55 results about "Stem Cell Isolation" patented technology

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention discloses a method for screening an active matter for protecting skin cell telomeres, and relates to the technical field of biotechnology and cosmetic development, and the method is technically characterized by comprising the following steps: a) providing stem cells derived from an exosome which carries at least one microRNA for regulating and controlling a telomere protection factor CTC1; b) acting a candidate active matter on the stem cells, and separating to obtain a treated exosome; the technical effects are as follows: the combination of miR-150-5p and miR-181c is delivered through the exosome, the dual-miRNA coordinated regulation is realized, the limitation of the traditional single-target detection is broken through, the CD63 / CD81 protein on the membrane surface of the exosome can be specifically combined with the skin cell membrane, and the Franz diffusion experiment verifies that the micro-fluidic chip integrates the collagen scaffold and the dynamic shear force.

The invention aims to provide a method for effectively separating and proliferating stem cells from peripheral blood. The composition according to the present invention comprises interferon-gamma (IFN-gamma) in order to enhance the adhesion of stem cells isolated from peripheral blood and thus improve the acquisition rate of stem cells. Therefore, the composition and the peripheral blood-derived stem cell isolation and culture method according to the present invention have been established for inducing progenitor cells differentiated into somatic cells that can be differentiated into various target tissues, and for preventing or treating diseases.

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

The invention relates to the technical field of organoids, and particularly discloses a chick intestinal tract crypt stem cell separation medium and a separation method and application thereof, and the technical key points are as follows: the separation medium provided by the scheme of the invention can efficiently separate chick intestinal tract crypts with complete structure, good activity and high purity under a low-temperature condition by optimizing component combination; according to the separation method provided by the scheme of the invention, the separation efficiency and the culture quality of the intestinal stem cells and the intestinal organs are remarkably improved through the matching of the separation liquid and the washing liquid. By utilizing the method disclosed by the invention, a high-quality stem cell source can be provided for culture of intestinal organs, drug screening and disease model construction, and the method has important scientific research and application values.

The preparation method comprises the following steps: digesting and collecting stem cells, separating high-purity mitochondria for later use, dropwise adding a prepared beta-sodium glycerophosphate solution into a chitosan solution to prepare blank gel for later use, mixing the mitochondria stock solution with the blank gel, and carrying out freeze drying to obtain the mitochondria-loaded gel preparation. The gel preparation loaded with the mitochondria is prepared. According to the invention, the stability of mitochondria in an in-vitro environment can be maintained, the mitochondrial transplantation efficiency and the treatment effect are improved, and technical support is provided for mitochondrial transplantation treatment of mitochondrial dysfunction related diseases.

The present invention relates to: a stem cell isolation kit manufacturing composition prepared with graphene dispersed therein, and a preparation method therefor, the composition enabling cell activity of stem cells to improve and blood clots to be prevented through mixing of graphene and a thermoplastic resin. The stem cell isolation kit manufactured using the stem cell isolation kit manufacturing composition comprising graphene, of the present invention, allows autologous stem cells to be activated by an environment in which biocompatible far-infrared (FIR) emission and electrical conductivity are increased, and, under hypoxic conditions, enables stem cell water amount to be increased through low oxygen permeability (water activity). In addition, the present invention is subjected to antibacterial treatment so as to provide an antibacterial effect, and can improve a platelet adsorption reduction rate through a water slip effect of the graphene composition.

The utility model relates to the technical field of biological experiment instruments, in particular to a hanging accessory used in the separation process of umbilical cord blood hematopoietic stem cells, which is mainly used for being connected with a grid at the top of a biological safety cabinet and comprises a fixed end connected with the grid at the top of the biological safety cabinet, and a hook used for being connected with a blood bag is arranged below the fixed end. The fixing end comprises a clamping ring, a torsional spring clamping opening capable of being clamped into a grid is formed in the clamping ring, a limiting sliding groove is formed in the inner side of the clamping ring, and a limiting rod capable of moving up and down along the inner wall of the clamping ring is slidably connected into the limiting sliding groove. A reset spring capable of resetting the limiting rod upwards is arranged between the bottom of the limiting sliding groove and the limiting rod. The technical problems that in the prior art, a blood bag is connected with a grid in a biological safety cabinet through a paper clip, then the blood bag is hung, shaking is large, the blood bag is prone to falling off, a sample possibly needs to be centrifuged many times, and the motility rate of the sample is affected are solved.

