40 results about "Exogenous hormones" patented technology

The invention discloses a fructus alpiniae oxyphyllae culture medium and application thereof. Belongs to the technical field of tissue culture. The culture medium comprises a callus induction and proliferation culture medium, a cluster bud differentiation and proliferation culture medium and a rooting culture medium. Each culture medium is based on the cheap and easily available MS or 1 / 2 MS culture medium, appropriate exogenous hormones or raw materials are added, each culture medium is scientific and reasonable in formula and can be respectively used for each stage of tissue culture of the alpinia oxyphylla, and the whole culture process is high in propagation speed, short in period and high in survival rate; the excellent hereditary character of the alpinia oxyphylla variety can be well maintained. The culture medium is simple in formula and low in cost, and has good popularization and application prospects.

The invention provides a cultivation method for increasing the kernel rate of dried small-fruit camellia oleifera, and belongs to the technical field of crop cultivation. The invention provides a cultivation method for improving the dry kernel rate of small-fruit camellia oleifera, which comprises the following steps: carrying out variety configuration by taking a clone camellia oleifera variety as a main cultivated variety and taking a common camellia oleifera variety with high dry kernel rate as a matched cultivated variety; meanwhile, after variety configuration is completed, a fertilization scheme is optimized, and exogenous hormones are sprayed in the critical growth period of the small-fruit camellia oleifera. Through scientific variety configuration, fertilization scheme optimization, exogenous hormone spraying and other cultivation technologies, the dry kernel rate of camellia oleifera is effectively increased. By means of the cultivation method, the dry kernel rate of the small-fruit camellia oleifera can be remarkably increased, the camellia oleifera yield and quality are improved, meanwhile, the method has the advantages of being easy and convenient to operate, low in cost and the like, is easy to apply and popularize in camellia oleifera production areas, and has important significance in promoting sustainable and healthy development of the camellia oleifera industry.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The invention discloses a method for regulating and controlling the quality of picked honey pomelo fruits. The meizhou honey pomelo fruits are soaked by using 1.0 g / L MT and 0.5 g / L NAA exogenous hormone solution and then are preserved after being picked, so that the meizhou honey pomelo treated by the exogenous hormone keeps relatively high quality in the aspects of freshness, smell, moisture content, flavor and mouth feel; the preservative has good effects in the aspects of weight loss, juice sac granulation, respiration, degradation of vitamin C, acid return and the like of meizhou honey pomelo fruits, and has important significance in providing a new thought for post-harvest preservation of the meizhou honey pomelo.

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating perimenopausal syndrome, which is composed of podophyllum hexandrum, lagotis, honeysuckle fruit, bamboo shavings, pilose antler and sea-buckthorn. The composition takes podophyllum hexandrum as a monarch drug, sea-buckthorn and honeysuckle fruit as ministerial drugs to enhance the effects of promoting blood circulation and regulating menstruation, cornu cervi pantotrichum for replenishing essence and blood, warming yang and dispelling cold, and bamboo shavings and lagotis herb as adjuvant drugs for cooling blood and clearing heat, so that a three-dimensional system of promoting blood circulation, tonifying and clearing heat is formed. It is verified that the composition can relieve uterine atrophy, improve organic decline of a reproductive system, reduce whole blood viscosity and abnormally-increased leukocyte number, inhibit inflammation, accurately adjust serum E2, LH, PROG and AMH levels so as to remodel hormone balance and avoid side effects of exogenous hormones. The composition is based on the advantages of Tibetan medicines and the multi-target effect of natural medicinal materials, conforms to the modern research and development trend and is high in clinical transformation value.

The invention discloses a sterile production method of a fresh dendrobium officinale protocorm product, which comprises the following steps: taking dendrobium officinale seeds from capsules of dendrobium officinale, repeatedly washing the capsules with tap water until the capsules are free of foam, sterilizing, cutting the capsules open, and drying to obtain the dendrobium officinale protocorm product. Uniformly spreading the seeds on an M2 solid culture medium, keeping the illumination condition of 500Lx and the temperature of 25 + / -1 DEG C, and waiting for the seedlings to grow for 60-90 days; wherein the M2 solid culture medium comprises 1 / 2 MS macroelements without exogenous hormones, 35% of banana juice, 35% of composite carbon source and 2% of agar powder. The method has the beneficial effects that a technical route map for mass production by infinitely stacking sterilization and ventilation culture of the small glass containers of 10 liters and getting rid of the constraint of a shaking table is provided. The technical key point is to screen out that the growth rate which can reach 8.4 mg / g.d without depending on plant growth hormone (which is a rigid biological index).

