2116 results about "Germplasm" patented technology

The invention discloses an AsMYB19 gene and application thereof, and relates to the technical field of biology, the nucleotide sequence of the AsMYB19 gene is shown as SEQ ID NO.1, the AsMYB19 gene can regulate and control the tiller number and the yield of oat, gene resources and strategies are provided for oat breeding and improvement of oat germplasm resources, the breeding speed is increased, and the breeding efficiency is improved.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention discloses an AsSN1 gene and application, and relates to the technical field of biology. The nucleotide sequence of the AsSN1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the AsSN1 protein is as shown in SEQ ID NO.2. The AsSN1 gene and the protein provided by the invention can regulate and control the spikelet number of the oats, further regulate and control the yield of the oats, provide important support for oat breeding and germplasm resource improvement, and have important significance for meeting the development requirements of animal husbandry.

The invention discloses a gene and a protein for adjusting tobacco polyphenol aroma substances and application of the gene and the protein. The gene is a tobacco NtMYB28 gene, and the CDS sequence of the tobacco NtMYB28 gene is as shown in SEQ ID No. 1. The invention further discloses application of the tobacco NtMYB28 gene in regulating the content of endogenous polyphenol substances in tobacco and regulating and controlling the metabolic pathway of phenylpropane in tobacco. The key gene NtMYB28 capable of simply and effectively adjusting the content of polyphenol substances in tobacco and the metabolic pathway of phenylpropane is found, and the gene can be applied to efficient cultivation of new high-aroma tobacco materials and development and utilization of new high-value functional tobacco germplasm.

The invention discloses a tuna pond culture strategy optimization method and system based on meteorological influence analysis, and the method comprises the steps: collecting regional meteorological data and pond culture monitoring data, and constructing a multi-modal pond ecological feature vector; establishing a weather-water body dynamic coupling model by using a space-time diagram neural network, and generating water body situation response prediction information; a breeding regulation and control strategy is made based on the prediction information, and strategy self-adaptive optimization is achieved through real-time monitoring data; evaluating the anti-disaster capability of the tuna after the meteorological influence is finished, screening high-quality germplasm resources by combining genetic analysis, and generating an anti-disaster breeding scheme. According to the method, full-chain optimization from meteorological early warning to culture regulation and control to genetic breeding is achieved, and the climate toughness and economic benefits of tuna pond culture are comprehensively improved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

Aiming at the problems of low efficiency, strong subjectivity, insufficient modeling ability, complex hyperspectral data noise, weak waxiness spectrum difference and the like of a traditional rice grain waxiness discrimination method, the invention provides a hyperspectral rice grain waxiness discrimination method based on multi-branch collaborative modeling. The method comprises the following steps: S1, acquiring glutinous and non-glutinous rice grain images by using a hyperspectral imaging system to obtain spectral data; s2, preprocessing spectral data through a combined method of SG smoothing, an asymmetric weighted penalty least square method and multivariate scatter correction to reduce noise and interference; s3, constructing a multi-branch modeling architecture which comprises a CNN local feature extraction module, an SRU spectrum sequence modeling module and a HorNet global high-order modeling module, and outputting a discrimination result through a classifier after multi-branch features are subjected to fusion and pooling attention weighting; and S4, evaluating the performance. According to the method, high-precision lossless discrimination of the waxiness is realized, and a technical support is provided for germplasm screening and quality evaluation in rice breeding.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention relates to the technical field of crop genetic breeding, in particular to a plateau rape adaptive germplasm breeding method based on multi-character collaborative screening, which comprises the following steps of: constructing a multi-character evaluation system covering more than 20 indexes of four categories of agriculture, physiology, quality and stress resistance, and determining character weights by applying principal component analysis, grey relational analysis and analytic hierarchy process; and acquiring data by combining unmanned aerial vehicle multispectral imaging, a ground phenotype platform and a molecular marker technology, and substituting the data into the comprehensive evaluation model for germplasm screening. According to the method, the limitation of traditional single-character breeding is broken through, multi-dimensional collaborative evaluation is achieved, and the plateau rape germplasm breeding efficiency and accuracy are remarkably improved.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qSGC.A8-3 remarkably associated with glucosinolate in brassica napus seeds and application of the molecular marker. A locus qSGC.A8-3 remarkably related to the glucosinolate content of the rape seeds and a peak SNP marker Bn-A08-p12638473 of the locus qSGC.A8-3 are located at 10th, 557th and 348th basic groups of a reference genome A08 chromosome of the rape DarmorV4.1, the average additive effect is 9.6 mu mol / g, and 7.5% of phenotypic variance can be explained. When the PARMS marker designed by the SNP is used for detecting rape germplasm resources, the PARMS marker is simple and convenient to operate and clear in typing, and the average thioglycoside content of AA genotype material seeds is higher than that of GG genotype material seeds, so that the PARMS marker has a good selection effect on the thioglycoside of the rape seeds.

