504 results about "Aqueous ethanol" patented technology

The invention discloses a preparation method of a V-shaped porous starch-grease inclusion compound. According to the method, V-type porous starch is used as a raw material and is blended with grease to prepare the starch-grease inclusion compound. The method specifically comprises the following steps: dispersing grease in a 60% ethanol aqueous solution, adding V-shaped porous starch, magnetically stirring for 2 hours at 25-30 DEG C, centrifuging the solution, washing the obtained precipitate twice by absolute ethyl alcohol, freeze-drying the washed precipitate, grinding, and sieving by a 100-mesh sieve, thereby obtaining the V-shaped starch-grease inclusion compound. According to the method, 60% ethanol is adopted as a solvent, efficient embedding of the grease is achieved under the normal temperature condition, and the adverse effect of a traditional heat treatment method on the oxidation stability of the grease is avoided. According to the method, on the basis of a spiral cavity structure and a honeycomb-shaped pore structure of V-shaped porous starch, grease is efficiently embedded in an inner cavity of the starch by utilizing a blending adsorption method, the content of free grease on the surface is reduced through the synergistic effect of physical adsorption and intermolecular acting force, and the stability of grease embedding is improved.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention discloses a wood-based composite phase-change material and a preparation method thereof, and belongs to the technical field of phase-change materials.The preparation method of the wood-based composite phase-change material comprises the following steps that wood is cut and then added into delignification liquid to be heated, the wood is taken out and washed with water, and delignified wood is obtained; the delignification wood is added into the modification liquid to be heated, and after being taken out, the delignification wood is washed and dried to obtain pretreated wood; mixing polyethylene glycol, citric acid and boric acid, heating and stirring to obtain a phase change material; dipping the pretreated wood into the phase change material for vacuum dipping, taking out and drying to obtain the phase change material. The modified liquid is prepared from an ethanol water solution, a modified filler, 2-(3, 4-cyclohexene oxide) ethyltrimethoxysilane and triethylamine. The delignification liquid and the modification liquid are adopted for pretreating the wood, so that the wood can be better firmly combined with the phase change material, leakage is prevented, and the finally prepared wood-based composite phase change material has excellent heat management capacity and stability.

The invention provides a preparation method and application of low-bath-ratio and high-viscosity hydroxypropyl carboxymethyl composite starch ether, and relates to the technical field of starch deep processing. The method comprises the following steps: S1, premixing corn starch with a compound base catalyst and magnesium stearate, and spraying an ethanol water solution for alkalization; s2, adding epoxypropane to carry out first-stage etherification, and then adding sodium chloroacetate to carry out second-stage etherification; and S3, after the reaction is finished, performing vacuum drying, crushing and sieving to obtain the high-viscosity hydroxypropyl carboxymethyl composite starch ether. Wherein the composite alkali catalyst comprises sodium hydroxide, sodium carbonate and calcium oxide. The starch ether obtained by the method has the advantages of high substitution degree, high viscosity, low solvent consumption and low energy consumption, and overcomes the defects of the traditional semi-dry method.

The invention discloses a preparation method and application of a bimetallic sulfide / three-dimensional porous carbon catalyst, and relates to the technical field of electrolyzed water catalysts.The preparation method comprises the following steps that melamine sponge is cleaned, and the clean melamine sponge is obtained; the preparation method comprises the following steps: dissolving metal salt 1 and metal salt 2 in an ethanol aqueous solution, and adding alkali and an aniline monomer to obtain a precursor solution; completely soaking the clean melamine sponge in a precursor solution, carrying out constant-temperature hydrothermal reaction, and sequentially washing and drying to obtain the melamine sponge anchored with bimetal ions; introducing inert gas for protection, and performing high-temperature vulcanization treatment to obtain the three-dimensional porous carbon catalyst embedded with the bimetallic sulfide heterostructure. The catalyst comprises uniformly dispersed bimetallic sulfide nanoparticles and a spongy three-dimensional porous carbon carrier, shows excellent bifunctional catalytic activity and stability when being applied to an electro-catalytic water decomposition reaction, and has a wide application prospect.

