71 results about "Soil enzyme" patented technology

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention relates to the technical field of agricultural cultivation, in particular to a farmland soil ecological structure restoration and soil fertility improvement cultivation method which comprises the steps that 1, the soil state is evaluated; 2, tillage depth is determined; step 3, adding organic materials; 4, tillage operation is carried out, a tillage machine is used for carrying out soil tillage, the tillage depth is the tillage depth determined in the step 2, and the tillage operation adopts a layered tillage mode including shallow tillage and deep tillage; step 5, inoculating a microbial agent, namely inoculating the soil microbial agent based on the soil microbial diversity and the soil enzyme activity in the step 1 after the cultivation operation; 6, covering and maintaining are conducted, after cultivation and inoculation, soil covering and maintaining are conducted, and using of covering materials and precise water and fertilizer management are included. Accurate soil evaluation and personalized tillage depth determination enable soil remediation to be more targeted and effective; organic materials and microorganisms are reasonably matched and used, so that the soil fertility and the biological activity are improved.

The invention relates to the technical field of forest fertilization, in particular to a forest precise fertilization decision-making method and system based on soil nutrient profit and loss, and the method comprises the steps: obtaining basic information of a target forest land, and dividing forest growth stages based on the basic information; the method comprises the following steps: collecting an undisturbed soil sample and a mixed soil sample of a 0-40cm soil layer of a target forest land by adopting an S-shaped point distribution method, separating the mixed soil sample, and respectively measuring the soil nutrient content, the bioavailability, the soil enzyme activity and the microbial functional gene. According to the method, by measuring the dimension indexes, constructing a five-dimensional profit and loss evaluation index system for determining the reference value and dynamically adjusting the weight according to the forest land data, calculating the comprehensive profit and loss index, judging the nutrient state and generating the personalized fertilization scheme, the defects that an existing fertilization scheme is single in basis index and lacks an effective dynamic adjustment link are effectively overcome; the requirements of multi-generation continuous planting forest land are met, and the environmental risks such as fertilizer waste, soil acidification and water eutrophication are effectively reduced.

The invention discloses a micro-fluidic chip for soil enzyme activity detection and application of the micro-fluidic chip, and belongs to the technical field of soil enzyme activity detection. The whole micro-fluidic chip is circular and comprises an upper layer and a lower layer which are bonded, and a plurality of same detection units are distributed around the circle center of the micro-fluidic chip between the upper layer and the lower layer; each set of detection unit comprises a V-shaped soil cavity, a reagent cavity, a reaction cavity and a detection cavity which are sequentially arranged from the circle center to the circumference; except that the tops of two adjacent arms of the two V-shaped soil cavities are respectively provided with a liquid injection hole and an exhaust hole of a soil suspension, the top ends of two adjacent arms of the other V-shaped soil cavities are communicated, so that all the V-shaped soil cavities form a through cavity; the bottom of the V-shaped soil cavity is communicated with the reaction cavity through a micro-channel provided with a capillary valve, and the bottom of the reagent cavity is also communicated with the reaction cavity through a micro-channel provided with a capillary valve; a reagent filling hole is formed in the top of the reagent cavity; the middle lower part of the reaction cavity is communicated with the detection cavity through a siphon valve, the near-circle-center end of the detection cavity is provided with a micro-channel extending towards the circle center direction, and the tail end of the detection cavity is provided with a vent hole. The micro-fluidic chip provided by the invention is used for detecting the enzyme activity of soil and has the advantages of simplicity in operation, high accuracy, large flux and better repeatability.

The invention provides a charcoal-based microalgae fertilizer as well as a preparation method and application thereof, the method adopts a mode of co-fermentation of charcoal and microalgae to fix microalgae on charcoal, and co-fermentation is performed to prepare the charcoal-based microalgae fertilizer. The biochar-based microalgae fertilizer can significantly improve the crop yield, increase the chlorophyll content of crops, promote healthy growth of plants, optimize the pH value of soil, enhance the water and fertilizer retention capacity of soil, and significantly improve the soil nutrients and soil enzyme activity, and has significant advantages in the aspects of nutrition supply, soil health, environmental protection, crop quality improvement and the like. The biochar-based microalgae fertilizer has two forms of liquid and dry powder, can be flexibly used according to a specific agricultural production mode, has the advantages of easily available raw materials and simple preparation, is a sustainable fertilizer source, can comprehensively improve the soil fertility and the crop stress resistance, reduces the dependence on chemical fertilizers, and reduces the environmental pollution problem in agricultural production.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.

