40 results about "Mutation breeding" patented technology

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The invention relates to the field of astragalus membranaceus breeding, and particularly discloses an astragalus membranaceus breeding method combining space mutagenesis with phenotype screening, which comprises the following steps: S1, seed pretreatment: space mutagenesis: carrying original seeds on a return satellite, exposing the original seeds on an orbit 200-400km away from the ground for 48-72 hours, and receiving a space radiation dose of 2-5Gy; disinfection treatment: soaking the mutagenized seeds in a 3-5% sodium hypochlorite solution for 15-20 minutes, and then washing the seeds with sterile water for 3 times; through the synergistic effect of space mutation and phenotype screening, the limitation that traditional mutation breeding is high in randomness is broken through, the directional screening efficiency of target characters is remarkably improved, and meanwhile stable transmission of excellent hereditary characters is guaranteed; through combination of multi-dimensional phenotypic indexes of root development, biomass accumulation and stress resistance, superior plants with high medicinal component enrichment capacity and wide adaptability are accurately locked, and the problem of comprehensive performance imbalance caused by single character screening is solved.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention belongs to the technical field of biology, and particularly relates to a recombinant strain of high-yield riemerella anatipestifer bacteriophage lyase, a mutant strain, a construction method and application. The invention provides a coding gene of riemerella anatipestifer bacteriophage lyase, and further constructs recombinant lactococcus lactis of high-yield riemerella anatipestifer bacteriophage lyase. The mutant strain provided by the invention is obtained by taking the recombinant lactococcus lactis as a starting strain through a mutation breeding technology and a specific high-throughput screening strategy, has higher secretory expression quantity of riemerella anatipestifer bacteriophage lyase, is good in hereditary stability in an antibiotic-free culture medium, and has a good application prospect. The method is suitable for industrial efficient production of active lyase. The riemerella anatipestifer bacteriophage lyase expressed by the mutant strain has an obvious splitting effect on riemerella anatipestifer in vitro, has obvious prevention and treatment effects on riemerella anatipestifer infection in vivo, and can effectively improve the survival rate of infected chicks and remove pathogenic bacteria in vivo.

The invention belongs to the technical field of mutation breeding, and particularly discloses a breeding method for inducing watermelon mutation by using EMS. The method comprises the following steps: collecting pollen by using a glucose solution, then treating by using an EMS-glucose solution, pollinating female flowers blooming on that day after treatment, uniformly spraying a forchlorfenuron solution with a proper concentration on the pollinated female flower ovary, harvesting and planting M0-generation seeds, obtaining watermelon EMS dominant mutation individuals in the M1 generation, harvesting and planting M1-generation seeds, obtaining watermelon EMS mutation groups with rich variation in the M2 generation, and finally obtaining the watermelon EMS mutation groups with rich variation. And preparing a breeding combination by utilizing the beneficial mutants. According to the invention, various mutants such as stress yellowing, green leaf color, withered leaf margin, male sterility, short vine, plant malformation, leaf malformation, peel and stripe variation, fruit flavor variation and the like can be obtained; the technical bottlenecks of narrow genetic background, shortage of excellent germplasm resources, low germplasm creation efficiency and the like in watermelon functional gene research and variety breeding are effectively solved, and the method can be used for creating excellent breeding new germplasm and cultivating new varieties.

The utility model relates to the technical field of mutagenesis breeding of edible fungi, in particular to a soaking device for mutagenesis breeding of edible fungi, which comprises a fixed frame, a plurality of movable frames I are arranged side by side below the left side in the fixed frame, and a carrier roller I is movably mounted at the upper end of each movable frame I through a rotating shaft; a width adjusting mechanism is arranged between the fixed frame and the movable frame I; driving rollers are arranged on the left side of the interior of the fixing frame and close to the right end of the first movable frame side by side, and a transmission shell is fixedly installed outside the left side of the fixing frame. Through the synergistic effect of the lead screw width adjusting mechanism and the electric push rod length adjusting mechanism, the radial and axial dual self-adaptive adjusting function is achieved, the distance between the carrier rollers can be accurately controlled, the length tolerance of the test tubes can be automatically compensated, various test tubes with different diameters and lengths can be stably clamped in cooperation with the linkage design of the connecting rods and the springs, and the test tube clamping efficiency is improved. The technical bottleneck that a traditional device cannot be compatible with test tubes of multiple specifications is solved.

