339 results about "Mutation breeding" patented technology

The invention belongs to the technical field of microalga biology, and particularly relates to a microalga strain with high CO2 tolerance and high fixation rate and a breeding method thereof. The microalga is named Chlorella sp.Y-1, and is collected into Common Microorganism Center of China General Microbiological Culture Collection Committee; and collection number is CGMCC No.5429. The invention has the following characteristics: the initial strain is separated from natural environment; after high-CO2-concentration enrichment culture, gene cloning, induced mutation breeding and other operations, the carbon content of the target strain is increased to 56.981%, the optimal fixation concentration is up to 20% (v/v), the fixation rate is up to 5.762g/(L.d), and the maximum tolerance concentration is 100% (v/v); and the target strain has high adaptability to a series of physical and chemical culture conditions, has high culture transfer stability, and high better overall properties than the like research findings at present. The invention aims to solve the bottleneck problem in high concentration CO2 biological fixation technology, cultures an efficient organism capable of efficiently fixing high concentration CO2, implements effective fixation of CO2 in high concentration flue gas and other complex environments, and meanwhile, generates beneficial biological substances.

The invention relates to a novel normal-pressure room-temperature plasma induced mutation breeding device. The novel normal-pressure room-temperature plasma induced mutation breeding device comprises a sample processing system, a cooling system, a control system and a detection system; the sample processing system comprises a clean working chamber without bioactive contaminants; a step motor and an object stage are arranged in the clean working chamber; the object stage is arranged on the step motor; at least one sterilizing device mounting position is reserved in the inner cavity or on the wall of the clean working chamber; the detection system comprises a gas flow controller, a temperature sensor and a position sensor; and the control system comprises an operating panel and a controller, and the controller is used for controlling the step motor to realize rising and falling or horizontal rotation of the object stage so as to automatically process a plurality of samples. The novel normal-pressure room-temperature plasma induced mutation breeding device is capable of realizing the functions of continuous automatic processing, automatic sterilization, automatic monitoring and control on the samples, and also capable of completing the plasma induced mutation breeding in the normal pressure and room temperature environment at higher efficiency.

The invention discloses an induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery, which comprises a bud starting culture medium, a test-tube seedling enrichment medium, a first somatic embryo and/or adventive bud formation culture medium, a second somatic embryo and/or adventive bud formation culture medium, a third somatic embryo and/or adventive bud formation culture medium, a first somatic embryo and/or adventive bud growth and differential medium, a second somatic embryo and/or adventive bud growth and differential medium, and a sound seedling rooting medium. By using the induced rapid propagation culture medium for the somatic embryos of the leaves in vitro of the photinia x frasery, a basal culture medium and content are improved, and the culture condition is adjusted to ensure that the photinia x frasery which is difficult to propagate propagates rapidly through the occurring pathway of the somatic embryos, the plant transplantation survival rate is over 95 percent, a seedling grows healthily, so that the problem of quality reduction of a good seed can be effectively solved, a large amount of high-quality purified and rejuvenated virus-free nursery stocks can be provided for production, and an ideal acceptor system can be provided for genetic transformation or mutation breeding seed selection of the photinia x frasery.

The invention relates to a protoplast mutation breeding method for improving the cordycepin content of cordyceps militaris. The method comprises the following steps of activated culture of bacterial strains, solid culture of mycelia, liquid mycelia culture, mycelia enzymolysis, protoplast nitrosoguanidine (NTG) mutation, protoplast regeneration culture, regenerated bacteria subculture, fermentation culture, filtering, further washing of mycelia, measurement of cordycepin content in the mycelia, preservation of the bacteria strain with high cordycepin content, measurement of the genetic stability and the like. Due to the adoption of the method, the cordycepin content of the cordyceps militaris can be increased.

