1476results about How to "Strong stress resistance" patented technology

The invention discloses a rice slow-release fertilizer and a preparation method thereof. The rice slow-release fertilizer comprises the following raw material components by weight: 25-40 parts of compound bacteria, 500-600 parts of an organic material, 800-1200 parts of an inorganic material, 100-150 parts of a fertilizer core, 20-30 parts of trace elements, 120-150 parts of additives, 70-80 partsof cellulose and 2500-3500 parts of a solvent.

The invention discloses an enterococcus faecium and application thereof. An enterococcus faecium strain provided by the invention is enterococcus faecium suppressant 80, and the collection number is CGMCC No.5058. As proved by experiments, the enterococcus faecium suppressant 80 is obtained by separating, identifying and screening; and the enterococcus faecium suppressant 80 has high resistance, high bacterial resistance and probiotic characteristics, and can be taken as an additive for preparing animal feeds; and the animals include but is not limited to various animals such as pigs, cows, sheep, chicken and the like. The feeds have similar functions to antibiotic feeds, but do not have the side effects of the antibiotic feeds.

The invention provides a compound bio-fertilizer containing ardealite and application thereof. Compound bio-fertilizer is obtained through following steps: adjusting pH value of the ardealite to 4-5 via lime water, drying and crushing to 200-400 sections; drying animal manure, cutting crop straws into sections and then drying; mixing and fermenting the ardelite, the animal manure and the crop straws; controlling cultivation temperature to be at 28-32 DEG C after medium sterilization, sequentially inoculating various beneficial microorganisms, respectively cultivating the same, mixing mixed bacteria with prepared fermenting mixture by weight ratio of 1:300-1000 after completing cultivation, adding water to adjust water content to 35-45%, stirring the water , stacking the mixture for 3-5 days for co-fermentation, further adding water to adjust water content to 20-30%, and then crushing to obtain the compound bio-fertilizer. The compound bio-fertilizer is mainly applied to field crops and applied to improving saline-alkali soil, acid soil and coastal soil structures.

The invention belongs to the field of biological techniques and relates to a bacillus subtilis strain and a feed additive and a fermenting agent thereof. The bacillus subtilis has high stress resistance and probiotic effect, so the bacillus subtilis can tolerate artificial gastric juice with a pH value of 2.0 and a concentration of 1 percent, artificial cholate at a concentration of 0.3 percent and a granulating temperature of 80 DEG C; and the bacillus subtilis has a strong inhibiting effect on Escherichia coli K88, Escherichia coli K99 and staphylococcus aureus, high cellulase producing capacity and ability of degrading cellulose. The biological feed additive prepared by using the bacillus subtilis provided by the invention can be used in place of part of antibiotics in livestock and aquatic product culture, improve immunity in animal, improve feed conversion rate and lower culture cost. The bacillus subtilis also can be used in fermentation of bean pulp, cotton meal, vegetable mealand the like, prevent the feed from mildewing, promote the digestion of cellulose in feed and improve the utilization rate of nutrients in the feed.

The invention relates to a high-yield culture technique of wheat, including land preparation, seeding, fertilizer application, watering and disease and insect control; the high-yield culture technique is characterized in that: the pattern of double sowing in one ridge with equal row distance and band seeding with broad width is adopted for sowing, and a wheat seeder with broad width is used for sowing. The high-yield culture technique has the advantages that: as the land occupying space of a single plant of wheat is enlarged, the leaves of wheat are not crowed, thus chemical weed killing effect can be greatly improved; the enlargement of the growing area of a single plant is beneficial to a robust individual and good lodging resistant effect; on the basis of fine-seeding, tillering is increased, the development of colony is reasonable, grains are not crowded, the growth state of competing for fertilizer, water and nutrition does not exist among individuals, the wheat has robust growth and strong adverse resistance, the high-yield culture technique is beneficial to large spike cultivars to form more spikes, and multi spike cultivars to form large spikes, thus facilitating larger spikes and more grains and obviously improving yield. The enlargement of the nutrition adsorption area of an individual plant is good for the development of the root system and the enhancement of vitality, and the late green functional period of wheat is prolonged, which can relatively delay the aging time of wheat and is beneficial to increasing wheat grain weight.

The invention provides bacillus subtilis K018 which is strong in stress resistance, excellent in probiotics function and safe and reliable, a biological feed additive containing the bacillus subtilis K018 and use thereof. The bacillus subtilis K018 provided by the invention can resist artificial gastric acid, cholate, artificial intestinal juice and high temperature, has stronger inhibiting effects to pathogenic staphylococcus aureus, escherichia coli, salmonella enteritidis and the like in the intestinal tract, is stronger in amylase and cellulase generating capacity, and can degrade starch and cellulose.

