Method for producing composition for promoting hair growth

Abstract

The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a raw material mixing step for mixing ground pine needles, ground pine cones, heartleaf houttuynia herbs, green tea leaves, cotyledons and an ethanol aqueous solution, and to a method for producing a composition for promoting hair growth. A fermentation step: inoculating the mixture cured in the curing step with lactic acid bacteria and fermenting; and mixing the additives, namely mixing the additives containing the hair root cell growth factors. A fermentation product produced by the fermentation step. The composition for promoting hair growth prepared by the method provides nutrition for hair, improves the health of scalp and hair roots, and shows an excellent hair growth effect.

Description

TECHNICAL FIELD

[0001] The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a method for producing a composition for promoting hair growth, which provides nutrition to hair and has an excellent hair growth promotion effect by improving the health of a scalp and hair roots. BACKGROUND

[0002] A human body has 100,000 to 150,000 hairs, each of which has a different cycle, goes through the steps of anagen, catagen, and telogen, and repeats this cycle for 3 to 6 years. On average, 50 to 100 hairs are shed per day.

[0003] In general, alopecia refers to a cause of an abnormal increase in the number of hairs shed due to a decrease in the proportion of growing hair and an increase in the number of hair in the catagen or telogen phase in this cycle. Male hormone over-secretion, seborrhea, stress, obesity, peroxide, and deterioration of scalp function caused by bacteria are suspected to be the main causes, but it has recently been found to be caused by genetic factors. Hormonal changes, psychological stress, and drug side effects cause hair follicles (HF) to cause changes in the levels of surrounding growth factors, cytokines, and hormones, resulting in alopecia.

[0004] The present inventors have been developing a natural hair restorer and have continuously researched the causes, symptoms, prescriptions, and treatment methods of alopecia, and pine needle extract and pine drop extract are widely used to maintain blood sugar levels or as a cosmetic raw material, and by confirming that 3-carene, limonene, and terpinolene, which are effective in promoting hair growth, are contained as main components, the present invention has been completed.

[0005] PRIOR ART DOCUMENT

[0006] PATENT DOCUMENT

[0007] Patent Document 1: Korean Patent Registration No. 10-0992565 (2010.11.01.)

[0008] Patent Document 2: Korean Patent Registration No. 10-2074314 (2020.01.31.) SUMMARY

[0009] PROBLEMS TO BE SOLVED BY THE INVENTION

[0010] The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a method for producing a composition for promoting hair growth, which provides nutrition to hair and has an excellent hair growth promotion effect by improving the health of a scalp and hair roots.

[0011] MEANS FOR SOLVING THE PROBLEMS

[0012] The present invention is to produce 100 parts by weight of pine needle powder, 80 to 120 parts by weight of pine cone powder, 80 to 120 parts by weight of Houttuynia cordata, 40 to 60 parts by weight of green tea leaves, 40 to 60 parts by weight of cotyledon, and 500 to 1000 parts by weight of an aqueous ethanol solution. A raw material mixing step, in which each part is mixed; a maturation step, in which the mixture prepared through the raw material mixing step is aged for 5 to 10 days at a temperature of 8 to 12°C, low-temperature ripening for 4 to 6°C, and low-temperature maturation for 20 to 30 days; and a fermentation step, in which 0.01 to 0.1 parts by weight of lactic acid bacteria are inoculated into 100 parts by weight of the mixture, are provided. A fermentation product of 100 parts by weight produced through the maturation step and the fermentation step at a temperature of 30 to 35°C for 2 to 4 days, and a hair growth promoting composition are provided by providing a method of producing a composition for promoting hair growth, which includes an additive mixing step of mixing 0.1 to 1 parts by weight of an additive containing a hair root cell growth factor.

[0013] According to a preferred feature of the present invention, the pulverized pine needles and pine cones are manufactured by putting the multi-stem pine needles and pine cones into a pulverizer and pulverizing them to a particle size of 0.01 to 0.1 mm.

[0014] According to a more preferred feature of the present invention, the lactic acid bacteria consist of one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus coryniformis, Lactobacillus plantarum, and Leuconostoc lactis.

[0015] According to a more preferred feature of the present invention, the additive contains 1 to 10 parts by weight of a hair root cell growth factor per 100 parts by weight of an aromatic oil, and the hair root cell growth factor is sh-polypeptide-10.

[0016] According to a more preferred feature of the present invention, the aromatic oil is made of one or more selected from the group consisting of jasmine oil, peppermint oil, lavender oil, rosemary oil, eucalyptus oil, tea tree oil, and palmarosa oil.