The invention discloses a separation device for human stem cell culture, and relates to the technical field of stem cell culture, the separation device comprises a case, a hinge is fixedly arranged on the upper surface of the case, and a cover plate is fixedly arranged at the movable end of the hinge. By arranging the machine box, a closed and clean operation cavity is provided for the whole stem cell separation device, pollution of the external environment to a culture sample is effectively isolated, and meanwhile the machine box serves as an integrated bearing platform of all functional parts. The upper cover structure capable of being opened and closed is formed by arranging the hinge and the cover plate, and the temperature adjusting mechanism is used for adjusting the temperature of the test tube. The temperature adjusting mechanism is arranged and is a key system for ensuring that a suitable temperature environment is maintained in the separation operation process of a stem cell culture sample, and a stable and uniform local temperature field is provided for each test tube through active heating and directional airflow conveying. The effect of keeping the temperature of the test tube stable during stem cell separation is achieved.

The utility model relates to a mesenchymal stem cell separation and amplification kit which comprises a rectangular box body, a reagent bottle a, a reagent bottle b and a plurality of reagent tubes, the reagent bottle a, the reagent bottle b and the reagent tubes are arranged in the box body, the reagent bottle a is used for containing a culture solution, the reagent bottle b is used for containing a flushing agent, the reagent tubes are used for containing an inducer, and a separation and amplification culture box is further arranged in the box body. The whole separation and amplification culture box is in a rectangle shape matched with the size of a box body, a tissue access area is reserved in the center of the separation and amplification culture box, a plurality of amplification vertical plates extending from the center to the periphery in a radial mode are distributed on the periphery of the tissue access area in the circumferential direction, and the amplification vertical plates are of a trapezoidal structure gradually widened from inside to outside. Liquid injection and extraction grooves are formed in the two sides of the separation and amplification culture box. The mesenchymal stem cell separation and amplification kit integrates an amplification culture box adapting to the separation and amplification characteristics of mesenchymal stem cells, and a stepped complete separation and culture operation platform is formed after the box body is unfolded, so that the functionality of a product is enriched, and convenience is provided for separation and amplification practical operation of the mesenchymal stem cells.

The invention discloses a sheep female stem cell separation and in-vitro culture method, and relates to the technical field of biologication.According to the technical scheme, the sheep female stem cell separation and in-vitro culture method is characterized in that 0.25% pancreatin is adopted for digestion for 1 hour at the room temperature, mechanical cutting operation is combined, intercellular connexin can be effectively decomposed, and the survival rate of the sheep female stem cells is increased; the ovarian granular cells are quickly released. Medical alcohol and a culture medium containing double antibodies are used for cleaning ovaries for many times, so that the probability of bacterial and fungal contamination is remarkably reduced. A DPBS buffer solution preheated at 37 DEG C and a DMEM / F12 culture medium containing 10% of FBS are used in the whole process, and stress damage to cells caused by temperature shock is avoided. Complete granular cells and tissue fragments are efficiently separated through filtration and centrifugation, and the purity of a cell suspension is improved. The method is developed on the basis of ovaries of sheep, does not depend on feeder layer cells or a complex three-dimensional culture system, can be operated only by conventional laboratory equipment, and is suitable for research scenes with limited resources.