The invention belongs to the technical field of bamboo reed breeding, and discloses a mutation breeding method for obtaining a bamboo reed cold-resistant mutant based on EMS (Enhanced Mass Spectrometry), which comprises the following steps: step 1, inducing embryogenic callus; step 2, carrying out EMS mutagenesis treatment; step 3, performing EMS stress screening; 4, differentiation and rooting culture of adventitious buds; 5, screening the tissue culture seedlings at low temperature; the mutation obtained through mutagenesis treatment is a permanent change of a genetic structure of an organism and may appear in germ cells and somatic cells of a plant. Physical or chemical mutagenic agents are used to increase the frequency of mutations. In chemical mutagens, EMS is considered to be the most effective mutagen, the EMS can generate a large number of non-lethal point mutations in the whole genome, the mutation frequency is high, the chromosome abnormality frequency is low, the EMS is used for treating embryogenic calluses of the bamboo reeds, Hyp is used for screening, the low-temperature-resistant bamboo reed mutants are obtained, the planting area of the bamboo reeds can be enlarged, and the survival rate of the bamboo reeds is increased. And waste land in a low-temperature region is fully utilized.

The present invention belongs to the technical field of microbial mutagenesis, and particularly relates to a microbial mutagenesis breeding device and its application. The microbial mutagenesis breeding device provided by the present invention includes a cube structure and a transmission structure. The cube structure is wrapped with light-impermeable materials around, and ultraviolet lamps are arranged inside. The transmission structure includes a single sorting area and a mutagenesis area. The mutagenesis area is inserted into the cube structure through the insertion opening of the cube structure and is arranged perpendicular to the ultraviolet lamps. Microorganisms can be sorted singly through the first horizontal spiral conveyor pipe in the single sorting area and transported to the second horizontal spiral conveyor pipe in the mutagenesis area, so that the time and intensity of the microorganisms being irradiated by ultraviolet light are uniform, significantly reducing the mutagenesis lethality rate of the microorganisms and significantly increasing the positive mutation rate. The operation is simple, the workload is small, and the cycle is short. Moreover, by treating the microorganisms with a protective agent having a specific composition before single sorting, the mutation uniformity and the positive mutation rate can be further improved.

The application discloses application of marine actinomycetes of Saccharothrix sp. in improving heat resistance of algae and / or promoting growth of algae, and relates to the technical field of microbial mutation breeding. The marine actinomycetes of Saccharothrix sp. is screened by using a method of ultraviolet mutation. Compared with a wild type, the bacteria can obviously improve the high-temperature resistance of algae, and has the characteristics of promoting the growth of algae. It is indicated that the bacteria strain has good application prospect of improving the heat resistance of algae and / or promoting the growth of algae.

The application discloses a kind of for plant seed neutron irradiation 241 Am- 9 Be source dose distribution irradiation device mainly includes cylindrical shield, transport plate, 241 Am- 9 Be neutron source, spherical shielding layer, rubber stick and sample irradiation area;The neutron source will be accompanied by a large number of 59.5keV, 4.438MeV gamma ray production while spontaneous fission, by wrapping spherical lead layer around neutron source shielding gamma ray, so that the main plant seed radiation effect comes from neutron radiation, while the geometric center of neutron source is the spherical center, set up sample irradiation area, ensure the uniformity of plant seed absorbed dose in each area, and directly given without going through tedious measurement and calculation, with the characteristics of standardization dose distribution, can meet the scientific research needs of most legume plant seed neutron irradiation, and can be applied to other kinds of plant seed irradiation and neutron radiation agricultural production and mutation breeding.