[0017] Inventive Effects

[0018] The method for producing a composition for promoting hair growth according to the present invention has an excellent effect of providing a composition for promoting hair growth, which supplies nutrients to hair, improves the health of a scalp and a hair root, and exhibits an excellent hair growth promotion effect. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 is a sequence diagram of the method of manufacturing the composition for promoting hair growth according to the present invention.

[0020] REFERENCE NUMERALS

[0021] S101: a raw material mixing step;

[0022] S103: a maturation step;

[0023] S105: a fermentation step;

[0024] S107: an additive mixing step. DETAILED DESCRIPTION

[0025] The present application can be easily implemented by those skilled in the art. The technical idea and scope of the present application are limited.

[0026] The method for producing a composition for promoting hair growth according to the present application includes a raw material mixing step S101 of mixing crushed pine needles, crushed pine cones, houttuynia cordata, green tea leaves, cotyledons, and an ethanol aqueous solution, a maturation step S103 of maturing the prepared mixture through the raw material mixing step S101, a fermentation step S105 of inoculating lactic acid bacteria and fermenting into the mixture matured through the maturation step S103, and mycorrhizae. The fermentation product produced through the fermentation step S105 includes an additive mixing step S107 of mixing an additive containing a cell growth factor therein.

[0027] The raw material mixing system S101 is a step of mixing pine needle powder, pine cone powder, houttuynia cordata, green tea leaves, cotyledons, and an ethanol aqueous solution, in which 100 parts by weight of pine needle powder, 80 to 120 parts by weight of water powder. Preferably, it is made by mixing 80 parts by weight of crushed pine balls, 80 parts by weight of houttuynia cordata, 40 to 60 parts by weight of green tea leaves, 40 to 60 parts by weight of cotyledons, and 500 to 1,000 parts by weight of an ethanol aqueous solution.

[0028] The above crushed pine needles and crushed pine cones can be used as pine needles and pine cones obtained from Korean Pinus densiflora, Pinus densata, Pinus koraiensis, Pinus strobus, Pinus parviflora, Pinus bungeana, Pinus thunbergii, and Pinus massoniana, but Korean Pinus densiflora is preferably used, and they are manufactured by putting them into a grinder and crushing them to a particle size of 0.01 to 0.1 mm.

[0029] The crushed pine needles and pine cones crushed to the above particle size have a larger specific surface area in contact with the ethanol aqueous solution, and can improve the extraction efficiency of 3-carene, limonene, and pinene components.

[0030] The main component of the pine needle and pine cone terpene oil includes α-pinene, camphene, β-pinene, β-myrcene, and β-phellandrene, and it not only promotes blood circulation of the scalp by dilating capillaries, but also has an inhibitory effect on various Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa together with another component, chlorophyll. In addition, the unsaturated fatty acid contained in the essential oil has a significant antihypertensive effect by lowering serum cholesterol levels, and has an antibacterial and bactericidal effect on pathogenic fungi causing skin eczema and scabies, thereby inhibiting the occurrence of dandruff. It shows an effect of improving the scalp and reducing itching.

[0031] At this point, it is not preferable if the particle size of pine needle powder and pine cone powder is less than 0.01 mm, because if the particle size is less than 0.01 mm, it is easy to scatter or the particles may agglomerate. If the particle size exceeds 0.1 mm, this is also undesirable, as it may reduce the aforementioned effects.

[0032] Houttuynia cordata contains 80-120 parts by weight and has the effect of protecting hair and scalp. Houttuynia cordata is a perennial herb of the Saururaceae family, with detoxifying and diuretic effects, and can relieve symptoms of whooping cough, bronchitis, and hepatitis. Quercetin is the main physiologically active substance of Houttuynia cordata, with cardiotonic effects, sustained vasoconstrictive effects, antibacterial effects against various intestinal bacteria and filamentous fungi, antiviral effects, natural killer cell activity, enhanced immune function, and anti-pneumonia induction effects. It has also been reported to have anti-tumor effects. The general composition of Houttuynia cordata leaves is 10.5% moisture, 12.5% ​​crude protein, 4.0% crude fat, 13.0% ash, and 13.8% crude fiber. Studies have found that the stems have low crude protein and crude fat content, but high fiber content, and are also high in essential amino acids and unsaturated fatty acids. Furthermore, it has been reported that Houttuynia cordata extract exhibits high antioxidant properties and strong inhibitory effects against influenza, herpes simplex virus (HSV-1), and HIV-1, but does not show cytotoxicity. Furthermore, the main components of houttuynia cordata essential oil, such as methyl N-nonyl ketone, lauraldehyde, and octanal, have been shown to inhibit the activity of these viruses. It can effectively prevent suppuration and fever.

