624 results about "Filamentous fungus" patented technology

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The present invention relates to a method for producing fermented edible plants or edible animal/plants, to fermented edible plants or edible animal/plants produced by same, and to foods containing same. The method for producing fermented edible plants or edible animal/plants includes the steps of: producing crushed edible plants or edible animal/plants; culturing a liquid mixture of grains, saccharides, filamentous fungi, and yeast for 24 to 36 hours to produce a mixed microbial broth; inoculating the edible plants or edible animal/plants with the mixed microbial broth, and firstly fermenting the edible plants or edible animal/plants for 3 to 8 days to produce first fermented edible plants or edible animal/plants; and inoculating the first fermented edible plants or edible animal/plants with bacteria, and secondly fermenting the first fermented edible plants or edible animal/plants for 6 to 12 days to produce second fermented edible plants or edible animal/plants. Whereby, a fermentation period can be shortened, and food deterioration and the growth of pathogenic microorganisms can be suppressed. Further, adding the fermented edible plants or edible animal/plants produced by the above-described method into foods can provide storage stability, increase bioavailability, and improve flavor.

Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.

A method of processing stillage from fermentation derived alcohol is disclosed, including dry-grind ethanol production from corn, by fermentation with filamentous fungi. This produces high-value fungal biomass that can be recovered by screening, is easily dewatered and used as an animal feed, human food or as a source of nutraceuticals. The methodology uses an airlift reactor to enhance the morphology of the fungi for easy harvesting and separation of water for recycling and reuse and to recover added enzymes and mineral acid with the water. The process also separates oil from the stillage. The fungal processing removes organic substances from the water that are otherwise inhibitory to the reuse prospects for the water, i.e. suspended and dissolved organic matter, including glycerol, lactic and acetic acids. The process also separates oil from the stillage by enmeshing the oil in the fungal biomass and can produce more oil through cultivation of oleaginous fungi. This approach generates revenue from low value thin stillage, while substantially reducing stillage processing costs, mainly by averting the need for evaporation of the thin stillage.

The subject invention pertains to polynucleotides encoding the enzyme oxalate decarboxylase from the filamentous fungus Aspergillus niger and methods of use. The subject invention also pertains to methods of using the enzyme oxalate decarboxylase from Bacillus subtilis.

The invention discloses a complex probiotics for environmental remediation, a preparation method and application thereof. The complex probiotics is prepared from the following strains in weight proportion: 1 to 5 portions of photosynthetic bacteria, 1 to 3 portions of lactic acid bacteria, 0.5 to 1.5 portions of bacillus, 1.0 to 1.4 portions of actinomycete, 0.6 to 1.0 portion of microzyme, and 1 to 5 portions of filamentous fungus, wherein the contents of active bacteria in the strains are all between1,800 and 2,600 million per milliliter. The mechanism of action of the complex probiotics comprises that: the probiotics is used as a leading raw material and combined with other useful microbes to generate an oxidation resisting material, and the oxidation resisting material decomposes and oxidizes organic matters by oxidization, reduction, fermentation and the like, so that the harmful and poisonous materials are turned into unharmful and nontoxic materials, and the idea of changing wastes into valuables is realized. The complex probiotics is widely applied in environmental protection field of deodorizing in a washroom, reutilizing domestic garbage, treating odorous lakes and stagnant water, purifying tap water and a swimming pool and the like, and has low cost.

The present invention refers to a process to produce biodiesel and/or fuel oil from microbial oilseed and/or algal biomass and/or from sugar cane residues and derivatives. The products according to the present invention are appropriate for direct use in motors and to generate energy or steam. The integrated process of the present invention comprises the use, as raw materials, of microbial oil-producing biomass obtained from sugar cane residues and derivatives, which is integrated with algal biomass and/or glycerol and are processed by steps of production of oil-producing microbial biomass from filamentous fungi and/or yeasts, steps of simultaneous production of algal biomass by fully using residues, CO₂ and residual broth of said production of microbial biomass, as well as steps of extraction and transesterification of lipids contained in the biomass, with reuse of the residual glycerol thus produced. The process as disclosed deals with innovative and ecologically sustainable technology, not generating any kind of residue, also providing for the advantage of releasing considerable volumes of oxygen into the atmosphere.

Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.

Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

A method is provided for improving the nutritional quality of a fibrous by-product or residue of a food manufacturing process, wherein the fibrous by-product or residue is inoculated with at least one filamentous fungus, and the fibrous by-product or residue is fermented thereby to decrease dry matter content, increase protein content, and decrease fat content of the by-product or residue. Suitable byproducts or residues include spent brewer's grains, dried distiller's grains, dried distiller's solubles, distiller's dried grains with solubles, residues of the cereal processing industry, wheat bran, soybean hulls, citrus pulp, beet pulp, rice husks or hulls, bagasse, apple pommace, and combinations thereof. Enzymes produced during the fermentation may also be used as valuable coproducts such as animal feed supplements, or may be used in primary fermentations for the brewing and distilling industry.

The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glyclosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.

The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

It is an object of the present invention to provide a method of adjusting productivity of enzymes, in particular, amylolytic enzymes, plant fiber degradation enzymes and proteolytic enzymes in a filamentous fungus culture product, by controlling releasing rate of nutrients from the culture raw material into the culture system when a filamentous fungus culture product is produced by culturing filamentous fungi in liquid medium containing as the culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa. The present invention provides a method of producing filamentous fungus culture product by using liquid medium containing as culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa comprising, culturing filamentous fungi while releasing rate of nutrients from the culture raw material into the culture system is controlled to adjust productivity of enzymes in the filamentous fungus culture product.

Described herein are protease inhibitors, variants thereof and methods for their production.

An improved filamentous fungi is disclosed that has an enhanced nutritional profile by utilizing pulsed ultraviolet irradiation. According to the invention, the vitamin D component of mushrooms and other filamentous fungi may be drastically increased with no deleterious affects on appearance with the use of pulsed UV radiation. Mushrooms so treated had up to 1800% DV in one serving of fresh mushrooms.

The present invention relates to a method for producing citric acid from glycerol with a yield of more than 70%. The method comprises fermenting a filamentous fungus on a substrate which comprises glycerol. The advantage of the method according to the invention is that a widely available substrate, i.e. glycerol, can be used and that yields may be obtained which are much higher than the yields obtained using state of the art methods for producing citric acid from glycerol. The glycerol is used in combination with one or more other carbon sources.

The invention provides a method for synthesizing polyhydroxy alkanoates by excess sludge in water treatment, which takes into consideration of technological features of high-concentration organic acid waste water treatment, microorganism synthesis PHA treatment and sewage plant remaining sludge treatment, realizes high-concentration organic-acid-containing waste water treatment and reduces remaining sludge output. The method comprises the following steps: inoculating active sludge, restoring active sludge and biological selection phase; acclimatizing and accumulating PHA; discharging a batch of PHA-rich active sludge for extracting PHA. The leading characteristic of the method is simple and rapid, acclimatization process for each batch of inoculated sludge lasts no more than 15-30 days; the equipment is simple and the method can be realized by a single SBR reactor; in addition acclimatization process and accumulation process are synchronous; as for urban sewage plant secondary sludge, normal PHA content accounts for more than 30% of dry cell weight; furthermore, operation stability is good and an anaerobic selection process section is provided for ensuring stability of the reactor, so as to inhibit propagation of filamentous fungus to prevent sludge from bulking and collapsing.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.