The invention provides Burkholderia cepacia and application thereof, the Burkholderia cepacia is preserved in China General Microbiological Culture Collection Center on October 8, 2021, the preservation number is CGMCC No.23540, the classification name is Burkholderia cepacia strain ZG7, the Burkholderia cepacia and medicago sativa are combined to repair the enzyme activity of heavy metal contaminated soil, and the Burkholderia cepacia strain ZG7 can be applied to soil remediation. The Burkholderia cepacia is applied to a Burkholderia cepacia planting area, soil enzyme activity is improved, a heavy metal contaminated site is repaired, the Burkholderia cepacia is applied to the Burkholderia cepacia planting area, the dry weight, chlorophyll content and antioxidant enzyme SOD and POD activity of Burkholderia cepacia plants are improved, and the toxic effect of heavy metal on the plants is reduced.

The invention discloses a nano-material-based nitrogen-phosphorus-potassium compound fertilizer as well as a preparation method and application thereof, and belongs to the field of plant nutrition and soil science research. According to the invention, the nano biochar prepared through nano grinding after pyrolysis of the rice straw is directly mixed with three chemical components, namely the urea, the ammonium dihydrogen phosphate and the potassium chloride, and the sodium bentonite, and the key points of the effects are triple effects of promoting early growth and rapid ripening, enhancing tillering and advancing heading. After the nano biochar is matched with balanced NPK nutrition (such as NPK-II), the activity level of soil key enzymes, including catalase (CAT), alkaline phosphatase (AKP), urease (UE), sucrase (SC) and the like, is remarkably improved. Besides, the composite particles are constructed through the cooperation of the nano-charcoal and the bentonite, and a low-temperature drying process is adopted, so that moisture absorption and caking can be inhibited, and the storage stability and field application performance of the fertilizer are remarkably improved.

The invention discloses an application of a biochar-loaded B. subculis microbial inoculum in prevention and treatment of celery continuous cropping obstacles and a preparation method of the biochar-loaded B. subculis microbial inoculum. Belongs to the technical field of successive cropping obstacles. The problem that continuous cropping causes reduction of crop yield and soil fertility is an urgent problem to be solved in vegetable production, the research of the invention proves that the application of the biochar-loaded B.subtilis microbial agent can improve soil fertility, effectively promote growth of celery, improve soil physicochemical properties and soil enzyme activity, and the biochar-loaded B.subtilis microbial agent is expected to become a potential soil conditioner to relieve the problem of continuous cropping of soil.

The invention relates to the technical field of soil remediation, in particular to an iron-modified cow dung biochar material and a preparation method thereof and application in remediation of cadmium-copper contaminated soil. Cow dung is used as a raw material, pretreated cow dung is obtained through natural airing, dewatering and smashing, then the pretreated cow dung and a ferrous chloride solution are mixed, stirred, filtered, dried and ground, and the iron-modified cow dung biochar material is obtained. And pyrolyzing under the protection of inert gas. The iron-modified biochar has a porous layered structure, rich oxygen-containing functional groups and iron-based active sites, has a pH value close to neutrality, and can effectively adapt to the farmland soil environment. The material can remarkably reduce the effective state content of cadmium and copper in soil, promote conversion of heavy metal to a stable residue state, reduce migration of heavy metal in pore water and improve the enzyme activity and nutrient effectiveness of the soil at the same time, the grain cadmium accumulation reduction amplitude reaches 71.65% and the copper accumulation reduction amplitude reaches 29.36% in the rice growth process, and therefore the cadmium and copper combined polluted soil is efficiently repaired, and the quality of the rice is improved. And crop safety and soil health are guaranteed.

