19 results about "Dwarfing" patented technology

The invention belongs to the technical field of plant bioengineering, and discloses a KASP primer group for detecting wheat plant height and yield synergistically improved QTL locus QPH-6A and application, the primer group is designed for SNP loci at the physical positions 11, 164 and 425 bp of wheat 6A chromosomes, and the primer group comprises two specific forward primers for different alleles and a universal reverse primer. By utilizing the primer group and the detection method provided by the invention, whether the wheat material carries the QPH-6A excellent allele or not can be quickly and accurately identified. The excellent allelic gene has a unique'dwarfing without yield reduction 'genetic effect, and the thousand seed weight, the ear length and the ear seed number can be remarkably increased while the plant height is reduced to a suitable lodging-resistant range (75-85 cm). According to the invention, the problems of too short plant, reduced biomass, premature senility and the like caused by the traditional dwarf gene in arid ecological regions such as Xinjiang are solved, and an efficient breeding tool is provided for cultivating a new high-yield ideal plant type wheat variety suitable for an arid environment.

The application relates to the technical field of plant breeding, and particularly relates to application of an SLR1 protein mutant or a coding gene thereof in regulating population yield and harvest index of plants. The SLR1 protein mutant is obtained by mutating at least one of tyrosines at positions 94 and 580 into alanine on the basis of a wild-type SLR1 protein. The application comprises the following steps: improving the function of the SLR1 protein mutant or the expression level of a coding gene thereof for the plants, and improving the plant type, lodging resistance, yield or harvest index of the plants. A SLR1 protein (a member of the DELLA protein family) mutant is obtained through the research of the application, and when the SLR1 protein in the plants is mutated, a semi-dwarf plant type can be obtained, the ammonium nitrogen utilization rate, the nitrate nitrogen utilization rate and the biomass are not greatly affected, the yield and the harvest index are significantly improved, and the application is superior to an sd1 green revolution variety, which has important application value in the field of plant breeding.

This invention discloses the application of the SbGA20ox3 gene in regulating sorghum variety improvement and breeding, belonging to the field of biotechnology. This invention involves targeted editing of the SbGA20ox3 gene in sorghum, resulting in a significant dwarfing phenotype with a dwarfing efficiency of 37.01%–50.67%. Simultaneously, it reduces the number of internodes, shortens internode length, reduces the length of the main spikelet stalk, and increases stem diameter, while increasing cellulose and lignin content, significantly enhancing lodging resistance. Furthermore, it simultaneously optimizes the tannin and amylopectin content of sorghum grains, improving quality traits. The gene-edited sorghum obtained by this invention does not contain transgenic elements, and other agronomic traits are unaffected. It can target and improve sorghum plant architecture, stress resistance, and quality, providing key gene targets and practical technical systems for molecular breeding of dwarfed, lodging-resistant, high-quality, and high-yield sorghum, and has significant agricultural application value.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PcWVD2 gene in enhancing the dwarfing ability of pear plants. The CDS (Coding Sequence) of the PcWVD2 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the PcWVD2 gene is as shown in SEQ ID NO. 2. The invention reveals that the PcWVD2 gene has the function of regulating and controlling the dwarf character of the pear plant, the dwarf ability of the pear can be obviously enhanced by over-expressing the gene, the dwarf ability shows that the plant height is reduced, the internode is shortened and the root length is shortened, and an important candidate gene is provided for dwarf close planting cultivation of the pear tree. According to the method for enhancing the dwarfing ability of the pear plant, the endogenous dwarfing gene PcWVD2 is directly overexpressed by the pear plant through genetic transformation, the stably inherited dwarfing character can be realized, the dependence on dwarfing rootstock can be gotten rid of, and the cultivation management cost can be reduced.

The invention discloses application of a Zm00001d033798 gene in regulation and control of the height of a corn plant, and belongs to the technical field of regulation and control of the height of the corn plant. The corn plant height key regulation gene Zm00001d033798 is cloned and functionally verified for the first time, the nucleotide sequence of the corn plant height key regulation gene Zm00001d033798 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO: 2. By inhibiting or knocking out the function of the gene, the plant height of the corn can be stably reduced by about 20-40 cm, the dwarfing effect is derived from uniform shortening between stem sections, and key yield characters such as spike length are not influenced. The invention provides application of a DNA molecule containing the gene, a recombinant vector, an expression cassette and a related gene editing tool (such as specific sgRNA) to reduction of corn plant height and cultivation of dwarf close planting resistant varieties. The gene provides a fully verified efficient target for corn plant type improvement and molecular breeding, and has important application value.

The invention relates to the technical field of plant breeding, in particular to application of an SLR1 protein mutant or a coding gene thereof in regulating and controlling the population yield and the harvest index of plants. The SLR1 protein mutant is obtained by mutating at least one of tyrosine at the 94th site and tyrosine at the 580th site into alanine on the basis of wild type SLR1 protein. The application comprises the following steps: aiming at the plant, improving the function of the SLR1 protein mutant or the expression level of the coding gene of the SLR1 protein mutant, and improving the plant type, lodging resistance, yield or harvest index of the plant. The SLR1 protein (DELLA protein family member) mutant is obtained through research, when the SLR1 protein in a plant is mutated, a semi-dwarf plant type can be obtained, meanwhile, the ammonium nitrogen utilization rate, the nitrate nitrogen utilization rate and the biomass are not greatly influenced, the yield and the harvest index are remarkably improved, and the SLR1 protein mutant is superior to an sd1 green revolution variety and has a broad application prospect. The method has important application value in the field of plant breeding.

