Cultivating method for arundina chinensis and application thereof

A cultivation method, the technology of bamboo leaf orchid, applied in the field of plant cultivation, to achieve the effect of improving efficiency, saving production cost and cycle, and promoting the accumulation of plant chlorophyll

Active Publication Date: 2018-02-02
中山市西江花木研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research focuses on species with relatively mature large-scale cultivation techniques, and there is no report on the regulation of light quality in the planting process of Bambooleaf orchid.

Method used

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  • Cultivating method for arundina chinensis and application thereof
  • Cultivating method for arundina chinensis and application thereof
  • Cultivating method for arundina chinensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 sterile seeding medium optimization

[0027] After picking the bamboo leaf orchid capsules that have grown for 28-30 days after pollination, disinfect them with 70% alcohol for 60 seconds, wash them with sterile water, and then wash them with 0.1% HgCl 2 Sterilize the solution for 15 minutes, then rinse with sterile water for 3 to 5 times, and finally blot the surface water with sterile filter paper, cut the sterilized capsules with a scalpel, take out the seeds and spread them to the sows that have been sterilized by high temperature and high pressure medium.

[0028] The culture medium is Huabao No. 1 medium + agar 7g / L + sucrose 30g / L as the control group, add 6-benzylpurine (6-BA) 0.5~1.5mg / L, naphthaleneacetic acid (NAA) 0.1~0.2mg / L L, activated carbon 0.5 ~ 1g / L, banana puree 10 ~ 30g / L is the optimal group, the specific ratio is shown in Table 1. pH value = 5.6-5.8.

[0029] Table 1 seeding medium formula

[0030] Medium formula

[0031...

Embodiment 2

[0035] Embodiment 2 subculture growth medium component optimization

[0036] The new shoots in the seeding medium are inoculated into the secondary growth medium for rapid growth culture. 10 buds were inoculated per bottle, 10 bottles per treatment. The culture medium is Huabao No. 1 medium + agar 7g / L + sucrose 30g / L as the control group, in which 0.5-3mg / L of 6-benzylpurine (6-BA) and 0.2-1.0mg / L of naphthaleneacetic acid (NAA) are added. L, activated carbon 0.5 ~ 1g / L, banana puree 10 ~ 30g / L is the optimal group, the specific ratio is shown in Table 3. pH value = 5.6-5.8.

[0037] Table 3 Subculture Growth Medium Formula

[0038] Medium formula

[0039] The culture conditions are photoperiod 16h / d, light intensity 45μmo1 m -2 ·s -1 , the ambient temperature is 24±1°C, and the relative humidity is 50-60%. After the test-tube seedlings were continuously cultured for 60 days, the average plant height of the inoculated buds, the average number of leaves and fre...

Embodiment 3

[0043] Embodiment 3, the use effect of culture medium with LED

[0044] 1. Subculture growth medium

[0045] Huabao No. 1 medium+6-BA1.0mg / L+NAA0.5mg / L+activated carbon 0.5g / L+sucrose 30g / L+banana puree 30g / L+agar 7g / L solid medium (pH=5.6~5.8).

[0046] 2. Inoculation method

[0047] 60 days after sowing, the seedlings of Bambooleaf orchid were inoculated into the subculture growth medium for proliferation and culture. 10 buds were inoculated per bottle, 10 bottles per treatment.

[0048] 3. Culture conditions

[0049] The photoperiod is 16h / d, and the light intensity is 45μmo1·m -2 ·s -1 , the ambient temperature is 24±1℃. Nine treatments were set up in the experiment, see Table 5 for details. Taking white fluorescent lamp (WFL) as a control, the LED light source treatments are warm white light (WW), cool white light (CW), far red light (DR), red light (R) , blue light (B), red and blue ratio light (R: B = 3: 1, R: B = 2: 2, R: B = 1: 3 and below are represented by 3R...

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Abstract

The invention discloses a cultivating method for arundina chinensis and an application thereof. The cultivating method comprises the following steps: picking the arundina chinensis capsule which growsfor 28-30 days after the fertilization, and performing the aseptic insemination; and transplanting obtained protocorm in a subculture growth medium, inducing the protocorm to grow and sprout rapidly,using an LED as a tissue culture light source, and performing the red-light and blue-light combined cultivation according to the proportion of 2-4:6-8; transplanting aseptic seedlings of which the height is more than 7 cm in a greenhouse, and performing the cultivation management. The method is capable of determining the optimal culture conditions in the subculture process after the seed germination of the arundina chinensis, wherein the optimal culture conditions comprise LED light quality and light intensity and treatment time, effectively promoting the accumulation of plant chlorophy, thethickening of stems and the extension of root, and saving the production cost and period. The seedlings can be directly transplanted after the subculture without the strong seedling and rooting process. Especially, the LED promote the flavonoid and phenolic substance accumulation of active ingredients in the arundina chinensis, the oxidation resistance of plants is remarkably improved, and the method has the important significance to the medicinal plant cultivation.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for cultivating bamboo leaf orchid and its application. Background technique [0002] Arundina graminifolia (Arundina graminifolia) is a ground-grown species of Orchidaceae. It has the functions of regulating qi and blood, clearing fire and detoxifying, and is one of the main raw materials of Dai medicine Dai Baijie (Baijie Capsule). A large number of studies have found that the extract of bamboo leaf orchid has high antioxidant, anti-tumor and anti-viral activities and anti-lipid peroxidation, so it can be used to treat diseases such as food, poisonous bacteria, and drug poisoning. Among them, polyphenols, flavonoids , saponins and other content showed a significant dose effect. In addition, the flowers of bamboo leaf orchid are similar to cattleya, with large and beautiful flowers, continuous flowering throughout the year and strong environmental adaptabilit...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨凤玺朱根发郭阿瑾陆楚桥金建鹏魏永路高洁
Owner 中山市西江花木研究有限公司
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