27 results about "Exogenous hormones" patented technology

The invention provides a cultivation method for increasing the kernel rate of dried small-fruit camellia oleifera, and belongs to the technical field of crop cultivation. The invention provides a cultivation method for improving the dry kernel rate of small-fruit camellia oleifera, which comprises the following steps: carrying out variety configuration by taking a clone camellia oleifera variety as a main cultivated variety and taking a common camellia oleifera variety with high dry kernel rate as a matched cultivated variety; meanwhile, after variety configuration is completed, a fertilization scheme is optimized, and exogenous hormones are sprayed in the critical growth period of the small-fruit camellia oleifera. Through scientific variety configuration, fertilization scheme optimization, exogenous hormone spraying and other cultivation technologies, the dry kernel rate of camellia oleifera is effectively increased. By means of the cultivation method, the dry kernel rate of the small-fruit camellia oleifera can be remarkably increased, the camellia oleifera yield and quality are improved, meanwhile, the method has the advantages of being easy and convenient to operate, low in cost and the like, is easy to apply and popularize in camellia oleifera production areas, and has important significance in promoting sustainable and healthy development of the camellia oleifera industry.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The invention discloses a method for regulating and controlling the quality of picked honey pomelo fruits. The meizhou honey pomelo fruits are soaked by using 1.0 g / L MT and 0.5 g / L NAA exogenous hormone solution and then are preserved after being picked, so that the meizhou honey pomelo treated by the exogenous hormone keeps relatively high quality in the aspects of freshness, smell, moisture content, flavor and mouth feel; the preservative has good effects in the aspects of weight loss, juice sac granulation, respiration, degradation of vitamin C, acid return and the like of meizhou honey pomelo fruits, and has important significance in providing a new thought for post-harvest preservation of the meizhou honey pomelo.

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating perimenopausal syndrome, which is composed of podophyllum hexandrum, lagotis, honeysuckle fruit, bamboo shavings, pilose antler and sea-buckthorn. The composition takes podophyllum hexandrum as a monarch drug, sea-buckthorn and honeysuckle fruit as ministerial drugs to enhance the effects of promoting blood circulation and regulating menstruation, cornu cervi pantotrichum for replenishing essence and blood, warming yang and dispelling cold, and bamboo shavings and lagotis herb as adjuvant drugs for cooling blood and clearing heat, so that a three-dimensional system of promoting blood circulation, tonifying and clearing heat is formed. It is verified that the composition can relieve uterine atrophy, improve organic decline of a reproductive system, reduce whole blood viscosity and abnormally-increased leukocyte number, inhibit inflammation, accurately adjust serum E2, LH, PROG and AMH levels so as to remodel hormone balance and avoid side effects of exogenous hormones. The composition is based on the advantages of Tibetan medicines and the multi-target effect of natural medicinal materials, conforms to the modern research and development trend and is high in clinical transformation value.

The invention discloses a sterile production method of a fresh dendrobium officinale protocorm product, which comprises the following steps: taking dendrobium officinale seeds from capsules of dendrobium officinale, repeatedly washing the capsules with tap water until the capsules are free of foam, sterilizing, cutting the capsules open, and drying to obtain the dendrobium officinale protocorm product. Uniformly spreading the seeds on an M2 solid culture medium, keeping the illumination condition of 500Lx and the temperature of 25 + / -1 DEG C, and waiting for the seedlings to grow for 60-90 days; wherein the M2 solid culture medium comprises 1 / 2 MS macroelements without exogenous hormones, 35% of banana juice, 35% of composite carbon source and 2% of agar powder. The method has the beneficial effects that a technical route map for mass production by infinitely stacking sterilization and ventilation culture of the small glass containers of 10 liters and getting rid of the constraint of a shaking table is provided. The technical key point is to screen out that the growth rate which can reach 8.4 mg / g.d without depending on plant growth hormone (which is a rigid biological index).

