366 results about "Rootstock" patented technology

The invention provides a method for grafting Tibetan white bark pine on Chinese pine, belongs to the technical field of needle-leaved tree species grafting, and solves the technical problem that the survival rate is lower than 40% due to slow joint healing, high pathogen infection rate and environmental fluctuation. According to the technical scheme, the method is characterized by comprising the following steps: cutting 1.8-2.2 cm of a vertical notch reaching xylem at the part, which is 2.5-3.5 cm exposed out of the surface of a culture medium, of a rootstock rhizome part; cutting the scion base into a symmetrical double-sided wedge shape, and immersing the scion base into a sodium citrate buffer solution containing polyvinylpyrrolidone, methyl salicylate and calcium chloride; the two sides of the vertical notch are coated with adsorption glue; inserting a scion alignment cambium and fixing by using a grafting belt; an ethanol water solution containing salicylic acid, trehalose and polysorbate 80 is applied to the interface every 2-3 days; maintaining the day temperature to be 23-25 DEG C, the night temperature to be 16-18 DEG C and the humidity to be 90-95%; when the young sprouts grow to 2-3 cm, the rootstocks are girdled, and trunks are cut off after 7-10 days. The method is used for large-scale breeding of the Tibetan pinus bungeana grafted seedlings.

This invention relates to the field of bell pepper seedling transplanting technology, specifically a fully automatic mobile tray-type bell pepper seedling transplanting and planting integrated machine, including a trolley, a machine vision module, a tray handling module, a tray storage module, a tray seedling removal module, a seedling picking module, a planting module, and a soil gathering and irrigation module. In most bell pepper planting processes, manual transplanting is primarily used, where seedlings cultivated in trays are manually transported to the field and then transplanted manually. Some machines rely on manual tray handling, using mechanical claws to directly grasp the rootstock, causing damage to the seedling roots. This integrated machine achieves full automation without damaging the seedling roots, using a linkage to press and remove the seedlings, greatly ensuring the integrity of the seedling roots. Multiple trays can be planted simultaneously, and the removed trays can be collected and processed uniformly, significantly improving planting efficiency and accuracy, reducing manual labor input, and increasing seedling survival rate.

The invention relates to the technical field of plant grafting, in particular to a method for improving the grafting survival rate of malania oleifera, which comprises the following steps: S1, scion preparation: treating branches of excellent perennial wild malania oleifera plants in the field, conveying the branches to an inoculation field, and disinfecting to obtain disinfected branches; s2, stock preparation; s3, grafting: preparing a scion by using the disinfected branch, treating a stock, tightening the stock and a grafting film for tongue grafting of the scion, and smearing a healing agent on a cut of the cut stock and the upper end of the grafting film; s4, management after grafting. Compared with the prior art, the method has the advantages that superior plant branches and artificially cultivated stocks are subjected to tongue grafting, and compared with bud grafting, the yield time of grafted seedlings is shortened; and then the branch of the malania oleifera superior plant is treated immediately when the scion is collected, so that the problems that the activity of the branch is reduced in the transportation process and the formation of the callus of the stock and scion cambium is slow are solved, the grafting survival rate is increased, and the grafted seedling obtaining time is shortened.

The invention discloses a Ilex rotunda grafting method which comprises the following steps: from late February to mid-March, preparing a 3-5-year-old Ilex rotunda rootstock for cultivation, and irrigating the Ilex rotunda rootstock; preparing a scion with double inclined planes; cutting a cut with the depth of 3cm in the rootstock, inserting the scion and aligning to the cambium; and carrying out double-layer binding by using a viscous PVC grafting film and a grafting soft film. The final-period management comprises the steps of sunshelter building, irrigation, bud picking, unbinding, pinching, fertilization and weeding. The problem that Ilex rotunda is difficult to breed is solved, the grafting survival rate is high, and the seedling raising period is short.