This invention discloses a salt-stress-tolerant Bacillus bellis and its applications. It relates to the field of agricultural microbiology. The salt-stress-tolerant Bacillus bellis ZY-2, with accession number CGMCC NO.34345, is described, along with its related applications. The salt-stress-tolerant Bacillus bellis provided by this invention has broad application prospects in resisting saline-alkali environments. Specifically, this strain can be used as the original material to breed strains with better tolerance to saline-alkali environments and more significant effects on promoting wheat growth and development through natural selection, artificial mutation breeding, and genetic engineering breeding, thereby reducing the use of chemical amendments. Furthermore, it exhibits strong tolerance to saline-alkali environments and demonstrates good promotion of wheat growth and development under salt stress.

The invention discloses a breeding device and method for mutagenizing flower seeds through ionizing radiation, and particularly relates to the technical field of flower mutagenizing breeding, the breeding device comprises a breeding box, a mutagenizing box is arranged in the breeding box, one end of the mutagenizing box is connected with a driving motor through a rotating shaft, the upper end of the mutagenizing box is provided with a mounting disc, and the upper end of the mounting disc is connected with a driving motor through a rotating shaft; a connecting shaft is fixedly arranged at the upper end of the mounting disc, the upper end of the connecting shaft is connected with a driving motor through a transmission mechanism, a discharging pipe is arranged at the bottom end of the breeding box, a feeding pipe is arranged at the end, away from the rotating shaft, of the breeding box, and a shielding layer is arranged on the inner side wall of the breeding box; feeding and discharging of flower seeds can be achieved without opening the breeding box, the structure is simple, operation is convenient, harm of ionizing radiation to the human body can be prevented, and the feeding and discharging speed of the flower seeds can be increased.

The invention relates to the technical field of plant breeding, and discloses an alfalfa space mutation breeding technology which comprises the following steps: performing space mutation treatment on alfalfa parent seeds to obtain SP0-generation seeds; determining a breeding target, and formulating a screening strategy; the SP0-generation seeds are sown, and an SP1-generation group is established; screening in the SP1-generation group or a progeny group of the SP1-generation group to obtain a variant single plant with a target variant character; carrying out evaluation and seed collection on the obtained variant single plant; and carrying out multi-generation systematic breeding and purification on the harvested seeds to obtain the new alfalfa strain with stably inherited target characters. According to the method disclosed by the invention, the seeds are treated through a space environment, and ground multi-generation system breeding is combined, so that a breakthrough alfalfa genetic material is efficiently created, and excellent mutation characters, such as multileaf and flower color variation, which are difficult to explore in original germplasm are obtained.

The invention discloses an apple rootstock space mutation breeding method which comprises the following steps: selecting full apple rootstock seeds, drying in the shade, and storing at 4 DEG C; the seeds are loaded on a return spacecraft and are in orbit for 7-180 days; after returning, grading and laminating according to hundred-grain weight: laminating less than or equal to 1.387 g for 46 days; 1.388 g to 2.746 g of the product is laminated for 53 d to 56 d; more than or equal to 2.747 g lamination is carried out for 56 days; the temperature is 2-4 DEG C, and the humidity is 60%; sowing single seeds in a nutrition pot after 50% of white buds appear, establishing a single-plant electronic file after seedling emergence, and recording seedling height, seedling thickness, leaf area, leaf form and images; in the last ten days of July, pruning branch sections with 3-4 buds, leaving one leaf at the top end, soaking in rooting liquid for 20-30 minutes, and cutting in vermiculite bowls with the humidity of more than or equal to 90%, the arched shed height of 2 m and the temperature of less than or equal to 30 DEG C; according to the apple rootstock space mutation breeding method, the breeding period is shortened, the mutant screening efficiency is improved, and the cutting rooting rate is increased.

The invention relates to the technical field of microorganisms, in particular to a polyhydroxyalkanoate engineering bacterium as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, selecting a PHA production strain, inoculating the PHA production strain to a culture medium, and performing amplification culture on a shaking table; 2, after culture is completed, a low-temperature plasma mutagenesis instrument is adopted for mutation breeding; 3, screening and separating the excellent mutant strains capable of producing PHA, and constructing a high-yield PHA mutant strain library; and 4, carrying out composite screening on the mutated mutant strain library, and selecting a high-yield PHA strain. According to the method for constructing the engineering bacteria capable of producing the PHA by adopting the novel small plasma mutagenesis instrument and the application of the engineering bacteria, the engineering bacteria capable of producing the PHA at high yield are constructed and screened through low-temperature plasma mutagenesis, so that the yield of the PHA is increased, and a new path is provided for production of a degradable biological material PHA.