The invention provides an ictalurus punctatus dnmt1 gene knockout RNAi target sequence, a sex regulation and control method and application, and belongs to the technical field of sex regulation and control. An RNAi sequence is designed based on the dnmt1 gene, and gonad differentiation intervention independent of exogenous hormones is realized in the species by inhibiting expression of the dnmt1 gene in the channel catfish. Experimental results show that dnmt1 expression down-regulation can effectively induce partial sex reversal of genetic female individuals, typical testis-like structures appear in ovarian tissues of the female individuals, it is proved that dnmt1 plays a key role in maintaining female gonad development stability, and it is hopeful that dnmt1 regulation is combined with a genetic screening or gene editing technology, so that the development stability of the female gonad is improved. A stable and efficient all-male channel catfish breeding system is established, and a new path is developed for all-male offspring seed production.

The invention discloses pseudomonas fluorescens SMP5 which is derived from rhizosphere soil of salvia miltiorrhiza. The invention further provides application of the pseudomonas fluorescens SMP5 in growth promotion and quality improvement of the artemisia annua, the pseudomonas fluorescens SMP5 obtained through screening and identification can achieve efficient colonization on the surfaces of leaves of the artemisia annua, and plant growth and quality improvement are remarkably promoted in a foliage spraying mode. Compared with an existing traditional method depending on chemical fertilizer or exogenous hormone, the strain can effectively promote leaf yield and biomass accumulation (the yield and the biomass are increased by 41.04% and 44.92% respectively), the secretory glandular hair density is remarkably increased by 4.4 times, and more structural bases are provided for artemisinin synthesis. According to the method, a complete regulation and control chain of beneficial bacterium-leaf surface-glandular hair-artemisinin synthesis is established, double improvement of quality and yield is achieved, and the method has the advantages of being environmentally friendly, safe and capable of being popularized.

The invention discloses a material-saving-free cultivation method and application of eucalyptus tomentosa seedlings. The cultivation method comprises the step of spraying an exogenous hormone aqueous solution in stages in the 3-8-month-old period of the eucalyptus tomentosa seedlings: (1) at the first stage, spraying a gibberellin aqueous solution; (2) a second stage: spraying a brassinolide aqueous solution; and (3) a third stage: spraying a mixed aqueous solution containing brassinolide and abscisic acid. The material-saving-free cultivation method for the eucalyptus tomentosa nursery stock is used for material-saving-free eucalyptus tomentosa cultivation, synchronous regulation and control of trunk height promotion and lateral branch inhibition of the eucalyptus tomentosa nursery stock can be achieved, the trunk cleaning height and the trunk cleaning rate are remarkably improved, and early-stage material-saving-free formation of the eucalyptus tomentosa is promoted.

The application discloses a Dendrobium candidum protocorm differentiation inhibition and rapid propagation method, which comprises four steps of seed disinfection treatment, protocorm induction, protocorm domestication and protocorm proliferation. μ M salicylic acid, significantly inhibits protocorm differentiation and improves the survival rate of protocorm when it is transferred from a liquid culture medium to a solid culture medium, and a hormone-free culture scheme is used to quickly obtain a large amount of protocorm product, thereby realizing an efficient and sustainable production process. In the whole cultivation process, no exogenous hormones or chemical synthetic substances are used, and the biological mechanism generated by natural induction and organic induction is completely relied on, so that the natural and safety of the product are guaranteed from the source. The application improves the production efficiency of the protocorm, shortens the production cycle of the Dendrobium-related product and reduces the production cost of the protocorm.

The present application relates to the technical field of plant genetic engineering, and particularly relates to application of LkHY5 gene in regulation of somatic embryogenesis of hybrid larch. The present application shows that overexpression of LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. Overexpression of LkHY5 gene significantly increases expression levels of LkGBSS1-3 and LkTAN1-1. Overexpression of LkHY5 gene significantly increases contents of starch, maltose, glucose and soluble sugar in callus. Overexpression of LkHY5 gene significantly increases microtubule stability in callus.

The invention discloses an FvePREs transcription factor and application thereof in promoting strawberry fruit expansion, compared with a traditional expansion agent, the scheme has the advantages that the safety is improved, and the existing expansion agent may cause malformed fruits and hollow fruits and has chemical residue risk. The FvePREs transcription factor regulates and controls fruit development through a molecular level, exogenous hormones are not needed, and chemical pollution and potential food safety hazards are avoided; the scheme is more accurate in action mechanism, the existing biological agent (such as a formula containing alginic acid and brassinolide) needs to depend on external application, and the effect is influenced by environmental factors. The gene technology directly acts on a plant endogenous regulation and control system, and the synergistic effect of fruit expansion and quality optimization is achieved by activating or inhibiting specific gene expression; the scheme has sustainability, the overexpression FvePREs transcription factor can change the genetic characteristics of strawberries, so that the strawberries can keep a good expansion effect in the long-term planting process, dependence on external chemical substances is reduced, and sustainable agricultural development is facilitated.

