386results about How to "Short breeding cycle" patented technology

The invention discloses a method for growing watermelons. The method includes the steps of processing seeds and accelerating germination, sowing and raising seedlings, applying base fertilizer on the whole land, transplanting the seedlings, performing field management, harvesting and the like. The method for growing watermelons is easy to operate, convenient to manage, good in growth vigor of watermelons, high in disease resistance, uniform in result nature and high in yield, the yield per mu can reach 4000 kg to 5000 kg, the watermelons are large, good in quality, high in sugar content, bright in flesh color, delicate in flesh and high in water content and commodity value, and therefore the method has great significance for increasing income of melon farmers.

The invention relates to a plant seedling raising technique, in particular to a camellia oleifera container seedling raising technique which aims to solve the problems of low seedling survival rate and low seedling raising efficiency of a camellia oleifera seedling raising technique in the prior art. The camellia oleifera container seedling raising technique includes the steps of (1) collecting seeds, and placing the collected seeds in a refrigeratory for cold storage and reserve at the temperature of 0-5 DEG C; (2) pre-treating the seeds, and soaking the seeds prior to sand storage for accelerating germination; (3) sowing, sowing the camellia oleifera seeds subjected to germination accelerating in a container containing seedling raising substrates in a spot manner, and then placing the container on a seedbed; (4) grating rootstocks of seedlings; (5) performing grafted seedling management including water management, rootstock removal and weeding management, light and temperature management, nutrient management and the like; (6) performing biennial seedling production management; and (7) performing outplanting management. The technique has the advantages of high seedling survival rate, high breeding efficiency and good reliability; and seedling survival rate of the seedling raising technique is 10-20% higher than that of inarching seedling raising and leaf cutting seedling raising techniques.

The invention relates to a water culture method in raising seedlings of vegetable cavernosum, belonging to the technical field of vegetable seedling. The invention is characterized in that, the method has the matching between the water culture facility in raising seedlings of vegetable cavernosum and a nutrient solution specialized for seedlings; year-round production of water cultured vegetable seedlings is realized. The water culture method in raising seedlings of vegetable cavernosum has the advantages that, the growth process of the invention does not need to adopt convention nursery substrate; the drawbacks of conventional plug seedlings that the conventional plug seedlings are easy to be influenced by the nursery substrate and the conventional plug seedlings are not suitable to be used as water cultured vegetable are overcome; the nutrient required by the growth of the cultivated seedlings is provided by the special nutrient solution; the invention has the advantages of neat seedlings, no recovery period, short seedling period, high strong seedlings rate and other excellences.

The invention provides a breeding method capable of changing the glume color of rice varieties with yellow glume to brownness. The breeding method comprises the following steps: selecting a target fragment in an extron region of a glume color determining gene OsCAD2, constructing a plant CRISPR/Cas9 targeting recombinant vector, guiding the recombination vector into a rice cell, regenerating the rice cell into seedlings, and sequencing genome target fragments of regenerated strains so as to obtain the strains, two allelic genes OsCAD2 of which simultaneously suffer function deletion mutation. Through phenotype identification, the change of the glume color is realized. Experiments prove that the breeding method can quickly change the glume color of the rice varieties.

A tissue culturing method for quercus virginiana belongs to plant tissue culturing methods and is characterized by comprising the following steps: 1 selecting of explants; 2 disinfection of the explants; 3 primary culture; 4 multiplication culturing of adventitious buds; 5 culturing of strong seedlings; 6 culturing of strike roots; and 7 seedling acclimatization and transplanting. The tissue culturing method for the quercus virginiana can keep stress resistance and enjoyment of a female parent and can effectively increase propagation coefficient of the quercus virginiana. Compared with seminal propagation, tissue culturing reduces dependency on imported seeds, can effectively reduce nursery stock cost, and saves expenditures. Compared with cutting propagation, the tissue culturing is short in seedling culturing period, can obtain the nursery stock with strike roots within 4 months, and saves cost.

