91 results about "Soil enzyme" patented technology

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention relates to the technical field of agricultural microorganisms, in particular to a microbial agent containing paenibacillus love KF-B6, a compound microbial fertilizer and application of the microbial agent and the compound microbial fertilizer. The KF-B6 Paenibacillus lovuenyuan as well as the microbial agent and the compound microbial fertilizer thereof provided by the invention can promote the formation of soil aggregates, improve soil organic matters, improve soil enzyme activity, effectively improve soil and increase the proportion of soil bacteria and fungi. Therefore, the method can be applied to prevention and treatment of apple continuous cropping obstacles, and the survival rate of field planting of apple seedling stocks and field planting of young trees in a rebuilt orchard is guaranteed. By applying the KF-B6 compound microbial fertilizer and the microbial agent, the survival rate of the M9T337 rootstock seedlings planted in the continuous cropping soil can be increased, the highest survival rate reaches 96.67%, and the seedling roots are high in activity and good in growth vigor. The survival rate of the Daphne red apple seedlings planted in the rebuilding of the continuous cropping obstacle old orchard is as high as 94.00%, and the effect of effectively preventing and controlling the apple continuous cropping obstacle is achieved.

The invention discloses a straw returning index design method, and belongs to the field of straw returning mode optimization. The problem that an existing method is poor in straw returning index optimization effect is solved. Through drift diameter analysis, the response of a soil ecosystem in the field returning decomposition process can be analyzed from four aspects of soil basic nutrients, a soil organic carbon library, a soil enzyme activity system and a soil microbial community; and by means of factor analysis modeling thinking, a soil ecology comprehensive response index is constructed, so that the influence of the straw length, the straw returning amount and the straw burial depth on a soil ecosystem is explored, the optimal returning index for promoting straw decomposition and improving the soil ecosystem is found, and the effect of optimizing the straw returning index is improved. According to the method, the straw length, the straw returning amount and the straw burial depth are optimized, and important support is provided for agricultural sustainable development. The method can be applied to straw returning optimization.

The invention relates to the technical field of microbial fertilizers, in particular to a microbial fertilizer for improving a soil structure and a preparation method thereof, and the microbial fertilizer comprises the following components: microbial flora, a charcoal-based composite material, magnetic nanoparticles, a polymer water retention agent, a soil enzyme activator, peanut bran, molasses and a trace element supplement. By selecting and proportioning functional components such as various microbial florae, biochar-based composite materials, magnetic nanoparticles and the like, the invention aims to construct the microbial fertilizer with efficient nutrient utilization, excellent soil structure improvement capability and environmental adaptability, not only the instant effect of the fertilizer is concerned, but also the long-term benefit and ecological sustainability of the fertilizer are concerned, and the application prospect is wide. Powerful support is provided for green development of modern agriculture.

The invention discloses a bacillus megaterium Q7 for promoting the growth of leucaena leucocephala and improving the abundance of a probiotic microbial population. The bacillus megaterium Q7 is a bacillus megaterium for promoting the growth of leucaena leucocephala. Belongs to the technical field of microorganisms. The bacillus megatherium Q7 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of the leucaena leucocephala on soil nutrients, thereby achieving the effect of promoting the growth of the leucaena leucocephala. After the bacillus megatherium Q7 provided by the invention is applied, the enzyme activity of soil is remarkably improved, the conversion of available nutrients in the soil is remarkably enhanced, and the nutrient consumption capacity of leucaena leucocephala is enhanced. The bacillus megaterium Q7 is applied to promote the growth and development of the leucaena leucocephala, so that the contents of soil hydrolytic nitrogen and available phosphorus are remarkably increased, and the conversion to soil nutrients is enhanced. In the genus level, the average relative abundance of bacillus treated by the bacillus atrophaeus Q7 is increased to 9.97% from 1.33%, the bacillus pseudobecomes the leucaena leucocephala soil community dominant bacteria genus, and the relative abundance of Fictibacillus is increased by about 6 times, which indicates that the inoculation of the strain Q7 enables the leucaena leucocephala soil community structure to be changed, and the leucaena leucocephala soil community dominant bacteria genus is changed. And the increase of the relative abundance of dominant bacteria is promoted. Wherein the bacillus and the Fictibacillus belong to obvious increase of relative abundance and are possibly an important factor for promoting plant growth.