The invention discloses a plasma mutation breeding method for callus of hybrid pennisetum alopecuroides' Bangdel No.1 '. According to the method, the callus induced by the Bangdel No.1 seed is used as a mutagenesis base material, an ARTP radiation mutagenesis technology is combined with plant tissue culture, and the mutagenesis effect of ARTP radiation on the hybrid pennisetum callus is explored from different aspects of phenotype, physiology and molecules in different stages of callus growth and plant regeneration. Furthermore, a low-temperature stress test is carried out on the regenerated plant, the morphological and physiological index differences of a mutagenesis plant and a control plant before and after low-temperature stress treatment are observed, mutagenesis strains with cold-resistant potential in the present generation are screened, and the cold-resistant hybrid pennisetum germplasm is obtained. The invention provides theoretical and material basis for the breeding of new germplasm of cold-resistant hybrid pennisetum alopecuroides.

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof. In order to enrich the source of strains with low-yield higher alcohols, the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is separated and screened from fermented grains of Luzhou-flavor liquor through primary screening and secondary screening, then normal-pressure plasma mutation breeding is performed on the Wickerhamomyces anomalus Wa9, and the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is obtained through primary screening and secondary screening. The Wickerhamomyces anomalus WA27 has the advantages that the Wickerhamomyces anomalus WA27 is high in yield, environmental tolerance and ethanol conversion rate, and the preservation number of the Wickerhamomyces anomalus WA27 is CCTCC NO: M 20252211. The strain not only maintains good ethanol yield, but also significantly reduces the generation of higher alcohols. And the strain is obtained through ARTP mutagenesis, is good in hereditary stability and is suitable for industrial production. When the prepared distiller's yeast is used for fermenting white spirit, a white spirit product with better flavor and taste can be obtained.

The invention relates to the field of biological breeding, and discloses a breeding method capable of enhancing breeding of ganoderma lucidum mushroom with anticancer activity, which comprises the following steps: S1, selecting a ganoderma lucidum mushroom female parent strain for pre-culture; s2, performing mutagenesis treatment on the hyphae; s3, performing character screening and liquid culture on the mutagenized strains to obtain mycelia; s4, carrying out extraction treatment on the mycelium to obtain polysaccharide and triterpenoid anticancer active ingredients, and carrying out quantitative analysis on the polysaccharide and triterpenoid anticancer active ingredients; s5, carrying out an in-vitro anticancer activity test on the extract of the strain, and evaluating the cell proliferation inhibition rate of the strain; and S6, screening out strains with enhanced anti-cancer activity and stable characters, and carrying out passage verification and industrial cultivation test. The anti-cancer activity enhanced mutant is obtained from the ganoderma lucidum mushroom strain through a mode of combining mutation breeding with function screening, so that the effects of improving the expression quantity of active ingredients and enhancing the cell inhibition effect are achieved, and the problems that the screening period is long and the activity is not obviously improved are solved.

The invention belongs to the technical field of crop mutation breeding, and particularly relates to a method for improving the EMS mutagenesis effect of oat. The method comprises the following steps: firstly, treating seeds by using concentrated sulfuric acid to remove hulls, adjusting the water content of the seeds to a suitable range, then screening an optimal ethylmethane sulfonate (EMS) mutagenesis condition, and finally, performing exogenous treatment by using gibberellin to promote germination of the mutagenized seeds and growth of seedlings. Planting the M0 generation in the generation adding room and harvesting the M1 generation seeds. On the basis, an Mgeneration mutant group is constructed, and the agronomic traits of the Mgeneration mutant group are investigated in a milk-ripe stage to evaluate the mutagenesis effect. By integrating seed hull peeling treatment, seed water content adjustment pretreatment, EMS mutagenesis treatment conditions and exogenous gibberellin composite treatment conditions, a set of EMS mutagenesis method capable of remarkably improving the survival rate of mutants and the richness of variation types is established, and reliable technical support is provided for germplasm innovation and variety breeding of the feeding oat.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