The invention provides a bacteria halomonas huanghegensis BJGMM-B45, which has excellent salt tolerance. The preservation number of the halomonas huanghegensis BJGMM-B45 is CGMCC (China General Microbiological Culture Collection Center) NO.7022. The bacteria not only can be directly used as functional bacteria for improvement of saline-alkali soil, but also can be used as a gene donor of a novel transgenic organism; the organism can obtain excellent stress resistance if an excellent salt tolerance gene is transferred into the organism; in addition, the bacteria also can be used as a novel gene engineering bacteria, and can obtain more excellent properties by accepting other foreign excellent genes; furthermore, more mutant strains with excellent properties can be obtained through improvement of properties by traditional mutation breeding manner.

The invention discloses a Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and an application in the fermentation production of hyaluronic acid. The SXY36 strains of Streptococcus equi subsp. Zooepidemicus subspecies and a method for controlling HA molecular weight using the fermentation process are provided. The method has the advantages that SXY36 is subjected to irradiation mutation breeding by ultraviolet rays, microwave and gamma rays, no hemolysis exists, the HA fermentation yield is high, and the heredity is stable, in the optimal fermentation condition, the fermentation yield of shaking flask is up to 0.731 g/L, and the yield of fermentation by a fermentation cylinder can reach 4.78 g/L; a two-stage variable temperature fermentation and stable pH method is adopted, high molecular-weight HA can be prepared by fermentation, and the molecular weight can reach 1.89 MDa, which is two times the molecular weight by a conventional fermentation method; HA with the molecular weight in the range of 0.035-1.89 MDa can be produced by using a fermentation broth self-enzymatic degradation method to prepare different molecular-weight HA and by controlling the time of enzymatic hydrolysis.

The invention discloses a method for breeding microorganisms by plasma-induced mutation, which radiates microorganisms to be induced to mutate with a plasma jet flow that uses neutral active particles as acting particles to obtain mutated microorganisms. The method in which the neutral active particles in the plasma jet flow play a main induction role has the advantages of: ensuring the high activity of forward mutant strains and the high capability and genetic stability of produced target products, reducing mutagenesis period and improving mutagenesis efficiency, inducing a great variety of microorganisms such as prokaryotic microorganisms, eukaryotic microorganisms and archaebacteria in form of microbial community, bacterial suspension or spore suspension, simplifying experimental operation by avoiding culturing the processed microorganisms in the dark, having the characteristics of simple, safe and pollution-free whole operation, low equipment and experiment cost and the like and having great potential role in the field of industrial microorganism induced mutation breeding.

The invention relates to a red yeast engineering bacterium for high-yield production of astaxanthin and a construction method thereof, and belongs to the technical field of biology. The red yeast engineering bacterium is an xanthophyllomyces dendrorhous strain SXD, and is preserved in China General Microbiological Culture Collection Center, the preservation number is CGMCC No.10519, and the preservation date is February 4th, 2015. The xanthophyllomyces dendrorhous engineering bacterial strain SXD is obtained by a metabolic engineering method, and metabolic flux of astaxanthin synthesizing route of xanthophyllomyces dendrorhous is substantially improved. The highest astaxanthin content of the xanthophyllomyces dendrorhous engineering bacterial strain SXD is 4.4mg/g dry cell weight, after optimal cultivation, the astaxanthin content of the bacterial strain reaches 7.1mg/g dry cell weight, the maximum biomass is 83.8g/L, and an industrial exploitation prospect is provided. The SXD bacterial strain is not treated by any mutagenesis, the astaxanthin yield can be increased continuously and significantly by means of a mutation breeding, and production cost of yeast astaxanthin is hopeful to reduce continuously.