The invention discloses a method and device for cultivating garlic seedlings by using aerial bulbs, and belongs to the technical field of agricultural bioengineering.The method comprises the steps that after dormancy of the aerial bulbs is broken through an exogenous hormone solution, the aerial bulbs are cleaned and disinfected through sterile water, a sodium hypochlorite solution and sterile water, then the aerial bulbs are cultivated in a culture medium solution till sprouting, and the garlic seedlings are obtained; according to the cultivation method, the exogenous hormone solution circularly flows to soak the aerial bulbs, real-time regulation and control can be realized by monitoring the concentration of the exogenous hormone solution in the circulating liquid box, and the exogenous hormone solution in the cultivation box is always kept in a set concentration range by regulating and controlling the concentration of the solution in the liquid box; the isoconcentration soaking can better break the dormancy of the aerial bulbs and improve the inductivity of the aerial bulbs, and the circulating device also reduces the waste of an exogenous hormone solution, can realize reutilization, and saves the cost of large-batch cultivation.

The invention is suitable for the technical field of camellia oil application, and provides application of camellia oil in prevention and treatment of ovarian function decline. The invention reveals that the camellia oil has good prevention and improvement effects on the ovarian function decline for the first time. A series of rigorous scientific experiments prove that the camellia oil can effectively improve weight loss and reproductive hormone disorder caused by mouse ovarian function decline induced by cyclophosphamide, repair ovarian tissue damage and inhibit ovarian granular cell apoptosis, so that normal development of ovaries is promoted, the ovarian index is increased, and the effect of preventing and treating ovarian diseases is achieved. The number of atresia follicles is reduced, the pregnancy rate and the litter size are increased, and the occurrence of ovarian function decline is effectively prevented and improved. The discovery provides a scientific basis for preventing and improving ovarian function decline by using the camellia oil, and avoids tissue hyperplasia and canceration risks caused by strong stimulation of an endocrine system, reproductive organs and mammary gland by exogenous hormones in a hormone therapy.

The invention discloses a dendrobium officinale protocorm differentiation inhibition and rapid propagation method. The method comprises the four steps of seed disinfection treatment, protocorm induction, protocorm domestication and protocorm propagation. According to the method, the protocorms are effectively generated by utilizing seeds, a liquid domestication mode is innovatively adopted, 1 mu M of salicylic acid is added into a liquid culture medium, differentiation of the protocorms is remarkably inhibited, the survival rate of the protocorms when the protocorms are transferred from the liquid culture medium to a solid culture medium is increased, and a large number of protocorm products are rapidly obtained through a hormone-free culture scheme; therefore, an efficient and sustainable production process is realized. In the whole cultivation process, no exogenous hormone or chemical synthetic substance is used, the method completely depends on a biological mechanism generated by natural induction and organic matter induction, and the naturalness and safety of the product are guaranteed from the source. According to the method, the production efficiency of the protocorm is improved, the production cycle of dendrobium related products is shortened, and the production cost of the protocorm is reduced.

The invention provides an ictalurus punctatus dnmt1 gene knockout RNAi target sequence, a sex regulation and control method and application, and belongs to the technical field of sex regulation and control. An RNAi sequence is designed based on the dnmt1 gene, and gonad differentiation intervention independent of exogenous hormones is realized in the species by inhibiting expression of the dnmt1 gene in the channel catfish. Experimental results show that dnmt1 expression down-regulation can effectively induce partial sex reversal of genetic female individuals, typical testis-like structures appear in ovarian tissues of the female individuals, it is proved that dnmt1 plays a key role in maintaining female gonad development stability, and it is hopeful that dnmt1 regulation is combined with a genetic screening or gene editing technology, so that the development stability of the female gonad is improved. A stable and efficient all-male channel catfish breeding system is established, and a new path is developed for all-male offspring seed production.