The invention relates to the technical field of fine processing of agricultural products, and provides a method and a system for quantitatively distinguishing peels of soft-branch stock oranges and processing type oranges, and the method comprises the following steps: S1 to S4, selecting two types of mature oranges, freeze-drying and grinding peels, and mixing the peels according to a preset proportion to obtain peel residues; weighing the same amount of the sample, adding methanol, and carrying out ultrasonic extraction, centrifugation and filtration by a filter membrane to obtain a to-be-detected solution; and calculating the proportion of the polyoxymethylene flavone glycoside in the soft-branch rootstock orange peel through HPLC (High Performance Liquid Chromatography) detection by combining an integral peak area of a standard curve of the polyoxymethylene flavone glycoside. The invention further discloses a system for executing the method. According to the method, the polyoxymethylene flavone glycoside is used as an index, HPLC detection and standard curve calculation are combined, whether the fruit peel is contained or not can be clearly distinguished, the proportion can be accurately quantified, an objective traceable basis is provided, the cost is low, the popularization rate is high, the sample treatment process is applicable, and a safe and reliable scheme is provided for high-value utilization and quality control of by-products in the citrus industry.

The utility model discloses a crop rhizome lifting structure which comprises a lifting mechanism, the lifting mechanism is arranged between a soil screening mechanism and a smashing mechanism, the lifting mechanism comprises a lifting frame and a lifting belt, the lifting frame is obliquely arranged upwards, the lifting belt is arranged in the lifting frame, the lifting belt is connected with a power system, and the power system is connected with the lifting mechanism. According to the crop rhizome lifting structure, the crop rhizomes with the large weight are smoothly lifted into a smashing mechanism, the situation that the heavy rhizomes cannot be lifted is avoided, and the working efficiency and the working quality of agricultural machinery are greatly improved.

The invention discloses an establishment method of a cherry rootstock Gisela 6 leaf in-vitro regeneration system and transgenic application thereof, and belongs to the field of plant biotechnology and genetic engineering. According to the regeneration system established by optimizing the formula of the culture medium, the regeneration frequency of the 'Gisela 6' leaf is stabilized at a relatively high level, and the quality of the regenerated material is remarkably improved. The callus generated by induction of the method provided by the invention is compact and strong in browning resistance, and the transgenic callus capable of stably expressing RUBY is obtained by a leaf disc transformation method; meanwhile, vitrification of regenerated buds is effectively inhibited, robust plants easy to transplant are obtained, and technical support is provided for precise improvement and breeding speed.

The invention discloses a mountain land wind-resistant and drought-resistant persimmon tree planting method which comprises the following steps: transplanting mountain land persimmon tree grafted seedlings in spring of the first year, planting at least one rootstock seed seedling which just germinates and does not damage radicles around the grafted seedlings while planting the persimmon tree grafted seedlings or in advance, and cultivating the rootstock seed seedling into rootstock ground seedlings after one year; in spring of the next year, the rootstock ground seedlings are inarching to nearby grafted seedling trunks; before successful inarching, the planted grafted seedlings are supported manually in an auxiliary mode to resist wind. The technical problem that persimmon trees transplanted through an existing persimmon tree grafted seedling transplanting method are poor in wind resistance and drought resistance can be solved.

The present application relates to a kind of efficient breeding methods of high resistance larch new germplasm of pine moth, including larch high resistance scion collection, rootstock selection and processing, grafting breeding and grafting management etc., in the process of high resistance scion seedling breeding, by scientific seed winter storage, sowing seedling and seedling management etc., to obtain a large number of healthy high resistance scion tender shoot provides guarantee. Scion uses the tender shoot of 10-15 days growth on seedling, and the high adaptability larch seedling of 10-15 days seedling stem straight thick, top bud full, root system developed, no disease and injury is selected as rootstock, so that grafting joint can heal quickly, shorten breeding cycle. After grafting, first grow in the environment very stable plant growth room for about 2 months, etc. Scion survival, transition period is transferred into greenhouse and grows for 1 year to reach healthy seedling, finally, further seedling raising in outdoor open-air test ground, so that seedling is fully lignified, thus a large number of high resistance pine moth larch seedling resources for artificial afforestation are obtained. The present application reduces the probability of occurrence of insect pests from the source, improves the insect resistance of larch itself, and improves the forest quality, growth rate and wood utilization efficiency.

