21 results about "Mutation breeding" patented technology

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention belongs to the technical field of biology, and particularly relates to a recombinant strain of high-yield riemerella anatipestifer bacteriophage lyase, a mutant strain, a construction method and application. The invention provides a coding gene of riemerella anatipestifer bacteriophage lyase, and further constructs recombinant lactococcus lactis of high-yield riemerella anatipestifer bacteriophage lyase. The mutant strain provided by the invention is obtained by taking the recombinant lactococcus lactis as a starting strain through a mutation breeding technology and a specific high-throughput screening strategy, has higher secretory expression quantity of riemerella anatipestifer bacteriophage lyase, is good in hereditary stability in an antibiotic-free culture medium, and has a good application prospect. The method is suitable for industrial efficient production of active lyase. The riemerella anatipestifer bacteriophage lyase expressed by the mutant strain has an obvious splitting effect on riemerella anatipestifer in vitro, has obvious prevention and treatment effects on riemerella anatipestifer infection in vivo, and can effectively improve the survival rate of infected chicks and remove pathogenic bacteria in vivo.

The utility model relates to the technical field of mutagenesis breeding of edible fungi, in particular to a soaking device for mutagenesis breeding of edible fungi, which comprises a fixed frame, a plurality of movable frames I are arranged side by side below the left side in the fixed frame, and a carrier roller I is movably mounted at the upper end of each movable frame I through a rotating shaft; a width adjusting mechanism is arranged between the fixed frame and the movable frame I; driving rollers are arranged on the left side of the interior of the fixing frame and close to the right end of the first movable frame side by side, and a transmission shell is fixedly installed outside the left side of the fixing frame. Through the synergistic effect of the lead screw width adjusting mechanism and the electric push rod length adjusting mechanism, the radial and axial dual self-adaptive adjusting function is achieved, the distance between the carrier rollers can be accurately controlled, the length tolerance of the test tubes can be automatically compensated, various test tubes with different diameters and lengths can be stably clamped in cooperation with the linkage design of the connecting rods and the springs, and the test tube clamping efficiency is improved. The technical bottleneck that a traditional device cannot be compatible with test tubes of multiple specifications is solved.

The application discloses application of marine actinomycetes of Saccharothrix sp. in improving heat resistance of algae and / or promoting growth of algae, and relates to the technical field of microbial mutation breeding. The marine actinomycetes of Saccharothrix sp. is screened by using a method of ultraviolet mutation. Compared with a wild type, the bacteria can obviously improve the high-temperature resistance of algae, and has the characteristics of promoting the growth of algae. It is indicated that the bacteria strain has good application prospect of improving the heat resistance of algae and / or promoting the growth of algae.

The application discloses a kind of for plant seed neutron irradiation 241 Am- 9 Be source dose distribution irradiation device mainly includes cylindrical shield, transport plate, 241 Am- 9 Be neutron source, spherical shielding layer, rubber stick and sample irradiation area;The neutron source will be accompanied by a large number of 59.5keV, 4.438MeV gamma ray production while spontaneous fission, by wrapping spherical lead layer around neutron source shielding gamma ray, so that the main plant seed radiation effect comes from neutron radiation, while the geometric center of neutron source is the spherical center, set up sample irradiation area, ensure the uniformity of plant seed absorbed dose in each area, and directly given without going through tedious measurement and calculation, with the characteristics of standardization dose distribution, can meet the scientific research needs of most legume plant seed neutron irradiation, and can be applied to other kinds of plant seed irradiation and neutron radiation agricultural production and mutation breeding.

This invention discloses a salt-stress-tolerant Bacillus bellis and its applications. It relates to the field of agricultural microbiology. The salt-stress-tolerant Bacillus bellis ZY-2, with accession number CGMCC NO.34345, is described, along with its related applications. The salt-stress-tolerant Bacillus bellis provided by this invention has broad application prospects in resisting saline-alkali environments. Specifically, this strain can be used as the original material to breed strains with better tolerance to saline-alkali environments and more significant effects on promoting wheat growth and development through natural selection, artificial mutation breeding, and genetic engineering breeding, thereby reducing the use of chemical amendments. Furthermore, it exhibits strong tolerance to saline-alkali environments and demonstrates good promotion of wheat growth and development under salt stress.

