3results about How to "Short breeding cycle" patented technology

This invention belongs to the field of crop cultivation technology and discloses a method for rapid generation of lettuce. The method involves: hydroponically cultivating lettuce seeds into seedlings, which are then transplanted after reaching the stage of two leaves and one bud; the seedlings are then cultured in a hydroponic tank with a total nitrogen nutrient solution at a nitrogen concentration of 9.0 mmol / L, a temperature of 25℃, and a light intensity maintained at 200 μmol·m² for the first 5 days. ‑2 ·s ‑1 The photoperiod is 16:8, the light quality is red:blue:far-red = 6:1:1, and the light intensity is increased to 320 μmol·m⁻¹ starting from day 6. ‑2 ·s ‑1 The photoperiod was 16:8, the light quality was red:blue:far-red = 6:1:1, and the plants were cultured for 15 days. The cultured lettuce plants were then placed in a low-nitrogen nutrient solution with a nitrogen concentration of 1.5 mmol / L at 25℃ and a light intensity of 320 μmol·m⁻¹. ‑2 ·s ‑1 The photoperiod was 20:4, and the light quality was red:blue:far-red = 6:1:1. The plants were cultured until flowering. After flowering, the nitrogen concentration of the low-nitrogen nutrient solution was adjusted to the normal nitrogen concentration of 9.0 mmol / L until the seeds matured. This invention promotes rapid flowering and seed setting in lettuce, shortens the breeding cycle, and accelerates the lettuce breeding process.

This invention discloses a grafting propagation method for the summer wax plum 'Hongyun'. The method involves applying nitrogen fertilizer, boron fertilizer, and humic acid in the autumn of the previous year to build up energy reserves. In early March of the same year, a compound hormone of GA3+6-BA+NAA is sprayed three times. Combined with black plastic film wrapping to increase temperature and block light, black plastic film mulching to retain moisture, and ridge planting with frequent water management, the cambium layer of the wax plum rootstock and summer wax plum scion branches becomes active and ready for peeling in early spring. At this time, large-block budding is performed, resulting in high scion utilization. The buds sprout in early April, and the scions can be ready for transplanting in November of the same year. This method achieves a grafting survival rate of over 82%, which is more than 16 percentage points higher than traditional summer budding. The transplanting rate in the same year reaches 88%, the seedling cycle is shortened by one year, and the scion utilization rate is increased by 110%. This provides reliable technical support for the large-scale propagation of the rare new variety 'Hongyun'.

The present invention discloses a method for cultivating double haploid of fragrant green vegetable by inducing parthenogenesis through distant hybridization. Using non-heading Chinese cabbage 'Fragrant Green Vegetable' as the female parent and broad bean 'Japanese Big White Skin' as the male parent, artificial pollination hybridization is carried out, and brassinolide solution is sprayed to induce parthenogenesis, so as to cultivate the double haploid of fragrant green vegetable. As long as artificial cross-pollination is carried out and combined with the application of brassinolide solution, the double haploid of fragrant green vegetable can be obtained, which can accelerate the rate of cultivating high-quality germplasm resources of fragrant green vegetable.