Manufacturing method for microbial manure for subtropical forest sprouts
A technology for microbial organic fertilizer and forest tree seedlings, applied in the field of microbial fermentation engineering, can solve problems such as no research reports, and achieve the effects of promoting plant growth, improving fertilizer efficiency and balancing species.
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[0023] 1. Microbial strains:
[0024] Bacillus polymyxa ATCC842; Bacillus megatherium ATCC14815; Bacillus subtilius ATCC6051; Bacillus licheniformis ATCC14580; Bacillus mucilaginosus 1.1737 Rhodospseudomonas sphaerioide).
[0025] 1.1 The methods for large-scale expansion of Bacillus polymyxa, Bacillus megaterium, Bacillus subtilis, Bacillus glaci, and Bacillus licheniformis are as follows:
[0026] Bacillus polymyxa, Bacillus megaterium, Bacillus subtilis, Bacillus coli, Bacillus licheniformis liquid seed medium (g / L): peptone 10.0, yeast extract 10.0, glucose 60.0, MgSO 4 ·7H 2 O 0.5, the rest of the water, pH 7.2 ~ 7.4, 121 ℃ sterilization for 20 minutes. According to the conventional method of expanding culture, each kind of bacteria is cultivated separately.
[0027] 1.2 The cultivation method of Rhodopseudomonas sphaeroides is as follows:
[0028] 1) Expansion of training at one time
[0029] Medium:
[0030] Yeast extract 5g, glucose 15g
[0031] 15 grams of ammonium chlorid...
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