45 results about "Gliocladium" patented technology

Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

The invention relates to the field of soil fertilizer and provides a biological organic repairing agent for soil pollution and its application and preparation method. The biological organic repairing agent of the invention is composed of, by weight, 1.5-3 parts of eymogenic bacterium, 1-1.5 parts of wheat bran, 1.5-2 parts of molasses meal, 45-50 parts of ammonium humate, 45-50 parts of hydrolase dregs, 150-200 parts of soybean meal, 100-150 parts of humic acid powder, 80-100 parts of zeolite powder, 25-40 parts of a calcium magnesium phosphate fertilizer, 150-200 parts of cattle and sheep manure, 150-204 parts of plant straw, 25-40 parts of potassium humate, 1.5-3 parts of bacillus mucilaginosus, 1-1.5 parts of Gliocladium roseum, 1.5-2 parts of Streptomycesvinaceus-drappus, 1-1.5 parts of trichoderma spp, 1-1.5 parts of Paecilomyces lilacinus, 1-1.5 parts of bacillus subtilis and 1-1.5 parts of micrococcus. According to the invention, soil pollution control, improvement and nutrition are integrated.

The invention discloses a gliocladium spp. strain for suppressing botrytis cinerea pers and relates to the gliocladium spp. strain. The invention provides a new strain of gliocladium spp., which can be used for suppressing the botrytis cinerea pers and have the effect of suppressing a plurality of other fungal diseases, thereby laying a material foundation for biological control of the botrytis cinerea pers and culture of new species for anti-botrytis cinerea pers by utilizing the biological technology. The gliocladium spp. strain for suppressing the botrytis cinerea pers is gliocladium roseumWY-1 which is collected in China General Microbiological Culture Collection Center with the collection No. of CGMCCNo. 1977. The gliocladium roseum WY-1 can significantly suppress pathogens of the botrytis cinerea pers, fulvia fulva, phytophthora parasitica, verticillium dahliae of tomato, fusarium wilt of tomato, valsa sordida nit, poplar fusarium wilt and blackcurrant leaf spot, and suppress the growth of mycelia of the pathogens.

The invention belongs to the technical field of microbes, and provides a pilot scale production method for gliocladium roseum chlamydospore by liquid fermentation, and the method comprises medium formula and fermentation parameter control. The liquid medium is composed of 40-50 g/L of cane sugar, 20-25 g/L of bean cake powder, 0.5-2 g/L of potassium dihydrogen phosphate, 0.2-1 g/L of magnesium sulfate and water, and the liquid medium is used for fermentation production of gliocladium roseum HLD-1 chlamydospore, wherein the inoculation amount is 0.2%-2% (volume ratio), the culture condition comprises that: pH is 4-6, the temperature is 26 DEG C-30 DEG C, the stirring speed is 180-250 r/min and the ventilatory capacity is 1:0.2-0.8. The culture time is calculated from inoculation, the spore is germinated when 8 hours pass by, and a large amount of chlamydospore is formed when the culture is 24-40 hours. When the time of liquid fermentation is 3-5 days, the concentration of chlamydospore is 1.5*108 per milliliter. The invention aims at providing a cheaper medium formula with abundant raw material source and an efficient easily-controllable liquid fermentation technology; and by using the method, the large-scale production of gliocladium roseum HLD-1 chlamydospore can be realized, and the method provides guarantee for large-area biocontrol application of gliocladium roseum HLD-1 chlamydospore.

The invention provides a fungus spore wettable powder which comprises diatomite 85-90%, lignin 1-30%, carboxymethyl cellulose 0.1-1%, sodium humate 0.01-0.2% and Gliocladium spp. spores powders having hyperparasitism actions against various plant pathogenic bacteria such as sclerotiorum, gray mold bacterium, sheath blight bacterium and root rot bacterium.

The invention provides a method for preparing a large quantity of chlamydospore of Gliocladium virens, which comprises a culture medium formula and culture technology. A liquid medium comprises the following compositions: 20 to 25 grams per liter of glucose, 1 to 3 grams per liter of bean cake powder, 1 to 3 grams per liter of urea, 0.005 to 0.05 gram per liter of ferrous sulfate, 0.75 to 1.5 grams per liter of dipotassium hydrogen phosphate and distilled water. The liquid medium is used for fermentation culture of Gliocladium virens which have superparasitic function on various plant pathogenic fungi (such as sclerotinia, gray mold, banded sclerotial blight and pine root fungi). The culture time is calculated beginning from inoculation, and the chlamydospore of the Gliocladium virens begins to be greatly generated after 36 to 48 hours and is respectively and independently separated. The invention aims to provide the culture medium formula with lower price and rich sources of raw materials, wherein a large quantity of the chlamydospore of the Gliocladium virens can be prepared by utilization of the culture medium formula; and the repeatability is good and the cost is greatly reduced under the condition of liquid fermentation culture, so as to guarantee the biological activity and the prevention and treatment effect of the spores in production and application.

