44 results about "Fungal contamination" patented technology

An environmentally safe fungicide and its method of use are disclosed. The inventive environmentally safe fungicide comprises an effective concentration of an alkyl ester of lactic acid. Ethyl lactate is a preferred alkyl ester of lactic acid. Reduction in fungal contamination is achieved by applying the fungicide of the invention to a substrate to be decontaminated.

Isolated nucleic acids, kits and methods are disclosed for detecting, quantifying and / or identifying a fungal species in a sample or for diagnosing jejunal hemorrhage syndrome in a subject. The methods generally include amplifying a fungal nucleic acid sequence present in the sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 or SEQ. ID NO 16; and observing or measuring the amplified nucleic acid sequence. The fungal species can thereby be detected, quantified, and / or identified. Fungal contamination also can be detected or measured in a biological or feed or food sample.

The invention discloses a method for tissue culture and rapid propagation of Huzhou lily. The method comprises the following steps of: putting cleaned Huzhou lily scales into thiophanate methyl of which the concentration is 200-300mg / L, oscillating for 0.3 to 1 hour, soaking in 70-75% alcohol for 20 to 30 seconds, soaking in corrosive sublimate of which the mass volume concentration is 0.1-0.2% for 15 to 25 minutes, and rinsing out the scales; inoculating the rinsed-out scales to an induced medium to perform induced culture; after induced spouting, migrating sprouts into an enrichment medium to perform enrichment culture so as to obtain cluster buds; and inoculating the cluster buds to a strong-ball rooting medium to obtain aseptic seedlings. By adopting the method, the problem that explants of the Huzhou lily are easily subjected to fungal contamination and bacteria contamination during primary culture is solved, the contamination rate is controlled to be below 25%, and the death rate is low; and moreover, a wholes set of complete processes of primary culture, secondary culture, strong-ball culture and rooting culture is established and has high induction rate and multiplication multiple.

Isolated nucleic acids, kits and methods are disclosed for detecting, quantifying and / or identifying a fungal species in a sample or for diagnosing jejunal hemorrhage syndrome in a subject. The methods generally include amplifying a fungal nucleic acid sequence present in the sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 or SEQ. ID NO 16; and observing or measuring the amplified nucleic acid sequence. The fungal species can thereby be detected, quantified, and / or identified. Fungal contamination also can be detected or measured in a biological or feed or food sample.

The invention discloses a method for preserving pickled vegetable. The method is characterized by comprising the following steps of: peeling external withered, yellow and old leaves of Chinese cabbages which are not rotten; respectively peeling internal leaves, and performing washing, airing, draining and air-drying; putting the leaves of the Chinese cabbages in a preserving barrel layer by layer; sprinkling an appropriate amount of condiments such as salt, chicken powder, white spirit, cooking wine and cane sugar on each layer of Chinese cabbage; performing compaction, covering the barrel, and preserving for 20 to 25 days to obtain the delicious pickled vegetable. In a preserving process, water does not need to be added, and a small amount of water overflowing from the Chinese cabbages in the preserving process after cell death is needed, so that the taste of the Chinese cabbages cannot decline; in the initial stage of preserving, the cooking wine and the white spirit are added, so that the probability of fungal contamination can be reduced to a certain extent.

The present invention relates to a device for detecting a fungal contamination in an internal environment, to the use thereof and also to a method for detecting a fungal contamination in an internal environment using such a device.

A top surface of an elevating stand of a pet feeding system defines bowl receptacles into which respective food and water bowls may be removably installed. A first zone of the top surface of the stand includes an upwardly convex ring that laterally surrounds a respective bowl receptacle. A second zone of the top surface of the stand laterally surrounds the first zone, except as interrupted by a finger notch. Each bowl has a peripheral lip with a lower surface formed as a downwardly concave ring that is spaced over the upwardly convex ring of the stand. An edge of the bowl rests on the second zone at a location below the upper limit of the convex ring, thereby supporting the entire weight of the bowl and its contents on the stand. A base of the stand cooperates with upstanding ribs in an elastomeric mat to prevent lateral movement of the stand and to prevent rotation of the stand around the stand center. Compositions and shapes of the stand, bowls and mat are chosen to make them easy to clean and to reduce bacterial and fungal contamination.