[0033] Furthermore, if the content of houttuynia cordata is less than 80 parts by weight, the above effects are negligible; if the content of houttuynia cordata exceeds 120 parts by weight, the above effects are not significantly improved, but the content of other active ingredients will decrease. Excessive reduction in ingredients is undesirable.

[0034] In addition, green tea contains 40 to 60 parts by weight of substances that not only prevent dandruff and odor through antibacterial effects, but also contains a component called catechin extracted from green tea leaves, which has anti-androgenic effects and prevents hair loss by inhibiting DHT hormone, a hormone that causes hair loss.

[0035] The effect is minimal if the green tea content is less than 40 parts by weight, and the effect will not be significantly improved if the green tea content exceeds 60 parts by weight, and the manufacturing cost may increase excessively, which is not advisable.

[0036] Additionally, a cotyledon content of 40 to 60 parts by weight strengthens hair and improves scalp health. The effect is minimal if the cotyledon content is below 40 parts by weight, with a maximum of 60 parts by weight. Exceeding this weight limit will not significantly enhance the aforementioned effects, and the content of other active ingredients will be relatively reduced, which is undesirable.

[0037] Additionally, the ethanol-water solution contains 500-1000 parts by weight, preferably at a concentration of 40-60%. If the concentration of the ethanol-water solution is less than 40%, crushed pine needles or crushed pine cones are used. The efficacy of the Houttuynia cordata hair growth composition prepared according to the present invention may be reduced due to the decreased elution of active ingredients contained in green tea leaves and cotyledons, and if the concentration of ethanol in the aqueous solution exceeds 60%, this is undesirable, as the skin toxicity of the hair growth promoting composition prepared by this method may be excessively increased.

[0038] The maturation step S103 is a step of maturing the mixture prepared by the raw material mixing step S101, and the mixture prepared by the raw material mixing step S101 is preferably maturated at a temperature of 8 to 12°C for 5 to 10 days, or at a low temperature of 4 to 6°C for 20 to 30 days.

[0039] When the ripening process is carried out normally and at low temperature as described above, not only are a large number of active ingredients contained in pine needles, pine cones, houttuynia cordata, green tea leaves, and cotyledons washed out, but they also emit an unusual odor. (This process reduces the amount of active ingredients.)

[0040] Fermentation step S105 involves inoculating the mixture after maturation step S103 with lactic acid bacteria and fermenting it. The amount of lactic acid bacteria inoculated is 0.01 to 0.1 parts by weight per 100 parts by weight of the mixture after maturation step S103. Maturation step S103 is performed at a temperature of 30 to 35°C for 2 to 4 days.

[0041] After the fermentation step S105, which consists of the above-described process, not only is the amount of active ingredients eluted from the mixture aged in the maturation step S103 increased, but the eluted active ingredients are also formed into easily separable particles. After being absorbed into the body, these particles can be broken down to reveal larger sizes, thereby further enhancing their efficacy in promoting hair growth.

[0042] If the inoculation amount of lactic acid bacteria is less than 0.01 parts by weight, the fermentation step S105 may not be carried out properly or may be carried out too slowly, thereby reducing the efficiency of the manufacturing process. If the inoculation amount of lactic acid bacteria exceeds 0.1 parts by weight, the fermentation step S105 is undesirable because it may excessively increase the manufacturing cost without improving efficiency.

[0043] Preferably, the composition is selected from one or more of *Lactobacillus paracasei*, *Lactobacillus rhamnosus*, *Lactobacillus curvatureus*, *Lactobacillus plantarum*, and *Leuconostoc mesenteroides*. In particular, *Lactobacillus paracasei* not only exhibits the aforementioned effects but also promotes the proliferation of animal connective tissue—human hair follicle dermal papilla cells (cells constituting the hair root) and fibroblasts (NIH3T3)—thus promoting hair growth. Since it itself exhibits a hair growth-promoting effect by increasing the expression of genes related to growth and the cell growth cycle, the hair growth-promoting effect of the hair growth-promoting composition prepared by this invention can be further enhanced.