The invention relates to the field of grafting, and discloses a peony grafting device for megaphanerophytes and a grafting technology.The peony grafting device for the megaphanerophytes comprises a mounting frame, a two-way lead screw is rotationally connected to the interior of the mounting frame, a worm gear is fixedly connected to the middle of the two-way lead screw, and the tooth end of the worm gear is connected with a gear ring in an engaged mode; the top of the gear ring is fixedly connected with an annular sliding block, the outer wall of the annular sliding block is slidably connected into the annular sliding groove, the top of the gear ring is fixedly connected with a supporting column, the bottom of the supporting column is rotatably connected with a connecting rod, the interior of the mounting frame is rotatably connected with a rotating shaft, and a grafting film coiled material is arranged on the outer wall of the rotating shaft. The free end of the grafting film coiled material is wound on the outer walls of the scion and the stock, the gear ring drives the grafting film coiled material to rotate around the outer wall of the grafting connector of the megaphanerophyte stock and the peony scion through rotation of the worm wheel, sealing operation of the connector is achieved, and therefore the grafting survival rate is increased.

The invention discloses a grafting method of a lantern tree, and relates to the technical field of plant grafting, and the main points of the technical scheme are that the grafting method comprises the following steps: S1, rootstock selection and treatment: selecting a pendant flower as a rootstock, cutting off the upper part of the rootstock, vertically and downwards cutting a notch on one side of the cross section of the rootstock, and forming a cut-grafting interface with the depth just reaching the xylem; s2, scion selection and treatment: selecting a lantern tree qualitative branch as a scion, cutting the scion into branch sections, cutting a long cut surface at the lower end of the scion by using a knife to expose a cambium, and cutting a short cut surface on the opposite side of the long cut surface to enable the lower end of the scion to be wedge-shaped; s3, grafting operation: inserting the pretreated long cut surface of the scion into a pre-cut vertical cut of the stock, so that the long cut surface of the scion is attached to a cambium on at least one side of the stock; a plastic film is wound upwards from the lower portion of the connector, and the combination portion of the stock and the scion is tightly bound and fixed and bagged. The invention fills up the blank of research in the field and is expected to cultivate a more heat-resistant lantern tree variety.

The present application relates to the technical field of micro-grafting, and provides a Xinggongguo seedling micro-grafting device and method, wherein the Xinggongguo seedling micro-grafting device comprises a fixing frame, a supporting plate, a displacement assembly, a top shell, a clamping assembly and a cutting assembly; the supporting plate is fixedly installed on the fixing frame; the displacement assembly is installed inside the fixing frame and is used for adjusting the position of the seedling to be grafted; the top shell is rotatably installed on the top of the fixing frame; the clamping assembly is installed on the supporting plate and the displacement assembly and is used for fixing the positions of the seedling to be grafted and the stock; and the cutting assembly is installed on the supporting plate and is used for cutting grooves in the clamped seedling to be grafted and the stock. Through the above technical scheme, the problem of low grafting success rate caused by the high possibility of infection of pathogenic bacteria due to the phenomenon that the grooves are not flush when cutting the small stock and scion in the prior art is solved.

The invention relates to a composition for expanding and increasing yield of rhizome crops, a soluble concentrate and application of the soluble concentrate, and belongs to the technical field of plant growth regulators. The composition consists of a prohexadione active substance and a dihydrojasmonic acid active substance, the mass ratio of the prohexadione active substance to the dihydrojasmonic acid active substance is (0.5-25): 1; the prohexadione active substance is prohexadione and / or a soluble salt thereof; the dihydrojasmonic acid active substance is dihydrojasmonic acid and / or soluble salt thereof. The composition is taken as an effective component, a growth regulator is provided, water is taken as a solvent, and auxiliary agents known by technicians in the field are supplemented, so that a soluble concentrate is prepared. The soluble concentrate has the advantages of being environmentally friendly, convenient to process, convenient to use and the like while efficiently achieving swelling and yield increasing effects of root crops, and the problems that special dosage forms need to be prepared in the using process of prohexadione calcium, so that the production cost is high, and use is inconvenient are effectively solved.

This invention discloses a small-scale fully automatic eggplant seedling grafting machine. It includes a first frame (1); the first frame (1) is equipped with a rootstock clamping and cutting mechanism (2) and a scion clamping mechanism (4); a rootstock conveying mechanism (3) is connected to one side of the rootstock clamping and cutting mechanism (2), and a grafting rootstock mechanism (7) is connected to the output end of the rootstock conveying mechanism (3); a scion transfer mechanism (5) is provided to one side of the scion clamping mechanism (4), and a grafting scion mechanism (8) is connected to the side of the scion transfer mechanism (5); a grafting mechanism (6) is connected to the same side of the grafting rootstock mechanism (7) and the grafting scion mechanism (8), and a vibrating disc (12) is provided on the side of the grafting mechanism (6), with a grafting sleeve (14) installed in the vibrating disc (12). This invention can improve grafting efficiency and has the advantages of low cost, high efficiency, and labor saving.