A strain for high-yield production of milbemycin and use thereof is disclosed. In the present invention, an original strain MI-W1-2017001 is subjected to mutation breeding by an atmospheric and room temperature plasma mutation method to obtain a high-yield strain HZMRYB-5. The fermentation level of the strain is improved by about 70% compared with the original strain, and the fermentation level is further improved by about 35% through optimization of a fermentation process and a formulation, so that the fermentation cost of the product is further reduced, and requirements for the industrial fermentation level are met. On the basis of optimizing the fermentation process, the ratio of A3 and A4 in a fermentation liquid is nearly 3:7 through mixed fermentation of the strain MI-W1-2017001 and the strain HZMRYB-5, so that market demands are greatly met, the process steps of separation, purification and then mixing are reduced.

The invention belongs to the technical field of breeding, and particularly provides a porphyra haitanensis-based mutation breeding method, which comprises the following steps: 1) carrying out ultraviolet radiation on a porphyra haitanensis filament; the invention discloses a method for breeding thalli through mutagenesis, which comprises the following steps of: 1) preparing protonema, 2) treating and irradiating the protonema with a mutagenic agent which comprises nitrosoguanidine and a slow-release solution which comprises bleomycin and pingyangmycin, and 3) screening the protonema subjected to mutagenesis treatment to obtain a mutant strain, and continuously culturing to obtain the thalli. The breeding method has the advantages of high efficiency, high mutation rate and good specificity.

The invention discloses cryptococcus albidus and application of the cryptococcus albidus in synthesis of norambrox ether, and belongs to the technical field of bioengineering. The preservation number of the cryptococcus albidus JMIC881 is CCTCC (China Center for Type Culture Collection) NO: M 20251916, the preservation date is September 1, 2025, and the preservation unit is the China Center for Type Culture Collection. The strain JMIC881 is separated from clary sage flowers, and is a microbial strain obtained by natural breeding and ARTP mutation breeding of wild cryptococcus light white yeast. The invention also provides a method for producing norambrox ether by using sclareol as a substrate through fermentation by using the strain JMIC881, and the method has the advantages of mild reaction conditions, no chemical pollutant emission, high product selectivity, low energy consumption and the like, solves the environmental problem caused by the traditional chemical synthesis method, reduces the equipment cost and energy consumption of industrial production, and is suitable for industrial production. And a new path is provided for green preparation of the ambrogyl ether.

The utility model discloses a mango ultraviolet mutation breeding device, which relates to the technical field of mango planting and comprises a support frame, a light shield and an ultraviolet lamp. The supporting frame supports the light shield from the inner side or the outer side. And the light shield is made of a non-transparent material and is used for covering the mango trees. The ultraviolet lamp is installed on the inner side of the light shield and used for irradiating ultraviolet light to male and female gametes of mangoes. Compared with the prior art, the mango ultraviolet mutation breeding device disclosed by the utility model is used for mutagenizing male and female gametes of mangoes at the same time under the condition of normal growth of mango trees, and is simple in structure, convenient to operate and good in universality.

The present application relates to the technical field of breeding, in particular to a method for improving the efficiency of banana mutation breeding, which comprises the steps of induction of differentiated buds, treatment of differentiated buds with growth regulators and light stress, irradiation mutation treatment, culture of differentiated buds, rooting of the treated seedlings, transplanting and germplasm screening. The method of the present application is beneficial to improve the efficiency of banana breeding and accelerate the breeding process.