The invention discloses a pure line breeding method for increasing the local fowl breed egg yield. The method includes the following steps that (1) the number of eggs laid by individual hens ranging from 21-week-old to 32-week-old, the number of eggs laid by individual hens in the egg laying peak week and the number of weeks of the egg laying peak weeks of all the individual hens are detected and determined; (2) parameter indexes of reserved eggs for breeding are stipulated; the number of the eggs laid by the selected individual breeding hens ranging from 21-week-old to 32-week-old is larger than or equal to 42, the number of the eggs laid by all the individual hens in the egg laying peak week is larger than or equal to 5, and the number of the weeks of the egg laying peak weeks of all the individual hens is larger than or equal to 1, and the number is increased generation by generation; (3) breeding cocks and breeding hens are reserved and mated to form new families, the cocks and the hens which are of the homologous series but not near of kin are selected to form new families, the proportion of the cocks and the hens is 1:10-16, and over forty families form a pure line to breed the next generation of breeding chicken; step (2) and step (3) are carried out repeatedly till over three generations are bred. By means of the method, the egg yield of local chicken can be increased effectively and quickly, the variety breeding effect is remarkable for the local chicken with the low egg yield, the egg yield of breeding chicken is increased over 40% after four generations of breeding, and the generation breeding cycle is advanced by over 10 weeks.

The invention provides a lysinecorn SSR (simple sequence repeat) molecular marker auxiliary selecting and breeding method. By combining a backcross transforming method with a molecular marking method, the method successfully constructs the lysinecorn isogenic gene systems QZ58, Q478 and QC72. After the SSR molecular marker auxiliary selecting and breeding method is used the mono-clones which contain the O2 gene can be detected by SSR molecular marker in the O2 gene after one generation is backcrossed, and the second generation can be backcrossed without selfing, so that the breeding is short in period, low in cost, and high in efficiency. Aiming at different breeding target requirements, the method is used for performing the gene type detection to the backcross offspring seedling leaves; and the method is not influenced by the environment conditions and combines with south propagating and generation adding, so that the target genes are transferred into a good material within a shorter time.

The invention discloses a tomato fruit ripening gene SINAC3 and application thereof. The tomato fruit ripening gene SINAC3 has a nucleotide sequence shown as SEQIDNO.1 or protein of an amino acid sequence coded as SEQIDNO.2, a typical NAC homology domain and coded NAC protein. As proved by the relevant technology of functional genomics, the tomato fruit ripening gene SINAC3 plays an important role in tomato fruit ripening. The tomato fruit ripening gene SINAC3 is structured onto a plant expression vector and transferred into a tomato for interference expression, the transgenic tomato can show the capacity of delaying fruit ripening, and the tomato fruit ripening gene SINAC3 can be directly used for controlling the ripening progress of fruits in production.

The invention provides a device for combining microalgae cultivation and sewage treatment. The device comprises a first container, a second container and a third container which are made of a light-transmitting material, wherein a water outlet of the first container is connected with a water inlet of the second container; the second container is sleeved in the third container; an aerating device is arranged at the bottom of the first container; a carbon dioxide spraying device, a microalgae liquid spraying device and an oxygen collecting device are arranged at the bottom of the second container; the bottom of the second container is communicated with the third container; the bottom of the third container is conical, and a spoil disposal pipe is arranged at the bottom of the third container; and a microalgae harvesting pipe is arranged on the side wall of the third container. The device can provide ideal intergrowth environment for algae and bacteria, cultivate the microalgae for producing biodiesel and perform sewage treatment to remove organic matters and phospho-nitrogen from water effectively and reduce the chance of bursting blue-green algae in a large area in water bodies of China. The device has the advantages of short cultivating period and high yield of the microalgae, can realize the industrial production of the microalgae, and is favorable for environmental protection.

The invention provides a breeding method for high-oleic acid high-yield peanut, which includes steps: (1) coordinate parent combination: selecting high-yield germplasm with good integrated character and germplasm with high oleic acid content SPI098 to combine patent; (2) high oleic acid pressure screening: determining oleic acid of each seed in excellent single plant in early separation generation, and selecting excellent peanut seed with high oleic acid content; (3) high-yield pressure screening: planting the screened peanut seed with high oleic acid content in next generation, separating generation in anaphase, and selecting excellent high-yield single plant; and (4) high-oleic acid high-yield peanut variety or strain obtaining: obtaining novel high-oleic acid high-yield peanut variety or strain through multi-generation high-oleic acid high-yield pressure screening. The breeding method provided by the present invention directly screens according to character of oleic acid content in peanut seed, and has advantages of short breeding period, small blindness, high efficiency, and good economic benefit and social benefit.