The invention relates to the technical field of plant breeding, and discloses an alfalfa space mutation breeding technology which comprises the following steps: performing space mutation treatment on alfalfa parent seeds to obtain SP0-generation seeds; determining a breeding target, and formulating a screening strategy; the SP0-generation seeds are sown, and an SP1-generation group is established; screening in the SP1-generation group or a progeny group of the SP1-generation group to obtain a variant single plant with a target variant character; carrying out evaluation and seed collection on the obtained variant single plant; and carrying out multi-generation systematic breeding and purification on the harvested seeds to obtain the new alfalfa strain with stably inherited target characters. According to the method disclosed by the invention, the seeds are treated through a space environment, and ground multi-generation system breeding is combined, so that a breakthrough alfalfa genetic material is efficiently created, and excellent mutation characters, such as multileaf and flower color variation, which are difficult to explore in original germplasm are obtained.

The invention discloses an apple rootstock space mutation breeding method which comprises the following steps: selecting full apple rootstock seeds, drying in the shade, and storing at 4 DEG C; the seeds are loaded on a return spacecraft and are in orbit for 7-180 days; after returning, grading and laminating according to hundred-grain weight: laminating less than or equal to 1.387 g for 46 days; 1.388 g to 2.746 g of the product is laminated for 53 d to 56 d; more than or equal to 2.747 g lamination is carried out for 56 days; the temperature is 2-4 DEG C, and the humidity is 60%; sowing single seeds in a nutrition pot after 50% of white buds appear, establishing a single-plant electronic file after seedling emergence, and recording seedling height, seedling thickness, leaf area, leaf form and images; in the last ten days of July, pruning branch sections with 3-4 buds, leaving one leaf at the top end, soaking in rooting liquid for 20-30 minutes, and cutting in vermiculite bowls with the humidity of more than or equal to 90%, the arched shed height of 2 m and the temperature of less than or equal to 30 DEG C; according to the apple rootstock space mutation breeding method, the breeding period is shortened, the mutant screening efficiency is improved, and the cutting rooting rate is increased.

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof. In order to enrich the source of strains with low-yield higher alcohols, the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is separated and screened from fermented grains of Luzhou-flavor liquor through primary screening and secondary screening, then normal-pressure plasma mutation breeding is performed on the Wickerhamomyces anomalus Wa9, and the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is obtained through primary screening and secondary screening. The Wickerhamomyces anomalus WA27 has the advantages that the Wickerhamomyces anomalus WA27 is high in yield, environmental tolerance and ethanol conversion rate, and the preservation number of the Wickerhamomyces anomalus WA27 is CCTCC NO: M 20252211. The strain not only maintains good ethanol yield, but also significantly reduces the generation of higher alcohols. And the strain is obtained through ARTP mutagenesis, is good in hereditary stability and is suitable for industrial production. When the prepared distiller's yeast is used for fermenting white spirit, a white spirit product with better flavor and taste can be obtained.

The invention belongs to the technical field of crop mutation breeding, and particularly relates to a method for improving the EMS mutagenesis effect of oat. The method comprises the following steps: firstly, treating seeds by using concentrated sulfuric acid to remove hulls, adjusting the water content of the seeds to a suitable range, then screening an optimal ethylmethane sulfonate (EMS) mutagenesis condition, and finally, performing exogenous treatment by using gibberellin to promote germination of the mutagenized seeds and growth of seedlings. Planting the M0 generation in the generation adding room and harvesting the M1 generation seeds. On the basis, an Mgeneration mutant group is constructed, and the agronomic traits of the Mgeneration mutant group are investigated in a milk-ripe stage to evaluate the mutagenesis effect. By integrating seed hull peeling treatment, seed water content adjustment pretreatment, EMS mutagenesis treatment conditions and exogenous gibberellin composite treatment conditions, a set of EMS mutagenesis method capable of remarkably improving the survival rate of mutants and the richness of variation types is established, and reliable technical support is provided for germplasm innovation and variety breeding of the feeding oat.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