The invention relates to a method for separating, culturing and purifying human amniotic mesenchymal stem cells. The method comprises the following steps: collecting and pretreating a human amniotic membrane, washing, digesting pancreatin, neutralizing and washing, digesting type II collagenase, performing primary culture, performing passage purification and the like. Compared with the prior art, in the pancreatin digestion stage, by increasing the number of times in the pancreatin digestion process, mixing of hybrid cells can be greatly reduced, meanwhile, a large number of stem cells with good activity are obtained, and the extraction success rate of the stem cells is remarkably increased; due to the optimized operation process, tedious steps are reduced, and operability is improved; through multiple purification steps, it is ensured that the finally obtained cells have high purity and a good state.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The utility model relates to the technical field of kits, and provides a mesenchymal stem cell separation and amplification kit which comprises a kit body, a cover plate is arranged at the top of the kit body, a driving piece is arranged at the bottom in the kit body, a rectangular block is fixedly connected to the top end of the driving piece, and driving assemblies are arranged on the four side faces of the rectangular block; according to the mesenchymal stem cell separation and amplification kit disclosed by the utility model, the driving component can rotate by arranging the driving piece, so that the driving component collides with the extrusion block and the ball on the inner wall of the kit; under the matching action of a connecting rod, a rectangular rod, a hollow rod, a rectangular groove, a spring, a rectangular frame, a test tube sleeve, an extrusion plate, a spring I and a collision block, cell tissue fluid in a reagent tube can shake up and down and left and right by virtue of the restoring force of the spring and the spring I, so that the cell tissue fluid is never effectively prevented from precipitating.

The invention relates to the technical field of stem cell separation, in particular to an umbilical cord blood hematopoietic stem cell device convenient for efficient separation, which comprises a rotating disc, an outer cylinder is fixedly connected to the upper surface of the rotating disc, a separation cylinder is arranged in the outer cylinder, and a driving module is arranged below the rotating disc. A self-adaptive clamping module is arranged at the inner bottom of the outer cylinder, a displacement opening is formed in the outer surface of the outer cylinder, a positioning module is arranged in the displacement opening, a communicating pipe is connected to the outer side of the bottom of the separation cylinder, a liquid level shell is connected to the interior of the communicating pipe, and a servo motor is matched with a first ball bearing and a driving shaft; through cooperation of the annular balance groove and the spherical balance column, stable and controllable centrifugal power can be provided for the rotating disc, the balance weight effect of the centrifugal block on the outer side of the outer barrel is matched, it is ensured that the separation barrel can still keep high-speed rotation stability when the sample size of 50-200 ml fluctuates, and the problem that in the centrifugal process, due to equipment shaking, a layered interface deviates is solved.

The utility model relates to a mesenchymal stem cell separation device, which comprises a dish body, a dish cover and a liquid mechanism, the periphery of the bottom of the dish body extends downwards to form a lower edge, the bottom surface of the dish body sinks inwards to form a plurality of ridges, two sides of each ridge are abrupt slope surfaces, and the ridges are radially and circumferentially arranged on the bottom surface of the dish body. A V-shaped liquid permeable groove parallel to the ridges on the two sides is formed between every two adjacent ridges on the bottom surface of the dish body; the liquid injection mechanism comprises a liquid injection cavity with the lower portion closed up and a liquid permeating cavity with the upper portion unfolded, a liquid inlet connector and a liquid outlet connector are arranged on the liquid injection cavity, and a liquid injection pipe and a liquid outlet pipe are arranged on the lower portion of the liquid injection cavity. According to the mesenchymal stem cell separation device, the mode of ascending infiltration and descending emptying at the bottom is achieved, a culture solution is updated on the premise that the dish cover does not need to be opened and closed, meanwhile, a stem cell culture space is isolated from a liquid injection space and a liquid drainage space, damage to stem cells caused by injection and drainage of the culture solution is prevented, the dish cover does not need to be opened and closed in the culture solution updating process, and the culture solution updating efficiency is improved. The probability that the separated stem cells are polluted is effectively reduced.