The invention relates to the technical field of plant genetic engineering, in particular to application of an LkHY5 gene in regulation and control of hybrid larch somatic embryogenesis. Results of the invention show that overexpression of the LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. The expression levels of LkGBSS1-3 and LkTAN1-1 are remarkably improved by overexpression of the LkHY5 gene. The overexpression of the LkHY5 gene significantly improves the contents of starch, maltose, glucose and soluble sugar in the callus. The overexpression of the LkHY5 gene significantly improves the stability of the callus microtubules.

The invention relates to the technical field of aquaculture, in particular to a rana nigromaculata early breeding and froglet breeding method and system. On the basis of temperature control and water body stimulation of'fullness, drying and refullness', awakening and delivery are promoted under the condition of no exogenous hormone; a stepped terrain is constructed in the same temperature control greenhouse, the water level and the water-land ratio are adjusted in a linkage mode according to a population metamorphosis rate threshold value, and continuous operation of incubation, cultivation, metamorphosis and food domestication is achieved in cooperation with a water inlet and outlet net bag with the replaceable mesh number, an anti-escape fence and moving / film covering food tables on the two sides. Compared with a conventional pond dividing mode, breeding can be advanced by more than or equal to 2 months, and froglets appear on the market to July; the survival rate of incubation and metamorphosis is improved, drowning is remarkably reduced, the success rate of domestication and the specification uniformity are improved, meanwhile, carrying stress is reduced, and the unit fry cost is reduced.

The invention provides a shaping and pruning method for preventing and treating citrus fruit cracking, and belongs to the field of citrus cultivation. According to the method, 5-8 spindling branches with the length of 60-80 cm at the top of a tree crown are reserved through high-top pruning, annual high-quality bearing branches with the diameter larger than or equal to 0.4 cm are selected for fruit setting, and the fruit cracking rate is remarkably reduced in combination with an annual updating mechanism of the spindling branches. Exogenous hormone application or special fertilizer and water management is not needed, operation is easy and convenient, cost is low, applicability is wide, and good application and popularization prospects are achieved.

The invention belongs to the technical field of plant cell engineering, and particularly relates to a culture method of a hormone autotrophic ginseng cell line and the ginseng cell line. The culture method comprises the following steps: peeling, cleaning and disinfecting a ginseng embryo, slicing, inoculating to an MS solid culture medium, and inducing to obtain an embryogenic cell line; transferring to an MS liquid culture medium for suspension culture to obtain a stable suspension cell line; carrying out nursing culture after treatment with a composite mutagenic agent; and transferring the growing cell cluster to a hormone-free MS solid culture medium for multiple subculture, and screening out a cell line independent of exogenous auxin. The cell line can proliferate without exogenous hormones, the residual risk is eliminated, the cost is reduced, and the cell line is suitable for the fields of food and medicine and has important application value.

This invention relates to the field of animal reproductive regulation technology, and discloses a twinning regulator for sika deer males that synchronize estrus and its application method. The regulator is a homogeneous and transparent sol system prepared from trehalose, pituitary follicle-stimulating hormone (FSH), medium- and low molecular weight sodium hyaluronate, low molecular weight dextran sulfate, zinc gluconate, and physiological saline. This invention utilizes sodium hyaluronate of a specific molecular weight and dextran sulfate to construct a polysaccharide sol network, combined with the cross-linking effect of zinc gluconate and the physicochemical stabilizing effect of trehalose, to encapsulate and slowly release FSH. The application method includes implanting and removing a vaginal silicone plug, combined with the injection of pregnant mare serum gonadotropin (PMSG) and the regulator, followed by natural mating. This invention prolongs the half-life of exogenous hormones in the animal, avoids drastic fluctuations in blood drug concentration, and improves the stability of twinning rates in sika deer.