The invention relates to the technical field of agricultural cultivation, in particular to a farmland soil ecological structure restoration and soil fertility improvement cultivation method which comprises the steps that 1, the soil state is evaluated; 2, tillage depth is determined; step 3, adding organic materials; 4, tillage operation is carried out, a tillage machine is used for carrying out soil tillage, the tillage depth is the tillage depth determined in the step 2, and the tillage operation adopts a layered tillage mode including shallow tillage and deep tillage; step 5, inoculating a microbial agent, namely inoculating the soil microbial agent based on the soil microbial diversity and the soil enzyme activity in the step 1 after the cultivation operation; 6, covering and maintaining are conducted, after cultivation and inoculation, soil covering and maintaining are conducted, and using of covering materials and precise water and fertilizer management are included. Accurate soil evaluation and personalized tillage depth determination enable soil remediation to be more targeted and effective; organic materials and microorganisms are reasonably matched and used, so that the soil fertility and the biological activity are improved.

The invention discloses bacillus toonensis YG1 with a growth promoting effect on leucaena leucocephala and lespedeza bicolor, and belongs to the technical field of microorganisms. The bacillus toonensis YG1 has the growth promoting effect on leucaena leucocephala and lespedeza bicolor. The bacillus orientalis YG1 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of leucaena leucocephala and lespedeza bicolor on soil nutrients, thereby achieving the effect of promoting the growth of leucaena leucocephala and lespedeza bicolor. The bacillus orientalis YG1 is applied, the biomass and available phosphorus of lespedeza leucocephala are increased by 181.73% and 50.32% respectively most obviously, and the content of hydrolytic nitrogen and the content of organic matter of lespedeza are increased by 45.18% and 48.90% respectively most obviously. Other physicochemical indexes in leucaena leucocephala soil and lespedeza soil are obviously increased, and meanwhile, the soil enzyme activities of the leucaena leucocephala soil and the lespedeza soil are In general, the improvement of soil physicochemical properties and the significant increase of soil enzyme activity by inoculating the YG1 microbial inoculum may be an important factor for promoting plant growth.

The invention provides a multi-element real-time monitoring method and system for regional environment characteristics. The method comprises the steps that sandy soil weight information, wind speed information, soil samples and vegetation information of a current region within a first preset duration are collected; based on the sandy soil weight information and the wind speed information, determining wind sand characteristics of the current area; determining a soil microbial community index and soil enzyme activity of the current area according to the soil sample; determining vegetation community characteristics of the current area according to the vegetation information; wherein the wind and sand characteristics comprise the sediment transport amount and the sediment transport potential, and the vegetation community characteristics comprise the plant species density, the total community coverage and the species fraction coverage. According to the technical scheme provided by the invention, the monitoring precision of the regional environment characteristics is improved.

The invention relates to the technical field of forest fertilization, in particular to a forest precise fertilization decision-making method and system based on soil nutrient profit and loss, and the method comprises the steps: obtaining basic information of a target forest land, and dividing forest growth stages based on the basic information; the method comprises the following steps: collecting an undisturbed soil sample and a mixed soil sample of a 0-40cm soil layer of a target forest land by adopting an S-shaped point distribution method, separating the mixed soil sample, and respectively measuring the soil nutrient content, the bioavailability, the soil enzyme activity and the microbial functional gene. According to the method, by measuring the dimension indexes, constructing a five-dimensional profit and loss evaluation index system for determining the reference value and dynamically adjusting the weight according to the forest land data, calculating the comprehensive profit and loss index, judging the nutrient state and generating the personalized fertilization scheme, the defects that an existing fertilization scheme is single in basis index and lacks an effective dynamic adjustment link are effectively overcome; the requirements of multi-generation continuous planting forest land are met, and the environmental risks such as fertilizer waste, soil acidification and water eutrophication are effectively reduced.