This invention belongs to the field of membrane material preparation and modification technology, and relates to a method for pre-wetting the surface of a hydrophobic ceramic membrane. The ceramic membrane is immersed in a cyclohexane solution of perfluorooctyltrimethoxysilane for 3-4 hours. After immersion, the ceramic membrane is dried in an oven. An ethanol solution is forced to circulate through the ceramic membrane to pre-wet the separation layer. The pre-wetting process proposed in this invention is simple, highly controllable, and easily scaled up to pre-wet multi-channel hydrophobic ceramic membranes. This invention achieves coarse adjustment of the wetting degree by controlling the concentration of the ethanol-water solution, and further fine adjustment by combining the circulation time of the ethanol-water solution, thus achieving more precise control of the wetting degree and optimal improvement of the uniformity of the hydrophilic coating.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses a gradient alcohol extraction preparation method of compound honeysuckle flower drops for improving T cell activity, and relates to the technical field of traditional Chinese medicine compound preparations, honeysuckle flower and radix astragali are selected for pretreatment, an ethanol aqueous solution and an interaction regulating agent are added for infiltration, an online traditional Chinese medicine compound preparation HPLC is adopted for dynamic alcohol concentration adjustment and temperature extraction under monitoring of the traditional Chinese medicine compound preparation, the method disclosed by the invention has the advantages that the interaction regulating agent is added in the raw material infiltration stage to block bad interaction among active components, and the online monitoring system is utilized to dynamically adapt the ethanol concentration and the extraction temperature to realize step-by-step efficient dissolution of the multi-polar components; the antioxidant is matched to inhibit oxidation of thermosensitive components, and the solubilizer assists dissolution of insoluble components, so that the core defect that synergistic extraction of multi-polar active components, degradation of the thermosensitive components and interaction inhibition of the components in the compound honeysuckle flowers cannot be considered in the prior art is overcome, and loss or antagonism of the active components is effectively avoided.

The invention belongs to the technical field of colored glazed glass, and particularly relates to colored glazed glass and a preparation method thereof. The preparation method of the colored glazed glass comprises the following steps: (1) selecting a glass sheet, cleaning, cutting and edging for later use; (2) printing glaze on the outer surface of the glass treated in the step (1) by adopting a silk-screen printing process, and drying; (3) carrying out toughening treatment on the glass treated in the step (2); (4) soaking the glass treated in the step (3) in an ethanol water solution containing a silane coupling agent, washing and drying for later use; and (5) taking two pieces of glass treated in the step (4), coating the inner surfaces of the two pieces of glass with a sizing material, and curing to obtain the colored glaze glass. The adhesive film of the colored glazed glass has good adhesive property and mechanical property, prevents the glass from being broken and splashed, and can resist the erosion of the external environment, maintain the adhesive property and mechanical strength of the adhesive film and prolong the service life of the colored glazed glass.

The invention relates to the technical field of skin care product raw materials, and particularly discloses a soothing anti-inflammatory composition as well as a preparation method and application thereof. The preparation method of the soothing anti-inflammatory composition comprises the following steps: step 1) mixing saffron crocus, radix sophorae flavescentis, houttuynia cordata, moringa seeds and astragalus membranaceus to obtain a composite raw material; the invention discloses a soothing and anti-inflammatory composition and a preparation method thereof.The soothing and anti-inflammatory composition and the preparation method thereof.The soothing and anti-inflammatory composition has the advantages that soothing, anti-inflammatory and skin barrier repairing effects are further improved.

The invention belongs to the technical field of agricultural and sideline food processing, and discloses sweet potato pectin with high intestinal fermentation characteristic based on a magnetic induction electric field, and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing starch-removed sweet potato residues with an acid solution, performing magnetic induction electric field treatment, and centrifugally collecting supernate; s2, mixing and stirring the supernate obtained in the step S1 and an ethanol water solution, then carrying out alcohol precipitation standing treatment, and centrifugally collecting a precipitate; mixing the precipitate with water, and performing dialysis and concentration treatment to obtain a concentrate; and carrying out freeze drying treatment on the concentrate to obtain the sweet potato pectin. According to the method, a magnetic induction electric field technology is combined with traditional acid solution extraction, so that the intestinal fermentation characteristic of the sweet potato pectin is effectively enhanced.

