9results about How to "Efficient breeding" patented technology

The invention discloses a bacterial strain for generating coronatine based on a new mechanism, and an efficient breeding method and fermentation application of a coronatine high-yield bacterial strain, and relates to the technical field of bioengineering. The preservation number of the bacterial strain for generating coronatine by a new mechanism is CGMCC (China General Microbiological Culture Collection Center) NO.33248, and the bacterial strain is named as Pseudomonas syringae XZY; the method comprises the following steps: mutagenizing XZY, and performing efficient directional breeding to obtain a coronatine high-yield strain carrying an alpha-ABR phenotype, the preservation number of the coronatine high-yield strain is CGMCC (China General Microbiological Culture Collection Center) NO.30035, and the coronatine high-yield strain is named as Pseudomonas syringae XZY02. The coronatine fermentation yield of the strain is greatly improved compared with the fermentation yield before mutation, and in addition, the invention further provides a brand new method for obtaining the coronatine high-yield strain through directional breeding based on improvement of threonine downstream metabolic flow.

The invention discloses a breeding method for fir stem tip tissue culture, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) selecting a stem tip on an excellent seed tree as an explant, sterilizing, inoculating to a callus induction culture medium, and culturing and inducing to obtain a callus; then inoculating to a callus proliferation culture medium to carry out callus proliferation; (2) carrying out hot air treatment on the proliferated callus; and (3) sequentially inoculating the callus treated by the hot air to an adventitious bud induction culture medium and a rooting culture medium to form tissue culture seedlings. According to the breeding method for cedarwood stem tip tissue culture, the culture period is remarkably shortened, operation is easy, cost is low, the method is not limited by seasons, and the survival rate is greatly increased.

The invention relates to the technical field of agricultural cultivation, and particularly discloses a spring rape variety breeding method suitable for large-density planting, which comprises the following steps: collecting and establishing a germplasm resource library, performing preliminary screening according to local conditions, evaluating the growth speed, close planting resistance, disease resistance and quality in detail, and screening out excellent germplasm resources; selecting parents according to germplasm resource characters, designing a hybridization combination scheme, and performing artificial hybridization operation to obtain filial generations; filial generation F1-generation microspores are extracted for in-vitro culture, haplobionts are obtained and doubled into a homozygous DH line, and plants meeting the breeding target are screened out; designing a high-density planting test scheme, carrying out a planting test, regularly observing and recording plant characters, and screening out a spring rape variety which is excellent in performance and meets a breeding target; the breeding steps of the system comprise germplasm resource screening, hybrid combination construction, microspore culture, DH line breeding and high-density planting test and screening.

This invention discloses a method for year-round high-efficiency trellis seedling cultivation and propagation of strawberries. The steps are as follows: S1, constructing cultivation trellises; S2, transplanting mother seedlings from February to mid-March; S3, adjusting the cultivation trellis to a "high" position, placing the bagged mother seedlings on the cultivation trellis, and then managing the seedlings to promote strong growth; S4, from early July to early August, cutting off the runners, retaining 3-4 leaves and 1 bud for cutting propagation; S5, after the cutting propagation is completed, adjusting the cultivation trellis to a "low" position, removing the substrate soil and mother seedlings from the original planting bags, inserting them into new planting bags, filling them with substrate soil, and then placing the mother seedlings on the cultivation trellis; S6, managing the mother seedlings to promote strong growth; S7, flowering and fruiting begin in mid-to-late October. This method is simple, easy to operate, and has low facility investment costs. It is not only suitable for rapid propagation of mother seedlings on "high shelves" in spring and summer and meets the needs of soilless cultivation on "low shelves" in autumn and winter, but also greatly improves facility utilization and increases production efficiency.

The invention relates to the technical field of data processing, and particularly provides a system and method for generating a newborn file of an animal baby, a storage medium and electronic equipment, and the system can comprise a marking module which is used for marking the baby meeting the requirement through an identity sensor of the baby to obtain a marked baby; the weighing module is used for obtaining the weight of the marked baby in multiple stages, and the multiple stages comprise the birth stage and the lactation ending stage; the behavior detecting and sensing module is used for acquiring behavior data of the marked baby in the lactation stage; and the cloud service platform is used for generating a newborn archive at least based on the weight of the baby, the behavior data, the identity information of the baby corresponding to the marked baby, the parent information and the parent information. According to some embodiments of the invention, accurate management of the baby birth data can be realized.