The application relates to the field of plant molecular breeding and low-temperature stress cultivation technology, and particularly relates to application of a CmoCK1 gene in creating a low-temperature-resistant rootstock of cucumber; through overexpression of the CmoCK1 gene and low-temperature treatment of a wild-type cucumber, the low-temperature resistance of the cucumber with overexpression of the CmoCK1 gene is significantly improved; the wild type and the overexpression plant are grafted as rootstocks to wild-type scions respectively, it is found that the cucumber with overexpression of the CmoCK1 gene as the rootstock can improve the low-temperature resistance of the scion cucumber, and the physiological indexes such as the relative conductivity and the malondialdehyde content of the above plants are determined, which shows that the new function of the CmoCK1 gene has good application potential, provides a gene resource and a new idea for reducing low-temperature stress, delaying the low-temperature response of the cucumber and further cultivating a low-temperature-resistant special rootstock of the cucumber in production, and has a wide application prospect and high use value.

The invention relates to the technical field of fruit tree cultivation, and discloses an apple deep-rooted cultivation method suitable for M9T337 rootstock, the apple deep-rooted cultivation method comprises the steps of nursery stock pretreatment, ditching and field planting, staged soil covering and chemical control management, rootstock cortex rooting is induced by combining layer-by-layer soil covering and chemical control measures, and the problems of shallow root system and poor stress resistance of the M9T337 rootstock are solved. According to the method, a complete technical chain of pretreatment, field planting, root inducing, regulation and maintenance is constructed, a continuous deep root induction environment is created through staged soil covering, and a deep root cultivation mode from passive adaptation to active induction is realized by matching with pretreatment of a composite agent to induce rooting and chemical control management to coordinate nutrient distribution. Practice shows that after M9T337 rootstock apple seedlings cultivated through the method are planted for one year, the proportion of deep root systems below 30 cm is increased to 45-50% from 15-20% of traditional cultivation, the length of main roots can reach 50-60 cm, root system distribution is more reasonable, and the deep water and fertilizer absorption capacity is remarkably improved.

The utility model discloses a plant rootstock sampling device, which comprises a structure main body (1), a lifting mechanism (2) and an electrical control system (3), the structure main body (1) comprises multiple layers of bearing parts, the lifting mechanism (2) and the electrical control system (3) are respectively arranged on the multiple layers of bearing parts of the structure main body (1), the lifting mechanism (2) is arranged at the lower end of the electrical control system (3), and the electrical control system (3) is arranged on the lifting mechanism (2). And the controller is connected with the electrical control system (3). According to the utility model, a mechanical mode is adopted to replace a manual mode, customs personnel lift the sampling device to a position above a sampling point through a crane outside the storage cabin, and the sampling device is provided with a trigger mechanism and can automatically carry out sampling operation, so that not only is the personnel operation convenient, the sampling efficiency is improved, the human resource is saved, but also the occurrence of safety accidents is avoided.

This invention relates to the field of grape cultivation technology, and particularly to a method for dual-control regulation of Crimson grape growth, comprising the following steps: Step S1: Crimson grape grafting; Step S11: Preparing the Zhichang Anti-Growth Rootstock No. 1, Victoria Interplant, and Crimson scion; Step S12: Grafting is carried out in spring at 10-20℃ using cleft grafting; Step S13: After grafting, conventional field management methods are adopted; Step S2: Crimson grape grafted seedlings are planted and shaped. This invention utilizes a rootstock-interplant-scion grafting complex combined with trellis adjustments to induce drooping of new shoots to simultaneously regulate the growth of Crimson grapes. This enhances the stress resistance of Crimson grapes, promotes nutrient production, reduces nutrient waste, improves nutrient conversion and utilization efficiency, and lowers labor costs. It can reduce workload, lower labor costs, improve nutrient utilization efficiency, and increase economic benefits.