The present invention relates to exosomes prepared from placenta mesenchymal stem cells and their uses. On the one hand, the present invention relates to exosomes isolated and extracted using placenta mesenchymal stem cells, which have an average particle size of 50-200 nm, and which express membrane protein CD9 and membrane protein CD81. The positive expression rate of the exosome membrane protein CD9 is greater than 65%, and the positive expression rate of the membrane protein CD81 is greater than 75%. The preparation method of the exosomes is as follows: inoculate mesenchymal stem cells into a culture flask, culture to make the cells adhere to the wall, and then add IL-1β to the culture medium for culture; after replacing the complete culture medium, culture in an incubator at 37°C, 2% O2, and 5% CO2; subject the cells to differential centrifugation to obtain exosomes in the form of a suspension. The present invention also relates to the use of the prepared exosomes in the preparation of drugs for treating inflammatory diseases. The method of the present invention has the advantages of high exosome yield, high activity, etc.

The present invention addresses the problem of providing an MSC which is HLA-ABC negative and HLA-G positive.　The problem can be solved by a mesenchymal stem cell isolated from a placenta, said mesenchymal stem cell being HLA-ABC negative and HLA-G positive.

The utility model discloses a mesenchymal stem cell separates with centrifugal device relates to centrifugal device technical field, including centrifuge body, the inside bottom swing installation of centrifuge body has the connecting mechanism, the upside swing installation of connecting mechanism has the rack, the upside fixed connection of rack has the fixed mechanism, the outside swing installation of centrifuge body has the damping mechanism, the fixed mechanism includes the mechanism board, the inside rotation of mechanism board is connected with a plurality of even distribution's fixed group. A mesenchymal stem cell separates with centrifugal device, by rotating gear ring, cooperation second gear, make multiple rotating disc synchronous rotation, and then make the clamping rod mutual approach and carry out the clamping of test tube to the test tube in the inside middle part of fixed disc, simultaneously to the test tube multidirectional force clamping, make the test tube vertical in fixed disc, and then make the fixation of test tube more stable.

The invention discloses a thalassemia treatment method based on a stem cell technology and application. The thalassemia treatment method comprises the following steps: collecting a peripheral blood sample of a patient, and carrying out stem cell isolated culture; constructing a nucleic acid aptamer by combining a vascular cell adhesion molecule-1 and a stromal cell-derived factor-1 in a bone marrow microenvironment; connecting a nucleic acid aptamer, a cell adhesion promoting peptide and a stem cell growth factor, and constructing a homing guiding reagent; preparing nano liposome and nano polymer particles; the nucleic acid aptamer is connected to the surfaces of the nano-liposome and the nano-polymer particles, and is grafted with a temperature-responsive material to obtain the nano-carrier with a responsive release function; stem cell growth factors and platelet-derived growth factors are mixed with gelatin microspheres to form a slow release system; the cultured stem cells are mixed with a homing guide reagent, a nano-carrier and a slow release system, and are transplanted into the body of a patient through intramedullary injection. The stem cell homing efficiency can be improved, and the treatment effect on thalassemia is improved.

The invention provides a construction method of a thyroid organoid derived from human induced pluripotent stem cells, and belongs to the technical field of organoid culture. The construction method of the thyroid organ comprises the following steps: (1) separating human induced pluripotent stem cells into single cells, and transferring the single cells to a 2D matrigel working solution for incubation; (2) carrying out endoderm induced culture on the cells incubated in the step (1); (3) carrying out preintestinal endoderm induced culture on the cells obtained in the step (2); (4) carrying out thyroid gland directional induction culture on the cells obtained in the step (3); (5) carrying out thyroid maturation induction culture on the cells obtained in the step (4) to obtain thyroid organs; naI with the final concentration of 0.8-1.2 [mu] M is added during thyroid maturation induction culture. The thyroid organoid constructed by the method is high in maturity, can simulate the state of the in-vivo organoid more truly, and has good capability of secreting thyroid-related hormones at the same time.