The invention belongs to the technical field of catalyst preparation, and particularly relates to a method for synthesizing a fly ash-based hierarchical porous ZnY / Si-Beta catalyst by adopting a solvent-free method and application of the fly ash-based hierarchical porous ZnY / Si-Beta catalyst. According to the invention, fly ash is taken as a raw material, the ZnY / Si-Beta catalyst is synthesized by adopting a solvent-free method to catalyze direct conversion of an ethanol aqueous solution to prepare butadiene, so that resource utilization of fly ash can be realized, the influence of water on the active center of the catalyst can be inhibited, and efficient conversion of the ethanol aqueous solution to prepare butadiene is realized.

This invention discloses a flavoring microcapsule for heated tobacco products, its preparation method, and its application, belonging to the field of heated tobacco technology. The preparation method includes the following steps: dissolving zein in an aqueous ethanol solution to obtain a zein solution; dissolving chitosan in an aqueous acetic acid solution to obtain a chitosan solution; then adding heterocyclic flavorings, polyvinyl alcohol, and antioxidants to the chitosan solution and mixing to obtain a flavoring solution; mixing and stirring the zein solution and flavoring solution evenly; then emulsifying under cold conditions and freeze-drying to obtain the flavoring microcapsule for heated tobacco products. The preparation method of this invention can obtain heterocyclic flavoring microcapsules with good morphology, high heterocyclic flavoring encapsulation rate, and high flavor loading capacity. When used in heated tobacco products, these microcapsules exhibit a suitable sustained-release rate, long-lasting aroma, and good consistency in each puff; moreover, the preparation method is simple, safe, and environmentally friendly.

The invention relates to the technical field of asphalt mixture processing, in particular to a high-asphalt-aggregate-ratio durable basalt fiber asphalt mixture with low-temperature crack resistance and a preparation method of the high-asphalt-aggregate-ratio durable basalt fiber asphalt mixture. The preparation method comprises the following steps: (1) soaking cleaned basalt fibers in a hydrochloric acid solution for pretreatment to obtain pretreated basalt fibers; (2) adding nano-hydroxyapatite, carboxymethyl cellulose and dodecanoic acid into an ethanol water solution for heating reaction, and collecting a product after purification; (3) adding the product into acetone, then adding the pretreated basalt fibers, and stirring to obtain modified basalt fibers; and (4) uniformly mixing the modified basalt fiber, the asphalt, the mineral powder and the aggregate in parts by weight, thereby obtaining the composite material. The preparation method is simple and easy for large-scale production, and the prepared mixture has excellent low-temperature crack resistance, water stability and high-temperature stability, which is of great significance for improving the quality of road engineering and prolonging the service life.

The invention provides tamarind polysaccharide gel and a preparation method and application thereof.The preparation method of the tamarind polysaccharide gel comprises the following steps that S1, beta-galactosidase is added into a tamarind polysaccharide aqueous solution, alcohol precipitation and drying are performed after enzymolysis and enzyme deactivation, and tamarind polysaccharide subjected to enzyme treatment is obtained; wherein the enzymolysis time is 1 to 2 hours; s2, adding the tamarind polysaccharide subjected to enzyme treatment into an ethanol water solution, and stirring to obtain a mixed solution; wherein the volume ratio of the ethanol in the mixed solution is less than or equal to 15%; s3, refrigerating the mixed solution to obtain the tamarind polysaccharide gel. According to the method, stable gel can be formed under the condition of low ethanol content, the irritation of the product is reduced, the strength of the gel can be considered, and the application range of the tamarind polysaccharide can be expanded.

The invention provides a drilling fluid and a preparation method thereof. The drilling fluid comprises the following raw materials: water, bentonite, sodium hydroxide, a filtrate reducer, an inhibitor, potassium chloride, a tackifier, a lubricant, a plugging agent, a film-forming agent and barite. Wherein the film-forming agent is prepared from the following raw materials in parts by weight: 50 to 60 parts of organic silicon solvent, 80 to 100 parts of ethanol water solution, 10 to 15 parts of polyurethane particles and 10 to 15 parts of nano silicon dioxide. The drilling fluid provided by the invention can prevent the drilling fluid or filtrate thereof from entering fine and micro-nano cracks of a shale well section, so that the influence of hydration or capillary force on a shale stratum is reduced, and the well wall stability problem in shale drilling can be fundamentally solved.