The invention discloses cryptococcus albidus and application of the cryptococcus albidus in synthesis of norambrox ether, and belongs to the technical field of bioengineering. The preservation number of the cryptococcus albidus JMIC881 is CCTCC (China Center for Type Culture Collection) NO: M 20251916, the preservation date is September 1, 2025, and the preservation unit is the China Center for Type Culture Collection. The strain JMIC881 is separated from clary sage flowers, and is a microbial strain obtained by natural breeding and ARTP mutation breeding of wild cryptococcus light white yeast. The invention also provides a method for producing norambrox ether by using sclareol as a substrate through fermentation by using the strain JMIC881, and the method has the advantages of mild reaction conditions, no chemical pollutant emission, high product selectivity, low energy consumption and the like, solves the environmental problem caused by the traditional chemical synthesis method, reduces the equipment cost and energy consumption of industrial production, and is suitable for industrial production. And a new path is provided for green preparation of the ambrogyl ether.

The present application relates to the technical field of breeding, in particular to a method for improving the efficiency of banana mutation breeding, which comprises the steps of induction of differentiated buds, treatment of differentiated buds with growth regulators and light stress, irradiation mutation treatment, culture of differentiated buds, rooting of the treated seedlings, transplanting and germplasm screening. The method of the present application is beneficial to improve the efficiency of banana breeding and accelerate the breeding process.

The application discloses a bacillus velezensis serine protease variant and application thereof. The serine protease variant is obtained by sequentially mutating a plurality of amino acid residues in a parent serine protease amino acid sequence, and still maintains the ability of the parent serine protease to hydrolyze a peptide bond; the amino acid sequence homology of the two reaches more than 95%. The serine protease variant provided by the application has higher catalytic activity after being incubated for 4 hours under an acid condition of pH 2-5 and for 12 hours under an acid condition of pH 2.2. The serine protease mutant provided by the application has better in-vitro enzymolysis effect on piglet feed than the original protease under the conditions of pH 2.2, a temperature of 40 DEG C, different enzyme addition amounts, different enzymolysis time and different solid-liquid ratios. The serine protease mutant provided by the application has good acid resistance, heat stability and excellent feed enzymolysis effect. When the enzyme addition amount is 400 U, the enzymolysis time is 4 hours, and the solid-liquid ratio is 0.7 g / mL, the enzymolysis benefit of the mutant enzyme is higher than that of other groups.

This invention discloses a method for blueberry radiation-induced mutation breeding. The method includes the following steps: 1) 60 Co-γ rays are used to irradiate tissue culture seedlings grown in culture medium A to obtain explant material; wherein the intensity of the irradiation treatment is 0.5-1.5 Gy / min, and the dose of the irradiation treatment is 80-120 Gy; 2) the explant material is inoculated into culture medium B for a first light culture to induce the generation of adventitious buds; 3) the culture from step 2) is inoculated into culture medium A for a second light culture to obtain regenerated seedlings; 4) the regenerated seedlings are transplanted into culture medium C for a third light culture to obtain blueberry plants. This invention utilizes radiation mutagenesis in blueberries to obtain a large number of irradiated blueberry plants within 3-4 months, providing technical support for blueberry breeding and improvement.

The invention belongs to the field of biotechnology and microbial fermentation engineering, and particularly relates to a cryptococcus albidus strain and application thereof in production of sclareolide. A mutant strain with the most excellent performance is screened out through systematic wild strain screening, natural breeding, ARTP mutation breeding and other technical means, the mutant strain is named as Cryptococcus albidus FM1120, the preservation number is CCTCC NO: M 20251915, the preservation date is September 1, 2025, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915, and the preservation number is CCTCC NO: M 20251915. The preservation unit is China Center for Type Culture Collection. The invention also provides an application of the strain FM1120 in the production of sclareolide, sclareol is taken as a substrate, the conversion rate of sclareol reaches more than 90% through fermentation, the fermentation liquor has the advantages of few impurities, easiness in separation and purification of sclareolide, high purity and the like, and a new path is provided for the green preparation of sclareolide.