The invention discloses a micro-fluidic chip for soil enzyme activity detection and application of the micro-fluidic chip, and belongs to the technical field of soil enzyme activity detection. The whole micro-fluidic chip is circular and comprises an upper layer and a lower layer which are bonded, and a plurality of same detection units are distributed around the circle center of the micro-fluidic chip between the upper layer and the lower layer; each set of detection unit comprises a V-shaped soil cavity, a reagent cavity, a reaction cavity and a detection cavity which are sequentially arranged from the circle center to the circumference; except that the tops of two adjacent arms of the two V-shaped soil cavities are respectively provided with a liquid injection hole and an exhaust hole of a soil suspension, the top ends of two adjacent arms of the other V-shaped soil cavities are communicated, so that all the V-shaped soil cavities form a through cavity; the bottom of the V-shaped soil cavity is communicated with the reaction cavity through a micro-channel provided with a capillary valve, and the bottom of the reagent cavity is also communicated with the reaction cavity through a micro-channel provided with a capillary valve; a reagent filling hole is formed in the top of the reagent cavity; the middle lower part of the reaction cavity is communicated with the detection cavity through a siphon valve, the near-circle-center end of the detection cavity is provided with a micro-channel extending towards the circle center direction, and the tail end of the detection cavity is provided with a vent hole. The micro-fluidic chip provided by the invention is used for detecting the enzyme activity of soil and has the advantages of simplicity in operation, high accuracy, large flux and better repeatability.

The invention provides a charcoal-based microalgae fertilizer as well as a preparation method and application thereof, the method adopts a mode of co-fermentation of charcoal and microalgae to fix microalgae on charcoal, and co-fermentation is performed to prepare the charcoal-based microalgae fertilizer. The biochar-based microalgae fertilizer can significantly improve the crop yield, increase the chlorophyll content of crops, promote healthy growth of plants, optimize the pH value of soil, enhance the water and fertilizer retention capacity of soil, and significantly improve the soil nutrients and soil enzyme activity, and has significant advantages in the aspects of nutrition supply, soil health, environmental protection, crop quality improvement and the like. The biochar-based microalgae fertilizer has two forms of liquid and dry powder, can be flexibly used according to a specific agricultural production mode, has the advantages of easily available raw materials and simple preparation, is a sustainable fertilizer source, can comprehensively improve the soil fertility and the crop stress resistance, reduces the dependence on chemical fertilizers, and reduces the environmental pollution problem in agricultural production.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.

The invention provides Burkholderia cepacia and application thereof, the Burkholderia cepacia is preserved in China General Microbiological Culture Collection Center on October 8, 2021, the preservation number is CGMCC No.23540, the classification name is Burkholderia cepacia strain ZG7, the Burkholderia cepacia and medicago sativa are combined to repair the enzyme activity of heavy metal contaminated soil, and the Burkholderia cepacia strain ZG7 can be applied to soil remediation. The Burkholderia cepacia is applied to a Burkholderia cepacia planting area, soil enzyme activity is improved, a heavy metal contaminated site is repaired, the Burkholderia cepacia is applied to the Burkholderia cepacia planting area, the dry weight, chlorophyll content and antioxidant enzyme SOD and POD activity of Burkholderia cepacia plants are improved, and the toxic effect of heavy metal on the plants is reduced.

