3132 results about "Germplasm" patented technology

The invention relates to the technical field of breeding planning, in particular to an intelligent breeding planning and decision-making method and system based on a large model. The method comprises the following steps: acquiring a multi-source breeding data set; constructing a structured breeding knowledge graph based on the multi-source breeding data set; performing breeding data association on the structured breeding knowledge graph according to a preset large model to generate a special breeding basic model; obtaining a breeding instruction input by a user; performing user semantic recognition on a breeding instruction input by a user to generate breeding semantic recognition data; inputting the breeding semantic recognition data into a breeding special basic model for breeding intention analysis, and generating user breeding intention data; and determining data information needing to be called based on the breeding intention data of the user, analyzing and screening to generate germplasm resource screening data and a breeding plan / breeding decision scheme. According to the method, the intelligence and operability of breeding planning are improved through integration of multi-source data, intelligent semantic recognition, combined genetic analysis and executable evaluation.

The present invention relates to the field of tobacco breeding, and more particularly to molecular markers for screening for spotted wilt-resistant tobacco plants free of linkage drag and their applications. A method for screening spotted wilt-resistant tobacco plants or germplasm with reduced linkage drag is provided, comprising: (a) screening for spotted wilt-resistant tobacco plants or germplasm and isolating nucleic acid thereof; (b) detecting a first linkage drag locus marker and / or a second linkage drag locus marker in the isolated nucleic acid; the first linkage drag locus marker comprising the NaChr4_2M marker shown in SEQ ID No. 10 and / or the NaChr4_8M marker shown in SEQ ID No. 11; the second linkage drag locus marker comprising the NaChr3_62.6M marker shown in SEQ ID No. 7 and / or the NaChr3_64.6M marker shown in SEQ ID No. 8; and (c) selecting a spotted wilt-resistant tobacco plant or germplasm that does not contain the first linkage drag locus marker and / or the second linkage drag locus marker. This method can be used to obtain spotted wilt-resistant tobacco plants free of linkage drag, providing superior varieties with high spotted wilt resistance for tobacco leaf production.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention discloses an AsMYB19 gene and application thereof, and relates to the technical field of biology, the nucleotide sequence of the AsMYB19 gene is shown as SEQ ID NO.1, the AsMYB19 gene can regulate and control the tiller number and the yield of oat, gene resources and strategies are provided for oat breeding and improvement of oat germplasm resources, the breeding speed is increased, and the breeding efficiency is improved.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention discloses an AsSN1 gene and application, and relates to the technical field of biology. The nucleotide sequence of the AsSN1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the AsSN1 protein is as shown in SEQ ID NO.2. The AsSN1 gene and the protein provided by the invention can regulate and control the spikelet number of the oats, further regulate and control the yield of the oats, provide important support for oat breeding and germplasm resource improvement, and have important significance for meeting the development requirements of animal husbandry.

The invention discloses a high-yield cold-resistant pennisetum germplasm screening method and application thereof, and the method is based on 10 yield traits, 4 cold-resistant indexes and 4 low-temperature photosynthetic ability indexes of pennisetum, constructs a multi-dimensional dynamic monitoring system across autumn, winter and spring, and performs high-yield cold-resistant comprehensive evaluation on the pennisetum germplasm, thereby screening out a high-yield cold-resistant breeding material. The invention provides a comprehensive, efficient and accurate high-yield cold-resistant Chinese pennisetum germplasm screening method, through a multi-dimensional dynamic monitoring system crossing autumn, winter and spring, yield traits, cold resistance indexes and low-temperature photosynthetic ability indexes are integrated, and the problems that a traditional method is single in evaluation dimension, disjointed with field reality and insufficient in dynamic adaptability are solved. The comprehensive evaluation system based on the principal component quantitative model not only fills the blank of the high-yield and cold-resistant evaluation model of the pennisetum alopecuroides, but also remarkably improves the breeding efficiency, provides powerful support for cultivating cold-resistant and yield-reduction-free breakthrough varieties, and has important application value and popularization prospect.

The invention relates to an artificial hybridization fry breeding method for epinephelus fuscoguttatus and pink pink bass, which belongs to the technical field of distant hybridization breeding and comprises the steps of parent selection of epinephelus fuscoguttatus and pink pink bass, parent breeding, parent intensive breeding, gamete collection, artificial insemination, hatching of fertilized eggs and fry breeding. By means of reproductive regulation and distant hybridization technologies, two species belonging to two genera under the grouper family with different geographical distribution and different breeding time are hybridized, namely cross-generic hybridization under the grouper family is achieved, intergeneric reproductive isolation is broken through, and the breeding efficiency is improved. Therefore, new hybridized germplasm with excellent characteristics of high fertilization rate, high hatching rate, high survival rate, high growth speed and the like is cultivated.

