24 results about "Stem Cell Isolation" patented technology

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

The preparation method comprises the following steps: digesting and collecting stem cells, separating high-purity mitochondria for later use, dropwise adding a prepared beta-sodium glycerophosphate solution into a chitosan solution to prepare blank gel for later use, mixing the mitochondria stock solution with the blank gel, and carrying out freeze drying to obtain the mitochondria-loaded gel preparation. The gel preparation loaded with the mitochondria is prepared. According to the invention, the stability of mitochondria in an in-vitro environment can be maintained, the mitochondrial transplantation efficiency and the treatment effect are improved, and technical support is provided for mitochondrial transplantation treatment of mitochondrial dysfunction related diseases.

The present invention relates to: a stem cell isolation kit manufacturing composition prepared with graphene dispersed therein, and a preparation method therefor, the composition enabling cell activity of stem cells to improve and blood clots to be prevented through mixing of graphene and a thermoplastic resin. The stem cell isolation kit manufactured using the stem cell isolation kit manufacturing composition comprising graphene, of the present invention, allows autologous stem cells to be activated by an environment in which biocompatible far-infrared (FIR) emission and electrical conductivity are increased, and, under hypoxic conditions, enables stem cell water amount to be increased through low oxygen permeability (water activity). In addition, the present invention is subjected to antibacterial treatment so as to provide an antibacterial effect, and can improve a platelet adsorption reduction rate through a water slip effect of the graphene composition.

The utility model relates to the technical field of biological experiment instruments, in particular to a hanging accessory used in the separation process of umbilical cord blood hematopoietic stem cells, which is mainly used for being connected with a grid at the top of a biological safety cabinet and comprises a fixed end connected with the grid at the top of the biological safety cabinet, and a hook used for being connected with a blood bag is arranged below the fixed end. The fixing end comprises a clamping ring, a torsional spring clamping opening capable of being clamped into a grid is formed in the clamping ring, a limiting sliding groove is formed in the inner side of the clamping ring, and a limiting rod capable of moving up and down along the inner wall of the clamping ring is slidably connected into the limiting sliding groove. A reset spring capable of resetting the limiting rod upwards is arranged between the bottom of the limiting sliding groove and the limiting rod. The technical problems that in the prior art, a blood bag is connected with a grid in a biological safety cabinet through a paper clip, then the blood bag is hung, shaking is large, the blood bag is prone to falling off, a sample possibly needs to be centrifuged many times, and the motility rate of the sample is affected are solved.

The invention discloses a separation device for human stem cell culture, and relates to the technical field of stem cell culture, the separation device comprises a case, a hinge is fixedly arranged on the upper surface of the case, and a cover plate is fixedly arranged at the movable end of the hinge. By arranging the machine box, a closed and clean operation cavity is provided for the whole stem cell separation device, pollution of the external environment to a culture sample is effectively isolated, and meanwhile the machine box serves as an integrated bearing platform of all functional parts. The upper cover structure capable of being opened and closed is formed by arranging the hinge and the cover plate, and the temperature adjusting mechanism is used for adjusting the temperature of the test tube. The temperature adjusting mechanism is arranged and is a key system for ensuring that a suitable temperature environment is maintained in the separation operation process of a stem cell culture sample, and a stable and uniform local temperature field is provided for each test tube through active heating and directional airflow conveying. The effect of keeping the temperature of the test tube stable during stem cell separation is achieved.

The utility model relates to the technical field of kits, and provides a mesenchymal stem cell separation and amplification kit which comprises a kit body, a cover plate is arranged at the top of the kit body, a driving piece is arranged at the bottom in the kit body, a rectangular block is fixedly connected to the top end of the driving piece, and driving assemblies are arranged on the four side faces of the rectangular block; according to the mesenchymal stem cell separation and amplification kit disclosed by the utility model, the driving component can rotate by arranging the driving piece, so that the driving component collides with the extrusion block and the ball on the inner wall of the kit; under the matching action of a connecting rod, a rectangular rod, a hollow rod, a rectangular groove, a spring, a rectangular frame, a test tube sleeve, an extrusion plate, a spring I and a collision block, cell tissue fluid in a reagent tube can shake up and down and left and right by virtue of the restoring force of the spring and the spring I, so that the cell tissue fluid is never effectively prevented from precipitating.