This invention discloses a method for preparing sodium houttuynia cordata, comprising the following steps: adding sodium methoxide and toluene to a reaction vessel, heating, then slowly adding nonanone and ethyl formate, and after the addition is complete, reacting with a gradient cooling process, and post-processing to obtain an aqueous phase containing intermediate I; adding the aqueous phase containing intermediate I and toluene to the reaction vessel, stirring, cooling, and rapidly introducing liquid sulfur dioxide, continuously stirring and reacting at 0-10°C for 5-15 minutes, cooling, and filtering to obtain crude sodium houttuynia cordata. The preparation method of this invention directly introduces liquid sulfur dioxide during the sulfonation reaction, greatly shortening the process time, while achieving high product yield and purity, and requiring less sulfur dioxide. Furthermore, in the post-processing of sodium houttuynia cordata, adding toluene to the reaction solution beforehand can effectively transfer the generated impurities to the toluene; recrystallization using a 50 v / v% ethanol aqueous solution removes water-soluble and oil-soluble impurities while further reducing the risk of fire and explosion during centrifuge operation.

The application discloses a method for extracting ketone ester from ginkgo leaves, products and applications thereof, and relates to the technical field of plant extraction. The method comprises the following steps: uniformly mixing ginkgo leaf dry powder with lime water suspension, then performing hydrothermal extraction, then separating to obtain filtrate, and adjusting the pH value of the filtrate to neutral to obtain pH-neutral filtrate; using a macroporous resin to perform adsorption and elution treatment on the pH-neutral filtrate to obtain eluate, and performing drying treatment on the eluate to obtain ginkgo leaf ketone ester; the elution treatment uses ethanol aqueous solutions with increasing concentrations in sequence. The method provided by the application can effectively improve the extraction yield of ginkgo leaf ketone ester, and the antioxidant effect of ginkgo leaf ketone ester obtained by using the method is greatly improved.

The invention discloses a preparation method of cough and asthma relieving granules, and belongs to the field of traditional Chinese medicine preparation, and the preparation method comprises the following steps: S1, sealed extraction to obtain a mixed solution: performing pulse type ultrasonic treatment on folium rhododendri daurici in an ethanol water solution for 1-3 minutes, then extracting for 66-78 hours at the temperature of 30-40 DEG C, and intermittently stirring; s2, filtering and squeezing to obtain an extracting solution; s3, concentrating the extracting solution to obtain a clear paste with the relative density of 1.10-1.2 at the temperature of 70-80 DEG C; s4, adjusting the pH value to 3.5-5.5, and removing astringency; s5, filtering with an ultrafiltration membrane, concentrating to obtain clear paste with the relative density of 1.10-1.2 at 70-80 DEG C, and spray-drying the clear paste to obtain extract powder; and S6, adding auxiliary materials into the extract powder, mixing, adjusting, preparing granules, drying, finishing the granules, screening, totally mixing, and sub-packaging to obtain the cough and asthma relieving granules. The extraction time is shortened, and the product stability is improved.

The application relates to the technical field of concrete, and discloses high-permeability-resistant recycled micro-powder concrete and a preparation method thereof. The steps are as follows: 3-(isobutenoyloxy)propyl trimethoxysilane is taken, ethanol aqueous solution is added, the temperature is increased to 40-60 DEG C, and stirring is carried out for 1-2 hours; nano glass beads are added, and the temperature is continuously kept and stirred for 2-4 hours; filtration, washing and drying are carried out, and double-bonded glass beads are obtained; iso-pentenyl polyoxyethylene ether is taken, water is added, and stirring is carried out until the water is uniformly mixed; an initiator is added, and stirring and dissolution are carried out, so that a mixed solution A is obtained; acrylic acid, sodium methallyl sulfonate and double-bonded glass beads are taken, water is added, and stirring is carried out until the water is uniformly mixed; the temperature is increased to 60-70 DEG C, the mixed solution A is added dropwise, the dropwise addition is completed in 2-3 hours, the temperature is increased to 75-80 DEG C, and the temperature is kept for 3-4 hours; the pH value is adjusted to be neutral; filtration and drying are carried out, and modified micro-beads are obtained; coarse aggregate, fine aggregate, recycled micro-powder, fly ash, cement, modified micro-beads, an additive and water are mixed, and high-permeability-resistant recycled micro-powder concrete is obtained.