The invention relates to the technical field of biological prevention and control of agricultural and forestry pests, in particular to an artificial breeding method of short-stalk long-tail encardia. The method comprises the following steps: by taking oak eggs as hosts for bee inoculation and a fructose aqueous solution as a sugar source, inoculating 3-day-old female bees which have reproductive memory and are copulated, and carrying out bee inoculation treatment twice; and breeding the tussah eggs on which the short-stalk long-tail bees parasitize, which are collected by the two-time bee inoculation treatment, so as to obtain the short-stalk long-tail bees. On the basis of reproductive memory and in combination with the biological characteristics of the short-peduncle long-tail bees, influence factors such as the most suitable mother bee day age, bee grafting proportion and time of the short-peduncle long-tail bees are determined, the phenomenon of low parasitic rate or over-parasitism caused by improper parasitic proportion or time is avoided, and the survival rate of the short-peduncle long-tail bees is improved. And therefore, the parasitism rate and eclosion rate of the short-stalk long-tailed encardia are improved.

The invention discloses a cutting propagation method of Robinia pseudoacacia, and belongs to the technical field of seedling propagation. The cutting propagation method comprises the following steps: (1) substrate and cutting bed processing; (2) cutting selection and processing: using Robinia pseudoacacia pinnate leaves as cuttings, and inserting them as soon as they are picked; (3) cutting; (4) post-cutting management. The invention uses Robinia pseudoacacia pinnate leaves as cutting materials, breaking through the selection range of Robinia pseudoacacia cutting propagation materials in the past, ensuring the stable retention of excellent traits of the propagation offspring materials, especially when the mother plant has branch mutations and the material is less, it can achieve rapid and efficient propagation of Robinia pseudoacacia.

The invention relates to the technical field of plant culture, and particularly discloses an open type tissue culture method and application of dionaea muscipula. Comprising the following steps: (1) selecting leaves of dionaea muscipula, cleaning and disinfecting; (2) preparing a liquid culture medium A; (3) adding a liquid culture medium A and the disinfected dionaea muscipula leaves into a container, sealing, and culturing under red light to obtain dionaea muscipula leaves with dense adventitious buds; (4) preparing a liquid culture medium B; (5) replacing the culture medium with a liquid culture medium B in the container, adding the dionaea muscipula leaves with dense adventitious buds, sealing, and culturing under the common illumination of red light and blue light to obtain the dionaea muscipula leaves with roots; and (6) taking out the leaves of the dionaea muscipula with roots, cleaning, planting in a matrix, and performing seedling hardening culture under natural light to complete open tissue culture of the dionaea muscipula. The open type tissue culture method of the dionaea muscipula provided by the invention achieves the effects of shorter tissue culture time and more efficient breeding.

The application discloses a method for improving the low-temperature stress resistance of rice, a gene and application thereof. Specifically disclosed is a low-temperature stress resistance gene CTB18 of rice, wherein the nucleotide sequence of the gene is shown as SEQ ID NO:1, and the amino acid sequence of the gene is shown as SEQ ID NO:2. The application obtains a low-temperature sensitive mutant of the rice booting stage by performing chemical mutagenesis on the cold-tolerant rice variety Longdao 5 in the booting stage. Under the condition of low-temperature treatment, the anther of the mutant ctb18-1 develops abnormally, the pollen grains are aborted, and the seed setting rate of the plant is reduced. The application separates and clones the key gene CTB18 for regulating the cold tolerance of the rice booting stage from the mutant, provides a new gene resource for cultivating the low-temperature resistant rice variety, has important significance for the field of rice molecular breeding, and provides an efficient breeding mode for creating the transgenic plant variety, germplasm resource and hybridization parent with low-temperature stress resistance.