The invention relates to a micrografting method for a tetraploid acacia. The method includes the steps of selecting a seedling of a diploid acacia seed as a rootstock, selecting a tissue culture seedling of the tetraploid acacia as a scion, subjecting the scion and the rootstock to cambium alignment by using a cleft grafting method, twining the interface area with silver paper slips, fixing the silver paper twining area with a grafting clamp, planting the grafted seedling into a potted tray containing matrix, then processing a routine management for 20 - 30 days, and acquiring the plant regeneration. By means of the micrografting method, an integral plant is obtained after cultivating the grafted seedling for 20 - 30 days, and accordingly the high efficiency of rapid planting is achieved. The plant regeneration cultivated by the micrografting method has the advantages of stable genetic character, healthy plant, strong growth, short seedling period, fast plant growing and high survival rate ( with a survival rate over 90% in a field planting production land).

The invention discloses a breeding method of an induction line for efficiently identifying a corn haploid young embryo. The method comprises the following steps of hybridizing a corn haploid inductionline with a corn inbred line S23 to obtain a hybrid F<1> generation; backcrossing the hybrid F<1> generation and the corn haploid induction line to obtain a backcross posterity BC<1>F<1>; selecting asingle plant of the corn from the backcross posterity BC<1>F<1> to continuously self-cross to obtain the induction line for efficiently identifying the corn haploid young embryo, wherein the single plant of the corn is high in color developing degree of the young embryo after being hybridized with corn hybrids, high in haploid induction rate and high single-ear haploid number after being hybridized with the corn hybrids and is homozygous for both Zmpla1 gene and Zmdmp gene; and the haploid inductivity, the single-ear haploid number, the plant height, the ear position, the tassel branch number, the single-ear fructification number and the haploid young embryo identification accuracy of the induction line for efficiently identifying the corn haploid young embryo are all higher than those ofthe corn haploid induction line. The breeding method of the induction line for efficiently identifying the corn haploid young embryo has important application value.

The invention provides an open-air seedling raising method through seedling trays, which comprises the following steps of: arrangement of a seedling bed, namely selecting the original seedling raising bed, performing soil preparation by a water raising method, feeding water to the seedling bed, making furrows, dredging a furrow trench on the side of each furrow, merging slurry into the furrow trenches, and scattering a compound fertilizer in the furrow trenches; the placement of the seedling trays and slurry filling, namely placing the seedling trays on the surfaces of furrows and filling the slurry in the furrow trenches into the seedling trays; germination hastening and seeding, namely germinating grain seeds, scattering the germinated grain seeds on the surface of the slurry in the seedling trays, and smearing the slurry; and the management of seed beds, namely after seeding, airing the bed to raise seedlings, sprinkling herbicide when the seedlings are raised to reach the two-leaf and one-core period, watering and applying urea to the seedling trays in the second day after sprinkling the herbicide, draining water of the seedling trays after two days, keeping the semiarid state of the seedling trays, and removing the seedling trays after the seedlings are raised to reach the four-leaf and one-core period. The method has the advantages of short seedling raising period, simple seedling raising process, low cost, time saving, labor saving, no plant diseases and insect pests of the raised seedlings and low incidence of diseases of the seedlings after seedling transplantation.

The invention provides a Dioscoreae Oppositae 'Qi' tissue culture seedling raising method, which comprises the steps of explant selection and disinfection, callus tissue induction, adventitious bud differentiation, rooting culture, seedling training, transplanting and the like. By taking the nodular stem segments of the potted Dioscoreae Oppositae 'Qi' as explants, after the explants are disinfected by alcohol with volume concentration of 70 percent and sodium hypochlorite with weigh percentage of 2 percent, the callus tissue induction, the adventitious bud differentiation and the rooting culture are conducted on MS+NAA+BA culture mediums, and rooted seedlings are transplanted in mixed mediums for culture, wherein the mixed mediums comprise vegetable garden soil, mountain sand and peat, with the mass ratio of 4:1:1. Compared with traditional Dioscoreae Oppositae 'Qi' seedling raising methods such as a direct root tuber planting method, a cutting breeding method and the like, the Dioscoreae Oppositae 'Qi' tissue culture seedling raising method has the advantages that the genetic stability of the seedlings is good, the breeding cycle is short, the breeding coefficient is high, the operation is simple and the factory production of the seedlings can be realized. By adopting the method, not only can the problems that the quantity of the used seminal roots for the production of the Dioscoreae Oppositae 'Qi' is large, the cost is high and the characters of strain is degenerated be solved, but also technical supports can be provided for the high quality and high yield and the industrialization of the Dioscoreae Oppositae 'Qi' through large-scale seedling breeding.