The invention relates to the technical field of concrete, and discloses high-impermeability regenerated micro-powder concrete and a preparation method thereof. The preparation method comprises the following steps: adding 3-(methacryloyloxy) propyltrimethoxysilane into an ethanol water solution, heating to 40-60 DEG C, stirring for 1-2 hours, adding nano glass beads, continuously carrying out heat preservation and stirring for 2-4 hours, filtering, washing and drying to obtain double-bond glass beads; the preparation method comprises the following steps: taking isopentenyl polyoxyethylene ether, adding the isopentenyl polyoxyethylene ether into water, uniformly stirring, adding an initiator, and stirring and dissolving to obtain a mixed solution A; adding acrylic acid, sodium methallyl sulfonate and double-bond glass beads into water, uniformly stirring, heating to 60-70 DEG C, dropwise adding the mixed solution A for 2-3 hours, heating to 75-80 DEG C, keeping the temperature for 3-4 hours, adjusting the pH value to be neutral, filtering, and drying to obtain modified microspheres; and mixing the coarse aggregate, the fine aggregate, the regenerated micro powder, fly ash, cement, the modified microbeads, an additive and water to obtain the high-impermeability regenerated micro powder concrete.

One embodiment of the present invention provides a method of producing a fibrous non-demineralized bone graft material, the method comprising: a step of removing soft tissue, blood, and bone marrow from bone; a dehydration step of immersing, in an aqueous ethanol solution, the bone from which the soft tissue, blood, and bone marrow have been removed, and stirring same; a primary defatting step of immersing and stirring the dehydrated bone in diethyl ether; a step of crushing the bone subjected to the primary defatting into fibrously processed bone; a step of screening the fibrously processed bone; a hydration step of immersing and stirring the screened fibrously processed bone in ethanol; a secondary defatting step of immersing and stirring the hydrated fibrously processed bone in hydrogen peroxide; a washing step of washing the fibrously processed bone subjected to the secondary defatting; and a freeze-drying step.

The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a raw material mixing step for mixing ground pine needles, ground pine cones, heartleaf houttuynia herbs, green tea leaves, cotyledons and an ethanol aqueous solution, and to a method for producing a composition for promoting hair growth. A fermentation step: inoculating the mixture cured in the curing step with lactic acid bacteria and fermenting; and mixing the additives, namely mixing the additives containing the hair root cell growth factors. A fermentation product produced by the fermentation step. The composition for promoting hair growth prepared by the method provides nutrition for hair, improves the health of scalp and hair roots, and shows an excellent hair growth effect.

This invention discloses a Jacaranda flower extract, its preparation method, and its applications, belonging to the field of pharmaceutical technology. The preparation method includes: drying and pulverizing Jacaranda flowers, extracting them by heating and reflux with an ethanol-water solution, concentrating the extract under reduced pressure to obtain a first crude extract; mixing the first crude extract with an aqueous two-phase mixture for extraction, concentrating the upper phase under reduced pressure to obtain a second crude extract; purifying and eluting the second crude extract using a macroporous resin, decolorizing the eluent, and drying to obtain the Jacaranda flower extract. The Jacaranda flower extract obtained by this method has clearly defined active ingredients, exhibits significant photoprotective effects against UVB, and demonstrates significant repair effects after UVB photodamage. It is highly safe and can be used as an active ingredient in skincare products for sun protection.

A high specific surface area mesoporous alumina material with a surface rich in hydroxyl groups is produced by dissolving an aluminum source in an anhydrous organic alcohol solvent containing organic carboxylic acids, slowly and uniformly adding an aqueous ethanol solution to form an alumina sol, drying to obtain an alumina gel, and then treating with high-temperature steam at 400–650°C to obtain a material with... c Mesoporous alumina materials with Al2O3 crystal phase structure, well-developed mesoporous channel structure, high specific surface area and pore volume, and containing 5.0-10.0 mmol / g aluminum hydroxyl groups on the surface of mesoporous pore walls are suitable as catalyst supports for dehydrogenation reactions. The highly uniform loading of metal Pt active components significantly improves the low-temperature reaction activity, selectivity and stability of the catalyst for efficient dehydrogenation of liquid organic hydrogen supports.