The present invention discloses a method for simultaneously detecting multiple precocious puberty biomarkers; the method comprises the following steps: 1) taking a urine sample and adding an internal standard solution; 2) enzymolysis: adding a glucuronidase solution to the sample solution of step 1) for enzymolysis; 3) the samples after enzymolysis are respectively loaded on an HLB solid phase extraction plate and a MAX solid phase extraction plate, and after washing with a rinsing liquid to remove interfering substances, the samples are eluted with an eluent, and the eluents of the HLB solid phase extraction plate and the MAX solid phase extraction plate are combined, and UPLC-MS detection is performed to obtain the contents of multiple precocious puberty biomarkers; the multiple precocious puberty biomarkers include exogenous hormone compounds, plasticizers, bisphenol A compounds, and endogenous hormone compounds; the detection types of the multiple precocious puberty biomarkers are 102 or more. The present invention is easy to operate and greatly improves the detection speed, throughput, and types and quantity of precocious puberty biomarkers covered.

The invention discloses pseudomonas fluorescens SMP5 which is derived from rhizosphere soil of salvia miltiorrhiza. The invention further provides application of the pseudomonas fluorescens SMP5 in growth promotion and quality improvement of the artemisia annua, the pseudomonas fluorescens SMP5 obtained through screening and identification can achieve efficient colonization on the surfaces of leaves of the artemisia annua, and plant growth and quality improvement are remarkably promoted in a foliage spraying mode. Compared with an existing traditional method depending on chemical fertilizer or exogenous hormone, the strain can effectively promote leaf yield and biomass accumulation (the yield and the biomass are increased by 41.04% and 44.92% respectively), the secretory glandular hair density is remarkably increased by 4.4 times, and more structural bases are provided for artemisinin synthesis. According to the method, a complete regulation and control chain of beneficial bacterium-leaf surface-glandular hair-artemisinin synthesis is established, double improvement of quality and yield is achieved, and the method has the advantages of being environmentally friendly, safe and capable of being popularized.

The invention discloses a material-saving-free cultivation method and application of eucalyptus tomentosa seedlings. The cultivation method comprises the step of spraying an exogenous hormone aqueous solution in stages in the 3-8-month-old period of the eucalyptus tomentosa seedlings: (1) at the first stage, spraying a gibberellin aqueous solution; (2) a second stage: spraying a brassinolide aqueous solution; and (3) a third stage: spraying a mixed aqueous solution containing brassinolide and abscisic acid. The material-saving-free cultivation method for the eucalyptus tomentosa nursery stock is used for material-saving-free eucalyptus tomentosa cultivation, synchronous regulation and control of trunk height promotion and lateral branch inhibition of the eucalyptus tomentosa nursery stock can be achieved, the trunk cleaning height and the trunk cleaning rate are remarkably improved, and early-stage material-saving-free formation of the eucalyptus tomentosa is promoted.

The application discloses a Dendrobium candidum protocorm differentiation inhibition and rapid propagation method, which comprises four steps of seed disinfection treatment, protocorm induction, protocorm domestication and protocorm proliferation. μ M salicylic acid, significantly inhibits protocorm differentiation and improves the survival rate of protocorm when it is transferred from a liquid culture medium to a solid culture medium, and a hormone-free culture scheme is used to quickly obtain a large amount of protocorm product, thereby realizing an efficient and sustainable production process. In the whole cultivation process, no exogenous hormones or chemical synthetic substances are used, and the biological mechanism generated by natural induction and organic induction is completely relied on, so that the natural and safety of the product are guaranteed from the source. The application improves the production efficiency of the protocorm, shortens the production cycle of the Dendrobium-related product and reduces the production cost of the protocorm.

The present application relates to the technical field of plant genetic engineering, and particularly relates to application of LkHY5 gene in regulation of somatic embryogenesis of hybrid larch. The present application shows that overexpression of LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. Overexpression of LkHY5 gene significantly increases expression levels of LkGBSS1-3 and LkTAN1-1. Overexpression of LkHY5 gene significantly increases contents of starch, maltose, glucose and soluble sugar in callus. Overexpression of LkHY5 gene significantly increases microtubule stability in callus.