The invention provides a composite amino acid ecological nutritious agent, which comprises sea weed fermentation liquor, fermentation liquor of animal leftover bits and pieces, trace elements, and biological bacterium. The functions of the agent include stimulating growth, suppressing pathogenic agent and reinforcing immunization.

The invention relates to the field of deodorization, and especially relates to a plant extract and microbe composite deodorant. According to the invention, a plant extract and a microbial fermentation broth are mixed according to a ratio of 1:3; the mixture is placed in plastic buckets; and sealed anaerobic fermentation is carried out for 5-9 days, such that the composite deodorant is obtained. The microbial fermentation broth is obtained through the following steps: saccharomycetes, lactobacillus, bacillus subtilis, acetobacter pasteurianus and photosynthetic bacteria are inoculated according to the ratio of 1-4:2-6:5-25:1-4:1-2, and mixed fermentation is carried out; and after 60-84h of aerobic fermentation, the microbial fermentation broth is obtained. The invention aims at providing the plant extract and microbe composite deodorant. Deodorizing is realized with the combination of the plant extract and microbes. With the deodorant, fast and continuous deodorization can be realized; production cost is reduced; and a deodorization effect is good. With the deodorant, deodorization can be achieved fundamentally.

The invention relates to a compound microbial organic fertilizer and a preparation method thereof. The compound microbial organic fertilizer is prepared from the following ingredients in parts by weight: 1-4 parts of trichoderma viride JUWEI002 fermentation liquor, 1-4 parts of bacillus subtilis JUWEI001 fermentation liquor, 20-30 parts of turfy soil, 33-38 parts of tobacco residue, 33-38 parts of silkworm faeces, 6-7 parts of calcium powder and 1-2 parts of potassium fulvate. The compound microbial organic fertilizer disclosed by the invention has the advantages that the quality of crops can be remarkably improved, the yield of the crops is increased, and the soil structure is improved.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The invention discloses a high-yield cultivation method for kudzu roots, belonging to the technical field of crop cultivation. The high-yield cultivation method is characterized by mainly comprising the steps: cultivating kudzu seedlings; planting the kudzu seedlings; performing field management; harvesting the kudzu roots; reserving seeds for planting and the like. A cuttage breeding method is adopted for cultivating the kudzu seedlings; 1,000-1,300 kudzu seedlings are planted per mu during planting of the kudzu seedlings; the field management is performed with shelving, trimming and nutrient and water management as the core. Compared with the prior art, the high-yield cultivation method has the advantages that an intensive cultivation method is adopted, so that the kudzu seedlings grow strongly and have high stress resistance, the field management such as shelving and trimming is adopted, so that the illumination area of kudzu vines is increased, the photosynthesis is enhanced, meanwhile, the application of a root basal fertilizer and the application of a foliar fertilizer are of the same importance through a scientific fertilization method, the synthesis of kudzu rootstarch is promoted, and the yield is improved. The cultivation method is easy to operate and convenient to implement and has a good economic benefit.

The invention discloses a method for cultivating spring corn in hilly region wherein breeds, seeding date, density, mulch film blanketing and mulch film nursery sock grow transplanting, fertilizer application are considered for increasing maize production, experimental investigation and demonstration application are conducted to spread the fine breeds suitable for the Wuling Mountains which have the advantages of late ripening, medium late ripening, big ear, medium-sized to large ear.

The invention relates to a flower special fertilizer containing rare earth, which belongs to the fertilizer field; the components thereof are R1, R2, R3, RE and auxiliary materials, wherein, R1 is at least two kinds of major elements such as N, P and K which are necessary for flower growth, R2 is at least one of medium elements such as Ca, Mg and S, R3 is at least one of microelements such as Zn, B and Fe and RE is at least one of rare earth elements such as La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Y and Sc. According to the different weight proportions of R1, R2, R3, RE and various elements therein, a base-application special fertilizer or a rush-application special fertilizer for flowers of ornamental flower, ornamental foliage and ornamental fruit is prepared. The flower special fertilizer has balanced nutrition and reasonable proportion, can remarkably promote flower growth and improve the stress resistance, avoids flower quality reduction caused by nutrient deficiency, and reduces nutrient losses, avoids environmental pollution and is used for flower production at the same time.

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses an antibiotic Bacillus amyloliquefaciens with powerful bacteriostasis and wide bactericidal spectrum. The strain has been preserved in the China General Microbiological Culture Collection Center and the taxonomy name of the strain is: Bacillus amyloliquefaciens with preservation number CGMCCNo.4777. The antibiotic Bacillus amyloliquefaciens has powerful bacteriostasis, wide bactericidal spectrum, high inhibition rate up to above 93.5% on the rice blast fungus, the Botrytis cinerea and the Phytophthora parasitica; the Bacillus amyloliquefaciens also has very strong stress resistance, and very strong resistance to the acid and alkali, and is suitable to be used as microbial bactericide.