The invention belongs to the technical field of soil conservation and tobacco cultivation, and particularly relates to an inoculant containing arbuscular mycorrhizal fungi (AMF) and application of the inoculant in relieving continuous cropping obstacles of tobacco. The arbuscular mycorrhizal fungi in the inoculant containing the arbuscular mycorrhizal fungi are inoculated to the root of tobacco, so that the agronomic traits of the tobacco can be improved, the photosynthetic capacity of the tobacco is improved, and the biomass of the tobacco is increased. Meanwhile, the agrochemical character of continuous cropping soil is improved through the arbuscular mycorrhizal fungi, the content of available phosphorus in tobacco soil is increased, and the content of organic matter and available potassium in the soil is reduced; the soil enzyme activity is remarkably improved, so that soil nutrients are regulated and controlled, absorption and utilization of nitrogen, phosphorus and potassium nutrients in the soil by tobacco are promoted, and a theoretical basis and technical support are provided for promoting tobacco growth and continuous cropping soil improvement by using a mycorrhiza technology.

The application relates to the technical field of image processing, in particular to a soil enzyme activity detection and analysis system based on intelligent sensing technology, which comprises a time determination unit, which is used for determining a detection time according to historical fluorescently labeled substrate reaction data in a soil enzyme activity historical detection data table; wherein the historical fluorescently labeled substrate reaction data comprises enzyme activity benchmark data in an initial construction period of a target detection area and fluorescently labeled substrate reaction data collected in a crop growth process of the target detection area. The initial construction period benchmark data and the crop growth process data in the historical fluorescently labeled substrate reaction data are used for dynamically determining the detection time, so that a key time node of enzyme activity change, such as a peak period after fertilization or a change period after rainfall, can be accurately captured, the missed detection or inefficient detection of the activity peak caused by fixed-time sampling is avoided, and therefore the timeliness and representativeness of soil enzyme activity detection are significantly improved.

The invention discloses a water-saving and emission-reducing grass-growing strip-shaped intercropping and interplanting method for a wheat and corn system in a groundwater limited mining area in North China Plain. The method comprises the following steps: firstly, performing strip-shaped simultaneous sowing on wheat and clover, only performing accurate water and fertilizer regulation and control on a wheat strip in a wheat growth period, and interplanting corn in the clover strip in a no-tillage manner when the wheat enters a dough stage; after the crops are harvested, the straw is smashed, covered and returned to the field, the clover naturally overwinter, and the next planting cycle is carried out; by means of the planting method, annual water consumption and carbon and nitrogen footprint can be effectively reduced, annual grain yield stability is maintained, meanwhile, soil enzyme activity and microbial environment are improved, cooperation of water saving, emission reduction, stable yield and soil improvement is achieved, and technical support is provided for agricultural sustainable development of underground water mining limit areas.

This invention discloses a method for preparing an attapulgite-based acidic soil remediation agent. The method includes: (1) mixing urea solution and biochar and reacting them hydrothermally to obtain urea-modified biochar; (2) mixing attapulgite, calcium silicate, and urea-modified biochar to obtain the attapulgite-based acidic soil remediation agent. Compared with the prior art, applying the remediation agent of this invention to acidic soil in a single application can not only improve various physicochemical indicators such as soil pH, electrical conductivity, water holding capacity, alkaline nitrogen, available phosphorus, available potassium, soil organic matter, and cation exchange capacity, but also enhance soil enzyme activity and promote crop growth and development.

The invention discloses an oxygenation drip irrigation method for saline-alkali soil crops and application of the oxygenation drip irrigation method, and belongs to the technical field of agricultural irrigation. The method comprises the steps of supplying irrigation water with the dissolved oxygen content of 10-15 mg / L to roots through a drip irrigation system in the key period of vegetative growth of crops, and carrying out oxygenation drip irrigation operation for at least two times. The invention further particularly provides a method for adjusting the enzyme activity of the soil in the root zone of the crop based on the saline-alkali soil oxygenation drip irrigation and an oxygenation drip irrigation method for improving the yield of the crops in the saline-alkali soil. The core of the method lies in that the time, frequency and oxygen concentration of oxygenation irrigation are accurately controlled, the problem of oxygen deficit of the saline-alkali soil crop root zone is effectively relieved, and therefore the soil micro-ecological environment is improved, root development and plant growth are promoted, and finally collaborative improvement of the crop yield and quality is achieved.