The fruit tree grafting assistor with the automatic positioning function comprises an outer lantern ring, a position adjusting sliding groove is formed in the inner wall of the outer lantern ring, the front end of the outer lantern ring is connected with an inner lantern ring in an inserted mode through the position adjusting sliding groove, and the outer lantern ring and the inner lantern ring form a plurality of fixing sleeve pieces. The upper side of the center of the rear end of the outer lantern ring and the upper side of the center of the front end of the inner lantern ring are both connected with angle adjusting bases, angle adjusting insertion holes vertically penetrate through the interiors of the two angle adjusting bases, the lower ends of the two angle adjusting bases are both connected with angle adjusting pull belts, and the two angle adjusting pull belts are located between the two angle adjusting insertion holes. The telescopic nested lantern ring design is adopted, diameter adjustment is achieved through sliding fit of the inner lantern ring and the outer lantern ring, scions and rootstocks of different thicknesses can be tightly attached, and the adaptive capacity is improved. The telescopic nested type lantern ring has the advantages that the telescopic nested type lantern ring design is adopted, the diameter adjustment is achieved through sliding fit of the inner lantern ring and the outer lantern ring, and the telescopic nested type lantern ring can be tightly attached to the scions and the rootstocks of different thicknesses. The anti-skid latch structure on the inner wall of the lantern ring ensures firm fixation, avoids displacement, and improves the fixing stability.

This invention discloses a method for improving the asexual propagation of the new double-flowered crape myrtle variety 'Yunshang', comprising the following steps: (1) selecting a suitable grafting time; (2) selecting a suitable type of grafting rootstock; (3) selecting a suitable scion; and (4) selecting a suitable grafting method. By optimizing relevant process parameters using the method of this invention, the grafting survival rate of the new double-flowered crape myrtle variety 'Yunshang' is extremely high (96.67%), and its growth rate is also relatively fast (i.e., an annual growth of 167.68 cm). This allows for the rapid propagation of a large number of double-flowered crape myrtle 'Yunshang' seedlings, with excellent seedling quality. This method can maintain the superior traits of the new double-flowered crape myrtle variety 'Yunshang', meet the grafting requirements of the new double-flowered crape myrtle variety 'Yunshang', and quickly obtain high-quality seedlings of the new double-flowered crape myrtle variety 'Yunshang', which can be widely promoted and applied.

The present application relates to a method for improving the cold resistance of grape, which comprises any one of the following modes or a combination thereof: (a) inducing the synthesis of endogenous resveratrol in grape scion; (b) spraying exogenous resveratrol on grape seedling; (c) selecting rootstock varieties with higher resveratrol synthesis level; (d) knocking out / silencing the VvERF113 gene of the scion by gene editing technology; (e) using rootstock varieties with down-regulated VvERF113 gene expression; (f) increasing the expression of VvSTS gene of the scion or rootstock. The present application improves the cold resistance of grape seedling by regulating the content of resveratrol in grape seedling, and has the advantages of fast transformation speed, stable cold resistance, good environmental protection, etc. for non-cold-resistant seedlings.

Methods, devices, and a system for growing and germinating seeds, clones, rootstock, and mature plants use a unique yet efficient elastic force-based method in high-production hydroponics. The application of this elastic force-based holding technique results in several advantages: improved root health through the elimination of root strangulation and binding, simplified plant removal for environmental friendliness, enhanced hydroponic gardening efficiency and versatility, minimized shipping weight, compatibility with standard industry gardens, and reduction of root-bound growth and associated issues. In one example, a unique method of using a thin film to create a flexible hydroponic garden that is equipped with an optional elastic method to retain vegetative plants.

This invention provides a comprehensive fertilization method to promote the rapid growth of Podocarpus macrophyllus, including step S1: Before planting the Podocarpus macrophyllus, determine the amount of fertilizer, the depth of the planting hole, and ensure that the diameter of the planting hole is larger than the diameter of the root ball of the Podocarpus macrophyllus, based on the diameter of the pine tree's rootstock. Step S2: Mix the fertilizer combination that meets the required amount of fertilizer with the soil excavated from the planting hole to form a soil-fertilizer mixture. When planting the Podocarpus macrophyllus, use the soil-fertilizer mixture to bury the root ball of the Podocarpus macrophyllus in the planting hole. Step S3: During the growing season of the Podocarpus macrophyllus, use a combination of short-acting and long-acting fertilizers to promote its rapid growth. This invention can provide optimal fertilizer efficiency for Podocarpus macrophyllus and promote its rapid growth.

