 nm to plant in a superimposed manner. The irradiation of a plant with the UV-C and the UV-B ensures to further inhibit the spore formation or the hypha growth of a filamentous fungus that causes a disease or the like, and to induce a resistance against a disease in the plant.

The invention discloses a complex probiotics for environmental remediation, a preparation method and application thereof. The complex probiotics is prepared from the following strains in weight proportion: 1 to 5 portions of photosynthetic bacteria, 1 to 3 portions of lactic acid bacteria, 0.5 to 1.5 portions of bacillus, 1.0 to 1.4 portions of actinomycete, 0.6 to 1.0 portion of microzyme, and 1 to 5 portions of filamentous fungus, wherein the contents of active bacteria in the strains are all between1,800 and 2,600 million per milliliter. The mechanism of action of the complex probiotics comprises that: the probiotics is used as a leading raw material and combined with other useful microbes to generate an oxidation resisting material, and the oxidation resisting material decomposes and oxidizes organic matters by oxidization, reduction, fermentation and the like, so that the harmful and poisonous materials are turned into unharmful and nontoxic materials, and the idea of changing wastes into valuables is realized. The complex probiotics is widely applied in environmental protection field of deodorizing in a washroom, reutilizing domestic garbage, treating odorous lakes and stagnant water, purifying tap water and a swimming pool and the like, and has low cost.

The present invention refers to a process to produce biodiesel and / or fuel oil from microbial oilseed and / or algal biomass and / or from sugar cane residues and derivatives. The products according to the present invention are appropriate for direct use in motors and to generate energy or steam. The integrated process of the present invention comprises the use, as raw materials, of microbial oil-producing biomass obtained from sugar cane residues and derivatives, which is integrated with algal biomass and / or glycerol and are processed by steps of production of oil-producing microbial biomass from filamentous fungi and / or yeasts, steps of simultaneous production of algal biomass by fully using residues, CO2 and residual broth of said production of microbial biomass, as well as steps of extraction and transesterification of lipids contained in the biomass, with reuse of the residual glycerol thus produced. The process as disclosed deals with innovative and ecologically sustainable technology, not generating any kind of residue, also providing for the advantage of releasing considerable volumes of oxygen into the atmosphere.

Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

The present invention relates to compositions comprising polypeptides and polysaccharides. The compositions are in general immune modulating. The invention also discloses methods of producing these compositions using filamentous fungi cultivated in liquid medium. The compositions are useful for example in the treatment of immune compromised conditions.

Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

A method is provided for improving the nutritional quality of a fibrous by-product or residue of a food manufacturing process, wherein the fibrous by-product or residue is inoculated with at least one filamentous fungus, and the fibrous by-product or residue is fermented thereby to decrease dry matter content, increase protein content, and decrease fat content of the by-product or residue. Suitable byproducts or residues include spent brewer's grains, dried distiller's grains, dried distiller's solubles, distiller's dried grains with solubles, residues of the cereal processing industry, wheat bran, soybean hulls, citrus pulp, beet pulp, rice husks or hulls, bagasse, apple pommace, and combinations thereof. Enzymes produced during the fermentation may also be used as valuable coproducts such as animal feed supplements, or may be used in primary fermentations for the brewing and distilling industry.

The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glyclosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.

The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

The invention supplies a method to produce organic fertilizer. The zymophyte agent is made up from brood cell bacilli, lactobacillus, photosynthetic bacteria, azotobacteria, yeast, filamentous fungus, and actinomycete. The expanding culture formula is 40% bean pulp, 30% wheat bran, 25% rice hull powder, 1.5% dextrose, 1% ZnSO4, 1% B, 1% MgSO4, and 0.5% peptone. The fermenting stockpile includes solid swine waste, 25% rice straw, 10% weathered coal, and 5% calcium superphosphate. The process steps includes: filtering swine waste, adding rice straw and weathered coal, adjusting carbon and nitrogen and water ratio, whisking to equal, inoculating microorganism fungus agent, high temperature fermenting, after being aged, drying, filtering, checking and packaging. The invention has the advantages of easy to operate, low cost, and less foul gas, etc.

The present invention relates to compositions comprising polypeptides and polysaccharides. The compositions are in general immune modulating. The invention also discloses methods of producing these compositions using filamentous fungi cultivated in liquid medium. The compositions are useful for example in the treatment of immune compromised conditions.