A method for detecting a fungal contamination of an indoor environment by calculation of a chemical index of the fungal contamination. A sample of volatile organic compounds (VOCs) is withdrawn in indoor surroundings, and the presence or absence of certain predetermined VOCs resulting from fungal metabolism is detected.

Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

The invention relates to an LAMP (loop-mediated isothermal amplification) universal primer for detecting aspergillus toxin-producing fungi and a kit containing the primer. The LAMP primer includes inner primers FIP and BIP, outer primers F3 and B3 and loop primers LF and LB; the kit consists of the LAMP primer, Bst DNA polymerase, a reaction buffer containing dNTPs and de-ionized water. According to the LAMP primer disclosed by the invention, by applying the loop-mediated isothermal amplification technology to the rapid detection of the aspergillus toxin-producing fungi, the aspergillus toxin-producing fungi can be rapidly, accurately and conveniently detected; therefore, the LAMP primer is of great significance to the prevention and detection of contamination caused by the aspergillus toxin-producing fungi. The detection method is strong in specificity and relatively high in sensitivity, short in reaction time and strong in operability, a detection lower limit can reach 1*10<-12>g / [mu]L or below and the detection method can be achieved without an expensive instrument; in addition, the detection of a finished product is convenient and the detection can be achieved by virtue of a naked eye observation method; therefore, the detection method is quite high in practical value.

Provided is a culture medium for microorganisms present in contaminated working fluids such as coolants. More particularly, said culture medium is particularly suitable for supporting growth of microorganisms colonizing metalworking fluids and allows for specific detection of both bacterial microorganisms and fungal microorganisms the latter depending on the added selective agents which can be antibiotics for the detection of fungal contamination or fungicides for the detection of bacterial contamination. Furthermore, devices and kits comprising the culture medium of the present invention are described as well as a method of detecting microbial contamination of metalworking fluids.

The present invention relates to a device for detecting a fungal contamination in an internal environment, to the use thereof and also to a method for detecting a fungal contamination in an internal environment using such a device.

Contacting fresh fruits and fresh vegetables with an aqueous solution containing an effective amount of a neutralized silicate reduces bacterial and or fungal contamination of the fresh fruits and fresh vegetable product or retards bacterial and or fungal growth on the fresh fruits and fresh vegetable product or both without any loss to the organoleptic properties or shelf life of the product.

The invention discloses a lithium-based anti-bacteria metal surface treatment agent which comprises the following components in parts by weight: 15-20 parts of polyvinyl alcohol, 0.4-0.5 part of tea saponin, 3-4 parts of lithium lubricating grease, 0.4-1 part of ferrocenecarboxylic acid, 0.1-0.2 part of hexahydrophthalic anhydride, 0.1-0.12 part of DBNPA, 0.5-1 part of potassium sorbate, 0.1-0.2 part of hexamethyl phosphoramide, 1-2 parts of sodium bisulfite, 6-8 parts of sericin, 0.08-0.1 part of boric acid, 10-20 parts of methylbenzotriazole and 180-200 parts of deionized water. According to the invention, water-soluble polymer polyvinyl alcohol and sericin are mixed, and through modification of boric acid, tensile strength and heat stability of a blended membrane are improved, the membrane adsorbed on the surface of the metal is more uniform and stable since corrosion inhibition alkali liquor using methylbenzotriazole as a main material is added, the metal material is protected from being corroded by harmful media in atmosphere and water, a better corrosion inhibition effect is achieved, and bacterial and fungal contamination can be effectively resisted sine the added DBNPA, potassium sorbate and other various metal surface treatment agent additives are compatible.

The invention provides a simple method simple method for artificial feeding of chinese chive maggots, and belongs to the field of insect feeding. The invention uses ginger to replace the traditional artificial feeding, Chinese chives, green Chinese onions, potatoes, garlic and so on. The invention, as compared with a conventional method for feeding chinese chive maggots, has a higher survival rate, pupation rate, emergence rate and fecundity, and saves the cost and labor. In particular, the invention can avoid massive death of the chinese chive maggots or influence on the growth and development thereof caused by suppuration or rot of the above feed due to bacterial or fungal contamination, and can lay a solid foundation for a large number of tests on the chinese chive maggots and provide effective protection.