The present invention relates to fertilizer preparing technology, and is especially one kind of foliage fertilizer containing amino acid and with fast acting and its preparation process. The foliage fertilizer includes potassium dihydrogen phosphate 2-4 weight portions, copper sulfate 0.3-0.6 weight portions, ferrous sulfat 0.4-0.8 weight portions, magnesium sulfate 0.4-0.8 weight portions, zinc sulfate 0.3-0.6 weight portions, borax 0.4-0.8 weight portions, manganese sulfate 0.2-0.4 weight portions, calcium sulfate 0.2-0.4 weight portions, urea 2-4 weight portions, honey 0.1-0.3 weight portions, milk 0.5-1.0 weight portions, water soluble vitamins electrolyte 0.02-0.04 weight portions, amino acid 0.5-1.5 weight portions, fish liver oil 0.02-0.04 weight portions, vitamins B 0.04-0.12 weight portions. The foliage fertilizer has rich nutrients and pH 5.5-6.5 after being diluted, is suitable for foliage absorption and may be used to replace root applying organic fertilizer.

The invention discloses a breeding method of colorful rose and Chinese rose miniascape, which is characterized by the following: grafting roses and Chinese roses with different colors on the wild rose stock; making stock as wild rose stump; cultivating in the winter; surviving to shape; nourishing root; breeding stem; reshaping; grafting; naming the rose as Rcathayensisvar, Platphyllahu.

The invention provides a preparation method of a special biofertilizer for tobaccos. The special biofertilizer is prepared by effectively mixing raw materials including a multi-element biological agent, a cake fertilizer, a silkworm fertilizer, chitosan, attapulgite powder, humic acid and livestock / poultry excrement in certain conditions. The special biofertilizer has good effects of stable performance, lasting fertilizer efficiency and strong stress resistance, remarkably increases the yield and obviously improves the equality; and meanwhile, the raw materials are easily available, and the production cost is low.

The invention relates to a composite chilo suppressalis attractant luring female and male moths of chilo suppressalis simultaneously and application of the composite attractant, and discloses a chilo suppressalis attractant, and application of the chilo suppressalis attractant. The chilo suppressalis attractant is composed of a sex attractant and a host smell composition. The host smell composition comprises, by weight, 2-4 parts of limonene, 12-18 parts of zingiberene, 12-18 parts of caryophyllene and 60-74 parts of trans-3-Propyalacrolein. The synergistic interaction of the luring effect is achieved through combined usage of the chilo suppressalis sex attractant and the host smell composition. The chilo suppressalis attractant can be accurately applied to chilo suppressalis situation forecasting, mass trapping and mating interfering, and very important significance is achieved for comprehensive treatment of the chilo suppressalis.

The invention belongs to the field of biotechnology, and relates to Lactobacillus acidophilus and application thereof and a feed additive thereof and a premix compound thereof. The Lactobacillus acidophilus of the invention has strong stress tolerance, and can tolerate simulated gastric fluid and simulated bile salt so as to guarantee that a great quantity of viable bacteria can smoothly enter the intestinal tract of an animal, and the viable bacteria play a probiotic role, so that the immunity of the animal is improved, and the breeding cost is reduced. The product obtained from the Lactobacillus acidophilus of the invention through culture, high-density fermentation and addition of protectant has a high content of viable Lactobacillus acidophilus, can be added as a biological feed additive in the drinking water or feed for breeding animals, and can be prepared into feed such as premix compound and so on, so as to reduce the addition of antibiotics, indeed improve the intestinal tract health and the immunity of animals, and increase the production performance of animals.

The invention discloses a separation and culture method using ginseng cambium stem cells. The separation and culture method using ginseng cambium stem cells is characterized by comprising the following steps: (1) firstly performing disinfection treatment on the root tubers with the diameter of 2 cm of northeast ginseng by 0.1% mercury bichloride for 10 minutes, and then transversely cutting the northeast ginseng into semicircular slices with the thickness of 0.2 cm and containing periderms, phloems and cambiums; (2) placing the semicircular slices on a WPM (woody plant medium) containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and culturing at 25 DEG C in the dark; (3) taking out the obviously-multiplied explants of the cambiums after two weeks, separating the cambium stem cells, transferring the cambium stem cells on a subculture medium and culturing, wherein the subculture medium is a WPM containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and subculture is performed every two weeks. According to the method, separation and culture for ginseng stem cells are performed by using the unsplit cambium stem cells, and the cambium stem cells are infinitely multiplied, so that production can be effectively expanded.

