The invention relates to an enzymatical extraction process of oil in tea seed oil kernel, which comprises the following steps: firstly, utilizing dried tea seed oil kernel to be raw material, immersing in water in high temperature after disintegrating, stirring in uniform speed under 70-100 DEG C, secondly, enzyme hydrolyzing, adding acid protease which is 0.02-1% dry weight of disintegration samples or mixed enzyme of acid protease/cellulose in 45-55 DEG C to stir in uniform speed, and enzymatic hydrolysis for 2-4 hours, thirdly, separation emulsion breaking processing, directly separating out primary free boiled oil after enzymolysis liquid is centrifuged, getting secondary free boiled oil through centrifuging after missible oil demulsifies, combining the primary free boiled oil and the secondary free boiled oil, and getting the total boiled oil. The invention has the advantages that first, the invention reduces the problem of emulsification, foaming and the like which are faced when a grinding method is utilized to destroy cell structures, second, the invention prevents various enzymes in cells from degrading oil, which has high oil extraction rate to a further disintegrated function to tea seed oil kernel cell, third, the invention increases the treatment to missible oil, which increases free boiled oil yield through demulsifying, centrifuging, stewing to layer and the other modes.