The application discloses a kind of to cultivate the method and its device of air-borne bulb to garlic seedling, belong to agricultural bioengineering technical field, after breaking dormancy with exogenous hormone solution, air-borne bulb is washed and disinfected by sterile water, sodium hypochlorite solution, sterile water, then is cultivated in culture medium solution to sprout after, obtain garlic seedling;The cultivation method of the application can break the dormancy of air-borne bulb by making exogenous hormone solution circulate and flow soak air-borne bulb, can be monitored in circulating liquid box exogenous hormone solution concentration to real-time control, by controlling the concentration of solution in liquid tank, the exogenous hormone solution in culture box always maintains the concentration range set, soak at equal concentration can better break the dormancy of air-borne bulb, improve the induction rate of air-borne bulb, and the waste of exogenous hormone solution is reduced by circulating device, can be used again, save the cost of large-scale cultivation.

The invention discloses a method for regulating and controlling tobacco leaf surface bacterial colonies through exogenous hormones and application, and relates to the technical field of tobacco agriculture. Comprising the following steps: 1) preparing a working solution; (2) adding a nonionic surfactant; and 3) field spraying. The invention aims to fill the technical blank of field application of plant hormones through a systematic, normative and scientific method, and provides a reproducible and generalizable solution for quality improvement and disease prevention and control of crops such as tobacco and the like.

The invention discloses a tea tree tissue culture-free regeneration method based on leaf cutting, which comprises the following steps: firstly, selecting mature and healthy leaves of a tea tree variety, and reserving complete petioles; then soaking the petiole base part of the leaf with a first exogenous hormone solution, and then cutting the leaf into a substrate; illumination, temperature and air humidity conditions are set for culture, a second exogenous hormone solution is sprayed to the leaves, and the leaves are sequentially induced to grow calluses, adventitious roots and adventitious buds. By adopting a tissue culture-free mode, through specific hormone treatment and environment regulation and control, the tea tree leaves form calluses, adventitious roots and adventitious buds at the petiole bases, and finally complete plants are formed, so that the regeneration efficiency of tea tree leaf cuttage can be remarkably improved, meanwhile, rooting and germination are realized, the complete plants are formed, and the survival rate of the tea tree leaves is increased. The method has a wide application prospect and an important economic value.

The invention relates to the technical field of plant tissue culture and asexual propagation, and particularly discloses a tuber section propagation method for nephrolepis auriculata, which comprises the steps of endophyte selective enrichment culture, section preparation, synergistic induction culture, acclimatization and transplantation and the like. Wherein the formula composition of the rooting and germination culture medium is associated with the carbon source selection of the endophyte growth-promoting culture medium; according to the method, a staged plant-microorganism collaborative culture system based on carbon source adaptation is established, so that the biological function of tuber endophyte flora is effectively utilized, and the breeding efficiency and the seedling quality are remarkably improved; according to the method, the biological stability of a culture system is enhanced while the exogenous hormone dependence is reduced, an innovative and efficient solution is provided for solving the problems of high pollution rate, insufficient seedling robustness, large transplanting survival rate fluctuation and the like in traditional tuber propagation, and the method is suitable for large-scale and standardized production.

This invention discloses a method for the artificial breeding and selection of small plateau native ornamental fish, belonging to the field of aquaculture technology. The method includes: S1 broodstock domestication, S2 hormone-free spawning induction, S3 fertilized egg hatching, S4 graded initial domestication, and S5 juvenile fish selection. In S2, the broodstock are placed in a spawning induction device, where visual mirror stimulation is generated through a reflecting mirror. Combined with the synergistic effect of gradually increasing light intensity from 8 hours to 12 hours and water temperature from 16°C to 22°C, and the weak electric field generated by the bottom microelectrode array (intensity below the fish's perception threshold, waveform is sine or square wave), natural spawning is induced without the addition of exogenous hormones, achieving a spawning rate of over 87.5%. In S4, the fry are fed graded micro-particle feed in three stages, with particle sizes of 80-120μm, 120-160μm, and 160-200μm, respectively. The fry feeding rate reaches over 87%, and the survival rate at 35 days of age reaches over 78%. This invention enables the artificial breeding of native ornamental fish in the plateau with zero hormones, and is simple to operate with a high survival rate.

