The invention discloses a plastic film mulching cultivation method suitable for autumn-planted peanuts in subtropical regions. The method is as follows: carrying out soil preparation as soon as possible after harvesting previous crops, combining the oil preparation with fertilization together, and adopting the one-time fertilization way; firstly mulching a film for covering the soil before seeding, and utilizing the high temperature in late summer or early autumn in the subtropical regions for killing partial pathogens, weeds and pests in the soil; adopting the technology of firstly punching and then seeding, and seeding peanut seeds in holes without covering the seeds in the plastic film for preventing the high temperature from baking the seeds; adopting the technology of sequentially covering the soil, the seeds and the film for preventing hot airflow in the film from escaping and burning seedlings; and simultaneously effectively reducing water content in the soil after heavy rain and being conductive to the germination of the peanut seeds. The method is simple, practical, easy to master and convenient to popularize, and can solve the problem that the plastic film mulching technology can not be applied in the autumn-planted peanuts in the subtropical regions, significantly improve the output of the autumn-planted peanuts in the subtropical regions and be particularly applicable to the cultivation of the autumn-planted peanuts in dry land and rain-fed fields in rain-fed areas of the subtropical regions.