It is an object of the present invention to provide a method of adjusting productivity of enzymes, in particular, amylolytic enzymes, plant fiber degradation enzymes and proteolytic enzymes in a filamentous fungus culture product, by controlling releasing rate of nutrients from the culture raw material into the culture system when a filamentous fungus culture product is produced by culturing filamentous fungi in liquid medium containing as the culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa. The present invention provides a method of producing filamentous fungus culture product by using liquid medium containing as culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa comprising, culturing filamentous fungi while releasing rate of nutrients from the culture raw material into the culture system is controlled to adjust productivity of enzymes in the filamentous fungus culture product.

Described herein are protease inhibitors, variants thereof and methods for their production.

The invention discloses a method for making a fermenting bed for ecological pig breeding by using composite strain culture, in particular relates to a method for making the fermenting bed by using a plurality of microorganisms. The invention comprises the steps of preparing the culture and making the fermenting bed. In the invention, the culture is mainly prepared by microzyme, lactobacillus, actinomycetes, photosynthetic bacterium and mycelial fungus according to proportion. The culture after being activated is stirred uniformly with organic padding pro rata, piled, fermented, prepared into the fermenting bed, and then can be used for breeding live pigs. The method solves the problems of long fermenting starting time of the organic padding, unstable microbial inoculum, strict requirements on the fermenting conditions and materials as well as short service life in the existing ecological pig breeding method. The method uses the composite strain culture to build ecological colony house; the fermenting starting time of the organic padding is short, which can be 1 to 2 days in summer and 3 to 5 days in winter; the strain activity is strong; the decomposing process can be finished in 1 to 3 days; the effect lasts for long; and the fermenting bed can be continuously used for 5 to 8 years. Moreover, the method is green and environmental friendly.

The invention discloses a microbial deodorant as well as a preparation method and application thereof. The microbial deodorant comprises bacillus and filamentous fungus. The preparation method comprises the steps of: uniformly mixing bacillus fermentation liquor and filamentous fungus fermentation liquor, and preparing the mixed liquor into lyophilized powder according to the conventional method in the field; or only uniformly mixing microbial fermentation liquor and excipient liquor; or only uniformly mixing the bacillus fermentation liquor and the filamentous fungus fermentation liquor. The microbial deodorant is simple to prepare and can be used for quickly removing peculiar smells and simultaneously removing smelly-source substances completely.

An improved filamentous fungi is disclosed that has an enhanced nutritional profile by utilizing pulsed ultraviolet irradiation. According to the invention, the vitamin D component of mushrooms and other filamentous fungi may be drastically increased with no deleterious affects on appearance with the use of pulsed UV radiation. Mushrooms so treated had up to 1800% DV in one serving of fresh mushrooms.

The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.

The present invention relates to a method for producing citric acid from glycerol with a yield of more than 70%. The method comprises fermenting a filamentous fungus on a substrate which comprises glycerol. The advantage of the method according to the invention is that a widely available substrate, i.e. glycerol, can be used and that yields may be obtained which are much higher than the yields obtained using state of the art methods for producing citric acid from glycerol. The glycerol is used in combination with one or more other carbon sources.

The invention provides a method for synthesizing polyhydroxy alkanoates by excess sludge in water treatment, which takes into consideration of technological features of high-concentration organic acid waste water treatment, microorganism synthesis PHA treatment and sewage plant remaining sludge treatment, realizes high-concentration organic-acid-containing waste water treatment and reduces remaining sludge output. The method comprises the following steps: inoculating active sludge, restoring active sludge and biological selection phase; acclimatizing and accumulating PHA; discharging a batch of PHA-rich active sludge for extracting PHA. The leading characteristic of the method is simple and rapid, acclimatization process for each batch of inoculated sludge lasts no more than 15-30 days; the equipment is simple and the method can be realized by a single SBR reactor; in addition acclimatization process and accumulation process are synchronous; as for urban sewage plant secondary sludge, normal PHA content accounts for more than 30% of dry cell weight; furthermore, operation stability is good and an anaerobic selection process section is provided for ensuring stability of the reactor, so as to inhibit propagation of filamentous fungus to prevent sludge from bulking and collapsing.

The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

Disclosed is a lighting device for use in the control of a plant disease, which has a light source capable of emitting light containing ultraviolet ray. The light source can emit both of UV-B having a wavelength component with a wavelength of about 280 to 340 um and UV-C in which a wavelength component with a wavelength of about 255 nm or less is cut off from wavelength components with wavelengths of about 100 to 280 nm to plant in a superimposed manner. The irradiation of a plant with the UV-C and the UV-B ensures to further inhibit the spore formation or the hypha growth of a filamentous fungus that causes a disease or the like, and to induce a resistance against a disease in the plant.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.