The utility model relates to the technical field of dental pulp stem cell separation, and discloses a dental pulp stem cell separation and extraction device which comprises a device shell, a driving shaft is installed in the device shell, and a stepping motor fixedly connected with the device shell is fixed to the lower end of the driving shaft. A balance mechanism used for balancing the device is arranged on the outer surface of the driving shaft, a fixing mechanism used for fixing materials is arranged at the upper end of the driving shaft, and a protection mechanism used for protection is arranged on one side of the device shell. The balance mechanism comprises an adjusting piece. Through the arrangement of the balance mechanism, the lead screw can be driven to rotate through the adjusting motor arranged in the adjusting piece, the horizontal position of the movable block can be adjusted under the cooperation of the guide rod, then the position of the balancing weight is adjusted, the centrifugal force generated when the driving shaft rotates can be stabilized, and then the shaking situation during centrifugation is reduced; and the stability of the device is improved.

The invention discloses a stem cell separation device, and belongs to the technical field of stem cell separation.The stem cell separation device comprises a base, a supporting cylinder is fixedly mounted at the top end of the base, a centrifugal cylinder is rotatably mounted in the supporting cylinder, a backflow mechanism is arranged on the outer side of the centrifugal cylinder, and the backflow mechanism comprises a first pump body mounted on the outer side of the supporting cylinder; three annular pipes are arranged between the supporting cylinder and the centrifugal cylinder and communicate with the centrifugal cylinder, the input end of the first pump body communicates with the two annular pipes arranged at the bottom end through discharging pipes, and the output end of the first pump body communicates with the annular pipe at the top end through a feeding pipe. The tissue fluid in the two annular pipes at the bottom end is absorbed through the first pump body and discharged through the annular pipe at the top end, so that a sample continuously and circularly flows in the centrifugal process, different components in the sample have more opportunities to participate in the layering process, the situation that part of stem cells are missed due to insufficient participation is avoided, and the stem cell separation accuracy is remarkably improved.

The utility model is applicable to the technical field of homogenizers, and provides a stem cell separating and extracting solution device which comprises a bottom plate, a frame is fixedly connected to the upper surface of the bottom plate, a driving assembly is arranged on the upper surface of the bottom plate, a vibration generating assembly is arranged on an output shaft of the driving assembly, a placing frame is arranged on the vibration generating assembly, and the placing frame is connected with the driving assembly. A plurality of elastic protruding balls abutting against the surface of the vibration generating assembly are arranged at the bottom of the containing frame. According to the stem cell separation extracting solution device, after a buckle is disassembled and a threaded rod is twisted, a connecting block can be driven to move, in the moving process of the connecting block, the connecting block can be driven to be far away from a fixed arc plate, locking of a placing frame is relieved, then the placing frame can be disassembled and replaced, EP pipes of different specifications can be conveniently borne and used, and the practicability is high. And a handle is arranged on the surface of the abutting shaft, and a worker can conveniently pull the abutting shaft to stretch out and draw back, so that the abutting shaft is separated from the interior of the positioning opening, and the disassembly convenience is improved.

The utility model relates to the technical field of regenerative medicine and cell therapy, in particular to a device for mechanically extracting stem cells, which comprises a hollow tube for storing tissues and a shearing plate provided with shearing holes, the shearing plate is arranged in the hollow tube and divides the hollow tube into a first chamber and a second chamber which are independent from each other, and the first chamber and the second chamber are communicated with each other. The first cavity and the second cavity are communicated through shearing holes in the shearing plate, a pushing mechanism used for organizing circulation between the first cavity and the second cavity is further arranged in the hollow pipe, two material pipes are arranged on the hollow pipe, the two material pipes are communicated with the first cavity and the second cavity respectively, and valves are arranged on the material pipes. The device for mechanically extracting the stem cells realizes homogenization treatment of tissues and efficient extraction of the stem cells through physical shearing, extrusion and bidirectional mechanical action, completely avoids time consumption of a traditional enzyme digestion method, reduces dependence on collagenase or other chemical reagents, and improves the extraction efficiency of the stem cells. The method is suitable for stem cell separation of tissues such as umbilical cord and fat with low damage and without allogenic material residue.