[0044] Furthermore, fermentation will not proceed normally if the temperature in fermentation step S105 is below 30°C or the fermentation time is less than 2 days. Similarly, fermentation will not proceed normally if the temperature in fermentation step S105 exceeds 35°C or the fermentation time is less than 2 days. If the fermentation time exceeds 4 days, over-fermentation may occur, resulting in an excessive increase in fermentation odor, or anaerobic fermentation may occur in the mixture after maturation step S103, leading to spoilage, which is undesirable.

[0045] The additive mixing step S107 involves mixing 100 parts by weight of the additive containing hairy root cell growth factor with the fermentation product prepared by fermentation step S105. Preferably, 0.1 to 1 part by weight of the additive containing hairy root cell growth factor is mixed.

[0046] At this point, the additive containing hair follicle growth factor consists of 1 to 10 parts by weight of hair follicle growth factor per 100 parts by weight of aromatic oil. The additive composed of the above ingredients improves scalp health and hair roots, making them thicker and shinier, thus promoting hair growth.

[0047] At this time, the aromatic oil is preferably composed of one or more ingredients selected from jasmine oil, peppermint oil, lavender oil, rosemary oil, eucalyptus oil, tea tree oil, and palmarosa oil. Used to remove sebum from the scalp, it not only removes and relieves skin inflammation, but also improves scalp health by providing moisturizing and a refreshing feeling.

[0048] In addition, hair follicle growth factor (HGF) is composed of sh-peptide-10 (KGF-2), which promotes the growth of hair follicle cells, thereby providing thicker, shinier hair. The concentration of HGF is 1 part by weight. If the concentration is less than 10 parts by weight, the effect is minimal, while if the concentration exceeds 10 parts by weight, the effect will not be significantly improved, and the manufacturing cost may increase excessively, which is undesirable.

[0049] After the above-described additive mixing step S107, the preparation of the hair growth promoting composition according to the present invention is completed. The prepared hair growth promoting composition can be used directly, but it is convenient to use. Considering ease of use, it is preferable to use it after removing the solid components by filtration.

[0050] At this point, there are no particular restrictions on the filtration method or device used, as long as it can physically remove the solids contained in the hair growth-promoting composition. A general-purpose filtration device is recommended. A separatory funnel is also used to prevent oxidation.

[0051] The following examples illustrate the method for manufacturing the hair growth promoting composition of the present invention and the physical properties of the hair growth promoting composition prepared by the method.

[0052] <Preparation Example 1> Preparation of Crushed Pine Needles

[0053] Joseon Forma multicaulis pine needles were crushed in a pulverizer to prepare crushed pine needles with a particle size of 0.05 mm.

[0054] <Preparation Example 2> Preparation of Crushed Pine Cones

[0055] North Korean pine cones were crushed in a pulverizer to produce pulverized pine cones with a particle size of 0.05 mm.

[0056] <Preparation Example 3> Preparation of an Additive Containing Hair Root Cell Growth Factor

[0057] An additive containing hair root cell growth factor was prepared by mixing 100 parts by weight of aromatic oil (rose geranium oil) with 5 parts by weight of hair root cell growth factor (sh-peptide-10).

[0058] <Example 1>

[0059] 100 parts by weight of pine needle powder prepared in Preparation Example 1, 100 parts by weight of pine cone powder prepared in Preparation Example 2, 100 parts by weight of houttuynia cordata, and 50 parts by weight of green tea leaves were added to a mixing device equipped with a stirrer with a mass concentration of 750 parts by weight of 50% ethanol aqueous solution. The mixture was stirred at 200 rpm for 10 minutes and aged at 60°C. After incubation at 10°C for 8 days, and then stirring at 5°C for 25 days of low-temperature aging, 0.05 parts by weight of lactic acid bacteria (Lactobacillus paracasei) were inoculated into 100 parts. The aged mixture was fermented at 33°C for 3 days, and 100 parts by weight of the fermentation product were mixed with 0.5 parts by weight of an additive containing hair root cells to prepare a composition for promoting hair growth. The growth factor prepared in Preparation Example 3 was also included.

[0060] <Example 2>

[0061] Similar to Example 1, but 80 parts by weight of pine cone powder, 80 parts by weight of houttuynia cordata, 40 parts by weight of green tea leaves, and 40 parts by weight of cotyledons prepared in Preparation Example 2 were mixed and then processed as in Preparation Example 3. A composition for promoting hair growth was prepared by mixing 0.1 parts by weight of an additive containing the prepared hair root cell growth factor.