The invention provides an Indel molecular marker primer pair, a kit and a method for identifying Yichang orange, and belongs to the technical field of biochemistry, the Indel molecular marker primer pair comprises at least one of an InDel-2 primer pair and an InDel-3 primer pair; the sequences of the InDel-2 primer pair are as shown in SEQ ID No. 1 and SEQ ID No. 2, and the sequences of the InDel-3 primer pair are as shown in SEQ ID No. 3 and SEQ ID No. 4. The Indel molecular marker primer pair or the kit disclosed by the invention can specifically recognize the Yichang oranges, realizes efficient and accurate identification of the Yichang oranges and filial generations thereof, and has wide application prospects in the aspects of citrus germplasm resource protection, variety right and interest maintenance, breeding selection, market supervision and the like. The method for identifying the Yichang oranges can accurately identify the Yichang oranges and the filial generations of the Yichang oranges, has high specificity, economical efficiency and high efficiency, is an accurate, economical and efficient hybrid identification means, can effectively identify the variety of the Yichang oranges and the hybrids of the Yichang oranges, and provides powerful technical support for scientific research and breeding work.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention discloses a gene and a protein for adjusting tobacco polyphenol aroma substances and application of the gene and the protein. The gene is a tobacco NtMYB28 gene, and the CDS sequence of the tobacco NtMYB28 gene is as shown in SEQ ID No. 1. The invention further discloses application of the tobacco NtMYB28 gene in regulating the content of endogenous polyphenol substances in tobacco and regulating and controlling the metabolic pathway of phenylpropane in tobacco. The key gene NtMYB28 capable of simply and effectively adjusting the content of polyphenol substances in tobacco and the metabolic pathway of phenylpropane is found, and the gene can be applied to efficient cultivation of new high-aroma tobacco materials and development and utilization of new high-value functional tobacco germplasm.

The invention relates to a same-variety grafting method for Tibetan pinus bungeana, and belongs to the technical field of forest vegetative propagation. In order to solve the technical problems that in the high-altitude environment, the grafting survival rate of the white bark pine is low, a connector is prone to being infected by microorganisms, and callus is formed slowly, efficient grafting is achieved through collaborative optimization of scion pretreatment and stock incision treatment. The method comprises the steps that a one-year-old branch with the diameter being 4-6 mm is selected and subjected to temperature and humidity control and composite antibacterial liquid dipping treatment; a sterile healing interface is formed in cooperation with rootstock incision slope cutting and four times of composite antibacterial liquid brushing; a one-year-old branch is cut, at least two or more bud points exist on the branch, two plump bud points are reserved, the branch is cut open from the position between the two plump bud points, and the scion is obtained. According to the method, the grafting survival rate and the interface stress resistance of the pinus bungeana in the alpine region are remarkably improved, the method is suitable for rapid breeding and hereditary characteristic retention of excellent germplasm of the pinus bungeana in Tibet plateau ecological restoration engineering, and more scion resources of the pinus bungeana can be utilized and protected.

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses a tuna pond culture strategy optimization method and system based on meteorological influence analysis, and the method comprises the steps: collecting regional meteorological data and pond culture monitoring data, and constructing a multi-modal pond ecological feature vector; establishing a weather-water body dynamic coupling model by using a space-time diagram neural network, and generating water body situation response prediction information; a breeding regulation and control strategy is made based on the prediction information, and strategy self-adaptive optimization is achieved through real-time monitoring data; evaluating the anti-disaster capability of the tuna after the meteorological influence is finished, screening high-quality germplasm resources by combining genetic analysis, and generating an anti-disaster breeding scheme. According to the method, full-chain optimization from meteorological early warning to culture regulation and control to genetic breeding is achieved, and the climate toughness and economic benefits of tuna pond culture are comprehensively improved.

The invention discloses a tea tree liquid phase chip and application thereof, and relates to the technical field of molecular detection. The invention discloses a tea tree liquid phase chip and application thereof, according to site screening requirements and probe design principles, the tea tree liquid phase chip comprises 5781 SNP sites, and tea tree resource genetic typing can be realized based on a target interval genome sequence liquid phase capture accurate positioning sequencing typing technology. The tea tree liquid chip can realize low-cost genetic typing, is mainly specific to tea trees, can realize variety identification and genetic relationship analysis of the tea trees, scientifically guides hybridization improvement work of the tea trees and assists protection and development of germplasm resources of the tea trees, and has relatively high application values in multiple fields of tea tree breeding. The tea tree liquid phase chip can be used for tea tree genetic diversity evaluation, germplasm resource and genetic relationship identification, genetic map construction and gene localization, whole genome association analysis and tea tree molecular marker assisted breeding.