The invention relates to the field of corn gene functions and application, and particularly discloses application of a Zm00001d016801 gene in regulating and controlling the height of a corn plant. The gene is specifically and highly expressed in corn stalk tissues, and the expression quantity of the gene is remarkably and positively correlated with the plant height. The expression or protein activity of the Zm00001d016801 gene is inhibited through technologies such as gene editing and the like, so that a corn material of which the plant height is obviously reduced and other important agronomic characters are not obviously influenced can be obtained. The key function of the Zm00001d016801 gene in corn plant height regulation and control is disclosed for the first time, a mutant of the gene shows a whole internode uniformly shortened dwarfing phenotype, the tasseling and pollen scattering period and the ear yield character are not affected, and the common problem of'one short part and both loss' in application of an existing dwarfing gene is effectively solved. The invention provides a new molecular target and an effective tool for corn plant type improvement and density-tolerant lodging-resistant breeding, and has an important breeding application value.

The invention relates to the technical field of gene engineering, in particular to application of a CmESI gene in regulating and controlling dwarfing of a muskmelon plant. According to the invention, methods of genetic analysis, mixed pool sequencing, Mutmap and Kasp genotype analysis and the like are combined to locate a gene causing extreme dwarfing of a plant, an SNP variation site of G / A existing in a first basic group of a seventh exon of a CmESI gene in a mutant esi is found out, the variation site causes CmESI to generate a variable shearing form with a retained sixth intron, and the mutation site is used for detecting the extreme dwarfing of the plant. A termination codon is formed in advance and cannot be translated into complete protein, so that the CmESI gene loses functions; the expression of CmESI in the 13C germplasm is inhibited by using a virus-induced gene silencing technology, and the plant height of a TRSV-CmESI plant is obviously lower than that of a control group plant; therefore, the muskmelon CmESI gene has certain theoretical significance and practical value in regulating and controlling the height of muskmelon plants.

The application discloses a method for cultivating transgenic plants with dwarfing, increased tillering and lightened leaf color and application thereof, and is based on preparing a plant interference vector capable of silencing or interfering with normal expression of an OsASR5 gene to regulate expression of the OsASR5 gene in the transgenic plant, so that the transgenic plant is particularly characterized by dwarfing, increased tillering and lightened leaf color, and thus the specified characteristics can be easily obtained. Moreover, the method is simple in operation, remarkable in interference effect and high in success rate, and provides favorable technical support for directional cultivation of the transgenic plant.

The invention relates to a composition with an insecticidal effect, a pesticide and application thereof, and belongs to the technical field of pest control. The composition disclosed by the invention can be used for effectively preventing and treating piercing-sucking mouthpart pests such as aleyrodid and green peach aphid. According to verification of the embodiment of the invention, in a field pesticide effect test on tomato bemisia tabaci, the control effect of the composition disclosed by the invention is as high as 93.45% after the composition is applied for 10 days; meanwhile, in the prevention and treatment of peach aphids, under the same dosage, the prevention effect after 14 days can still be maintained at a high level of 90.78%. It is fully proved that the composition is good in fast-acting property and outstanding in persistence, and recovery and rerampant of pest populations can be effectively controlled. Moreover, the composition disclosed by the invention is safe to the growth of test crops such as tomatoes and peach trees, no phytotoxicity symptoms such as dwarfing, chlorosis and malformation are observed after the composition is applied, and the composition shows good crop compatibility.

Currently, most reported rice dwarfing mutants exhibit drastic changes in plant height, severely impacting yield and thus hindering practical application in breeding. Furthermore, there is a severe lack of "fine-tuning genes" capable of slightly dwarfing plant height under the SD1 background. This application relates to a gene, SDV1, that regulates plant height. Mutants, alleles, or derivatives can be generated by adding, substituting, inserting, or deleting one or more nucleotides in the nucleotide sequences shown in SEQ ID NO.2, SEQ ID NO.3, or SEQ ID NO.4. This application clarifies the function of two dwarfing mutants in regulating rice plant height development through phenotypic analysis, genetic analysis, gene cloning, and expression analysis, providing valuable gene resources for rice lodging resistance breeding.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a... VviGAI Application of gene mutants and recombinant vectors in dwarf grape breeding. VviGAI Gene mutants can reduce the height of grapevines, the aforementioned VviGAI Gene mutants are derived from wild-type mutants. VviGAI The wild-type gene is obtained by a point mutation in which the base T at position 113 is replaced by the base A. VviGAI The nucleotide sequence of the gene is shown in SEQ ID NO.1. VviGAI The nucleotide sequence of the gene mutant is shown in SEQ ID NO.2. (Regarding grapes...) VviGAI By performing site-directed mutations in the genes, new grape germplasm with stable dwarfing traits can be obtained, providing excellent materials for grape greenhouse cultivation, potted ornamental cultivation, and intensive production.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker co-segregated with a dwarf melon plant type, a KASP molecular marker and application of the SNP molecular marker and the KASP molecular marker, and belongs to the technical field of molecular genetics. The SNP molecular marker is located at the 1145461 site nucleotide of a No.7 chromosome of a muskmelon genome, namely the 51st site of a nucleotide sequence as shown in SEQ ID No.1, and the nucleotide polymorphism is C or G. When the genotype of the SNP molecular marker is homozygous GG, the muskmelon shows a dwarf plant type; when the genotype of the SNP molecular marker is homozygous CC or heterozygous CG, the plant height of the muskmelon is normal. The KASP molecular marker is designed according to the SNP molecular marker, and comprises three primers, namely K1145461-F, K1145461C-R and K1145461G-R, gene typing of the SNP molecular marker can be quickly realized through a PCR (Polymerase Chain Reaction) reaction, plant type dwarfing variation is assisted to be introduced into a melon with normal plant height, and genetic improvement of the plant type of the melon is promoted.