The invention belongs to the technical field of in-vitro culture of germline stem cells, and particularly relates to a separation and in-vitro culture method of spermatogonial stem cells of grass carp. The method comprises the following steps: by taking male grass carps as donors, anesthetizing, killing, cleaning, disinfecting, dissecting and separating gonad tissues, crushing into fragments by adopting a mode of combining magnetic bead crushing with shearing, digesting with trypsin, filtering and centrifuging to obtain high-purity spermatogonial stem cells, and performing in-vitro culture on the spermatogonial stem cells by adopting a culture solution. By optimizing the age of the male grass carp, an enzyme digestion process and an in-vitro culture system and adopting a one-step enzyme digestion method, the digestion time is shortened, the operation process is simplified, and the industrial problems that the spermatogonial stem cells of the grass carp are low in separation efficiency and culture is easy to differentiate are solved. According to the invention, a separation, purification and primary culture technical system of the spermatogonial stem cells of the grass carp is systematically established for the first time, and important cell materials and technical supports are provided for breeding technologies such as transplantation of germline stem cells of the grass carp, gene editing and the like.

This disclosure pertains to a non-living biological product. Particularly, exosomes derived from stem cells can help prevent or reduce scar tissue growth in cardiovascular system and restore heart function. According to certain embodiments, a fluid-induced, pathological shear stress mechanical stimulation process of stem cells is used to augmented quantity and quality of exosomes produced from stem cells. These exosomes serve as a therapeutic agent for preventing or reducing scar tissue growth in cardiovascular system. Therefore, compositions comprising the exosomes derived from stem cells and methods of preventing and / or treating scar tissue growth in cardiovascular system by administering the exosomes isolated from stem cells are also provided.

This invention relates to the field of stem cell isolation, and discloses a dental pulp stem cell isolation reactor and a method for efficient exosome extraction. The reactor includes a controller, a protective shell and a motor mounted on the inner wall of the controller, a rotating frame fixedly connected to the output end of the motor, a test tube slidably connected to the bottom end of the placement frame, a fixing component on the inner wall of the placement frame, toothed rings fixedly connected to both sides of the placement frame, a movable frame threadedly connected to the outer wall of the threaded rod, a rack fixedly connected to the bottom end of the movable frame, a synchronous belt meshing with the toothed end of a first synchronous wheel, and a second synchronous wheel fixedly connected to one end of the rotating wheel. By placing a test tube containing dental pulp stem cells into the placement frame and fixing it, rotating the rotating wheel causes the placement frame to rotate within the rotating frame via a transmission component to adjust the angle of the test tube. After covering with a movable cap, the controller controls the motor to drive the rotating frame to rotate, thereby changing the centrifugal force on the stem cells.

The utility model discloses a stem cell separation and detection device, which relates to the technical field of stem cell separation and detection devices and comprises a base plate, a centrifugal machine, a flow cytometer, a mounting rack and a mounting rod, the centrifugal machine and the flow cytometer are both fixedly mounted at the upper end of the base plate, and a centrifugal cylinder is arranged on the inner side of the upper end of the centrifugal machine. A supporting rod is fixedly mounted at the upper end of the centrifugal machine, a hook is arranged at the upper end of the supporting rod, and the mounting rod is arranged on the front face of the supporting rod and arranged on the back face of the supporting rod. By the adoption of the structure, after the installation rod and the installation frame are connected at the position of the supporting rod, the clamping block is pushed to slide through rotation of the first hand-screwing bolt and threaded telescopic rotation of the first hand-screwing bolt, and due to the fact that the opposite faces of the clamping block and the installation rod are provided with the V-shaped grooves, the clamping block can be rapidly installed and fixed on the supporting rod conveniently; and the operation can be carried out without a tool, so that the operation is simple and convenient.