This invention belongs to the field of agricultural biotechnology, specifically relating to a strain of Pseudomonas sp. R10 and its applications. The Pseudomonas sp. R10 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC M 2024850. This strain exhibits significant control effects against clubroot disease, particularly in rapeseed. Verification through examples shows that Pseudomonas sp. R10 inhibits the germination rate of dormant spores of Pseudomonas by 35.14%. In pot experiments, it reduced the clubroot disease severity index by 53.06%. In field trials, treatment of rapeseed with R10 solution reduced the clubroot disease severity index by 39.45%, and significantly increased plant height, rootstock diameter, fresh weight, number of effective branches, and number of effective siliques in the main inflorescence compared to the control plot. R10 solution treatment also increased rapeseed yield by 59.86% and thousand-grain weight by 7.25%.

This invention provides a cold-resistance gene containing a BRZL domain isolated and cloned from pear rootstock. PbbZIP44 This study also provides insights into the application of this gene in enhancing plant cold resistance. Through the construction of... PbbZIP44 Gene overexpression and silencing vectors were developed and introduced into Arabidopsis thaliana, *Pyrus pyrifolia* callus, and *Pyrus pyrifolia* seedlings using Agrobacterium-mediated genetic transformation. Stable and transient expression were achieved, resulting in stable overexpressing plants and TRV-mediated silencing lines for low-temperature resistance functional analysis. Results showed that under low-temperature stress, the overexpressing lines exhibited stronger growth, lower malondialdehyde content and relative conductivity, as well as higher photosynthetic efficiency and antioxidant capacity compared to wild-type plants; while the silencing lines showed the opposite physiological response.

The invention discloses a tissue culture medium for rapid seedling raising of apple rootstocks and a rapid seedling raising method, and belongs to the technical field of plant tissue culture. The method specifically comprises the following steps: (1) obtaining a sterile material; (2) induction culture: inoculating the treated explants into an induction culture medium, and culturing at room temperature for 15-20 days to obtain adventitious buds; (3) multiplication culture: inoculating the adventitious buds into a multiplication culture medium, and carrying out light-dark culture at room temperature for 25-30 days to obtain cluster buds; (4) rooting culture: inoculating the cluster buds into a rooting culture medium, and carrying out light-dark culture at room temperature until the root length is 2-4cm; (5) transplanting. Through cooperation of the specific induction culture medium, the proliferation culture medium and the rooting culture medium, the growth and development rate and the survival rate of the apple rootstock can be effectively increased.

The invention belongs to the field of tomato seedling cultivation, and particularly relates to a method for accurately evaluating the level of tomato rootstock resistance to elephantopus scaber root-knot nematode. On the basis of testing indexes such as a disease index, a root knot index, an egg index, a propagation coefficient and a soil microorganism species number of tomato rootstocks, a rootstock growth index is combined, an index (Simpson index) algorithm of species diversity in a soil microorganism community is creatively utilized, evaluation parameters are optimized, and the evaluation accuracy is improved. The measured indexes are adopted to calculate membership functions and soil microbial diversity indexes to evaluate the resistance of the tomato rootstock to be detected to the root-knot nematode, so that the rootstock variety for resisting the tomato rootstock to the root-knot nematode can be better and comprehensively clustered and analyzed, and the resistance of the tomato rootstock to the tomato rootstock to the root-knot nematode can be accurately evaluated; and a novel method is provided for screening the tomato rootstock for resisting the rhus chinensis root-knot nematode.

The application relates to the technical field of plant grafting, and particularly discloses a grafting method capable of improving disease resistance of eggplants, which comprises the following steps: Solanum nigrum seedling, eggplant seedling, seedling period management and grafting; in the application, the wild Solanum nigrum is selected as the stock, has the advantages of fast growth speed and good disease resistance, and can greatly improve the disease resistance and yield of the plant as the stock; the eggplant has a high incidence rate during the growth process, especially the occurrence of the yellow wilt disease is serious, and the yield is completely lost in serious cases; the grafting can solve the fundamental problem; the Solanum nigrum seed has a large amount, is easy to breed, and has a high germination rate; the special nutrient solution is sprayed, the wound healing speed of the grafting is greatly accelerated, and the healing effect is good; the grafting method of the sleeve pipe is adopted, the grafting speed is improved, the wound is protected, and the bacteria are prevented from invading the wound.

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.