The invention discloses a silver carp 20K liquid phase chip and application thereof, the liquid phase chip comprises a probe combination of 20,909 SNP molecular markers covering a whole genome, the physical positions of the 20,909 SNP molecular markers are determined based on sequence alignment of the silver carp genome (GCA041475455.1), and the site information of the 20,909 SNP molecular markers is shown in the specification table 1. The invention provides a silver carp first type whole genome SNP liquid phase breeding chip, which effectively reduces the application cost of silver carp in genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research, solves the problem that no applicable product exists in silver carp molecular auxiliary breeding and whole genome selective breeding, accelerates the progress of silver carp basic research and breeding, and has a wide application prospect. The method is of great significance in improving the utilization efficiency of silver carp germplasm resources, accelerating the breeding process of excellent characters and improving the economic value and environmental adaptability of bred varieties.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention provides a method for screening drought-enduring germplasm resources of macadamia nuts, and belongs to the technical field of plant germplasm resource screening. The screening method comprises the following steps: (1) sowing disinfected macadimia nut seeds into a seedling raising tray, and raising seedlings to obtain seedlings; (2) transplanting the seedlings obtained in the step (1) into a nutrient medium, and performing drought stress culture to obtain pretreated seedlings; and (3) determining drought tolerance indexes in the pretreated seedlings obtained in the step (2), and screening to obtain the macadimia nut drought-tolerant germplasm resources. According to the screening method provided by the invention, macadamia nut germplasm resources with relatively strong drought tolerance can be rapidly screened out, and powerful support is provided for cultivation of drought-tolerant varieties.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention discloses development and application of a KASP marker related to wheat freezing resistance, and relates to the technical field of biology, in particular to a method for screening or assisting in screening wheat freezing-resistant germplasm, which comprises the following steps: detecting whether wheat to be detected is genotype qFR-1B. 1a or genotype qFR-1B. 1b, and detecting whether the wheat to be detected is genotype qFR-1B. 1a or qFR-1B. 1b; the freezing resistance of the genotype qFR-1B. 1a wheat is greater than or candidate greater than that of the genotype qFR-1B. 1b wheat; the wheat of which the genotype is qFR-1B. 1a is wheat of which the genotype of a G101A SNP (Single Nucleotide Polymorphism) site is a GG homozygous type; the wheat of which the genotype is qFR-1B. 1b is wheat of which the genotype of a G101A SNP (Single Nucleotide Polymorphism) site is AA homozygous; the G101A SNP site is the 101 nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with relatively high freezing resistance.

The invention relates to the technical field of biological breeding, in particular to a biological breeding management method and system based on big data. The method comprises the following steps: acquiring original germplasm data; multi-source heterogeneous data integration is carried out on the original germplasm data, the data integration comprises genotype sequencing integration and historical phenotype integration, and integrated germplasm data is generated and stored in a database in a distributed mode; obtaining original growth data, performing data preprocessing, generating standardized germplasm resource comprehensive data including a crop image feature set and an environment response parameter set, and synchronizing the data to the database; and performing whole genome selection prediction on the integrated germplasm data based on the standardized germplasm resource comprehensive data to generate a genotype-phenotype prediction model. By integrating germplasm resources, field management, breeding management, phenotype management, genotype management and whole genome selection, the accuracy and adjustment flexibility of biological breeding management are improved.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a KASP molecular marker for identifying soybean protein content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82.a2.v1 genome sequence as a reference genome, is the 42148495th SNP on a soybean chromosome 9 and corresponds to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, and when the locus is TT homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, different protein contents of soybeans are screened or screened in an auxiliary mode, and the different protein contents of the soybeans are as follows: the homozygous soybeans of the genotype A are higher than or candidate to be higher than the homozygous soybeans of the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding offspring materials with high protein content.

A novel soybean variety, designated 5PLSL31 is provided. Also provided are the seeds of soybean variety 5PLSL31, cells from soybean variety 5PLSL31, plants of soybean 5PLSL31, and plant parts of soybean variety 5PLSL31. Methods provided include producing a soybean plant by crossing soybean variety 5PLSL31 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and / or a native trait into soybean variety 5PLSL31, methods for producing other soybean varieties or plant parts derived from soybean variety 5PLSL31, and methods of characterizing soybean variety 5PLSL31. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PLSL31 are further provided.