The invention provides Gliocladium roseum chlamydospore and a method for producing wettable powder thereof, belonging to the technical field of biological pesticides. In the invention, the method of solid state fermentation is adopted, the fermentation temperature and oxygen supply are controlled to produce the Gliocladium roseum chlamydospore; and compost with the chlamydospore is dried and smashed to prepare the wettable power by adding a corresponding addition agent. The invention aims to provide a method for producing the Gliocladium roseum chlamydospore in a large scale at low cost to solve the problem that Conidium has short survival time and shelf life and ensures biological activity and preventive effects of the Gliocladium roseum chlamydospore in production and application. Meanwhile, the method solves the problem that spore recovery rate is low in the preparation process of the Gliocladium roseum chlamydospore and provides a method for preparing the wettable powder of the Gliocladium roseum chlamydospore, which features low cost and simpleness in operation. The Gliocladium roseum chlamydospore and the wettable powder thereof can be applied to treating multiple vegetable diseases, including gray mold, seed-borne diseases and soil-borne diseases.

The invention discloses a fungaltoxin ZEN degrading enzyme mutant and an application thereof, which belong to the technical field of bioengineering. The invention discloses a zearalenone degrading enzyme (ZENG enzyme for short) derived from Gliocladium roseum MA918, which is used as a parent, and histidine His at the 134 site and serine Ser at the 136 site are simultaneously replaced with phenylalanine Phe by using a gene mutation technology to obtain the double mutant H134F/S136F. Under the optimal catalytic condition, the residual enzyme activity of the enzyme is improved by 36% after the enzyme is subjected to heat preservation at 53 DEG C for 2 minutes; the residual enzyme activity after heat preservation for 5 min is improved by 33%; and the residual enzyme activity is improved by 12% after heat preservation for 7min, the residual enzyme activity is improved by 34% after the temperature of 58 DEG C is kept for 2 minutes, and after the temperature is kept for 5 minutes, the residual enzyme activity is improved by 13%.

The invention discloses a seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus. The seedling breeding and rapid propagation method includes collecting wild anoectochilus formosanus seedlings, cutting roots into small pieces prior to sterilizing and washing, cultivating the small pieces of the wild anoectochilus formosanus plants at the constant temperature of 25 DEG C to obtain purified mycorrhiza fungi gliocladium AR-11 or AR-13 mycena anoectochila to be served as symbiotic fungus of the anoectochilus formosanus, and finally realizing industrialization production of anoectochilus formosanus symbiotic seedlings. The MS culture medium is selected to be a seedling breeding medium, 6-BA cytokinin and NAA plant growth regulator are further added into the MS culture medium, a cultivation field to plant the anoectochilus formosanus is selected and managed scientifically; by the method, disease resistance and survival rate of tissue culture seeding of the anoectochilus formosanus can be improved, so that breeding speed is fast, and industrialized seedling production and mass production of the anoectochilus formosanus can be realized.

The invention relates to a method for control of tobacco nematode diseases with a composite nematicidal microorganism. The composite nematicidal microorganism is composed of the following four microorganisms: Gliocladium roseum, Paecilomyces lilacinus (Thom.) Samson, Burkholderia arboris and Sarratia entomophilus. Specifically, the Burkholderia arboris is Burkholderia arboris J211, and the preservation number is CCTCC NO:M 2019747. The Burkholderia arboris is a strain obtained by new research, and research finds that the strain has a high control effect on nematodes, and new functions are in continuous research; (2) on the basis of nematode control with the Burkholderia arboris, the other three strains are mixed, and the related volume ratio therein is controlled, so that the results of outstanding control effect and high control rate (shown as the embodiment) are realized; and 3) the composite nematicidal microorganism adopted by the method provided by the invention has obvious capability of improving the nematode disease resistance of field crops, and at the same time can improve commodity quality and yield of crops.

The invention provides glucamylase of which an amino acid sequence is SEQ ID NO:1. The invention discloses a glucamylase gene of gliocladium roseum for the first time. A shake flask fermentation experiment is carried out on recombinant bacteria, and the enzyme activity of a fermentation liquor can be up to 292U/ml. By adopting the glucamylase disclosed by the invention, an alpha-1,4-glucosidic bond can be efficiently decomposed from the non-reductive end of a carbohydrate chain, glucose can be hydrolyzed, and an alpha-1.6 glucoside bond also can be slowly hydrolyzed, and converted into the glucose, so as to be widely applied to the glucose industry.