The invention relates to the technical field of stem cell separation, in particular to an umbilical cord blood hematopoietic stem cell device convenient for efficient separation, which comprises a rotating disc, an outer cylinder is fixedly connected to the upper surface of the rotating disc, a separation cylinder is arranged in the outer cylinder, and a driving module is arranged below the rotating disc. A self-adaptive clamping module is arranged at the inner bottom of the outer cylinder, a displacement opening is formed in the outer surface of the outer cylinder, a positioning module is arranged in the displacement opening, a communicating pipe is connected to the outer side of the bottom of the separation cylinder, a liquid level shell is connected to the interior of the communicating pipe, and a servo motor is matched with a first ball bearing and a driving shaft; through cooperation of the annular balance groove and the spherical balance column, stable and controllable centrifugal power can be provided for the rotating disc, the balance weight effect of the centrifugal block on the outer side of the outer barrel is matched, it is ensured that the separation barrel can still keep high-speed rotation stability when the sample size of 50-200 ml fluctuates, and the problem that in the centrifugal process, due to equipment shaking, a layered interface deviates is solved.

The present invention addresses the problem of providing an MSC which is HLA-ABC negative and HLA-G positive.　The problem can be solved by a mesenchymal stem cell isolated from a placenta, said mesenchymal stem cell being HLA-ABC negative and HLA-G positive.

The utility model discloses a mesenchymal stem cell separates with centrifugal device relates to centrifugal device technical field, including centrifuge body, the inside bottom swing installation of centrifuge body has the connecting mechanism, the upside swing installation of connecting mechanism has the rack, the upside fixed connection of rack has the fixed mechanism, the outside swing installation of centrifuge body has the damping mechanism, the fixed mechanism includes the mechanism board, the inside rotation of mechanism board is connected with a plurality of even distribution's fixed group. A mesenchymal stem cell separates with centrifugal device, by rotating gear ring, cooperation second gear, make multiple rotating disc synchronous rotation, and then make the clamping rod mutual approach and carry out the clamping of test tube to the test tube in the inside middle part of fixed disc, simultaneously to the test tube multidirectional force clamping, make the test tube vertical in fixed disc, and then make the fixation of test tube more stable.

The invention provides a construction method of a thyroid organoid derived from human induced pluripotent stem cells, and belongs to the technical field of organoid culture. The construction method of the thyroid organ comprises the following steps: (1) separating human induced pluripotent stem cells into single cells, and transferring the single cells to a 2D matrigel working solution for incubation; (2) carrying out endoderm induced culture on the cells incubated in the step (1); (3) carrying out preintestinal endoderm induced culture on the cells obtained in the step (2); (4) carrying out thyroid gland directional induction culture on the cells obtained in the step (3); (5) carrying out thyroid maturation induction culture on the cells obtained in the step (4) to obtain thyroid organs; naI with the final concentration of 0.8-1.2 [mu] M is added during thyroid maturation induction culture. The thyroid organoid constructed by the method is high in maturity, can simulate the state of the in-vivo organoid more truly, and has good capability of secreting thyroid-related hormones at the same time.

The utility model relates to the technical field of dental pulp stem cell separation, and discloses a dental pulp stem cell separation and extraction device which comprises a device shell, a driving shaft is installed in the device shell, and a stepping motor fixedly connected with the device shell is fixed to the lower end of the driving shaft. A balance mechanism used for balancing the device is arranged on the outer surface of the driving shaft, a fixing mechanism used for fixing materials is arranged at the upper end of the driving shaft, and a protection mechanism used for protection is arranged on one side of the device shell. The balance mechanism comprises an adjusting piece. Through the arrangement of the balance mechanism, the lead screw can be driven to rotate through the adjusting motor arranged in the adjusting piece, the horizontal position of the movable block can be adjusted under the cooperation of the guide rod, then the position of the balancing weight is adjusted, the centrifugal force generated when the driving shaft rotates can be stabilized, and then the shaking situation during centrifugation is reduced; and the stability of the device is improved.

The utility model relates to the technical field of regenerative medicine and cell therapy, in particular to a device for mechanically extracting stem cells, which comprises a hollow tube for storing tissues and a shearing plate provided with shearing holes, the shearing plate is arranged in the hollow tube and divides the hollow tube into a first chamber and a second chamber which are independent from each other, and the first chamber and the second chamber are communicated with each other. The first cavity and the second cavity are communicated through shearing holes in the shearing plate, a pushing mechanism used for organizing circulation between the first cavity and the second cavity is further arranged in the hollow pipe, two material pipes are arranged on the hollow pipe, the two material pipes are communicated with the first cavity and the second cavity respectively, and valves are arranged on the material pipes. The device for mechanically extracting the stem cells realizes homogenization treatment of tissues and efficient extraction of the stem cells through physical shearing, extrusion and bidirectional mechanical action, completely avoids time consumption of a traditional enzyme digestion method, reduces dependence on collagenase or other chemical reagents, and improves the extraction efficiency of the stem cells. The method is suitable for stem cell separation of tissues such as umbilical cord and fat with low damage and without allogenic material residue.

