2results about How to "Efficient breeding" patented technology

This invention discloses a method for year-round high-efficiency trellis seedling cultivation and propagation of strawberries. The steps are as follows: S1, constructing cultivation trellises; S2, transplanting mother seedlings from February to mid-March; S3, adjusting the cultivation trellis to a "high" position, placing the bagged mother seedlings on the cultivation trellis, and then managing the seedlings to promote strong growth; S4, from early July to early August, cutting off the runners, retaining 3-4 leaves and 1 bud for cutting propagation; S5, after the cutting propagation is completed, adjusting the cultivation trellis to a "low" position, removing the substrate soil and mother seedlings from the original planting bags, inserting them into new planting bags, filling them with substrate soil, and then placing the mother seedlings on the cultivation trellis; S6, managing the mother seedlings to promote strong growth; S7, flowering and fruiting begin in mid-to-late October. This method is simple, easy to operate, and has low facility investment costs. It is not only suitable for rapid propagation of mother seedlings on "high shelves" in spring and summer and meets the needs of soilless cultivation on "low shelves" in autumn and winter, but also greatly improves facility utilization and increases production efficiency.

The application discloses a method for improving the low-temperature stress resistance of rice, a gene and application thereof. Specifically disclosed is a low-temperature stress resistance gene CTB18 of rice, wherein the nucleotide sequence of the gene is shown as SEQ ID NO:1, and the amino acid sequence of the gene is shown as SEQ ID NO:2. The application obtains a low-temperature sensitive mutant of the rice booting stage by performing chemical mutagenesis on the cold-tolerant rice variety Longdao 5 in the booting stage. Under the condition of low-temperature treatment, the anther of the mutant ctb18-1 develops abnormally, the pollen grains are aborted, and the seed setting rate of the plant is reduced. The application separates and clones the key gene CTB18 for regulating the cold tolerance of the rice booting stage from the mutant, provides a new gene resource for cultivating the low-temperature resistant rice variety, has important significance for the field of rice molecular breeding, and provides an efficient breeding mode for creating the transgenic plant variety, germplasm resource and hybridization parent with low-temperature stress resistance.