The invention relates to a compound with poisoning pine wood nematode activity and its application, which belongs to the field of biological pesticide technology, using the method of normal solid fermentation and extraction separation by producting bacterial strains to get the said activity compound. The said producting bacterial strain is Gliocladium roseum (Gr87) and is preserved in CCCCM with the preservation number of CGMCCNo.0807. The said activity compound is made of six kinds of compound and poisons the pine wood nematode effectively, also prepares biotic pesticide for nematode single or combined.

The invention discloses an anti-disease microbial compound fertilizer and a preparation method thereof. The compound fertilizer is prepared by fermenting the following raw materials in parts by weight: 60-78 parts of amino acid liquid, 20-30 parts of nitrohumic acid, 1-10 parts of ammonium hydrogen carbonate and 1-3 parts of fermentation microbes, which are sufficiently mixed, stirred, fermented, charged and the like. The raw materials of the compound fertilizer are fermented by the fermentation microbes, so that a large amount of antibiotics such as Sankecuo, gliocladium roseum, coal slime toxin and embryonin can be produced to inhibit the growth of harmful bacteria; nutrients required for pathogenic bacteria are blocked by quick growth and propagation for competing for nutrients, occupying the space and consuming oxygen, and part of trichoderma strains are propagated in plant bodies to produce antibiotics, so that harmful bacteria are difficult to enter the plant bodies; and the raw materials are best resources for producing the anti-disease microbial compound fertilizer, and the yield increasing effect is significant.

The invention relates to a compound biocontrol microbial agent as well as a preparation method and an application thereof, which belong to the technical field of microorganisms. In order to solve theproblems of unstable control effect and narrow antibacterial spectrum range of a single strain, the invention provides a compound biocontrol microbial agent which comprises a trichoderma harzianum suspension and a gliocladium roseum suspension in a certain volume ratio, and the live bacteria number of the trichoderma harzianum suspension and the gliocladium roseum suspension is 106cfu/mL. The compound biocontrol microbial agent provided by the invention is wide in antibacterial spectrum, strong in antagonism, long in lasting period, low in production cost and simple in fermentation process, and can prevent and treat crop diseases caused by fungi such as botrytis cinerea, stem rot germs, rhizoctonia solani, anthracnose germs, fusarium and fusarium oxysporum when being applied to agricultural biological prevention and treatment; the microbial agent has a growth-promoting effect on crop seeds and seedlings, can promote seed germination, radicles and seedling growth, also has preservativeand fresh-keeping effects, and can reduce rotting rate and weight loss when applied to preservative and fresh-keeping of fruits and vegetables.

The microbial enzyme process of preparing D-pan-ethyl ether includes the following steps: splitting chemically synthesized DL- pantoic lactone to obtain D-pantoic lactone with filamentous fungi of Fusarium, Gibberella, Aspergillus, Penicillium, Rhizopus, Gliocladium, Aureobasidium, etc; and the subsequent reaction of D-pantoic lactone with 3-ethoxy propylamine to prepare D-pan-ethyl ether with high optical activity and high purity. The present invention has low preparation cost, simple technological process, high splitting efficiency and no environmental pollution.

Provided herein is Gliocladium isolate C-13 (NRRL 50072) which is an isolated strain of a Gliocladium spp. obtained from an endophyte of a Eucryphia cordifolia plant. Methods of culturing Gliocladium isolate C-13 are provided. The methods can include culturing the Gliocladium isolate C-13 under conditions sufficient to produce hydrocarbons. Also disclosed are methods for producing a hydrocarbon, a hydrocarbon fuel and a blend useful as a fuel. For example, the hydrocarbons produced from isolate C-13 can be used to generate biofuels, such as biofuels for vehicles and aircraft. Also provided are kits which comprise Gliocladium isolate C-13 and methods of cloning, isolating, and expressing Gliocladium isolate C-13 genes.

The invention relates to a multifunctional active organic fertilizer and a preparation method thereof, and relates to an organic fertilizer containing bacteria, fungi and spores, in particular to an active organic fertilizer with functions of soil restoration and pest and disease resistance. The aim is to provide a multifunctional active organic fertilizer for soil pollution restoration and product quality improvement and a preparation method thereof. The multifunctional active organic fertilizer comprises three layers from inside to outside, wherein the innermost layer is a component A whichcomprises bacillus subtilis and paecilomyces lilacinus as main active ingredients, the middle layer is a component B and a component C which are rich in various nutritive elements, and the outermost layer is a component D which comprises streptomyces vinaceus-drappus and gliocladium roseum as main active ingredients. The multifunctional active organic fertilizer can passivate heavy metals in the soil, activate the soil, improve the rhizosphere micro-ecological environment, degrade chemical pesticides and improve the crop quality. The organic fertilizer is used in the field of agricultural planting.