The invention belongs to the technical field of in-vitro culture of germline stem cells, and particularly relates to a separation and in-vitro culture method of spermatogonial stem cells of grass carp. The method comprises the following steps: by taking male grass carps as donors, anesthetizing, killing, cleaning, disinfecting, dissecting and separating gonad tissues, crushing into fragments by adopting a mode of combining magnetic bead crushing with shearing, digesting with trypsin, filtering and centrifuging to obtain high-purity spermatogonial stem cells, and performing in-vitro culture on the spermatogonial stem cells by adopting a culture solution. By optimizing the age of the male grass carp, an enzyme digestion process and an in-vitro culture system and adopting a one-step enzyme digestion method, the digestion time is shortened, the operation process is simplified, and the industrial problems that the spermatogonial stem cells of the grass carp are low in separation efficiency and culture is easy to differentiate are solved. According to the invention, a separation, purification and primary culture technical system of the spermatogonial stem cells of the grass carp is systematically established for the first time, and important cell materials and technical supports are provided for breeding technologies such as transplantation of germline stem cells of the grass carp, gene editing and the like.

This invention relates to the field of stem cell isolation, and discloses a dental pulp stem cell isolation reactor and a method for efficient exosome extraction. The reactor includes a controller, a protective shell and a motor mounted on the inner wall of the controller, a rotating frame fixedly connected to the output end of the motor, a test tube slidably connected to the bottom end of the placement frame, a fixing component on the inner wall of the placement frame, toothed rings fixedly connected to both sides of the placement frame, a movable frame threadedly connected to the outer wall of the threaded rod, a rack fixedly connected to the bottom end of the movable frame, a synchronous belt meshing with the toothed end of a first synchronous wheel, and a second synchronous wheel fixedly connected to one end of the rotating wheel. By placing a test tube containing dental pulp stem cells into the placement frame and fixing it, rotating the rotating wheel causes the placement frame to rotate within the rotating frame via a transmission component to adjust the angle of the test tube. After covering with a movable cap, the controller controls the motor to drive the rotating frame to rotate, thereby changing the centrifugal force on the stem cells.

The utility model discloses a stem cell separation and detection device, which relates to the technical field of stem cell separation and detection devices and comprises a base plate, a centrifugal machine, a flow cytometer, a mounting rack and a mounting rod, the centrifugal machine and the flow cytometer are both fixedly mounted at the upper end of the base plate, and a centrifugal cylinder is arranged on the inner side of the upper end of the centrifugal machine. A supporting rod is fixedly mounted at the upper end of the centrifugal machine, a hook is arranged at the upper end of the supporting rod, and the mounting rod is arranged on the front face of the supporting rod and arranged on the back face of the supporting rod. By the adoption of the structure, after the installation rod and the installation frame are connected at the position of the supporting rod, the clamping block is pushed to slide through rotation of the first hand-screwing bolt and threaded telescopic rotation of the first hand-screwing bolt, and due to the fact that the opposite faces of the clamping block and the installation rod are provided with the V-shaped grooves, the clamping block can be rapidly installed and fixed on the supporting rod conveniently; and the operation can be carried out without a tool, so that the operation is simple and convenient.

This invention provides a method for isolating spermatogonial stem cells from frozen testicular tissue, belonging to the field of cell biology. The isolation method provided by this invention includes the following steps: thawing frozen testicular tissue; digesting the thawed tissue with collagenase IV and DNase I until the seminiferous tubules are exposed and their walls become rough; washing with DPBS buffer, centrifuging, and culturing until cells migrate; collecting the migrated cells and purifying them for spermatogonial stem cell enrichment. The method described in this invention can maximally preserve the internal environment of spermatogonial stem cells, maintain cell viability, and reduce cell damage, and can be used for isolating spermatogonial stem cells from frozen testicular tissue.

The present invention relates to: a composition for manufacturing a stem cell isolation kit manufactured by dispersing graphene, the composition being capable of maintaining the moisture content of stem cells by mixing graphene and a thermoplastic resin; and a manufacturing method therefor. The stem cell isolation kit manufactured from the composition for manufacturing a stem cell isolation kit comprising graphene of the present invention is configured such that autologous stem cells are activated by biocompatible far-infrared (FIR) emission and an environment with increased electrical conductivity, and is further configured to improve the moisture content (water amount) of stem cells due to low oxygen permeability (water activity) under hypoxic conditions (hypoxia). In addition, the kit exhibits an antibacterial effect through antibacterial treatment, and can improve a reduction rate of platelet adsorption due to the water slip effect of the graphene composition.