The application discloses a propiconazole-azoxystrobin- clothianidin seed treatment suspending agent and a preparation method and application thereof, and belongs to the technical field of pesticide preparations; the agent takes propiconazole, azoxystrobin and clothianidin as active ingredients, takes beta-nicotinamide mononucleotide (NMN) as a functional antimicrobial component, and is supplemented with styrene-maleic anhydride copolymer sodium salt, fatty alcohol polyoxyethylene ether, xanthan gum, magnesium aluminum silicate, glycerol, polydimethylsiloxane defoaming agent, sodium benzoate, polyvinyl alcohol and deionized water to prepare the agent. The application is suitable for seed coating treatment for preventing and treating rice false smut, thrips and wheat sharp eyespot and aphids; NMN promotes the accumulation of phenolic compounds in the phloem of plants, and cooperates with clothianidin on the same insecticidal target, thereby significantly improving the prevention and treatment effect on aphids and thrips; the degradation product of NMN promotes the proliferation of beneficial microorganisms in the rhizosphere, improves soil enzyme activity, and realizes soil micro-ecological restoration.

The invention relates to the technical field of microorganisms, in particular to bacillus amyloliquefaciens NECC10723 and application of the bacillus amyloliquefaciens NECC10723, the preservation number of the bacillus amyloliquefaciens NECC10723 is CGMCC No.36726, the bacillus amyloliquefaciens NECC10723 is preserved in the China General Microbiological Culture Collection Center, the preservation date is November 21, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain NECC10723 can increase the content of nutrient elements in crops and promote the growth of the crops under saline-alkali stress; osmotic regulation substances in crops are increased, and osmotic balance is maintained; accumulation of ROS and hydrogen peroxide of crops is reduced, and the content of antioxidant enzymes is increased, so that the antioxidant capacity of the crops is improved; the soil enzyme activity and the soil nutrient content are improved, and conversion and utilization of nutrient components in the soil are promoted.

The invention relates to the technical field of agricultural production, and particularly discloses a humic acid biochar composite preparation and a preparation method and application thereof, and the humic acid biochar composite preparation is composed of 120-480 kg / mu of biochar and 49-51 L / mu of 100-400 mg / L of humic acid. The humic acid biochar composite preparation provided by the invention can significantly improve the activity of millet protective enzyme, improve the physical and chemical properties of soil, enhance the enzyme activity of soil, enhance the stress resistance of millet and the fertility of millet planting soil, and promote the growth and development of millet seedlings.

The application discloses a kind of time sequence in situ zymogram soil enzyme activity quantitative imaging methods based on multi-parameter screening, it is related to soil enzyme activity detection technical field, the method collects time sequence fluorescence image of enzymatic reaction, constructs pixel-level time sequence gray matrix;Multi-stage screening is carried out to pixel, including initial screening based on gray value and standard deviation, slope and goodness of fit screening of sliding window fitting, and reaction time window final screening;Extract maximum local linear growth rate as uncorrected enzyme activity, introduce diffusion correction model correction, obtain corrected enzyme activity;Quantitative conversion and visual output are realized in combination with fluorescence standard curve.This application changes static measurement into dynamic process analysis, effectively suppresses background noise, significantly improves the sensitivity, spatial resolution and quantitative accuracy of enzyme activity detection.

The application discloses a kind of anti-saline-alkali microbial inoculants and preparation method thereof, belong to the field of microbial technology.The microbial inoculants is made of composite microbial inoculants 100-150 parts, fish bone hydrolysate 10-30 parts, nano silicon sol 1-3 parts.The composite microbial inoculants is made of Trichoderma reesei, Pseudomonas plecoglossicida, Acinetobacter baumannii.The anti-saline-alkali microbial inoculants has remarkable effect on saline-alkali soil improvement, greatly reduces soil acidity, salt content and bulk density, and the yield of saline-alkali soil wheat planting is obviously improved.The soil organic matter content and soil enzyme activity are significantly improved after use, the soil quality and rhizosphere microecological environment are improved, and the enzyme activity in wheat is improved, the stress resistance is improved, the yield reduction caused by saline-alkali stress is reduced, and the use of microbial inoculants can also reduce the amount of chemical fertilizer and pesticide, reduce production cost, and further improve the economic benefit of wheat planting.

