A complete medium for inducing rapid propagation of somatic embryos from isolated leaves of Sophora japonica

A technology of isolated leaves and culture medium, applied in application, horticulture, botanical equipment and methods, etc., can solve the problems of less budding, long induction time of immature zygotic embryos, and low regeneration rate, and achieve the effect of saving time.

Active Publication Date: 2017-09-15
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcoming that above-mentioned method exists: because Chinese Sophora japonica is a perennial woody plant, its regeneration rate is generally low, and budding is little, has directly influenced the genetic transformation of Chinese Sophora japonica
However, in the Shandong area, the small peaks of Huaihua japonica have been seriously damaged in recent years, and it is difficult to collect intact seeds without pests
Secondly, compared with the induction of Sophora japonica leaves, the induction time of immature zygotic embryos is longer, and the number of clustered buds induced is not as much as that induced by leaves.

Method used

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  • A complete medium for inducing rapid propagation of somatic embryos from isolated leaves of Sophora japonica
  • A complete medium for inducing rapid propagation of somatic embryos from isolated leaves of Sophora japonica
  • A complete medium for inducing rapid propagation of somatic embryos from isolated leaves of Sophora japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0037]Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Three stem segments were inoculated in each bottle to induce the germination of terminal buds and axillary buds. After 15 days of cultivation, the axillary buds or terminal buds of the stems begin to ger...

Embodiment 2

[0049] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0050] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 40 seconds, rinse with sterile water for 4 times, then sterilize with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 18 days of cultivation, the axillary buds or terminal buds of the stems begin to germinate, and the stem tips elongate obviously, an...

Embodiment 3

[0062] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0063] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 60 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercury chloride for 15 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 20 days of cultivation, the axillary buds or terminal buds of the stems begin to germinate, and the stem tips elongate obviously, and...

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Abstract

The invention discloses a kit culture medium for detached-leaf-somatic-embryo induced rapid propagation of sophora japonica. The kit culture medium comprises a bud starting culture medium, a test-tube plantlet propagation culture medium, a somatic embryo induction culture medium, an adventitious bud induction culture medium, a sound seedling culture medium and a rooting culture medium. Through propagating sophora japonica seedlings by using the culture medium disclosed by the invention, the regeneration rate is high, the budding is good, the survival rate of plant transplanting can reach 95% or more, and the seed seedlings are robust in growth, so that the problem of germ plasm degeneration of excellent seed seedlings can be effectively solved, and an ideal acceptor system can be provided for the genetic transformation and induced breeding of the sophora japonica.

Description

technical field [0001] The invention relates to a complete set of culture medium for inducing rapid propagation of somatic embryos of isolated leaves of Sophora japonica. Background technique [0002] Sophora japonica Linn, also known as Sophora japonica Linn, also known as Sophora japonica Linn, belongs to Papilionaceae and Sophora genus. Chinese pagoda tree is a deciduous tree with beautiful tree vigor, long flowering period, extremely resistant to pruning, cold resistance, drought resistance, and air pollution resistance. It is also a symbol of auspiciousness, happiness and beauty. It is an ideal tree species for street and garden greening. The material is excellent, the flowers, fruits, root bark, and bark can be used as medicine, and the seeds can be squeezed to make soap. It has high economic value and has a very broad application prospect. At present, the national pagoda tree is the only rootstock tree species for grafting ornamental species such as Sophora japonica,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 李双云刘盛芳李自成刘桂民安连任冯建华夏阳庞彩红
Owner SHANDONG FOREST SCI RES INST
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