The invention discloses a device for mutation breeding of colored zantedeschia aethiopica, and relates to the technical field of polyploidy induction, and the device is technically characterized by comprising an incubator and a box cover rotationally connected to the incubator, a controller with a timing function is fixedly mounted on the box cover, and an induction factor supplementing structure is arranged on the incubator; the induction factor supplementing structure comprises a rotating groove formed in the culture box, and an inner cavity of the rotating groove is rotationally connected with a hollow mounting frame. By arranging the rotatable multifunctional induction factor supplementing structure, collaborative precise regulation and control of temperature and gas / humidity are achieved, independence and stability of environmental parameters are guaranteed by utilizing a dynamic sealing mechanism, and the stability of the environment parameters is improved. And the closed-loop management of the induction process is realized by integrating multiple sensors and a timing controller, so that the problem that the traditional equipment only controls the temperature and ignores other induction factors is solved, and the synergy and data reliability of polyploidy induction are remarkably improved.

The application discloses a bacillus velezensis serine protease variant and application thereof. The serine protease variant is obtained by sequentially mutating a plurality of amino acid residues in a parent serine protease amino acid sequence, and still maintains the ability of the parent serine protease to hydrolyze a peptide bond; the amino acid sequence homology of the two reaches more than 95%. The serine protease variant provided by the application has higher catalytic activity after being incubated for 4 hours under an acid condition of pH 2-5 and for 12 hours under an acid condition of pH 2.2. The serine protease mutant provided by the application has better in-vitro enzymolysis effect on piglet feed than the original protease under the conditions of pH 2.2, a temperature of 40 DEG C, different enzyme addition amounts, different enzymolysis time and different solid-liquid ratios. The serine protease mutant provided by the application has good acid resistance, heat stability and excellent feed enzymolysis effect. When the enzyme addition amount is 400 U, the enzymolysis time is 4 hours, and the solid-liquid ratio is 0.7 g / mL, the enzymolysis benefit of the mutant enzyme is higher than that of other groups.

This invention discloses a method for blueberry radiation-induced mutation breeding. The method includes the following steps: 1) 60 Co-γ rays are used to irradiate tissue culture seedlings grown in culture medium A to obtain explant material; wherein the intensity of the irradiation treatment is 0.5-1.5 Gy / min, and the dose of the irradiation treatment is 80-120 Gy; 2) the explant material is inoculated into culture medium B for a first light culture to induce the generation of adventitious buds; 3) the culture from step 2) is inoculated into culture medium A for a second light culture to obtain regenerated seedlings; 4) the regenerated seedlings are transplanted into culture medium C for a third light culture to obtain blueberry plants. This invention utilizes radiation mutagenesis in blueberries to obtain a large number of irradiated blueberry plants within 3-4 months, providing technical support for blueberry breeding and improvement.

The present invention provides a soybean heavy ion beam radiation mutagenesis method, which belongs to the field of crop breeding technology. The soybean mutagenesis breeding method of the present invention comprises the following steps: (1) 12 C 6+ (1) irradiating soybean seeds with a radiation source of 40-60 Gy and a radiation time of 100-140 seconds; (2) germinating and culturing the irradiated soybean seeds using a culture medium; the culture medium is supplemented with 10-50 mg / L of vitamin C and 10-30 g / L of mannitol; and (3) transplanting the irradiated soybean seeds into a seedling culture medium after emergence for seedling cultivation, and spraying a water-soluble fertilizer containing 0.1%-0.3% seaweed extract and 0.5%-2% chitin during the seedling cultivation period. The present invention improves the mutant seed germination rate and seedling survival rate by adjusting the radiation dose and post-irradiation management method, promotes the stable expression of the mutant trait, accelerates the breeding process, and improves breeding efficiency.

The invention discloses a method for cultivating a new variety of hippeastrum hippeastrum leaves by using a somatic embryo and callus mixed regeneration way. The efficient cultivation method for the novel hippeastrum hippeastrum leaf variety is established through a somatic embryo and callus mixed regeneration way, and the breeding efficiency and the success rate of the novel hippeastrum hippeastrum leaf variety are remarkably improved by optimizing the whole process of tender leaf induction, embryogenic callus culture, somatic embryo induction and proliferation and germination and seedling formation; a new thought is provided for leaf color mutation breeding of the hippeastrum rutilum. The invention provides an efficient and stable technical scheme for rapid cultivation of a new variety of hippeastrum hippeastrum leaves.