The application relates to a D9 mutant protein and application thereof in regulating plant height, prolonging flowering period and maintaining grain yield, and belongs to the technical field of plant gene breeding. The D9 mutant protein is obtained by mutating glycine at the 571th position of D9 protein into valine, and the amino acid sequence is shown as SEQ ID NO. 1. The application finds that the key amino acid site G571 in the GRAS domain of the D9 protein affects the D9-GID1 interaction, predicts and verifies that the G571V mutation at the amino acid site has the functions of dwarfing the plant, moderately prolonging the flowering period and not reducing the grain yield of the plant, thereby providing a solid theoretical basis for the research and development of a dwarfing and non-reducing yield type corn variety.

The application relates to the technical field of fruit tree breeding, and discloses a hybrid breeding method for breeding cold-resistant dwarf rootstocks by using Xinjiang wild apples, which comprises the following steps: Xinjiang wild apples are used as female parents, and conventional dwarf rootstocks M9 are used as male parents to cross and extract seeds; the seeds are put into a reaction kettle, and a composite seed soaking liquid containing a citric acid-disodium hydrogen phosphate buffer solution, polyethylene glycol, dimethyl sulfoxide, tert-butyl hydrogen peroxide and gibberellin is pumped in for constant-temperature stirring reaction; then linear cooling is carried out, and citric acid aqueous solution is synchronously added dropwise to lower the pH value; after constant-temperature and constant-pH reaction, liquid is discharged, the seeds are leached, soaked and rehydrated, and finally, the seeds are placed in a dark environment for static buffering recovery and germination acceleration. The application breaks the dormancy of hybrid seeds by using chemical stress combined with cold and acid double environments, shortens the breeding period, and completes early screening of cold resistance adaptability before germination, so that the acquisition rate of cold-resistant excellent single plants is obviously improved.

The invention relates to the technical field of plant breeding, in particular to application of SIP12 protein or a coding gene thereof to regulation and control of plant height characters of plants. The SIP12 protein comprises an amino acid sequence as shown in SEQ ID NO. 1 (Sequence Identifier Number 1). The coding gene comprises a nucleotide sequence as shown in SEQ ID NO. 2 or 3. The application comprises the step of reducing the plant height of a plant by reducing the expression level of the SIP12 protein in the plant or the coding gene of the SIP12 protein. The SIP12 protein mainly related to plants is obtained through research, and the plant height can be effectively reduced by reducing the expression level of the protein. The SIP12 protein and the application thereof provided by the invention can be used for cultivating dwarf plants, and have important application value in the field of plant breeding.

The invention relates to a D9 mutant protein and application thereof in regulating plant height, prolonging flowering phase and maintaining grain yield, and belongs to the technical field of plant gene breeding, the D9 mutant protein is obtained after glycine on the 571th site of D9 protein is mutated into valine, and the amino acid sequence of the D9 mutant protein is shown as SEQ ID NO.1. The key amino acid site G571 influencing D9-GID1 interaction in the GRAS structural domain of the D9 protein is found, it is predicted and verified that the G571V mutation on the amino acid site can dwarf plants, meanwhile, the flowering phase can be moderately prolonged, the function that the grain yield of the plants is not reduced is achieved, and a solid theoretical basis is provided for research and development of dwarfing and yield-reducing-free type corn varieties.

The invention belongs to the technical field of molecular biological detection, and particularly relates to an SNP molecular marker of a sorghum plant height related gene SbDW2 and application. The SNP molecular marker is screened from the sorghum plant height related gene SbDW2, the molecular marker is located at the 549th site of the SbDW2 gene sequence, a primer combination for detecting the site is designed, and precise typing of the SbDW2 gene can be achieved in the whole growth period of sorghum. The molecular marker is directly linked with gene function variation, the detection cost is low, the efficiency is high, the breeding period can be shortened when the molecular marker is applied to sorghum breeding, the genetic basis of dwarf sorghum genes is widened, and a key basis is provided for cultivating new lodging-resistant high-yield sorghum varieties.