The invention provides a microorganism manure containing multi-strains, the manure is composed of organic manure and microorganism bacterium, the organic manure is prepared from the process of mixing the raw materials of livestock manure, crop stalk powder, rape seed cake and the like, then adding enzyme bacteria and then fermenting, drying and grinding, while controlling the water content between 10%-15%, the microorganism bacterium is a mixture prepared from bacillus subtilis, photosynthetic bacterium, nitrogen-fixing bacterium, streptomyces coelicolor, and pink gliocladium catenulatum. The manure adopts organic manure and microorganism bacterium as the constituents of the microorganism manure, improves greatly the living environment of the crop, not only provides nutrients for development requirements, but also increases the capability of adapting to environments.

The invention discloses a preparation method and application of a pleione bulbocodioides extract capable of efficiently inhibiting plant pathogenic fungi, and the method comprises the following steps:drying and pulverizing pleione bulbocodioides pseudobulbs, carrying out reflux extraction by using an aqueous ethanol solution, carrying out macroporous adsorption resin column chromatography purification, and performing concentrating under reduced pressure to dryness to obtain the pleione bulbocodioides extract. The preparation method is simple and easy to operate, the total yield is 10.5%-13.2%, the preparation method is suitable for large-scale production, and the obtained extract has remarkable inhibitory activity on plant pathogenic fungi and has good application prospects. The activityof the compound in inhibiting cabbage anthracnose pathogenic bacteria, guava anthracnose pathogenic bacteria and gliocladium tenuissimum is superior to that of an environment-friendly botanical pesticide ethylicin which is used in quantity at present.

A process for the preparation of a growth media for mass multiplication of bio-control fungi, comprising a chopped, shade-dried distillation waste in a plastic bag that is plugged with cotton followed by autoclaving and an inoculation of a strain of bio-control fungi such as Trichoderma harzianum or Gliocladium virens, and incubating the bags at room temperature for 14-30 days, and then shade drying and grinding the product to obtain a fine powder.

The invention discloses an organic fertilizer containing biological bacterial colonies and a preparation method of the organic fertilizer. The fertilizer is prepared from the following raw materials in parts by weight: 10-15 parts of organic matters, 10-15 parts of alkaline hydrolysis nitrogen, 8-13 parts of quick-acting potassium, 712 parts of quick-acting phosphorus, 5-10 parts of plant extract,10-15 parts of a fertilizer leavening agent, 10-15 parts of fermentation auxiliary materials, 5-10 parts of bone paste, 5-10 parts of biological bacteria, 5-10 parts of hide powder and 1-5 parts of bentonite. According to the invention, gliocladium virens, peniophora gigantea and trichoderma are added into biological bacteria to enhance the antifungal performance of the organic fertilizer produced by the invention, so that the fertilizer produced by the invention can better protect plants while having long fertilizer efficiency, and the survival rate of seedlings is improved; trace elements contained in the skin powder and the bentonite can improve the nutritional value of the fertilizer produced by the invention; and by adding the plant extract, the fertilizer can be more easily mixed with soil, so that plants can more easily absorb the fertilizer produced by the invention.

The present invention relates to a method for reducing wetwood content in green lumber by a treatment with a selected fungal species, such as Phlebiopsis gigantea or Gliocladium roseum, prior to kiln drying. In the laboratory conditions, the biological treated boards reduced wood moisture content (MC) by 22-37% more than untreated boards. The time required for drying biological treated boards was approximately reduced by 10% compared with untreated controls, and by 33% compared with freshly-saw lumber. After drying, the biological treated boards reduced the total deformation with stain, warping and checking by 14-25%.

The invention relates to the technical field of microorganisms, in particular to application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium. Under the large background that crop straw is fully returned to the field, the prepared gliocladium roseum NF-06 conidia are sprayed to inhibit generation of the fusarium graminearum perithecium, so that primary infection sources of wheat scab are reduced, and the green control purpose of forward control and comprehensive control of the wheat scab is achieved. The fusarium graminearum perithecium inhibition rate is high and is 80-90%, and the gliocladium roseum NF-06 and conidia are safe and free of pollution.

The present invention relates to gliocladin and its application, and belongs to the field of medical antibiotic technology. The gliocladin is obtained with the production strain and through conventional liquid or solid fermentation and extraction of the metabolite. The production strain is Gliocladium roseum Gr87 with preservation number of CGMCC No. 0807. The gliocladin has molecular expression of C21H16O2, and molecular weight 384. The gliocladin is used in preparing antibiotic for Shigella sonnei, tubercle mycobacillus, bacillus pyocyaneus, Shigella shigae, etc.