The present application relates to the technical field of livestock breeding regulation, and discloses a natural inducer for promoting the estrus synchronization of cows, a preparation method and application, comprising the following steps: first, collecting natural musk of adult male forest musk deer and uniformly mixing the same to obtain musk raw materials; then, adding the musk raw materials into a solvent to obtain a solid-liquid mixture; then, placing the solid-liquid mixture in an ultrasonic device for ultrasonic extraction, so that the active ingredients in the musk raw materials are dissolved; finally, removing the precipitate and collecting the supernatant to obtain the natural inducer. The chemical signal is transmitted to the hypothalamus through the olfactory pathway, avoiding the pain stress and the risk of inflammation at the injection site caused by muscle injection; at the same time, the reproductive cycle is regulated by activating the neuroendocrine system of the cow itself, rather than directly supplementing exogenous hormones, thereby fundamentally eliminating the hidden danger of exogenous hormone residues in cow's milk or beef, meeting the requirements of modern livestock farming for green and healthy breeding.

This invention discloses a method for off-season hydroponic cultivation of Aralia elata buds based on plant hormone regulation, belonging to the field of hydroponic culture technology. This off-season hydroponic method optimizes the concentration ratio of indolebutyric acid (IBA), gibberellin (GA3), and salicylic acid through orthogonal experiments, combined with standardized branch pretreatment, hormone immersion, and ozone water culture management, to achieve efficient off-season cultivation of Aralia elata buds. This off-season hydroponic culture uses exogenous hormone regulation, optimized for the off-season dormancy characteristics of Aralia elata and the physiological needs of bud growth. It effectively activates the physiological metabolic pathways within the branches, quickly breaking the natural dormancy state, while providing suitable hormone guidance for bud differentiation and growth. This results in uniform bud sprouting and robust growth, solving the problems of late bud break from dormancy, slow bud growth, and large differences in growth vigor among different plants, laying a solid foundation for efficient off-season hydroponic production.

The present application relates to the field of plant biotechnology, in particular to a method for rescuing seedlings from Malus robusta and Malus baccata far-off hybridization zygotes and application, and the method is summarized as follows: a method for rescuing seedlings from Malus robusta and Malus baccata far-off hybridization zygotes is provided.The present application carries out interspecific far-off hybridization by taking Malus robusta as female parent and Malus baccata as male parent.According to the degeneration of far-off hybridization zygotes, the zygotes are rescued.The present application determines the suitable sampling period for rescuing zygotes of hybrid combinations, the concentration and ratio of exogenous hormones required for zygote germination, proliferation and expansion, rooting culture, and the suitable hardening and transplanting method.The zygote rescue system for interspecific hybridization of Malus robusta and Malus baccata established by the present application has a zygote germination rate of 74.3%, a proliferation coefficient of 5.1, a rooting rate of 95.5% in rooting culture, and a hardening and transplanting survival rate of 96.4%.

The invention relates to a method for judging the quality of ova of Japanese eels based on external forms, which comprises the following steps: (1) obtaining the ova of the Japanese eels after artificial ripening and induced spawning of the Japanese eels by utilizing exogenous hormones; (2) observing the external form of the ovum through a microscope and measuring the diameter of the ovum; meanwhile, the quality of the ovum is judged by observing the color and sedimentation time of the ovum in seawater with naked eyes and observing the water absorption expansion effect of the ovum and the position of a cell nucleus in an oocyte through a microscope. According to the method, the quality of the ova of the Japanese eels is judged through naked eye observation and microscope observation, the method is convenient, rapid and effective, and scientific support is provided for development of research on the artificial breeding technology of the Japanese eels.

The invention discloses a somatic embryo induction method and an in-vitro regeneration method of camellia plant leaves, and belongs to the technical field of plant tissue culture. The method comprises the steps of initial explant preparation, sterile leaf acquisition, leaf organ proliferation and exogenous hormone endogenesis, leaf initial dedifferentiation, dedifferentiation homeostasis, somatic embryo induction and proliferation, somatic embryo maturation, germination, secondary seedling strengthening and the like, and is combined with comparison and screening experiments to determine an optimal culture medium formula; a stable and efficient somatic embryo induction and regeneration system is established, the somatic embryo induction rate and regeneration rate can be improved, the breeding process is accelerated, the resource waste in the production process is reduced, and a support is provided for genetic improvement and industrial seedling culture of camellia plants.