The invention discloses a cyrtomium fortunei spore reproduction method, and belongs to the technical field of the plant reproduction. The cyrtomium fortunei spore reproduction method comprises the following steps: cyrtomium fortunei spores are collected from September to December, and impurity removal is carried out on the collected cyrtomium fortunei spores; the cyrtomium fortunei spores are disinfected by using hydrogen peroxide, disinfection is carried out on a ground substance by using a high-temperature autoclaved sterilization method, the spores are spread to a nutrition pot with a spore germination ground substance, and the nutrition pot is placed in a greenhouse; the top of the nutrition pot is covered with preservative film used for sealing so that water evaporation and fungal contamination can be prevented, and a temperature ranging from 25 to 30 DED C and an illumination intensity ranging from 2500 to 4500 lx are maintained; when two leaves grow out of a sporophyte, the sporophyte is transplanted to a seedling cultivation ground substance and is stored in a shading tent, a temperature ranges from 28 to 33 DEG C is maintained, a relative humidity ranges from 80 to 90 percent is maintained, and water is sprayed for every six to eight hours. According to the cyrtomium fortunei spore reproduction method, the operation is simple, an over 85 percent transplanting survival rate of spores seedlings is reached, the output is high with little input, the health and strong seedlings is provided for a cyrtomium fortunei artificial cultivation, and the value of practicality and popularization is high.

The invention discloses a method for detecting fungal pollution producing aflatoxin by combining GC-IMS and fluorescence spectrum, which comprises the following steps: performing sample treatment, GC-IMS detection, fluorescence spectrum detection, GC-IMS spectrogram characteristic region selection, fluorescence spectrum characteristic region selection, data characteristic extraction and fusion of GC-IMS and fluorescence spectrum detection, principal component analysis on the extracted feature data, then extractiion of principal components of first ten dimensions of PCA of the two data sources as novel feature vectors, and direct combination of the novel feature vectors together to form fusion data; and performing orthogonal partial least squares discrimination on the fused data, inputting the data after feature fusion into an orthogonal partial least squares discrimination model, taking a test set sample data feature value of a fused signal as input, and obtaining a final classification model through an orthogonal partial least squares discrimination algorithm, so that the method is applied to detection of an unknown sample. The method is simple and easy to operate, operation cost is reduced, and the method has good classification performance and high popularization and utilization value.

The invention discloses application of bacillus licheniformis to the promotion of rooting or growth of semen trigonellae tissue culture seedlings. The application comprises the bacterium activation of a raw material entrocoor dinatibiogen and inoculation to successive semen trigonellae seedlings in form of bacillus licheniformis liquid, a large number of separation, purification and strain identification steps are eliminated, the addition of any exogenous hormones is avoided, the cost is low, the operation is easy, safe and nontoxic, and remarkable effects are achieved; the rooting rate of the inoculated semen trigonellae tissue culture seedlings is remarkably increased, indexes such as plant height and leaf area are also improved, the survival rate after fungal contamination is greatly increased, and the rooting quality of the semen trigonellae tissue culture seedlings is remarkably improved.

The invention discloses a method for treating contamination of suspension culture cells. The specific steps of the treatment method for suspension culture cells after contamination are as follows: S1, identification of pollution types, S2, treatment of fungal pollution, S3 treatment of mycoplasma pollution, S4 Bacterial contamination treatment. The method first distinguishes different pollution categories through different identification methods, and then treats different pollution sources separately. Compared with the traditional treatment method after cell pollution occurs, the present invention is simple and easy to implement, and has better treatment effect.

The invention relates to a method for processing fungal contamination in a cordyceps militaris culture process. According to the method, the culture medium surface generating fungal contamination in the culture process is covered by river sand absorbed with fungicide. The processing method provided by the invention has the advantages that the operation is simple and convenient, raw material resources are wide, the cost is low, and the fungal contamination in the cordyceps militaris culture process can be conveniently and fast processed. The cordyceps militaris fruit bodies obtained after the processing have high yield and high quality, so the processing method provided by the invention has wide application prospects.

A microbe-resistant coating composition or paint including an antimicrobial agent is described. The antimicrobial agent is an inorganic bismuth-containing compound, and may be used in conjunction with other bismuth-containing compounds or other biocidal agents or methods. A method of treating an electrodeposition system for bacterial and / or fungal contamination is also described by adding an antimicrobial agent to at least a portion of the electrodeposition system.