The invention discloses a method for creating new germplasm by grafting potato tissue culture seedlings, relates to the technical field of potato breeding, and solves the problems of long creation period, high parent selection difficulty and high screening cost of traditional potato new germplasm in cross breeding, mutation breeding and the like. Comprising the steps of parent tissue culture seedling cultivation, pretreatment before grafting, tissue culture seedling grafting, grafted seedling callus culture, seedling hardening domestication and germplasm screening. The method shortens the stable period of the new germplasm character, is not limited by seasons, multiplies the breeding efficiency, realizes efficient creation of the new potato germplasm, and can improve the breeding survival rate and the germplasm creation efficiency.

The invention discloses rhodotorula torula OMK-105 and application thereof, the rhodotorula torula OMK-105 is preserved in the China Center for Type Culture Collection on December 18, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20252957. According to the present invention, the high-yield strain Rhodotorula toruloides OMK-105 is obtained through mutation breeding, such that the accumulation ratio of DTA in the total oil can be significantly increased, the metabolic pathway is optimized, the production process has characteristics of economy and sustainability, and the generation of by-products is reduced.

The invention belongs to the field of microorganisms, and relates to a high-yield tocopherol fermentation strain and application thereof in coarse cereal fermentation. The invention provides a strain M.rbg-2M, and the strain M.rbg-2M is obtained by carrying out ultraviolet mutation breeding on an original strain M.rbg. The strain forms a form beneficial to enrichment of tocopherol after mutagenesis, and fermentation verifies that the enrichment amounts of total tocopherol and alpha-tocopherol produced by the strain are 1.21 times and 1.93 times higher than those of an original strain respectively. Meanwhile, a high-quality coarse cereal substrate capable of promoting the high yield of tocopherol is selected and inoculated with M.rbg-2M for fermentation, and the total tocopherol content is increased by 3.2 times or above compared with that of a CK group. The growth and metabolism of fermentation strains are regulated and controlled by utilizing the metabolism characteristics of microorganisms, the metabolic flux is effectively increased, the enrichment amount of metabolites is increased, the optimal fermentation period and the optimal active matter harvesting time are determined, and at the moment, alpha-tocopherol, beta-tocopherol, gamma-tocopherol and delta-tocopherol are obviously increased compared with those before optimization. The total tocopherol enrichment amount is more than 15 times of the total tocopherol enrichment amount in the early stage of research, and finally high-efficiency and high-amount enrichment of total tocopherol and abundant tocopherol types are realized. And an excellent strain, a feasible scheme and technical parameters are provided for industrial production of tocopherol through monascus fermentation and enrichment.

The application provides a ray irradiator for fruit tree branch mutation breeding, which comprises a cabin, a ray irradiator and an irradiation work area arranged in the cabin, and a linear driver with one end extending into the irradiation work area and the other end located outside the cabin. A branch fixer is moved into the irradiation work area by the linear driver, and a lead block is arranged on the cabin for the branch fixer to pass through, which seals the cabin. The branch fixer comprises a rotary driver, and an output end of the rotary driver is provided with a rotating shaft. Two fixed discs are spacedly and key-connected to the rotating shaft. Each fixed disc is provided with embedding holes, and the embedding holes on each fixed disc are distributed in the circumferential direction. Two ends of the branch are respectively inserted into the embedding holes of the two fixed discs. The rotary driver drives the fixed discs to rotate, so that each branch is sequentially directed to the ray irradiator. The application has the advantages of compact structure, convenient use, low maintenance cost, high branch irradiation uniformity, controllable irradiation dose and good repeatability.