The invention discloses a nano-material-based nitrogen-phosphorus-potassium compound fertilizer as well as a preparation method and application thereof, and belongs to the field of plant nutrition and soil science research. According to the invention, the nano biochar prepared through nano grinding after pyrolysis of the rice straw is directly mixed with three chemical components, namely the urea, the ammonium dihydrogen phosphate and the potassium chloride, and the sodium bentonite, and the key points of the effects are triple effects of promoting early growth and rapid ripening, enhancing tillering and advancing heading. After the nano biochar is matched with balanced NPK nutrition (such as NPK-II), the activity level of soil key enzymes, including catalase (CAT), alkaline phosphatase (AKP), urease (UE), sucrase (SC) and the like, is remarkably improved. Besides, the composite particles are constructed through the cooperation of the nano-charcoal and the bentonite, and a low-temperature drying process is adopted, so that moisture absorption and caking can be inhibited, and the storage stability and field application performance of the fertilizer are remarkably improved.

The invention relates to a microbial composition for preventing and treating eggplant rhizome diseases and application thereof, and the composition comprises a) Bacillus velezensis and Aspergillus oryzae, and b) fulvic acid, alginic acid, amino acid, rape pollen polysaccharide, oyster peptide, berberine and potassium phosphite. Wherein the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M2022923, and the preservation number of the aspergillus oryzae is CCTCC NO: M2021249. The composition provided by the invention has a relatively good prevention and control effect on root and stem diseases caused by infection of Ralstonia solanacearum, Rhizoctonia solani, Verticillium dahlii, Fusarium solani, Choanephra cucurbitarum and Basidiomycetes, and when the composition is applied to soil, the enzyme activity of the soil and the enzyme activity of the root system of the eggplant can be effectively improved. The composition provided by the invention has a relatively good prevention and control effect on the root and stem diseases caused by infection of the Ralstonia solanacearum, the Rhizoctonia solani, the Verticillium dahlii, the Fusarium solani, the Choanephra cucurbitarum and the Basidiomycetes.

The invention relates to the technical field of agricultural microorganisms, in particular to paenibacillus brevis KF-B6 and application thereof, in particular to application in prevention and treatment of continuous cropping obstacles of apples. The preservation number of the paenibacillus brevis KF-B6 is CGMCC (China General Microbiological Culture Collection Center) No.32131. The paenibacillus brevis KF-B6 has the compound functions of quickly producing EPS (Expandable Polystyrene) at high yield, producing chitinase at high yield, producing water-soluble and volatile antibacterial substances, decomposing macromolecular organic matters and the like. The application of the bacterium, the microbial agent and the compound microbial fertilizer thereof can effectively improve the number of bacteria in rhizosphere soil, reduce the number of pathogenic fungi, increase the proportion of the bacteria to the fungi and promote the restoration of a soil microbial ecosystem of a continuous cropping obstacle apple orchard; the proportion of water-stable aggregates is increased, the soil organic matter content is increased, the soil enzyme activity is improved, and the soil is improved; the survival rate of planted saplings in the new variety updating process of the continuous cropping obstacle old orchard is increased, the plant root activity and plant growth are promoted, and the apple continuous cropping obstacle is comprehensively relieved and prevented.

The invention discloses an application of a biochar-loaded B. subculis microbial inoculum in prevention and treatment of celery continuous cropping obstacles and a preparation method of the biochar-loaded B. subculis microbial inoculum. Belongs to the technical field of successive cropping obstacles. The problem that continuous cropping causes reduction of crop yield and soil fertility is an urgent problem to be solved in vegetable production, the research of the invention proves that the application of the biochar-loaded B.subtilis microbial agent can improve soil fertility, effectively promote growth of celery, improve soil physicochemical properties and soil enzyme activity, and the biochar-loaded B.subtilis microbial agent is expected to become a potential soil conditioner to relieve the problem of continuous cropping of soil.

The invention discloses an irrigation method for improving the water and fertilizer utilization rate of greenhouse tomatoes, and the method adopts a method of combining a circulating aeration device with drip irrigation to control the fertilization amount, the soil water content and the irrigation amount in each period of tomato growth so as to monitor the change response of the soil environment and the migration, transformation and regulation of nutrients in a tomato root zone. By adopting an irrigation fertilization mode with a high aeration irrigation medium fertilizer level, growth of tomato plants and dry matter accumulation are promoted, root morphology distribution is improved, root activity is improved, photosynthetic indexes and chlorophyll content of the tomato plants are remarkably improved, photosynthesis is enhanced, and fruit yield, transverse diameter, longitudinal diameter and fruit quality are remarkably improved; the soil enzyme activity and the microorganism quantity of the tomato root zone are obviously improved, and the nutrient absorption accumulation amount and the nutrient absorption and utilization efficiency of tomato plants are improved.