The present disclosure relates to potency assays for cell therapy products. To provide a potency assay of a cell population comprising mesenchymal progenitor or stem cells.SOLUTION: The present invention relates to a method of measuring the biological activity or therapeutic efficacy of cultured mesenchymal lineage precursor or stem cells based on the levels of TGF-9 they release during culture. The invention also relates to an isolated population of mesenchymal lineage precursor or stem cells selected on the basis of the level of TGF-9 levels that the cells release in culture. The invention further relates to treating a subject suffering from degenerative disc disease by administering a cell population so selected.SELECTED DRAWING: None

The application discloses a kind of digestive juice for separating gastric corpus stem cell and its use method, belong to stem cell separation technical field;The digestive juice includes the following concentration components:18.5~28 nM Na2HPO4, 40~48 nM KH2PO4, 460.5~530 nM NaCl, 1~15.2 nM KCl, 200~350.55 nM D-furan fructosyl-D-glucopyranoside, 400~605 nM D-glucitol, 2~4 nM ethylenediaminetetraacetic acid disodium and 2~3 nM 1,4-dithiothreitol.The application also discloses the use method of the above digestive juice.The digestive juice for separating gastric corpus stem cell and its use method of the application can solve the problems of low cell yield, low activity, insufficient purity and poor repeatability of existing digestive juice and supporting use method, and have wide application prospect.

The present application relates to a kind of mouse palatine midline stem cell separation and culture method, belong to stem cell separation culture technical field.The method of the present application includes the following steps: S1: separation 3-5 days old mouse maxillary tissue, retain palatine midline and its two sides tissue, tear two sides tissue along palatine midline, obtain unilateral palatine midline tissue;S2: with the medium containing serum, antibiotic and growth factor is cultured within 7 days in step S1 unilateral palatine midline tissue, obtain primary mouse palatine midline stem cell;The growth factor includes at least one of epidermal cell growth factor, fibroblast growth factor and transforming growth factor;S3: step S2 primary mouse palatine midline stem cell is subcultured, and obtain subculture mouse palatine midline stem cell.The method of the present application greatly shortens experimental time, and the palatine midline stem cell extracted is high, and it is convenient to operate, and it provides basis for the repair of cleft palate and other congenital developmental deformity.

This utility model relates to the field of cell separation and extraction technology, and provides a mesenchymal stem cell separation and extraction device, including a box and a second rotating wheel. A door is provided on the side wall of the box, and a control center is provided on the surface of the door. A placement plate is fixedly connected to the inner side wall of the box, and a temperature sensor is provided on the side wall of the box. A rotating block abuts against the upper surface of the placement plate. A cooler is provided on the top wall of the box, and a partition is fixedly connected to the lower side wall of the box near the cooler. This utility model's mesenchymal stem cell separation and extraction device solves the technical problems of existing stem cell separation and extraction devices lacking a function to fix reagent bottles, causing the reagent bottles containing stem cells to shake during separation, and existing stem cell separation and extraction devices lacking a cooling function, which easily leads to stem cell death when separating stem cells at high temperatures.

The invention discloses a stem cell extraction process. The stem cell extraction process comprises the following steps: step 1, pretreating adipose tissues; step 2, dissolving fat particles, adding collagenase with the same amount into the fat tissue in the first centrifugal test tube, and carrying out water bath digestion; step 3, primary centrifugation: putting the plurality of first centrifugal test tubes into a centrifugal machine for centrifugation; step 4, primary extraction of supernate: placing the plurality of first centrifugal test tubes on a supernate extraction device to remove residues of the supernate; step 5, secondary centrifugation: placing a plurality of first centrifugal test tubes in an open state on a test tube fixing cover, connecting the test tube fixing cover with a second centrifugal test tube, and performing centrifugal processing on the second centrifugal test tube; and 6, secondary extraction of the supernatant: removing the supernatant in the second centrifugal test tube by using the supernatant extraction device to obtain the adipose-derived stem cells. The stem cell extraction process provided by the invention is convenient for accurate and efficient removal of the supernate during stem cell separation.

This invention discloses a layered filtration constant-temperature oscillation device for hematopoietic stem cell isolation and culture, comprising a horizontally placed support frame, a control console fixedly mounted on the bottom upper surface of the support frame, a mounting frame disposed on the upper front side of the support frame, and two filter cylinders fixedly mounted inside the mounting frame, with a filter membrane fixedly mounted inside the lower end of each filter cylinder. The mounting frame is connected to the support frame via an oscillation mechanism. A collection tank is disposed above the control console and is connected to the bottom of the filter cylinders via a connecting pipe. A heat exchange pipe for constant-temperature heating of the culture solution is fixedly mounted inside each filter cylinder. This layered filtration constant-temperature oscillation device for hematopoietic stem cell isolation and culture employs a novel structural design. A blower mechanism supplies air to a nozzle through a delivery pipe, and the air is finally ejected from the nozzle. The ejected air is then delivered to the filter cylinders through a pressurization pipe, pressurizing the filter cylinders and accelerating the filtration and separation speed.