The present invention provides a stable, low free formaldehyde, synergistic antimicrobial composition, and a method of inhibiting the growth of or reducing microorganisms by applying the composition. Preferably, the constituents are dimethylol dimethylhydantoin, monomethylol dimethylhydantoin, dimethyl hydantoin, and dehydroacetic acid or its salts thereof. The composition has a free formaldehyde content of less than 0.2% and is beneficial for controlling microbiological and fungal contamination in personal care products, household products and industrial products and systems.

In order to achieve the purposes, the invention provides a preparation method of a bamboo powder feed based on microbial fermentation. The preparation method comprises the following steps of carryingout selecting and trimming; cleaning and soaking; drying; crushing, sieving and weighing for later use; mixing and compounding microorganisms to obtain fermented materials; bagging; shading fermentation; inspecting; labeling; warehousing, that is, performing vacuum packaging in different bags, taking moso bamboo rods and bamboo leaves as raw materials and crushing into 40 to 100 meshes of bamboo powder, wherein under the condition that the bamboo hole complete state is maintained in a non-wall-breaking preservation mode, fermentation of composite microorganisms is carried out to accelerate growth of probiotic flora, while fermentation is carried out by vacuum anaerobic fermentation in an assisting mode, and stable fermentation is avoided while a situation of fungus pollution is avoided; and by means of the beneficial bacteria with activity, the feed is still kept in a uniform and loose state after being formed, the feed is used for feeding, the intestinal health is promoted, the disease-prevention and harm immunity is improved, the livestock is promoted to effectively absorb feed nutrition, and the purpose of producing high-quality moso bamboo fermented feed is achieved.

The invention discloses a chrysanthemum tissue culture method. The present invention comprises preparation of a bacteriostatic culture medium for a combination of various bacteriostatic agents, and inoculates the Penicillium grisea viridifera that exists widely in nature and is easy to extract, so that the Penicillium grisea viridiens is in a latent state under the action of the bacteriostatic agents, and It does not cause fungal contamination, and the growth and development status of the chrysanthemum tissue culture seedlings cultivated in this medium and the performance after hardening are all normal, and has no effect on the yield and quality of chrysanthemum seedlings; the chrysanthemum group in the antibacterial medium The cultured seedlings were inoculated into ordinary culture medium, and there was no inhibitory effect of bacteriostatic agents. The tissue cultured seedlings broke out fungal contamination in a large area and the contamination rate reached 100%. Through this method, while ensuring the production efficiency and quality of chrysanthemum tissue culture seedlings, it can effectively protect the intellectual property varieties of chrysanthemums. Only units with the right to grant varieties can obtain healthy tissue culture seedlings to prevent the illegal outflow of fine varieties.

The invention discloses a method for tissue culture and rapid propagation of Huzhou lily. The method comprises the following steps of: putting cleaned Huzhou lily scales into thiophanate methyl of which the concentration is 200-300mg / L, oscillating for 0.3 to 1 hour, soaking in 70-75% alcohol for 20 to 30 seconds, soaking in corrosive sublimate of which the mass volume concentration is 0.1-0.2% for 15 to 25 minutes, and rinsing out the scales; inoculating the rinsed-out scales to an induced medium to perform induced culture; after induced spouting, migrating sprouts into an enrichment medium to perform enrichment culture so as to obtain cluster buds; and inoculating the cluster buds to a strong-ball rooting medium to obtain aseptic seedlings. By adopting the method, the problem that explants of the Huzhou lily are easily subjected to fungal contamination and bacteria contamination during primary culture is solved, the contamination rate is controlled to be below 25%, and the death rate is low; and moreover, a wholes set of complete processes of primary culture, secondary culture, strong-ball culture and rooting culture is established and has high induction rate and multiplication multiple.

The invention relates to a high-efficiency bacillus (Bacillus sp.) capable of degrading straws and preventing and treating fungal diseases of plants. The bacillus disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.22829. The bacillus provided by the invention has the function of efficiently degrading straws, can obviously inhibit the growth of plant pathogenic fungi, has the advantages of no environmental pollution and ecological safety, and can be widely applied to the fields of plant pathogenic fungi, plant pathogenic fungi and the like. Particularly, the composition is used for preventing and treating various alternaria fungi such as pear black spot and potato early blight as well as cirrhospora macromaculata fungi, and can be used for preventing and treating cirrhospora macromaculata fungi. Besides, the strain is added into the feed for fermentation treatment, so that the formed biological feed can effectively prevent pathogenic fungus pollution of the feed, and the resistance replacement effect is achieved.