The invention relates to the technical field of soil remediation, in particular to an iron-modified cow dung biochar material and a preparation method thereof and application in remediation of cadmium-copper contaminated soil. Cow dung is used as a raw material, pretreated cow dung is obtained through natural airing, dewatering and smashing, then the pretreated cow dung and a ferrous chloride solution are mixed, stirred, filtered, dried and ground, and the iron-modified cow dung biochar material is obtained. And pyrolyzing under the protection of inert gas. The iron-modified biochar has a porous layered structure, rich oxygen-containing functional groups and iron-based active sites, has a pH value close to neutrality, and can effectively adapt to the farmland soil environment. The material can remarkably reduce the effective state content of cadmium and copper in soil, promote conversion of heavy metal to a stable residue state, reduce migration of heavy metal in pore water and improve the enzyme activity and nutrient effectiveness of the soil at the same time, the grain cadmium accumulation reduction amplitude reaches 71.65% and the copper accumulation reduction amplitude reaches 29.36% in the rice growth process, and therefore the cadmium and copper combined polluted soil is efficiently repaired, and the quality of the rice is improved. And crop safety and soil health are guaranteed.

The invention belongs to the technical field of soil conservation and tobacco cultivation, and particularly relates to an inoculant containing arbuscular mycorrhizal fungi (AMF) and application of the inoculant in relieving continuous cropping obstacles of tobacco. The arbuscular mycorrhizal fungi in the inoculant containing the arbuscular mycorrhizal fungi are inoculated to the root of tobacco, so that the agronomic traits of the tobacco can be improved, the photosynthetic capacity of the tobacco is improved, and the biomass of the tobacco is increased. Meanwhile, the agrochemical character of continuous cropping soil is improved through the arbuscular mycorrhizal fungi, the content of available phosphorus in tobacco soil is increased, and the content of organic matter and available potassium in the soil is reduced; the soil enzyme activity is remarkably improved, so that soil nutrients are regulated and controlled, absorption and utilization of nitrogen, phosphorus and potassium nutrients in the soil by tobacco are promoted, and a theoretical basis and technical support are provided for promoting tobacco growth and continuous cropping soil improvement by using a mycorrhiza technology.

The application relates to the technical field of image processing, in particular to a soil enzyme activity detection and analysis system based on intelligent sensing technology, which comprises a time determination unit, which is used for determining a detection time according to historical fluorescently labeled substrate reaction data in a soil enzyme activity historical detection data table; wherein the historical fluorescently labeled substrate reaction data comprises enzyme activity benchmark data in an initial construction period of a target detection area and fluorescently labeled substrate reaction data collected in a crop growth process of the target detection area. The initial construction period benchmark data and the crop growth process data in the historical fluorescently labeled substrate reaction data are used for dynamically determining the detection time, so that a key time node of enzyme activity change, such as a peak period after fertilization or a change period after rainfall, can be accurately captured, the missed detection or inefficient detection of the activity peak caused by fixed-time sampling is avoided, and therefore the timeliness and representativeness of soil enzyme activity detection are significantly improved.

The invention discloses a water-saving and emission-reducing grass-growing strip-shaped intercropping and interplanting method for a wheat and corn system in a groundwater limited mining area in North China Plain. The method comprises the following steps: firstly, performing strip-shaped simultaneous sowing on wheat and clover, only performing accurate water and fertilizer regulation and control on a wheat strip in a wheat growth period, and interplanting corn in the clover strip in a no-tillage manner when the wheat enters a dough stage; after the crops are harvested, the straw is smashed, covered and returned to the field, the clover naturally overwinter, and the next planting cycle is carried out; by means of the planting method, annual water consumption and carbon and nitrogen footprint can be effectively reduced, annual grain yield stability is maintained, meanwhile, soil enzyme activity and microbial environment are improved, cooperation of water saving, emission reduction, stable yield and soil improvement is achieved, and technical support is provided for agricultural sustainable development of underground water mining limit areas.