The invention relates to a strain for generating phospholipase D with high and stable yield by utilizing physical and chemical mutation, belonging to the technical field of microbial mutation. The strain is characterized in that the phospholipase D is prepared by fermenting streptomyces chromofuscus of high yield phospholipase D, which is obtained by taking the streptomyces chromofuscus as an original strain and sequentially carrying out composite mutation of ultraviolet rays and lithium chloride and composite mutation of atmosphere cold plasmas and the lithium chloride on a monospore bacterial suspension thereof. The invention has the advantages that the streptomyces chromofuscus of the high yield phospholipase D is obtained through mutation screening, thereby providing a high yield strain with higher application value to industrial production of the phospholipase D, laying a good foundation for fermentation culture and application of the phospholipase D, and also providing a good and feasible method for microbial mutation breeding. The invention adopts a mutation and screening method, has the advantages of simple operation, high efficiency and the like, and has a practical value on screening the strain with an industrial application value.

The invention relates to Trametes Versicolor YS-L613 and a breeding method and application thereof. The Trametes Versicolor YS-L613 is prepared by the method through mutation breeding. The breeding method is to take Trametes Versicolor as a starting strain, adopt composite mutation breeding technology which combines UV, 60 CO, ultraviolet rays, nitrosoguanidine, ion implantation and microwave treatment, and breed the YS-L613 strain for high-yield production of laccase. The invention performs breeding operation through physiochemical treatment methods such as ion implantation, the ultraviolet rays and mutagen, finally obtains the YS-L613 strain for high-yield production of the laccase, and adopts experimental automatic control technology, strain fermentation control technology and strain extraction technology to develop laccase products. As proved by experiments of the laccase in pulp bleaching, fiber modification and pulp deinking, utilization of the laccase in the paper-making industry can improve the quality of paper, reduce environmental pollution and save the cost.

The invention relates to a chemical mutation breeding technology in the field of biotechnologies, and particularly relates to an in-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium. The in-vitro NaN3 mutation breeding technology comprises the following steps of: performing culture in vitro on an immature stem, a stem tip or a leaf of the dendranthema morifolium to form an aseptic seedling; processing the stem tip and a stem segment of the aseptic seedling, which are taken as explants, by sodium azide (NaN3) in a breeding process to carry out induced mutation; rooting and transplanting a test-tube plantlet; and finally breeding excellent mutant in a field at a flowering phase. The in-vitro sodium azide (NaN3) mutation breeding technology can effectively overcome the obstacle of breed improvement of a clonal plant and the constraints of low bud-variation frequency and small mutational range under natural conditions, can be used for obtaining the excellent mutant within a short period of time, ensures high induced mutation efficiency, and has an important value to the genetic improvement of asexual propagation of the dendranthema morifolium with medicinal and ornamental values.

The invention discloses a bacillus subtilis capable of stably producing chymosins with high yield by mutation and an application. The original strain used is a bacillus subtilis which is obtained by the screening of a laboratory and used for producing products such as cheeses and the like by fermentation, and is preserved in China Center for Type Culture Collection (CCTCC) in February 2008, with the preservation name of bacillus subtilis QL-2 (Bacillus subtilis QL2) and the preservation No. of M208023. The mutation breeding comprises the following steps of: taking the bacillus subtilis QL-2 which is preserved in CCTCC with the culture preservation No. of M208023 as the original strain, and then culturing the bacillus subtilis QL-2 on a rejuvenation culture medium and a fermentation culture medium in sequence so as to obtain the original strain suspension; using a vortex device to make the strain suspension into single spore suspension; and then conducting ultraviolet mutation and culture for a plurality of times to the single spore suspension; and finally obtaining the bacillus subtilis YB-3 (Bacillus subtilis YB-3 , CCTCC NO: M209075) capable of stably producing chymosins with high yield. The bacillus subtilis capable of stably producing chymosins with high yield can be used for preparing chymosin crude enzymes by liquid fermentation, which can be used for preparing cheeses and caseins, and shortens the clotting time of milk.