This invention provides an acid- and alkali-producing bacterial combination, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a bacterial combination including Bacillus subtilis with accession number CGMCC No. 29201. Bacillus sp S1 and Arthrobacterium with accession number CGMCC No. 29202 ( Arthrobacter sp S2. In this invention, both Bacillus S1 and Arthrobacter S2 are acid- and alkali-producing strains with strong environmental adaptability. Through synergistic effects via multiple pathways, including ammonia production, bicarbonate secretion, and activation of soil enzymes, the bacterial combination can significantly increase soil pH, reduce exchangeable aluminum and hydrolyzable acids, and simultaneously increase soil bicarbonate content, thus rapidly and comprehensively improving soil acidity from its root cause. This bacterial combination is of great significance for achieving green, efficient, and sustainable remediation of acidified soils.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

The invention belongs to the technical field of agricultural cultivation, and provides a cowpea hydrogen-rich water green cultivation method which comprises the following steps: S1, providing a cowpea planting field, and carrying out soil preparation treatment before planting; s2, applying hydrogen-rich water into the soil in the seedling stage, the early flowering stage and the harvesting stage after the cowpeas are sown; s3, in the growth period of the cowpeas, water and fertilizer integrated management is conducted according to the growth stage, and fertilizer and hydrogen-rich water are mixed for application; according to the method, the oriental long dragon cowpeas are planted in a suitable land parcel of a subtropical monsoon humid climate region, hydrogen-rich water which is diluted and used for adjusting the pH value is applied to soil after sowing, in the seedling stage, in the early flowering stage and in the harvesting stage, and root irrigation treatment is conducted in combination with water and fertilizer integrated management and pesticide; the soil enzyme activity, the growth vigor, the yield and the quality of the cowpeas are remarkably improved, meanwhile, the pest and disease damage rate and pesticide residues are reduced, and the safety and the quality of cowpea products are guaranteed.

The invention discloses a complex microbial inoculant, a biological stimulant, a preparation method and application. The composite bacterial agent disclosed by the invention comprises Pristia aryabhattai P28 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) 1.65035), bacillus subtilis S1 (the preservation number is CGMCC 1.65034) and bacillus velezensis C1 (the preservation number is CGMCC 1.65033), and the volume ratio of the P28 to the S1 to the C1 is 3 to 1 to 2. The biological stimulant is prepared from kitchen waste aerobic hydrolysate which is obtained by fermenting organic waste kitchen waste serving as a raw material with a complex microbial inoculant with the addition amount of 5% by volume. After the biological stimulant is applied to soil, the total amount of soil organisms (bacteria, fungi and protozoa) can be effectively increased, the soil aggregate stability is improved, and the soil structure is rapidly improved; the content of total organic carbon, granular organic carbon and soluble organic carbon in soil and the activity of soil enzymes (catalase, sucrase, urease and acid phosphatase) are obviously improved in a short time. The method disclosed by the invention has important significance on resource utilization of organic wastes and agricultural sustainable development.

This invention discloses a highly efficient strain of *Bacillus tekirii* BB that degrades PLA microplastics and its applications. This strain was deposited on February 2, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing, with accession number CGMCC NO. 37630. This strain can secrete multiple enzyme systems, including lipase, amylase, laccase, and catalase. In soil systems, this strain can effectively degrade PLA, increase soil lactic acid content and the activity of various soil enzymes, and improve soil organic matter, nitrogen, phosphorus, and potassium nutrient status. This strain can alleviate the growth stress of PLA pollution on plants, increasing the plant height, biomass, and antioxidant enzyme activity of crops such as buckwheat. This strain combines PLA degradation, soil remediation, and plant growth promotion functions, making it suitable for in-situ bioremediation of PLA microplastic pollution in farmland and possessing significant environmental and agricultural application value.

The invention discloses a young tea tree-soybean-corn intercropping method and application thereof in improving tea garden soil and microclimate and increasing the survival rate of tea seedlings, and belongs to the technical field of agricultural planting. According to the method, clonal national improved variety Grain Rain Spring young tea trees are used as main crops and matched with Zhenong No.6 soybeans and Liuyu No.3 corns for intercropping, and the comprehensive effects of tea garden microclimate regulation, soil nutrient improvement, soil enzyme activity enhancement and soil microbial community optimization are achieved by optimizing crop planting parameters and field management measures. According to the technology, the survival rate of the tea seedlings can be remarkably increased, and the survival rate of the tea seedlings of the intercropping group is 1.22 times that of the tea seedlings of the non-intercropping control group. An efficient and feasible planting mode is provided for ecological tea garden construction and rapid young tea garden formation, and the sustainable development capacity and economic benefits of the tea industry can be improved.