The invention relates to the technical field of seed priming, and discloses a sweet menu embryo seed priming device and an exogenous hormone priming method.A workbench is provided with a priming pipe used for containing an exogenous hormone priming solution and seeds, the rear portion of the workbench is provided with a support, and the support is connected with a temperature control device through a hanging assembly; the bottom of the temperature control device extends into the initiation pipe and is used for regulating and controlling the temperature of the initiation solution; valve assemblies are fixed to the bottoms of the triggering pipes. When the air introduction pipeline system introduces air into the initiation pipe, the rotating assembly is driven to rotate, so that an initiation solution and seeds are spirally stirred, the temperature of the initiation solution is uniformly regulated and controlled by the temperature control device, and the air or oxygen is uniformly mixed with the initiation solution; after the control valve on the discharge pipe is opened, the initiation solution and the seeds completely flow out through the discharge pipe under the stirring of the rotation assembly on the initiation solution and the seeds, so that the seeds are thoroughly discharged without manual cleaning.

The invention discloses a method for promoting effective coloring of Nouronia queen grapes. According to the method, a five-dimensional superposition regulation and control system with girdling induction, shading and bag removal, water and nutrient control, exogenous hormone supplement, spectral energy enhancement and canopy microclimate optimization is constructed, grape fruit development and pigment metabolism processes are accurately intervened in stages, and anthocyanin synthesis and accumulation are systematically promoted. According to the method, the fruit coloring rate and the coloring uniformity are improved, the effective coloring window period is prolonged, and the commodity fruit rate and the fruit sugar-acid ratio are remarkably increased. Field tests prove that the fruit coloring rate can be increased to 90% or above, the commodity fruit rate can be increased to 80% or above, and the output value per mu is remarkably increased. The invention provides a systematic solution for solving the technical problems of poor coloring, unstable quality and the like of the Naironia queen grapes, and has good popularization and application values.

The invention discloses an FvePREs transcription factor and application thereof in promoting strawberry fruit expansion, compared with a traditional expansion agent, the scheme has the advantages that the safety is improved, and the existing expansion agent may cause malformed fruits and hollow fruits and has chemical residue risk. The FvePREs transcription factor regulates and controls fruit development through a molecular level, exogenous hormones are not needed, and chemical pollution and potential food safety hazards are avoided; the scheme is more accurate in action mechanism, the existing biological agent (such as a formula containing alginic acid and brassinolide) needs to depend on external application, and the effect is influenced by environmental factors. The gene technology directly acts on a plant endogenous regulation and control system, and the synergistic effect of fruit expansion and quality optimization is achieved by activating or inhibiting specific gene expression; the scheme has sustainability, the overexpression FvePREs transcription factor can change the genetic characteristics of strawberries, so that the strawberries can keep a good expansion effect in the long-term planting process, dependence on external chemical substances is reduced, and sustainable agricultural development is facilitated.

The invention discloses an exogenous hormone formula for promoting cutting rooting of pinus massoniana, and relates to the technical field of plant cutting, and the technical scheme is as follows: the exogenous hormone formula comprises the following components in percentage by mass: 40% of IBA, 40% of NAA and 20% of D-iso-VC; the preparation method comprises the following steps: weighing 0.4 g of IBA, 0.4 g of NAA and 0.2 g of D-iso-VC, dissolving the IBA, the NAA and the D-iso-VC in 50ml of pure ethanol, and adding distilled water to a constant volume of 500ml after 20 minutes of thorough dissolution so as to prepare mother liquor; the mother liquor needs to be diluted when being used, and a prepared solution is obtained by diluting the mother liquor and distilled water according to the proportion of 1: 4; the using process of the prepared solution comprises the steps that after a masson pine semi-lignified scion is subjected to elution treatment, the lower end of the masson pine semi-lignified scion is soaked in the prepared solution for 15 min, and then cuttage is conducted. The method can efficiently solve the problem of low cutting rooting rate of the pinus massoniana, promotes vegetative propagation of the pinus massoniana, and effectively keeps and utilizes excellent characters of parents.

The invention relates to the technical field of plant genetic engineering, in particular to application of an LkHY5 gene in regulation and control of hybrid larch somatic embryogenesis. Results of the invention show that overexpression of the LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. The expression levels of LkGBSS1-3 and LkTAN1-1 are remarkably improved by overexpression of the LkHY5 gene. The overexpression of the LkHY5 gene significantly improves the contents of starch, maltose, glucose and soluble sugar in the callus. The overexpression of the LkHY5 gene significantly improves the stability of the callus microtubules.