The invention discloses an acid soil conditioner, an acid farmland soil-crop system improvement method and application thereof, and belongs to the technical field of acid soil improvement. The invention provides an acidic soil conditioner. The acidic soil conditioner is prepared from carbon adsorption polyglutamic acid and biochar. The acid soil conditioner can significantly improve the soil quality, can significantly reduce the use amount of biochar and carbon adsorption polyglutamic acid when being used for acid farmland soil improvement, and can synergistically enhance the soil enzyme activity, increase the absorption of nitrogen, phosphorus, calcium and magnesium by root systems, and improve the soil quality. The ear number per mu, the grain number per ear, the thousand seed weight, the grain protein content and the gluten content are improved, and the effect of simultaneously improving the yield and the quality of the wheat is achieved. According to the method, an efficient acid farmland crop production improvement technology is established, and technical support and theoretical reference are provided for acid farmland improvement and guarantee of grain safety production.

This invention discloses a method for preparing an attapulgite-based acidic soil remediation agent. The method includes: (1) mixing urea solution and biochar and reacting them hydrothermally to obtain urea-modified biochar; (2) mixing attapulgite, calcium silicate, and urea-modified biochar to obtain the attapulgite-based acidic soil remediation agent. Compared with the prior art, applying the remediation agent of this invention to acidic soil in a single application can not only improve various physicochemical indicators such as soil pH, electrical conductivity, water holding capacity, alkaline nitrogen, available phosphorus, available potassium, soil organic matter, and cation exchange capacity, but also enhance soil enzyme activity and promote crop growth and development.

The invention discloses an oxygenation drip irrigation method for saline-alkali soil crops and application of the oxygenation drip irrigation method, and belongs to the technical field of agricultural irrigation. The method comprises the steps of supplying irrigation water with the dissolved oxygen content of 10-15 mg / L to roots through a drip irrigation system in the key period of vegetative growth of crops, and carrying out oxygenation drip irrigation operation for at least two times. The invention further particularly provides a method for adjusting the enzyme activity of the soil in the root zone of the crop based on the saline-alkali soil oxygenation drip irrigation and an oxygenation drip irrigation method for improving the yield of the crops in the saline-alkali soil. The core of the method lies in that the time, frequency and oxygen concentration of oxygenation irrigation are accurately controlled, the problem of oxygen deficit of the saline-alkali soil crop root zone is effectively relieved, and therefore the soil micro-ecological environment is improved, root development and plant growth are promoted, and finally collaborative improvement of the crop yield and quality is achieved.

The application discloses a propiconazole-azoxystrobin- clothianidin seed treatment suspending agent and a preparation method and application thereof, and belongs to the technical field of pesticide preparations; the agent takes propiconazole, azoxystrobin and clothianidin as active ingredients, takes beta-nicotinamide mononucleotide (NMN) as a functional antimicrobial component, and is supplemented with styrene-maleic anhydride copolymer sodium salt, fatty alcohol polyoxyethylene ether, xanthan gum, magnesium aluminum silicate, glycerol, polydimethylsiloxane defoaming agent, sodium benzoate, polyvinyl alcohol and deionized water to prepare the agent. The application is suitable for seed coating treatment for preventing and treating rice false smut, thrips and wheat sharp eyespot and aphids; NMN promotes the accumulation of phenolic compounds in the phloem of plants, and cooperates with clothianidin on the same insecticidal target, thereby significantly improving the prevention and treatment effect on aphids and thrips; the degradation product of NMN promotes the proliferation of beneficial microorganisms in the rhizosphere, improves soil enzyme activity, and realizes soil micro-ecological restoration.