[0062] <Example 3>

[0063] The procedure was carried out in accordance with Example 1, except that 120 parts by weight of pine cone powder, 120 parts by weight of houttuynia cordata, 60 parts by weight of green tea leaves, and 60 parts by weight of cotyledons prepared in Example 2 were mixed with 1 part by weight of an additive containing the prepared hair root cell growth factor to prepare a composition for promoting hair growth.

[0064] <Example 4>

[0065] The composition used to promote hair growth differs in that it is aged at 8°C for 5 days and then at a low temperature of 4°C for 20 days.

[0066] <Example 5>

[0067] The composition for promoting hair growth was prepared in the same manner as in Example 1 by aging at 12°C for 10 days and at a low temperature of 6°C for 30 days.

[0068] <Example 6>

[0069] The composition for promoting hair growth was prepared in the same manner as in Example 1, but fermented at 30°C for 2 days.

[0070] <Example 7>

[0071] The same procedure as in Example 1 was performed, but fermentation was carried out at 35°C for 4 days to prepare a composition for promoting hair growth.

[0072] <Comparative Example 1>

[0073] 100 parts by weight of pine needles, 100 parts by weight of pine cones, 100 parts by weight of houttuynia cordata, 50 parts by weight of green tea leaves, 50 parts by weight of cotyledons, and 750 parts by weight of an aqueous ethanol solution were added to a mixing apparatus to prepare a 50% (w / w) mixture. The mixture was stirred at 200 rpm for 10 minutes to prepare a hair growth promoting composition. The prepared mixture was then aged at a certain temperature. It was kept at 10°C for 10 days to prepare a hair growth promoting composition.

[0074] <Comparative Example 2>

[0075] The procedure was the same as in Example 1, but the hair growth-promoting composition was prepared by mixing 50 parts by weight of ground pine cones, 50 parts by weight of houttuynia cordata, 20 parts by weight of green tea leaves, and 20 parts by weight of a hair growth agent. The weight of the cotyledons obtained in Example 2 was used.

[0076] <Comparative Example 3>

[0077] The composition for promoting hair growth was prepared in the same manner as in Example 1, but aged at 10°C for 10 days.

[0078] <Comparative Example 4>

[0079] The hair growth promoting composition was prepared in the same manner as in Example 1, but fermented at 20°C for 3 days.

[0080] <Comparative Example 5>

[0081] The composition for promoting hair growth was prepared in the same manner as in Example 1, but without mixing the additive containing hair root cell growth factor prepared in Preparation Example 3.

[0082] The hair growth promoting effects of the hair growth promoting compositions prepared by Examples 1 to 7 and Comparative Examples 1 to 5 were measured and are shown in Table 1 below.

[0083] However, the hair growth promoting effect was determined by measuring the proliferation rate of hair papilla cells in the prepared hair growth promoting composition.

[0084] The proliferation rate of dermal papilla cells was measured using an MTT assay with human hair follicle dermal papilla cells. Specifically, after removing the culture medium from the dermal papilla cells cultured in a T75 flask, 3 mL of PBS was added to incubate the cells. The cells were gently shaken to prevent spillage, and then washed with water. After removing the PBS, 2 mL of trypsin-EDTA solution was added and incubated for 5 minutes under a 5% CO2 atmosphere. 8 mL of DMEM medium was added to the cultured cells to detach them from the surface, transfer them to Falcon tubes, remove the medium using a centrifuge (M15R, Hanil, Korea) at 5°C and 1,000 rpm, and 10 mL of 10% FBSDMEM medium was added to a Voltexer to resuspend them. Cells were then counted at 1 × 10⁻⁶ cells per ... 4 1 cell / well was seeded with suspension cells and cultured in a CO2 incubator for 24 hours.

[0085] As a control group, triple-distilled water with a concentration of less than 1 μS / cm was used to filter the hair growth promoting compositions prepared in Examples 1 to 7 and Comparative Examples 1 to 5 through a 0.2 μm filter. After treating the cells with 0.01% of the filtrate, the cells were cultured for 24 hours using a substance to remove solids.

[0086] Cells were incubated in a CO2 incubator using cooking foil for 4 hours, then the culture medium was removed and DMSO was added to form colored formazan for 30 minutes. Finally, the proliferation rate of dermal papillary cells was measured by measuring the OD absorbance at 570 nm using a microplate reader (EPOCH, BIOTEK, USA).