The invention relates to a method for promoting yield increase and quality improvement of peony seeds, which comprises the following steps: after pollination of peony is completed, spraying an exogenous hormone gibberellin GA3 solution or brassinolide BR solution to fruit pods of peony plants, and spraying to the fruit pods to enable liquid beads to drip. By means of the method, generation and growth of peony seeds are promoted, the number of normal peony seeds is greatly increased, the number of abortive seeds is reduced, and after gibberellin GA3 or brassinolide BR is sprayed for treatment, the number of the normal seeds is increased by 50% or above and can reach 2-3 times; the number of abortive seeds is reduced by more than 20%; the size of the peony seeds is increased, the length and width of the seeds are obviously increased, the problem of environmental pollution easily caused by massive fertilization is effectively avoided, the production cost is reduced, and the method is easy to operate and popularize and has important significance for promoting the industrialization development of the peony.

The present invention relates to a pre-flowering exogenous hormone spraying device for corn, wherein a liquid storage chamber is provided inside the backpack spraying box, an exogenous hormone box is installed at the lower end of the backpack spraying box, an exogenous hormone storage chamber is provided inside the exogenous hormone box, and the lower end surfaces of the liquid storage chamber and the exogenous hormone storage chamber are both arranged as arc-shaped surfaces gathered toward the middle, a liquid outflow pipe is installed at the lower end of the liquid storage chamber, an exogenous hormone suction pipe is arranged in the middle of the exogenous hormone storage chamber, the exogenous hormone suction pipe and the liquid outflow pipe are both connected to a mixing pipe, the mixing pipe is connected to a mixing mechanism, and the mixing mechanism is connected to an exogenous hormone outlet pipe. Before use, liquid and high-concentration, paste-like / ointment-like exogenous hormone dispersion are temporarily diluted to the actual concentration used, and are homogenized by a mixing mechanism with a spiral flow channel. The present invention solves the problem that the exogenous hormones in the current exogenous hormone spraying are not prepared and used immediately, and are easy to deteriorate, resulting in reduced or lost activity.

The invention relates to the technical field of aquaculture, in particular to a rana nigromaculata early breeding and froglet breeding method and system. On the basis of temperature control and water body stimulation of'fullness, drying and refullness', awakening and delivery are promoted under the condition of no exogenous hormone; a stepped terrain is constructed in the same temperature control greenhouse, the water level and the water-land ratio are adjusted in a linkage mode according to a population metamorphosis rate threshold value, and continuous operation of incubation, cultivation, metamorphosis and food domestication is achieved in cooperation with a water inlet and outlet net bag with the replaceable mesh number, an anti-escape fence and moving / film covering food tables on the two sides. Compared with a conventional pond dividing mode, breeding can be advanced by more than or equal to 2 months, and froglets appear on the market to July; the survival rate of incubation and metamorphosis is improved, drowning is remarkably reduced, the success rate of domestication and the specification uniformity are improved, meanwhile, carrying stress is reduced, and the unit fry cost is reduced.

The invention provides a shaping and pruning method for preventing and treating citrus fruit cracking, and belongs to the field of citrus cultivation. According to the method, 5-8 spindling branches with the length of 60-80 cm at the top of a tree crown are reserved through high-top pruning, annual high-quality bearing branches with the diameter larger than or equal to 0.4 cm are selected for fruit setting, and the fruit cracking rate is remarkably reduced in combination with an annual updating mechanism of the spindling branches. Exogenous hormone application or special fertilizer and water management is not needed, operation is easy and convenient, cost is low, applicability is wide, and good application and popularization prospects are achieved.

The present invention provides a method for quickly growing seedlings from sweet cherry seeds, which relates to the field of growing seedlings from sweet cherry seeds. The method includes separating the pulp from the fruit pit of the sweet cherry fruit, putting the fruit pit into a fine mesh bag, washing it, soaking it in a disinfectant solution, rinsing it, and drying the fruit pit after draining the water; putting the stored fruit pit into a container, soaking it, floating out the floating seeds, and reserving the fruit pit at the bottom for later use; knocking the reserved fruit pit to break the pit shell, taking out the kernel, and using the kernel as the sweet cherry seed; after rinsing the sweet cherry seed, putting it into an exogenous hormone solution, and standing it still in the dark at room temperature, disinfecting the treated sweet cherry seed, placing the disinfected sweet cherry seed on a culture dish lined with wet filter paper, and putting the culture dish into an artificial climate chamber; when 90% of the sweet cherry seeds turn green, sowing them in a nutrient bowl filled with vermiculite, watering, and spraying a nutrient solution on the emerged seedlings; hardening off the seedlings, and when the seedlings grow to 10 cm in height, transplanting them to a greenhouse. The present invention can effectively and quickly break the dormancy of the seeds and enable the sweet cherry seeds to germinate quickly.