The invention discloses a thin-shell pecan tissue culture bud micro grafting method which comprises the following steps of: 1. inducing and multiplying a scion tissue culture bud, wherein five working procedures of collecting and disinfecting an explant, preparing an adventitious bud inducing culture medium, inducing an adventitious bud, preparing an adventitious bud multiplying culture medium and performing adventitious bud multiplying cultivation; 2. cultivating a thin-shell pecan stock; 3. grafting the tissue culture bud; and 4. managing grafting and a healing stage. According to the method, the thin-shell pecan has the advantages of retaining the maternal characteristics along with early bearing, being high in inversion resistance, high in multiplying speed and the like.

The invention discloses a method for breeding high-yield high-oleic-acid high-oil peanuts, which comprises the following steps: crossing a high-yield high-oleic-acid peanut variety and a high-yield high-oil peanut variety; backcrossing the hybrid generations with the parent generations respectively; continuing to backcross high-oleic-acid single plants from AC1F1 generation and high-oil single plants from BC1F1 respectively to obtain AC2F1 and BC2F1 respectively; performing selfing of the AC2F1 and selfing of BC2F1 respectively; after selfing, performing crossing to obtain secondary crossing generation; performing continuous multi-generation selfing by starting from the secondary crossing generation, selecting high-yield high-oleic-acid high-oil single plants for selfing propagation from each generation after the F2 generation and while selecting high-yield single plants, performing high-oleic-acid and high-oil nondestructive selection by using nondestructive selection technology, andperforming the selfing of the selected high-yield high-oleic-acid high-oil single plants; and performing comparative selection of lines from F6 generation and obtaining new high-yield high-oleic-acidhigh-oil peanut variety.

The present invention relates to the technical field of plant seedling breeding, in particular to a one-step cultivation method for Camellia oleifera bud-stock light matrix grafting container seedlings. The method comprises seedbed preparation, seedling transplantation and incubation management after seedling transplantation. The seedbed preparation comprises the steps of shadow sprinkler system construction, seedbed establishment, overall disinfection, nutrition frame placement and matrix disinfection, the seedling transplantation comprises the steps of sludge preparation, matrix treatment, seedling transplantation, spraying disinfection, rodenticide placement and film covering, and the incubation management after seedling transplantation comprises the steps of complementation and moisture retention, spraying cooling, film-peeling protection, and film and shadow net removal. The present invention aims to provide a one-step cultivation method for Camellia oleifera bud-stock light matrix grafting container seedlings, the conventional method is changed, and a centralized and refined management process is adopted, so that the graft survival rate and seedling growth speed are improved, and then the afforestation efficiency is improved, and the economic effect is increased.

The invention discloses a damask rose twig cutting seedling-raising method which includes the steps: S1 performing laying and disinfecting of seedling beds; S2 performing preparation and disinfection of culture media and layout of plugs; S3 cutting semi-lignified twigs without diseases and insect pests on stock plants, performing water spraying and moisturizing on the cut semi-lignified twigs, cutting cuttings at shade places, and performing disinfection on the cut cuttings; S4 plunging deep holes in the culture media, treating the disinfected cuttings by the aid of rooting agents, performing cutting on the treated cuttings, lightly pushing sealing openings with fingers after cutting, and enabling the culture media and the cuttings to be closely connected; S5 performing reasonable atomizing and light supplement management after cutting. 2-3 bud joints are reserved on each cutting, the length of each cutting is about 8cm-10cm, 4 leaflets of the upper portion of each cutting are reserved, the position 5 millimeters away from the uppermost bud joint of each cutting is horizontally cut, the position 3-5 millimeters away from lowermost bud joint of each cutting is cut into a 45-degree inclined plane, and the cutting depth does not exceed 3cm. The method is rapid in rooting, high in transplanting survival rate, simple and easy to operate, and a seedling-raising period can be greatly shortened.

The invention discloses a high-efficiency ecological breeding method for South American white shrimps. The high-efficiency ecological breeding method includes the steps such as breeding greenhouse building, offspring seed selection, stocking preparation, breeding management, water quality management. The technology is advanced and applicable; operability is strong; and breeding risk of South American white shrimps is reduced. Disease incidence is reduced. Dissolved oxygen of varied water layers in greenhouses is improved; condition of bottom layer water is improved; improvement in appetites of the South American white shrimps is benefited; and digestibility and utilization rate of feeds are increased.