8 results about "Mutation breeding" patented technology

The invention belongs to the technical field of biology, and particularly relates to a recombinant strain of high-yield riemerella anatipestifer bacteriophage lyase, a mutant strain, a construction method and application. The invention provides a coding gene of riemerella anatipestifer bacteriophage lyase, and further constructs recombinant lactococcus lactis of high-yield riemerella anatipestifer bacteriophage lyase. The mutant strain provided by the invention is obtained by taking the recombinant lactococcus lactis as a starting strain through a mutation breeding technology and a specific high-throughput screening strategy, has higher secretory expression quantity of riemerella anatipestifer bacteriophage lyase, is good in hereditary stability in an antibiotic-free culture medium, and has a good application prospect. The method is suitable for industrial efficient production of active lyase. The riemerella anatipestifer bacteriophage lyase expressed by the mutant strain has an obvious splitting effect on riemerella anatipestifer in vitro, has obvious prevention and treatment effects on riemerella anatipestifer infection in vivo, and can effectively improve the survival rate of infected chicks and remove pathogenic bacteria in vivo.

The utility model relates to the technical field of mutagenesis breeding of edible fungi, in particular to a soaking device for mutagenesis breeding of edible fungi, which comprises a fixed frame, a plurality of movable frames I are arranged side by side below the left side in the fixed frame, and a carrier roller I is movably mounted at the upper end of each movable frame I through a rotating shaft; a width adjusting mechanism is arranged between the fixed frame and the movable frame I; driving rollers are arranged on the left side of the interior of the fixing frame and close to the right end of the first movable frame side by side, and a transmission shell is fixedly installed outside the left side of the fixing frame. Through the synergistic effect of the lead screw width adjusting mechanism and the electric push rod length adjusting mechanism, the radial and axial dual self-adaptive adjusting function is achieved, the distance between the carrier rollers can be accurately controlled, the length tolerance of the test tubes can be automatically compensated, various test tubes with different diameters and lengths can be stably clamped in cooperation with the linkage design of the connecting rods and the springs, and the test tube clamping efficiency is improved. The technical bottleneck that a traditional device cannot be compatible with test tubes of multiple specifications is solved.

The application discloses application of marine actinomycetes of Saccharothrix sp. in improving heat resistance of algae and / or promoting growth of algae, and relates to the technical field of microbial mutation breeding. The marine actinomycetes of Saccharothrix sp. is screened by using a method of ultraviolet mutation. Compared with a wild type, the bacteria can obviously improve the high-temperature resistance of algae, and has the characteristics of promoting the growth of algae. It is indicated that the bacteria strain has good application prospect of improving the heat resistance of algae and / or promoting the growth of algae.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

The present application relates to the technical field of breeding, in particular to a method for improving the efficiency of banana mutation breeding, which comprises the steps of induction of differentiated buds, treatment of differentiated buds with growth regulators and light stress, irradiation mutation treatment, culture of differentiated buds, rooting of the treated seedlings, transplanting and germplasm screening. The method of the present application is beneficial to improve the efficiency of banana breeding and accelerate the breeding process.

The invention belongs to the field of biotechnology and microbial fermentation engineering, and particularly relates to a cryptococcus albidus strain and application thereof in production of sclareolide. A mutant strain with the most excellent performance is screened out through systematic wild strain screening, natural breeding, ARTP mutation breeding and other technical means, the mutant strain is named as Cryptococcus albidus FM1120, the preservation number is CCTCC NO: M 20251915, the preservation date is September 1, 2025, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915. The preservation unit is China Center for Type Culture Collection. The invention also provides an application of the strain FM1120 in the production of sclareolide, sclareol is taken as a substrate, the conversion rate of sclareol reaches more than 90% through fermentation, the fermentation liquor has the advantages of few impurities, easiness in separation and purification of sclareolide, high purity and the like, and a new path is provided for the green preparation of sclareolide.

The invention discloses a method for creating new germplasm by grafting potato tissue culture seedlings, relates to the technical field of potato breeding, and solves the problems of long creation period, high parent selection difficulty and high screening cost of traditional potato new germplasm in cross breeding, mutation breeding and the like. Comprising the steps of parent tissue culture seedling cultivation, pretreatment before grafting, tissue culture seedling grafting, grafted seedling callus culture, seedling hardening domestication and germplasm screening. The method shortens the stable period of the new germplasm character, is not limited by seasons, multiplies the breeding efficiency, realizes efficient creation of the new potato germplasm, and can improve the breeding survival rate and the germplasm creation efficiency.

The invention discloses rhodotorula torula OMK-105 and application thereof, the rhodotorula torula OMK-105 is preserved in the China Center for Type Culture Collection on December 18, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20252957. According to the present invention, the high-yield strain Rhodotorula toruloides OMK-105 is obtained through mutation breeding, such that the accumulation ratio of DTA in the total oil can be significantly increased, the metabolic pathway is optimized, the production process has characteristics of economy and sustainability, and the generation of by-products is reduced.