The invention belongs to the technical field of plant cell engineering, and particularly relates to a culture method of a hormone autotrophic ginseng cell line and the ginseng cell line. The culture method comprises the following steps: peeling, cleaning and disinfecting a ginseng embryo, slicing, inoculating to an MS solid culture medium, and inducing to obtain an embryogenic cell line; transferring to an MS liquid culture medium for suspension culture to obtain a stable suspension cell line; carrying out nursing culture after treatment with a composite mutagenic agent; and transferring the growing cell cluster to a hormone-free MS solid culture medium for multiple subculture, and screening out a cell line independent of exogenous auxin. The cell line can proliferate without exogenous hormones, the residual risk is eliminated, the cost is reduced, and the cell line is suitable for the fields of food and medicine and has important application value.

The invention provides a gene influencing morphogenesis of an asparagus lettuce plant as well as application and a regulation and control mechanism, and belongs to the technical field of gene engineering. The invention provides a gene LsGA20ox1 influencing morphogenesis of an asparagus lettuce plant, after overexpression of the gene LsGA20ox1, the stem length of the plant is significantly increased, the lignin content is increased, a hollow phenomenon occurs, the chlorophyll content in the stem is significantly increased, but the chlorophyll content of leaves is significantly reduced; the CRISPR knockout mutant shows a dwarfing phenotype, and the content of chlorophyll in leaves is increased; meanwhile, the content of endogenous gibberellin GA19 in overexpressed plants is remarkably reduced, and the content of GA20 is remarkably increased; in a gene knockout plant, the content of endogenous gibberellin GA19 is remarkably increased, and the content of GA20 is greatly reduced. The invention provides a mechanism for regulating and controlling the jointing and hollowing of the asparagus lettuce by a gibberellin metabolic pathway for the first time, and provides a theoretical support for establishing a genetic improvement strategy independent of exogenous hormone treatment.

This invention relates to the field of animal reproductive regulation technology, and discloses a twinning regulator for sika deer males that synchronize estrus and its application method. The regulator is a homogeneous and transparent sol system prepared from trehalose, pituitary follicle-stimulating hormone (FSH), medium- and low molecular weight sodium hyaluronate, low molecular weight dextran sulfate, zinc gluconate, and physiological saline. This invention utilizes sodium hyaluronate of a specific molecular weight and dextran sulfate to construct a polysaccharide sol network, combined with the cross-linking effect of zinc gluconate and the physicochemical stabilizing effect of trehalose, to encapsulate and slowly release FSH. The application method includes implanting and removing a vaginal silicone plug, combined with the injection of pregnant mare serum gonadotropin (PMSG) and the regulator, followed by natural mating. This invention prolongs the half-life of exogenous hormones in the animal, avoids drastic fluctuations in blood drug concentration, and improves the stability of twinning rates in sika deer.

The application discloses a kind of to cultivate the method and its device of air-borne bulb to garlic seedling, belong to agricultural bioengineering technical field, after breaking dormancy with exogenous hormone solution, air-borne bulb is washed and disinfected by sterile water, sodium hypochlorite solution, sterile water, then is cultivated in culture medium solution to sprout after, obtain garlic seedling;The cultivation method of the application can break the dormancy of air-borne bulb by making exogenous hormone solution circulate and flow soak air-borne bulb, can be monitored in circulating liquid box exogenous hormone solution concentration to real-time control, by controlling the concentration of solution in liquid tank, the exogenous hormone solution in culture box always maintains the concentration range set, soak at equal concentration can better break the dormancy of air-borne bulb, improve the induction rate of air-borne bulb, and the waste of exogenous hormone solution is reduced by circulating device, can be used again, save the cost of large-scale cultivation.