This invention belongs to the field of curing agent technology, specifically relating to a composite curing agent, its preparation method, and its application. The composite curing agent comprises the following raw materials in parts by weight: 40-50 parts crushed stone, 20-30 parts cement, 5-10 parts quicklime, 4-10 parts activator, 3-8 parts modified bentonite, and 0.3-0.5 parts crack-resistant agent. The preparation process of the modified bentonite is as follows: (1) Pretreated bentonite is added to an ethanol aqueous solution, then KH570 is added, and the mixture is stirred under heating conditions to obtain pre-modified bentonite; (2) The pre-modified bentonite is added to N,N-dimethylformamide, then diallyl malate and an initiator are added, and the mixture is heated to obtain the modified bentonite. This composite curing agent can significantly improve the mechanical properties and crack resistance of the cured soil.

The invention belongs to the technical field of catalysts, and discloses a mesoporous high-entropy sulfide (CoMnNiZnFe) S2 catalyst, a preparation method and application in preparation of formic acid through electrooxidation of PET hydrolysate, oleic acid is used as a stabilizer, nitrate and urea are mixed in an ethanol aqueous solution, the urea is gradually decomposed in the hydrothermal reaction process to form isocyanic acid and ammonia for controlling the pH value of the solution, and the pH value of the solution is controlled; the method comprises the following steps: reacting metal salt with isocyanic acid to form an M (OH) 2CO3 nano-plate, reacting the metal salt with CO2 released by the isocyanic acid to form the M (OH) 2CO3 nano-plate, calcining the M (OH) 2CO3 nano-plate in air to form a mesoporous high-entropy oxide, and vulcanizing the mesoporous high-entropy oxide to obtain the mesoporous high-entropy sulfide. The electro-catalysis result shows that excellent formic acid selectivity and cycling stability are shown, waste utilization of PET and efficient production of formic acid are achieved, and the application and development requirements of related fields are met.

The method is characterized by comprising the following steps: (1) adding an organic solvent into marine dinoflagellate biomass, and extracting for 1-10 hours in a dark place at 4-40 DEG C; filtering, taking filter residues, repeatedly leaching in a dark place until the leaching solution is colorless, combining the extracting solutions obtained in each time, putting the extracting solutions into a brown bottle, and concentrating at 10-35 DEG C under reduced pressure until the volume is 1 / 10-1 / 2 of the original volume; (2) loading the concentrated solution on a silane bonded silica gel open column, and eluting with an ethanol water solution with the ethanol volume percent of 65-80% to obtain a fraction rich in the polyrocoxanthin; and (3) adding pure water into the fraction rich in the polyrocoxanthin until the alcohol content of the solution is 20-60% by volume, then placing the solution in a refrigerator at-20 DEG C, freezing and standing for 2-8 days in a dark place, and carrying out solid-liquid separation to obtain a high-purity polyrocoxanthin product with the purity of more than 90%, and the method has the advantages of simplicity and rapidness in operation, high extraction purity and high recovery rate.

The invention provides a basil seed total polyphenol extraction method and a basil seed total polyphenol extract.The extraction method comprises the following steps that a basil seed raw material is subjected to degreasing treatment through a degreasing solvent, and a degreased raw material is prepared; carrying out ultrasonic extraction on the degreased raw material by adopting an ethanol water solution to obtain basil seed total polyphenol; wherein the degreasing solvent comprises petroleum ether; the volume ratio of ethanol in the ethanol aqueous solution is 40-80%; based on g / mL, the material-liquid ratio of the degreased raw material to the ethanol water solution is 1: (20-70); the ultrasonic extraction time is 20 to 70 minutes. According to the extraction method, the extraction efficiency of the basil seed total polyphenol can be improved, and damage to polyphenol activity is reduced.

The application discloses a method for separating and purifying ginsenoside F2 from fermentation liquor, and belongs to the technical field of separation and purification. The method comprises the following steps: taking the fermentation liquor from a tank, centrifuging, taking the lower precipitate, adding ethanol to stir and extract, filtering after extraction, and spin-drying the filtrate to obtain a crude product; then dissolving the crude product in an ethanol aqueous solution with a volume fraction of 10-40% to form a sample solution; then performing sample loading on YLT810 resin, and after sample loading is completed, washing impurities with an ethanol aqueous solution with a volume fraction of 45-50%, and when the liquid-phase purity of the target product reaches 70-75%, eluting with an ethanol aqueous solution with a volume fraction of 60-65%, collecting the eluate, and spin-drying to collect the solid, so that ginsenoside F2 is obtained. The method can obtain ginsenoside F2 with a purity of more than 85%, and the yield can reach more than 80%, and is suitable for industrial production.