The invention relates to the technical field of marine product seed breeding and in particular relates to an industrialized iconological breeding method of young shrimps having high stress resistance and high disease resistance. The industrialized iconological breeding method comprises the steps of the construction and sterilization of an isolating breeding workshop and a standard breeding pool, the integrated treatment of breeding water, the healthy cultivation and reproduction of prawn parents, the throwing-in of healthy nauplius, the preparation of a breeding bait, the biological control and management of breeding water quality, bait feeding management, control of physical factors such as temperature and light, and the like. Due to biological control on the water environment of the indoor breeding pool and effective control on water temperature, illumination, transparency, dissolved oxygen, salinity, PH value, harmful substances, pests and the like, the breeding environmental conditions are optimized, the drug use, the feeding capacity and the drainage are reduced; in addition, the stress resistance and the disease resistance of the young prawns are enhanced, the quality and the survival rate of the young prawns are increased. The industrialized iconological breeding method of young prawns having high stress resistance and high disease resistance is used for solving the key technical problems of prawn breeding; a high-efficiency environment-friendly breeding mode is provided to promote efficient sustainable development of the prawn breeding industry.

The invention provides a method for cultivating a novel Dingyuan pig breed, which relates to a method for cultivating a novel pig breed. The invention provides the method for cultivating the novel Dingyuan pig breed which has the advantages high fecundity, good meat quality and strong stress resistance of Dingyuan pigs as well as the characteristics of fast growth, high lean meat percentage and the like of imported pigs. The method comprises the following steps: a Dingyuan sow is hybridized with a novel USA strain Duroc boar to cultivate a first generation sow core group; second grading hybridization is performed to the first generation sow core group and a Dingyuan boar or the novel USA strain Duroc boar; from the third generation, group closure propagation and breeding are adopted respectively, and crossing of two to three generations is performed; and selection and remaining are performed through methods of comprehensive selection indexes and molecule marking assistance selection to form the novel Dingyuan pig breed, namely a novel breed of Wan Huai pigs and a novel breed of Dingyuan-Duroc pigs.

The invention discloses a method for planting organic vegetable okra. The method mainly comprises the steps of seed processing, soil preparation and fertilization, seedling transplantation, field management and harvesting. During the middle term of the okra, intertillage and earthing up need performing in time, and the soil is topdressed with organic fertilizers containing amino acid and humic acid. During insect control, natural source levorotation terpilenol and S-S rosin insecticides are utilized, diseased leaves, plant residues and weeds are processed through an organic method, and the whole process is free of pollution caused by chemicals. The organic planting model of the okra provides a firm technical foundation for promoting large-scale and standardized production of the organic vegetable okra planting industry.

The invention discloses a method for preparing biological compound fertilizer, which comprises steps of preparing an organic adhesive, preparing fertilizer core, preparing a coating agent, coating and the like. In the method, earthworm manure and humic acid are used as organic components, a NPK medical fertilizer is used as an inorganic component, rich microbes and enzymes in the earthworm manure and added released phosphorus and potassium are used as a microbial component, modified starch is used an organic adhesive for granulating the fertilizer, and natural cellulose such as grain bran is used as a modified polymer water absorbing agent for making a coating material. The prepared fertilizer integrates inorganic and organic components and microbes, and is a biological compound fertilizer with multiple functions of water retaining, sustained release, stress resistance and absorption promotion.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention discloses bacillus pumilus, a method for acquiring the strain, and application of the strain in orientated degradation on nitrosoamine specifically in tobacco, and belongs to the technical field of microorganism application. The bacillus pumilus 05-5402 is preserved in the Common Microorganism Center of the Chinese Microorganism Strain Preservation Administration Committee, and the preservation number is CGMCC No. 7418. The method for acquiring the strain comprises procedures of separation, screening and purification, namely, firstly separating a strain which can degrade nicotine from tobacco plant or soil, further screening by using a culture medium which takes NNK (Tobacco-Specific Nitrosamine) as a sole carbon source and nitrogen source, and finally purifying on a culture medium plate so as to obtain the bacillus pumilus. The application of the bacillus pumilus comprises procedures of fermentation, inoculation and degradation, namely, firstly culturing the strain for 48-72 hours at 25-30 DEG C so as to obtain a zymophyte agent, spraying the zymophyte agent according to a percentage of 3-5% of the weight of the tobacco in the tobacco leaf conditioning or slicing process, and keeping the degradation time of 4-7 days. Through the adoption of the strain, high-efficient orientated degradation on nitrosoamine specifically in tobacco is achieved, and the strain is good in stress resistance, simple and convenient to obtain and convenient to use, and has high popularization and application values.

The invention provides a colorful rose and Chinese rose bonsai cultivation method. According to the method, roses and Chinese roses of various colors and different varieties are adopted as scions and grafted to a protospecies stock of a wild rose.