Induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos

Abstract

The invention provides an induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos, comprising the following steps: (1) building a regeneration system of a Photinia fraseri asepsis test tube seedling; (2) building a high-frequency rapid-propagation system induced by an in-vitro somatic embryo and an adventitious bud; and (3) rooting, acclimatizing and transplanting for the test tube seedling. The induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos is characterized in that coordinated sets of measures of selecting a proper explant, improving a basic culture medium and ingredients, regulating culture conditions and the like are adopted, and the Photinia fraseri of which the seed is difficult to breed is subjected to path rapid propagation by the somatic embryos; the plant transplantation survival rate is higher than 95%; the germchit grows strongly to solve the problem that an excellent germchit quality degenerates, and a great quantity of purified and rejuvenated detoxification nursery stock with good quality can be provided for production; and in addition, and ideal receptor system is provided for breeding new specimens for Photinia fraseri by genetic transformation or mutation breeding.

Description

technical field [0001] The invention relates to a method for inducing rapid propagation of somatic embryos from in vitro leaves of Photinia fragrans, belonging to the field of botany. Background technique [0002] Photinia ( Photiniaⅹfrasery ) is a hybrid of Photinia glabra (P.glabra) and Photinia (P.serrulata), which belongs to the evergreen broad-leaved small trees or multi-branched shrubs of the genus Photinia in the Rosaceae family. It is widely distributed in Japan, the United States and Europe. Because its new shoots and young leaves are as bright as fire, bright and long-lasting, with strong germination after pruning and compact plant shape, it is often used as a high-grade ribbon in garden landscapes; or it can be cultivated as a single-stemmed, spherical crown, and planted alone in the green space; or As a street tree; or potted and placed in the porch or indoors, the effect is good. It is a garden plant with high ornamental value. [0003] Since Photinia fragrans...

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Problems solved by technology

[0003]Since Photinia fragrans was introduced into China in the 1990s, it has been listed as a colorful tree species with high development value, but Photinia frondosa is different from many original Compared with tree species, the provenance in our country is scarce, and there has been no report of fruiting so far, and the excellent varieties introduced from abroad are also susceptible to fungal diseases such as leaf spot due to long-term cutting propagation, and they are also vulnerable to aphids in spring and summer. , the virus is serious, the leaf spots are easy to fall off, the ornamental value is reduced, and the degree of improved varieties is reduced; and the use of inferior seedlings leads to the loss of the inherent excellent characteristics of Photinia fragrans, which to a certain extent restricts the development and promotion of this tree species Therefore, the selection and breeding of excellent Photinia strains has become a very prominent production problem. Tissue culture and rapid propagation is an effective way for the industrialization of Photinia improved varieties, and there are many reports on related aspects, but the direct induction of Photinia leaves There are no successful reports on the technical research of somatic embryos and regenerative rapid propagation systems

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