The invention relates to the technical field of plant genetic engineering, and discloses a haplotype identification marker of a rice flood-resistant gene ARF9 and application of the haplotype identification marker. The molecular marker is derived from an exon of an ARF9 gene, is located at the 22009082bp and 22010019bp of a No.4 chromosome of rice, and has a basic group of C. The SNP molecular marker is related to the waterflooding resistance of a male parent oryza longistaminate of perennial rice, and the waterflooding resistance of the male parent oryza longistaminate with a locus genotype of CC is obviously higher than that of rice with a locus genotype of non-AA type. Therefore, the invention provides the loop-mediated isothermal amplification primer group for rapidly identifying the ARF9 gene haploid molecular marker, and the loop-mediated isothermal amplification primer group is used for identifying the ARF9 gene haploid molecular marker so as to rapidly screen out waterflooding-resistant rice. The invention provides a group of primers, the flood-resistant rice can be quickly identified by adopting the primers to perform loop isothermal reaction, the detection cost is low, the detection speed is high, the flood-resistant germplasm can be quickly screened in the rice seedling stage by adopting the method provided by the invention, a time-consuming flooding test does not need to be performed in the later stage, and the breeding process is greatly accelerated.

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention discloses a composition, a kit and a method for detecting a mitochondrial gene of sea eel. A primer probe composition targeting the D-LOOP gene of the sea eel mitochondria is designed, and the nucleotide sequences of the primer probe composition correspond to SEQ ID No.1-3. On the basis, the invention further constructs and optimizes a specific real-time fluorescent PCR detection method for the D-LOOP gene of the sea eel mitochondria. According to the method, a part of sequence of the D-LOOP gene of the mitochondria of the sea eel is taken as a target region, a primer and a TaqMan-MGB probe are designed in a targeted manner, and finally a set of rapid, sensitive and high-specificity detection system is established. The detection system can be effectively applied to adulteration and germplasm identification work of the sea eel, and has a very wide industrial application prospect.

The application is suitable for the technical field of germplasm resources, and provides a germplasm resource data management system.The system comprises: an approval and registration sample management system, a resource survey collection management system, a germplasm resource library position management system, a germplasm resource phenotype database and a germplasm resource genotype database, the approval and registration sample management system manages data of crop standard samples; the germplasm resource phenotype database stores phenotype data of crop germplasm resources; the germplasm resource genotype database stores genotype data of crop germplasm resources; the germplasm resource library position management system manages data of crop germplasm resources; the phenotype data of crop germplasm resources, the genotype data of crop germplasm resources and the data of crop germplasm resources are corresponded through library number coding.The application can scientifically manage crop germplasm resource data, and can also realize internet sharing of crop germplasm resource data.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention relates to the field of gene engineering and molecular breeding, and discloses application of a ZmTCP5 gene in regulation and control of heat resistance of corn. According to the invention, three types of knockout mutants of the gene are obtained by using a CRISPR / Cas9 technology, and three overexpression materials are created. At normal temperature (25-28 DEG C), the material has no obvious phenotypic difference with a wild type; however, under high temperature stress (45 DEG C), the ZmTCP5 knockout mutant has enhanced heat resistance, reduced reactive oxygen species (ROS) accumulation, and significantly increased ROS scavenging enzyme activity, chlorophyll content and photochemical efficiency; on the contrary, the ZmTCP5 overexpression strain is more sensitive to heat stress, which indicates that the ZmTCP5 negatively regulates the heat resistance of the corn. The research provides a theoretical basis for analyzing a heat-resistant molecular mechanism of the corn, and also provides an important gene resource for creating a new germplasm of the heat-resistant corn.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmostpheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention provides a local livestock and poultry variety identification method based on an SNP marker, and relates to the field of variety identification, and the method comprises the following steps: S1, genotype data acquisition, S2, genotype data quality control, S3, variety identification standard library establishment, and S4, bloodline content evaluation. According to the method, on the basis of a standardized molecular identification analysis process of genomic SNP marker bloodline content evaluation, genotype information of a core SNP site is analyzed, so that whole analysis process processing from original genotyping data to variety identity judgment is realized; the method can provide standardized, convenient and efficient molecular detection technical support for protection and utilization of genetic resources of local livestock and poultry in China, germplasm innovation and industrial development.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.