[0087]

[0088] Distinguish Proliferation rate (%) of dermal papilla cells Control group 100 Example 1 172.5±5.4 Example 2 168.3±4.7 Example 3 179.1±4.9 Example 4 171.0±5.9 Example 5 175.6±4.8 Example 6 171.8±4.2 Example 7 173.9±4.8 Comparative Example 1 142.2±5.2 Comparative Example 2 156.2±4.9 Comparative Example 3 166.7±4.5 Comparative Example 4 162.9±4.8 Comparative Example 5 159.1±5.1

[0089] As shown in Table 1, it can be seen that the hair growth promoting compositions prepared by Examples 1 to 7 of the present invention have excellent hair papilla cell proliferation rates compared with the hair growth promoting compositions prepared by Comparative Examples 1 to 7. Therefore, the hair growth promoting compositions prepared by Examples 1 to 7 of the present invention exhibit excellent hair growth effects.

[0090] In addition, the hair growth and hair growth effect of the compositions for promoting hair growth prepared by Examples 1 to 7 and Comparative Examples 1 to 5 were measured and are shown in Table 2 below.

[0091] However, the hair growth effect was obtained by filtering the hair growth-promoting compositions prepared in Examples 1 to 7 and Comparative Examples 1 to 5 to remove solid contents, and using the filtrate with the solid contents removed. In a study involving a total of 50 men and women experiencing hair loss symptoms with thinning and fragile hair, 10 ml was applied to the scalp once daily for 3 months, and hair thickness and density were compared. Measurements were performed using a 5-point scale, and the average value was used to represent the results.

[0092] 5 points: Very improved, 4 points: Improved, 3 points: No improvement, 2 points: Worsened, 1 point: Very worsened

[0093]

[0094] Distinguish Thickness of hair Density of hair Example 1 4.3 4.4 Example 2 4.2 4.2 Example 3 4.4 4.5 Example 4 4.3 4.4 Example 5 4.4 4.4 Example 6 4.2 4.4 Example 7 4.4 4.5 Comparative Example 1 3.7 3.6 Comparative Example 2 3.8 4.0 Comparative Example 3 4.1 4.1 Comparative Example 4 3.9 3.9 Comparative Example 5 3.8 4.1

[0095] As shown in Table 2, it can be seen that the hair growth promoting compositions prepared by Examples 1 to 7 of the present invention exhibit superior hair growth effects compared with the hair growth promoting compositions prepared by Comparative Examples 1 to 5.

[0096] Therefore, the method for preparing a hair growth promoting composition according to the present invention provides a hair growth promoting composition that supplies nutrients to hair, improves the health of the scalp and hair roots, and exhibits excellent hair growth promoting effect.

Claims

1. A method of manufacturing a hair growth promoting composition, characterized in that, the method of manufacturing the hair growth promoting composition comprises: a raw material mixing step of mixing 100 parts by weight of pine needle powder, 80 to 120 parts by weight of pine cone powder, 80 to 120 parts by weight of Houttuynia cordata, 40 to 60 parts by weight of green tea leaves, 40 to 60 parts by weight of cotyledon, 40 to 60 parts by weight of cypress fruit, and 500 to 1000 parts by weight of an aqueous ethanol solution; a maturation step of maturing the mixture prepared in the raw material mixing step at a temperature of 8 to 12°C for 5 to 10 days and low-temperature maturing at a temperature of 4 to 6°C for 20 to 30 days; a fermentation step of inoculating 0.01 to 0.1 parts by weight of lactic acid bacteria into 100 parts by weight of the mixture after the maturation step and fermenting at a temperature of 30 to 35°C for 2 to 4 days; and an additive mixing step of mixing 0.1 to 1 parts by weight of an additive containing a hair root cell growth factor with 100 parts by weight of the fermentation product prepared in the fermentation step.

2. The method of manufacturing the hair growth promoting composition according to claim 1, characterized in that, the pine needle and the pine cone are pulverized by putting the pine needle and the pine cone into a grinder and pulverizing to a particle size of 0.01 to 0.1 mm.

3. The method of manufacturing the hair growth promoting composition according to claim 1, characterized in that, the lactic acid bacteria is one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus curvatus, Lactobacillus plantarum, and Leuconostoc lactis.

4. The method of manufacturing the hair growth promoting composition according to claim 1, characterized in that, the additive is composed of 1 to 10 parts by weight of a hair root cell growth factor per 100 parts by weight of an aromatic oil, the hair root cell growth factor is sh-polypeptide-10.

5. The method of manufacturing the hair growth promoting composition according to claim 4, characterized in that, the aromatic oil is composed of one or more selected from the group consisting of jasmine oil, peppermint oil, lavender oil, rosemary oil, eucalyptus oil, tea tree oil, and palmarosa oil.

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