The invention discloses a mutation breeding method for cunninghamia lanceolata. The method comprises the following steps: especially adopting ripen seeds of a cunninghamia lanceolata seed garden; carrying out low-temperature overwintering treatment, carrying out constant-temperature seed soaking stimulation on a gibberellin solution with specific concentration, immediately putting the gibberellin solution subjected to constant-temperature seed soaking stimulation into clear water, carrying out variable-temperature soaking for 3 days, and carrying out sprout promoting treatment; respectively carrying out colchicine mutation treatment, ethylmethane sulfonate (EMS) mutation treatment and sodium azide (NaN3) mutation treatment; and finally seeding and germinating the cunninghamia lanceolata seeds which are subjected to mutation treatment, so as to obtain a large group of cunninghamia lanceolata mutation seedlings. The cunninghamia lanceolata mutation seedlings obtained by the mutation method have the characteristics of obvious phenotypic characteristic variation, large population growth characteristic variation range and the like; a great number of mutated cunninghamia lanceolata materials can be formed within a short period of time by the method disclosed by the invention; and a foundation is laid for breeding of a new variety of cunninghamia lanceolata.

The invention discloses a mixed liquid fermentation process of three plants of bacillus licheniformis. According to the mixed liquid fermentation process, the three plants of the bacillus licheniformis, namely L1, L2 and L3 with different physiological functions, which are originally cultured under different conditions, are subjected to solid culture and shaking flask seed preparation by performing artificial mutation breeding and optimizing process formula and culture conditions, then shaking flask seed liquid of the three plants of the bacillus licheniformis is merged into a flask, first-stage seed liquid preparation and second-stage seed liquid preparation are performed, and fermentation culture is finally performed in a same fermentation tank. By adopting the mixed fermentation process disclosed by the invention, the bacteriostatic ability is improved by 10-15%, the sporation rate is improved by 16-23% and the cost is saved by 200%.

The invention relates to the technical field of microorganism mutation breeding, in particular to a high-yield glutamine transaminase strain. The high-yield glutamine transaminase strain (YRZ170817NTG31) is preserved in the China Center For Type Culture Collection and has the preservation number of 15353 and the preservation date of February 12th, 2018. The high-yield glutamine transaminase straincan has the maximum enzyme activity after 24 hours of seed culturing and 48-56 hours of fermentation culturing and has the enzyme activity of 20-40 U/mL. The strain has the advantage of being high inenzyme activity.

The invention discloses a strain for producing cellulase. The strain has a collection number of CGMCC No.8339 in the China General Microbiological Culture Collection Center, and the classification name of the strain is Penillium piceum H16. The Penillium piceum is obtained through induced mutation treatment by taking a strain Penillium piceum 9-3 screened from straws as an original strain. The Penillium piceum 9-3 serves as the original strain for mutation breeding, the Penillium piceum H16 for producing high-activity cellulase is obtained through breeding, the enzyme activity of filter paperof cellulase crude enzyme liquid obtained by fermenting the Penillium piceum H16 reaches 7IU/mL, and the enzyme activity of beta-glucosidase reaches 50IU/mL. Different cellulase systems of the Penillium piceum H16 and Trichoderma reesei RUT-C30 are researched according to different proportions, the enzyme activity and the hydrolysis rate of microcrystalline celluloses are greatly improved at an optimal proportion, and the enzyme cost is reduced.

The present invention relates to the technical field of microbial mutation breeding and particularly to a high-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and an application thereof. The lactobacillus plantarum LP-08 is deposited in Guangdong Microbial Culture Collection Center (GDMCC) on June 16, 2020 and has a deposit number of GDMCC No:61061. The present invention aimsto develop ground-origin green tea dregs to prepare functional bacterial tea biological feeds, an ARTP mutagenesis and domestication screening method is used to select functional microorganisms, and the lactobacillus plantarum LP-08 that can tolerate high-concentration tea dregs and is good in biomass and metabolite activity is obtained. The strain can be used for tea dreg fermentation, can changenutrient ingredients in tea dregs, can be applied to development of the tea dreg feeds, and is of great significance for high-value utilization of tea dreg resources.